Pub Date : 2025-02-01DOI: 10.1016/j.lssr.2024.10.011
Peter Wostyn , Piet Goddaer
Spaceflight occurs under extreme environmental conditions that pose significant risks to the physical and mental health and well-being of astronauts. Certain factors, such as prolonged isolation, monotony, disrupted circadian rhythms, heavy workload, and weightlessness in space, can trigger psychological distress and may contribute to a variety of mental health problems, including mood and anxiety disturbances. Recent findings regarding spaceflight-associated alterations in cerebrospinal fluid spaces, demonstrating enlargement of the brain's perivascular spaces from preflight to postflight, at least suggest reduced glymphatic clearance in microgravity, and have raised concerns about long-term cognitive health in astronauts. Therefore, it is critical for future long-duration human exploration missions to identify, develop and validate all potentially effective long-term countermeasures capable of reducing the risk of perivascular space enlargement and impaired glymphatic transport in space mission crews. Furthermore, it is crucial to implement effective strategies that would allow crew members to maintain optimal psychological well-being during future long-duration space exploration. In the present paper, we propose “immersive gamma music” as an add-on countermeasure that in combination with existing countermeasures can optimize glymphatic clearance in astronauts while improving their mental well-being. If confirmed, this approach could enrich the practice of space medicine, and might become increasingly important, given the plans for future human missions, including a return to the Moon and manned missions to Mars.
{"title":"Immersive gamma music as a tool for enhancing glymphatic clearance in astronauts while improving their mental well-being","authors":"Peter Wostyn , Piet Goddaer","doi":"10.1016/j.lssr.2024.10.011","DOIUrl":"10.1016/j.lssr.2024.10.011","url":null,"abstract":"<div><div>Spaceflight occurs under extreme environmental conditions that pose significant risks to the physical and mental health and well-being of astronauts. Certain factors, such as prolonged isolation, monotony, disrupted circadian rhythms, heavy workload, and weightlessness in space, can trigger psychological distress and may contribute to a variety of mental health problems, including mood and anxiety disturbances. Recent findings regarding spaceflight-associated alterations in cerebrospinal fluid spaces, demonstrating enlargement of the brain's perivascular spaces from preflight to postflight, at least suggest reduced glymphatic clearance in microgravity, and have raised concerns about long-term cognitive health in astronauts. Therefore, it is critical for future long-duration human exploration missions to identify, develop and validate all potentially effective long-term countermeasures capable of reducing the risk of perivascular space enlargement and impaired glymphatic transport in space mission crews. Furthermore, it is crucial to implement effective strategies that would allow crew members to maintain optimal psychological well-being during future long-duration space exploration. In the present paper, we propose “immersive gamma music” as an add-on countermeasure that in combination with existing countermeasures can optimize glymphatic clearance in astronauts while improving their mental well-being. If confirmed, this approach could enrich the practice of space medicine, and might become increasingly important, given the plans for future human missions, including a return to the Moon and manned missions to Mars.</div></div>","PeriodicalId":18029,"journal":{"name":"Life Sciences in Space Research","volume":"44 ","pages":"Pages 86-89"},"PeriodicalIF":2.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143047172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-01DOI: 10.1016/j.lssr.2024.12.004
Rahul Kumar , Ethan Waisberg , Joshua Ong , Karsten Chima , Dylan Amiri , Alireza Tavakkoli
Spaceflight-Associated Neuro-Ocular Syndrome (SANS) presents a critical risk in long-duration missions, with microgravity-induced changes that threaten astronaut vision and mission outcomes. Current SANS monitoring, limited to pre- and post-flight exams, lacks in-flight diagnostics, highlighting an urgent need for autonomous tools capable of real-time assessment. Grok, an AI platform by xAI, offers promising potential as an advanced diagnostic tool for space-based health monitoring. Originally developed for broader applications, Grok's high-resolution imaging capabilities could be adapted to detect early SANS indicators such as optic nerve edema and shifts in globe morphology, changes linked to fluid redistribution in space. However, realizing this vision requires algorithmic and hardware adjustments to address the unique physiological shifts astronauts experience. By advancing Grok's diagnostic capability, we strongly believe astronauts could manage SANS autonomously, bringing much-needed real-time, high-accuracy diagnostics to isolated, high-stakes environments—essential as humanity embarks on increasingly ambitious missions to Mars and beyond
