Youssef Chafai, Anas Raffak, Mohamed El-aalaoui, M. Sbaghi, Abdellatif Djerrari, M. Zahar
The prickly pear fruit's peel (PPP) and seed press-cake (SPC) are discarded during the pulp and the seed-oil processing. This study incorporated these two by-products in the preparation of sourdoughs and evaluated their bread-making capacities. The seed press-cake was recovered during the mechanical extraction of the seed oil, ground and used to produce S1 sourdough. The peels were milled and their puree was utilized to produce firm and liquid sourdoughs S2 and S3, respectively. The prepared sourdoughs had an average pH of 3.24 and a total titratable acidity (TTA) between 13.4 and 27.4 mL NaOH 0.1M/10g. The doughs fermented with these sourdoughs had different rising capacities (167 to 259%) and specific volumes (1.53 to 2.26 cm3/g). The sourdough-fermented doughs produced equivalent amounts of CO2. The doughs fermented with the peels sourdoughs produced more ethanol than that with press-cake sourdough. Fermented doughs had a final TTA between 7.8 and 11.5 mL NaOH 0.1M/10g and a final mean pH of 3.47. The control dough fermented with baker's yeast alone had a low final TTA of 4.5 mL NaOH 0.1M/10g and a comparable pH to that of the sourdough-fermented doughs. Our results suggest the incorporation of prickly pear by-products in sourdoughs formulation and use to inoculate bread dough to decrease food waste and improve the quality of baked products.
在果肉和籽油加工过程中，会丢弃刺梨果皮(PPP)和籽压榨饼(SPC)。本研究将这两种副产品加入到酵母的制备中，并评估了它们的面包制作能力。在机械提取种子油的过程中回收种子压榨饼，研磨后用于生产S1酵母。将果皮磨碎，用果浆分别制成硬曲S2和液体曲S3。制备的酵母平均pH为3.24，总可滴定酸度(TTA)在13.4 ~ 27.4 mL NaOH 0.1M/10g之间。发酵后的面团膨升率(167 ~ 259%)和比容(1.53 ~ 2.26 cm3/g)不同。发酵的面团产生了等量的二氧化碳。用果皮酵母发酵的面团比用压饼酵母发酵的面团产生更多的乙醇。发酵面团的最终TTA在7.8 ~ 11.5 mL NaOH 0.1M/10g之间，最终平均pH为3.47。单独使用面包酵母发酵的对照面团的最终TTA较低，为4.5 mL NaOH 0.1M/10g, pH值与酵母发酵面团相当。研究结果表明，在酵母配方中加入刺梨副产物并用于接种面包面团，可以减少食物浪费，提高烘焙产品的质量。
{"title":"VALORIZATION OF PRICKLY PEAR PEELS & SEED PRESS-CAKE IN TRADITIONAL SOURDOUGHS AND EVALUATION OF THEIR BREAD-MAKING CAPACITIES","authors":"Youssef Chafai, Anas Raffak, Mohamed El-aalaoui, M. Sbaghi, Abdellatif Djerrari, M. Zahar","doi":"10.55251/jmbfs.9744","DOIUrl":"https://doi.org/10.55251/jmbfs.9744","url":null,"abstract":"The prickly pear fruit's peel (PPP) and seed press-cake (SPC) are discarded during the pulp and the seed-oil processing. This study incorporated these two by-products in the preparation of sourdoughs and evaluated their bread-making capacities. The seed press-cake was recovered during the mechanical extraction of the seed oil, ground and used to produce S1 sourdough. The peels were milled and their puree was utilized to produce firm and liquid sourdoughs S2 and S3, respectively. The prepared sourdoughs had an average pH of 3.24 and a total titratable acidity (TTA) between 13.4 and 27.4 mL NaOH 0.1M/10g. The doughs fermented with these sourdoughs had different rising capacities (167 to 259%) and specific volumes (1.53 to 2.26 cm3/g). The sourdough-fermented doughs produced equivalent amounts of CO2. The doughs fermented with the peels sourdoughs produced more ethanol than that with press-cake sourdough. Fermented doughs had a final TTA between 7.8 and 11.5 mL NaOH 0.1M/10g and a final mean pH of 3.47. The control dough fermented with baker's yeast alone had a low final TTA of 4.5 mL NaOH 0.1M/10g and a comparable pH to that of the sourdough-fermented doughs. Our results suggest the incorporation of prickly pear by-products in sourdoughs formulation and use to inoculate bread dough to decrease food waste and improve the quality of baked products.","PeriodicalId":16348,"journal":{"name":"Journal of microbiology, biotechnology and food sciences","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43996612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lenka Hutárová, Miroslava Hlebová, D. Vešelényiová, Ivona Krajčoviechová, O. Strunecký
Antibiotics in the environment represent a significant pollutant with a great impact on the biota. With the increasing use of these substances, the resistance against them notably grows. The phototrophs, as the key part of microbial communities, often have different responses to antibiotics. Some species could be inhibited; on the other hand, some species show the ability to use antibiotics as a source of necessary nutrients. In our study, we investigated the impact of streptomycin, gentamicin, and sulfacetamide on six strains of cyanobacteria commonly present in water sources. The growth inhibitory effect of the studied antibiotics was measured in sterile 96-well microtiter plates, which contained different concentrations of antibiotics (1024 – 0.5 µg/mL) during 7 days at 23 °C and with 24h