T. Harada, M. Hiruma, K. Nishimoto, Taku Kato, A. Nishikawa, T. Mochizuki, M. Fujihiro
{"title":"The 50th Anniversary Educational Symposium of the Japanese Society for Medical Mycolgy","authors":"T. Harada, M. Hiruma, K. Nishimoto, Taku Kato, A. Nishikawa, T. Mochizuki, M. Fujihiro","doi":"10.3314/JJMM.48.115","DOIUrl":"https://doi.org/10.3314/JJMM.48.115","url":null,"abstract":"","PeriodicalId":19301,"journal":{"name":"Nippon Ishinkin Gakkai Zasshi","volume":"16 1","pages":"115-136"},"PeriodicalIF":0.0,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74110380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E. Muroi, Y. Hamasaki, K. Nishimoto, S. Mohri, H. Sagara, Emiko Tanabe, N. Kameda, N. Suzuki, S. Tejima, A. Kurashima, A. Hebisawa
A 53-year-old man was admitted to Keio University Hospital because of serious dyspnea and edema of the lower extremities. Eighteen months previously, the patient had complained of chest discomfort, and was then admitted for the first time to our hospital for evaluation of chest pain. Electrocardiography showed poor R wave progression in leads Vl through V4, and diffuse nonspecific STsegment and T wave abnormalities with low voltage. However, no definitive diagnosis could be made at this initial admission and a calcium-channel blocker was prescribed. Despite this treatment, the patient was readmitted with worsening dyspnea and lower extremity edema. The diagnosis of heart failure and nephritic syndrome was made at the second admission. In addition, immunoelectrophoresis showed a monoclonal IgD (l) M protein and increased plasma cells in the bone marrow, suggesting a diagnosis of multiple myeloma. The patient was thus given dexamethasone (20 mg per day for 4 days) intravenously, but his symptoms did not improve. Two weeks later, the patient deteriorated further with congestive heart failure and renal failure, and subsequently died of cardiac arrest with ventricular fibrillation. On autopsy, IgD (l)-positive plasma cell proliferation was found in the bone marrow, confirming the diagnosis of multiple myeloma. In addition, amyloid deposition was detected in various organs including the heart, kidneys, esophagus, duodenum, ileum, colon, tongue, and lungs. In particular, the weight of the heart was 650 g demonstrating a hypertrophic septum and amyloid deposition in the myocardium and even coronary arteries. In summary, the final diagnosis was IgD (l) multiple myeloma associated with systemic amyloidosis. (Keio J Med 53 (3): 178–191, September 2004)
{"title":"Clinico-Pathological Conference","authors":"E. Muroi, Y. Hamasaki, K. Nishimoto, S. Mohri, H. Sagara, Emiko Tanabe, N. Kameda, N. Suzuki, S. Tejima, A. Kurashima, A. Hebisawa","doi":"10.3314/JJMM.44.307","DOIUrl":"https://doi.org/10.3314/JJMM.44.307","url":null,"abstract":"A 53-year-old man was admitted to Keio University Hospital because of serious dyspnea and edema of the lower extremities. Eighteen months previously, the patient had complained of chest discomfort, and was then admitted for the first time to our hospital for evaluation of chest pain. Electrocardiography showed poor R wave progression in leads Vl through V4, and diffuse nonspecific STsegment and T wave abnormalities with low voltage. However, no definitive diagnosis could be made at this initial admission and a calcium-channel blocker was prescribed. Despite this treatment, the patient was readmitted with worsening dyspnea and lower extremity edema. The diagnosis of heart failure and nephritic syndrome was made at the second admission. In addition, immunoelectrophoresis showed a monoclonal IgD (l) M protein and increased plasma cells in the bone marrow, suggesting a diagnosis of multiple myeloma. The patient was thus given dexamethasone (20 mg per day for 4 days) intravenously, but his symptoms did not improve. Two weeks later, the patient deteriorated further with congestive heart failure and renal failure, and subsequently died of cardiac arrest with ventricular