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The gut core microbial species Bifidobacterium longum: Colonization, mechanisms, and health benefits 肠道核心微生物物种长双歧杆菌:定植、机制和健康益处
IF 6.1 1区 生物学 Q1 MICROBIOLOGY Pub Date : 2024-11-07 DOI: 10.1016/j.micres.2024.127966
Yue Xiao , Lijuan Huang , Jianxin Zhao , Wei Chen , Wenwei Lu
Bifidobacterium longum (B. longum) is a species of the core microbiome in the human gut, whose abundance is closely associated with host age and health status. B. longum has been shown to modulate host gut microecology and have the potential to alleviate various diseases. Comprehensive understanding on the colonization mechanism of B. longum and mechanism of the host-B. longum interactions, can provide us possibility to prevent and treat human diseases through B. longum-directed strategies. In this review, we summarized the gut colonization characteristics of B. longum, discussed the diet factors that have ability/potential to enrich indigenous and/or ingested B. longum strains, and reviewed the intervention mechanisms of B. longum in multiple diseases. The key findings are as follows: First, B. longum has specialized colonization mechanisms, like a wide carbohydrate utilization spectrum that allows it to adapt to the host's diet, species-level conserved genes encoding bile salt hydrolase (BSHs), and appropriate bacterial surface structures. Second, dietary intervention (e.g., anthocyanins) could effectively improve the gut colonization of B. longum, demonstrating the feasibility of diet-tuned strain colonization. Finally, we analyzed the skewed abundance of B. longum in different types of diseases and summarized the main mechanisms by which B. longum alleviates digestive (repairing the intestinal mucosal barrier by stimulating Paneth cell activity), immune (up-regulating the regulatory T cell (Treg) populations and maintaining the balance of Th1/Th2), and neurological diseases (regulating the kynurenine pathway and quinolinic acid levels in the brain through the gut-brain axis).
长双歧杆菌(B. longum)是人类肠道核心微生物组的一个物种,其丰度与宿主的年龄和健康状况密切相关。研究表明,长双歧杆菌能调节宿主肠道微生态,并具有缓解各种疾病的潜力。全面了解长春花酵母菌的定植机制以及宿主与长春花酵母菌之间的相互作用机制,可为通过长春花酵母菌导向策略预防和治疗人类疾病提供可能。在这篇综述中,我们总结了长春花酵母菌的肠道定植特征,讨论了有能力/有潜力富集本地和/或摄入的长春花酵母菌菌株的饮食因素,并回顾了长春花酵母菌在多种疾病中的干预机制。主要发现如下:首先,长春芽孢杆菌具有专门的定植机制,如广泛的碳水化合物利用谱使其能够适应宿主的饮食、编码胆盐水解酶(BSHs)的物种级保守基因以及适当的细菌表面结构。其次,膳食干预(如花青素)可有效改善龙胆球菌的肠道定植,证明了膳食调整菌株定植的可行性。最后,我们分析了长春花酵母菌在不同类型疾病中的偏态丰度,并总结了长春花酵母菌缓解消化系统疾病(通过刺激Paneth细胞活性修复肠粘膜屏障)、免疫系统疾病(上调调节性T细胞(Treg)数量并维持Th1/Th2平衡)和神经系统疾病(通过肠脑轴调节脑内犬尿氨酸途径和喹啉酸水平)的主要机制。
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引用次数: 0
Extracellular Hsp90 of Candida albicans contributes to the virulence of the pathogen by activating the NF-κB signaling pathway and inducing macrophage pyroptosis 白色念珠菌的胞外 Hsp90 通过激活 NF-κB 信号通路和诱导巨噬细胞热毒症,增强了病原体的毒力。
IF 6.1 1区 生物学 Q1 MICROBIOLOGY Pub Date : 2024-11-05 DOI: 10.1016/j.micres.2024.127964
Ting Fang , Juan Xiong , Xin Huang , Xinyu Fang , Xuqing Shen , Yuanying Jiang , Hui Lu
Strategies aimed at targeting fungal extracellular heat shock protein 90 (eHsp90) using vaccines and antibodies have demonstrated encouraging potential in the prevention and management of invasive fungal diseases (IFDs). However, the precise underlying mechanism by which eHsp90 contributes to the heightened virulence of Candida albicans (C. albicans) remains an enigma, awaiting further elucidation. In our current research, we have found that the 47-kDa fragment of C. albicans Hsp90 (CaHsp90), which serves as the primary antigenic determinant, is not degraded within C. albicans cells. Moreover, we have discovered that extracellular CaHsp90 (eCaHsp90) is derived from the components of lysed C. albicans cells. We also generated recombinant CaHsp90 in Escherichia coli, and found that eCaHsp90 spreads beyond the initial C. albicans colonization site, thereby enhancing the overall virulence of the organism. Our results further clarify that eCaHsp90 activates the nuclear factor kappa-B (NF-κB) signaling pathway and upregulates the expression of NACHT, LRR, and PYD domains-containing protein 3 (NLRP3). This upregulation results in the activation of Gasdermin D (GSDMD) and subsequent macrophage pyroptosis, ultimately increasing the virulence of C. albicans. This study provides valuable insights into the mechanism by which eCaHsp90 contributes to the virulence of C. albicans, offering a pharmacological basis for antifungal strategies targeting fungal eHsp90.
