{"title":"Taxonomical Outline of Dermatophytes in the Ascomycetes","authors":"S. Udagawa","doi":"10.3314/JJMM.38.1","DOIUrl":"https://doi.org/10.3314/JJMM.38.1","url":null,"abstract":"不整子嚢菌類ホネタケ目はAspergillus,Penicilliumのテレオモルフであるユーロチウム目と多くの点で対比される菌群である.本目には多数の皮膚糸状菌が含まれ,長らく医学的に関心が寄せられてきた.ホネタケ目には次の4科がある.アルスロデルマ科は子嚢果壁の特徴,子嚢胞子の形態,Chrysosporium, Microsporum, Trichophytonに属するアレウロ型分生子の形成などに基づく均質な菌群である.ギムノアスクス科は明色,菌糸のゆるい網目構造からなる子嚢果を形成し,円盤形,やや不規則な壁の子嚢胞子を内生する.ケラチン分解性とセルロース分解性の菌があり,分節分生子を形成する種がある.ミクソトリクム科は暗色の子嚢果で,しばしば樹枝状の付属物を形成する.子嚢果壁は厚壁の菌糸が網目状になり,子嚢胞子は紡錘形,通常縦に筋がある.Malbranchea,Oidiodendron属の分節型分生子がみられ,セルロース分解性を示す.ホネタケ科の属は非常に多様性であり,とくに子嚢果壁に変化がみられる.Aphanoascus,Arachnomycesは膜質の閉子嚢殻を形成する.Amauroascus,Nannizziopsisは単純な菌糸の網目構造である.一方,Onygenaの子嚢果は柄があって,その先端部で菌糸が広がり球形の頭部になる高度に特殊化された形態となる.本科は通常凹点のある子嚢胞子とケラチン分解性を特徴としている.","PeriodicalId":19301,"journal":{"name":"Nippon Ishinkin Gakkai Zasshi","volume":"2 1","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"1997-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88928383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
G. You, Jing Wang, Bing-mei Liu, Song Yu, Lie Ma, De-yuang You, Xue-zhu Jin, Y. Bai
A survey of dermatophytes isolated from patients was made from 1980 to 1996 in Northeast China. We analyzed 2031 strains of dermatophytes, which are positive in culture in 3 provinces (Heilongjiang, Jilin, Liaoning): 742 strains were obtained from the China-Japan Friendship Hospital of Heilongjiang (1980-1993), 846 strains from the 2nd Hospital of Bethuene Medical University (1986-1989), and 443 strains from the People's Hospital of Liaoning Province (1989-1996).The result of the survey indicated that the main etiologic fungus of dermatophytosis in Northeast China was T. rubrum 1163 strains (57.26%), T. mentagrophytes 402 (19.79%), M. canis 296 (14.57%), E. floccosum 100 (4.92%), T. verrucosum 28 (1.38%), M. ferrugineum 10 (0.49%), M. gypseum 10 (0.49%), T. schoenleinii 4 (0.20%), T. tonsurans 3 (0.15%), and the root 15 strains were Candida albicans (4) Aspergillus (7), Penicillum (2) and Alternaria (2).The first 3 species are basically the same as those in Beijing, Tianjing, and Taiyuan. M. canis is rarely seen in Huanan, Huazhong, and Qinghai. However, T. verrucosum is notably influenced by geographical factors. Kerion celsi and sycosis trichophytica are caused by T. verrucosum (43.75%) in Heilongjiang. T. verrucosum is hardy and can live in cold areas. It once exploded simultaneously in human and animals in Hellongjiang, Xinjiang, and NingXia China.
