S. Goto, T. Matsunaga, J.J. Chen, W. Makishi, M. Esashi, Y. Haga
This paper reports fabrication techniques for multilayer microstructures and surface mounting of small components on cylindrical substrates for tube-shaped high-performance micro-tools. A cylindrical shape is an ideal shape for micro medical tools inserted temporarily or implanted in the human body. Miniaturization, multifunctional capabilities, and high-performance are required for these tools. Tubular lumens, which are useful for the insertion of medical tools and for the injection of drugs, are necessary for endoscopes and catheters. Micro-fabrication on cylindrical substrates meets these demands. Multilayer metallization and patterning, as well as three-dimensional resist patterning have been performed using maskless lithography techniques on glass tubes with 2 and 3 mm outer diameters. Using laser soldering techniques, a high-speed OP amp has been mounted on a multilayer circuit formed on a glass tube to amplify small signals from micro medical sensors. These techniques will realize multifunctional and high-performance tube-shaped micro medical tools with small diameters
{"title":"Fabrication Techniques for Multilayer Metalization and Patterning, and Surface Mounting of Components on Cylindrical Substrates for Tube-Shaped Micro-Tools","authors":"S. Goto, T. Matsunaga, J.J. Chen, W. Makishi, M. Esashi, Y. Haga","doi":"10.1109/MMB.2006.251532","DOIUrl":"https://doi.org/10.1109/MMB.2006.251532","url":null,"abstract":"This paper reports fabrication techniques for multilayer microstructures and surface mounting of small components on cylindrical substrates for tube-shaped high-performance micro-tools. A cylindrical shape is an ideal shape for micro medical tools inserted temporarily or implanted in the human body. Miniaturization, multifunctional capabilities, and high-performance are required for these tools. Tubular lumens, which are useful for the insertion of medical tools and for the injection of drugs, are necessary for endoscopes and catheters. Micro-fabrication on cylindrical substrates meets these demands. Multilayer metallization and patterning, as well as three-dimensional resist patterning have been performed using maskless lithography techniques on glass tubes with 2 and 3 mm outer diameters. Using laser soldering techniques, a high-speed OP amp has been mounted on a multilayer circuit formed on a glass tube to amplify small signals from micro medical sensors. These techniques will realize multifunctional and high-performance tube-shaped micro medical tools with small diameters","PeriodicalId":170356,"journal":{"name":"2006 International Conference on Microtechnologies in Medicine and Biology","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133853931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Interaction of shock waves with renal tumor cell line (ACHN) has been studied with regard to their membrane micro-filament structure as a major cellular system to resist mechanical stress. ACHN monolayer cultured on a cover slide glass was treated with 16 MPa peak pressure focused underwater shock waves. The morphological deformations were found to be associated with disorganization of the intracellular cytoskeletal filaments. In order to simulate the interaction of shock waves with fibrillar network structure of cells, thin porous layers of cotton immersed in water were exposed to underwater shock waves. Attenuation of shock over-pressures were measured with needle and fiber optic probe hydrophones. The motion of shock waves was quantitatively visualized. The experimental results were extended to understand the complex process of shock/cell interactions, which would happen during ESW cancer therapy
{"title":"Shock Wave Interaction with Micro-Filament Network in a Cancer Ce1l line","authors":"S. Hosseini, S. Moosavi-Nejad, K. Takayama","doi":"10.1109/MMB.2006.251534","DOIUrl":"https://doi.org/10.1109/MMB.2006.251534","url":null,"abstract":"Interaction of shock waves with renal tumor cell line (ACHN) has been studied with regard to their membrane micro-filament structure as a major cellular system to resist mechanical stress. ACHN monolayer cultured on a cover slide glass was treated with 16 MPa peak pressure focused underwater shock waves. The morphological deformations were found to be associated with disorganization of the intracellular cytoskeletal filaments. In order to simulate the interaction of shock waves with fibrillar network structure of cells, thin porous layers of cotton immersed in water were exposed to underwater shock waves. Attenuation of shock over-pressures were measured with needle and fiber optic probe hydrophones. The motion of shock waves was quantitatively visualized. The experimental results were extended to understand the complex process of shock/cell interactions, which would happen during ESW cancer therapy","PeriodicalId":170356,"journal":{"name":"2006 International Conference on Microtechnologies in Medicine and Biology","volume":"23 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134045067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. Lo, K. Kuwahara, P.-Y. Li, R. Agrawal, M. Humayun, E. Meng
