Pub Date : 2025-01-16Epub Date: 2024-12-17DOI: 10.1128/MRA.00836-23
Jessica M Lewis, Daniel K Arens, Abraham Quaye, Diana G Calvopina-Chavez, Kyson T Jensen, Ashley K Miller, Melinda M Moss, M Elizabeth Warren, Justina P Tavana, Steven M Johnson, Julianne H Grose
We announce the complete genomes of Klebsiella phages ValerieMcCarty03 and ValerieMcCarty04 isolated from wastewater treatment plant samples that infect a multidrug-resistant Klebsiella oxytoca clinical isolate. These phages belong to the T4-like cluster and fall within the Jiaodavirus genus.
{"title":"Genome sequences of two <i>Klebsiella</i> phages isolated from wastewater treatment samples that infect a clinical <i>Klebsiella</i> isolate.","authors":"Jessica M Lewis, Daniel K Arens, Abraham Quaye, Diana G Calvopina-Chavez, Kyson T Jensen, Ashley K Miller, Melinda M Moss, M Elizabeth Warren, Justina P Tavana, Steven M Johnson, Julianne H Grose","doi":"10.1128/MRA.00836-23","DOIUrl":"10.1128/MRA.00836-23","url":null,"abstract":"<p><p>We announce the complete genomes of <i>Klebsiella</i> phages ValerieMcCarty03 and ValerieMcCarty04 isolated from wastewater treatment plant samples that infect a multidrug-resistant <i>Klebsiella oxytoca</i> clinical isolate. These phages belong to the T4-like cluster and fall within the <i>Jiaodavirus</i> genus.</p>","PeriodicalId":18654,"journal":{"name":"Microbiology Resource Announcements","volume":" ","pages":"e0083623"},"PeriodicalIF":0.7,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11737154/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142837827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-16Epub Date: 2024-11-27DOI: 10.1128/mra.00725-24
Kan Iwata, Daisuke Minegishi, Mayumi Maeda, Kenji Maehashi, Jun Yoshikawa
In this study, a draft genome sequence of Zygosaccharomyces siamensis kiy1 isolated from unpasteurized honey in Japan was analyzed. This strain was reported to be a promising arabitol-producing strain, and this report contributes to a better understanding of arabitol production in yeasts.
{"title":"Draft genome sequence of <i>Zygosaccharomyces siamensis</i> kiy1 isolated from unpasteurized honey.","authors":"Kan Iwata, Daisuke Minegishi, Mayumi Maeda, Kenji Maehashi, Jun Yoshikawa","doi":"10.1128/mra.00725-24","DOIUrl":"10.1128/mra.00725-24","url":null,"abstract":"<p><p>In this study, a draft genome sequence of <i>Zygosaccharomyces siamensis</i> kiy1 isolated from unpasteurized honey in Japan was analyzed. This strain was reported to be a promising arabitol-producing strain, and this report contributes to a better understanding of arabitol production in yeasts.</p>","PeriodicalId":18654,"journal":{"name":"Microbiology Resource Announcements","volume":" ","pages":"e0072524"},"PeriodicalIF":0.7,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11737139/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142730437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Streptomyces are Gram-positive bacteria typically found in the soil, with very large genomes and high GC content, and are known to produce a wide range of secondary metabolites. We isolated and sequenced the genomes of three Streptomyces bacteria from the soil of soybean field in Tsuruoka, Japan.
{"title":"Genome sequences of three <i>Streptomyces</i> isolated from the soil of soybean field in Tsuruoka, Japan.","authors":"Ryuki Sato, Rena Saito, Tomoki Takeda, Nobuhiro Sasaki, Satoshi Yuzawa, Natsumi Saito, Kazuharu Arakawa","doi":"10.1128/mra.01059-24","DOIUrl":"10.1128/mra.01059-24","url":null,"abstract":"<p><p><i>Streptomyces</i> are Gram-positive bacteria typically found in the soil, with very large genomes and high GC content, and are known to produce a wide range of secondary metabolites. We isolated and sequenced the genomes of three <i>Streptomyces</i> bacteria from the soil of soybean field in Tsuruoka, Japan.</p>","PeriodicalId":18654,"journal":{"name":"Microbiology Resource Announcements","volume":" ","pages":"e0105924"},"PeriodicalIF":0.7,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11737033/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142730442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-16Epub Date: 2024-12-13DOI: 10.1128/mra.00585-24
Bojan Shutinoski, Annika Flint, Sandeep Tamber, Kelly Weedmark, Swapan Banerjee
Human enteric pathogens in fresh uncooked seafood are of concern to human health. Here, we report the complete genomes of bacteria not commonly found in freshly harvested seafood, two Salmonella Typhimurium strains and one draft Escherichia coli genome, isolated from harvested oysters grown in Canada.
