We have been developing a measurement system for membrane transport across a reconstituted planar lipid bilayer to examine the functions of transporter membrane proteins using a microchamber array (membrane microchamber system). In this system, an artificial planar lipid bilayer is pressed on the parylene microchamber array (typically 0.1~1 pL in volume) fabricated on a coverglass to enclose the volume. After membrane proteins are reconstituted in the bilayer, fluorescently labeled molecules transported across the bilayer are accumulated in microchambers, and detected by fluorescent imaging. The microchamber array is compatible with high-sensitive biological imaging techniques, such as a confocal microscopy and a total internal reflection fluorescence microscopy (TIRFM). Due to the tiny volume of microchambers, concentration of the transported molecules rapidly increases. In this study, the detection system was characterized in detail. With the aid of microchambers, the concentration reached to the detectable level with as small as 102~103 fluorescent molecules
{"title":"Characterization of the Membrane Transport Assay System Using Microchamber Array","authors":"H. Suzuki, K. Tabata, H. Noji, S. Takeuchi","doi":"10.1109/MMB.2006.251556","DOIUrl":"https://doi.org/10.1109/MMB.2006.251556","url":null,"abstract":"We have been developing a measurement system for membrane transport across a reconstituted planar lipid bilayer to examine the functions of transporter membrane proteins using a microchamber array (membrane microchamber system). In this system, an artificial planar lipid bilayer is pressed on the parylene microchamber array (typically 0.1~1 pL in volume) fabricated on a coverglass to enclose the volume. After membrane proteins are reconstituted in the bilayer, fluorescently labeled molecules transported across the bilayer are accumulated in microchambers, and detected by fluorescent imaging. The microchamber array is compatible with high-sensitive biological imaging techniques, such as a confocal microscopy and a total internal reflection fluorescence microscopy (TIRFM). Due to the tiny volume of microchambers, concentration of the transported molecules rapidly increases. In this study, the detection system was characterized in detail. With the aid of microchambers, the concentration reached to the detectable level with as small as 102~103 fluorescent molecules","PeriodicalId":170356,"journal":{"name":"2006 International Conference on Microtechnologies in Medicine and Biology","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131552717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The intraosseous pressure generation and fluid flow in lacunocanalicular network of trabeculae could be important to the remodeling process of cancellous bone. Due to the technical difficulty, experimental information about the amount of intraosseous pressure generation in lacunocanalicular network of trabeculae is never measured. In this study, the maximum intraosseous pressure generation in micro-trabecular specimens within the elastic range was measured in vitro using a specially designed micro-experimental setup representing the most restrictive fluid flow boundaries around microscopic bovine vertebral trabecular specimens. Then, a quasi-static loading (9mum/min) was applied up to the strain of 0.4% with measuring intraosseous pressure generations in the undrained and drained conditions. 49.2plusmn4.45 KPa of intraosseous pressure generation at the 0.4% strain was found in the undrained condition. In contrast, no intraosseous pressure generation was measured in the drained condition. The result could let us know the amount of a possible maximum intraosseous pressure generation in lacunocanalicular network of trabeculae within the elastic range
{"title":"Fluid Pressure Measurement in Lacunocanalicular Network of Trabeculae Using MEMS Based Micro-Pressure Transducer","authors":"Junghwa Hong, Young Hwan Park, H. Cha","doi":"10.1109/MMB.2006.251538","DOIUrl":"https://doi.org/10.1109/MMB.2006.251538","url":null,"abstract":"The intraosseous pressure generation and fluid flow in lacunocanalicular network of trabeculae could be important to the remodeling process of cancellous bone. Due to the technical difficulty, experimental information about the amount of intraosseous pressure generation in lacunocanalicular network of trabeculae is never measured. In this study, the maximum intraosseous pressure generation in micro-trabecular specimens within the elastic range was measured in vitro using a specially designed micro-experimental setup representing the most restrictive fluid flow boundaries around microscopic bovine vertebral trabecular specimens. Then, a quasi-static loading (9mum/min) was applied up to the strain of 0.4% with measuring intraosseous pressure generations in the undrained and drained conditions. 