{"title":"Optimizing autonomous artificial intelligence diagnostics for neuro-ocular health in space missions","authors":"Rahul Kumar , Ethan Waisberg , Joshua Ong , Karsten Chima , Dylan Amiri , Alireza Tavakkoli","doi":"10.1016/j.lssr.2024.12.004","DOIUrl":"10.1016/j.lssr.2024.12.004","url":null,"abstract":"<div><div>Spaceflight-Associated Neuro-Ocular Syndrome (SANS) presents a critical risk in long-duration missions, with microgravity-induced changes that threaten astronaut vision and mission outcomes. Current SANS monitoring, limited to pre- and post-flight exams, lacks in-flight diagnostics, highlighting an urgent need for autonomous tools capable of real-time assessment. Grok, an AI platform by xAI, offers promising potential as an advanced diagnostic tool for space-based health monitoring. Originally developed for broader applications, Grok's high-resolution imaging capabilities could be adapted to detect early SANS indicators such as optic nerve edema and shifts in globe morphology, changes linked to fluid redistribution in space. However, realizing this vision requires algorithmic and hardware adjustments to address the unique physiological shifts astronauts experience. By advancing Grok's diagnostic capability, we strongly believe astronauts could manage SANS autonomously, bringing much-needed real-time, high-accuracy diagnostics to isolated, high-stakes environments—essential as humanity embarks on increasingly ambitious missions to Mars and beyond</div></div>","PeriodicalId":18029,"journal":{"name":"Life Sciences in Space Research","volume":"44 ","pages":"Pages 64-66"},"PeriodicalIF":2.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143047197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-01DOI: 10.1016/j.lssr.2024.08.005
Viktor S. Kokhan , Kirill Chaprov , Denis A. Abaimov , Maxim S. Nesterov , Vladimir A. Pikalov
Exposure to ionizing radiation during manned deep space missions to Mars could lead to functional impairments of the central nervous system, which may compromise the success of the mission and affect the quality of life for returning astronauts. Along with radiation-induced changes in cognitive abilities and emotional status, the effects of increased motor activity were observed. The mechanisms behind these phenomena still remain unresolved. We conducted a study on grip strength, locomotor activity and intrasession habituation to novelty in 5-month-old rats after exposure to radiation (combined 0.4 Gy gamma-rays and 0.14 Gy 12C nuclei). At the same time, we carried out neurochemical and molecular analysis of the nucleus accumbens (NAc) and the dorsal striatum (dST). The study revealed radiation-induced hyperlocomotion and enhanced habituation. It also showed an increase in choline concentration and a decreased in 5-hydroxyindoleacetic acid concentration in the NAc after irradiation. In addition to this, a down-regulation of syntaxin 1A in NAc and dST as well as up-regulation α-synuclein in NAc were observed. The obtained data indicate both the damaging effect of irradiation on striatum tissues and the initiation of neuronal/axonal regeneration processes. It is hypothesized that the increase in choline concentration in NAc and the decreased content of syntaxin 1A in dST may be the part of the mechanism responsible for the radiation-induced hyperlocomotion.
{"title":"Combined irradiation by gamma-rays and carbon-12 nuclei caused hyperlocomotion and change in striatal metabolism of rats","authors":"Viktor S. Kokhan , Kirill Chaprov , Denis A. Abaimov , Maxim S. Nesterov , Vladimir A. Pikalov","doi":"10.1016/j.lssr.2024.08.005","DOIUrl":"10.1016/j.lssr.2024.08.005","url":null,"abstract":"<div><div>Exposure to ionizing radiation during manned deep space missions to Mars could lead to functional impairments of the central nervous system, which may compromise the success of the mission and affect the quality of life for returning astronauts. Along with radiation-induced changes in cognitive abilities and emotional status, the effects of increased motor activity were observed. The mechanisms behind these phenomena still remain unresolved. We conducted a study on grip strength, locomotor activity and intrasession habituation to novelty in 5-month-old rats after exposure to radiation (combined 0.4 Gy gamma-rays and 0.14 Gy <sup>12</sup>C nuclei). At the same time, we carried out neurochemical and molecular analysis of the nucleus accumbens (NAc) and the dorsal striatum (dST). The study revealed radiation-induced hyperlocomotion and enhanced habituation. It also showed an increase in choline concentration and a decreased in 5-hydroxyindoleacetic acid concentration in the NAc after irradiation. In addition to this, a down-regulation of syntaxin 1A in NAc and dST as well as up-regulation α-synuclein in NAc were observed. The obtained data indicate both the damaging effect of irradiation on striatum tissues and the initiation of neuronal/axonal regeneration processes. It is hypothesized that the increase in choline concentration in NAc and the decreased content of syntaxin 1A in dST may be the part of the mechanism responsible for the radiation-induced hyperlocomotion.</div></div>","PeriodicalId":18029,"journal":{"name":"Life Sciences in Space Research","volume":"44 ","pages":"Pages 99-107"},"PeriodicalIF":2.