U. Hofmann, C. Moll, G. Engler, A. Engel, M. Bar, D. Hohl, U. Thomas, P. Detemple
This study describes a novel multisite recording probe with linear arrangement of recording sites. It is build from a 30 cm long, 650mum diameter stainless steel rod featuring 31 microelectrodes on the side of its business end and a conical tip. Recording sites are gold plated and have a complex impedance in saline of few hundred kOhm. It is manufactured by precision mechanics and connected with a low-noise multichannel amplifier to a medical grade recording setup. While mounted to a standard stereotaxic frame its movement in z-direction is controlled with a proven rubber motor. Preliminary recordings from anesthetized ferret's cortex show its ability to record both local field potentials and multi unit activity of neurons across different layers
{"title":"Multisite Microelectrodes for Use in Human Deep Brain Stimulation","authors":"U. Hofmann, C. Moll, G. Engler, A. Engel, M. Bar, D. Hohl, U. Thomas, P. Detemple","doi":"10.1109/MMB.2006.251551","DOIUrl":"https://doi.org/10.1109/MMB.2006.251551","url":null,"abstract":"This study describes a novel multisite recording probe with linear arrangement of recording sites. It is build from a 30 cm long, 650mum diameter stainless steel rod featuring 31 microelectrodes on the side of its business end and a conical tip. Recording sites are gold plated and have a complex impedance in saline of few hundred kOhm. It is manufactured by precision mechanics and connected with a low-noise multichannel amplifier to a medical grade recording setup. While mounted to a standard stereotaxic frame its movement in z-direction is controlled with a proven rubber motor. Preliminary recordings from anesthetized ferret's cortex show its ability to record both local field potentials and multi unit activity of neurons across different layers","PeriodicalId":170356,"journal":{"name":"2006 International Conference on Microtechnologies in Medicine and Biology","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130304486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
We demonstrated the effectiveness of introduction of charged nanoparticle-DNA conjugate for detection sensitivity of the genetic field effect transistor (FET), of which the principle is based on the potentiometric detection of charge density change on the gate surface. The large amount of negative charges on the gate insulator induced strongly the electrostatic interaction with electrons in silicon crystal by field effect resulting in the bigger shift of threshold voltage. The genetic FET platform combined with the charged nanoparticle-DNA conjugate is suitable for a simple, sensitive, accurate and inexpensive system for DNA analyses in clinical diagnostics
{"title":"Detection sensitivity of genetic field effect transistor combined with charged nanoparticle-DNA conjugate","authors":"T. Sakata, Y. Miyahara","doi":"10.1109/MMB.2006.251500","DOIUrl":"https://doi.org/10.1109/MMB.2006.251500","url":null,"abstract":"We demonstrated the effectiveness of introduction of charged nanoparticle-DNA conjugate for detection sensitivity of the genetic field effect transistor (FET), of which the principle is based on the potentiometric detection of charge density change on the gate surface. The large amount of negative charges on the gate insulator induced strongly the electrostatic interaction with electrons in silicon crystal by field effect resulting in the bigger shift of threshold voltage. The genetic FET platform combined with the charged nanoparticle-DNA conjugate is suitable for a simple, sensitive, accurate and inexpensive system for DNA analyses in clinical diagnostics","PeriodicalId":170356,"journal":{"name":"2006 International Conference on Microtechnologies in Medicine and Biology","volume":"69 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132419578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
T. Akalin, S. Arscott, B. Bocquet, N. Bourzgui, V. Mille, V. Senez, A. Treizebré
By performing dielectric spectroscopy across a wide spectrum of frequencies (DC-THz), single-cell analysis could be performed without labeling process. That is why we are developing different micro structures, either planar/3D electrodes or transmission lines, and microfluidic devices using BIOMEMS technologies. In our devices, silicon or glass substrates are combined with polymeric materials and gold patterns. For low frequency (LF) applications, PDMS has been chosen while for high frequency (HF) structures, the PPTMDSO is preferred. We are designing the devices thanks to modeling and simulation using either COMSOL (LF) or CST (HF) softwares. Different designs have already been fabricated and are presented in this paper. Finally, we have performed single cell measurements for the low frequency range (<10 MHz) and the next step will focuse on high frequency measurements
{"title":"Microfluidic Devices for Ultra Wide Band Electromagnetic Spectroscopy of Single Cell","authors":"T. Akalin, S. Arscott, B. Bocquet, N. Bourzgui, V. Mille, V. Senez, A. Treizebré","doi":"10.1109/MMB.2006.251542","DOIUrl":"https://doi.org/10.1109/MMB.2006.251542","url":null,"abstract":"By performing dielectric spectroscopy across a wide spectrum of frequencies (DC-THz), single-cell analysis could be performed without labeling process. That is why we are developing different micro structures, either planar/3D electrodes or transmission lines, and microfluidic devices using BIOMEMS technologies. In our devices, silicon or glass substrates are combined with polymeric materials and gold patterns. For low frequency (LF) applications, PDMS has been chosen while for high frequency (HF) structures, the PPTMDSO is preferred. We are designing the devices thanks to modeling and simulation using either COMSOL (LF) or CST (HF) softwares. Different designs have already been fabricated and are presented in this paper. Finally, we have performed single cell measurements for the low frequency range (<10 MHz) and the next step will focuse on high frequency measurements","PeriodicalId":170356,"journal":{"name":"2006 International Conference on Microtechnologies in Medicine and Biology","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123735372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
C. Provin, K. Takano, R. Shirakashi, Y. Sakai, T. Fujii
