Journal of plant physiology最新文献_第9页

Insights into responses to elevated temperatures in Solanum tuberosum cultivars with contrasting sensitivity 洞察对高温的响应在龙葵品种与对比敏感性

IF 4.1 3区生物学 Q1 PLANT SCIENCES

Journal of plant physiology

Pub Date : 2025-12-23 DOI: 10.1016/j.jplph.2025.154683

Selina Beck , Luisa Höfner , David Rüscher , Danuše Tarkowská , Jitka Široká , Stephen Reid , David Pscheidt , Jörg Hofmann , Ondřej Novák , Miroslav Strnad , Sophia Sonnewald

Elevated temperatures caused by climate change threaten potato production. To understand heat stress adaptations and variety-specific responses, plants of a susceptible (Cecile) and a tolerant cultivar (Solara) were exposed to elevated temperatures (30/28 °C) for 21 days at tuberization stage. Phenotypic, physiological, transcriptional and metabolic changes were analyzed in comparison to ambient temperatures (21/19 °C). Heat stress caused shoot elongation and tuber weight loss, which were more pronounced in Cecile. Transcriptome analysis of leaf samples revealed a stronger decrease of photosynthesis-associated genes in the sensitive cultivar Cecile, which was associated with decreased chlorophyll fluorescence and an early senescence. These effects correlated with strongly elevated levels of salicylic acid and ethylene. In contrast, Solara showed delayed senescence and a higher expression of sugar and amino acid transporters suggesting an adaptive mechanism to maintain carbohydrate and amino acid allocation. The expression of known tuberization regulators including SP6A, exhibited a similar response to heat in both varieties, with decreasing expression of SP6A. Solara exhibited a constitutively higher expression of PEBP14/15 and MADS13, which potentially promote tuberization and may support tuber growth under heat. Regardless of variety, a few genes, such as HSP20 and HSP70, were induced by heat and may serve as heat stress marker genes. Altogether, the results indicate that delayed senescence, stable photosynthesis, efficient assimilate translocation, and differential regulation of tuberization pathways contribute to heat tolerance in Solara. These insights improve our understanding of the molecular basis of heat resilience and provide potential targets for breeding climate-resilient potato varieties.

气候变化导致的气温升高威胁着马铃薯的生产。为了了解热胁迫的适应性和品种特异性反应，将一个敏感品种（Cecile）和一个耐受性品种（Solara）的植株在结核期暴露在高温（30/28°C）下21天。与环境温度（21/19°C）相比，分析了表型、生理、转录和代谢变化。热胁迫导致了茎伸长和块茎重量下降，其中在茜草中表现得更为明显。叶片样本转录组分析显示，敏感品种茜草的光合作用相关基因明显减少，这与叶绿素荧光下降和早期衰老有关。这些影响与水杨酸和乙烯水平的升高密切相关。相比之下，Solara表现出延迟衰老和糖和氨基酸转运蛋白的更高表达，这表明一种维持碳水化合物和氨基酸分配的适应性机制。在两个品种中，包括SP6A在内的已知结核调节因子的表达对高温的响应相似，但SP6A的表达量有所下降。Solara表现出PEBP14/15和MADS13的高表达，这可能促进了块茎的形成，并可能支持块茎在高温下的生长。无论品种如何，少数基因如HSP20和HSP70被热诱导，可能作为热应激标记基因。综上所述，延缓衰老、稳定的光合作用、高效的同化物转运和不同调控的结核途径对茄的耐热性有重要影响。这些见解提高了我们对耐热性分子基础的理解，并为培育气候适应型马铃薯品种提供了潜在的目标。

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引用次数: 0

MsTIFY10a gene from alfalfa negatively regulates drought and salt tolerance in transgenic tobacco 苜蓿MsTIFY10a基因负调控转基因烟草的耐旱性和耐盐性

