Yenling Cho, Xue Chen, Lishan Huang, Xue Yang, Yuhua Wang
This study aimed to standardize the refractive measures using manifest refraction (MR) before and after using mydriatic eyedrops, and to explore the potential impact of age on the MR differences before mydriatic eyedrops (MRM), after rapid pupil dilation (MRRPD), and after slow pupil dilation (MRSPD). Three hundred sixty-two participants, aged 3–18 years were recruited from January 1, 2018, to September 30, 2018, using cluster sampling. MRM, MRRPD, and MRSPD were applied for measuring sphere and cylinder in the left and right eyes. At the initial inspection stage there were no significant differences between the MRM and MRRPD groups for sphere and cylinder values in the left and right eyes (P > 0 05). A significant difference was found for the sphere value in the left (P < 0 001) and right eyes (P < 0 001) among the MRM, MRRPD, and MRSPD groups, whereas there was no significant difference in cylinder value in the left (P = 0 691) and right eyes (P = 0 172) among groups at the review stage. The sphere value in the left and right eyes was significantly different among the MRM, MRRPD, and MRSPD groups for children aged 3–6 years, and there was a significant difference among groups for the sphere value in the right eye for children aged 7–11 years. There were no significant differences between the MRM and MRRPD groups in terms of sphere or cylinder values in the left and right eyes of children aged 12–18 years. The study findings indicate the results of sphere and cylinder measurements in the left and right eyes are consistent for MRM and MRRPD during the initial inspection stage, without any effect of the age of the children. However, the results of sphere measurements in the left and right eye were significantly different between the MRM, MRRPD, and MRSPD groups, especially for younger children.
{"title":"Potential for Standardization of Refractive Measures for Manifest Refraction Before and After Using Mydriatic Eyedrops in Children","authors":"Yenling Cho, Xue Chen, Lishan Huang, Xue Yang, Yuhua Wang","doi":"10.1166/nnl.2020.3140","DOIUrl":"https://doi.org/10.1166/nnl.2020.3140","url":null,"abstract":"This study aimed to standardize the refractive measures using manifest refraction (MR) before and after using mydriatic eyedrops, and to explore the potential impact of age on the MR differences before mydriatic eyedrops (MRM), after rapid pupil dilation (MRRPD), and after slow pupil\u0000 dilation (MRSPD). Three hundred sixty-two participants, aged 3–18 years were recruited from January 1, 2018, to September 30, 2018, using cluster sampling. MRM, MRRPD, and MRSPD were applied for measuring sphere and cylinder in the left and right eyes. At the initial inspection stage\u0000 there were no significant differences between the MRM and MRRPD groups for sphere and cylinder values in the left and right eyes (P > 0 05). A significant difference was found for the sphere value in the left (P < 0 001) and right eyes (P < 0 001) among the MRM,\u0000 MRRPD, and MRSPD groups, whereas there was no significant difference in cylinder value in the left (P = 0 691) and right eyes (P = 0 172) among groups at the review stage. The sphere value in the left and right eyes was significantly different among the MRM, MRRPD, and MRSPD\u0000 groups for children aged 3–6 years, and there was a significant difference among groups for the sphere value in the right eye for children aged 7–11 years. There were no significant differences between the MRM and MRRPD groups in terms of sphere or cylinder values in the left and\u0000 right eyes of children aged 12–18 years. The study findings indicate the results of sphere and cylinder measurements in the left and right eyes are consistent for MRM and MRRPD during the initial inspection stage, without any effect of the age of the children. However, the results of\u0000 sphere measurements in the left and right eye were significantly different between the MRM, MRRPD, and MRSPD groups, especially for younger children.","PeriodicalId":18871,"journal":{"name":"Nanoscience and Nanotechnology Letters","volume":"12 1","pages":"563-569"},"PeriodicalIF":0.0,"publicationDate":"2020-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44298653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
LiTFSI/EMITFSI ionic liquid was applied in this study as an electrolyte to enhance electrochemical performances of LiNi0.8 Co0.15 Al0.05 O2 /Li4 Ti5 O12 (NCA/LTO) batteries at a high charging cutoff voltage of 3.2 V. Li-EMITFSI electrolyte generated extremely stable and uniform cathode electrolyte interface (CEI) nano film on the cathode surface. This CEI film not only inhibited the continuous decomposition of electrolyte, but also stabilized the high operating voltage of NCA/LTO batteries, resulting in enhanced discharge gravimetric specific capacity (Cg) and cyclic stability of NCA/LTO batteries. With Li-EMITFSI electrolyte, the NCA/LTO batteries achieved higher C g of 172 mAhg –1 at 0.1 C and good capacity retention of 75% over 50 cycles at 1.4 ~ 3.2 V, compared to traditional commercial electrolytes.