This paper presents the first passive implantable microelectromechanical systems (MEMS) device for targeted intraocular delivery of therapeutic compounds. In particular, this device addresses the treatment of chronic, difficult to reach diseases that affect the retina including retinitis pigmentosa, age-related macular degeneration, diabetic retinopathy, and glaucoma. The device is composed of three structural polymethyldisiloxane (PDMS) layers that are irreversibly bonded without the use of any adhesives. These layers form an integrated drug delivery device consisting of a refillable reservoir, tube, check valve, and suture tabs. This device requires a single implantation surgery and is capable of repeated delivery of multiple drugs. Characterization of the refillable reservoir and check valve performance is presented. Preliminary surgical implantation results of a mechanical test structure are also presented
{"title":"A Passive Refillable Intraocular MEMS Drug Delivery Device","authors":"R. Lo, K. Kuwahara, P.-Y. Li, R. Agrawal, M. Humayun, E. Meng","doi":"10.1109/MMB.2006.251494","DOIUrl":"https://doi.org/10.1109/MMB.2006.251494","url":null,"abstract":"This paper presents the first passive implantable microelectromechanical systems (MEMS) device for targeted intraocular delivery of therapeutic compounds. In particular, this device addresses the treatment of chronic, difficult to reach diseases that affect the retina including retinitis pigmentosa, age-related macular degeneration, diabetic retinopathy, and glaucoma. The device is composed of three structural polymethyldisiloxane (PDMS) layers that are irreversibly bonded without the use of any adhesives. These layers form an integrated drug delivery device consisting of a refillable reservoir, tube, check valve, and suture tabs. This device requires a single implantation surgery and is capable of repeated delivery of multiple drugs. Characterization of the refillable reservoir and check valve performance is presented. Preliminary surgical implantation results of a mechanical test structure are also presented","PeriodicalId":170356,"journal":{"name":"2006 International Conference on Microtechnologies in Medicine and Biology","volume":"144 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116434457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
We report a new approach for micro Coulter counter with thin film electrodes. Traditional Coulter counter measures DC resistance change to sense particles passing through an aperture between two flow chambers. One of the biggest challenges to miniaturize the device is to overcome the large resistance encountered. We propose a new approach to use thin film metal electrodes in micron size range for sensing. Resolution can be improved and it's easy for system integration. A device based on this approach was built and tested
{"title":"Design and Fabrication of a Micro Coulter Counter with Thin Film Electrodes","authors":"Si-Yang Zheng, Y. Tai","doi":"10.1109/MMB.2006.251479","DOIUrl":"https://doi.org/10.1109/MMB.2006.251479","url":null,"abstract":"We report a new approach for micro Coulter counter with thin film electrodes. Traditional Coulter counter measures DC resistance change to sense particles passing through an aperture between two flow chambers. One of the biggest challenges to miniaturize the device is to overcome the large resistance encountered. We propose a new approach to use thin film metal electrodes in micron size range for sensing. Resolution can be improved and it's easy for system integration. A device based on this approach was built and tested","PeriodicalId":170356,"journal":{"name":"2006 International Conference on Microtechnologies in Medicine and Biology","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121737743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J. Mizuno, H. Nakamura, Y. Murayama, S. Omata, H. Ando, K. Akaishi, N. Watanabe, K. Hirayama, E. Kuriki, A. Watanabe, H. Inui
In vitro fertilization (IVF) and in vitro culture (IVC) are important technics for human ART. Especially, IVC is most important factor to achieve good/high pregnancy rate. In order to reach this goal, we have to develop in vivo mimic/like human embryo co-culture system. We presented/introduced a new stretch embryo co-culture system for human ART
{"title":"New embryo co-culture system for human Assisted Reproductive Technology (ART): Verification of the effects of mechanical stress to embryo co-culture system","authors":"J. Mizuno, H. Nakamura, Y. Murayama, S. Omata, H. Ando, K. Akaishi, N. Watanabe, K. Hirayama, E. Kuriki, A. Watanabe, H. Inui","doi":"10.1109/MMB.2006.251485","DOIUrl":"https://doi.org/10.1109/MMB.2006.251485","url":null,"abstract":"In vitro fertilization (IVF) and in vitro culture (IVC) are important technics for human ART. Especially, IVC is most important factor to achieve good/high pregnancy rate. In order to reach this goal, we have to develop in vivo mimic/like human embryo co-culture system. We presented/introduced a new stretch embryo co-culture system for human ART","PeriodicalId":170356,"journal":{"name":"2006 International Conference on Microtechnologies in Medicine and Biology","volume":"189 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124186606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
