Pub Date : 2015-11-20DOI: 10.4172/2157-7552.1000157
Althani A
Qatar Biobank “QBB” is a large-scale, long term medical research initiative for the population of Qatar, which will serve as a platform and driver of biomedical research to achieve benefits for Qatar’s communities. Following on from the announcement of the Qatar Genome Project in 2013 by Her Highness Sheikha Moza bint Nasser, Chairperson of Qatar Foundation “QF”, a National Genome Committee “NGC” is tasked with the role of driving and advising the development of the Genome Project in Qatar. To ensure the successful implementation and completion of a project of this complexity, it was important to consider carefully the organizational structure of Genome Qatar to enable success, so the decision was to establish QG project within the existing framework of the government of QBB. A road map constitutes of seven key building blocks, were identified to address the critical success factors and be managed and overseen by the Board of Trustee for both QBB and QG program. The seven building blocks are 1-Develop a National Health Information System, 2-Enhancement of the National Biobank, 3-Develop Genomics Infrastructure,4Develop Policy Framework for genomics and precision medicine, 5-Workforce Development ,6Research and Partnership and 7Clinical/Medical implementation. To successfully accomplish this ambitious role a road map is initiated through a pilot phase to establish the infrastructure and human capacity for 12-18 months in order to tackle problems, identify loopholes, analyze the needs and optimize systems that are needed for the entire Qatar Genome project. The aim is to sequence 1000-3000 genomes to develop a good model of practice for regulatory compliant, sample collection and storage, high quality data generation, analysis and annotation pipeline development and data warehouse establishment. Journal of Tissue Science & Engineering J o u r n a l o f T iss ue S cience &ngine e r i n g
{"title":"Qatar Biobank and Qatar Genome Programs Road Map","authors":"Althani A","doi":"10.4172/2157-7552.1000157","DOIUrl":"https://doi.org/10.4172/2157-7552.1000157","url":null,"abstract":"Qatar Biobank “QBB” is a large-scale, long term medical research initiative for the population of Qatar, which will serve as a platform and driver of biomedical research to achieve benefits for Qatar’s communities. Following on from the announcement of the Qatar Genome Project in 2013 by Her Highness Sheikha Moza bint Nasser, Chairperson of Qatar Foundation “QF”, a National Genome Committee “NGC” is tasked with the role of driving and advising the development of the Genome Project in Qatar. To ensure the successful implementation and completion of a project of this complexity, it was important to consider carefully the organizational structure of Genome Qatar to enable success, so the decision was to establish QG project within the existing framework of the government of QBB. A road map constitutes of seven key building blocks, were identified to address the critical success factors and be managed and overseen by the Board of Trustee for both QBB and QG program. The seven building blocks are 1-Develop a National Health Information System, 2-Enhancement of the National Biobank, 3-Develop Genomics Infrastructure,4Develop Policy Framework for genomics and precision medicine, 5-Workforce Development ,6Research and Partnership and 7Clinical/Medical implementation. To successfully accomplish this ambitious role a road map is initiated through a pilot phase to establish the infrastructure and human capacity for 12-18 months in order to tackle problems, identify loopholes, analyze the needs and optimize systems that are needed for the entire Qatar Genome project. The aim is to sequence 1000-3000 genomes to develop a good model of practice for regulatory compliant, sample collection and storage, high quality data generation, analysis and annotation pipeline development and data warehouse establishment. Journal of Tissue Science & Engineering J o u r n a l o f T iss ue S cience &ngine e r i n g","PeriodicalId":17539,"journal":{"name":"Journal of Tissue Science and Engineering","volume":"66 1","pages":"1-3"},"PeriodicalIF":0.0,"publicationDate":"2015-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90899512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-09-24DOI: 10.4172/2157-7552.1000156
