We report a new microfluidic system, `lab-on-a-display', that microparticles can be manipulated by dielectrophoretic forces generated from the optoelectronic tweezers (OET) on a liquid crystal display (LCD). In this study, the lab-on-a-display is first realized by a conventional LCD module and an OET device including a ground layer, a liquid layer containing microparticles, and a photoconductive layer. When an AC bias voltage is applied between the photoconductive and the ground layers, the LCD makes an image and transmits it to the OET device. Consequently, the image forms virtual electrodes on the surface of photoconductive layer, which results in the electric field gradient to generate a dielectrophoretic force. The lab-on-a-display is successfully applied to the programmable manipulation of 45 mum polystyrene particles. Our lab-on-a-display platform may be potential for programmable particle manipulation or a bioprocessing unit including a cell separator and a bead-based immunoassay
{"title":"Lab-on-a-Display: Microparticles Manipulation using Liquid Crystal Display","authors":"W. Choi, Sewhan Kim, Jin Jang, J. Park","doi":"10.1109/MMB.2006.251484","DOIUrl":"https://doi.org/10.1109/MMB.2006.251484","url":null,"abstract":"We report a new microfluidic system, `lab-on-a-display', that microparticles can be manipulated by dielectrophoretic forces generated from the optoelectronic tweezers (OET) on a liquid crystal display (LCD). In this study, the lab-on-a-display is first realized by a conventional LCD module and an OET device including a ground layer, a liquid layer containing microparticles, and a photoconductive layer. When an AC bias voltage is applied between the photoconductive and the ground layers, the LCD makes an image and transmits it to the OET device. Consequently, the image forms virtual electrodes on the surface of photoconductive layer, which results in the electric field gradient to generate a dielectrophoretic force. The lab-on-a-display is successfully applied to the programmable manipulation of 45 mum polystyrene particles. Our lab-on-a-display platform may be potential for programmable particle manipulation or a bioprocessing unit including a cell separator and a bead-based immunoassay","PeriodicalId":170356,"journal":{"name":"2006 International Conference on Microtechnologies in Medicine and Biology","volume":"35 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115240782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In this study an integrated in situ analyzer system for gene analysis (IISA-Gene) is developed to perform gene analysis of microorganisms inhabiting in extreme environments such as deep-sea or deep subsurface. Microfabricated chips made of PDMS and glass is employed to achieve a totally miniaturized and integrated system. Bacterial cell lysis, DNA purification, PCR amplification and amplified fragment detection can be performed with IISA-Gene system including miniaturized pumping and optical detection device. The IISA-Gene developed in this study was evaluated and operated in real deep-sea environment
{"title":"Development and Evaluation of the Integrated In Situ Analyzer for Gene-\"IISA-Gene\" for Microbiology in Extreme Environments","authors":"T. Fukuba, T. Fujii","doi":"10.1109/MMB.2006.251523","DOIUrl":"https://doi.org/10.1109/MMB.2006.251523","url":null,"abstract":"In this study an integrated in situ analyzer system for gene analysis (IISA-Gene) is developed to perform gene analysis of microorganisms inhabiting in extreme environments such as deep-sea or deep subsurface. Microfabricated chips made of PDMS and glass is employed to achieve a totally miniaturized and integrated system. Bacterial cell lysis, DNA purification, PCR amplification and amplified fragment detection can be performed with IISA-Gene system including miniaturized pumping and optical detection device. The IISA-Gene developed in this study was evaluated and operated in real deep-sea environment","PeriodicalId":170356,"journal":{"name":"2006 International Conference on Microtechnologies in Medicine and Biology","volume":"81 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124119519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Kumemura, K. Tamura, G. Hashiguchi, D. Collard, H. Fujita
Micromachined tweezers is having nanometer sized gap was fabricated with a silicon etching method and a focussed ion beam technique. The gap of tweezer fabricated by this process was accomplished to be 15 nm-2 mum ranges. The validity of this tweezers was demonstrated by trapping DNA molecules. Trapping of bundle of lambda-DNA molecules between 100 mum gap was succeeded