{"title":"Boosting Electrochemical Performances of High-Voltage NCA/LTO Cells by Cathode Electrolyte Interface Nano Film Formation in Ionic Liquid Electrolyte","authors":"Hongquan Gao, Jiaxin Peng, Wudan Cheng, Haoqian Guo, Guijiang Xu, Haitao Zhou, Jianchun Wu, Shu-Wei Hong, Jian-hong Yang","doi":"10.1166/nnl.2020.3142","DOIUrl":"https://doi.org/10.1166/nnl.2020.3142","url":null,"abstract":"LiTFSI/EMITFSI ionic liquid was applied in this study as an electrolyte to enhance electrochemical performances of LiNi0.8 Co0.15 Al0.05 O2 /Li4 Ti5 O12 (NCA/LTO) batteries at a high charging cutoff voltage\u0000 of 3.2 V. Li-EMITFSI electrolyte generated extremely stable and uniform cathode electrolyte interface (CEI) nano film on the cathode surface. This CEI film not only inhibited the continuous decomposition of electrolyte, but also stabilized the high operating voltage of NCA/LTO batteries, resulting\u0000 in enhanced discharge gravimetric specific capacity (Cg) and cyclic stability of NCA/LTO batteries. With Li-EMITFSI electrolyte, the NCA/LTO batteries achieved higher C g of 172 mAhg –1 at 0.1 C and good capacity retention of 75% over 50 cycles at\u0000 1.4 ~ 3.2 V, compared to traditional commercial electrolytes.","PeriodicalId":18871,"journal":{"name":"Nanoscience and Nanotechnology Letters","volume":"12 1","pages":"467-475"},"PeriodicalIF":0.0,"publicationDate":"2020-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44633257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Glioma is a malignant tumor that originates in the brain and accounts for more than half of all brain tumors. The disease is associated with a high fatality rate. Satisfactory treatment cannot be achieved through conventional craniotomy. We use carboxymethyl chitosan-coated Fe3 O4 composite nanoparticles (CMCS-Fe3 O4 NPs) as a drug delivery platform, and installed transferrin (TRF) with a targeting function on the platform. The anticancer drug methotrexate (MTX) is loaded onto the composite nanomaterial to complete a new delivery system for nano-drug targeted treatment of glioma. The composite nanoparticle is not toxic to cells in vitro, but after loading with it is loaded with MTX, it significantly inhibits proliferation of C6 cells. In vivo experiments show that CMCS-Fe3 O4NPs-TRF-MTX has a marked therapeutic effect on glioma in mouse models. The nano complex quickly penetrates the blood-brain barrier and enters brain tissues. An aggregation effect is not obvious. MRI monitoring for up to 4.0 h shows that CMCS-Fe3 O4 NPs-TRF-MTX slowly releases drug molecules over an extended period, thus increasing the duration of drug activity.
