The development of a neural interface that will allow signals from the human nervous system to control external equipment is extremely important for the next generation of prosthetic systems. A novel multichannel regeneration-type nerve electrode designed to record from and stimulate peripheral nerves has been developed to allow the control of artificial hands and to generate artificial sensations. In this study a novel flexible regeneration microelectrode based on the nerve regeneration principle was designed and fabricated using MEMS technologies. The electrode, which was fabricated on a 20-mum-thick parylene C substrate, has multiple fluidic channels. Each fluidic channel was 100 mum widetimes40 mum hightimes1500 mum long and featured multiple electrodes inside them as recording and stimulating sites. They also served as guidance channels for the regenerating axons. The authors are currently attempting to evaluate the probes using the sciatic nerve of rats
{"title":"Flexible Regeneration-type Nerve Electrode with Integrated Microfluidic Channels","authors":"T. Suzuki, N. Kotake, K. Mabuchi, S. Takeuchi","doi":"10.1109/MMB.2006.251557","DOIUrl":"https://doi.org/10.1109/MMB.2006.251557","url":null,"abstract":"The development of a neural interface that will allow signals from the human nervous system to control external equipment is extremely important for the next generation of prosthetic systems. A novel multichannel regeneration-type nerve electrode designed to record from and stimulate peripheral nerves has been developed to allow the control of artificial hands and to generate artificial sensations. In this study a novel flexible regeneration microelectrode based on the nerve regeneration principle was designed and fabricated using MEMS technologies. The electrode, which was fabricated on a 20-mum-thick parylene C substrate, has multiple fluidic channels. Each fluidic channel was 100 mum widetimes40 mum hightimes1500 mum long and featured multiple electrodes inside them as recording and stimulating sites. They also served as guidance channels for the regenerating axons. The authors are currently attempting to evaluate the probes using the sciatic nerve of rats","PeriodicalId":170356,"journal":{"name":"2006 International Conference on Microtechnologies in Medicine and Biology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122184117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A simple and effective method for patterning cell on glass substrate is reported. A passivation layer that is capable of preventing cell adhesion was first coated onto glass surface. The passivation coatings that were tested include 3-[Bis(2-hydroxyethyl)amino]propyl-triethoxysilane, 3-Aminopropyltriethoxysilane, 1H, 1H, 2H, 2H-Perfluorodecyl-triethoxysilane, 3 -Glycidyloxypropyltriethoxysilane, Octade cyltrimethoxysilane, and Octadecyltrichlorosilane. A CO2 laser and a UV laser were used to remove the passivation layer and define patterns according to user design. Cell arrays and arbitrary pattern were demonstrated. The minimal feature attainable by the CO2 laser is about 100 mum. The minimal feature for reliable cell adhesion is found to be about 25 mum. Cells adhere and grow cleanly in the laser defined pattern. The pattern can be re-designed and fabricated easily and rapidly. Living cell staining by CMFDA and antibody staining for epidermal growth factor receptor (EGFR) detection were successfully performed on the surface bound cells. This method can be very useful for developing drug screening tools that utilize cell-based detection
{"title":"Maskless direct cell patterning by laser writing","authors":"J.Y. Cheng, Hsueh-Yi Lee, M. Yen, T. Young","doi":"10.1109/MMB.2006.251550","DOIUrl":"https://doi.org/10.1109/MMB.2006.251550","url":null,"abstract":"A simple and effective method for patterning cell on glass substrate is reported. A passivation layer that is capable of preventing cell adhesion was first coated onto glass surface. The passivation coatings that were tested include 3-[Bis(2-hydroxyethyl)amino]propyl-triethoxysilane, 3-Aminopropyltriethoxysilane, 1H, 1H, 2H, 2H-Perfluorodecyl-triethoxysilane, 3 -Glycidyloxypropyltriethoxysilane, Octade cyltrimethoxysilane, and Octadecyltrichlorosilane. A CO2 laser and a UV laser were used to remove the passivation layer and define patterns according to user design. Cell arrays and arbitrary pattern were demonstrated. The minimal feature attainable by the CO2 laser is about 100 mum. The minimal feature for reliable cell adhesion is found to be about 25 mum. Cells adhere and grow cleanly in the laser defined pattern. The pattern can be re-designed and fabricated easily and rapidly. Living cell staining by CMFDA and antibody staining for epidermal growth factor receptor (EGFR) detection were successfully performed on the surface bound cells. This method can be very useful for developing