IF 4.1 3区生物学 Q1 PLANT SCIENCES

Journal of plant physiology

Pub Date : 2025-12-22 DOI: 10.1016/j.jplph.2025.154684

Qi Chen, Yan Zhang, Ying Tian, Jing Xu, Qing-Wen Fu, Zhen-Yi Li, Feng-Ling Shi, Cui-Ping Gao, Zhi-Qiang Zhang

The JAZ protein family acts as a key negative regulator in the jasmonic acid signaling pathway, interacting with transcription factors and playing essential roles in plant growth, development, and abiotic stress responses. However, the specific function of JAZ transcription factors in mediating salt and drought stress tolerance in alfalfa (Medicago sativa) remains unclear. In this study, we cloned MsTIFY10a, a JAZ gene from alfalfa, and found that its expression was downregulated under salt and drought stresses. Heterologous expression of MsTIFY10a in tobacco significantly reduced tolerance to both drought and salt stresses in seedlings and mature plants. Physiological analysis revealed that MsTIFY10a overexpression suppressed the antioxidant system, including superoxide dismutase (SOD) and peroxidase (POD), compromised photosynthetic capacity, and exacerbated membrane damage, collectively leading to reduced stress tolerance. Moreover, under drought or salt treatment, MsTIFY10a overexpression downregulated the expression of several reactive oxygen species (ROS)-related and stress-responsive genes. In summary, MsTIFY10a may functions as a negative regulator in abiotic stress responses, providing a basis for further investigation into its mechanistic roles in alfalfa.

JAZ蛋白家族是茉莉酸信号通路的关键负调控蛋白，与转录因子相互作用，在植物生长发育和非生物胁迫响应中发挥重要作用。然而，JAZ转录因子在苜蓿耐盐和干旱胁迫中的具体作用尚不清楚。本研究克隆了苜蓿JAZ基因MsTIFY10a，发现该基因在盐胁迫和干旱胁迫下表达下调。MsTIFY10a在烟草中的异源表达显著降低了烟草幼苗和成熟植株对干旱和盐胁迫的耐受性。生理分析表明，MsTIFY10a过表达抑制抗氧化系统，包括超氧化物歧化酶（SOD）和过氧化物酶（POD），降低光合能力，加剧膜损伤，共同导致胁迫耐受性降低。此外，在干旱或盐处理下，MsTIFY10a过表达下调了几种活性氧（ROS）相关基因和应激反应基因的表达。综上所述，MsTIFY10a可能在非生物胁迫响应中发挥负调控作用，为进一步研究其在苜蓿中的机制作用提供了基础。

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引用次数: 0

Increasing CO2 concentration promoted the biomass accumulation but decreased the mineral nutrition and forage quality of Leymus chinensis CO2浓度的增加促进了羊草生物量的积累，但降低了羊草的矿质营养和饲料品质。

IF 4.1 3区生物学 Q1 PLANT SCIENCES

Journal of plant physiology

Pub Date : 2025-12-19 DOI: 10.1016/j.jplph.2025.154680

Jie Yu , Cheng Ji , Yongwei Sun , Zhi Qi

The global atmospheric CO₂ concentration is predicted to increase from the current approximate 450 to 700 ppm by end of this century. To evaluate its potential impact on sustainability of grassland, Leymus chinensis, the dominant wild forage species in the eastern Eurasian Steppe, was cultivated in two growth chambers for a month with ambient 450 ppm (aCO₂) as the control and elevated 700 ppm CO₂ (eCO₂) as simulation of the future respectively. The eCO₂ increased the aboveground biomass, net photosynthesis rate and contents of carbohydrates, as well as the Mn contents in the leaves. The eCO₂ decreased the stomatal conductance, transpiration rate and the contents of mineral elements S, P, K, Fe, Zn in the xylem sap and leaves, as well as expression of ion transporter-encoding transcripts. As response to the eCO₂, the DNA, RNA and protein metabolism related transcripts were over-represented in the down-regulation transcriptome, accompanied with reduction in the contents of amino acids. The eCO₂ significantly suppressed expression of lipid metabolism-encoding transcripts and contents of phospholipids, as well as expression of vesicle-traffic encoding transcripts. The eCO₂ decreased contents of bioactive compounds flavonoids, terpenoids including gibberellins and steroids. These data imply that the predicted increasing atmospheric CO₂ concentration in the near future would have negative impacts on the forage quality of the grass.