illumination (light intensity 48,6 µmol. foton m-2 s-1). Then the results were statistically evaluated, and growth curves were designed for each studied strain. The minimum inhibitory concentration (MIC) of the investigated antibiotics was evaluated using probit analysis. The potential effect of antibiotics on tested strains morphology was also studied. The results show that the antibiotics have an inhibitory effect at higher concentrations (891.76 μg/mL to 1495.10 μg/mL). The highest values of MIC were estimated for Synechocystis sp. (PCC 6803) and Cephalotrix sp. (KL 18). The stimulation of growth was observed in the strains Synechocystis (PCC 6803) and Cephalothrix (KL 18). The most sensitive strains to selected antibiotics were Chlorogleopsis fritschii (CCALA 1005), Lyngbya martensiana (CCALA 930), and Geitlerinema acuminatum (CCALA 141). Strain Cyanobium sp. (LH14) was sensitive to streptomycin sulphate and gentamicin sulphate, but sulfacetamide stimulated its growth. The visible morphological changes were caused by streptomycin in Chlorogloeopsis fritschii (CCALA 1005). After five days of cultivation the bleached cells were present in the cultures of this strain.
{"title":"EFFECT OF SELECTED ANTIBIOTICS ON THE GROWTH AND MORPHOLOGY OF CYANOBACTERIA","authors":"Lenka Hutárová, Miroslava Hlebová, D. Vešelényiová, Ivona Krajčoviechová, O. Strunecký","doi":"10.55251/jmbfs.10221","DOIUrl":"https://doi.org/10.55251/jmbfs.10221","url":null,"abstract":"Antibiotics in the environment represent a significant pollutant with a great impact on the biota. With the increasing use of these substances, the resistance against them notably grows. The phototrophs, as the key part of microbial communities, often have different responses to antibiotics. Some species could be inhibited; on the other hand, some species show the ability to use antibiotics as a source of necessary nutrients. In our study, we investigated the impact of streptomycin, gentamicin, and sulfacetamide on six strains of cyanobacteria commonly present in water sources. The growth inhibitory effect of the studied antibiotics was measured in sterile 96-well microtiter plates, which contained different concentrations of antibiotics (1024 – 0.5 µg/mL) during 7 days at 23 °C and with 24h illumination (light intensity 48,6 µmol. foton m-2 s-1). Then the results were statistically evaluated, and growth curves were designed for each studied strain. The minimum inhibitory concentration (MIC) of the investigated antibiotics was evaluated using probit analysis. The potential effect of antibiotics on tested strains morphology was also studied. The results show that the antibiotics have an inhibitory effect at higher concentrations (891.76 μg/mL to 1495.10 μg/mL). The highest values of MIC were estimated for Synechocystis sp. (PCC 6803) and Cephalotrix sp. (KL 18). The stimulation of growth was observed in the strains Synechocystis (PCC 6803) and Cephalothrix (KL 18). The most sensitive strains to selected antibiotics were Chlorogleopsis fritschii (CCALA 1005), Lyngbya martensiana (CCALA 930), and Geitlerinema acuminatum (CCALA 141). Strain Cyanobium sp. (LH14) was sensitive to streptomycin sulphate and gentamicin sulphate, but sulfacetamide stimulated its growth. The visible morphological changes were caused by streptomycin in Chlorogloeopsis fritschii (CCALA 1005). After five days of cultivation the bleached cells were present in the cultures of this strain.","PeriodicalId":16348,"journal":{"name":"Journal of microbiology, biotechnology and food sciences","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47233815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Fedorková Silvia, J. Musilová, Ľ. Harangozo, Marek Šnirc, Monika Ňorbová, H. Franková, Natália Čeryová
The Horehronie region belongs to the oldest industrial areas in Slovakia. It is contaminated by residual acid tar dumps from times of Petrochema Dubová factory production since 1964. In the presented study, heavy metals (Cu, Cd, Ni, Zn, Pb) were determined in soils and forest fruits (Vaccinium myrtillus L., Vaccinium vitis-idaea L., Fragaria vesca L., Rosa canina L., Rubus idaeus L.) collected over two years from five locations in the Horehronie using Flame AAS method (Zn, Cu, Ni) and Graphite Furnace AAS method (Cd, Pb). The cadmium content, which was higher than its limit value in all analysed soils, poses the biggest threat. We determined more than 20 times the limit value for cadmium in the sample Veľká Smrekovica (9.17 mg/kg).The contamination factor (Cf) values and the geoaccumulation index (Igeo) indicate that this hazardous metal is involved in soil contamination to a large extent. The highest values