drug screening tools that utilize cell-based detection","PeriodicalId":170356,"journal":{"name":"2006 International Conference on Microtechnologies in Medicine and Biology","volume":"39 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116323891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This paper describes a 15-channel wireless implantable neural recording (WINeR) system for long-term in vivo experiments. WINeRS consists of an implantable part that contains a system-on-a-chip (SoC) application-specific integrated circuit (ASIC) and an external receiver. The 3 mmtimes3 mm ASIC is fabricated in the MOSIS AMI 0.5-mum 3-poly 3-metal n-well standard CMOS process. The chip contains 15 low-noise amplifier/filters, time division multiplexer (TDM), sample-and-hold (S&H), pulse width modulator (PWM), on-chip clock generator, 32-bit register for control commands, ISM-band VCO transmitter, reference generator, and inductive power management circuitry. The use of PWM technique has lowered power consumption, improved robustness against noise, and reduced complexity by eliminating ADC and its associated circuitry. A commercial FM receiver is used as the external part of the system. The received PWM signal is further demodulated off-line by a MATLAB program. Finally by time division demultiplexing the demodulated samples, the original neural signals are being reconstructed. A custom wideband receiver with real-time PWM/TDM demodulator/demultiplexer is currently under construction
介绍了一种用于长期体内实验的15通道无线植入式神经记录(WINeR)系统。WINeRS由一个可植入的部分组成，其中包含一个系统单片(SoC)专用集成电路(ASIC)和一个外部接收器。3mmtimes3mm ASIC采用MOSIS AMI 0.5-mum 3-poly 3-metal n-well标准CMOS工艺制造。该芯片包含15个低噪声放大器/滤波器、时分多路复用器(TDM)、采样保持器(S&H)、脉宽调制器(PWM)、片上时钟发生器、32位控制命令寄存器、ism波段VCO发射机、参考发生器和感应电源管理电路。PWM技术的使用降低了功耗，提高了抗噪声的鲁棒性，并通过消除ADC及其相关电路降低了复杂性。一个商用调频接收机被用作系统的外部部分。接收到的PWM信号通过MATLAB程序进一步离线解调。最后对解调后的样本进行时分解复用，重建原始神经信号。一种带有实时PWM/TDM解调器/解复用器的定制宽带接收器目前正在建设中
{"title":"A 15-Channel Wireless Neural Recording System Based on Time Division Multiplexing of Pulse Width Modulated Signals","authors":"M. Yin, R. Field, Maysam Ghovanloo","doi":"10.1109/MMB.2006.251555","DOIUrl":"https://doi.org/10.1109/MMB.2006.251555","url":null,"abstract":"This paper describes a 15-channel wireless implantable neural recording (WINeR) system for long-term in vivo experiments. WINeRS consists of an implantable part that contains a system-on-a-chip (SoC) application-specific integrated circuit (ASIC) and an external receiver. The 3 mmtimes3 mm ASIC is fabricated in the MOSIS AMI 0.5-mum 3-poly 3-metal n-well standard CMOS process. The chip contains 15 low-noise amplifier/filters, time division multiplexer (TDM), sample-and-hold (S&H), pulse width modulator (PWM), on-chip clock generator, 32-bit register for control commands, ISM-band VCO transmitter, reference generator, and inductive power management circuitry. The use of PWM technique has lowered power consumption, improved robustness against noise, and reduced complexity by eliminating ADC and its associated circuitry. A commercial FM receiver is used as the external part of the system. The received PWM signal is further demodulated off-line by a MATLAB program. Finally by time division demultiplexing the demodulated samples, the original neural signals are being reconstructed. A custom wideband receiver with real-time PWM/TDM demodulator/demultiplexer is currently under construction","PeriodicalId":170356,"journal":{"name":"2006 International Conference on Microtechnologies in Medicine and Biology","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126451861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
T. Suzuki, Y. Teramura, K. Inokuma, I. Kanno, H. Iwata, H. Kotera
A compact biosensor chip for clinical diagnosis is presented. The proposed biochip integrated three independent microchannels on one chip partially coated with Au thin film as a surface plasmon resonance (SPR) excitation layer. For clinical diagnosis, the affinity binding of unlabeled biological molecules onto the Au surface can be quantitatively analyzed by SPR imaging with the multi-microchannels, i.e. one biosample and two reference flows to obtain an analytical curve
{"title":"Development of Biosensor Chip for Clinical Diagnosis Using Surface Plasmon Resonance Imaging with Multi-Microchannels","authors":"T. Suzuki, Y. Teramura, K. Inokuma, I. Kanno, H. Iwata, H. Kotera","doi":"10.1109/MMB.2006.251505","DOIUrl":"https://doi.org/10.1109/MMB.2006.251505","url":null,"abstract":"A compact biosensor chip for clinical diagnosis is presented. The proposed biochip integrated three independent microchannels on one chip partially coated with Au thin film as a surface plasmon resonance (SPR) excitation layer. For clinical diagnosis, the affinity binding of unlabeled biological molecules onto the Au surface can be quantitatively analyzed by SPR imaging with the multi-microchannels, i.e. one biosample and two reference flows to obtain an analytical curve","PeriodicalId":170356,"journal":{"name":"2006 International Conference on Microtechnologies in Medicine and Biology","volume":"246 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132027775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