预计到本世纪末，全球大气中的二氧化碳浓度将从目前的大约450 ppm增加到700 ppm。为了评估其对草地可持续性的潜在影响，以欧亚东部草原的优势野生牧草羊草（Leymus chinensis）为研究对象，在两个生长室内分别以450 ppm （aCO2）环境为对照和700 ppm （eCO2）升高为模拟未来，培养一个月。eCO2增加了地上生物量、净光合速率、碳水化合物含量和叶片中Mn含量。eCO2降低了木质部液和叶片中气孔导度、蒸腾速率和矿质元素S、P、K、Fe、Zn的含量，降低了离子转运蛋白编码转录物的表达。作为对eCO2的响应，DNA、RNA和蛋白质代谢相关转录本在下调转录组中被过度表达，同时氨基酸含量减少。eCO2显著抑制脂质代谢编码转录物的表达和磷脂含量，以及囊泡运输编码转录物的表达。eCO2降低了黄酮类化合物、萜类化合物（包括赤霉素和类固醇）的含量。这些数据表明，在不久的将来，预测的大气CO2浓度增加将对牧草的饲料质量产生负面影响。

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引用次数: 0

The adaptation strategy of Astragalus mongholicus shoots to the root Fe2+ deficiency and its strong stimulating effect on glucoliquiritin apioside accumulation 黄芪茎部对根系Fe2+缺乏的适应策略及其对糖醛酸苷苷积累的强烈刺激作用

IF 4.1 3区生物学 Q1 PLANT SCIENCES

Journal of plant physiology

Pub Date : 2025-12-19 DOI: 10.1016/j.jplph.2025.154679

Yanbing Dong , Yang Nan , Zhi Qi

Iron is an essential micronutrient. However, nearly 40 % of arable land worldwide suffers from iron deficiency. In this study, the adaptation of shoots to root Fe²⁺ deficiency was investigated using hydroponically grown Astragalus mongholicus, a widely cultivated medicinal plant. The root Fe²⁺ deficiency significantly inhibited the plants' growth and the contents of Fe, Mo, as well as 10 metabolites, including -isorhamnetin-3-O-glucoside, and nepitrin, which have reported anti-inflammatory activities. The root Fe²⁺ deficiency promoted the contents of Ca, Mg, K, Zn, Mn, and 12 metabolites, among which the glucoliquiritin apioside, an anti-infection flavonoid, strikingly increased by 2480.6 times. Transcriptome analysis revealed that Fe²⁺ deficiency could impair cellular energy metabolism by inhibiting the expression of ATPase and other essential enzymes for the tricarboxylic acid cycle. The plants adapted to the stress by enhancing the expression of transcripts encoding V-type H⁺-ATPases, Ca²⁺ and Mg²⁺ transporting ATPases, Fe²⁺ storage protein ferritins, as well as receptor like kinase and phytohormone-related transcription factors. In the transcriptome, a transcript encoding a functional Fe²⁺ passive transporter was identified by complementing Fe²⁺/Zn²⁺ uptake defective yeast mutants. The adaptation strategy of Astragalus mongholicus to the Fe²⁺ deficiency and the potential for increasing glucoliquiritin apioside contents in the shoots by occasionally applying Fe²⁺ chelators to the cultivating soils were discussed.