of the degree of contamination (Cdeg) and the pollution load index (PLI) were determined in samples from the Ždiarska dolina and the Veľká Smrekovica. The contents of nickel and zinc were higher than the limit values only in the 1st sampling year in the Ždiarska dolina sample (46.30 mg/kg; 138.30 mg/kg). The results showed that soil monitoring and remediation of old environmental burdens are important in the territory of the Horehronie region. The highest permissible amounts for cadmium were exceeded in all types of forest fruits (0.03-0.18 mg/kg FW). The contents of nickel and lead were exceeded only in the sample of rosehips (Rosa canina). The contents of zinc and copper were lower than the permissible amounts in all samples. The estimated daily intake (EDI) was lower than the acceptable daily intake of all heavy metals. The bioaccumulation factor (BAF), total hazardous quotient and hazard index of the analysed samples was < 1, which means that the consumption of forest fruits from the Horehronie does not pose a health risk for the consumer.
{"title":"THE RISK OF FOREST FRUITS CONTAMINATION BY HEAVY METALS IN THE HOREHRONIE REGION (SLOVAKIA)","authors":"Fedorková Silvia, J. Musilová, Ľ. Harangozo, Marek Šnirc, Monika Ňorbová, H. Franková, Natália Čeryová","doi":"10.55251/jmbfs.10068","DOIUrl":"https://doi.org/10.55251/jmbfs.10068","url":null,"abstract":"The Horehronie region belongs to the oldest industrial areas in Slovakia. It is contaminated by residual acid tar dumps from times of Petrochema Dubová factory production since 1964. In the presented study, heavy metals (Cu, Cd, Ni, Zn, Pb) were determined in soils and forest fruits (Vaccinium myrtillus L., Vaccinium vitis-idaea L., Fragaria vesca L., Rosa canina L., Rubus idaeus L.) collected over two years from five locations in the Horehronie using Flame AAS method (Zn, Cu, Ni) and Graphite Furnace AAS method (Cd, Pb). The cadmium content, which was higher than its limit value in all analysed soils, poses the biggest threat. We determined more than 20 times the limit value for cadmium in the sample Veľká Smrekovica (9.17 mg/kg).The contamination factor (Cf) values and the geoaccumulation index (Igeo) indicate that this hazardous metal is involved in soil contamination to a large extent. The highest values of the degree of contamination (Cdeg) and the pollution load index (PLI) were determined in samples from the Ždiarska dolina and the Veľká Smrekovica. The contents of nickel and zinc were higher than the limit values only in the 1st sampling year in the Ždiarska dolina sample (46.30 mg/kg; 138.30 mg/kg). The results showed that soil monitoring and remediation of old environmental burdens are important in the territory of the Horehronie region. The highest permissible amounts for cadmium were exceeded in all types of forest fruits (0.03-0.18 mg/kg FW). The contents of nickel and lead were exceeded only in the sample of rosehips (Rosa canina). The contents of zinc and copper were lower than the permissible amounts in all samples. The estimated daily intake (EDI) was lower than the acceptable daily intake of all heavy metals. The bioaccumulation factor (BAF), total hazardous quotient and hazard index of the analysed samples was < 1, which means that the consumption of forest fruits from the Horehronie does not pose a health risk for the consumer.","PeriodicalId":16348,"journal":{"name":"Journal of microbiology, biotechnology and food sciences","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41879714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
K. Kukurová, Z. Ciesarová, Viera Jelemenská, Martina Orolínová, Zerina Duhović, J. Kubincová, Zuzana Dubová, J. Horváthová, B. Tobolková, M. Murkovic, B. Siegmund
The quality evaluation and physicochemical parameters assessment of Slovak and Austrian monofloral honeys were performed. In total 65 local monofloral honeys denoted by beekeepers as acacia (n = 20), rapeseed (n = 10), linden (n = 10), honeydew (n = 6), forest (n = 12) and chestnut (n = 7) were collected. The parameters such as moisture content, water activity, electrical conductivity, pH, free acidity, and 5‑hydroxymethylfurfural (HMF) content were assessed for physicochemical characterization and comparison of all monofloral honeys. Electrical conductivity showed to be the most significant indicator for honeydew origin, that together with pH and aw led to 81.54 % correct classification of samples according to honey type using multivariate statistical analysis. In context to undesirable fermentation and spoilage, aw value below 0.600 showed to be an important control parameter that could be recommended to control especially for creamed rapeseed honeys.