49.2plusmn4.45 KPa of intraosseous pressure generation at the 0.4% strain was found in the undrained condition. In contrast, no intraosseous pressure generation was measured in the drained condition. The result could let us know the amount of a possible maximum intraosseous pressure generation in lacunocanalicular network of trabeculae within the elastic range","PeriodicalId":170356,"journal":{"name":"2006 International Conference on Microtechnologies in Medicine and Biology","volume":"197 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132187611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. Goda, Y. Yamamoto, T. Nakamura, T. Kusuhara, S. Mohri, N. Kataoka, F. Kajiya
We constructed a precision mathematical model for quantitative evaluation of the micro-dynamics of cultured cells measured with ECIS (electrical cell-substrate impedance sensing) system that could separately estimate cell-to-cell distance and cell-to-substrate distance. For wide applications of this method, we constructed mathematical models describing for some kinds of cell and some kinds of confluent conditions. The mathematical models of these impedances could be fit well especially from 1 kHz to 10 kHz, which were most interesting frequency range for micro-dynamic analysis in ECIS methods
{"title":"Quantitative Evaluation of Micro-motion of Vascular Endothelial Cells in Electrical Cell-substrate Impedance Sensing (ECIS) Method Using a Precision Mathematical Model","authors":"N. Goda, Y. Yamamoto, T. Nakamura, T. Kusuhara, S. Mohri, N. Kataoka, F. Kajiya","doi":"10.1109/MMB.2006.251480","DOIUrl":"https://doi.org/10.1109/MMB.2006.251480","url":null,"abstract":"We constructed a precision mathematical model for quantitative evaluation of the micro-dynamics of cultured cells measured with ECIS (electrical cell-substrate impedance sensing) system that could separately estimate cell-to-cell distance and cell-to-substrate distance. For wide applications of this method, we constructed mathematical models describing for some kinds of cell and some kinds of confluent conditions. The mathematical models of these impedances could be fit well especially from 1 kHz to 10 kHz, which were most interesting frequency range for micro-dynamic analysis in ECIS methods","PeriodicalId":170356,"journal":{"name":"2006 International Conference on Microtechnologies in Medicine and Biology","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122001673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
We present a micro-fluidic technique for the evaluation of the blood compatibility of nano-structured surfaces under flow conditions. Micro-fluidic chips are coated with a polymer film. The demixing behavior of an immiscible polymer blend is used to create structured surfaces having typical feature sizes ranging from the nanometer to the micrometer length scale. Whole human blood is used to evaluate the blood interaction with these surfaces in terms of platelet adhesion, while platelet poor plasma is used to investigate protein adsorption. Our studies indicate that increasing the feature size of the surface nano-structures encourages von Willebrand factor adsorption and thus platelet adhesion and consequent thrombus formation
{"title":"A Micro-Fluidic Technique for the Evaluation of the Blood Compatibility of Nanostructured Polymer Surfaces","authors":"C. Minelli, A. Kikuta, A. Yamamoto","doi":"10.1109/MMB.2006.251539","DOIUrl":"https://doi.org/10.1109/MMB.2006.251539","url":null,"abstract":"We present a micro-fluidic technique for the evaluation of the blood compatibility of nano-structured surfaces under flow conditions. Micro-fluidic chips are coated with a polymer film. The demixing behavior of an immiscible polymer blend is used to create structured surfaces having typical feature sizes ranging from the nanometer to the micrometer length scale. Whole human blood is used to evaluate the blood interaction with these surfaces in terms of platelet adhesion, while platelet poor plasma is used to investigate protein adsorption. Our studies indicate that increasing the feature size of the surface nano-structures encourages von Willebrand factor adsorption and thus platelet adhesion and consequent thrombus formation","PeriodicalId":170356,"journal":{"name":"2006 International Conference on Microtechnologies in Medicine and Biology","volume":"32 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127282234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J. Stéen, R. Kerkhofs, V. Blech, J. Brugger, B. Kim