L. Frese, B. Sanders, G. Beer, B. Weber, A. Mol, F. Baaijens, S. Hoerstrup
Abstract Introduction: A major challenge associated with heart valve tissue engineering is the in vitro creation of mature tissue structures compliant with native valve functionality. Various cell types have been investigated for heart valve tissue engineering. In addition to prenatal, umbilical cord- and vascular-derived cells, mesenchymal stem cells (MSCs) have gained large interest for tissue engineering purposes, because of their broad differentiation potential. However, bone marrow derived MSCs require a highly invasive harvesting procedure and decline in both cell number and differentiation potential proportionally with the donor’s age. In contrast, adipose derived stem cells (ADSCs) represent an interesting alternative. The ease of repeated access to subcutaneous adipose tissue as well as the less invasive donation procedures provide clear advantages. Therefore, this study investigated the suitability of ADSCs as alternative cell source for tissue engineered heart valves (TEHVs). Methods: Human ADSCs were seeded on TEHV-scaffolds (n=11) made of nonwoven polyglycolic acid coated with poly-4-hydroxybutyrate. TEHVs were cultivated in diastolic-pulse-duplicator-bioreactor systems and subsequently seeded with a superficial layer of ADSC-derived endothelial cells. Quantitative assessment of extracellular matrix composition of the TEHV-leaflets was performed with biochemical analyses for sulphated glycosaminoglycans, hydroxyproline and DNA content. Microstructural evaluation was performed on representative samples of the TEHVleaflets by (immuno-)histochemistry and scanning electron microscopy. The mechanical properties of the ADSC derived TEHV-leaflets were characterized by biaxial tensile tests. Results: ADSC-derived TEHV-leaflets showed a homogenous vital cell distribution throughout the whole leaflet structure that consisted of large amounts of glycosaminoglycans and collagen and was endothelialized. Furthermore, the mechanically stable matrix of the ADSC-derived TEHVs showed a stiffness range in the right order of magnitude for heart valve applications. Conclusion: Human ADSCs represent a promising alternative autologous mesenchymal cell source for TEHVs that is of large clinical relevance due to their easy accessibility, efficient proliferation and excellent tissue formation capacities.
{"title":"Adipose derived tissue engineered heart valve","authors":"L. Frese, B. Sanders, G. Beer, B. Weber, A. Mol, F. Baaijens, S. Hoerstrup","doi":"10.4172/2157-7552.1000156","DOIUrl":"https://doi.org/10.4172/2157-7552.1000156","url":null,"abstract":"Abstract Introduction: A major challenge associated with heart valve tissue engineering is the in vitro creation of mature tissue structures compliant with native valve functionality. Various cell types have been investigated for heart valve tissue engineering. In addition to prenatal, umbilical cord- and vascular-derived cells, mesenchymal stem cells (MSCs) have gained large interest for tissue engineering purposes, because of their broad differentiation potential. However, bone marrow derived MSCs require a highly invasive harvesting procedure and decline in both cell number and differentiation potential proportionally with the donor’s age. In contrast, adipose derived stem cells (ADSCs) represent an interesting alternative. The ease of repeated access to subcutaneous adipose tissue as well as the less invasive donation procedures provide clear advantages. Therefore, this study investigated the suitability of ADSCs as alternative cell source for tissue engineered heart valves (TEHVs). Methods: Human ADSCs were seeded on TEHV-scaffolds (n=11) made of nonwoven polyglycolic acid coated with poly-4-hydroxybutyrate. TEHVs were cultivated in diastolic-pulse-duplicator-bioreactor systems and subsequently seeded with a superficial layer of ADSC-derived endothelial cells. Quantitative assessment of extracellular matrix composition of the TEHV-leaflets was performed with biochemical analyses for sulphated glycosaminoglycans, hydroxyproline and DNA content. Microstructural evaluation was performed on representative samples of the TEHVleaflets by (immuno-)histochemistry and scanning electron microscopy. The mechanical properties of the ADSC derived TEHV-leaflets were characterized by biaxial tensile