利用疫苗和抗体靶向真菌细胞外热休克蛋白 90(eHsp90)的策略在预防和治疗侵袭性真菌疾病(IFDs)方面已显示出令人鼓舞的潜力。然而,eHsp90 导致白念珠菌(C. albicans)毒力增强的确切内在机制仍是一个谜,有待进一步阐明。在目前的研究中,我们发现作为主要抗原决定因子的白念珠菌 Hsp90(CaHsp90)47-kDa 片段在白念珠菌细胞内不会降解。此外,我们还发现细胞外 CaHsp90(eCaHsp90)来自裂解的白僵菌细胞成分。我们还在大肠杆菌中生成了重组 CaHsp90,并发现 eCaHsp90 会扩散到白僵菌最初的定植点之外,从而增强了该生物的整体毒力。我们的研究结果进一步阐明,eCaHsp90 可激活核因子卡巴-B(NF-κB)信号通路,并上调含 NACHT、LRR 和 PYD 结构域的蛋白 3(NLRP3)的表达。这种上调会导致加斯德敏 D(GSDMD)的活化和随后的巨噬细胞脓毒症,最终增强白僵菌的毒力。这项研究为了解 eCaHsp90 促进白僵菌毒力的机制提供了宝贵的见解,为针对真菌 eHsp90 的抗真菌策略提供了药理学基础。
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引用次数: 0
Cell-Penetrating Peptides in infection and immunization 细胞穿透肽在感染和免疫中的应用。
IF 6.1 1区 生物学 Q1 MICROBIOLOGY Pub Date : 2024-11-05 DOI: 10.1016/j.micres.2024.127963
Yongliang Du , Yan Xiong , Zhou Sha , Dong Guo , Beibei Fu , Xiaoyuan Lin , Haibo Wu
Bacteria and viruses pose significant threats to human health, as drug molecules and therapeutic agents are often hindered by cell membranes and tissue barriers from reaching intracellular targets. Cell-penetrating peptides (CPPs), composed of 5–30 amino acids, function as molecular shuttles that facilitate the translocation of therapeutic agents across biological barriers. Despite their therapeutic potential, CPPs exhibit limitations, such as insufficient cell specificity, low in vivo stability, reduced delivery efficiency, and limited tolerance under serum conditions. However, intelligent design and chemical modifications can enhance their cell penetration, stability, and selectivity. These advancements could significantly improve CPP-based drug delivery strategies, facilitating both infection treatment and immunization against bacterial and viral diseases. This review provides an overview of the applications of CPPs in various infections and immune diseases, summarizing their mechanisms and the challenges encountered during their application.