{"title":"Experimental Study on Targeting Brain Tumor with Ultrasound Nanoparticles Across Blood Brain Barrier","authors":"Yu-guang Liu, Hua Gao, Qisheng Hu, Puying An","doi":"10.1166/nnl.2020.3129","DOIUrl":"https://doi.org/10.1166/nnl.2020.3129","url":null,"abstract":"Glioma is a malignant tumor that originates in the brain and accounts for more than half of all brain tumors. The disease is associated with a high fatality rate. Satisfactory treatment cannot be achieved through conventional craniotomy. We use carboxymethyl chitosan-coated Fe3\u0000 O4 composite nanoparticles (CMCS-Fe3 O4 NPs) as a drug delivery platform, and installed transferrin (TRF) with a targeting function on the platform. The anticancer drug methotrexate (MTX) is loaded onto the composite nanomaterial to complete a new delivery\u0000 system for nano-drug targeted treatment of glioma. The composite nanoparticle is not toxic to cells in vitro, but after loading with it is loaded with MTX, it significantly inhibits proliferation of C6 cells. In vivo experiments show that CMCS-Fe3 O4NPs-TRF-MTX\u0000 has a marked therapeutic effect on glioma in mouse models. The nano complex quickly penetrates the blood-brain barrier and enters brain tissues. An aggregation effect is not obvious. MRI monitoring for up to 4.0 h shows that CMCS-Fe3 O4 NPs-TRF-MTX slowly releases drug\u0000 molecules over an extended period, thus increasing the duration of drug activity.","PeriodicalId":18871,"journal":{"name":"Nanoscience and Nanotechnology Letters","volume":"12 1","pages":"543-549"},"PeriodicalIF":0.0,"publicationDate":"2020-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46588339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Gastric cancer is a malignant tumor that is originated from the epithelia of the gastric mucosa. Although the gastroscopic biopsy of suspicious gastric areas can provide better early-warning opportunities for patients with malignant tumors that result in achieving early diagnosis and treatment, many malignant tumors are still excluded due to atypical histology, sampling errors, nonspecific antibodies, and other reasons, which pose a threat to the physical and mental health of patients. Therefore, more sensitive and specific detection methods of gastric cancer are needed to improve the screening efficiency of gastroscopic biopsy. The sensitivity of nano PCR was 10 times higher than that of conventional PCR. In comparison with the conventional RT-PCR method, nano PCR technology can amplify brighter bands and identify higher gene expression levels for ALK weak positive gastric cancer in IHC, and improve the detection rate of clinical specimens with lower ALK staining. Therefore, nano-gold polymerase chain reaction used gold nanoparticle (nano-gold PCR) has high sensitivity and positive detection rate for ALK-positive gastric cancer identified by gastroscopy. When a low concentration was observed for the amplified gene, especially when the biopsy tissue was too small to carry out IHC staining, the target gene could be amplified more effectively. Therefore, nano PCR technology is proposed to be widely used in target gene detection of biopsy tissue to achieve better tumor screening.
{"title":"Highly Sensitive Gold Nanoparticle Polymerase Chain Reaction in the Detection of Anaplastic Lymphoma Kinase-Positive Gastric Cancer from a Biopsy Specimen","authors":"Dan Zhang, Zhaoxi Lu, Bing Sun","doi":"10.1166/nnl.2020.3128","DOIUrl":"https://doi.org/10.1166/nnl.2020.3128","url":null,"abstract":"Gastric cancer is a malignant tumor that is originated from the epithelia of the gastric mucosa. Although the gastroscopic biopsy of suspicious gastric areas can provide better early-warning opportunities for patients with malignant tumors that result in achieving early diagnosis and\u0000 treatment, many malignant tumors are still excluded due to atypical histology, sampling errors, nonspecific antibodies, and other reasons, which pose a threat to the physical and mental health of patients. Therefore, more sensitive and specific detection methods of gastric cancer are needed\u0000 to improve the screening efficiency of gastroscopic biopsy. The sensitivity of nano PCR was 10 times higher than that of conventional PCR. In comparison with the conventional RT-PCR method, nano PCR technology can amplify brighter bands and identify higher gene expression levels for ALK weak\u0000 positive gastric cancer in IHC, and improve the detection rate of clinical specimens with lower ALK staining. Therefore, nano-gold polymerase chain reaction used gold nanoparticle (nano-gold PCR) has high sensitivity and positive detection rate for ALK-positive gastric cancer identified by\u0000 gastroscopy. When a low concentration was observed for the amplified gene, especially when the biopsy tissue was too small to carry out IHC staining, the target gene could be amplified more effectively. Therefore, nano PCR technology is proposed to be widely used in target gene detection of\u0000 biopsy tissue to achieve better tumor screening.","PeriodicalId":18871,"journal":{"name":"Nanoscience and Nanotechnology Letters","volume":"12 1","pages":"498-505"},"PeriodicalIF":0.0,"publicationDate":"2020-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43901030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Anesthesia for upper limb surgery is commonly used in clinical practice. At present, the traditional method uses anatomical localization for nerve block, which can result incomplete local block and reduced anesthetic effect. Instead, ultrasound-guided nerve block can help clinicians intuitively grasp structure of patient tissues and provide a real-time display of needle penetration. Propofol is the most commonly used intravenous anesthetic in the world, but its use is still limited. In order to improve the limitations of this drug, a propofol nanoemulsion was developed and used in combination with a lower brachial plexus block. In this study, 100 patients were randomly divided into two groups, 50 in each group. The propofol nanoinjection for nerve block was used in the experimental group and ketamine was used in the control group. The quality of the operation, respiratory inhibition/asphyxia, recovery time, OPS score, agitation score, and complications were compared between the two groups. There was no significant difference in blood loss between the two groups (P > 0 05). The experimental results showed that the lower brachial plexus block and propofol nanoemulsion provided a better analgesic effect than ketamine and propofol nanoemulsion in the operation.