tests. Results: ADSC-derived TEHV-leaflets showed a homogenous vital cell distribution throughout the whole leaflet structure that consisted of large amounts of glycosaminoglycans and collagen and was endothelialized. Furthermore, the mechanically stable matrix of the ADSC-derived TEHVs showed a stiffness range in the right order of magnitude for heart valve applications. Conclusion: Human ADSCs represent a promising alternative autologous mesenchymal cell source for TEHVs that is of large clinical relevance due to their easy accessibility, efficient proliferation and excellent tissue formation capacities.","PeriodicalId":17539,"journal":{"name":"Journal of Tissue Science and Engineering","volume":"4 1","pages":"1-9"},"PeriodicalIF":0.0,"publicationDate":"2015-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79363247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-09-17DOI: 10.4172/2157-7552.1000154
Demeng Chen, Xinqi Zhong, Kai Wang, Yizhou Jiang
Mesenchymal Stem Cells (MSC) can be obtained from various tissues and differentiate into many different lineages, including osteoblasts, adipocytes, chondrocytes, cardiomyocytes, hepatocytes and neural cells both in vivo and in vitro. However, the ability of MSC to differentiate into specific lineages seems to be restricted and requires a deeper understanding of the genetic and epigenetic mechanisms. Epigenetic mechanism refers to a process that regulates heritable alterations in gene expression without changing the DNA sequence. SWI/SNF (SWItch/Sucrose Non-Fermentable), a chromatin-remodeling complex serves as an ideal intervention point for lineage manipulation of MSC. In this review, we discuss the importance of SWI/SNF chromatin remodeling complex in regulating the fate determination of MSC. We propose that selectively manipulation of subunits of SWI/SNF will enhance the lineagespecific differentiation of MSC and improve therapeutic application of MSC.
{"title":"SWI/SNF Chromatin Remodeling Complex in Regulating Mesenchymal Stem Cell Lineage Specification","authors":"Demeng Chen, Xinqi Zhong, Kai Wang, Yizhou Jiang","doi":"10.4172/2157-7552.1000154","DOIUrl":"https://doi.org/10.4172/2157-7552.1000154","url":null,"abstract":"Mesenchymal Stem Cells (MSC) can be obtained from various tissues and differentiate into many different lineages, including osteoblasts, adipocytes, chondrocytes, cardiomyocytes, hepatocytes and neural cells both in vivo and in vitro. However, the ability of MSC to differentiate into specific lineages seems to be restricted and requires a deeper understanding of the genetic and epigenetic mechanisms. Epigenetic mechanism refers to a process that regulates heritable alterations in gene expression without changing the DNA sequence. SWI/SNF (SWItch/Sucrose Non-Fermentable), a chromatin-remodeling complex serves as an ideal intervention point for lineage manipulation of MSC. In this review, we discuss the importance of SWI/SNF chromatin remodeling complex in regulating the fate determination of MSC. We propose that selectively manipulation of subunits of SWI/SNF will enhance the lineagespecific differentiation of MSC and improve therapeutic application of MSC.","PeriodicalId":17539,"journal":{"name":"Journal of Tissue Science and Engineering","volume":"174 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2015-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83538469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-09-17DOI: 10.4172/2157-7552.1000155
L. Pighinelli, M. Guimarães, Paz Rl
Nature itself uses materials like cellulose to provide the structure of plants, chitin as the exoskeleton of several insects and molluscs, collagen for mechanical support in connective tissues and so on. At present, the socioeconomic situation of the modern world has raised the interest in renewable materials to use in regenerative medicine. The composition of Chitosan and/or Calcium Phosphates are derived from the junction of two or more different materials, containing organic and inorganic materials, including characteristics of both materials like bioactivity and biodegradability and biocompatibility with human tissues. The chemical characteristics of chitosan and nano B-TCP / HAp complex showed that both of the components organic and inorganic exist in the material showing a good stability of the nano-ceramic formation in the chitosan salt solutions. All complex by Zeta-Potential, showing also a new method of preparations nanoparticles of calcium phosphates in chitosan solution from commercial calcium phosphates in micro size. These materials can be used in future for medical applications as a base for scaffolds production and as implants in regenerative medicine. Journal of Tissue Science & Engineering J o u r n a l o f T iss ue S cience &ngine e r i n g