细菌和病毒对人类健康构成重大威胁,因为药物分子和治疗剂往往受到细胞膜和组织屏障的阻碍,无法到达细胞内靶点。细胞穿透肽(CPPs)由 5-30 个氨基酸组成,具有分子穿梭器的功能,可促进治疗药物穿越生物屏障。尽管具有治疗潜力,但 CPPs 也有其局限性,如细胞特异性不足、体内稳定性低、递送效率降低以及在血清条件下的耐受性有限。然而,智能设计和化学修饰可以增强其细胞穿透性、稳定性和选择性。这些进步可以大大改善基于 CPP 的给药策略,促进感染治疗和针对细菌和病毒疾病的免疫。本综述概述了 CPPs 在各种感染和免疫疾病中的应用,总结了它们的作用机制和应用过程中遇到的挑战。
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引用次数: 0
Development of a landing pad system for Aspergillus niger and its application in the overproduction of monacolin J 黑曲霉着陆垫系统的开发及其在过量生产莫纳可林中的应用 J.
IF 6.1 1区 生物学 Q1 MICROBIOLOGY Pub Date : 2024-11-02 DOI: 10.1016/j.micres.2024.127956
Linlin Yao , Junwei Zheng , Bin Wang , Li Pan
Aspergillus niger is a powerful and efficient cell factory, with the potential to synthesize valuable products as chassis cells. The use of microbial cell factories to produce monacolin J, a precursor for statin synthesis, as an alternative to chemical synthesis could meet increasing market demand. However, the need for precise large fragment gene editing and the availability of suitable integration loci hinders the application of this strain. Herein, we identified neutral integration sites of A. niger based on the combination of ATAC-seq, H3K4me3 epigenetic datasets. Next, a landing pad system was developed for the one-step integration of the MJ biosynthesis gene cluster (BGC) in A. niger. Furthermore, we optimized the precursor module supply, the auxiliary factor supply module of NADPH, the module for eliminating oxidative stress pressure, and the transporter module to improve the production of MJ. Finally, a multi-copy integration strategy was applied to the rapid integration of MJ BGC, achieving MJ titer up to 1851.52 mg/L at the 500 mL shaker level.
黑曲霉是一种强大而高效的细胞工厂,具有作为底盘细胞合成有价值产品的潜力。利用微生物细胞工厂生产他汀类药物合成的前体物质 Monacolin J,可替代化学合成,满足日益增长的市场需求。然而,精确的大片段基因编辑需求和合适整合位点的可用性阻碍了该菌株的应用。在此,我们结合 ATAC-seq、H3K4me3 表观遗传学数据集,确定了黑僵菌的中性整合位点。接下来,我们开发了一种着陆垫系统,用于一步整合黑僵菌中的 MJ 生物合成基因簇(BGC)。此外,我们还优化了前体模块供应、NADPH 辅助因子供应模块、消除氧化应激压力模块和转运模块,以提高 MJ 的产量。最后,我们采用多拷贝整合策略对 MJ BGC 进行了快速整合,在 500 mL 摇床水平上使 MJ 滴度达到 1851.52 mg/L。
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引用次数: 0
Quorum sensing: the “switch” in the competitive relationship between Gram-positive bacteria based on transcriptomic analysis 法定人数感应:基于转录组分析的革兰氏阳性细菌竞争关系中的 "开关"。
IF 6.1 1区 生物学 Q1 MICROBIOLOGY Pub Date : 2024-10-31 DOI: 10.1016/j.micres.2024.127961
Guoxu Ao , Changli Wang , Liguo Yang , Yue Ma , Zhaoxuan Wang , Yueqi Shi , Shanshan Sun , Wenxiang Ping
Competition phenomenon is widely presented in nature, however, few reports on the competition phenomenon between bacteria based on the perspective of quorum sensing (QS), especially between Gram-positive bacteria. Here, the Gram-positive bacteria Rhodococcus sp. HD1 and Microbacterium sp. HM-2 were co-cultured, and the epiphysiological indicators, transcriptomics combined with gene engineering technique were applied to clarify the role of QS in the competition between Gram-positive bacteria. The results showed that the morphology of strain HD1 was changed into ellipsoids from long rods, the surface-to-volume ratio increased, and the competition index increased within strains HM-2 and HD1. The biomass of strain HD1(8.06×107 CFU/mL) was decreased significantly (p<0.05) under co-culture system, compared with mono-culture (5.75×108 CFU/mL), indicating that strain HM-2 had an inhibitory effect on HD1 at 12 h. Transcriptomic analysis revealed that QS-related genes were highly expressed in strain HM-2, and the expression level of the virulence gene TM_0352 was the highest (FPKM: 1774.19). Meanwhile, the ABC transporters-related genes in strain HD1 were significantly increased. Furthermore, QS pathway-related genes in strain HM-2 and ABC transporters-related genes in strain HD1 showed a significant correlation with the gene TM_0352 expression by the Mantel test analysis (p<0.05), surmising that the TM_0352 gene played a dominant role in the co-culture system. Knockout and complementation experiments confirmed that the function of gene TM_0352. The structural equation model showed that the QS up-regulation of strain HM-2 significantly promoted the expression of virulence genes, while strain HD1 promoted ABC transporters to cope with the up-regulation of TM_0352. The up-regulation of TM_0352 promoted the biomass of strain HM-2 and inhibited the biomass of HD1.The above results displayed that the competition phenomenon appeared by QS driving the up-regulation of TM_0352 gene in strain HM-2, which led to the up-regulation of ABC transporters in strain HD1. And these findings provided new insights into the perspective of factors related to competition inhibition between bacteria.
