PharmaNutrition最新文献_第10页

Assessment of dietary habits and use of nutritional supplements in COVID-19: A cross-sectional study 评估COVID-19患者的饮食习惯和营养补充剂的使用:一项横断面研究

IF 3.2 Q3 NUTRITION & DIETETICS

PharmaNutrition

Pub Date : 2022-12-01 DOI: 10.1016/j.phanu.2022.100309

Cagla Ayer , Adviye Gulcin Sagdicoglu Celep

Objective

Determine nutritional status and use of food supplements during COVID-19.

Methods

Sample of individuals aged 18–65 participated in study voluntarily. Patients with COVID-19 or individuals with contact were not included. Questionnaire form was prepared based on literature on food supplements and included questions adapted to COVID-19 pandemic.

Results

488 participants completed the study. Participants’ mean age was 26.28 ± 7.64, %82.0 were female, 88.3% were high educated. During COVID-19, 33.6% exercise less than 150 min/day, 55.1% evaluate their eating habits as good/very good, and number of meals did not change compared to before pandemic (41.8%). 34.2% of participants consume 5–7 cups of water daily during pandemic. Consumption of pastry (54.7%) and green leafy vegetables (49.6%) increased. 78.7% of participants did not use any nutritional supplement, and 51.1% stated that they did not need nutritional supplements. Multivitamin and mineral (16.5%), vitamin D (15.3%), and vitamin C (11.4%) are used the most, and 56.7% have been using these products for 1–3 months. Use of nutritional supplements was recommended mostly by doctors (39.4%). 43.1% stated that they used these products to maintain good health, 21.9% because they felt tired, 13.8% because they did not have adequate and balanced nutrition. 51.0% of those using nutritional supplements benefited, 30.8% had no effect.

Conclusions

Participants did not make significant changes in their eating habits compared to before pandemic, the use of nutritional supplements increased to maintain good health.

目的确定COVID-19期间的营养状况和食品补充剂的使用情况。方法选取年龄在18 ~ 65岁之间的个人自愿参与研究。未包括COVID-19患者或与其接触的个人。根据有关食品补充剂的文献编制了问卷表，其中包括适应COVID-19大流行的问题。结果488名参与者完成了研究。参与者平均年龄26.28±7.64岁，女性占82.0 %，高学历占88.3%。在COVID-19期间，33.6%的人每天运动不到150分钟，55.1%的人认为他们的饮食习惯很好/非常好，与大流行前相比，用餐次数没有变化(41.8%)。34.2%的参与者在大流行期间每天饮用5-7杯水。糕点(54.7%)和绿叶蔬菜(49.6%)的消费量有所增加。78.7%的参与者没有使用任何营养补充剂，51.1%的参与者表示他们不需要营养补充剂。使用最多的是复合维生素和矿物质(16.5%)、维生素D(15.3%)、维生素C(11.4%)， 56.7%的人使用了1-3个月。使用营养补充剂主要是由医生推荐的(39.4%)。43.1%的人说他们使用这些产品是为了保持身体健康，21.9%的人说他们感到疲劳，13.8%的人说他们没有得到足够和均衡的营养。使用营养补充剂的人中有51.0%的人受益，30.8%的人没有效果。结论与大流行前相比，参与者的饮食习惯没有明显改变，营养补充剂的使用增加了，以保持良好的健康。

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引用次数: 4

Functional differences between primary monocyte-derived and THP-1 macrophages and their response to LCPUFAs 原代单核细胞源性和THP-1巨噬细胞的功能差异及其对LCPUFAs的反应

IF 3.2 Q3 NUTRITION & DIETETICS

PharmaNutrition

Pub Date : 2022-12-01 DOI: 10.1016/j.phanu.2022.100322

Tamara Hoppenbrouwers , Shanna Bastiaan-Net , Johan Garssen , Nicoletta Pellegrini , Linette E.M. Willemsen , Harry J. Wichers

Background

In immune cell models, macrophages are one of the most frequently used cell types. THP-1 cells are often used as model to study macrophage function, however they may act differently from primary human monocyte derived macrophages (MDMs).