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142212532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The paper presents the variations of space radiation (primary and secondary galactic cosmic rays (GCR) absorbed dose rate in silicon and flux) measured during the first-ever commercial suborbital flight of the Virgin Galactic (VG) SpaceShipTwo Unity on 29 June 2023. A Portable Dosimeter-Spectrometer Liulin-CNR-VG is used. It is developed in the Space Research and Technology Institute, Bulgarian Academy of Sciences (SRTI-BAS) under a scientific contract with National Research Council of Italy (CNR), Italy. Liulin-CNR-VG size is 63х54 × 23 mm. Its weight is 0.092 kg. During the first part of the SpaceShipTwo flight, up to 14.4 km, the dose rate rises from 0.058 μGy h-1 up to 2.5 μGy h-1. Above the altitude of 30 km, the dose rate falls to 2.2 μGy h-1, while the dose to flux ratio increases to values about 1.0 nGy cm2 particle-1. The latter confirms the outcomes of previous balloon experiments, i.e. the change of the composition of the radiation field of the GCR and secondary radiation source from predominantly light particles as electrons, pions and muons towards heavier particles as protons and neutrons. On the descending part of the flight, one maximum in the flux and dose rate curves is obtained as Regener-Pfotzer maximum (R-PM). The flux calculated by the moving avervage is equal to 1.2 cm-2 s-1 and the dose rate is equal to 2.9 μGy h-1 at an altitude of 13 km. These values are well in line with those expected in conditions of relatively high solar activity, such as during the flight. The dose rates measured by Liulin-CNR-VG are in good agreement with other Liulin data, such as those recorded during balloon flights in 2005 and 2015 and civil aviation flights. The calculated total equivalent dose rate during the VG SpaceShipTwo flight is 7.46 μSv for 1.22 h. This reveals that there is a very small radiation risk for the pilots and astronauts flying at the VG SpaceShipTwo up to 85.1 1 km altitude.
{"title":"Space radiation measured during first-ever commercial suborbital mission on Virgin Galactic SpaceShipTwo Unity on 29 June 2023","authors":"Tsvetan Dachev , Pantaleone Carlucci , Francesco Cairo , Borislav Tomov , Yuri Matviichuk , Plamen Dimitrov , Mityo Mitev , Malina Jordanova , Lucia Paciucci","doi":"10.1016/j.lssr.2024.09.003","DOIUrl":"10.1016/j.lssr.2024.09.003","url":null,"abstract":"<div><div>The paper presents the variations of space radiation (primary and secondary galactic cosmic rays (GCR) absorbed dose rate in silicon and flux) measured during the first-ever commercial suborbital flight of the Virgin Galactic (VG) SpaceShipTwo Unity on 29 June 2023. A Portable Dosimeter-Spectrometer Liulin-CNR-VG is used. It is developed in the Space Research and Technology Institute, Bulgarian Academy of Sciences (SRTI-BAS) under a scientific contract with National Research Council of Italy (CNR), Italy. Liulin-CNR-VG size is 63х54 × 23 mm. Its weight is 0.092 kg. During the first part of the SpaceShipTwo flight, up to 14.4 km, the dose rate rises from 0.058 μGy h<sup>-1</sup> up to 2.5 μGy h<sup>-1</sup>. Above the altitude of 30 km, the dose rate falls to 2.2 μGy h<sup>-1</sup>, while the dose to flux ratio increases to values about 1.0 nGy cm<sup>2</sup> particle<sup>-1</sup>. The latter confirms the outcomes of previous balloon experiments, i.e. the change of the composition of the radiation field of the GCR and secondary radiation source from predominantly light particles as electrons, pions and muons towards heavier particles as protons and neutrons. On the descending part of the flight, one maximum in the flux and dose rate curves is obtained as Regener-Pfotzer maximum (R-PM). The flux calculated by the moving avervage is equal to 1.2 cm<sup>-2</sup> s<sup>-1</sup> and the dose rate is equal to 2.9 μGy h<sup>-1</sup> at an altitude of 13 km. These values are well in line with those expected in conditions of relatively high solar activity, such as during the flight. The dose rates measured by Liulin-CNR-VG are in good agreement with other Liulin data, such as those recorded during balloon flights in 2005 and 2015 and civil aviation flights. The calculated total equivalent dose rate during the VG SpaceShipTwo flight is 7.46 μSv for 1.22 h. This reveals that there is a very small radiation risk for the pilots and astronauts flying at the VG SpaceShipTwo up to 85.1 1 km altitude.</div></div>","PeriodicalId":18029,"journal":{"name":"Life Sciences in Space Research","volume":"44 ","pages":"Pages 126-133"},"PeriodicalIF":2.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143047204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-01DOI: 10.1016/j.lssr.2024.12.002
Frieda B. Taub, Kate M. McGrath-Flinn, Natalie E. Stillwell, Rachel Haden Kasbohm
We expect to develop self-sustaining extraterrestrial colonies, and they will approach being closed ecological systems. Using simple closed ecosystems containing Daphnia magna, three species of algae, and microbes, we tested multiple conditions to study long-term organism survival, which is only possible with adequate nutrient recycling. Closed and open systems behaved differently from one another at high nitrate concentrations; in closed systems, the animals were dead by day 14; in open systems, the Daphnia populations persisted beyond 273 days. Daphnia deaths were associated with increased pH and O2 caused by greater algal photosynthesis and the lack of exchange with the atmosphere. Replicate variability that used small Daphnia suggested that inadequate grazing capability allowed algae to create conditions unfavorable to Daphnia survival. Over months, algal and Daphnia abundance decreased, presumably because of inadequate nutrient recycling; these populations increased temporarily after the addition of nutrients. The addition of natural lake organisms did not increase the nutrient-recycling capabilities of the systems. Understanding the mechanisms of closed systems will be useful in implementing biological processes in managing life support systems.
