Pub Date : 2025-08-01Epub Date: 2025-07-23DOI: 10.1016/j.nano.2025.102846
Moumita Sil , Anamitra Goswami , Dipro Mukherjee , Nabanita Mukherjee , Igor Polikarpov , Arunava Goswami , Moupriya Nag , Dibyajit Lahiri
Biogenic silver nanoparticles (Bio-AgNPs) were synthesized using Withania somnifera root extract and evaluated for their antimicrobial and biosafety profiles. LC-MS confirmed key withanolides, including Withaferin A. The nanoparticles exhibited a UV–Vis peak at 446 nm, a crystalline core size of ∼15.65 nm, a hydrodynamic diameter of ∼134.85 nm, and a zeta potential of −25.29 mV. Bio-AgNPs showed potent antibacterial activity against Escherichia coli K12, with MICs between 156.25 and 312.5 ppm and superior efficacy to AgNO₃. RNA-seq analysis revealed downregulation of flagellar genes (flgD, flgG, fliC), maltose transporters (malK, malF, lamB), and citrate metabolism (citT, citD), indicating impaired motility and nutrient uptake. Upregulation of metal efflux (cusF, cusB) and electron transport (fixA, fixB) genes reflected oxidative stress response. Cytotoxicity assays on WI-38 and HEK-293 cells showed >80 % viability up to 156.25 ppm. These findings support Bio-AgNPs as biocompatible, plant-derived antimicrobials effective against Gram-negative bacteria.
{"title":"Withaferin A and Withanolide A-mediated silver nanoparticles target bacterial motility, metabolism, and stress adaptation in E. coli","authors":"Moumita Sil , Anamitra Goswami , Dipro Mukherjee , Nabanita Mukherjee , Igor Polikarpov , Arunava Goswami , Moupriya Nag , Dibyajit Lahiri","doi":"10.1016/j.nano.2025.102846","DOIUrl":"10.1016/j.nano.2025.102846","url":null,"abstract":"<div><div>Biogenic silver nanoparticles (Bio-AgNPs) were synthesized using <em>Withania somnifera</em> root extract and evaluated for their antimicrobial and biosafety profiles. LC-MS confirmed key withanolides, including Withaferin A. The nanoparticles exhibited a UV–Vis peak at 446 nm, a crystalline core size of ∼15.65 nm, a hydrodynamic diameter of ∼134.85 nm, and a zeta potential of −25.29 mV. Bio-AgNPs showed potent antibacterial activity against <em>Escherichia coli</em> K12, with MICs between 156.25 and 312.5 ppm and superior efficacy to AgNO₃. RNA-seq analysis revealed downregulation of flagellar genes (<em>flgD</em>, <em>flgG</em>, <em>fliC</em>), maltose transporters (<em>malK</em>, <em>malF</em>, <em>lamB</em>), and citrate metabolism (<em>citT</em>, <em>citD</em>), indicating impaired motility and nutrient uptake. Upregulation of metal efflux (<em>cusF</em>, <em>cusB</em>) and electron transport (<em>fixA</em>, <em>fixB</em>) genes reflected oxidative stress response. Cytotoxicity assays on WI-38 and HEK-293 cells showed >80 % viability up to 156.25 ppm. These findings support Bio-AgNPs as biocompatible, plant-derived antimicrobials effective against Gram-negative bacteria.</div></div>","PeriodicalId":19050,"journal":{"name":"Nanomedicine : nanotechnology, biology, and medicine","volume":"68 ","pages":"Article 102846"},"PeriodicalIF":4.2,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144696586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-08-01Epub Date: 2025-07-23DOI: 10.1016/j.nano.2025.102848
Xin Bao , Guang Xin , Qilong Zhou , Tao Wang , Xinrui Xu , Lijuan Feng , Chengyu Wan , Fangyuan Jing , Yijiang Liu , Yangbo Liu , Xiaoting Chen , Wen Huang
Acute pancreatitis (AP) is a common inflammatory disease that seriously threatens people's lives and health. Excessive reactive oxygen species (ROS) and cell-free DNA (cfDNA) are significant contributors to the advancement of AP. In this study, a nanoparticle with a dual function to clear ROS and cfDNA was developed for the treatment of acute pancreatitis. Cationic polyethyleneimine (PEI) was used to modify Mn3O4, resulting in the creation of Mn3O4-PEI nanoparticles, which exhibited strong binding to cfDNA and the ability to scavenge ROS. In vitro, Mn3O4-PEI showed significant inhibition of ROS and cfDNA-induced inflammation and demonstrated therapeutic effects in a model of acute pancreatitis induced by sodium taurocholate (STC). This research introduces a new nanomedicine approach for the treatment of AP.