竞争现象在自然界中广泛存在,但基于法定量感应(QS)视角的细菌间竞争现象,尤其是革兰氏阳性菌间竞争现象的报道却很少。本文以革兰氏阳性菌 Rhodococcus sp. HD1 和 Microbacterium sp. HM-2 为研究对象,应用表观生理指标、转录组学和基因工程技术,阐明了 QS 在革兰氏阳性菌竞争中的作用。结果表明,菌株HD1的形态由长杆变为椭圆形,表面体积比增加,菌株HM-2和HD1内部的竞争指数增加。与单株培养(5.75×108 CFU/mL)相比,共培养系统下菌株 HD1 的生物量(8.06×107 CFU/mL)显著下降(p<0.05),表明菌株 HM-2 在 12 h 内对 HD1 有抑制作用。转录组分析表明,QS相关基因在菌株HM-2中高表达,其中毒力基因TM_0352的表达量最高(FPKM:1774.19)。同时,菌株 HD1 中 ABC 转运体相关基因的表达量也明显增加。此外,经 Mantel 检验分析,菌株 HM-2 中的 QS 通路相关基因和菌株 HD1 中的 ABC 转运体相关基因与基因 TM_0352 的表达有明显的相关性(p
{"title":"Quorum sensing: the “switch” in the competitive relationship between Gram-positive bacteria based on transcriptomic analysis","authors":"Guoxu Ao ,&nbsp;Changli Wang ,&nbsp;Liguo Yang ,&nbsp;Yue Ma ,&nbsp;Zhaoxuan Wang ,&nbsp;Yueqi Shi ,&nbsp;Shanshan Sun ,&nbsp;Wenxiang Ping","doi":"10.1016/j.micres.2024.127961","DOIUrl":"10.1016/j.micres.2024.127961","url":null,"abstract":"<div><div>Competition phenomenon is widely presented in nature, however, few reports on the competition phenomenon between bacteria based on the perspective of quorum sensing (QS), especially between Gram-positive bacteria. Here, the Gram-positive bacteria <em>Rhodococcus</em> sp. HD1 and <em>Microbacterium</em> sp<em>.</em> HM-2 were co-cultured, and the epiphysiological indicators, transcriptomics combined with gene engineering technique were applied to clarify the role of QS in the competition between Gram-positive bacteria. The results showed that the morphology of strain HD1 was changed into ellipsoids from long rods, the surface-to-volume ratio increased, and the competition index increased within strains HM-2 and HD1. The biomass of strain HD1(8.06×10<sup>7</sup> CFU/mL) was decreased significantly (<em>p</em><0.05) under co-culture system, compared with mono-culture (5.75×10<sup>8</sup> CFU/mL), indicating that strain HM-2 had an inhibitory effect on HD1 at 12 h. Transcriptomic analysis revealed that QS-related genes were highly expressed in strain HM-2, and the expression level of the virulence gene <em>TM_0352</em> was the highest (FPKM: 1774.19). Meanwhile, the ABC transporters-related genes in strain HD1 were significantly increased. Furthermore, QS pathway-related genes in strain HM-2 and ABC transporters-related genes in strain HD1 showed a significant correlation with the gene <em>TM_0352</em> expression by the Mantel test analysis (<em>p</em>&lt;0.05), surmising that the <em>TM_0352</em> gene played a dominant role in the co-culture system. Knockout and complementation experiments confirmed that the function of gene <em>TM_0352</em>. The structural equation model showed that the QS up-regulation of strain HM-2 significantly promoted the expression of virulence genes, while strain HD1 promoted ABC transporters to cope with the up-regulation of <em>TM_0352</em>. The up-regulation of <em>TM_0352</em> promoted the biomass of strain HM-2 and inhibited the biomass of HD1.The above results displayed that the competition phenomenon appeared by QS driving the up-regulation of <em>TM_0352</em> gene in strain HM-2, which led to the up-regulation of ABC transporters in strain HD1. And these findings provided new insights into the perspective of factors related to competition inhibition between bacteria.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"290 ","pages":"Article 127961"},"PeriodicalIF":6.1,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142591183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Inhibitory effects of nitrite and sulfite/peroxymonosulfate on bacteria are mediated respectively through respiration and intracellular GSH homeostasis 亚硝酸盐和亚硫酸盐/过氧单硫酸盐对细菌的抑制作用分别通过呼吸作用和细胞内 GSH 平衡来介导。