{"title":"Properties of hydrochloric chitosan solutions modified with nano-calcium phosphate complex","authors":"L. Pighinelli, M. Guimarães, Paz Rl","doi":"10.4172/2157-7552.1000155","DOIUrl":"https://doi.org/10.4172/2157-7552.1000155","url":null,"abstract":"Nature itself uses materials like cellulose to provide the structure of plants, chitin as the exoskeleton of several insects and molluscs, collagen for mechanical support in connective tissues and so on. At present, the socioeconomic situation of the modern world has raised the interest in renewable materials to use in regenerative medicine. The composition of Chitosan and/or Calcium Phosphates are derived from the junction of two or more different materials, containing organic and inorganic materials, including characteristics of both materials like bioactivity and biodegradability and biocompatibility with human tissues. The chemical characteristics of chitosan and nano B-TCP / HAp complex showed that both of the components organic and inorganic exist in the material showing a good stability of the nano-ceramic formation in the chitosan salt solutions. All complex by Zeta-Potential, showing also a new method of preparations nanoparticles of calcium phosphates in chitosan solution from commercial calcium phosphates in micro size. These materials can be used in future for medical applications as a base for scaffolds production and as implants in regenerative medicine. Journal of Tissue Science & Engineering J o u r n a l o f T iss ue S cience &ngine e r i n g","PeriodicalId":17539,"journal":{"name":"Journal of Tissue Science and Engineering","volume":"12 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2015-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74852450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-08-31DOI: 10.4172/2157-7552.1000153
P. Ghosh, David Oehme, T. Goldschlager, Susan Shimon, Jiehua Wu, S. Stuckey, Mark Williamson, J. Rosenfeld, G. Jenkin
Circumferential tears of the Annulus Fibrosus (AF) are frequently observed pathological features of degenerate lumbar discs and have been associated with vascular propagation and the generation of low back pain. In order to evaluate the potential of novel biological agents to repair annular defects and arrest disc degeneration we required an animal model that would permit injection of cells or their cryoprotectant into adjacent lumbar discs of the same animal. Three lumbar discs (L2/3, L3/4 and L4/5) of 6 sheep were subjected to a peripheral lateral annular surgical incision. The adjacent uninjured lumbar L1/2 and L5/6 discs served as uninjured controls. After three months the spines were radiographed and disc height indices (DHI) calculated and Pfirrmann disc degeneration scores determined from MRI spinal images. Isolated lumbar discs were analysed morphologically, histologically and biochemically using published procedures. Disc height index measurements of injured discs revealed an average decrease of 23.67% relative to baseline values (p<0.0001). The corresponding MRI Pfirrmann degeneration scores were significantly higher than non-injured control discs (p<0.05), as were their morphology scores (p<0.005). The sulphated - glycosaminoglycan content, of the Nucleus Pulposus (NP) and injured side of the AF of lumbar discs, were significantly lower than control discs (p<0.05 and p<0.0005) respectively. Conversely, the DNA levels of the injured side of the AF were higher than the uninjured side (p<0.05). The histological scores showed higher degenerative changes in injured than in control discs (p<0.005). For all parameters monitored in this study no statistical differences were observed between the three injured lumbar discs confirming their uniform response to injury. This study therefore confirmed the suitability of this large animal model for evaluating the potential of biologicals to reconstitute degenerate ovine lumbar discs relative to their carriers/cryoprotectant.