{"title":"Dual-scavenging Mn3O4-PEI nanoparticles targeting ROS and cfDNA for acute pancreatitis therapy","authors":"Xin Bao , Guang Xin , Qilong Zhou , Tao Wang , Xinrui Xu , Lijuan Feng , Chengyu Wan , Fangyuan Jing , Yijiang Liu , Yangbo Liu , Xiaoting Chen , Wen Huang","doi":"10.1016/j.nano.2025.102848","DOIUrl":"10.1016/j.nano.2025.102848","url":null,"abstract":"<div><div>Acute pancreatitis (AP) is a common inflammatory disease that seriously threatens people's lives and health. Excessive reactive oxygen species (ROS) and cell-free DNA (cfDNA) are significant contributors to the advancement of AP. In this study, a nanoparticle with a dual function to clear ROS and cfDNA was developed for the treatment of acute pancreatitis. Cationic polyethyleneimine (PEI) was used to modify Mn<sub>3</sub>O<sub>4</sub>, resulting in the creation of Mn<sub>3</sub>O<sub>4</sub>-PEI nanoparticles, which exhibited strong binding to cfDNA and the ability to scavenge ROS. In vitro, Mn<sub>3</sub>O<sub>4</sub>-PEI showed significant inhibition of ROS and cfDNA-induced inflammation and demonstrated therapeutic effects in a model of acute pancreatitis induced by sodium taurocholate (STC). This research introduces a new nanomedicine approach for the treatment of AP.</div></div>","PeriodicalId":19050,"journal":{"name":"Nanomedicine : nanotechnology, biology, and medicine","volume":"68 ","pages":"Article 102848"},"PeriodicalIF":4.6,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144718222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-08-01Epub Date: 2025-06-30DOI: 10.1016/j.nano.2025.102838
Xingyue Wang , Kaige Qu , Rui Wang , Zuobin Wang , Baohua Jia
The conduction of electrical signal plays critical roles in neuron activities. The capacity of atomic force microscopy to establish physical contacts at the nanoscale and offer higher spatial resolution compared to conventional electrical recording methods, such as patch clamp, significantly enhances our understanding and analysis capabilities. This capability enables the direct visualization and precise quantification of the electrical properties within specific cellular membrane regions. In this study, we use conductive probes in our self-developed dual-probe atomic force microscope system as nanoelectrodes to measure the electrical signals of SH-SY5Y cell. Given that SH-SY5Y cells exhibit neuron-like properties and are relevant to human neurological diseases, researching these cells provides valuable insights into neuronal behavior and pathology. In dual-probe atomic force microscope, one probe is responsible for sending stimulation signals to the cells, while the other probe receives transmitted information from the cell. By modulating the pressure applied by the tip to the cell membrane, the effects on SH-SY5Y cells at different contact depths and time intervals were investigated, along with the monitoring of cellular potential changes. The changes in cell potential were detected. Two different nonlinear electrical characteristics were observed, which indicate the cell membrane exhibits adaptability and self-repair ability, achieved by regulating the state of ion channels to ensure membrane potential stability. These results provide a new way to stimulate and study the electrical characteristics and physiological behaviors of cells and other biological samples, potentially revealing new insights of neuron activities.