fibrillation. On autopsy, IgD (l)-positive plasma cell proliferation was found in the bone marrow, confirming the diagnosis of multiple myeloma. In addition, amyloid deposition was detected in various organs including the heart, kidneys, esophagus, duodenum, ileum, colon, tongue, and lungs. In particular, the weight of the heart was 650 g demonstrating a hypertrophic septum and amyloid deposition in the myocardium and even coronary arteries. In summary, the final diagnosis was IgD (l) multiple myeloma associated with systemic amyloidosis. (Keio J Med 53 (3): 178–191, September 2004)","PeriodicalId":19301,"journal":{"name":"Nippon Ishinkin Gakkai Zasshi","volume":"78 1","pages":"307-319"},"PeriodicalIF":0.0,"publicationDate":"2003-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87088842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
K. Asano, T. Tamura, H. Iizuka, H. Kubo, H. Shibaki
{"title":"Dermatophyte Flora at the Department of Dermatology of Asahikawa Medical College During a Period of 1990 to 1995","authors":"K. Asano, T. Tamura, H. Iizuka, H. Kubo, H. Shibaki","doi":"10.3314/JJMM.38.303","DOIUrl":"https://doi.org/10.3314/JJMM.38.303","url":null,"abstract":"","PeriodicalId":19301,"journal":{"name":"Nippon Ishinkin Gakkai Zasshi","volume":"38 1","pages":"303-310"},"PeriodicalIF":0.0,"publicationDate":"1997-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87043376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Genome Information and the Repetitive Sequences in Candida albicans","authors":"Y. Nakagawa","doi":"10.3314/JJMM.38.279","DOIUrl":"https://doi.org/10.3314/JJMM.38.279","url":null,"abstract":"Candida albicansで頻繁に観察される染色体サイズの多型現象の研究が発端となって,反復配列RPSが発見された.RPSはゲノム内に60-80コピー存在し,いくつかの染色体では複数のRPSが直列に配置している.多数のRPSをゲノムDNAよりクローン化し,塩基配列を決定したところ,多くの興味ある事実が得られた.RPSには172bpからなる内部反復配列altが存在し,この配列の繰り返しの多様性がRPSのサイズ,塩基配列の多様性を生み出していることが明らかになった.RPSの解析を進める過程で,RPSに隣接した領域が,RPS同様に各染色体に存在していることがわかってきた.ノーザン分析の結果より,この領域がRNAに転写されていることがわかった.そこで,cDNAライブラリよりクローン化して配列決定を行ったところ,機能すると予想されるORFが存在しなかったことから,この領域がRNA分子として働いていることが想像された.最近になって,RPSに隣接する新規反復配列を発見し,これらの反復配列群がまとまって染色体の構成や機能に必須の役割を果たしていると考えている.C.albicansをめぐる以上のようなゲノム,遺伝子に関する情報は,インターネット上に蓄積され,研究者が共有できる情報としてますます充実しつつある.","PeriodicalId":19301,"journal":{"name":"Nippon Ishinkin Gakkai Zasshi","volume":"55 1","pages":"279-283"},"PeriodicalIF":0.0,"publicationDate":"1997-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89482205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
細胞や組織の特異性を明らかにする方法として,発現しているmRNAの差異を解析することは,きわめて有用なアプローチである.しかし,従来のサブトラクション法に代表される解析法は,試料調製や手技的にさまざまな問題点があった.PCR法を応用してmRNA発現量の違いを解析する方法(Differential Display, DDやmRNA fingerprinting using arbitrarily primed PCR; RAP)は,その有用性のみならず,比較的簡便に行えることから,さまざまな領域で応用されている.本稿では,DD法の原理といくつかの問題点について解説するとともに,C.albicansの感染性因子の解析を目的として,感染力の異なるC.albicans株間で行った我々の実験例を紹介する.
作为明确细胞和组织特异性的方法，分析所发现的mRNA的差异是非常有用的方法。但是，以传统的子牵引法为代表的分析法在样品制备和技术上各种各样。应用PCR法分析mRNA表达量差异的方法(Differential Display，DD或mRNA fingerprinting using arbitrarily primed PCR;RAP)不仅因其实用性，而且因其操作简便，被广泛应用于各个领域。本文将对DD法的原理和几个问题进行解说，同时介绍我们在具有不同感染力的C.albicans株之间进行的实验，目的是解析C.albicans的感染性因子。
{"title":"Molecular Cloning of Candida albicans Genes by Differential Display","authors":"S. Nakashima, Y. Kitajima, Y. Nozawa, F. Mirbod","doi":"10.3314/JJMM.38.291","DOIUrl":"https://doi.org/10.3314/JJMM.38.291","url":null,"abstract":"細胞や組織の特異性を明らかにする方法として,発現しているmRNAの差異を解析することは,きわめて有用なアプローチである.しかし,従来のサブトラクション法に代表される解析法は,試料調製や手技的にさまざまな問題点があった.PCR法を応用してmRNA発現量の違いを解析する方法(Differential Display, DDやmRNA fingerprinting using arbitrarily primed PCR; RAP)は,その有用性のみならず,比較的簡便に行えることから,さまざまな領域で応用されている.本稿では,DD法の原理といくつかの問題点について解説するとともに,C.albicansの感染性因子の解析を目的として,感染力の異なるC.albicans株間で行った我々の実験例を紹介する.","PeriodicalId":19301,"journal":{"name":"Nippon Ishinkin Gakkai Zasshi","volume":"38 1","pages":"291-295"},"PeriodicalIF":0.0,"publicationDate":"1997-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86898120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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