IF 6.1 1区 生物学 Q1 MICROBIOLOGY Pub Date : 2024-10-31 DOI: 10.1016/j.micres.2024.127962
Yuxuan Liang , Xinyue Liu , Haozhen Chang , Jim Yap , Weining Sun , Haichun Gao
As nitrite, sulfite has been used in food preservation for centuries but how it inhibits bacterial growth remains underexplored. To address this issue, in this study, we set out to test if cytochrome (cyt) c proteins protect bacteria from the damage of certain reactive sulfur species (RSS) because they do so in the case of reactive nitrogen species (RNS). We show that some reactive sulfur species, such as sulfite and peroxymonosulfate (PMS), inhibit growth of bacterial strains devoid of cytochrome (cyt) c proteins. Subsequent investigations link the inhibition of sulfite/PMS to activity of cbb3-type heme-copper oxidase (cbb3-HCO). However, in vitro comparative analysis rules out that either cbb3-HCO or cyt bd oxidase is the primary target of sulfite/PMS. Instead, we found that sulfite/PMS and the cbb3-HCO loss regulate intracellular redox status in a similar manner, by affecting GSH/GSSG homeostasis. The link between the GSH/GSSG homeostasis and sulfite/PMS is further substantiated by using the mutants with enhanced GSSG generation. Furthermore, we present the data to show that inhibitory effects of nitrite and sulfite/PMS are additive although the overall effects may vary depending on species. Our results open an avenue to control bacteria by developing more robust agents that modulating intracellular redox status, which may be used in combination with nitrite as a promising antimicrobial strategy.
作为亚硝酸盐,亚硫酸盐在食品保鲜中的应用已有数百年历史,但它是如何抑制细菌生长的仍未得到充分研究。为了解决这个问题,在本研究中,我们着手测试细胞色素(cyt)c 蛋白是否能保护细菌免受某些活性硫物种(RSS)的损害,因为它们在活性氮物种(RNS)的情况下也能做到这一点。我们的研究表明,亚硫酸盐和过氧化单硫酸盐(PMS)等某些活性硫会抑制不含细胞色素(cyt)c 蛋白的细菌菌株的生长。随后的研究将亚硫酸盐/PMS 的抑制作用与 cbb3 型血红素铜氧化酶(cbb3-HCO)的活性联系起来。然而,体外比较分析排除了 cbb3-HCO 或细胞 bd 氧化酶是亚硫酸盐/PMS 的主要靶标的可能性。相反,我们发现亚硫酸盐/PMS 和 cbb3-HCO 损失通过影响 GSH/GSSG 平衡,以类似的方式调节细胞内氧化还原状态。通过使用 GSSG 生成增强的突变体,我们进一步证实了 GSH/GSSG 平衡与亚硫酸盐/PMS 之间的联系。此外,我们提供的数据表明,亚硝酸盐和亚硫酸盐/PMS 的抑制作用是相加的,但总体效果可能因物种而异。我们的研究结果为通过开发能调节细胞内氧化还原状态的更强效制剂来控制细菌开辟了一条途径,这种制剂可与亚硝酸盐结合使用,作为一种很有前景的抗菌策略。
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引用次数: 0
Probiotics: A potential strategy for improving diabetes mellitus complicated with cognitive impairment 益生菌:改善糖尿病并发认知障碍的潜在策略。
IF 6.1 1区 生物学 Q1 MICROBIOLOGY Pub Date : 2024-10-31 DOI: 10.1016/j.micres.2024.127960
Xin Shen , Feiyan Zhao , Zhixin Zhao , Jie Yu , Zhihong Sun