{"title":"QUALITATIVE PARAMETERS OF MONOFLORAL HONEYS FROM SLOVAKIA AND AUSTRIA AND THE ROLE OF WATER ACTIVITY","authors":"K. Kukurová, Z. Ciesarová, Viera Jelemenská, Martina Orolínová, Zerina Duhović, J. Kubincová, Zuzana Dubová, J. Horváthová, B. Tobolková, M. Murkovic, B. Siegmund","doi":"10.55251/jmbfs.9938","DOIUrl":"https://doi.org/10.55251/jmbfs.9938","url":null,"abstract":"The quality evaluation and physicochemical parameters assessment of Slovak and Austrian monofloral honeys were performed. In total 65 local monofloral honeys denoted by beekeepers as acacia (n = 20), rapeseed (n = 10), linden (n = 10), honeydew (n = 6), forest (n = 12) and chestnut (n = 7) were collected. The parameters such as moisture content, water activity, electrical conductivity, pH, free acidity, and 5‑hydroxymethylfurfural (HMF) content were assessed for physicochemical characterization and comparison of all monofloral honeys.\u0000Electrical conductivity showed to be the most significant indicator for honeydew origin, that together with pH and aw led to 81.54 % correct classification of samples according to honey type using multivariate statistical analysis. In context to undesirable fermentation and spoilage, aw value below 0.600 showed to be an important control parameter that could be recommended to control especially for creamed rapeseed honeys.","PeriodicalId":16348,"journal":{"name":"Journal of microbiology, biotechnology and food sciences","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45596658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pooja Singh, Angkita Sharma, M. Bordoloi, Shoma Paul Nandi
Studies of potent antibiotics from plant endophytes have become a new interest among researchers. Plant derived antibiotics are costly, laborious, time-consuming and require extensive area of land. Endophytes can be used as an alternative source of antibiotics. Endophytes are microbes (bacteria and fungi) that live inside healthy plant tissues and do not show any antagonistic symptoms. They produce a variety of secondary metabolites having various commercial properties, medicinal, environmental, and agronomic purposes. In present work, endophytic fungi Aspergillus flavus associated with Argemone mexicana, was tested for its antimicrobial activity. The secondary metabolites of A. flavus were extracted using ethyl acetate solvent and tested for their antibacterial potential against certain Gram-negative bacteria, Salmonella typhimurium, Klebsiella pneumoniae, Vibrio cholerae, Escherichia coli, and Gram-positive bacteria Staphylococcus aureus using Agar well diffusion assay. Thin layer chromatographic bands were also checked further to know the bioactive spot. Gas chromatography- mass spectroscopy (GC-MS) was used to identify the bioactive compounds in TLC fraction and eighteen bioactive compounds were identified. Molecular docking and MM-GBSA were performed for the bioactive compounds with the respective proteins of the S. aureus (5JG0, 5JQ6, 1T2W, 3WYI, 3TFP, 3G7B, and 3WQU) and E. coli (1AJ0, 1GG4, 1 AJ6, 5ZHE and 7D6G) by using Schrodinger Maestro. In silico analysis suggested that Methyl (3-oxo-2-pentylcyclopentyl) acetate (MOPA) and 13-Hexyloxacyclotridec-10-en-2-one (HCT) exhibited the best binding affinity with most of the receptors. Pharmacokinetics and physicochemical analysis also showed that HCT and MOPA are the best drug candidate for the S. aureus and E. coli.