{"title":"Complete genomes of <i>Salmonella</i> and draft genome of <i>Escherichia</i> isolates from oysters collected in Canada.","authors":"Bojan Shutinoski, Annika Flint, Sandeep Tamber, Kelly Weedmark, Swapan Banerjee","doi":"10.1128/mra.00585-24","DOIUrl":"10.1128/mra.00585-24","url":null,"abstract":"<p><p>Human enteric pathogens in fresh uncooked seafood are of concern to human health. Here, we report the complete genomes of bacteria not commonly found in freshly harvested seafood, two <i>Salmonella</i> Typhimurium strains and one draft <i>Escherichia coli</i> genome, isolated from harvested oysters grown in Canada.</p>","PeriodicalId":18654,"journal":{"name":"Microbiology Resource Announcements","volume":" ","pages":"e0058524"},"PeriodicalIF":0.7,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11737156/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142818622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-16Epub Date: 2024-12-16DOI: 10.1128/mra.00462-24
Eon-Min Ko, Seonghan Kim
In this study, we present whole genome sequences of Staphylococcus aureus RN4220. Using PacBio DNA sequencing and de novo assembly, we obtained a single contig of 2,697,647 bp, which includes 2,652 predicted genes comprising protein-coding sequences, tRNAs, and rRNAs.
{"title":"Genome sequences of <i>Staphylococcus aureus</i> RN4220.","authors":"Eon-Min Ko, Seonghan Kim","doi":"10.1128/mra.00462-24","DOIUrl":"10.1128/mra.00462-24","url":null,"abstract":"<p><p>In this study, we present whole genome sequences of <i>Staphylococcus aureus</i> RN4220. Using PacBio DNA sequencing and <i>de novo</i> assembly, we obtained a single contig of 2,697,647 bp, which includes 2,652 predicted genes comprising protein-coding sequences, tRNAs, and rRNAs.</p>","PeriodicalId":18654,"journal":{"name":"Microbiology Resource Announcements","volume":" ","pages":"e0046224"},"PeriodicalIF":0.7,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11737183/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142829354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-16Epub Date: 2024-12-12DOI: 10.1128/mra.00844-24
Fletcher Metz, Marco N Allemann, Joshua K Michener, Kevin S Myers, Fachuang Lu, Daniel R Noguera, Timothy J Donohue
A randomly barcoded transposon insertion sequencing (RB-TnSeq) library of Novosphingobium aromaticivorans DSM12444 was grown in media containing either glucose or the β-5-linked aromatic dimer dehydrodiconiferyl alcohol (DC-A) as the sole carbon source. The cultures were grown to saturation and then sequenced, yielding the barcode abundance data sets presented here.
{"title":"RB-TnSeq barcode abundance data sets for <i>Novosphingobium aromaticivorans</i> grown on the β-5-linked aromatic dimer dehydrodiconiferyl alcohol.","authors":"Fletcher Metz, Marco N Allemann, Joshua K Michener, Kevin S Myers, Fachuang Lu, Daniel R Noguera, Timothy J Donohue","doi":"10.1128/mra.00844-24","DOIUrl":"10.1128/mra.00844-24","url":null,"abstract":"<p><p>A randomly barcoded transposon insertion sequencing (RB-TnSeq) library of <i>Novosphingobium aromaticivorans</i> DSM12444 was grown in media containing either glucose or the β-5-linked aromatic dimer dehydrodiconiferyl alcohol (DC-A) as the sole carbon source. The cultures were grown to saturation and then sequenced, yielding the barcode abundance data sets presented here.</p>","PeriodicalId":18654,"journal":{"name":"Microbiology Resource Announcements","volume":" ","pages":"e0084424"},"PeriodicalIF":0.7,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11737130/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142813881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-16Epub Date: 2024-12-12DOI: 10.1128/mra.00793-24
Jung Hun Lee, Saba Yasmin, Nam-Hoon Kim, Chanyeong Jeong, Byeonghyeon Kang, Gwangje Lee, Dae-Wi Kim, Rabaab Zahra, Sang Hee Lee
We announce the complete genome of Escherichia coli strain PEC1003 belonging to ST7366, isolated from sewage water in Pakistan. This strain harbored a plasmid carrying various antibiotic resistance genes, including extended-spectrum β-lactamase (ESBL), blaCTX-M-15, emphasizing the management and surveillance of ESBL-producing E. coli in the environment of developing countries.