{"title":"Behavior of simple closed ecological systems; lower nutrient concentrations allow longer persistence of grazer populations","authors":"Frieda B. Taub, Kate M. McGrath-Flinn, Natalie E. Stillwell, Rachel Haden Kasbohm","doi":"10.1016/j.lssr.2024.12.002","DOIUrl":"10.1016/j.lssr.2024.12.002","url":null,"abstract":"<div><div>We expect to develop self-sustaining extraterrestrial colonies, and they will approach being closed ecological systems. Using simple closed ecosystems containing <em>Daphnia magna</em>, three species of algae, and microbes, we tested multiple conditions to study long-term organism survival, which is only possible with adequate nutrient recycling. Closed and open systems behaved differently from one another at high nitrate concentrations; in closed systems, the animals were dead by day 14; in open systems, the <em>Daphnia</em> populations persisted beyond 273 days. <em>Daphnia</em> deaths were associated with increased pH and O<sub>2</sub> caused by greater algal photosynthesis and the lack of exchange with the atmosphere. Replicate variability that used small <em>Daphnia</em> suggested that inadequate grazing capability allowed algae to create conditions unfavorable to <em>Daphnia</em> survival. Over months, algal and <em>Daphnia</em> abundance decreased, presumably because of inadequate nutrient recycling; these populations increased temporarily after the addition of nutrients. The addition of natural lake organisms did not increase the nutrient-recycling capabilities of the systems. Understanding the mechanisms of closed systems will be useful in implementing biological processes in managing life support systems.</div></div>","PeriodicalId":18029,"journal":{"name":"Life Sciences in Space Research","volume":"44 ","pages":"Pages 47-57"},"PeriodicalIF":2.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143047165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-01DOI: 10.1016/j.lssr.2024.11.005
Hong-Yun Nie , Jun Ge , Kai-Ge Liu , Yuan Yue , Hao Li , Hai-Guan Lin , Tao Zhang , Hong-Feng Yan , Bing-Xin Xu , Hong-Wei Sun , Jian-Wu Yang , Shao-Yan Si , Jin-Lian Zhou , Yan Cui
<div><h3>Background</h3><div>Currently, there is limited research on the impact of abdominal infection on intestinal damage under microgravity conditions. Cordyceps polysaccharide (CPS), the main active ingredient of Cordyceps, has demonstrated various pharmacological effects, including anti-inflammatory, antioxidant, and immunomodulatory properties. Moxifloxacin (MXF) is a fourth-generation quinolone antibiotic that is believed to have a dual regulatory effect on immune system activation and suppression. Our objective was to investigate the effects of MXF plus CPS on the intestinal barrier damage due to abdominal infection under microgravity.</div></div><div><h3>Methods</h3><div>The hindlimb unloading model in rats was employed to simulate microgravity. The rat model of abdominal infection was established by cecal ligation and puncture (CLP). MXF, CPS and the combination of the two drugs were used to treat CLP-rats in simulated microgravity. We assessed histopathological changes of ileum by hematoxylin and eosin staining. The intestinal ultrastructure was observed under transmission electron microscopy. Additionally, the expression of intestinal barrier proteins RegIII α/γ and MUC2 was detected by Western blot analysis, while the localization of these proteins within the ileum was examined using immunohistochemistry. Cytometric bead array (CBA) was employed to detect cytokine including IL-6, TNF-α, IL-1β, IL-1α, CXCL-1, MCP-1, IL-17A, IL-18, and IL-33. Flow cytometry analysis was conducted to determine the percentages of Treg cells, M1 macrophages, M2 macrophages, T cells and CD8<sup>+</sup><em>T</em> cells.</div></div><div><h3>Results</h3><div>The results showed that compared with the normal gravity groups, the simulated microgravity groups exhibited a significant decrease in RegIII α/γ protein expression, an increase in M1 macrophage frequency, and elevated levels of TNF-α, IL-1α, MCP-1 and IL-6. Notably, the combined application of MXF and CPS effectively mitigated intestinal barrier damage in CLP-rats exposed to microgravity, as evidenced by alleviated ultrastructural and pathological impairments in ileum, along with increased expression of key intestinal barrier proteins MUC2 and RegIII α/γ. Furthermore, the combination therapy enhances the proportion of T cells, CD8<sup>+</sup> <em>T</em> cells, and M2 macrophages in septic rats exposed to simulated microgravity while reducing the frequency of Treg cells and M1 macrophages. MXF plus CPS also led to a reduction of proinflammatory cytokines and chemokines, including IL-6, TNF-α, IL-1β, IL-1α, CXCL-1, MCP-1, IL18, and IL33.</div></div><div><h3>Conclusion</h3><div>Our study showed that MXF plus CPS exhibited a protective effect on intestinal barrier damage due to abdominal infection under microgravity, potentially attributed to its anti-inflammatory properties and immune regulatory mechanisms. These findings may provide insights into the development of drugs targeting abdominal infections in t