{"title":"ANTIMICROBIAL, ANTIOXIDANT, GC-MS ANALYSIS AND MOLECULAR DOCKING ANALYSIS OF BIOACTIVE COMPOUNDS OF ENDOPHYTE ASPERGILLUS FLAVUS FROM ARGEMONE MEXICANA","authors":"Pooja Singh, Angkita Sharma, M. Bordoloi, Shoma Paul Nandi","doi":"10.55251/jmbfs.9970","DOIUrl":"https://doi.org/10.55251/jmbfs.9970","url":null,"abstract":"Studies of potent antibiotics from plant endophytes have become a new interest among researchers. Plant derived antibiotics are costly, laborious, time-consuming and require extensive area of land. Endophytes can be used as an alternative source of antibiotics. Endophytes are microbes (bacteria and fungi) that live inside healthy plant tissues and do not show any antagonistic symptoms. They produce a variety of secondary metabolites having various commercial properties, medicinal, environmental, and agronomic purposes. In present work, endophytic fungi Aspergillus flavus associated with Argemone mexicana, was tested for its antimicrobial activity. The secondary metabolites of A. flavus were extracted using ethyl acetate solvent and tested for their antibacterial potential against certain Gram-negative bacteria, Salmonella typhimurium, Klebsiella pneumoniae, Vibrio cholerae, Escherichia coli, and Gram-positive bacteria Staphylococcus aureus using Agar well diffusion assay. Thin layer chromatographic bands were also checked further to know the bioactive spot. Gas chromatography- mass spectroscopy (GC-MS) was used to identify the bioactive compounds in TLC fraction and eighteen bioactive compounds were identified. Molecular docking and MM-GBSA were performed for the bioactive compounds with the respective proteins of the S. aureus (5JG0, 5JQ6, 1T2W, 3WYI, 3TFP, 3G7B, and 3WQU) and E. coli (1AJ0, 1GG4, 1 AJ6, 5ZHE and 7D6G) by using Schrodinger Maestro. In silico analysis suggested that Methyl (3-oxo-2-pentylcyclopentyl) acetate (MOPA) and 13-Hexyloxacyclotridec-10-en-2-one (HCT) exhibited the best binding affinity with most of the receptors. Pharmacokinetics and physicochemical analysis also showed that HCT and MOPA are the best drug candidate for the S. aureus and E. coli.","PeriodicalId":16348,"journal":{"name":"Journal of microbiology, biotechnology and food sciences","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46974210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Miroslav Rajninec, Marek Vidiš, M. Tomka, Mária Šedivá, A. Gažiová, J. Mucha
The global pandemic of disease COVID-19 caused by the pathogenic SARS-Cov-2 virus brought more interest in the public health community for known silver with its potential antimicrobial properties to fight infection. One of the ways to stop virus to protect community transmission is the application of nanotechnology of silver nanoparticles on the exposed surfaces of daily used materials in public, e.g., transportation, community spaces, hospitals, and everywhere where the potential infection load is increased. Published technology to coat AgNPs on surfaces differs in the preparation of nanocomposites and substrates, which results in different mechanical and antimicrobial properties. In our study, we focused on the properties of AgNPs prepared by HiTUS and PVD technology with a challenge to test the antimicrobial effect towards the model of gram-negative bacteria (Escherichia coli), fungi (Trichoderma harzianum) and related enteroviruses (Poliovirus and Coxsackie). All tested materials showed 59% or more growth inhibition of E. coli. Growth of T. harzianum was inhibited by 16% in the presence of AgTiB2 50W, and other materials caused 37% to 68% inhibition. Enteroviruses infection was completely inhibited after 1 hour of AgNPs treatment. Only Coxsackie A7 retained infection capability after 30 minutes of treatment with AgNPs. Moreover, the ICP-OES-measured amounts of silver released in cultivation media are lower than most published studies of silver nanoparticles with a comparable antimicrobial effect. Keeping silver concentration at the lowest possible limit is one of the most critical factors for producing environmentally safe antimicrobial materials for everyday use.
{"title":"ANTIMICROBIAL EFFECT OF SILVER NANOPARTICLE-BASED THIN FILMS","authors":"Miroslav Rajninec, Marek Vidiš, M. Tomka, Mária Šedivá, A. Gažiová, J. Mucha","doi":"10.55251/jmbfs.10073","DOIUrl":"https://doi.org/10.55251/jmbfs.10073","url":null,"abstract":"The global pandemic of disease COVID-19 caused by the pathogenic SARS-Cov-2 virus brought more interest in the public health community for known silver with its potential antimicrobial properties to fight infection. One of the ways to stop virus to protect community transmission is the application of nanotechnology of silver nanoparticles on the exposed surfaces of daily used materials in public, e.g., transportation, community spaces, hospitals, and everywhere where the potential infection load is increased. Published technology to coat AgNPs on surfaces differs in the preparation of nanocomposites and substrates, which results in different mechanical and antimicrobial properties. In our study, we focused on the properties of AgNPs prepared by HiTUS and PVD technology with a challenge to test the antimicrobial effect towards the model of gram-negative bacteria (Escherichia coli), fungi (Trichoderma harzianum) and related enteroviruses (Poliovirus and Coxsackie). All tested materials showed 59% or more growth inhibition of E. coli. Growth of T. harzianum was inhibited by 16% in the presence of AgTiB2 50W, and other materials caused 37% to 68% inhibition. Enteroviruses infection was completely inhibited after 1 hour of AgNPs treatment. Only Coxsackie A7 retained infection capability after 30 minutes of treatment with AgNPs. Moreover, the ICP-OES-measured amounts of silver released in cultivation media are lower than most published studies of silver nanoparticles with a comparable antimicrobial effect. Keeping silver concentration at the lowest possible limit is one of the most critical factors for producing environmentally safe antimicrobial materials for everyday use.","PeriodicalId":16348,"journal":{"name":"Journal of microbiology, biotechnology and food sciences","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45150407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