We have successfully demonstrated a microfluidic flow-focusing method to produce monodisperse contrast agents with a mean diameter of 4.0 microns, which is optimal for current ultrasonic imaging techniques. Currently available microbubble contrast agents are produced with agitation techniques, and the random nature of this process results in a highly polydisperse size distribution. PDMS-based microfluidic flow-focusing systems are a cost-effective means to easily and rapidly produce bubbles with a diameter of a few microns. Our device uses expanding nozzle geometry, focusing the bubble break-off location to one single point located at the orifice, which enables the formation of monodisperse bubbles. The geometry of the channel junctions in addition to the liquid and gas flow rates are used to control the bubble sizes. Using this technique, the microbubble diameter can be easily tailored to produce contrast agents with a precise size distribution optimized for various ultrasonic imaging applications
{"title":"Formulation of Monodisperse Contrast Agents in Microfluidic Systems for Ultrasonic Imaging","authors":"K. Hettiarachchi, P. Dayton, A.P. Lee","doi":"10.1109/MMB.2006.251536","DOIUrl":"https://doi.org/10.1109/MMB.2006.251536","url":null,"abstract":"We have successfully demonstrated a microfluidic flow-focusing method to produce monodisperse contrast agents with a mean diameter of 4.0 microns, which is optimal for current ultrasonic imaging techniques. Currently available microbubble contrast agents are produced with agitation techniques, and the random nature of this process results in a highly polydisperse size distribution. PDMS-based microfluidic flow-focusing systems are a cost-effective means to easily and rapidly produce bubbles with a diameter of a few microns. Our device uses expanding nozzle geometry, focusing the bubble break-off location to one single point located at the orifice, which enables the formation of monodisperse bubbles. The geometry of the channel junctions in addition to the liquid and gas flow rates are used to control the bubble sizes. Using this technique, the microbubble diameter can be easily tailored to produce contrast agents with a precise size distribution optimized for various ultrasonic imaging applications","PeriodicalId":170356,"journal":{"name":"2006 International Conference on Microtechnologies in Medicine and Biology","volume":"72 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129418907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A non-viral and efficient carrier for the applications delivering drugs, plasmic DNA, shRNA for pharmaceutical and therapeutic applications is presented. The cationic lipid based liposomal carriers which has been the most attractive non-viral solution. The size of complexes of cationic lipid and DNA (lipoplex) dictates the efficiency of gene transfection. Moreover, the order of mixing of cationic lipid and DNA, mixing rate, and mixture incubation time are factors that determine the lipoplex sizes during sample preparation. We developed a Picoliter Micro Reactor and Incubator (PMRI) system to formulate monodisperse cationic lipids and DNA complexes. The monodisperse micro reactor and incubator was designed and fabricated based on the microfluidic droplet formation devices which developed by our lab and others. Compared with the conventional irreproducible lipoplex preparation using hand-shaking or vortexing processes for mixing, the PMRI system we developed for lipoplex formulation demonstrates the ability of rapid and uniformly mix cationic lipid and DNA simultaneously, and generates a narrower size distribution peak and smaller average size. The PMRI also enables the mixing and incubation integrated process and lipolex formulation reproducibility
{"title":"Monodisperse Lipoplex Generation by Integrated Picoliter Micro Reactor and Incubator","authors":"A. Hsieh, A. Lin, A.P. Lee","doi":"10.1109/MMB.2006.251516","DOIUrl":"https://doi.org/10.1109/MMB.2006.251516","url":null,"abstract":"A non-viral and efficient carrier for the applications delivering drugs, plasmic DNA, shRNA for pharmaceutical and therapeutic applications is presented. The cationic lipid based liposomal carriers which has been the most attractive non-viral solution. The size of complexes of cationic lipid and DNA (lipoplex) dictates the efficiency of gene transfection. Moreover, the order of mixing of cationic lipid and DNA, mixing rate, and mixture incubation time are factors that determine the lipoplex sizes during sample preparation. We developed a Picoliter Micro Reactor and Incubator (PMRI) system to formulate monodisperse cationic lipids and DNA complexes. The monodisperse micro reactor and incubator was designed and fabricated based on the microfluidic droplet formation devices which developed by our lab and others. Compared with the conventional irreproducible lipoplex preparation using hand-shaking or vortexing processes for mixing, the PMRI system we developed for lipoplex formulation demonstrates the ability of rapid and uniformly mix cationic lipid and DNA simultaneously, and generates a narrower size distribution peak and smaller average size. The PMRI also enables the mixing and incubation integrated process