{"title":"Moxifloxacin plus Cordyceps polysaccharide ameliorate intestinal barrier damage due to abdominal infection via anti-inflammation and immune regulation under simulated microgravity","authors":"Hong-Yun Nie , Jun Ge , Kai-Ge Liu , Yuan Yue , Hao Li , Hai-Guan Lin , Tao Zhang , Hong-Feng Yan , Bing-Xin Xu , Hong-Wei Sun , Jian-Wu Yang , Shao-Yan Si , Jin-Lian Zhou , Yan Cui","doi":"10.1016/j.lssr.2024.11.005","DOIUrl":"10.1016/j.lssr.2024.11.005","url":null,"abstract":"<div><h3>Background</h3><div>Currently, there is limited research on the impact of abdominal infection on intestinal damage under microgravity conditions. Cordyceps polysaccharide (CPS), the main active ingredient of Cordyceps, has demonstrated various pharmacological effects, including anti-inflammatory, antioxidant, and immunomodulatory properties. Moxifloxacin (MXF) is a fourth-generation quinolone antibiotic that is believed to have a dual regulatory effect on immune system activation and suppression. Our objective was to investigate the effects of MXF plus CPS on the intestinal barrier damage due to abdominal infection under microgravity.</div></div><div><h3>Methods</h3><div>The hindlimb unloading model in rats was employed to simulate microgravity. The rat model of abdominal infection was established by cecal ligation and puncture (CLP). MXF, CPS and the combination of the two drugs were used to treat CLP-rats in simulated microgravity. We assessed histopathological changes of ileum by hematoxylin and eosin staining. The intestinal ultrastructure was observed under transmission electron microscopy. Additionally, the expression of intestinal barrier proteins RegIII α/γ and MUC2 was detected by Western blot analysis, while the localization of these proteins within the ileum was examined using immunohistochemistry. Cytometric bead array (CBA) was employed to detect cytokine including IL-6, TNF-α, IL-1β, IL-1α, CXCL-1, MCP-1, IL-17A, IL-18, and IL-33. Flow cytometry analysis was conducted to determine the percentages of Treg cells, M1 macrophages, M2 macrophages, T cells and CD8<sup>+</sup><em>T</em> cells.</div></div><div><h3>Results</h3><div>The results showed that compared with the normal gravity groups, the simulated microgravity groups exhibited a significant decrease in RegIII α/γ protein expression, an increase in M1 macrophage frequency, and elevated levels of TNF-α, IL-1α, MCP-1 and IL-6. Notably, the combined application of MXF and CPS effectively mitigated intestinal barrier damage in CLP-rats exposed to microgravity, as evidenced by alleviated ultrastructural and pathological impairments in ileum, along with increased expression of key intestinal barrier proteins MUC2 and RegIII α/γ. Furthermore, the combination therapy enhances the proportion of T cells, CD8<sup>+</sup> <em>T</em> cells, and M2 macrophages in septic rats exposed to simulated microgravity while reducing the frequency of Treg cells and M1 macrophages. MXF plus CPS also led to a reduction of proinflammatory cytokines and chemokines, including IL-6, TNF-α, IL-1β, IL-1α, CXCL-1, MCP-1, IL18, and IL33.</div></div><div><h3>Conclusion</h3><div>Our study showed that MXF plus CPS exhibited a protective effect on intestinal barrier damage due to abdominal infection under microgravity, potentially attributed to its anti-inflammatory properties and immune regulatory mechanisms. These findings may provide insights into the development of drugs targeting abdominal infections in t","PeriodicalId":18029,"journal":{"name":"Life Sciences in Space Research","volume":"44 ","pages":"Pages 23-37"},"PeriodicalIF":2.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143047191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-01DOI: 10.1016/j.lssr.2024.12.001
A.S. Nemec-Bakk , V. Sridharan , J.S. Willey , I. Koturbash , D.K. Williams , M. Chesal , C.M. Patel , A.M. Borg , K. Reno , G. Gifford , W. Newhauser , J. Williams , J.C. Chancellor , M. Boerma
Future long duration space missions will expose astronauts to higher doses of galactic cosmic radiation (GCR) than those experienced on the international space station. Recent studies have demonstrated astronauts may be at risk for cardiovascular complications due to increased radiation exposure and fluid shift from microgravity. However, there is a lack of direct evidence on how the cardiovascular system is affected by GCR and microgravity since no astronauts have been exposed to exploratory mission relevant GCR doses. Therefore, we utilized a ground-based mouse model to determine the cardiovascular risks for space radiation exposure while the mice were simultaneously hindlimb suspended to mimic microgravity. 