W. E. Putra, Sustiprijatno, Arief Hidayatullah, D. Widiastuti, Muhammad F Heikal, W. Salma
Glucose toxicity become serious risk factor of type 2 diabetes mellitus. Multiple complication can be caused of the abnormal condition of glucose level in blood. Thus, managing the plasma glucose is one of the strategy to minimize the negative effects of type 2 diabetes mellitus. In this present study, we aimed to virtually evaluate the bioactive compounds from Alpinia galanga as inhibitor agent against alpha-glucosidase, which was known as carbohydrate catabolic enzyme. The 2D structure of ligands were retrieved from PubChem database (pubchem.ncbi.nlm.nih.gov/) and were evaluated based on Lipinski rule of five for ensured the druglike-ness standard, while the sequence and 3D structure of target protein, alpha-glucosidase, was retrieved from UniProt database (https://www.uniprot.org/) and was modelled through SWISS-MODEL (swissmodel.expasy.org/). All ligands used in this study then underwent optimization prior to molecular docking and analyzing procedures. According to our prediction results, we found that galangin and 1's-1'-acetoxychavicol acetate which known as bioactive compounds found in A. galanga have significant potency as inhibitor agents against the alpha-glucosidase due to its binding affinity scores and other physicochemical properties compared to the anti-diabetic drug, mignitol. Thus, from this finding, we have recent starting point to expand the potency of these bioactive compounds, especially for inhibiting the alpha-glucosidase as the novel strategy to reduce the poor prognosis of type 2 diabetes mellitus.
{"title":"VIRTUAL SCREENING OF NATURAL ALPHA-GLUCOSIDASE INHIBITOR FROM ALPINIA GALANGA BIOACTIVE COMPOUNDS AS ANTI-DIABETIC CANDIDATE","authors":"W. E. Putra, Sustiprijatno, Arief Hidayatullah, D. Widiastuti, Muhammad F Heikal, W. Salma","doi":"10.55251/jmbfs.4353","DOIUrl":"https://doi.org/10.55251/jmbfs.4353","url":null,"abstract":"Glucose toxicity become serious risk factor of type 2 diabetes mellitus. Multiple complication can be caused of the abnormal condition of glucose level in blood. Thus, managing the plasma glucose is one of the strategy to minimize the negative effects of type 2 diabetes mellitus. In this present study, we aimed to virtually evaluate the bioactive compounds from Alpinia galanga as inhibitor agent against alpha-glucosidase, which was known as carbohydrate catabolic enzyme. The 2D structure of ligands were retrieved from PubChem database (pubchem.ncbi.nlm.nih.gov/) and were evaluated based on Lipinski rule of five for ensured the druglike-ness standard, while the sequence and 3D structure of target protein, alpha-glucosidase, was retrieved from UniProt database (https://www.uniprot.org/) and was modelled through SWISS-MODEL (swissmodel.expasy.org/). All ligands used in this study then underwent optimization prior to molecular docking and analyzing procedures. According to our prediction results, we found that galangin and 1's-1'-acetoxychavicol acetate which known as bioactive compounds found in A. galanga have significant potency as inhibitor agents against the alpha-glucosidase due to its binding affinity scores and other physicochemical properties compared to the anti-diabetic drug, mignitol. Thus, from this finding, we have recent starting point to expand the potency of these bioactive compounds, especially for inhibiting the alpha-glucosidase as the novel strategy to reduce the poor prognosis of type 2 diabetes mellitus.","PeriodicalId":16348,"journal":{"name":"Journal of microbiology, biotechnology and food sciences","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48950434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The purpose of this study was to evaluate the cytotoxic and antioxidant properties of a hydromethanolic extract of Rivina humilis L fruits extract (HMERH) on human cancer cell lines and various in vitro models. HMERH concentrations ranging from 5 to 400mg/ml were utilized in several assay methods such as hydroxyl radicals, DPPH, superoxide radical scavenging activity, metal chelating, and nitric oxide radical test, ABTS, and reducing power assay. All antioxidant assays employed ascorbic acid as the standard. Its short-term cytotoxicity on EAC and DLA cells was determined using the trypan blue dye exclusion method, as well as the lactate dehydrogenase (LDH) leakage assay and in vitro cytotoxicity on MEF-L929, DU-145, and PC-3 cells using the MTT assay. The total antioxidant potential was also determined using the phosphomolybdenum test. HMERH demonstrated considerable cytotoxicity against all cancer cell lines examined. The findings of the free radical scavenging activities demonstrated a concentration-dependent and antiradical activity caused by the reduction of ABTS, DPPH, NO, OH, and SO radicals to non-radical form. The findings reveal that antioxidants and cytotoxic agents have potent antioxidant and cytotoxic effects on cancer cell lines. Hence, this fruit extract has the potential to be a natural antioxidant and anticancer drug source, which could be valuable in the development of new anticancer therapies.