We have developed a hybrid scanning probe microscopy sensor consisting of a SU-8 body and full platinum cantilever and tip. The fabrication process is based on surface-micromachining of a silicon wafer, where oxidation sharpened moulds define the tip shape, giving tips with a radius sharper than 20 nm. With optimized sputter deposition parameters we obtained fully straight platinum cantilevers after release. The release is based on a dry isotropic silicon under-etching. Platinum shows biocompatibility, making the sensors suitable for imaging and force spectroscopy of living cells. A resonance frequency of 252 KHz and a mechanical quality factor of 56 are measured in air for a cantilever of 35 mumtimes20 mumtimes0.5 mum. A method for the immobilization of cells in cell-containers is developed to simplify their localization and imaging
{"title":"Novel full platinum nanoprobes suitable for biological SPM experiments","authors":"J. Stéen, R. Kerkhofs, V. Blech, J. Brugger, B. Kim","doi":"10.1109/MMB.2006.251552","DOIUrl":"https://doi.org/10.1109/MMB.2006.251552","url":null,"abstract":"We have developed a hybrid scanning probe microscopy sensor consisting of a SU-8 body and full platinum cantilever and tip. The fabrication process is based on surface-micromachining of a silicon wafer, where oxidation sharpened moulds define the tip shape, giving tips with a radius sharper than 20 nm. With optimized sputter deposition parameters we obtained fully straight platinum cantilevers after release. The release is based on a dry isotropic silicon under-etching. Platinum shows biocompatibility, making the sensors suitable for imaging and force spectroscopy of living cells. A resonance frequency of 252 KHz and a mechanical quality factor of 56 are measured in air for a cantilever of 35 mumtimes20 mumtimes0.5 mum. A method for the immobilization of cells in cell-containers is developed to simplify their localization and imaging","PeriodicalId":170356,"journal":{"name":"2006 International Conference on Microtechnologies in Medicine and Biology","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132110070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Y. Kato, M. Nishino, I. Saito, T. Suzuki, K. Mabuchi
A flexible intracortical neural probe containing a biodegradable polymer for delivering bioactive components was developed. This was designed to promote regrowth of damaged neural tissues around the implanted neural probe for a long-term recording. The neural probe was based on the flexible and biocompatible material of parylene C incorporated a drug delivery system (DDS). A groove structure of the probe was designed to seed the degradable polymeric microspheres with bioactive components, to promote recovery of the damaged tissues, and improve mechanical stiffness for the probe implantation. The efficacy of released nerve growth factor (NGF) from the microspheres was observed in in vitro experiments with PC12 cells. The neural probe was successfully inserted in the cerebral cortex of a rat, and neural signals were recorded. These results have shown the possibility that the flexible intracortical neural probe can be applied for chronic recording along with neural regeneration
{"title":"Flexible Intracortical Neural Probe with Biodegradable Polymer for Delivering Bioactive Components","authors":"Y. Kato, M. Nishino, I. Saito, T. Suzuki, K. Mabuchi","doi":"10.1109/MMB.2006.251512","DOIUrl":"https://doi.org/10.1109/MMB.2006.251512","url":null,"abstract":"A flexible intracortical neural probe containing a biodegradable polymer for delivering bioactive components was developed. This was designed to promote regrowth of damaged neural tissues around the implanted neural probe for a long-term recording. The neural probe was based on the flexible and biocompatible material of parylene C incorporated a drug delivery system (DDS). A groove structure of the probe was designed to seed the degradable polymeric microspheres with bioactive components, to promote recovery of the damaged tissues, and improve mechanical stiffness for the probe implantation. The efficacy of released nerve growth factor (NGF) from the microspheres was observed in in vitro experiments with PC12 cells. The neural probe was successfully inserted in the cerebral cortex of a rat, and neural signals were recorded. These results have shown the possibility that the flexible intracortical neural probe can be applied for chronic recording along with neural regeneration","PeriodicalId":170356,"journal":{"name":"2006 International Conference on Microtechnologies in Medicine and Biology","volume":"106 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131411476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Popa, D. Iordăchescu, I. Demetrescu, E. Vasilescu, P. Drob, A. Cîmpean, M. Istratescu, C. Vasilescu