{"title":"Anesthesia Effect of Propofol Nanoinjection Combined with Nerve Block","authors":"Jie Luo, Deming Wang, Wenqiang Guo, Hong Cao","doi":"10.1166/nnl.2020.3130","DOIUrl":"https://doi.org/10.1166/nnl.2020.3130","url":null,"abstract":"Anesthesia for upper limb surgery is commonly used in clinical practice. At present, the traditional method uses anatomical localization for nerve block, which can result incomplete local block and reduced anesthetic effect. Instead, ultrasound-guided nerve block can help clinicians\u0000 intuitively grasp structure of patient tissues and provide a real-time display of needle penetration. Propofol is the most commonly used intravenous anesthetic in the world, but its use is still limited. In order to improve the limitations of this drug, a propofol nanoemulsion was developed\u0000 and used in combination with a lower brachial plexus block. In this study, 100 patients were randomly divided into two groups, 50 in each group. The propofol nanoinjection for nerve block was used in the experimental group and ketamine was used in the control group. The quality of the operation,\u0000 respiratory inhibition/asphyxia, recovery time, OPS score, agitation score, and complications were compared between the two groups. There was no significant difference in blood loss between the two groups (P > 0 05). The experimental results showed that the lower brachial plexus block and\u0000 propofol nanoemulsion provided a better analgesic effect than ketamine and propofol nanoemulsion in the operation.","PeriodicalId":18871,"journal":{"name":"Nanoscience and Nanotechnology Letters","volume":"12 1","pages":"512-517"},"PeriodicalIF":0.0,"publicationDate":"2020-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48598827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ZEB1-AS1 is an Long non-coding RNA (lncRNA) that is abnormally expressed in various tumor types and affects tumor etiology and progression. ZEB1-AS1 can affect the malignant progression of gastric cancer through the miRNA-200b/Wnt1 axis. However, its effect on proliferation, invasion, and apoptosis of gastric cancer is unclear. Using BGC-803 cells, we studied the effects of lncRNA ZEB1-AS1 and miRNA-200b on the proliferation, invasion, and apoptosis of gastric cancer. Using qRT-PCR, we found that the expression of ZEB1-AS1 was significantly increased and the expression of miRNA-200b was significantly decreased in BGC-803 cells. By CCK-8, Transwell, and flow cytometry assays, we also found that down-regulation of ZEB1-AS1 and upregulation of miRNA-200b inhibited the proliferation and invasion of BGC-803 cells and promoted apoptosis. Furthermore, upregulation of ZEB1-AS1 and miRNA-200b reversed these effects. LncRNA ZEB1-AS1 inhibits the proliferation and invasion of gastric cancer cells and induces apoptosis by regulating the miRNA-200b/Wnt1 molecular axis.
{"title":"Role of Long Non-Coding RNA Zinc Finger E-Box Binding Homeobox 1 Antisense 1 in Regulating the microRNAs-200b/Wnt1 Axis Effects on Proliferation, Invasion, and Apoptosis of Gastric Carcinoma Cells","authors":"C. Mi, Li Ren, Dan Zhang, Shuixiang He","doi":"10.1166/nnl.2020.3135","DOIUrl":"https://doi.org/10.1166/nnl.2020.3135","url":null,"abstract":"ZEB1-AS1 is an Long non-coding RNA (lncRNA) that is abnormally expressed in various tumor types and affects tumor etiology and progression. ZEB1-AS1 can affect the malignant progression of gastric cancer through the miRNA-200b/Wnt1 axis. However, its effect on proliferation, invasion,\u0000 and apoptosis of gastric cancer is unclear. Using BGC-803 cells, we studied the effects of lncRNA ZEB1-AS1 and miRNA-200b on the proliferation, invasion, and apoptosis of gastric cancer. Using qRT-PCR, we found that the expression of ZEB1-AS1 was significantly increased and the expression\u0000 of miRNA-200b was significantly decreased in BGC-803 cells. By CCK-8, Transwell, and flow cytometry assays, we also found that down-regulation of ZEB1-AS1 and upregulation of miRNA-200b inhibited the proliferation and invasion of BGC-803 cells and promoted apoptosis. Furthermore, upregulation\u0000 of ZEB1-AS1 and miRNA-200b reversed these effects. LncRNA ZEB1-AS1 inhibits the proliferation and invasion of gastric cancer cells and induces apoptosis by regulating the miRNA-200b/Wnt1 molecular axis.","PeriodicalId":18871,"journal":{"name":"Nanoscience and Nanotechnology Letters","volume":"12 1","pages":"556-562"},"PeriodicalIF":0.0,"publicationDate":"2020-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47248825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ling Zhao, Li-Hai Tan, Guoliang Cheng, Yu Miao, Xiang-ming Zhou, Shigang Du, Jia Wang, Yang Yang, Ke Tingyu