{"title":"Nano-gap fabrication by focused ion beam for DNA trapping","authors":"M. Kumemura, K. Tamura, G. Hashiguchi, D. Collard, H. Fujita","doi":"10.1109/MMB.2006.251547","DOIUrl":"https://doi.org/10.1109/MMB.2006.251547","url":null,"abstract":"Micromachined tweezers is having nanometer sized gap was fabricated with a silicon etching method and a focussed ion beam technique. The gap of tweezer fabricated by this process was accomplished to be 15 nm-2 mum ranges. The validity of this tweezers was demonstrated by trapping DNA molecules. Trapping of bundle of lambda-DNA molecules between 100 mum gap was succeeded","PeriodicalId":170356,"journal":{"name":"2006 International Conference on Microtechnologies in Medicine and Biology","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128462729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
To improve diffusive capabilities and target accessibility with a gel-based microarray, a novel `pads within a pad' approach was developed. Using a soft lithography technique, an array of `waffle'-like micro-pillars embedded on the surface of the glass slide substratum was fabricated. The micro-pillar based microarray showed more than a 3-fold increase in the effective surface area available for probe immobilization. As a result, a fivefold increase was observed in the hybridization rate and intensity of the `waffle' pad as compared to an unmodified gel pad. The functionality of the `waffle' pad was demonstrated by the accurate genotyping of two sets of SNPs (SNP_ 1236 and SNP_2677) based on the dissociation curve, dissociation temperature, Td and the difference of Td between the perfect match duplex with that of the mismatch (DeltaTd)
{"title":"Polyacrylamide micro-pillar based DNA microarray and its application for genetic variation analysis.","authors":"E. S. Selamat, P.Y. Hong, W. Liu","doi":"10.1109/MMB.2006.251549","DOIUrl":"https://doi.org/10.1109/MMB.2006.251549","url":null,"abstract":"To improve diffusive capabilities and target accessibility with a gel-based microarray, a novel `pads within a pad' approach was developed. Using a soft lithography technique, an array of `waffle'-like micro-pillars embedded on the surface of the glass slide substratum was fabricated. The micro-pillar based microarray showed more than a 3-fold increase in the effective surface area available for probe immobilization. As a result, a fivefold increase was observed in the hybridization rate and intensity of the `waffle' pad as compared to an unmodified gel pad. The functionality of the `waffle' pad was demonstrated by the accurate genotyping of two sets of SNPs (SNP_ 1236 and SNP_2677) based on the dissociation curve, dissociation temperature, Td and the difference of Td between the perfect match duplex with that of the mismatch (DeltaTd)","PeriodicalId":170356,"journal":{"name":"2006 International Conference on Microtechnologies in Medicine and Biology","volume":"16 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128693493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
W. Li, D. Rodger, E. Meng, J. Weiland, M. Humayun, Y. Tai
We present a flexible and fully-implantable coil designed for use as a power and data transfer component in retinal prosthesis applications. Compared with traditional hand-made intraocular coils, this microfabricated coil is flexible, with a 9.5 mm outer diameter and 10-mum-thick parylene C as the primary structural and packaging material. A post-fabrication heat treatment was used to improve the parylene package in order to protect the device in harsh corrosive environments such as the human eye. Long-term accelerated-lifetime soak testing in heated saline has been performed, and the mean time to failure (MTTF) of the parylene package extrapolated to 37degC was estimated using the Arrhenius relationship. The electrical failure of this device was also characterized by measuring the DC resistance in saline
{"title":"Flexible Parylene Packaged Intraocular Coil for Retinal Prostheses","authors":"W. Li, D. Rodger, E. Meng, J. Weiland, M. Humayun, Y. Tai","doi":"10.1109/MMB.2006.251502","DOIUrl":"https://doi.org/10.1109/MMB.2006.251502","url":null,"abstract":"We present a flexible and fully-implantable coil designed for use as a power and data transfer component in retinal prosthesis applications. Compared with traditional hand-made intraocular coils, this microfabricated coil is flexible, with a 9.5 mm outer diameter and 10-mum-thick parylene C as the primary structural and packaging material. A post-fabrication heat treatment was used to improve the parylene package in order to protect the device in harsh corrosive environments such as the human eye. Long-term accelerated-lifetime soak testing in heated saline has been performed, and the mean time to failure (MTTF) of the parylene package extrapolated to 37degC was estimated using the Arrhenius relationship. The electrical failure of this device was also characterized by measuring the DC resistance in saline","PeriodicalId":170356,"journal":{"name":"2006 International Conference on Microtechnologies in Medicine and Biology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130618641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