{"title":"IN VITRO ANTIOXIDANT AND ANTICANCER ACTIVITY OF THE FRUIT EXTRACT OF RIVINA HUMILIS L. (RED PIGEON BERRY)","authors":"A. Raju, Dinu George","doi":"10.55251/jmbfs.9347","DOIUrl":"https://doi.org/10.55251/jmbfs.9347","url":null,"abstract":"The purpose of this study was to evaluate the cytotoxic and antioxidant properties of a hydromethanolic extract of Rivina humilis L fruits extract (HMERH) on human cancer cell lines and various in vitro models. HMERH concentrations ranging from 5 to 400mg/ml were utilized in several assay methods such as hydroxyl radicals, DPPH, superoxide radical scavenging activity, metal chelating, and nitric oxide radical test, ABTS, and reducing power assay. All antioxidant assays employed ascorbic acid as the standard. Its short-term cytotoxicity on EAC and DLA cells was determined using the trypan blue dye exclusion method, as well as the lactate dehydrogenase (LDH) leakage assay and in vitro cytotoxicity on MEF-L929, DU-145, and PC-3 cells using the MTT assay. The total antioxidant potential was also determined using the phosphomolybdenum test. HMERH demonstrated considerable cytotoxicity against all cancer cell lines examined. The findings of the free radical scavenging activities demonstrated a concentration-dependent and antiradical activity caused by the reduction of ABTS, DPPH, NO, OH, and SO radicals to non-radical form. The findings reveal that antioxidants and cytotoxic agents have potent antioxidant and cytotoxic effects on cancer cell lines. Hence, this fruit extract has the potential to be a natural antioxidant and anticancer drug source, which could be valuable in the development of new anticancer therapies.","PeriodicalId":16348,"journal":{"name":"Journal of microbiology, biotechnology and food sciences","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43405701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
With an increasing concern by health conscious people search for more nutritious pasta products rich in minerals, phenolic compounds and dietary low glycemic index and have become the subject of primary significance. In this work effect of the addition of leaf powder as well as leaf extracted powder of Clerodendrum glandulosum L. on properties of pasta was studied. Functional pasta was developed by using a blend of durum wheat semolina, leaf powder and extracted leaf powder. The plant powder and extracts powder were added in the formulation with four proportions (2.5%, 5.0%, 7.5% and 10%) respectively. The obtained products were analyzed for their cooking attributes, textural properties, colour, phytochemical properties and sensory characteristics. Incorporation of leaf powder and extracts remarkably elevated the antioxidant activity (42.01 ± 1.40 to 74.19 ± 2.58%) and total phenolic content (13.96 ± 1.84 to 43.55 ± 2.55 mg GAE/ g). The moisture content decreases (55.79 ± 1.53 to 48.58 ± 1.51%) with increase in leaf or leaf extract powder. The swelling index and water absorption index of functional pasta were recorded lower values than the control pasta. With the addition of more leaf and extract powder, the product's lightness (L*) and yellowness (b*) reduced while its redness (a*) grew. The hardness, springiness and chewiness were found to be relatively lower than that of the Control. The findings of the current study showed that among the pastas that have been fortified, the pasta with Clerodendrum glandulosum L. additions up to E-2.5% had the highest sensory acceptance. Good marketing expectations are given because it supplied sensory acceptance similar to the standard product.