D. C. Ng, T. Tokuda, K. Kagawa, H. Tamura, Sadao Shiosaka, J. Ohta
We have developed a new method for in vivo functional imaging of the mouse brain using a dedicated CMOS image sensor chip. The image sensor has 176times144-pixels with pixel size of 7.5times7.5 mum 2. A novel packaging process is developed to enable on-chip fluorescence imaging. The sensor chip is attached to a flexible polyimide substrate and sealed in epoxy. A thin-film resist is spin-coated directly onto the image sensor chip for excitation light filtering. By applying multiple coating, a transmittance below -44 dB is achieved. Also, the device has a selectivity of more than 80% for the fluorescence emission of 7-amino-4-methylcoumarin (AMC). The entire packaged device is about 350 mum thick, hence minimizing injury during invasive imaging inside the brain. In vivo functional imaging is performed by using a synthetic fluorogenic substrate which detects the presence of two serine proteases species in the brain. The introduction of kainic acid induces the expression of these protease species, which then reacts with the substrate to release the AMC fluorophore. Imaging of the AMC fluorescence allows the serine protease activity to be measured in real-time. We have successfully measured the protease activity and accurately determined its reaction onset
我们开发了一种使用专用CMOS图像传感器芯片对小鼠大脑进行体内功能成像的新方法。图像传感器为176 × 144像素，像素大小为7.5 × 7.5 μ m 2。开发了一种新的封装工艺，以实现片上荧光成像。传感器芯片附着在柔性聚酰亚胺基板上，并用环氧树脂密封。将薄膜抗蚀剂直接自旋涂覆在图像传感器芯片上，用于激发光滤波。通过多次涂布，可实现-44 dB以下的透光率。此外，该装置对7-氨基-4-甲基香豆素(AMC)的荧光发射选择性超过80%。整个包装设备的厚度约为350毫米，因此在脑内侵入性成像时最大限度地减少了损伤。体内功能成像是通过使用合成荧光底物进行的，该底物检测大脑中两种丝氨酸蛋白酶的存在。kainic酸的引入诱导了这些蛋白酶的表达，然后这些蛋白酶与底物反应释放AMC荧光团。AMC荧光成像允许实时测量丝氨酸蛋白酶活性。我们成功地测量了蛋白酶的活性，并准确地确定了它的反应开始
{"title":"On-Chip In vivo Functional Imaging of the Mouse Brain Using a CMOS Image Sensor","authors":"D. C. Ng, T. Tokuda, K. Kagawa, H. Tamura, Sadao Shiosaka, J. Ohta","doi":"10.1109/MMB.2006.251531","DOIUrl":"https://doi.org/10.1109/MMB.2006.251531","url":null,"abstract":"We have developed a new method for in vivo functional imaging of the mouse brain using a dedicated CMOS image sensor chip. The image sensor has 176times144-pixels with pixel size of 7.5times7.5 mum 2. A novel packaging process is developed to enable on-chip fluorescence imaging. The sensor chip is attached to a flexible polyimide substrate and sealed in epoxy. A thin-film resist is spin-coated directly onto the image sensor chip for excitation light filtering. By applying multiple coating, a transmittance below -44 dB is achieved. Also, the device has a selectivity of more than 80% for the fluorescence emission of 7-amino-4-methylcoumarin (AMC). The entire packaged device is about 350 mum thick, hence minimizing injury during invasive imaging inside the brain. In vivo functional imaging is performed by using a synthetic fluorogenic substrate which detects the presence of two serine proteases species in the brain. The introduction of kainic acid induces the expression of these protease species, which then reacts with the substrate to release the AMC fluorophore. Imaging of the AMC fluorescence allows the serine protease activity to be measured in real-time. We have successfully measured the protease activity and accurately determined its reaction onset","PeriodicalId":170356,"journal":{"name":"2006 International Conference on Microtechnologies in Medicine and Biology","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132045441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Determination of cell size is crucial in many biomedical applications. Here, we show how resistive-pulse sensing and artificial pores can be used to detect and measure cell size accurately. Cell size is determined by measuring the change in resistance when an individual cell passes through the pore. As a proof-of-principle, we show that we are able to measure the change in size when cells undergo apoptosis
{"title":"The NanoCytometer: Screening Cells Based on Cell Size","authors":"A. Carbonaro, L. Godley, L. Sohn","doi":"10.1109/MMB.2006.251529","DOIUrl":"https://doi.org/10.1109/MMB.2006.251529","url":null,"abstract":"Determination of cell size is crucial in many biomedical applications. Here, we show how resistive-pulse sensing and artificial pores can be used to detect and measure cell size accurately. Cell size is determined by measuring the change in resistance when an individual cell passes through the pore. As a proof-of-principle, we show that we are able to measure the change in size when cells undergo apoptosis","PeriodicalId":170356,"journal":{"name":"2006 International Conference on Microtechnologies in Medicine and Biology","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122623001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