6-month-old male and female C57BL/6 mice were exposed to an absorbed dose of 0 Gy, 0.5 Gy, or 1.5 Gy simulated GCR (GCRsim) that comprised beams of 5 ions at NASA's Space Radiation Laboratory. Subcohorts of mice were hindlimb unloaded (HLU), starting 5 days before GCRsim until the completion of radiation exposure. GCRsim + HLU was performed over 8 hours (0.5 Gy) or 24 hours (1.5 Gy). After completion of GCRsim and HLU, mice were shipped to UAMS for long-term observation. Cardiac function was measured using high resolution ultrasound at 6 and 9 months after exposure. Tissues were collected after the final ultrasound and prepared for further analysis. Female mice exposed to 1.5 Gy + HLU demonstrated a significant increase in body weight compared to ground controls months after GCR exposure; however, there was no change in male body weights. Cardiac ultrasound revealed 0.5 Gy GCRsim decreased left ventricular (LV) mass, LV posterior wall thickness in diastole, and systole in males 6 months after exposure. In females, 1.5 Gy + HLU significantly increased LV posterior wall thickness in diastole and systole at 6 months. These changes in ultrasound measurements were no longer seen at 9 months. Moreover, at 9 months there was no change in total collagen content or density of the capillary network in the heart. Lastly, the combination of GCRsim and HLU influenced immune cell markers in the heart of female mice. These data suggest that combined simulated GCR and microgravity result in minor, yet statistically significant sex-dependent changes to body weight and cardiac structure.
{"title":"Sex-specific effects on the heart from combined exposure to simulated galactic cosmic radiation and hindlimb unloading","authors":"A.S. Nemec-Bakk , V. Sridharan , J.S. Willey , I. Koturbash , D.K. Williams , M. Chesal , C.M. Patel , A.M. Borg , K. Reno , G. Gifford , W. Newhauser , J. Williams , J.C. Chancellor , M. Boerma","doi":"10.1016/j.lssr.2024.12.001","DOIUrl":"10.1016/j.lssr.2024.12.001","url":null,"abstract":"<div><div>Future long duration space missions will expose astronauts to higher doses of galactic cosmic radiation (GCR) than those experienced on the international space station. Recent studies have demonstrated astronauts may be at risk for cardiovascular complications due to increased radiation exposure and fluid shift from microgravity. However, there is a lack of direct evidence on how the cardiovascular system is affected by GCR and microgravity since no astronauts have been exposed to exploratory mission relevant GCR doses. Therefore, we utilized a ground-based mouse model to determine the cardiovascular risks for space radiation exposure while the mice were simultaneously hindlimb suspended to mimic microgravity. 6-month-old male and female C57BL/6 mice were exposed to an absorbed dose of 0 Gy, 0.5 Gy, or 1.5 Gy simulated GCR (GCRsim) that comprised beams of 5 ions at NASA's Space Radiation Laboratory. Subcohorts of mice were hindlimb unloaded (HLU), starting 5 days before GCRsim until the completion of radiation exposure. GCRsim + HLU was performed over 8 hours (0.5 Gy) or 24 hours (1.5 Gy). After completion of GCRsim and HLU, mice were shipped to UAMS for long-term observation. Cardiac function was measured using high resolution ultrasound at 6 and 9 months after exposure. Tissues were collected after the final ultrasound and prepared for further analysis. Female mice exposed to 1.5 Gy + HLU demonstrated a significant increase in body weight compared to ground controls months after GCR exposure; however, there was no change in male body weights. Cardiac ultrasound revealed 0.5 Gy GCRsim decreased left ventricular (LV) mass, LV posterior wall thickness in diastole, and systole in males 6 months after exposure. In females, 1.5 Gy + HLU significantly increased LV posterior wall thickness in diastole and systole at 6 months. These changes in ultrasound measurements were no longer seen at 9 months. Moreover, at 9 months there was no change in total collagen content or density of the capillary network in the heart. Lastly, the combination of GCRsim and HLU influenced immune cell markers in the heart of female mice. These data suggest that combined simulated GCR and microgravity result in minor, yet statistically significant sex-dependent changes to body weight and cardiac structure.</div></div>","PeriodicalId":18029,"journal":{"name":"Life Sciences in Space Research","volume":"44 ","pages":"Pages 38-46"},"PeriodicalIF":2.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143047200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-01DOI: 10.1016/j.lssr.2024.08.006
V G Sowmeya, Mythili Sathiavelu