We report a method for forming ultra giant lipid vesicles. A chip device with a micro-jet nozzle was fabricated. The vesicles were produced by applying pulsed micro jets to a planar lipid bilayer, analogous to blowing soap bubbles from a liquid film. Using this method, lipid vesicles with diameters of about 500 mum were formed one-by-one. This method enables the encapsulation of target molecules or nano particles into the vesicles rapidly and efficiently
{"title":"Ultra Giant Vesicles out of a Planar Membrane","authors":"K. Funakoshi, H. Suzuki, S. Takeuchi","doi":"10.1109/MMB.2006.251492","DOIUrl":"https://doi.org/10.1109/MMB.2006.251492","url":null,"abstract":"We report a method for forming ultra giant lipid vesicles. A chip device with a micro-jet nozzle was fabricated. The vesicles were produced by applying pulsed micro jets to a planar lipid bilayer, analogous to blowing soap bubbles from a liquid film. Using this method, lipid vesicles with diameters of about 500 mum were formed one-by-one. This method enables the encapsulation of target molecules or nano particles into the vesicles rapidly and efficiently","PeriodicalId":170356,"journal":{"name":"2006 International Conference on Microtechnologies in Medicine and Biology","volume":"52 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127902352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
P. Mulder, G. Molema, S. Koster, H.J. van der Linden, E. Verpoorte
Our long-term goal is to develop advanced tools for cell studies and analysis based on microfluidic systems. In this paper, we report on endothelial cell cultivation in microchannels and 96-well tissue plates, and compare cell phenotype and cellular status in the two environments This was done under both pro-inflammatory conditions (cell stimulation in the presence of the cytokine, Tumour Necrosis Factor, TNFalpha) and normal (medium only) conditions. In addition, we considered the behaviour of cells in a pro-inflammatory environment in the presence of an anti-inflammatory drug
{"title":"Behaviour of Human Umbilical Vein Endothelial Cells (HUVEC) Cultivated in Microfluidic channels","authors":"P. Mulder, G. Molema, S. Koster, H.J. van der Linden, E. Verpoorte","doi":"10.1109/MMB.2006.251515","DOIUrl":"https://doi.org/10.1109/MMB.2006.251515","url":null,"abstract":"Our long-term goal is to develop advanced tools for cell studies and analysis based on microfluidic systems. In this paper, we report on endothelial cell cultivation in microchannels and 96-well tissue plates, and compare cell phenotype and cellular status in the two environments This was done under both pro-inflammatory conditions (cell stimulation in the presence of the cytokine, Tumour Necrosis Factor, TNFalpha) and normal (medium only) conditions. In addition, we considered the behaviour of cells in a pro-inflammatory environment in the presence of an anti-inflammatory drug","PeriodicalId":170356,"journal":{"name":"2006 International Conference on Microtechnologies in Medicine and Biology","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126445244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
G. Kwon, J. Baek, Seung-Ha Lee, M. Chang, Dong-Cho Lee, Kyung-Sun, Sang-Hoon Lee
This paper presents a polydimethylsiloxane (PDMS)-based flexible and implantable micro electrode for the retinal prosthesis. The patterning of the electrode and the wires was performed through the photolithography and chemical etching process after e-beam deposition Ti and Au, and the thick electrode was fabricated through the electroplating process. We carried out in-vivo test for the evaluation of biocompatibility by implanting electrodes under the ICR mouse skin for 45 days. The electrode was implanted under the retina of rabbit's eye to investigate the feasibility as a retinal prosthesis
{"title":"Fabrication and Evaluation of the Flexible and Implantable Soft Micro Electrode for Retinal Prosthesis","authors":"G. Kwon, J. Baek, Seung-Ha Lee, M. Chang, Dong-Cho Lee, Kyung-Sun, Sang-Hoon Lee","doi":"10.1109/MMB.2006.251524","DOIUrl":"https://doi.org/10.1109/MMB.2006.251524","url":null,"abstract":"This paper presents a polydimethylsiloxane (PDMS)-based flexible and implantable micro electrode for the retinal prosthesis. The patterning of the electrode and the wires was performed through the photolithography and chemical etching process after e-beam deposition Ti and Au, and the thick electrode was fabricated through the electroplating process. We carried out in-vivo test for the evaluation of biocompatibility by implanting electrodes under the ICR mouse skin for 45 days. The electrode was implanted under the retina of rabbit's eye to investigate the feasibility as a retinal prosthesis","PeriodicalId":170356,"journal":{"name":"2006 International Conference on Microtechnologies in Medicine and Biology","volume":"103 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116209263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