In vitro osteoblasts/Ti-6Al-7Nb bioalloy interactions were characterized in this paper. Also, the electrochemical properties of the passive films on titanium and its Ti-6Al-7Nb bioalloy in Ringer 2 and 3 solutions were determined as an aspect of their biocompatibility. Human osteoblast proliferation and viability are very good. "Cell effort" to adhere on metallic support is visualized by some morphologic changes of the osteoblasts. Cytotoxicity effects of Ti-6Al-7Nb alloy appeared after 48 culture hours and decreased in time, demonstrating a very good biocompatibility. In Ringer 2 solution of different pH values (2.5; 5; 6.7 and 9), titanium and Ti-6Al-7Nb alloy presented self-passivation, very large passive potential range, very low passive current densities, confirming a compact, protective, stable layer. The very low corrosion rates and ion release values prove a near imperceptible toxicity. In Ringer 3 solution, open circuit potentials of titanium and its Ti-6Al-7Nb alloy tend to electropositive values, attesting a process of passivity increase by thickening
{"title":"In vitro biocompatibility and electrochemical behavior of titanium and its alloys","authors":"M. Popa, D. Iordăchescu, I. Demetrescu, E. Vasilescu, P. Drob, A. Cîmpean, M. Istratescu, C. Vasilescu","doi":"10.1109/MMB.2006.251478","DOIUrl":"https://doi.org/10.1109/MMB.2006.251478","url":null,"abstract":"In vitro osteoblasts/Ti-6Al-7Nb bioalloy interactions were characterized in this paper. Also, the electrochemical properties of the passive films on titanium and its Ti-6Al-7Nb bioalloy in Ringer 2 and 3 solutions were determined as an aspect of their biocompatibility. Human osteoblast proliferation and viability are very good. \"Cell effort\" to adhere on metallic support is visualized by some morphologic changes of the osteoblasts. Cytotoxicity effects of Ti-6Al-7Nb alloy appeared after 48 culture hours and decreased in time, demonstrating a very good biocompatibility. In Ringer 2 solution of different pH values (2.5; 5; 6.7 and 9), titanium and Ti-6Al-7Nb alloy presented self-passivation, very large passive potential range, very low passive current densities, confirming a compact, protective, stable layer. The very low corrosion rates and ion release values prove a near imperceptible toxicity. In Ringer 3 solution, open circuit potentials of titanium and its Ti-6Al-7Nb alloy tend to electropositive values, attesting a process of passivity increase by thickening","PeriodicalId":170356,"journal":{"name":"2006 International Conference on Microtechnologies in Medicine and Biology","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131481435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
C. Yamahata, T. Takekawa, K. Ayano, M. Hosogi, M. Kumemura, B. Legrand, D. Collard, G. Hashiguchi, H. Fujita
We describe electrostatically actuated silicon nanotweezers which are intended for the manipulation and characterization of DNA molecules. The fabrication process combines KOH etching and deep reactive ion etching (DRIE) on silicon-on-insulator (SOI) wafer to form sharp nanotips and high aspect ratio microstructures, respectively. The microelectromechanical system (MEMS) consists of a pair of opposing tips, the distance of which can be accurately adjusted thanks to a high resolution differential capacitive sensor. The device shows a resolution of 5 nm for a displacement range of 3 mum (static mode). It has a resonant frequency at 2 kHz and a quality factor of 40 in air, and 550 in vacuum
{"title":"Silicon Nanotweezers with Adjustable and Controllable Gap for the Manipulation and Characterization of DNA Molecules","authors":"C. Yamahata, T. Takekawa, K. Ayano, M. Hosogi, M. Kumemura, B. Legrand, D. Collard, G. Hashiguchi, H. Fujita","doi":"10.1109/MMB.2006.251507","DOIUrl":"https://doi.org/10.1109/MMB.2006.251507","url":null,"abstract":"We describe electrostatically actuated silicon nanotweezers which are intended for the manipulation and characterization of DNA molecules. The fabrication process combines KOH etching and deep reactive ion etching (DRIE) on silicon-on-insulator (SOI) wafer to form sharp nanotips and high aspect ratio microstructures, respectively. The microelectromechanical system (MEMS) consists of a pair of opposing tips, the distance of which can be accurately adjusted thanks to a high resolution differential capacitive sensor. The device shows a resolution of 5 nm for a displacement range of 3 mum (static mode). It has a resonant frequency at 2 kHz and a quality factor of 40 in air, and 550 in vacuum","PeriodicalId":170356,"journal":{"name":"2006 International Conference on Microtechnologies in Medicine and Biology","volume":"71 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134492234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