Microbial biofilms are universal. The intricate tapestry of biofilms has remarkable implications for the environment, health, and industrial processes. The field of space microbiology is actively investigating the effects of microgravity on microbes, and discoveries are constantly being made. Recent evidence suggests that extraterrestrial environments also fuel the biofilm formation. Understanding the biofilm mechanics under microgravitational conditions is crucial at this stage and could have an astounding impact on inter-planetary missions. This review systematically examines the existing understanding of biofilm development in space and provides insight into how molecules, physiology, or environmental factors influence biofilm formation during microgravitational conditions. In addition, biocontrol strategies targeting the formation and dispersal of biofilms in space environments are explored. In particular, the article highlights the potential benefits of using microbial biofilms in space for bioremediation, life support systems, and biomass production applications.
{"title":"Biofilm dynamics in space and their potential for sustainable space exploration – A comprehensive review","authors":"V G Sowmeya, Mythili Sathiavelu","doi":"10.1016/j.lssr.2024.08.006","DOIUrl":"10.1016/j.lssr.2024.08.006","url":null,"abstract":"<div><div>Microbial biofilms are universal. The intricate tapestry of biofilms has remarkable implications for the environment, health, and industrial processes. The field of space microbiology is actively investigating the effects of microgravity on microbes, and discoveries are constantly being made. Recent evidence suggests that extraterrestrial environments also fuel the biofilm formation. Understanding the biofilm mechanics under microgravitational conditions is crucial at this stage and could have an astounding impact on inter-planetary missions. This review systematically examines the existing understanding of biofilm development in space and provides insight into how molecules, physiology, or environmental factors influence biofilm formation during microgravitational conditions. In addition, biocontrol strategies targeting the formation and dispersal of biofilms in space environments are explored. In particular, the article highlights the potential benefits of using microbial biofilms in space for bioremediation, life support systems, and biomass production applications.</div></div>","PeriodicalId":18029,"journal":{"name":"Life Sciences in Space Research","volume":"44 ","pages":"Pages 108-121"},"PeriodicalIF":2.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142212530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-01DOI: 10.1016/j.lssr.2024.11.004
Ge Zhang, Lei Zhao, Zejun Li, Yeqing Sun
The space environment presents unique stressors, such as microgravity and space radiation, which can induce molecular and physiological changes in living organisms. To identify key reproducible transcriptomic features and explore potential biological roles in space-flown C. elegans, we integrated transcriptomic data from C. elegans subjected to four spaceflights aboard the International Space Station (ISS) and identified 32 reproducibly differentially expressed genes (DEGs). These DEGs were enriched in pathways related to the structural constituent of cuticle, defense response, unfolded protein response, longevity regulation, extracellular structural organization, and signal receptor regulation. Among these 32 DEGs, 13 genes were consistently downregulated across four spaceflight conditions, primarily associated with the structural constituent of the cuticle. The remaining genes, involved in defense response, unfolded protein response, and longevity regulation pathway, exhibited distinct patterns depending on spaceflight duration: they were downregulated during short-term spaceflights but upregulated during long-term spaceflights. To explore the potential space stressors responsible for these transcriptomic changes, we performed qRT-PCR experiments on C. elegans exposed to simulated microgravity and low-dose radiation. Our results demonstrated that cuticle-related gene expression was significantly downregulated under both simulated microgravity and low-dose radiation conditions. In contrast, almost all genes involved in defense response, unfolded protein response, and longevity regulation pathway were downregulated under simulated microgravity but upregulated under low-dose radiation exposure. These findings suggest that both microgravity and space radiation inhibit cuticle formation; microgravity as the primary stressor inhibit defense response, unfolded protein response, and longevity regulation pathway during short-term spaceflights, while space radiation may promote these processes during long-term spaceflights. In summary, through integrated spaceflight transcriptomic analyses and simulated space experiments, we identified key transcriptomic features and potential biological functions in space-flown C. elegans, shedding light on the space stressors responsible for these changes. This study provides new insights into the molecular and physiological adaptations of C. elegans to spaceflight, highlighting the distinct impacts of microgravity and space radiation.