{"title":"Geographical Distribution of Species on Etiologic Fungi of Dermatophytosis in Northeast China","authors":"G. You, Jing Wang, Bing-mei Liu, Song Yu, Lie Ma, De-yuang You, Xue-zhu Jin, Y. Bai","doi":"10.3314/JJMM.38.9","DOIUrl":"https://doi.org/10.3314/JJMM.38.9","url":null,"abstract":"A survey of dermatophytes isolated from patients was made from 1980 to 1996 in Northeast China. We analyzed 2031 strains of dermatophytes, which are positive in culture in 3 provinces (Heilongjiang, Jilin, Liaoning): 742 strains were obtained from the China-Japan Friendship Hospital of Heilongjiang (1980-1993), 846 strains from the 2nd Hospital of Bethuene Medical University (1986-1989), and 443 strains from the People's Hospital of Liaoning Province (1989-1996).The result of the survey indicated that the main etiologic fungus of dermatophytosis in Northeast China was T. rubrum 1163 strains (57.26%), T. mentagrophytes 402 (19.79%), M. canis 296 (14.57%), E. floccosum 100 (4.92%), T. verrucosum 28 (1.38%), M. ferrugineum 10 (0.49%), M. gypseum 10 (0.49%), T. schoenleinii 4 (0.20%), T. tonsurans 3 (0.15%), and the root 15 strains were Candida albicans (4) Aspergillus (7), Penicillum (2) and Alternaria (2).The first 3 species are basically the same as those in Beijing, Tianjing, and Taiyuan. M. canis is rarely seen in Huanan, Huazhong, and Qinghai. However, T. verrucosum is notably influenced by geographical factors. Kerion celsi and sycosis trichophytica are caused by T. verrucosum (43.75%) in Heilongjiang. T. verrucosum is hardy and can live in cold areas. It once exploded simultaneously in human and animals in Hellongjiang, Xinjiang, and NingXia China.","PeriodicalId":19301,"journal":{"name":"Nippon Ishinkin Gakkai Zasshi","volume":"1 1","pages":"9-12"},"PeriodicalIF":0.0,"publicationDate":"1997-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89415172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kazuhiro Endo, T. Kashimura, Katsuhiko Yoshida, A. Handa, T. Sakata, Katsuro Ito, M. Fukuda, I. Jinnai, I. Murohashi, M. Bessho, M. Saito, K. Hirashima, Y. Inoue
症例は,19歳男性,1992年9月急性リンパ性白血病(FAB分類ALL L2)と診断しJapan Adult Leukemia study Group (JAL-SG)プロトコールにて治療し完全寛解に到った後,1994年8月,再発しprednisolone, vincristineによる寛解導入療法を行った.その後,cytarabine,l-asparaginaseにて治療し長期間,骨髄抑制が起こり,発熱と潰瘍底を形成し中心部が壊死に陥った皮疹が全身に認められるようになった.皮膚生検を行い,培養の結果Fusarium solaniを検出した.そのため,granulocyte colony-stimulating factor (G-CSF) 350μgを連日静注し,amphotericin B (AMPH)を点滴静注し1mgから開始し40mgまで増量した.しかし,原疾患の悪化に伴いPseudomonas aeruginosaによる敗血症を併発し死亡した.剖検では全身各種臓器にもFusariumによる播種性病変が認められた.とくに肺,消化管,腎,心,膵,筋肉に多発性病変を,また腎は一部梗塞を伴う病変を認めた.今後,免疫不全患者の増加によりこのような弱毒真菌感染症が増加するものと思われた.
病例为19岁男性,1992年9月诊断为急性淋巴细胞白血病(FAB分类ALL L2) Japan Adult Leukemia study Group(JAL-SG)协议治疗完全缓解后,1994年8月,复发prednisolone,使用vincristine的缓解导入疗法。之后使用cytarabine和l-asparaginase治疗,长时间出现骨髓抑制,全身出现发热和溃疡底部坏死的疹。变得能做了。进行皮肤活检,培养结果检测出Fusarium solani,因此granulocyte colony-stimulating factor (G-CSF)连续几天静注350μg,amphotericin B (AMPH)从1mg开始增加到40mg。但是,随着原病的恶化,假单胞菌aeruginosa并发败血症而死亡。剖检发现全身各种脏器均有Fusarium引起的播种性病变,特别是肺、消化道、肾、心、胰、肌肉出现多发性病变,肾出现部分梗塞病变。今后,由于免疫缺陷患者的增加,这种弱毒真菌感染症将会增加。