{"title":"CLERODENDRUM GLANDULOSUM L. INCORPORATED FUNCTIONAL PASTA: PHYTOCHEMICAL, TEXTUAL, STRUCTURAL AND SENSORY STUDIES","authors":"Nemnunhoi Haokip, R. Duary, L. Badwaik","doi":"10.55251/jmbfs.9599","DOIUrl":"https://doi.org/10.55251/jmbfs.9599","url":null,"abstract":"With an increasing concern by health conscious people search for more nutritious pasta products rich in minerals, phenolic compounds and dietary low glycemic index and have become the subject of primary significance. In this work effect of the addition of leaf powder as well as leaf extracted powder of Clerodendrum glandulosum L. on properties of pasta was studied. Functional pasta was developed by using a blend of durum wheat semolina, leaf powder and extracted leaf powder. The plant powder and extracts powder were added in the formulation with four proportions (2.5%, 5.0%, 7.5% and 10%) respectively. The obtained products were analyzed for their cooking attributes, textural properties, colour, phytochemical properties and sensory characteristics. Incorporation of leaf powder and extracts remarkably elevated the antioxidant activity (42.01 ± 1.40 to 74.19 ± 2.58%) and total phenolic content (13.96 ± 1.84 to 43.55 ± 2.55 mg GAE/ g). The moisture content decreases (55.79 ± 1.53 to 48.58 ± 1.51%) with increase in leaf or leaf extract powder. The swelling index and water absorption index of functional pasta were recorded lower values than the control pasta. With the addition of more leaf and extract powder, the product's lightness (L*) and yellowness (b*) reduced while its redness (a*) grew. The hardness, springiness and chewiness were found to be relatively lower than that of the Control. The findings of the current study showed that among the pastas that have been fortified, the pasta with Clerodendrum glandulosum L. additions up to E-2.5% had the highest sensory acceptance. Good marketing expectations are given because it supplied sensory acceptance similar to the standard product.","PeriodicalId":16348,"journal":{"name":"Journal of microbiology, biotechnology and food sciences","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45414061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
K. Allali, Miyada Zamoum, Abderrahmane Benadjila, A. Zitouni, Yacine Goudjal
This study focused on the production of the phytohormone indole-3-acetic acid (IAA) using tomato plant tissues as feedstock and on highlighting its growth promotion effect on tomato seedlings. Twelve actinobacterial strains were screened for IAA production under standard growth conditions and the strain Streptomyces plicatus PT2 was selected as a promising producer. The establishment of growth conditions to increase IAA production by S. plicatus PT2 was conducted using Plackett-Burman mathematical design with seven variables. The variables L-tryptophan quantity and tomato roots-extract rate were the two significant variables influencing IAA production. The most impacting variables were optimized using the surface methodology (RSM) formulated according to the Central Composite Design (CCD). The optimized broth supplemented with 4 mg L-1 of L-tryptophan and 25% of tomato roots-extract significantly improved the IAA production from 96.3 µg mL-1 within 120h to a maximum of 110.29 µg mL-1 within 96h. The in-planta application of the produced IAA and a chemical IAA showed a significant increase in the dry weight, the shoot and root lengths of tomato seedlings. This is the first study showing the production of IAA using Streptomyces plicatus strain PT2 by exploring tomato plant tissues and its plant-growth promotion efficacy compared to a chemical marketed one. These results support a reasonable approach for the valorization of abundant worldwide tomato plant wastes in the phytohormones production bioprocesses.
{"title":"VALORIZATION OF TOMATO PLANT WASTES AND OPTIMIZATION OF GROWTH CONDITIONS FOR INDOLE-3-ACETIC ACID PRODUCTION BY Streptomyces plicatus STRAIN PT2","authors":"K. Allali, Miyada Zamoum, Abderrahmane Benadjila, A. Zitouni, Yacine Goudjal","doi":"10.55251/jmbfs.9580","DOIUrl":"https://doi.org/10.55251/jmbfs.9580","url":null,"abstract":"This study focused on the production of the phytohormone indole-3-acetic acid (IAA) using tomato plant tissues as feedstock and on highlighting its growth promotion effect on tomato seedlings. Twelve actinobacterial strains were screened for IAA production under standard growth conditions and the strain Streptomyces plicatus PT2 was selected as a promising producer. The establishment of growth conditions to increase IAA production by S. plicatus PT2 was conducted using Plackett-Burman mathematical design with seven variables. The variables L-tryptophan quantity and tomato roots-extract rate were the two significant variables influencing IAA production. The most impacting variables were optimized using the surface methodology (RSM) formulated according to the Central Composite Design (CCD). The optimized broth supplemented with 4 mg L-1 of L-tryptophan and 25% of tomato roots-extract significantly improved the IAA production from 96.3 µg mL-1 within 120h to a maximum of 110.29 µg mL-1 within 96h. The in-planta application of the produced IAA and a chemical IAA showed a significant increase in the dry weight, the shoot and root lengths of tomato seedlings. This is the first study showing the production of IAA using Streptomyces plicatus strain PT2 by exploring tomato plant tissues and its plant-growth promotion efficacy compared to a chemical marketed one. These results support a reasonable approach for the valorization of abundant worldwide tomato plant wastes in the phytohormones production bioprocesses.","PeriodicalId":16348,"journal":{"name":"Journal of microbiology, biotechnology and food sciences","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42072291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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