T. Roussel, R. Pai, M. Grain, D. Jackson, L. Sztaberek, K. Walsh, J. Naber, R. Baldwin, R. Keynton
This paper presents results indicating the complete oxidation of an analyte using a three-dimensional, large surface area microelectrode in a lab-on-a-chip (LOC) device. Traditional photolithographic fabrication techniques were used to construct the microchip prototype, with the exception of the 3D electrodes. Experiments were performed using a flow-injection scheme, where a 1 mM sample of catechol was directed towards the detection reservoir. Oxidation voltage at a planar upstream electrode was increased from 0 to 1.2 V in 0.1 volt increments and was shown to reduce the detection level of the sample at a downstream planar electrode (1.2 V). Oxidation voltage at the upstream 3D electrode was changed in a similar manner and was shown to completely eliminate detection at a downstream planar electrode. These results indicate the complete removal of sample from the flow stream, suggesting that 3D micro fabricated electrodes could be used in an LOC device for coulometric screening
{"title":"3D Microelectrodes for Coulometric Screening in Microfabricated Lab-on-a-Chip Devices","authors":"T. Roussel, R. Pai, M. Grain, D. Jackson, L. Sztaberek, K. Walsh, J. Naber, R. Baldwin, R. Keynton","doi":"10.1109/MMB.2006.251537","DOIUrl":"https://doi.org/10.1109/MMB.2006.251537","url":null,"abstract":"This paper presents results indicating the complete oxidation of an analyte using a three-dimensional, large surface area microelectrode in a lab-on-a-chip (LOC) device. Traditional photolithographic fabrication techniques were used to construct the microchip prototype, with the exception of the 3D electrodes. Experiments were performed using a flow-injection scheme, where a 1 mM sample of catechol was directed towards the detection reservoir. Oxidation voltage at a planar upstream electrode was increased from 0 to 1.2 V in 0.1 volt increments and was shown to reduce the detection level of the sample at a downstream planar electrode (1.2 V). Oxidation voltage at the upstream 3D electrode was changed in a similar manner and was shown to completely eliminate detection at a downstream planar electrode. These results indicate the complete removal of sample from the flow stream, suggesting that 3D micro fabricated electrodes could be used in an LOC device for coulometric screening","PeriodicalId":170356,"journal":{"name":"2006 International Conference on Microtechnologies in Medicine and Biology","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115090210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. Takano, L. Doeswijk, M. van den Boogaart, J. Brugger
Microstencil lithography, a resistless, single-step direct vacuum patterning method, is one of promising methods for metal micropattern definition on polymer substrates that are not suitable for conventional photolithography. We propose to apply microstencil lithography to fabricate microelectrodes on flat and pre-structured polymer substrates which form parts of microfluidic systems with incorporated microelectrodes. However, microstencil lithography is accompanied by two main issues when considered as a low-cost, reproducible alternative to standard photolithography on polymer substrates: clogging and blurring. The clogging of stencil apertures induced by metal evaporation was checked in detail, and it was determined that approximately 50 % of the thickness of the evaporated metals was deposited at the side walls of the stencil apertures. The influence of gap presence on the deposited structures was also analyzed experimentally, and we quantified the pattern blurring
{"title":"Application of Microstencil Lithography on Polymer Surfaces for Microfluidic Systems with Integrated Microelectrodes","authors":"N. Takano, L. Doeswijk, M. van den Boogaart, J. Brugger","doi":"10.1109/MMB.2006.251511","DOIUrl":"https://doi.org/10.1109/MMB.2006.251511","url":null,"abstract":"Microstencil lithography, a resistless, single-step direct vacuum patterning method, is one of promising methods for metal micropattern definition on polymer substrates that are not suitable for conventional photolithography. We propose to apply microstencil lithography to fabricate microelectrodes on flat and pre-structured polymer substrates which form parts of microfluidic systems with incorporated microelectrodes. However, microstencil lithography is accompanied by two main issues when considered as a low-cost, reproducible alternative to standard photolithography on polymer substrates: clogging and blurring. The clogging of stencil apertures induced by metal evaporation was checked in detail, and it was determined that approximately 50 % of the thickness of