{"title":"Radiological, Morphological, Histological and Biochemical Changes of Lumbar Discs in an Animal Model of Disc Degeneration Suitable for Evaluating the Potential Regenerative Capacity of Novel Biological Agents.","authors":"P. Ghosh, David Oehme, T. Goldschlager, Susan Shimon, Jiehua Wu, S. Stuckey, Mark Williamson, J. Rosenfeld, G. Jenkin","doi":"10.4172/2157-7552.1000153","DOIUrl":"https://doi.org/10.4172/2157-7552.1000153","url":null,"abstract":"Circumferential tears of the Annulus Fibrosus (AF) are frequently observed pathological features of degenerate lumbar discs and have been associated with vascular propagation and the generation of low back pain. In order to evaluate the potential of novel biological agents to repair annular defects and arrest disc degeneration we required an animal model that would permit injection of cells or their cryoprotectant into adjacent lumbar discs of the same animal. Three lumbar discs (L2/3, L3/4 and L4/5) of 6 sheep were subjected to a peripheral lateral annular surgical incision. The adjacent uninjured lumbar L1/2 and L5/6 discs served as uninjured controls. After three months the spines were radiographed and disc height indices (DHI) calculated and Pfirrmann disc degeneration scores determined from MRI spinal images. Isolated lumbar discs were analysed morphologically, histologically and biochemically using published procedures. Disc height index measurements of injured discs revealed an average decrease of 23.67% relative to baseline values (p<0.0001). The corresponding MRI Pfirrmann degeneration scores were significantly higher than non-injured control discs (p<0.05), as were their morphology scores (p<0.005). The sulphated - glycosaminoglycan content, of the Nucleus Pulposus (NP) and injured side of the AF of lumbar discs, were significantly lower than control discs (p<0.05 and p<0.0005) respectively. Conversely, the DNA levels of the injured side of the AF were higher than the uninjured side (p<0.05). The histological scores showed higher degenerative changes in injured than in control discs (p<0.005). For all parameters monitored in this study no statistical differences were observed between the three injured lumbar discs confirming their uniform response to injury. This study therefore confirmed the suitability of this large animal model for evaluating the potential of biologicals to reconstitute degenerate ovine lumbar discs relative to their carriers/cryoprotectant.","PeriodicalId":17539,"journal":{"name":"Journal of Tissue Science and Engineering","volume":"24 1","pages":"1000153"},"PeriodicalIF":0.0,"publicationDate":"2015-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81542379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-08-26DOI: 10.4172/2157-7552.S1.022
Victor Lopez Davila
Methods: AZD6244-containing GCPQ micelles and DOPE/DC-cholesterol liposomes were fabricated using sonication and extrusion methods respectively. These formulations were tested together with the free drug in HCT116 cell monolayers and a collagen-based 3D cancer model to determine differences in efficacy between both models. Fluorescent nanoparticles were used to determine their ability to penetrate the scaffold and reach its core.
方法:采用超声法制备含GCPQ胶束的azd6244，采用挤压法制备含DOPE/ dc -胆固醇脂质体。这些配方与游离药物一起在HCT116细胞单层和基于胶原蛋白的3D癌症模型中进行测试，以确定两种模型之间的疗效差异。荧光纳米颗粒被用来测定它们穿透支架并到达其核心的能力。
{"title":"Efficacy of therapeutic nanoformulations in a collagen-based three-dimensional colorectal cancer in vitro model","authors":"Victor Lopez Davila","doi":"10.4172/2157-7552.S1.022","DOIUrl":"https://doi.org/10.4172/2157-7552.S1.022","url":null,"abstract":"Methods: AZD6244-containing GCPQ micelles and DOPE/DC-cholesterol liposomes were fabricated using sonication and extrusion methods respectively. These formulations were tested together with the free drug in HCT116 cell monolayers and a collagen-based 3D cancer model to determine differences in efficacy between both models. Fluorescent nanoparticles were used to determine their ability to penetrate the scaffold and reach its core.","PeriodicalId":17539,"journal":{"name":"Journal of Tissue Science and Engineering","volume":"73 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85746906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-08-26DOI: 10.4172/2157-7552.S1.020
Gordon Blunn
{"title":"Augmenting the fixation of orthopaedic implants with stem cells","authors":"Gordon Blunn","doi":"10.4172/2157-7552.S1.020","DOIUrl":"https://doi.org/10.4172/2157-7552.S1.020","url":null,"abstract":"","PeriodicalId":17539,"journal":{"name":"Journal of Tissue Science and Engineering","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75688776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-08-14DOI: 10.4172/2157-7552.1000152
C. KarlaL.Tovar, Genaro Tamayo, Alej, Roana Donohue, Takaomi Kobayashi, A. RosaA.Saucedo
Polyvinyl Alcohol (PVA) and Hydroxy Ethyl Cellulose (HEC) were used to prepare hydrogel for tissue regeneration. Female rabbits were used to evaluate the obtained hydrogels for the regeneration of adipose tissue. Mechanical and biocompatible properties were evaluated. AFM (Atomic Force Microcopy) and SEM (Scanning Electron Microscopy) showed the roughness around 5.447 nm and pore size from 1 to 7.9 μm. In vivo tests were conducted during two years in female rabbits. Histological images showed stable fat formation over long-term without adverse reaction or necrosis. The obtained results indicated that PHEC30 hydrogel may provide a viable approach for the regeneration of adipose tissue in female rabbits as first step as an alternative solution for women who suffered a radical mastectomy.