Diabetes mellitus (DM) is a common metabolic disease and one of the diseases with the highest number of complications at present. As the disease progresses, patients will gradually develop diabetes-related cognitive decline, mild cognitive impairment (MCI) or even dementia. The occurrence of diabetes-combined cognitive impairment undoubtedly imposes a heavy burden on patients and their families. Current research suggests that risk factors such as blood glucose levels, insulin resistance, oxidative stress and neuroinflammation have an important role in the development of diabetic cognitive impairment (DCI). With the development of technology and in-depth research, the relationship between the two-way communication between the gut and the brain has been gradually revealed, and more studies have found that the gut microbiota plays an important role in the development of DCI. This review explores the feasibility of probiotics as a potential strategy to assist in the improvement of DCI and its potential mechanisms from the perspective of the factors affecting DCI.
糖尿病(DM)是一种常见的代谢性疾病,也是目前并发症最多的疾病之一。随着病情的发展,患者会逐渐出现与糖尿病相关的认知功能下降、轻度认知障碍(MCI)甚至痴呆。糖尿病合并认知障碍的发生无疑给患者及其家庭带来了沉重的负担。目前的研究表明,血糖水平、胰岛素抵抗、氧化应激和神经炎症等危险因素在糖尿病认知障碍(DCI)的发生发展中起着重要作用。随着技术的发展和研究的深入,肠道与大脑之间的双向交流关系逐渐被揭示,越来越多的研究发现肠道微生物群在 DCI 的发生发展中扮演着重要角色。本综述从影响 DCI 的因素出发,探讨益生菌作为辅助改善 DCI 的潜在策略的可行性及其潜在机制。
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引用次数: 0
Transport of miR-766–3p to A549 cells by plasma-derived exosomes and its effect on intracellular survival of Mycobacterium tuberculosis by regulating NRAMP1 expression in A549 cells 血浆外泌体将 miR-766-3p 运送到 A549 细胞,并通过调节 A549 细胞中 NRAMP1 的表达影响结核分枝杆菌的胞内存活
IF 6.1 1区 生物学 Q1 MICROBIOLOGY Pub Date : 2024-10-31 DOI: 10.1016/j.micres.2024.127943
Xiaogang Cui , Fengfeng Zhang , Hangting Meng , Tianqi Yuan , Miao Li , Dan Yuan , Xiaoxia Fan , Xiaohui Jia , Quanhong Wang , Li Xing , Changxin Wu
Exosomal microRNAs (miRNAs) in circulation were recognized as potential biomarkers for the diagnosis of multiple diseases. However, its potential as a diagnostic hallmark for tuberculosis (TB) has yet to be explored. Here, we comprehensively analyze miRNA profiles in exosomes derived from the plasma of active TB patients and healthy persons to evaluate its efficacy in TB diagnosis. Small-RNA transcriptomic profiling analysis identified a total of 14 differentially expressed miRNAs (DEmiRNAs), among which the diagnostic potential of exosomal miR-766–3p, miR-376c-3p, miR-1283, and miR-125a-5p was evident from their respective areas under the ROC curve, which were 0.8963, 0.8313, 0.8097, and 0.8050, respectively. The bioinformatics analysis and Luciferase reporter assays confirmed that the 3′-untranslated region of natural resistance-associated macrophage protein 1 (NRAMP1) mRNA was targeted by miR-766–3p. The exosomes could be internalized by the A549 cells in co-culturing experiments. Furthermore, both increased miR-766–3p and decreased NRAMP1 expression were observed in Mtb-infected A549 cells. MiR-766–3p overexpression reduced the NRAMP1 levels, but increased intracellular Mtb, suggesting that miR-766–3p may facilitate Mtb survival by targeting NRAMP1. Moreover, miR-766–3p-transfected cells exhibited increased apoptosis and reduced proliferation following Mtb infection. Taken together, circulating exosomal miR-766–3p, miR-1283, miR-125a-5p, and miR-376c-3p may serve as candidate hallmarks for TB diagnosis where the presence of miR-766–3p seems associated with the vulnerability to Mtb infection in humans and could be a new molecular target for therapeutic intervention of TB.