{"title":"A Case of Systemic Fusarium solani Infection Complicating Acute Lymphocytic Leukemia","authors":"Kazuhiro Endo, T. Kashimura, Katsuhiko Yoshida, A. Handa, T. Sakata, Katsuro Ito, M. Fukuda, I. Jinnai, I. Murohashi, M. Bessho, M. Saito, K. Hirashima, Y. Inoue","doi":"10.3314/JJMM.38.81","DOIUrl":"https://doi.org/10.3314/JJMM.38.81","url":null,"abstract":"症例は,19歳男性,1992年9月急性リンパ性白血病(FAB分類ALL L2)と診断しJapan Adult Leukemia study Group (JAL-SG)プロトコールにて治療し完全寛解に到った後,1994年8月,再発しprednisolone, vincristineによる寛解導入療法を行った.その後,cytarabine,l-asparaginaseにて治療し長期間,骨髄抑制が起こり,発熱と潰瘍底を形成し中心部が壊死に陥った皮疹が全身に認められるようになった.皮膚生検を行い,培養の結果Fusarium solaniを検出した.そのため,granulocyte colony-stimulating factor (G-CSF) 350μgを連日静注し,amphotericin B (AMPH)を点滴静注し1mgから開始し40mgまで増量した.しかし,原疾患の悪化に伴いPseudomonas aeruginosaによる敗血症を併発し死亡した.剖検では全身各種臓器にもFusariumによる播種性病変が認められた.とくに肺,消化管,腎,心,膵,筋肉に多発性病変を,また腎は一部梗塞を伴う病変を認めた.今後,免疫不全患者の増加によりこのような弱毒真菌感染症が増加するものと思われた.","PeriodicalId":19301,"journal":{"name":"Nippon Ishinkin Gakkai Zasshi","volume":"9 1","pages":"81-85"},"PeriodicalIF":0.0,"publicationDate":"1997-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74278797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Toshihiko Watanabe, T. Mikami, Tatsuji Matsumoto, Masuko Suzuki
Meth-A fibrosarcoma-implanted mice were examined for protective activity to lethal Candida albicans infection. The number of peripheral blood polymorphonuclear leukocytes (PMNs) was markedly increased in the mice, and their candidacidal function was also activated. The growth of C. albicans invitro was inhibited by the addition of serum from Meth-A-implanted mice. This activity was inhibitedby the addition of an inhibitor of transferrin, ferric sulphate. These findings indicated that C. albicans cells were efficiently eliminated by the activated PMNs and that the elimination was increased by the serum transferrin in Meth-A-implanted mice. leukocytes, Transferrin,
{"title":"Anticandidal Activity of Polymorphonuclear Leukocytes and Serum Transferrin in Tumor-Implanted Mice","authors":"Toshihiko Watanabe, T. Mikami, Tatsuji Matsumoto, Masuko Suzuki","doi":"10.3314/JJMM.38.61","DOIUrl":"https://doi.org/10.3314/JJMM.38.61","url":null,"abstract":"Meth-A fibrosarcoma-implanted mice were examined for protective activity to lethal Candida albicans infection. The number of peripheral blood polymorphonuclear leukocytes (PMNs) was markedly increased in the mice, and their candidacidal function was also activated. The growth of C. albicans invitro was inhibited by the addition of serum from Meth-A-implanted mice. This activity was inhibitedby the addition of an inhibitor of transferrin, ferric sulphate. These findings indicated that C. albicans cells were efficiently eliminated by the activated PMNs and that the elimination was increased by the serum transferrin in Meth-A-implanted mice. leukocytes, Transferrin,","PeriodicalId":19301,"journal":{"name":"Nippon Ishinkin Gakkai Zasshi","volume":"19 1","pages":"61-65"},"PeriodicalIF":0.0,"publicationDate":"1997-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81841938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Molecular Biological Approach to Phylogeny and Identification of Dermatophytes","authors":"K. Makimura, Hideaki Goto, T. Mochizuki, A. Hasegawa, K. Uchida, H. Yamaguchi","doi":"10.3314/JJMM.38.5","DOIUrl":"https://doi.org/10.3314/JJMM.38.5","url":null,"abstract":"菌種特異的DNA塩基配列の解析に基づいた皮膚糸状菌の系統解析法および同定法に関して概説した.このような「塩基配列レベル」の解析によって,皮膚糸状菌を含む広汎な病原真菌の系統分類に有用な新たな指標が与えられるのみではなく,菌体DNAからの主要病原真菌の迅速同定も可能となり,真菌症の診断法,治療法,および予防法の進歩に大きく貢献するものと期待できる.","PeriodicalId":19301,"journal":{"name":"Nippon Ishinkin Gakkai Zasshi","volume":"113 1","pages":"5-8"},"PeriodicalIF":0.0,"publicationDate":"1997-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78706381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Y. Hasegawa, T. Nikai, Y. Okumura, K. Ogawa, H. Sugihara