{"title":"Measurement of cell electrical signal by dual-probe atomic force microscopy","authors":"Xingyue Wang , Kaige Qu , Rui Wang , Zuobin Wang , Baohua Jia","doi":"10.1016/j.nano.2025.102838","DOIUrl":"10.1016/j.nano.2025.102838","url":null,"abstract":"<div><div>The conduction of electrical signal plays critical roles in neuron activities. The capacity of atomic force microscopy to establish physical contacts at the nanoscale and offer higher spatial resolution compared to conventional electrical recording methods, such as patch clamp, significantly enhances our understanding and analysis capabilities. This capability enables the direct visualization and precise quantification of the electrical properties within specific cellular membrane regions. In this study, we use conductive probes in our self-developed dual-probe atomic force microscope system as nanoelectrodes to measure the electrical signals of SH-SY5Y cell. Given that SH-SY5Y cells exhibit neuron-like properties and are relevant to human neurological diseases, researching these cells provides valuable insights into neuronal behavior and pathology. In dual-probe atomic force microscope, one probe is responsible for sending stimulation signals to the cells, while the other probe receives transmitted information from the cell. By modulating the pressure applied by the tip to the cell membrane, the effects on SH-SY5Y cells at different contact depths and time intervals were investigated, along with the monitoring of cellular potential changes. The changes in cell potential were detected. Two different nonlinear electrical characteristics were observed, which indicate the cell membrane exhibits adaptability and self-repair ability, achieved by regulating the state of ion channels to ensure membrane potential stability. These results provide a new way to stimulate and study the electrical characteristics and physiological behaviors of cells and other biological samples, potentially revealing new insights of neuron activities.</div></div>","PeriodicalId":19050,"journal":{"name":"Nanomedicine : nanotechnology, biology, and medicine","volume":"68 ","pages":"Article 102838"},"PeriodicalIF":4.2,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144554007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
We had previously examined the use of radioactive technetium-99m (Tc-99m)-labelled lactoferrin nanoparticles (LF-NPs) for sentinel lymph node (SLN) mapping. Compared with commercial tracer based on human serum albumin, LF-NPs exhibited better size uniformity and reduced batch variation. However, while in vitro tests with 99mTc-LF-NPs in RAW 264.7 macrophages were promising, the in vivo results revealed poor accumulation in the SLNs. To improve their in vivo performance, we performed surface modification of LF-NPs with different agents, including polyethylene glycol (PEG). Characterisation studies revealed that PEGylated LF-NPs (LF-NP@PEG) were the most effective. In vitro tests revealed significantly greater cellular uptake of LF-NP@PEG than unmodified LF-NPs. In vivo evaluation in animal model demonstrated increased SLN uptake and retention, which was supported by scintigraphic imaging studies. Although further refinements are needed, these findings suggest potential application for modified LF-NPs in SLN detection.
{"title":"Surface-modified 99mTc-Lactoferrin nanoparticles as tracers for sentinel lymph node mapping","authors":"Sanjay Kulkarni PhD , Anuj Kumar MSc , Soji Soman PhD , Abhijeet Pandey PhD , Suresh Subramanian PhD , Srinivas Mutalik PhD","doi":"10.1016/j.nano.2025.102835","DOIUrl":"10.1016/j.nano.2025.102835","url":null,"abstract":"<div><div>We had previously examined the use of radioactive technetium-99m (Tc-99m)-labelled lactoferrin nanoparticles (LF-NPs) for sentinel lymph node (SLN) mapping. Compared with commercial tracer based on human serum albumin, LF-NPs exhibited better size uniformity and reduced batch variation. However, while in vitro tests with <sup>99m</sup>Tc-LF-NPs in RAW 264.7 macrophages were promising, the in vivo results revealed poor accumulation in the SLNs. To improve their in vivo performance, we performed surface modification of LF-NPs with different agents, including polyethylene glycol (PEG). Characterisation studies revealed that PEGylated LF-NPs (LF-NP@PEG) were the most effective. In vitro tests revealed significantly greater cellular uptake of LF-NP@PEG than unmodified LF-NPs. In vivo evaluation in animal model demonstrated increased SLN uptake and retention, which was supported by scintigraphic imaging studies. Although further refinements are needed, these findings suggest potential application for modified LF-NPs in SLN detection.</div></div>","PeriodicalId":19050,"journal":{"name":"Nanomedicine : nanotechnology, biology, and medicine","volume":"68 ","pages":"Article 102835"},"PeriodicalIF":4.2,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144285770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-08-01Epub Date: 2025-06-16DOI: 10.1016/j.nano.2025.102837