循环中的外泌体微RNA(miRNA)被认为是诊断多种疾病的潜在生物标志物。然而,它作为结核病(TB)诊断标志物的潜力还有待探索。在这里,我们全面分析了从活动性肺结核患者和健康人血浆中提取的外泌体中的 miRNA 图谱,以评估其在肺结核诊断中的功效。小核糖核酸转录组分析共鉴定出 14 个差异表达的 miRNA(DEmiRNA),其中外泌体 miR-766-3p、miR-376c-3p、miR-1283 和 miR-125a-5p 的诊断潜力从它们各自的 ROC 曲线下面积(分别为 0.8963、0.8313、0.8097 和 0.8050)就可见一斑。生物信息学分析和荧光素酶报告实验证实,miR-766-3p靶向了天然抗药性相关巨噬细胞蛋白1(NRAMP1)mRNA的3′-非翻译区。在共培养实验中,外泌体可被 A549 细胞内化。此外,在受 Mtb 感染的 A549 细胞中观察到了 miR-766-3p 的增加和 NRAMP1 表达的减少。miR-766-3p的过表达降低了NRAMP1的水平,但增加了细胞内Mtb的水平,这表明miR-766-3p可能通过靶向NRAMP1来促进Mtb的存活。此外,miR-766-3p 转染的细胞在感染 Mtb 后凋亡增加,增殖减少。综上所述,循环外泌体 miR-766-3p、miR-1283、miR-125a-5p 和 miR-376c-3p 可作为结核病诊断的候选标志,其中 miR-766-3p 的存在似乎与人类易受 Mtb 感染有关,并可能成为结核病治疗干预的新分子靶点。
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引用次数: 0
Streptococcus anginosus orchestrates antibacterial potential of NETs facilitating survival of accompanying pathogens 副猪链球菌可协调 NET 的抗菌潜力,促进伴随病原体的存活。
IF 6.1 1区 生物学 Q1 MICROBIOLOGY Pub Date : 2024-10-30 DOI: 10.1016/j.micres.2024.127959
Magdalena Pilarczyk-Zurek , Joanna Budziaszek , Keerthanaa Nandagopal , Aleksandra Kurylek , Aleksandra Kozinska , Michal Dmowski , Izabela Sitkiewicz , Izabela Kern-Zdanowicz , Joanna Koziel
Streptococcus anginosus is considered an emerging opportunistic pathogen causing life-threatening infections, including abscesses and empyema. Noticeably, clinical data revealed that S. anginosus also constitutes an important component of polymicrobial infections. Here, we showed for the first time that S. anginosus inactivates the antibacterial potential of neutrophil extracellular traps (NETs). The process is determined by a cell wall-anchored nuclease referred to as SanA, which high expression dominates in clinical strains isolated from severe infections. Nuclease activity protects S. anginosus against the antibacterial activity of NETs, supporting at the same time the survival of coexisting highly pathogenic species of Enterobacteriales. Obtained data suggest that SanA nuclease should be recognized as a critical S. anginosus virulence factor determining severe monospecies purulent infections but also shielding other pathogens promoting the development of polymicrobial infections.