In order to perform cell culture from soft tissue in good condition, i.e. maintaining high viability and keeping specific physiological function, scaffolds with microstructured surface mimicking in vivo environment is necessary. In this paper we proposed PDMS microbioreactors with high surface/volume ratio for hepatocyte culture. These PDMS microbioreactors were designed for cells to attach in high density (over 108 cells/cm3), and were characterized in terms of flow resistance, which is important for mass transport of nutrients and oxygen
{"title":"Fabrication of Microbioreactors with an Optimized Structure Designed for High Density Culture of Hepatocyte","authors":"C. Provin, K. Takano, R. Shirakashi, Y. Sakai, T. Fujii","doi":"10.1109/MMB.2006.251546","DOIUrl":"https://doi.org/10.1109/MMB.2006.251546","url":null,"abstract":"In order to perform cell culture from soft tissue in good condition, i.e. maintaining high viability and keeping specific physiological function, scaffolds with microstructured surface mimicking in vivo environment is necessary. In this paper we proposed PDMS microbioreactors with high surface/volume ratio for hepatocyte culture. These PDMS microbioreactors were designed for cells to attach in high density (over 108 cells/cm3), and were characterized in terms of flow resistance, which is important for mass transport of nutrients and oxygen","PeriodicalId":170356,"journal":{"name":"2006 International Conference on Microtechnologies in Medicine and Biology","volume":"102 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125704429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Popa, E. Vasilescu, P. Drob, D. Iordăchescu, A. Cîmpean, D. Ionita, C. Vasilescu
Studies concerning the long-term electrochemical behavior in artificial saliva and interactions with fibroblasts of Ti-45.5Ni bioalloy are performed in this paper. Titanium and its Ti-45.5Ni alloy revealed a typical passive metal behavior in Tani&Zucchi (of pH=2.5 and 7) and Fusayama (pH=5.5) artificial saliva. Breakdown (pitting) potential has electropositive values that can not be reached in oral cavity. Corrosion potentials for Ti-45.5Ni alloy in Carter-Brugirard saliva presented more electronegative values than titanium, but no form of local corrosion was registered during 4000 exposure hours. Corrosion potential gradients simulated for saliva composition non-uniformity have low values, showing very good corrosion resistance. Human embryonic fibroblasts rapidly adhered (72% cells in 48 hours) on Ti-45.5Ni alloy surface. Cell proliferation is considerable in the first 48 hours of culture. Slight cytotoxic effects specially developed after 72 incubation hours
{"title":"In vitro interactions of titanium bioalloys with human cells and physiological fluids","authors":"M. Popa, E. Vasilescu, P. Drob, D. Iordăchescu, A. Cîmpean, D. Ionita, C. Vasilescu","doi":"10.1109/MMB.2006.251496","DOIUrl":"https://doi.org/10.1109/MMB.2006.251496","url":null,"abstract":"Studies concerning the long-term electrochemical behavior in artificial saliva and interactions with fibroblasts of Ti-45.5Ni bioalloy are performed in this paper. Titanium and its Ti-45.5Ni alloy revealed a typical passive metal behavior in Tani&Zucchi (of pH=2.5 and 7) and Fusayama (pH=5.5) artificial saliva. Breakdown (pitting) potential has electropositive values that can not be reached in oral cavity. Corrosion potentials for Ti-45.5Ni alloy in Carter-Brugirard saliva presented more electronegative values than titanium, but no form of local corrosion was registered during 4000 exposure hours. Corrosion potential gradients simulated for saliva composition non-uniformity have low values, showing very good corrosion resistance. Human embryonic fibroblasts rapidly adhered (72% cells in 48 hours) on Ti-45.5Ni alloy surface. Cell proliferation is considerable in the first 48 hours of culture. Slight cytotoxic effects specially developed after 72 incubation hours","PeriodicalId":170356,"journal":{"name":"2006 International Conference on Microtechnologies in Medicine and Biology","volume":"271 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116835439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.1109/crmico.2006.256359
The document that should appear here is not currently available.
这里应该出现的文档目前不可用。
{"title":"PDF Not Yet Available In IEEE Xplore","authors":"","doi":"10.1109/crmico.2006.256359","DOIUrl":"https://doi.org/10.1109/crmico.2006.256359","url":null,"abstract":"The document that should appear here is not currently available.","PeriodicalId":170356,"journal":{"name":"2006 International Conference on Microtechnologies in Medicine and Biology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129755770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.1109/td-asia.2009.5357022
The document that should appear here is not currently available.
这里应该出现的文档目前不可用。
{"title":"PDF Not Yet Available In IEEE Xplore","authors":"","doi":"10.1109/td-asia.2009.5357022","DOIUrl":"https://doi.org/10.1109/td-asia.2009.5357022","url":null,"abstract":"The document that should appear here is not currently available.","PeriodicalId":170356,"journal":{"name":"2006 International Conference on Microtechnologies in Medicine and Biology","volume":"47 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123687901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.1109/nebc.2011.5778715
The document that should appear here is not currently available.
这里应该出现的文档目前不可用。
{"title":"PDF Not Yet Available In IEEE Xplore","authors":"","doi":"10.1109/nebc.2011.5778715","DOIUrl":"https://doi.org/10.1109/nebc.2011.5778715","url":null,"abstract":"The document that should appear here is not currently available.","PeriodicalId":170356,"journal":{"name":"2006 International Conference on Microtechnologies in Medicine and Biology","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116051645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.1109/telsks.2007.4375943
The document that should appear here is not currently available.
这里应该出现的文档目前不可用。
{"title":"PDF Not Yet Available In IEEE Xplore","authors":"","doi":"10.1109/telsks.2007.4375943","DOIUrl":"https://doi.org/10.1109/telsks.2007.4375943","url":null,"abstract":"The document that should appear here is not currently available.","PeriodicalId":170356,"journal":{"name":"2006 International Conference on Microtechnologies in Medicine and Biology","volume":"51 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128871356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：