{"title":"Integrated spaceflight transcriptomic analyses and simulated space experiments reveal key molecular features and functional changes driven by space stressors in space-flown C. elegans","authors":"Ge Zhang, Lei Zhao, Zejun Li, Yeqing Sun","doi":"10.1016/j.lssr.2024.11.004","DOIUrl":"10.1016/j.lssr.2024.11.004","url":null,"abstract":"<div><div>The space environment presents unique stressors, such as microgravity and space radiation, which can induce molecular and physiological changes in living organisms. To identify key reproducible transcriptomic features and explore potential biological roles in space-flown <em>C. elegans</em>, we integrated transcriptomic data from <em>C. elegans</em> subjected to four spaceflights aboard the International Space Station (ISS) and identified 32 reproducibly differentially expressed genes (DEGs). These DEGs were enriched in pathways related to the structural constituent of cuticle, defense response, unfolded protein response, longevity regulation, extracellular structural organization, and signal receptor regulation. Among these 32 DEGs, 13 genes were consistently downregulated across four spaceflight conditions, primarily associated with the structural constituent of the cuticle. The remaining genes, involved in defense response, unfolded protein response, and longevity regulation pathway, exhibited distinct patterns depending on spaceflight duration: they were downregulated during short-term spaceflights but upregulated during long-term spaceflights. To explore the potential space stressors responsible for these transcriptomic changes, we performed qRT-PCR experiments on <em>C. elegans</em> exposed to simulated microgravity and low-dose radiation. Our results demonstrated that cuticle-related gene expression was significantly downregulated under both simulated microgravity and low-dose radiation conditions. In contrast, almost all genes involved in defense response, unfolded protein response, and longevity regulation pathway were downregulated under simulated microgravity but upregulated under low-dose radiation exposure. These findings suggest that both microgravity and space radiation inhibit cuticle formation; microgravity as the primary stressor inhibit defense response, unfolded protein response, and longevity regulation pathway during short-term spaceflights, while space radiation may promote these processes during long-term spaceflights. In summary, through integrated spaceflight transcriptomic analyses and simulated space experiments, we identified key transcriptomic features and potential biological functions in space-flown <em>C. elegans</em>, shedding light on the space stressors responsible for these changes. This study provides new insights into the molecular and physiological adaptations of <em>C. elegans</em> to spaceflight, highlighting the distinct impacts of microgravity and space radiation.</div></div>","PeriodicalId":18029,"journal":{"name":"Life Sciences in Space Research","volume":"44 ","pages":"Pages 10-22"},"PeriodicalIF":2.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143047187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-01DOI: 10.1016/j.lssr.2024.10.007
Lin Cheng , Yitong Li , Jing Yan
Space life science has been a frontier discipline in the life sciences, aiming to study the life phenomena of earth organisms and their activity patterns under the special environment of space. This review summarizes studies in various key topics in space life science, namely, how microbiome changes in humans and plants, the development of space agriculture and the use of animal, plant and cell models to study the effect of space environments on physiology. We highlight the new possibilities of using high-quality protein crystals uniquely available when grown under space conditions to aid drug development on earth, and the state-of-the-art Bioregenerative Life Support Systems (BLSS) to achieve long term human survival in space.
{"title":"Space biological and human survival: Investigations into plants, animals, microorganisms and their components and bioregenerative life support systems","authors":"Lin Cheng , Yitong Li , Jing Yan","doi":"10.1016/j.lssr.2024.10.007","DOIUrl":"10.1016/j.lssr.2024.10.007","url":null,"abstract":"<div><div>Space life science has been a frontier discipline in the life sciences, aiming to study the life phenomena of earth organisms and their activity patterns under the special environment of space. This review summarizes studies in various key topics in space life science, namely, how microbiome changes in humans and plants, the development of space agriculture and the use of animal, plant and cell models to study the effect of space environments on physiology. We highlight the new possibilities of using high-quality protein crystals uniquely available when grown under space conditions to aid drug development on earth, and the state-of-the-art Bioregenerative Life Support Systems (BLSS) to achieve long term human survival in space.</div></div>","PeriodicalId":18029,"journal":{"name":"Life Sciences in Space Research","volume":"44 ","pages":"Pages 143-153"},"PeriodicalIF":2.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143047201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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