An elastolytic proteinase was isolated from Aspergillus flavus by column chromatography using diethylaminoethyl (DEAE)-cellulose and carboxymethyl (CM)-Sephadex C-50. The proteinase was found to be homogeneous as indicated by a single band after disc polyacrylamide gel electrophoresis (PAGE). The enzyme had a molecular weight of 40, 000Da as determined by sodium dodecyl sulfate (SDS)-PAGE.The elastolytic activity was inhibited by leupeptin, diisopropyl fluorophosphate (DFP), α1-antitrypsin, α2-macroglobulin and ethylenediaminetetraacetic acid (EDTA). However, neither N-bromo-succinimide (NBS) nor antithrombin-III showed any inhibitory effect on the enzyme activity. The enzyme contained 414 amino acid residues and exhibited an isoelectric point of 8.6. Its carbohydrate content was calculated to be 3.6% using glucose as a standard, but elastolytic proteinase from A. fumigatus did not contain any carbohydrates. The Aα, Bβ and γ chains of human fibrinogen were cleaved by the enzyme.Elastolytic proteinase from A. flavus hydrolyzed Ser(9)-His(10), Val(12)-Glu(13), Glu(13)-Ala(14), Ala(14)-Leu(15), Leu(15)-Tyr(16), Tyr(16)-Leu(17), Glu(21)-Arg(22), Phe(25)-Tyr(26), Tyr(26)-Thr(27), Pro(28)-Lys(29) and Lys(29)-Ala(30) bonds of oxidized insulin B chain, showing that enzyme has proteolytic activity.However, elastolytic proteinase from A. fumigatus had a molecular weight of 32, 000Da, and the enzyme did not contain carbohydrate.
{"title":"Characterization of Elastolytic Proteinase from Aspergillus flavus:Comparison of Biochemical Properties to the Elastolytic Proteinase from Aspergillus fumigatus","authors":"Y. Hasegawa, T. Nikai, Y. Okumura, K. Ogawa, H. Sugihara","doi":"10.3314/JJMM.38.53","DOIUrl":"https://doi.org/10.3314/JJMM.38.53","url":null,"abstract":"An elastolytic proteinase was isolated from Aspergillus flavus by column chromatography using diethylaminoethyl (DEAE)-cellulose and carboxymethyl (CM)-Sephadex C-50. The proteinase was found to be homogeneous as indicated by a single band after disc polyacrylamide gel electrophoresis (PAGE). The enzyme had a molecular weight of 40, 000Da as determined by sodium dodecyl sulfate (SDS)-PAGE.The elastolytic activity was inhibited by leupeptin, diisopropyl fluorophosphate (DFP), α1-antitrypsin, α2-macroglobulin and ethylenediaminetetraacetic acid (EDTA). However, neither N-bromo-succinimide (NBS) nor antithrombin-III showed any inhibitory effect on the enzyme activity. The enzyme contained 414 amino acid residues and exhibited an isoelectric point of 8.6. Its carbohydrate content was calculated to be 3.6% using glucose as a standard, but elastolytic proteinase from A. fumigatus did not contain any carbohydrates. The Aα, Bβ and γ chains of human fibrinogen were cleaved by the enzyme.Elastolytic proteinase from A. flavus hydrolyzed Ser(9)-His(10), Val(12)-Glu(13), Glu(13)-Ala(14), Ala(14)-Leu(15), Leu(15)-Tyr(16), Tyr(16)-Leu(17), Glu(21)-Arg(22), Phe(25)-Tyr(26), Tyr(26)-Thr(27), Pro(28)-Lys(29) and Lys(29)-Ala(30) bonds of oxidized insulin B chain, showing that enzyme has proteolytic activity.However, elastolytic proteinase from A. fumigatus had a molecular weight of 32, 000Da, and the enzyme did not contain carbohydrate.","PeriodicalId":19301,"journal":{"name":"Nippon Ishinkin Gakkai Zasshi","volume":"9 1","pages":"53-59"},"PeriodicalIF":0.0,"publicationDate":"1997-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75131354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}