the evaporated metals was deposited at the side walls of the stencil apertures. The influence of gap presence on the deposited structures was also analyzed experimentally, and we quantified the pattern blurring","PeriodicalId":170356,"journal":{"name":"2006 International Conference on Microtechnologies in Medicine and Biology","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127402497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This paper describes a three dimensional (3D) finite element model of micromachined stimulating microelectrode arrays for cortical stimulation. These micromachined probes, known as Michigan probes, are lithographically defined in geometry and fabricated though mostly standard silicon processing technology. However, the fabrication process requires the highly conductive boron-doped shanks, which provide mechanical support for the electrode array, to be grounded especially in active electrodes that incorporate integrated circuits on the same chip. We have examined the effects of grounding this portion (body) of the electrode arrays using finite element analysis (FEA) and drawn a few conclusions about their design. Further, using information gained from our FEA models, we evaluate the current distribution and volume of the excited tissue in a proposed design that would increase the density of electrodes in a given volume and provides greater precision for targeted stimulation
{"title":"Finite Element Analysis of Planar Micromachined Silicon Electrodes for Cortical Stimulation","authors":"R. Field, Maysam Ghovanloo","doi":"10.1109/MMB.2006.251533","DOIUrl":"https://doi.org/10.1109/MMB.2006.251533","url":null,"abstract":"This paper describes a three dimensional (3D) finite element model of micromachined stimulating microelectrode arrays for cortical stimulation. These micromachined probes, known as Michigan probes, are lithographically defined in geometry and fabricated though mostly standard silicon processing technology. However, the fabrication process requires the highly conductive boron-doped shanks, which provide mechanical support for the electrode array, to be grounded especially in active electrodes that incorporate integrated circuits on the same chip. We have examined the effects of grounding this portion (body) of the electrode arrays using finite element analysis (FEA) and drawn a few conclusions about their design. Further, using information gained from our FEA models, we evaluate the current distribution and volume of the excited tissue in a proposed design that would increase the density of electrodes in a given volume and provides greater precision for targeted stimulation","PeriodicalId":170356,"journal":{"name":"2006 International Conference on Microtechnologies in Medicine and Biology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131222751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This paper reports the characterization of adhesion-based cell velocity regulation in a prototype microfabricated cell separation device for regenerative medicine. The principle of cell sorting is based on immunoreaction for accurate recognition of target stem cells. Target-cell specific antibody is immobilized on the micro channel wall to form a selectively adhesive surface, where a new class of functionalized parylene is used as the surface material for antibody immobilization. The flowing velocity of sample cells in a prototype microfabricated cell separation column is examined under the microscope. The measurement results show that the cell velocity is reduced by 40% due to the effect of antigen/antibody interaction
{"title":"Evaluation of Cell Velocity Regulation in a Microfabricated Adhesion-Based Cell Separation Device","authors":"J. Miwa, Y. Suzuki, N. Kasagi","doi":"10.1109/MMB.2006.251501","DOIUrl":"https://doi.org/10.1109/MMB.2006.251501","url":null,"abstract":"This paper reports the characterization of adhesion-based cell velocity regulation in a prototype microfabricated cell separation device for regenerative medicine. The principle of cell sorting is based on immunoreaction for accurate recognition of target stem cells. Target-cell specific antibody is immobilized on the micro channel wall to form a selectively adhesive surface, where a new class of functionalized parylene is used as the surface material for antibody immobilization. The flowing velocity of sample cells in a prototype microfabricated cell separation column is examined under the microscope. The measurement results show that the cell velocity is reduced by 40% due to the effect of antigen/antibody interaction","PeriodicalId":170356,"journal":{"name":"2006 International Conference on Microtechnologies in Medicine and Biology","volume":"32 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131356404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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