The present work selected 120 patients, affected by diabetes, as control subjects in the Affiliated Hospital of Kunming Medical University. According to the principle of random distribution, two groups were established, miglitol study group (65 cases) and metformin control group (55 cases). The Ag + disinfectant sampling needle is a rapid detection tool to evaluate the efficacy of diabetes in both groups. The related biochemical indexes of the patients were measured before and after treatment at the 3rd, 6th, and 12th weeks. The test results showed that there were significant differences in fasting blood glucose value (FBG) and postprandial blood glucose value (2hFBG) among the two groups after 3-, 6-, and 12-month treatment. With the extension of treatment, the blood glucose level of diabetic patients gradually stabilized. Fasting insulin level (FINS) and postprandial two-hour insulin level (2hINS) increased gradually. At the 12th week, by measuring the HbA1c level of the patients, it was found decreasing significantly. In detail, the study group decreased more significantly than the control group, and the incidence of adverse reactions during treatment was 10.8% and 23.6%, respectively, with a significant statistical significance (P < 0 05). Meanwhile, we measured other biochemical indexes of patients from both groups after drug treatment. At the 6th week, the levels of glycosylated glycoprotein (HbA1c), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C) in the study group decreased more than in the control group. Therefore, miglitol shows a better hypoglycemic effect on type 2 diabetes, and the gastrointestinal adverse reactions are lesser than metformin, which is more worthy in clinical application.
{"title":"Analysis of the Effect of Miglitol in the Treatment of Diabetes Mellitus Based on Ag+ Disinfectant Sampling Needle","authors":"Ling Zhao, Li-Hai Tan, Guoliang Cheng, Yu Miao, Xiang-ming Zhou, Shigang Du, Jia Wang, Yang Yang, Ke Tingyu","doi":"10.1166/nnl.2020.3132","DOIUrl":"https://doi.org/10.1166/nnl.2020.3132","url":null,"abstract":"The present work selected 120 patients, affected by diabetes, as control subjects in the Affiliated Hospital of Kunming Medical University. According to the principle of random distribution, two groups were established, miglitol study group (65 cases) and metformin control group (55\u0000 cases). The Ag + disinfectant sampling needle is a rapid detection tool to evaluate the efficacy of diabetes in both groups. The related biochemical indexes of the patients were measured before and after treatment at the 3rd, 6th, and 12th weeks. The test results showed that there were significant\u0000 differences in fasting blood glucose value (FBG) and postprandial blood glucose value (2hFBG) among the two groups after 3-, 6-, and 12-month treatment. With the extension of treatment, the blood glucose level of diabetic patients gradually stabilized. Fasting insulin level (FINS) and postprandial\u0000 two-hour insulin level (2hINS) increased gradually. At the 12th week, by measuring the HbA1c level of the patients, it was found decreasing significantly. In detail, the study group decreased more significantly than the control group, and the incidence of adverse reactions during treatment\u0000 was 10.8% and 23.6%, respectively, with a significant statistical significance (P < 0 05). Meanwhile, we measured other biochemical indexes of patients from both groups after drug treatment. At the 6th week, the levels of glycosylated glycoprotein (HbA1c), triglyceride (TG), high-density\u0000 lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C) in the study group decreased more than in the control group. Therefore, miglitol shows a better hypoglycemic effect on type 2 diabetes, and the gastrointestinal adverse reactions are lesser than metformin, which\u0000 is more worthy in clinical application.","PeriodicalId":18871,"journal":{"name":"Nanoscience and Nanotechnology Letters","volume":"12 1","pages":"550-555"},"PeriodicalIF":0.0,"publicationDate":"2020-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47707762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study was established to investigate the effects of cisplatin nano-liposomes on the apoptosis of the human retinoblastoma (RB) cell line Y79 in vitro and in vivo. Y79 cells were cultured and then exposed to Annexin V/PI to test their apoptosis, tested with the Caspase-3 activity detection kit to examine the change in activity of Caspase-3, and subjected to western blotting to test Bcl-2 and Bax protein expression. Y79-cell-transplanted tumor model