Mahdiyar Dehshiri MSc , Fateme Zarein MSc , Fatemeh Rajabi MSc , Mohammad Reza Javan PhD , Maryam Nikkhah PhD , Fatemeh Rahbarizadeh PhD , Jalil Mehrzad PhD, DVM , Seyed Mohammad Moazzeni PhD , Amir Ali Hamidieh MD , Saman Hosseinkhani PhD
Genetic modification of immune cells remains a major challenge in immunotherapy. While viral and non-viral carriers exist, low gene transfer efficiency persists with non-viral methods. We present a peptide-based carrier (MiRGD) for gene delivery to diverse immune cells. The MiRGD/plasmid complex formation was characterized via gel retardation, dynamic light scattering, and zeta potential analysis. After safety and efficiency optimization in HEK293T cells, MiRGD achieved 69 % chimeric antigen receptor (CAR) transfection in Jurkat T cells (>98 % viability) and 28 % in primary human T cells. Dendritic cells showed 61 % transfection with >85 % viability. In vivo, MiRGD functioned as a DNA vaccine against SARS-CoV-2, eliciting robust antibody titers, neutralization, and safe histopathology. These results demonstrate MiRGD's efficacy and biocompatibility for immune cell engineering (T cells, dendritic cells, macrophages) and vaccination, offering a cost-effective, non-toxic platform for immunotherapy applications.
{"title":"Efficient gene delivery to immune cells via a recombinant multifunctional chimeric peptide nanocarrier: Implications in immunotherapy","authors":"Mahdiyar Dehshiri MSc , Fateme Zarein MSc , Fatemeh Rajabi MSc , Mohammad Reza Javan PhD , Maryam Nikkhah PhD , Fatemeh Rahbarizadeh PhD , Jalil Mehrzad PhD, DVM , Seyed Mohammad Moazzeni PhD , Amir Ali Hamidieh MD , Saman Hosseinkhani PhD","doi":"10.1016/j.nano.2025.102837","DOIUrl":"10.1016/j.nano.2025.102837","url":null,"abstract":"<div><div>Genetic modification of immune cells remains a major challenge in immunotherapy. While viral and non-viral carriers exist, low gene transfer efficiency persists with non-viral methods. We present a peptide-based carrier (MiRGD) for gene delivery to diverse immune cells. The MiRGD/plasmid complex formation was characterized via gel retardation, dynamic light scattering, and zeta potential analysis. After safety and efficiency optimization in HEK293T cells, MiRGD achieved 69 % chimeric antigen receptor (CAR) transfection in Jurkat T cells (>98 % viability) and 28 % in primary human T cells. Dendritic cells showed 61 % transfection with >85 % viability. In vivo, MiRGD functioned as a DNA vaccine against SARS-CoV-2, eliciting robust antibody titers, neutralization, and safe histopathology. These results demonstrate MiRGD's efficacy and biocompatibility for immune cell engineering (T cells, dendritic cells, macrophages) and vaccination, offering a cost-effective, non-toxic platform for immunotherapy applications.</div></div>","PeriodicalId":19050,"journal":{"name":"Nanomedicine : nanotechnology, biology, and medicine","volume":"68 ","pages":"Article 102837"},"PeriodicalIF":4.2,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144322453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-07-01Epub Date: 2025-05-03DOI: 10.1016/j.nano.2025.102827
Bongsu Jung Ph.D , Fazal-Ur-Rehman Bhatti Ph.D , Harisankeerth Mummareddy M.S , Youngjoo Kim M.A , Sang-Hyug Park Ph.D , Hongsik Cho Ph.D, MBA
Inflammation plays a significant role in the pathogenesis of knee post-traumatic osteoarthritis (PTOA) characterized by damage to cartilage and surrounding tissues that results in loss of physiological function. This inflammation is mainly regulated by NF-κB pathway. The TPCA-1 can inhibit IκB kinase (IKK) β in NF-κB pathway. Here, we optimized the delivery of TPCA-1 to the damaged knee joint via targeted nanosomes and examined its effects in a mouse model of PTOA.