铁是一种必需的微量营养素。然而，全世界近40%的可耕地缺铁。以水培栽培的蒙古黄芪（Astragalus mongholicus）为研究对象，研究了其茎部对根系Fe2+缺乏的适应性。根系Fe2+缺乏显著抑制植株生长和铁、钼含量，以及-异鼠李素-3- o -葡萄糖苷、nepitrin等10种代谢产物的抗炎活性。根系缺铁促进了钙、镁、钾、锌、锰含量和12种代谢物的含量，其中抗感染类黄酮糖醛酸苷含量显著增加了2480.6倍。转录组分析显示，缺铁可能通过抑制atp酶和其他三羧酸循环必需酶的表达而损害细胞能量代谢。植物通过增强编码v型H+- atp酶、Ca2+和Mg2+转运atp酶、Fe2+储存蛋白铁蛋白以及受体激酶和植物激素相关转录因子的表达来适应逆境。在转录组中，通过补充Fe2+/Zn2+摄取缺陷酵母突变体，鉴定了一个编码功能性Fe2+被动转运蛋白的转录本。探讨了蒙古黄芪对Fe2+缺乏的适应策略，以及间或施用Fe2+螯合剂提高栽培土壤中糖醛酸苷含量的潜力。

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引用次数: 0

Light signal transduction networks regulating phenylpropanoid, terpenoid and alkaloid biosynthesis in horticultural plants 园艺植物中调节苯丙类、萜类和生物碱生物合成的光信号转导网络

IF 4.1 3区生物学 Q1 PLANT SCIENCES

Journal of plant physiology

Pub Date : 2025-12-18 DOI: 10.1016/j.jplph.2025.154681

Yadi Chen, Lanxi Shi, Qingtao Xu, Chi Zhang, Li Wang, Weixing Li

Plant secondary metabolites (PSMs), crucial for horticultural crop quality and value, are synthesized in an organ-specific manner and are highly regulated by light. Acting beyond a mere energy source for photosynthesis, light signals are detected by specialized photoreceptors (e.g., phytochromes, cryptochromes, UV RESISTANCE LOCUS 8), triggering signaling cascades that converge on central regulators including the COP1-SPA complex and the transcription factor HY5. These regulators interact with a broad network of transcription factors, such as MYBs, bHLHs, BBXs, and PIFs, as well as epigenetic modifications, to precisely direct the transcriptional programs governing phenylpropanoid, terpenoid, and alkaloid metabolism. This review synthesizes these molecular mechanisms and discusses their implications for designing precise lighting strategies to enhance the quality and value of horticultural products in controlled-environment agriculture, thereby providing a theoretical foundation for light-quality regulation.

植物次生代谢物（psm）是一种对园艺作物品质和价值至关重要的物质，其合成具有器官特异性，并受光照高度调控。光信号不仅仅是光合作用的能量来源，还被专门的光感受器（如光敏色素、隐色素、抗紫外线位点8）检测到，触发信号级联，汇聚到包括COP1-SPA复合物和转录因子HY5在内的中心调节因子上。这些调节因子与广泛的转录因子网络相互作用，如myb、bHLHs、bbx和pif，以及表观遗传修饰，以精确指导控制苯丙素、萜类和生物碱代谢的转录程序。本文综述了这些分子机制，并讨论了它们对设计精确照明策略以提高受控环境农业园艺产品的质量和价值的意义，从而为光质量调控提供理论基础。

引用次数: 0

TaGSr-4D orchestrates lateral root development and tolerance to low nitrogen stress in Arabidopsis TaGSr-4D调控拟南芥侧根发育和对低氮胁迫的耐受。

IF 4.1 3区生物学 Q1 PLANT SCIENCES

Journal of plant physiology

Pub Date : 2025-12-18 DOI: 10.1016/j.jplph.2025.154682

Huiqiang Li , Duheng Zhang , Xi Zhang , Furong Nai , Lulu Wang , Yihao Wei , Xiaochun Wang