in nude mice was also established and divided into three groups, with five nude mice in each. Cisplatin nano-liposomes were applied to the experimental group, cisplatin was injected into the control group, while saline was administered to the blank group, after which the nude mice were killed and the tumor was removed. Tumor volumes and weights in the three groups were compared. Nucleic acid extraction from magnetic beads was adopted to extract DNA, RT-PCR was employed to test Bcl-2 and Bax mRNA levels in tumor tissues, and in situ cell death assay kit was applied to test apoptotic cells. In comparison to the cisplatin solution and DMSO groups, the cisplatin liposome group showed higher Y79 apoptotic rate, Caspase-3 activity, and Bax protein expression, and lower Bcl-2 protein expression (all P < 0 05). In comparison with the control and blank groups, the experimental group showed lower tumor volume, weight, and Bcl-2 mRNA level of nude mice. In addition, in comparison with the control group, the experimental group showed higher cellular apoptotic rate and Bax mRNA level. In terms of the clinical effects of cisplatin nano-liposomes on a tumor transplant in nude mice with cervical cancer, they were shown to promote tumor apoptosis.
{"title":"Cisplatin Nano-Liposomes Promoting Apoptosis of Retinoblastoma Cells Both In Vivo and In Vitro","authors":"Li-juan Zhao, Fei Wang, W. Fan","doi":"10.1166/nnl.2020.3127","DOIUrl":"https://doi.org/10.1166/nnl.2020.3127","url":null,"abstract":"This study was established to investigate the effects of cisplatin nano-liposomes on the apoptosis of the human retinoblastoma (RB) cell line Y79 in vitro and in vivo. Y79 cells were cultured and then exposed to Annexin V/PI to test their apoptosis, tested with the Caspase-3\u0000 activity detection kit to examine the change in activity of Caspase-3, and subjected to western blotting to test Bcl-2 and Bax protein expression. Y79-cell-transplanted tumor model in nude mice was also established and divided into three groups, with five nude mice in each. Cisplatin nano-liposomes\u0000 were applied to the experimental group, cisplatin was injected into the control group, while saline was administered to the blank group, after which the nude mice were killed and the tumor was removed. Tumor volumes and weights in the three groups were compared. Nucleic acid extraction from\u0000 magnetic beads was adopted to extract DNA, RT-PCR was employed to test Bcl-2 and Bax mRNA levels in tumor tissues, and in situ cell death assay kit was applied to test apoptotic cells. In comparison to the cisplatin solution and DMSO groups, the cisplatin liposome group showed higher\u0000 Y79 apoptotic rate, Caspase-3 activity, and Bax protein expression, and lower Bcl-2 protein expression (all P < 0 05). In comparison with the control and blank groups, the experimental group showed lower tumor volume, weight, and Bcl-2 mRNA level of nude mice. In addition, in comparison\u0000 with the control group, the experimental group showed higher cellular apoptotic rate and Bax mRNA level. In terms of the clinical effects of cisplatin nano-liposomes on a tumor transplant in nude mice with cervical cancer, they were shown to promote tumor apoptosis.","PeriodicalId":18871,"journal":{"name":"Nanoscience and Nanotechnology Letters","volume":"12 1","pages":"536-542"},"PeriodicalIF":0.0,"publicationDate":"2020-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47122575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. You, Jia Song, Z. Wang, Bei Wang, Jingsheng Liu
There was inefficient light absorption in the thin active layers due to optical losses in Organic Solar Cells (OSCs) with relatively large area. Therefore, it is a key issue to have a light trapping structure for highly efficient OSCs. For high performance devices fabrication, a smart grating was fabricated using holographic photolithography incorporated with wet etching technology. Scanning electron microscopy (SEM) images of fabrication were employed before/after spin-coating active layer. With the aid of optical finite difference time Domain (FDTD) simulation for optical effect, the optimized device structure ITO (1D grating)/PEDOT:PSS (40 nm)/PBDB-T:ITIC (100 nm)/PDINO (5 nm)/Al (100 nm) was obtained. The experimental results showed that when the grating period was 350 nm, depth 40 nm, the power conversion efficiencies (PCE) reached to 9.51%, an apparent increase from those of the typical P3HT:PC71BM structure. This work indicates that the diffraction gratings had a potential to realize more efficient organic photovoltaics, if suitable fabrication processing methods can be developed.