We describe the design and manufacture of a thin film thermo-couple (TFTC) which is intended for real time observations of temperature-dependant phenomena of biological systems. The TFTC design aims at shortening its response time. The TFTC manufacturing process combines platinum, silica and chromium sputtering with reactive ion etching (RIE) on glass substrate. Our device consists of a platinum wire (the heater) near a micro TFTC sensor. This sensor consists in two wires made of platinum and chromium. These wires are in contact on a 2times2 mum area. Our micro TFTC shows a simulated response time of 0.35 ms when used in air (for DeltaTap55 K), and 1.5 ms when used in water (for DeltaTap41 K)
{"title":"High response time micro scale thermo-couple for biological applications","authors":"F. Gillot, A. Tixier-Mita, F. Morin, H. Fujita","doi":"10.1109/MMB.2006.251508","DOIUrl":"https://doi.org/10.1109/MMB.2006.251508","url":null,"abstract":"We describe the design and manufacture of a thin film thermo-couple (TFTC) which is intended for real time observations of temperature-dependant phenomena of biological systems. The TFTC design aims at shortening its response time. The TFTC manufacturing process combines platinum, silica and chromium sputtering with reactive ion etching (RIE) on glass substrate. Our device consists of a platinum wire (the heater) near a micro TFTC sensor. This sensor consists in two wires made of platinum and chromium. These wires are in contact on a 2times2 mum area. Our micro TFTC shows a simulated response time of 0.35 ms when used in air (for DeltaTap55 K), and 1.5 ms when used in water (for DeltaTap41 K)","PeriodicalId":170356,"journal":{"name":"2006 International Conference on Microtechnologies in Medicine and Biology","volume":"110 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121591618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
C. Chong, K. Isamoto, M. Nakada, H. Fujita, H. Toshiyoshi
We have developed a new SiP (system in package) style optical MEMS (microelectromechanical system) component for fiber optic endoscope that operates without using external power supply. The endoscope sensor head (5 mm in outer diameter) attached at the end of an optical fiber is designed for the use of in vivo medical diagnosis such as gastrointestinal tact or vascular vein monitor by using an infrared probe light at a 1.3-micron-wavelength. Another infrared light of at a 1.5-micron-wavelength is used in the identical optics in a WDM (wavelength division multiplex) manner to generate the drive voltage (of up to 5 V) in the package by using a collocated photovoltaic cell. This paper describes the architecture of the optical actuation system, the scanner design and fabrication. Modulation of the MEMS scanner is demonstrated using the optical modulation, by which the risk of electrocution or electromagnetic interference is avoided
{"title":"A Photovoltaically Modulated MEMS Optical Scanner for Fiber Endoscope","authors":"C. Chong, K. Isamoto, M. Nakada, H. Fujita, H. Toshiyoshi","doi":"10.1109/MMB.2006.251510","DOIUrl":"https://doi.org/10.1109/MMB.2006.251510","url":null,"abstract":"We have developed a new SiP (system in package) style optical MEMS (microelectromechanical system) component for fiber optic endoscope that operates without using external power supply. The endoscope sensor head (5 mm in outer diameter) attached at the end of an optical fiber is designed for the use of in vivo medical diagnosis such as gastrointestinal tact or vascular vein monitor by using an infrared probe light at a 1.3-micron-wavelength. Another infrared light of at a 1.5-micron-wavelength is used in the identical optics in a WDM (wavelength division multiplex) manner to generate the drive voltage (of up to 5 V) in the package by using a collocated photovoltaic cell. This paper describes the architecture of the optical actuation system, the scanner design and fabrication. Modulation of the MEMS scanner is demonstrated using the optical modulation, by which the risk of electrocution or electromagnetic interference is avoided","PeriodicalId":170356,"journal":{"name":"2006 International Conference on Microtechnologies in Medicine and Biology","volume":"88 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134409181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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