Lateral roots are significant for capturing nutrients and water from the soil due to their capacity to expand the uptake area of the root system. Comprehending the molecular mechanisms that regulate lateral root development would be beneficial for optimizing the root system architecture (RSA) and improving crop yield. The enzyme GS (Glutamine synthetase) is a key enzyme that assimilates ammonium into glutamine. Previous study showed that TaGSr (Triticum aestivum L. ROOT GLUTAMINE SYNTHETASE) was mainly expressed in the root. However, little is known about the function of TaGSr in root system development in wheat. In this study, we showed that TaGSr-4D was expressed at all eight developmental stages of lateral root primordia and the heterologous expression of TaGSr-4D gene from wheat promoted the lateral root development in Arabidopsis. Overexpression of TaGSr-4D increased glutamine content and auxin content in root. Moreover, qRT-PCR analysis demonstrated that the expression of IAA14, LBD18, ARF6, ARF8, YUC3, YUC5, YUC6, and YUC9 were up-regulated in TaGSr-4D-OE Arabidopsis plants compared with wild-type. The absence of lateral roots in the arf7 arf19 mutant was not complemented by TaGSr-4D overexpression. These findings suggested that TaGSr-4D-regulated lateral root development is dependent on auxin signaling pathway. Furthermore, the shoot fresh weight of overexpression of TaGSr-4D OE-1 in Arabidopsis was greatly increased (39.29 %) compared with wild-type under low nitrogen conditions. This study may provides important clues for improving RSA and yield in wheat.

侧根对从土壤中捕获养分和水分具有重要意义，因为它们具有扩大根系吸收面积的能力。了解侧根发育的分子机制有助于优化根系结构，提高作物产量。谷氨酰胺合成酶（GS）是将氨同化为谷氨酰胺的关键酶。已有研究表明，Triticum aestivum L. ROOT GLUTAMINE SYNTHETASE （TaGSr）主要在根中表达。然而，对TaGSr在小麦根系发育中的作用知之甚少。在本研究中，我们发现TaGSr-4D基因在侧根原基的8个发育阶段均有表达，并且从小麦中外源表达TaGSr-4D基因促进了拟南芥侧根的发育。过表达TaGSr-4D增加了根中谷氨酰胺含量和生长素含量。qRT-PCR分析显示，与野生型相比，TaGSr-4D-OE拟南芥中IAA14、LBD18、ARF6、ARF8、YUC3、YUC5、YUC6和YUC9的表达上调。在arf7中，arf19突变体中侧根的缺失并没有被TaGSr-4D过表达所补充。这些发现表明，tagsr - 4d调控侧根发育依赖于生长素信号通路。低氮条件下，过表达TaGSr-4D OE-1的拟南芥茎鲜重较野生型显著增加（39.29%）。该研究可能为提高小麦的RSA和产量提供重要线索。

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引用次数: 0

Detection and mapping of gm13, a QTL governing recessive resistance to rice gall midge 水稻瘿蚊隐性抗性QTL gm13的检测与定位

IF 4.1 3区生物学 Q1 PLANT SCIENCES

Journal of plant physiology

Pub Date : 2025-12-15 DOI: 10.1016/j.jplph.2025.154678

Fugang Huang , Chunlan Teng , Huayu Huang , Haiyan Cheng , Guihua Zhou , Ting Liu , Haojiang Zhu , Zhe Jiang , Shahzad Ahmad , Piqing Liu , Yongfu Qiu