{"title":"Efficiency Improvement of Organic Solar Cells Using 350 nm Surface Relief Grating by Holographic Lithography","authors":"M. You, Jia Song, Z. Wang, Bei Wang, Jingsheng Liu","doi":"10.1166/nnl.2020.3134","DOIUrl":"https://doi.org/10.1166/nnl.2020.3134","url":null,"abstract":"There was inefficient light absorption in the thin active layers due to optical losses in Organic Solar Cells (OSCs) with relatively large area. Therefore, it is a key issue to have a light trapping structure for highly efficient OSCs. For high performance devices fabrication, a smart\u0000 grating was fabricated using holographic photolithography incorporated with wet etching technology. Scanning electron microscopy (SEM) images of fabrication were employed before/after spin-coating active layer. With the aid of optical finite difference time Domain (FDTD) simulation for optical\u0000 effect, the optimized device structure ITO (1D grating)/PEDOT:PSS (40 nm)/PBDB-T:ITIC (100 nm)/PDINO (5 nm)/Al (100 nm) was obtained. The experimental results showed that when the grating period was 350 nm, depth 40 nm, the power conversion efficiencies (PCE) reached to 9.51%, an apparent\u0000 increase from those of the typical P3HT:PC71BM structure. This work indicates that the diffraction gratings had a potential to realize more efficient organic photovoltaics, if suitable fabrication processing methods can be developed.","PeriodicalId":18871,"journal":{"name":"Nanoscience and Nanotechnology Letters","volume":"12 1","pages":"484-489"},"PeriodicalIF":0.0,"publicationDate":"2020-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42128412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lemeng Chao, Shi Huanhuan, Kaixuan Nie, Bo Dong, Jiafeng Ding, Mengqiu Long, Zhengchun Liu
With the progress of micro-nano technology, the integration of microfluidic technology with a field effect transistor (FET) sensor has made portable biosensing devices of miniaturized structure available. As compared to traditional biosensors that requires large equipment and anti-interfering detection, FET biosensors integrated in microfluidic chips are fully-closed devices with the advantages of high sensitivity and accurate target capturing. Meanwhile FET biosensors integrated in microfluidic chips can be prepared by a simple, batch-produced manufacturing process to achieve label-free electrical detection. Herein, the progress of the FET biosensors integrated in microfluidic chips is reviewed in terms of sensing principle, configuration, and performance. Especially, the applications of these integrated biosensors in the areas of cell detection, gene detection, biomacromolecule detection, ion detection and pH detection are highlighted. This review provides a certain guiding role in the design and development of FET-based biosensors.
{"title":"Applications of Field Effect Transistor Biosensors Integrated in Microfluidic Chips","authors":"Lemeng Chao, Shi Huanhuan, Kaixuan Nie, Bo Dong, Jiafeng Ding, Mengqiu Long, Zhengchun Liu","doi":"10.1166/nnl.2020.3138","DOIUrl":"https://doi.org/10.1166/nnl.2020.3138","url":null,"abstract":"With the progress of micro-nano technology, the integration of microfluidic technology with a field effect transistor (FET) sensor has made portable biosensing devices of miniaturized structure available. As compared to traditional biosensors that requires large equipment and anti-interfering\u0000 detection, FET biosensors integrated in microfluidic chips are fully-closed devices with the advantages of high sensitivity and accurate target capturing. Meanwhile FET biosensors integrated in microfluidic chips can be prepared by a simple, batch-produced manufacturing process to achieve\u0000 label-free electrical detection. Herein, the progress of the FET biosensors integrated in microfluidic chips is reviewed in terms of sensing principle, configuration, and performance. Especially, the applications of these integrated biosensors in the areas of cell detection, gene detection,\u0000 biomacromolecule detection, ion detection and pH detection are highlighted. This review provides a certain guiding role in the design and development of FET-based biosensors.","PeriodicalId":18871,"journal":{"name":"Nanoscience and Nanotechnology Letters","volume":"12 1","pages":"427-445"},"PeriodicalIF":0.0,"publicationDate":"2020-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45491732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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