{"title":"Obtaining of Hydrogels using PVA and HEC for Adipose Tissue Regeneration","authors":"C. KarlaL.Tovar, Genaro Tamayo, Alej, Roana Donohue, Takaomi Kobayashi, A. RosaA.Saucedo","doi":"10.4172/2157-7552.1000152","DOIUrl":"https://doi.org/10.4172/2157-7552.1000152","url":null,"abstract":"Polyvinyl Alcohol (PVA) and Hydroxy Ethyl Cellulose (HEC) were used to prepare hydrogel for tissue regeneration. Female rabbits were used to evaluate the obtained hydrogels for the regeneration of adipose tissue. Mechanical and biocompatible properties were evaluated. AFM (Atomic Force Microcopy) and SEM (Scanning Electron Microscopy) showed the roughness around 5.447 nm and pore size from 1 to 7.9 μm. In vivo tests were conducted during two years in female rabbits. Histological images showed stable fat formation over long-term without adverse reaction or necrosis. The obtained results indicated that PHEC30 hydrogel may provide a viable approach for the regeneration of adipose tissue in female rabbits as first step as an alternative solution for women who suffered a radical mastectomy.","PeriodicalId":17539,"journal":{"name":"Journal of Tissue Science and Engineering","volume":"15 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2015-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89017356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-08-10DOI: 10.4172/2157-7552.1000E130
Shannon L. Speer, Gretchen E Schreyack, G. Bowlin
A renewed interest in medical-grade honey is revitalizing the clinical and biomedical engineering communities. The primary driving force for this revitalization has been the goal of developing non-antibiotic alternatives and effective new ways to prevent and fight bacterial infections. This application of honey is not unexpected or new since the use of medicinal honey simply fell out of fashion when antibiotics revolutionized medicine over one hundred years ago. Prior to antibiotics, and dating back as far as 600 B.C., honey had been extensively used in the treatment of infections and burns [1]. More importantly, the broad antimicrobial spectrum of honey makes it a great and powerful alternative to antibiotics when dealing with wound infections. However, care must be used in selection because the antimicrobial spectrum of the honey is very much dependent on the type (source of nectar) of honey used [2]. For this reason, a specific honey, Manuka honey, appears to be leading the charge in transforming the medical industry due to its unique composition and derived functionality. Its functionality includes a broadband anti-bacterial, anti-inflammatory, and enhanced wound healing capacity that may be the tissue engineering trifecta and deserves further consideration as an essential tissue engineering ingredient (i.e. incorporation into templates).