The Asian rice gall midge (RGM, Orseolia oryzae Wood-Mason) is a major devastating insect pest of rice, causing continuous damage from seedling to tillering stage. Its larvae invade the basal meristematic tissues of rice shoots, secreting effectors that induce the formation of characteristic hollow, tube-like structures known as ‘silver-shoot’, which inhibits panicle development. Deploying resistant cultivars harboring RGM resistance genes remains the most effective, environment-friendly, and sustainable management strategy, yet the discovery of novel resistance loci remains critical. We found that rice variety NY74 employs a combination of antixenotic and antibiotic defenses against RGM, without a hypersensitive response during the first 16 days of infestation. Genetic segregation analysis revealed that resistance in NY74 is governed by a single recessive locus, designated as gm13. Initial mapping using bulked segregant analysis (BSA) localized gm13 to chromosome 8L. The identified quantitative trait locus (QTL) individually explained 41.7 % of the phenotypic variation, with likelihood of odd (LOD) score 14.3. Subsequently, high-resolution linkage analysis segregating progenies further refined the locus to an 82 kb interval between 18.33 Mb and 18.41 Mb. Functional annotation of the candidate region identified a resistance gene homolog, gene1, as the most promising candidate gene, characterized by a leucine-rich repeat domain. Both the gene location and recessive genetic mode distinguish gm13 from other RGM resistance locus. Our findings provide a valuable genetic resource for breeding programs and advance the molecular understanding of rice immunity against gall midge.

亚洲稻瘿蚊（Orseolia oryzae Wood-Mason）是水稻的主要破坏性害虫，从苗期到分蘖期都对水稻造成持续危害。它的幼虫侵入水稻芽的基部分生组织，分泌效应物，诱导形成典型的空心管状结构，称为“银芽”，抑制穗发育。培育含有RGM抗性基因的抗性品种仍然是最有效、最环保、最可持续的管理策略，但发现新的抗性位点仍然至关重要。我们发现，水稻品种NY74采用抗异种和抗生素防御RGM的组合，在侵染的前16天没有过敏反应。遗传分离分析表明，NY74的抗性受单个隐性位点gm13控制。利用散装分离分析（BSA）将gm13定位到染色体8L上。所鉴定的数量性状位点（QTL）单独解释了41.7%的表型变异，奇数似然（LOD）得分为14.3。随后，高分辨率连锁分析分离后代进一步将位点细化到18.33 Mb和18.41 Mb之间的82 kb区间。候选区域的功能注释鉴定出抗性基因同源基因基因1是最有希望的候选基因，其特征是一个富含亮氨酸的重复结构域。基因定位和隐性遗传模式将gm13与其他RGM抗性位点区分开来。本研究结果为水稻的遗传育种提供了宝贵的遗传资源，并促进了水稻对瘿蚊免疫的分子认识。

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引用次数: 0

The JmjC domain-containing histone demethylase ZmJMJ703 orchestrates salt stress adaptation in maize 含有JmjC结构域的组蛋白去甲基酶ZmJMJ703调控玉米的盐胁迫适应

IF 4.1 3区生物学 Q1 PLANT SCIENCES

Journal of plant physiology

Pub Date : 2025-12-15 DOI: 10.1016/j.jplph.2025.154677

Shu Wang , Lijun Jiang , Tingting Zhai , Ke Qu , Xingyu Liu , Zhaomeng Di , Yingshan Chen , Xiaoduo Lu , Xiang Li , Jiedao Zhang , Shuxin Zhang , Wei Yang

The JmjC domain-containing protein family (JMJs) represents a family of key demethylases critical for epigenetic regulation and orchestrating plant growth and developmental processes. Despite their established roles, functional investigations into JMJ proteins under abiotic stress conditions remain limited in maize. In this study, we identified and functionally characterized ZmJMJ703, a JmjC domain-containing gene exhibiting salt stress-responsive expression patterns in maize. Physiological and phenotypic analysis revealed that ZmJMJ703 mutation significantly impairs salt stress tolerance in maize seedlings. Transcriptomic profiling uncovered differential expression patterns between zmjmj703 mutants and wild-type plants, with affected genes predominantly associated with intracellular protein trafficking, amino acid metabolism, and small molecule reprogramming. Parallel proteomic analysis through mass spectrometry further demonstrated that differential protein accumulation in mutants primarily enriched pathways related to secondary metabolite biosynthesis. These integrated omics analyses collectively suggest that ZmJMJ703 may modulate metabolic pathways critical for abiotic stress responses. Functional validation was reinforced by phenotypic evaluation of Arabidopsis lines heterologous overexpressing ZmJMJ703, which exhibited enhanced salt stress tolerance compared to control plants. Collectively, these findings significantly advance our mechanistic understanding of JMJ proteins' contributions to plant abiotic stress resilience, particularly in the context of salt stress adaptation.