PTOA was induced in mice through a modified cyclic mechanical loading method. Mice were divided into groups receiving vehicle, TPCA-1 solution, or TPCA-1-loaded nanosomes. A concentration of 100 μM TPCA-1 was used based on preliminary studies. Control groups included untreated and vehicle-treated animals. Treatment efficacy was assessed using in vivo imaging, serum biochemical assays, gene expression analysis of cartilage tissues, histopathology, and behavioral analysis.
Mechanical loading induced significant knee joint damage in the model. TPCA-1 nanosomes notably attenuated the adverse effects of loading, outperforming both the vehicle and TPCA-1-solution in reducing inflammation. Notably, serum levels of total NO and LDH were significantly lower in the TPCA-1-nanosome group. Inflammation, as indicated by MMP13 and IL1β gene expression, was substantially reduced. Enhanced cartilage preservation and function were confirmed through IVIS imaging, histological assessments, and improved behavior metrics.
The targeted delivery of TPCA-1 via nanosomes effectively inhibits the NF-κB pathway, leading to significant reductions in inflammation and cartilage damage in a PTOA mouse model. This strategy demonstrates potential as a therapeutic intervention for managing inflammation and preserving joint health in osteoarthritis.
{"title":"Targeted nanosome delivery of TPCA-1 for modulating inflammation in a mouse model of post-traumatic osteoarthritis","authors":"Bongsu Jung Ph.D , Fazal-Ur-Rehman Bhatti Ph.D , Harisankeerth Mummareddy M.S , Youngjoo Kim M.A , Sang-Hyug Park Ph.D , Hongsik Cho Ph.D, MBA","doi":"10.1016/j.nano.2025.102827","DOIUrl":"10.1016/j.nano.2025.102827","url":null,"abstract":"<div><div>Inflammation plays a significant role in the pathogenesis of knee post-traumatic osteoarthritis (PTOA) characterized by damage to cartilage and surrounding tissues that results in loss of physiological function. This inflammation is mainly regulated by NF-κB pathway. The TPCA-1 can inhibit IκB kinase (IKK) β in NF-κB pathway. Here, we optimized the delivery of TPCA-1 to the damaged knee joint via targeted nanosomes and examined its effects in a mouse model of PTOA.</div><div>PTOA was induced in mice through a modified cyclic mechanical loading method. Mice were divided into groups receiving vehicle, TPCA-1 solution, or TPCA-1-loaded nanosomes. A concentration of 100 μM TPCA-1 was used based on preliminary studies. Control groups included untreated and vehicle-treated animals. Treatment efficacy was assessed using in vivo imaging, serum biochemical assays, gene expression analysis of cartilage tissues, histopathology, and behavioral analysis.</div><div>Mechanical loading induced significant knee joint damage in the model. TPCA-1 nanosomes notably attenuated the adverse effects of loading, outperforming both the vehicle and TPCA-1-solution in reducing inflammation. Notably, serum levels of total NO and LDH were significantly lower in the TPCA-1-nanosome group. Inflammation, as indicated by MMP13 and IL1β gene expression, was substantially reduced. Enhanced cartilage preservation and function were confirmed through IVIS imaging, histological assessments, and improved behavior metrics.</div><div>The targeted delivery of TPCA-1 via nanosomes effectively inhibits the NF-κB pathway, leading to significant reductions in inflammation and cartilage damage in a PTOA mouse model. This strategy demonstrates potential as a therapeutic intervention for managing inflammation and preserving joint health in osteoarthritis.</div></div>","PeriodicalId":19050,"journal":{"name":"Nanomedicine : nanotechnology, biology, and medicine","volume":"67 ","pages":"Article 102827"},"PeriodicalIF":4.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143924791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-07-01Epub Date: 2025-05-11DOI: 10.1016/j.nano.2025.102828
Kamil Szeliski PhD , Zuzanna Fekner MSc , Damian Kasiński MSc , Marta Rasmus MSc , Filip Kowalski MD, PhD , Milena Świtońska Assoc. Prof. , Katarzyna Sierakowska Assoc. Prof. , Tomasz Drewa Prof. , Marta Pokrywczyńska Prof.