{"title":"Manuka Honey: A Tissue Engineering Essential Ingredient","authors":"Shannon L. Speer, Gretchen E Schreyack, G. Bowlin","doi":"10.4172/2157-7552.1000E130","DOIUrl":"https://doi.org/10.4172/2157-7552.1000E130","url":null,"abstract":"A renewed interest in medical-grade honey is revitalizing the clinical and biomedical engineering communities. The primary driving force for this revitalization has been the goal of developing non-antibiotic alternatives and effective new ways to prevent and fight bacterial infections. This application of honey is not unexpected or new since the use of medicinal honey simply fell out of fashion when antibiotics revolutionized medicine over one hundred years ago. Prior to antibiotics, and dating back as far as 600 B.C., honey had been extensively used in the treatment of infections and burns [1]. More importantly, the broad antimicrobial spectrum of honey makes it a great and powerful alternative to antibiotics when dealing with wound infections. However, care must be used in selection because the antimicrobial spectrum of the honey is very much dependent on the type (source of nectar) of honey used [2]. For this reason, a specific honey, Manuka honey, appears to be leading the charge in transforming the medical industry due to its unique composition and derived functionality. Its functionality includes a broadband anti-bacterial, anti-inflammatory, and enhanced wound healing capacity that may be the tissue engineering trifecta and deserves further consideration as an essential tissue engineering ingredient (i.e. incorporation into templates).","PeriodicalId":17539,"journal":{"name":"Journal of Tissue Science and Engineering","volume":"10 1","pages":"1-3"},"PeriodicalIF":0.0,"publicationDate":"2015-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83534925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-08-01DOI: 10.4172/2157-7552.S1.021
Roberto Ebensperger
Background:Numerous investigations suggest that Mesenchymal Stem Cells (MSCs) in general represent a valuable tool for therapy of symptoms related to chronic inflammatory diseases. Blue Horizon Stem Cell Therapy Program is a leading provider of adult and children’s stem cell therapies. Uniquely we have safely and efficiently treated more 600 patients with documenting each procedure. Materials and methods: The purpose of our study is primarily to monitor the immune response in order to validate the safety of intravenous infusion of human umbilical cord blood derived MSCs (UC-MSCs), and secondly, to evaluate effects on biomarkers associated with chronic inflammation. Nine patients were treated for conditions associated with chronic inflammation and for the purpose of anti-aging. They have been given one intravenous infusion of UC-MSCs. Results: Our study of blood test markers of 9 patients with chronic inflammation before and within three months after MSCs treatment demonstrates that there is no significant changes and MSCs treatment was safe for the patients. Analysis of different indicators of chronic inflammation and aging included in initial, 24hours, two weeks and three months protocols showed that stem cell treatment was safe for the patients; there were no adverse reactions. Moreover data from follow up protocols demonstrates significant improvement in energy level, hair, nails growth and skin conditions. Conclusions: Intravenously administered UCMSCs were safe and effective in the improvement of symptoms related to chronic inflammation. Further close monitoring and inclusion of more patients are necessary to fully characterize the advantages of UC-MSCs application in treatment of symptoms related to chronic inflammation.
{"title":"Nanoencapsulation of stem cells and regenerative medicine","authors":"Roberto Ebensperger","doi":"10.4172/2157-7552.S1.021","DOIUrl":"https://doi.org/10.4172/2157-7552.S1.021","url":null,"abstract":"Background:Numerous investigations suggest that Mesenchymal Stem Cells (MSCs) in general represent a valuable tool for therapy of symptoms related to chronic inflammatory diseases. Blue Horizon Stem Cell Therapy Program is a leading provider of adult and children’s stem cell therapies. Uniquely we have safely and efficiently treated more 600 patients with documenting each procedure. Materials and methods: The purpose of our study is primarily to monitor the immune response in order to validate the safety of intravenous infusion of human umbilical cord blood derived MSCs (UC-MSCs), and secondly, to evaluate effects on biomarkers associated with chronic inflammation. Nine patients were treated for conditions associated with chronic inflammation and for the purpose of anti-aging. They have been given one intravenous infusion of UC-MSCs. Results: Our study of blood test markers of 9 patients with chronic inflammation before and within three months after MSCs treatment demonstrates that there is no significant changes and MSCs treatment was safe for the patients. Analysis of different indicators of chronic inflammation and aging included in initial, 24hours, two weeks and three months protocols showed that stem cell treatment was safe for the patients; there were no adverse reactions. Moreover data from follow up protocols demonstrates significant improvement in energy level, hair, nails growth and skin conditions. Conclusions: Intravenously administered UCMSCs were safe and effective in the improvement of symptoms related to chronic inflammation. Further close monitoring and inclusion of more patients are necessary to fully characterize the advantages of UC-MSCs application in treatment of symptoms related to chronic inflammation.","PeriodicalId":17539,"journal":{"name":"Journal of Tissue Science and Engineering","volume":"os-55 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87094238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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