We present a biologically inspired sensing method which can be used to detect toxins or other chemical compounds. We engineer our hydrogels in such a way that an elastic instability is triggered by a designated stimulus. Two different bi-polymer strips are fixed together. When swelling is induced, the differential response of the two gels forming the composite strip causes the strip to bend. If the bending is constrained, an explosive elastic instability can occur if either the constraint is removed or the stored elastic energy is enough to induce fracture. A judicious choice of materials (e.g., degradable adhesive materials specific to certain enzymes or chemicals) and geometric constraints allow for the tuning of the instability. The rapid geometric changes associated with the elastic instability can be easily observed
{"title":"A Sensing Method based on Elastic Instabilities of Swelllng Hydrogels","authors":"D. Kim, D. Beebe","doi":"10.1109/MMB.2006.251553","DOIUrl":"https://doi.org/10.1109/MMB.2006.251553","url":null,"abstract":"We present a biologically inspired sensing method which can be used to detect toxins or other chemical compounds. We engineer our hydrogels in such a way that an elastic instability is triggered by a designated stimulus. Two different bi-polymer strips are fixed together. When swelling is induced, the differential response of the two gels forming the composite strip causes the strip to bend. If the bending is constrained, an explosive elastic instability can occur if either the constraint is removed or the stored elastic energy is enough to induce fracture. A judicious choice of materials (e.g., degradable adhesive materials specific to certain enzymes or chemicals) and geometric constraints allow for the tuning of the instability. The rapid geometric changes associated with the elastic instability can be easily observed","PeriodicalId":170356,"journal":{"name":"2006 International Conference on Microtechnologies in Medicine and Biology","volume":"33 7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131031470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A simple and effective method for patterning cell on glass substrate is reported. A passivation layer that is capable of preventing cell adhesion was first coated onto glass surface. The passivation coatings that were tested include 3-[Bis(2-hydroxyethyl)amino]propyl-triethoxysilane, 3-Aminopropyltriethoxysilane, 1H, 1H, 2H, 2H-Perfluorodecyl-triethoxysilane, 3 -Glycidyloxypropyltriethoxysilane, Octade cyltrimethoxysilane, and Octadecyltrichlorosilane. A CO2 laser and a UV laser were used to remove the passivation layer and define patterns according to user design. Cell arrays and arbitrary pattern were demonstrated. The minimal feature attainable by the CO2 laser is about 100 mum. The minimal feature for reliable cell adhesion is found to be about 25 mum. Cells adhere and grow cleanly in the laser defined pattern. The pattern can be re-designed and fabricated easily and rapidly. Living cell staining by CMFDA and antibody staining for epidermal growth factor receptor (EGFR) detection were successfully performed on the surface bound cells. This method can be very useful for developing drug screening tools that utilize cell-based detection
{"title":"Maskless direct cell patterning by laser writing","authors":"J.Y. Cheng, Hsueh-Yi Lee, M. Yen, T. Young","doi":"10.1109/MMB.2006.251550","DOIUrl":"https://doi.org/10.1109/MMB.2006.251550","url":null,"abstract":"A simple and effective method for patterning cell on glass substrate is reported. A passivation layer that is capable of preventing cell adhesion was first coated onto glass surface. The passivation coatings that were tested include 3-[Bis(2-hydroxyethyl)amino]propyl-triethoxysilane, 3-Aminopropyltriethoxysilane, 1H, 1H, 2H, 2H-Perfluorodecyl-triethoxysilane, 3 -Glycidyloxypropyltriethoxysilane, Octade cyltrimethoxysilane, and Octadecyltrichlorosilane. A CO2 laser and a UV laser were used to remove the passivation layer and define patterns according to user design. Cell arrays and arbitrary pattern were demonstrated. The minimal feature attainable by the CO2 laser is about 100 mum. The minimal feature for reliable cell adhesion is found to be about 25 mum. Cells adhere and grow cleanly in the laser defined pattern. The pattern can be re-designed and fabricated easily and rapidly. Living cell staining by CMFDA and antibody staining for epidermal growth factor receptor (EGFR) detection were successfully performed on the surface bound cells. This method can be very useful for developing drug screening tools that utilize cell-based detection","PeriodicalId":170356,"journal":{"name":"2006 International Conference on Microtechnologies in Medicine and Biology","volume":"39 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116323891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：