{"title":"Complete genome sequence of an <i>Escherichia coli</i> ST7366 strain harboring an extended-spectrum β-lactamase gene isolated from sewage water in Pakistan.","authors":"Jung Hun Lee, Saba Yasmin, Nam-Hoon Kim, Chanyeong Jeong, Byeonghyeon Kang, Gwangje Lee, Dae-Wi Kim, Rabaab Zahra, Sang Hee Lee","doi":"10.1128/mra.00793-24","DOIUrl":"10.1128/mra.00793-24","url":null,"abstract":"<p><p>We announce the complete genome of <i>Escherichia coli</i> strain PEC1003 belonging to ST7366, isolated from sewage water in Pakistan. This strain harbored a plasmid carrying various antibiotic resistance genes, including extended-spectrum β-lactamase (ESBL), <i>bla</i><sub>CTX-M-15</sub>, emphasizing the management and surveillance of ESBL-producing <i>E. coli</i> in the environment of developing countries.</p>","PeriodicalId":18654,"journal":{"name":"Microbiology Resource Announcements","volume":" ","pages":"e0079324"},"PeriodicalIF":0.7,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11737184/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142813876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-16Epub Date: 2024-11-29DOI: 10.1128/mra.00859-24
Emilia Andrea V Sabban, Adonis N Eclipse, Stephen Kyle C Arcan, Dexter Bryan L Esliza, Paul Jeremy C Lanete, Lindley C Susi, Gelito Joseph M Sikat
Complete genome sequences of six coliphages (genomic size: 87-347 Kbp; GC content: 35.10%-45.27%) belonging to the Felixounavirus, Tequintavirus, and Asteriusvirus genera were isolated from Philippine wastewater facilities and drainage systems. These phages offer potential for development as diagnostic and therapeutic tools for food and water safety.
{"title":"Complete genome sequences of six coliphages isolated from local water systems in the Philippines.","authors":"Emilia Andrea V Sabban, Adonis N Eclipse, Stephen Kyle C Arcan, Dexter Bryan L Esliza, Paul Jeremy C Lanete, Lindley C Susi, Gelito Joseph M Sikat","doi":"10.1128/mra.00859-24","DOIUrl":"10.1128/mra.00859-24","url":null,"abstract":"<p><p>Complete genome sequences of six coliphages (genomic size: 87-347 Kbp; GC content: 35.10%-45.27%) belonging to the <i>Felixounavirus</i>, <i>Tequintavirus</i>, and <i>Asteriusvirus</i> genera were isolated from Philippine wastewater facilities and drainage systems. These phages offer potential for development as diagnostic and therapeutic tools for food and water safety.</p>","PeriodicalId":18654,"journal":{"name":"Microbiology Resource Announcements","volume":" ","pages":"e0085924"},"PeriodicalIF":0.7,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11737134/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142751287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-16Epub Date: 2024-11-29DOI: 10.1128/mra.00726-24
Michaela A Murphy, D Garrett Brown, Rickesha S Bell, Allison M Weis, Logan A Barrios, W Zac Stephens, June L Round
Blautia is a genus of anaerobic, gram-positive bacteria commonly found in mammalian gastrointestinal tracts. Yet, how variations among different Blautia strains can impact host health is poorly understood. We present a Blautia sp. genome isolated from human feces whose supplementation to mice can ameliorate colitis severity and associated sociability deficits.
{"title":"Draft genome of a human gut-derived <i>Blautia</i> sp. that ameliorates colitis and colitis-associated sociability deficits in mice.","authors":"Michaela A Murphy, D Garrett Brown, Rickesha S Bell, Allison M Weis, Logan A Barrios, W Zac Stephens, June L Round","doi":"10.1128/mra.00726-24","DOIUrl":"10.1128/mra.00726-24","url":null,"abstract":"<p><p><i>Blautia</i> is a genus of anaerobic, gram-positive bacteria commonly found in mammalian gastrointestinal tracts. Yet, how variations among different <i>Blautia</i> strains can impact host health is poorly understood. We present a <i>Blautia</i> sp. genome isolated from human feces whose supplementation to mice can ameliorate colitis severity and associated sociability deficits.</p>","PeriodicalId":18654,"journal":{"name":"Microbiology Resource Announcements","volume":" ","pages":"e0072624"},"PeriodicalIF":0.7,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11737165/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142751341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dale Liebenberg, Nday L Sungu, Edith E Machowski, Christopher S Ealand, Bavesh D Kana
The complete genome sequences were determined for two mycobacteriophages isolated from Mycobacterium smegmatis mc2155. JayJay, a myoviral bacteriophage from cluster C1, encodes 234 putative genes, 32 tRNAs, and 1 tmRNA. Rinkes, a siphoviral bacteriophage from cluster B9, harbors a smaller genome encoding 93 putative genes.
{"title":"Complete genome sequences of mycobacteriophages JayJay and Rinkes.","authors":"Dale Liebenberg, Nday L Sungu, Edith E Machowski, Christopher S Ealand, Bavesh D Kana","doi":"10.1128/mra.01199-24","DOIUrl":"https://doi.org/10.1128/mra.01199-24","url":null,"abstract":"<p><p>The complete genome sequences were determined for two mycobacteriophages isolated from <i>Mycobacterium smegmatis</i> mc<sup>2</sup>155. JayJay, a <i>myoviral</i> bacteriophage from cluster C1, encodes 234 putative genes, 32 tRNAs, and 1 tmRNA. Rinkes, a <i>siphoviral</i> bacteriophage from cluster B9, harbors a smaller genome encoding 93 putative genes.</p>","PeriodicalId":18654,"journal":{"name":"Microbiology Resource Announcements","volume":" ","pages":"e0119924"},"PeriodicalIF":0.7,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142984299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}