JmjC结构域蛋白家族（JMJs）代表了一个对表观遗传调控和协调植物生长发育过程至关重要的关键去甲基化酶家族。尽管JMJ蛋白具有既定的作用，但对其在非生物胁迫条件下在玉米中的功能研究仍然有限。在这项研究中，我们鉴定并功能表征了ZmJMJ703，这是一个含JmjC结构域的基因，在玉米中表现出盐胁迫响应的表达模式。生理和表型分析表明，ZmJMJ703突变显著降低了玉米幼苗的耐盐性。转录组学分析揭示了zmjmj703突变体与野生型植物之间的差异表达模式，受影响的基因主要与细胞内蛋白质运输、氨基酸代谢和小分子重编程相关。通过质谱分析的平行蛋白质组学分析进一步表明，突变体中蛋白质的差异积累主要富集了与次生代谢物生物合成相关的途径。这些综合组学分析共同表明，ZmJMJ703可能调节对非生物应激反应至关重要的代谢途径。通过对异源过表达ZmJMJ703的拟南芥品系进行表型评价，证实其耐盐性强于对照植株。总的来说，这些发现显著地促进了我们对JMJ蛋白对植物非生物胁迫恢复能力的贡献的机制理解，特别是在盐胁迫适应的背景下。

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引用次数: 0

Golden coloration of Ginkgo biloba can be driven by fine-tuning of pigment, flavonoid, and terpene metabolism 银杏的金色是由色素、类黄酮和萜烯代谢的微调驱动的。

IF 4.1 3区生物学 Q1 PLANT SCIENCES

Journal of plant physiology

Pub Date : 2025-12-13 DOI: 10.1016/j.jplph.2025.154675

Yibin Lu , Guolin Wang , Dong Yang , Cuiping Zhang , Guo He , Xiao Zhou , Yu Liu , Weiqi Li , Chunxiang Fu , Mengzhu Lu , Gongke Zhou , Jie Meng

The ginkgo leaf, with its unique fan-shaped structure, golden color, and rich content of bioactive metabolites, serves as an important medium for both cultural appreciation and medicinal use. However, the high-resolution metabolic profile of pigments and bioactive compounds has yet to be systematically investigated during the leaf color change process. In this study, we investigated a yellow-leaf mutant (YL^m) and a naturally yellowing leaf type (YLⁿ), comparing them with green leaves (GL) in terms of cellular structure, metabolic profile of gene expression and metabolite contents, and hormone levels. First, only the light-harvesting complexes (LHCs) involved in the photosystems were severely damaged in YL^m while the whole chloroplast severely damaged in YLⁿ. Second, extensive reduction in chlorophyll content was only caused by the differential expression of POR, CAO and CLH in YL^m without the degradation which also occurred in the YLⁿ. The overall gene expression patterns as well as the proportion of specific metabolites in the carotenoid and flavonoid metabolic pathways varied significantly between YL^m and YLⁿ, suggesting distinct regulatory mechanisms between the two types of YL. The contents of hormones such as indole-3-acetic acid, jasmonic acid, ethylene levels, and gibberellin were significantly different between YL^m and YLⁿ. The expression levels of several transcription factors involved in chloroplast development and pigment biosynthesis such as GLK, FtsZ, ELIP, ORANGE, TCP14 were not changed significantly in YL^m. In conclusion, golden coloration of Ginkgo biloba is directly caused by the sharp decrease in chlorophyll, which can be driven by the precise regulation of certain genes and does not necessitate the initiation of senescence.