Diagnosing prostate cancer (PCa) and risk-stratifying patients remains challenging, as PSA-based methods lack precision for active surveillance (AS) decision-making. Extracellular vesicles (EVs) are membranous nano-sized vesicles released by all types of cells and may contain potentially interesting material for diagnostic procedures for PCa.
This study analyzed surface markers and miRNA profiles of medium-sized plasma EVs (mEVs) from 24 PCa patients using nanoflow cytometry and miRNA profiling. The ratio of PSMA+ EVs to PSMA+CD9+ EVs differed significantly between AS and non-AS patients. Additionally, miR-99a-5p, miR-125b-5p, miR-145-5p, and miR-365a-3p levels were higher in non-AS patients.
These findings suggest that plasma-derived PSMA+ mEVs originate from the prostate and may serve as biomarkers for PCa progression. Nanoflow cytometry-based analysis of EV surface markers combined with miRNA profiling provides a novel, non-invasive alternative to PSA measurements. This approach could improve risk stratification and decision-making for AS patients, potentially leading to better outcomes.
{"title":"The potential of plasma-derived medium-sized extracellular vesicles as a biopsy alternative for active surveillance decisions in prostate Cancer","authors":"Kamil Szeliski PhD , Zuzanna Fekner MSc , Damian Kasiński MSc , Marta Rasmus MSc , Filip Kowalski MD, PhD , Milena Świtońska Assoc. Prof. , Katarzyna Sierakowska Assoc. Prof. , Tomasz Drewa Prof. , Marta Pokrywczyńska Prof.","doi":"10.1016/j.nano.2025.102828","DOIUrl":"10.1016/j.nano.2025.102828","url":null,"abstract":"<div><div>Diagnosing prostate cancer (PCa) and risk-stratifying patients remains challenging, as PSA-based methods lack precision for active surveillance (AS) decision-making. Extracellular vesicles (EVs) are membranous nano-sized vesicles released by all types of cells and may contain potentially interesting material for diagnostic procedures for PCa.</div><div>This study analyzed surface markers and miRNA profiles of medium-sized plasma EVs (mEVs) from 24 PCa patients using nanoflow cytometry and miRNA profiling. The ratio of PSMA+ EVs to PSMA+CD9+ EVs differed significantly between AS and non-AS patients. Additionally, miR-99a-5p, miR-125b-5p, miR-145-5p, and miR-365a-3p levels were higher in non-AS patients.</div><div>These findings suggest that plasma-derived PSMA+ mEVs originate from the prostate and may serve as biomarkers for PCa progression. Nanoflow cytometry-based analysis of EV surface markers combined with miRNA profiling provides a novel, non-invasive alternative to PSA measurements. This approach could improve risk stratification and decision-making for AS patients, potentially leading to better outcomes.</div></div>","PeriodicalId":19050,"journal":{"name":"Nanomedicine : nanotechnology, biology, and medicine","volume":"67 ","pages":"Article 102828"},"PeriodicalIF":4.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144033178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-07-01Epub Date: 2025-05-22DOI: 10.1016/j.nano.2025.102829
Xiangning Luo BS , Renli Luo MS , Yuanyuan Zhou BS , Yuanpeng Jiang BS , Cong Han BS , Aiguo Song PhD , Kun Qian PhD , Chunrong Qu PhD , Rui Cao PhD , Bin Xu PhD , Zhen Cheng PhD
Gastrin-releasing peptide receptor (GRPR) is overexpressed in most prostate cancers (PCa) and is a potential target in diagnosis and treatment. In this study, based on the previously reported GRPR antagonist RM26 and novel chelating agent Dar derivatives, we designed and evaluated two radiopharmaceuticals, [68Ga]Ga-Dar-C5-P2-RM26 and [68Ga]Ga-Dar-P2-RM26. Both radiotracers were easily prepared at room temperature and showed high radiochemical stability in phosphate-buffered saline (PBS) and fetal bovine serum (FBS). Cellular and animal experiments indicated that the two radiotracers exhibited specific tumor uptakes in PC-3 xenograft mice models. Specifically, [68Ga]Ga-Dar-C5-P2-RM26 and [68Ga]Ga-Dar-P2-RM26 displayed 6.617 ± 0.245 % ID/g and 5.973 ± 1.261 % ID/g tumor uptake, respectively. Positron emission tomography/ computer tomography (PET/CT) imaging results indicated that these two radiotracers showed excellent tumor-to-background contrast at 0.5 h, 1 h, and 2 h post intravenous injection (p.i.). In summary, [68Ga]Ga-Dar-C5-RM26 and [68Ga]Ga-Dar-RM26 are GRPR-targeted radiotracers with high potential for clinical translation in tumor-targeted imaging.