副猪链球菌被认为是一种新出现的机会性病原体,可导致危及生命的感染,包括脓肿和肺水肿。值得注意的是,临床数据显示,副猪链球菌也是多微生物感染的重要组成部分。在这里,我们首次发现,阴沟肠杆菌会使中性粒细胞胞外捕获物(NETs)的抗菌潜力失活。这一过程是由一种细胞壁锚定的核酸酶决定的,这种核酸酶被称为 SanA,在从严重感染中分离出的临床菌株中,SanA 的高表达占主导地位。核酸酶的活性能保护银环蛇菌抵御 NETs 的抗菌活性,同时支持共存的高致病性肠杆菌的生存。获得的数据表明,SanA 核酸酶应被视为阴沟肠杆菌的关键毒力因子,它不仅决定了严重的单菌种化脓性感染,还能保护其他病原体,促进多微生物感染的发展。
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引用次数: 0
Impact of arbuscular mycorrhizal fungi on maize rhizosphere microbiome stability under moderate drought conditions 在中等干旱条件下,丛枝菌根真菌对玉米根瘤微生物组稳定性的影响
IF 6.1 1区 生物学 Q1 MICROBIOLOGY Pub Date : 2024-10-30 DOI: 10.1016/j.micres.2024.127957
Yalin Chen , Chunyu Sun , Yuxin Yan , Dongxue Jiang , Shaoqi Huangfu , Lei Tian
With an alarming increase in global greenhouse gas emissions, unstable weather conditions are significantly impacting agricultural production. Drought stress is one of the frequent consequences of climate change that affects crop growth and yield. Addressing this issue is critical to ensure stable crop productivity under drought conditions. Arbuscular mycorrhizal fungi (AMF) establish symbiotic relationships with plants and enhance their resistance to adverse conditions. Effects of arbuscular mycorrhizal associations on the rhizosphere microbiome and root transcriptome under drought conditions have not been explored. Here, we investigated the effects of AMF and drought stress on rhizosphere microorganisms and root transcriptome of maize plants grown in chernozem soil. We used high-throughput sequencing data of bacterial 16S rRNA and fungal internal transcribed spacer regions (ITS) to identify rhizosphere microorganisms. Transcriptomic data were used to assess gene expression in maize plants under different treatments. Our results show that AMF maintains the composition of maize rhizosphere microorganisms under drought stress. In particular, the bacterial and fungal phyla maintained were Actinomycetes and Ascomycota, respectively. Transcriptomic data indicated that AMF influenced gene expression in maize plants under drought stress. Under drought stress, the expression of SWEET13, CHIT3, and RPL23A was significantly higher in the presence of AMF than it was without AMF inoculation, indicating better sugar transport, reduced malondialdehyde accumulation, and improved water use efficiency in AMF-inoculated maize plants. These findings suggest that AMF can enhance the resistance of maize to moderate drought stress by stabilising plant physical traits, which may help maintain the structure of the rhizosphere microbial community. This study provides valuable theoretical insights that should aid the utilization of AMF in sustainable agricultural practices.
随着全球温室气体排放量的惊人增长,不稳定的天气条件对农业生产产生了重大影响。干旱胁迫是气候变化影响作物生长和产量的常见后果之一。解决这一问题对于确保干旱条件下作物的稳定产量至关重要。丛枝菌根真菌(AMF)与植物建立共生关系,增强植物对不利条件的抵抗力。干旱条件下,丛枝菌根结合对根瘤微生物组和根转录组的影响尚未得到探讨。在此,我们研究了AMF和干旱胁迫对生长在切尔诺泽姆土壤中的玉米根瘤微生物和根转录组的影响。我们利用细菌 16S rRNA 和真菌内部转录间隔区(ITS)的高通量测序数据来鉴定根圈微生物。转录组数据用于评估不同处理条件下玉米植株的基因表达。我们的研究结果表明,在干旱胁迫下,AMF能维持玉米根瘤微生物的组成。特别是,所维持的细菌和真菌门分别是放线菌门和子囊菌门。转录组数据表明,AMF 影响了干旱胁迫下玉米植株的基因表达。在干旱胁迫下,AMF存在时,SWEET13、CHIT3和RPL23A的表达量明显高于未接种AMF时,这表明接种AMF的玉米植株糖分转运能力更强,丙二醛积累减少,水分利用效率提高。这些研究结果表明,AMF 可以通过稳定植物的物理性状来增强玉米对中度干旱胁迫的抵抗力,这可能有助于维持根圈微生物群落的结构。这项研究提供了宝贵的理论见解,有助于在可持续农业实践中利用 AMF。
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引用次数: 0
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Microbiological research
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