and lipolex formulation reproducibility","PeriodicalId":170356,"journal":{"name":"2006 International Conference on Microtechnologies in Medicine and Biology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124353479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The present paper describes the use of indium-tin oxide microelectrodes for manipulating microtubules in solution through interaction with electric fields. The experimental set-up that is described here is a platform for both investigating the fundamental dielectric properties of microtubules and developing methods to exert control over their behaviour in solution. Direct applications of such methods can be found in the development of microsystems integrating nano-machines based on motor proteins such as kinesin or dynein. Electro-rotation of microtubules in solution is presented as an example of interaction between microtubules and electric field
{"title":"Probing and using the dielectric properties of microtubules within microsystems integrating Indium-Tin oxide microelectrodes","authors":"F. Morin, M. Tarhan, H. Fujita","doi":"10.1109/MMB.2006.251489","DOIUrl":"https://doi.org/10.1109/MMB.2006.251489","url":null,"abstract":"The present paper describes the use of indium-tin oxide microelectrodes for manipulating microtubules in solution through interaction with electric fields. The experimental set-up that is described here is a platform for both investigating the fundamental dielectric properties of microtubules and developing methods to exert control over their behaviour in solution. Direct applications of such methods can be found in the development of microsystems integrating nano-machines based on motor proteins such as kinesin or dynein. Electro-rotation of microtubules in solution is presented as an example of interaction between microtubules and electric field","PeriodicalId":170356,"journal":{"name":"2006 International Conference on Microtechnologies in Medicine and Biology","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123212162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
An integrated in situ analyzer for ATP concentration (IISA-ATP) has been developed in this study. Concept of IISA-ATP that has function for cell lysis, extracellular ATP elimination, ATP quantification with luciferin-luciferase reaction and in situ calibration is proposed in this study. A simplified prototype of PDMS based microfluidic chip that has a coiled microchannel pattern for optical detection is developed with micro fabrication technique. As a result of evaluation of the prototype, more than 10-10 M range of ATP was successfully detected
{"title":"Development of in situ ATP quantitative analysis system \"IISA-ATP\"","authors":"N. Fukuzawa, T. Fukuba, T. Fujii","doi":"10.1109/MMB.2006.251544","DOIUrl":"https://doi.org/10.1109/MMB.2006.251544","url":null,"abstract":"An integrated in situ analyzer for ATP concentration (IISA-ATP) has been developed in this study. Concept of IISA-ATP that has function for cell lysis, extracellular ATP elimination, ATP quantification with luciferin-luciferase reaction and in situ calibration is proposed in this study. A simplified prototype of PDMS based microfluidic chip that has a coiled microchannel pattern for optical detection is developed with micro fabrication technique. As a result of evaluation of the prototype, more than 10-10 M range of ATP was successfully detected","PeriodicalId":170356,"journal":{"name":"2006 International Conference on Microtechnologies in Medicine and Biology","volume":"30 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123355196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In our contribution, we present the fabrication of electroporation microchip in detail. The practical experiments of single-cell electroporation with our fabricated microchip will be carried out. Electroporation test efficiency and cell viability tests will be provided. This device enables to reduce the size of samples and thus the use of small amount of reagents. It may also permit to avoid cell separation (transfected cells versus non transfected cells) encountered when traditional bulk electroporation is held
{"title":"A Silicon-Based Single-Cell Electroporation Microchip for Gene Transfer","authors":"Younghak Cho, B. Pioufle, N. Takama, Beomjoon Kim","doi":"10.1109/MMB.2006.251526","DOIUrl":"https://doi.org/10.1109/MMB.2006.251526","url":null,"abstract":"In our contribution, we present the fabrication of electroporation microchip in detail. The practical experiments of single-cell electroporation with our fabricated microchip will be carried out. Electroporation test efficiency and cell viability tests will be provided. This device enables to reduce the size of samples and thus the use of small amount of reagents. It may also permit to avoid cell separation (transfected cells versus non transfected cells) encountered when traditional bulk electroporation is held","PeriodicalId":170356,"journal":{"name":"2006 International Conference on Microtechnologies in Medicine and Biology","volume":"36 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115192384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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