{"title":"Design and synthesis of GRPR-targeted PET probes based on Dar derivatives for imaging of prostate cancer","authors":"Xiangning Luo BS , Renli Luo MS , Yuanyuan Zhou BS , Yuanpeng Jiang BS , Cong Han BS , Aiguo Song PhD , Kun Qian PhD , Chunrong Qu PhD , Rui Cao PhD , Bin Xu PhD , Zhen Cheng PhD","doi":"10.1016/j.nano.2025.102829","DOIUrl":"10.1016/j.nano.2025.102829","url":null,"abstract":"<div><div>Gastrin-releasing peptide receptor (GRPR) is overexpressed in most prostate cancers (PCa) and is a potential target in diagnosis and treatment. In this study, based on the previously reported GRPR antagonist RM26 and novel chelating agent Dar derivatives, we designed and evaluated two radiopharmaceuticals, [<sup>68</sup>Ga]Ga-Dar-C5-P2-RM26 and [<sup>68</sup>Ga]Ga-Dar-P2-RM26. Both radiotracers were easily prepared at room temperature and showed high radiochemical stability in phosphate-buffered saline (PBS) and fetal bovine serum (FBS). Cellular and animal experiments indicated that the two radiotracers exhibited specific tumor uptakes in PC-3 xenograft mice models. Specifically, [<sup>68</sup>Ga]Ga-Dar-C5-P2-RM26 and [<sup>68</sup>Ga]Ga-Dar-P2-RM26 displayed 6.617 ± 0.245 % ID/g and 5.973 ± 1.261 % ID/g tumor uptake, respectively. Positron emission tomography/ computer tomography (PET/CT) imaging results indicated that these two radiotracers showed excellent tumor-to-background contrast at 0.5 h, 1 h, and 2 h post intravenous injection (p.i.). In summary, [<sup>68</sup>Ga]Ga-Dar-C5-RM26 and [<sup>68</sup>Ga]Ga-Dar-RM26 are GRPR-targeted radiotracers with high potential for clinical translation in tumor-targeted imaging.</div></div>","PeriodicalId":19050,"journal":{"name":"Nanomedicine : nanotechnology, biology, and medicine","volume":"67 ","pages":"Article 102829"},"PeriodicalIF":4.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144134453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-07-01Epub Date: 2025-06-05DOI: 10.1016/j.nano.2025.102833
Man Li PhD, Yuting Yang PhD, Jiaojie Wei Master of Science, Xingli Cun PhD, Zhengze Lu PhD, Yue Qiu PhD, Zhirong Zhang PhD, Qin He PhD
{"title":"Corrigendum to “enhanced chemo-immunotherapy against melanoma by inhibition of cholesterol esterification in CD8+ T cells” [nanomedicine: nanotechnology, biology, and medicine 14 (2018) 2541–2550]","authors":"Man Li PhD, Yuting Yang PhD, Jiaojie Wei Master of Science, Xingli Cun PhD, Zhengze Lu PhD, Yue Qiu PhD, Zhirong Zhang PhD, Qin He PhD","doi":"10.1016/j.nano.2025.102833","DOIUrl":"10.1016/j.nano.2025.102833","url":null,"abstract":"","PeriodicalId":19050,"journal":{"name":"Nanomedicine : nanotechnology, biology, and medicine","volume":"67 ","pages":"Article 102833"},"PeriodicalIF":4.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144221376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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