银杏叶以其独特的扇形结构、金黄色和丰富的生物活性代谢物，是一种重要的文化观赏和药用介质。然而，在叶片颜色变化过程中，色素和生物活性化合物的高分辨率代谢谱尚未得到系统的研究。在这项研究中，我们研究了一个黄叶突变体（YLm）和一个自然黄叶型（YLn），并在细胞结构、基因表达的代谢谱、代谢物含量和激素水平方面与绿叶（GL）进行了比较。首先，在YLn中，只有参与光系统的光收集复合物（lhc）受到严重破坏，而在YLn中，整个叶绿体都受到严重破坏。其次，叶绿素含量的大量降低仅仅是由YLm中POR、CAO和CLH的差异表达引起的，而YLn中也发生了降解。在类胡萝卜素和类黄酮代谢途径中，两种YL的整体基因表达模式和特定代谢物比例差异显著，表明两种YL的调控机制不同，吲哚-3-乙酸、茉莉酸、乙烯、赤霉素等激素含量在两种YL之间差异显著。GLK、FtsZ、ELIP、ORANGE、TCP14等参与叶绿体发育和色素生物合成的转录因子在YLm中的表达水平无显著变化。综上所述，银杏的金黄色是由叶绿素急剧下降直接引起的，可以通过某些基因的精确调控来驱动，而不一定是衰老的开始。

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引用次数: 0

Eggplant SmMYB6.2 positively regulates anthocyanin biosynthesis by activating SmANS gene expression 茄子SmMYB6.2通过激活SmANS基因表达正向调节花青素生物合成

IF 4.1 3区生物学 Q1 PLANT SCIENCES

Journal of plant physiology

Pub Date : 2025-12-13 DOI: 10.1016/j.jplph.2025.154676

Jiangnan Hao, Ziyi Hua, Jinwei Zhang, Sufen Liu, Dalu Li, Shaohang Li, Yang Liu, Huoying Chen

Anthocyanins significantly influence both the visual quality and nutritional value of eggplants. Exogenous application of jasmonic acid enhanced anthocyanin biosynthesis in eggplant peel under low-light conditions and induced the expression of several MYB genes. In this paper, these MYB proteins were investigated by yeast one-hybrid experiments, and it was found that SmMYB6.2 could directly bind to the promoter sequence of the anthocyanin synthesis structural gene SmANS. SmMYB6.2 was a nuclear-localized protein whose expression could be induced by various stimuli, including UV-B radiation, blue light, ABA treatment, PEG stress, and low-temperature exposure at 4 °C. Next, overexpression of SmMYB6.2 in Arabidopsis promoted anthocyanin accumulation and enhanced the gene expression of AtANS. Further, Dual-LUC assays demonstrated that SmMYB6.2 enhanced its transcriptional activation of the SmANS promoter through protein-protein interactions with the bHLH proteins SmTT8, SmbHLH79, and SmGLABRA3. These findings deepen our understanding of the regulatory mechanisms underlying anthocyanin biosynthesis in eggplant peel and provide candidate genes for breeding anthocyanin-enriched eggplant varieties.

花青素对茄子的视觉品质和营养价值均有显著影响。外源施用茉莉酸促进了弱光条件下茄子皮花青素的合成，诱导了多个MYB基因的表达。本文通过酵母单杂交实验对这些MYB蛋白进行了研究，发现SmMYB6.2可以直接结合花青素合成结构基因SmANS的启动子序列。SmMYB6.2是一种核定位蛋白，可以在各种刺激下诱导表达，包括UV-B辐射、蓝光、ABA处理、PEG胁迫和4°C低温暴露。接下来，SmMYB6.2在拟南芥中的过表达促进了花青素的积累，增强了AtANS基因的表达。此外，Dual-LUC分析表明，SmMYB6.2通过与bHLH蛋白SmTT8、SmbHLH79和SmGLABRA3的蛋白相互作用增强了其对SmANS启动子的转录激活。这些发现加深了我们对茄子皮花青素生物合成调控机制的理解，并为培育富含花青素的茄子品种提供了候选基因。

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