Methods

In this study, we investigated the intrinsic baseline differences between the human macrophage cell line THP-1 and human primary MDMs. Additionally, we studied the difference in response to treatment with long-chain polyunsaturated fatty acids (LCPUFAs): well-described immunomodulators.

Results

Although the amount of cells that phagocytose were similar between the cell types, primary MDMs consumed significantly more E. coli bioparticles compared to THP-1 macrophages. In M1 macrophages, IL-12 secretion was almost fifty times higher by primary MDMs compared to THP-1 macrophages, thereby increasing the IL-12/IL-10 ratio. Despite this, the IL-12 secretion by THP-1 M1 macrophages was higher that the secretion of IL-10, thereby showing that it is still a suitable M1 type. Cytokine profiles differed between primary MDMs and THP-1 M1 and M2 macrophages. In response to LCPUFAs, primary M1 MDMs and THP-1 M1 macrophages were alike. Interestingly, primary M2 MDMs secreted less IL-10 and CCL22 when treated with LCPUFAs, whereas THP-1 M2 macrophages secreted more IL-10 when treated with LCPUFAs and showed no difference in CCL22 secretion.

Conclusions

In conclusion, in an M1 setting, both THP-1 and primary MDMs are suitable models. However, when interested in M2 models, the model choice highly depends on the research question.

在免疫细胞模型中，巨噬细胞是最常用的细胞类型之一。THP-1细胞经常被用作研究巨噬细胞功能的模型，然而它们的行为可能与原代人单核细胞来源的巨噬细胞(MDMs)不同。方法研究人巨噬细胞系THP-1与人原代MDMs的内在基线差异。此外，我们研究了长链多不饱和脂肪酸(LCPUFAs)治疗反应的差异，LCPUFAs是一种描述良好的免疫调节剂。结果虽然不同细胞类型的吞噬细胞数量相似，但与THP-1巨噬细胞相比，原代MDMs消耗的大肠杆菌生物颗粒明显更多。在M1巨噬细胞中，原代MDMs分泌的IL-12几乎是THP-1巨噬细胞的50倍，从而增加了IL-12/IL-10比值。尽管如此，THP-1 M1巨噬细胞分泌IL-12高于分泌IL-10，这表明它仍然是一种合适的M1型。细胞因子谱在原代MDMs和THP-1 M1和M2巨噬细胞之间存在差异。对LCPUFAs的反应，原代M1 MDMs和THP-1 M1巨噬细胞相似。有趣的是，原代M2巨噬细胞在LCPUFAs处理下分泌较少的IL-10和CCL22，而THP-1 M2巨噬细胞在LCPUFAs处理下分泌更多的IL-10，且CCL22的分泌没有差异。结论在M1环境下，THP-1和原发性MDMs都是合适的模型。然而，当对M2模型感兴趣时，模型的选择高度依赖于研究问题。

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引用次数: 2

Vitamin D status can affect COVID-19 outcomes also in pediatric population 维生素D状况也会影响儿科人群的COVID-19结局

IF 3.2 Q3 NUTRITION & DIETETICS

PharmaNutrition

Pub Date : 2022-12-01 DOI: 10.1016/j.phanu.2022.100319

Michele Miraglia del Giudice , Cristiana Indolfi , Giulio Dinardo , Fabio Decimo , Alberto Decimo , Angela Klain

Background

vitamin D influences the immune system and the inflammatory response. It is known that vitamin D supplementation reduces the risk of acute respiratory tract infection. In the last two years, many researchers have investigated vitamin D’s role in the pathophysiology of COVID-19 disease.

Results

the findings obtained from clinical trials and systematic reviews highlight that most patients with COVID-19 have decreased vitamin D levels and low levels of vitamin D increase the risk of severe disease. This evidence seems to be also confirmed in the pediatric population.

Conclusions

further studies (systematic review and meta-analysis) conducted on children are needed to confirm that vitamin D affects COVID-19 outcomes and to determine the effectiveness of supplementation and the appropriate dose, duration and mode of administration.

维生素D影响免疫系统和炎症反应。众所周知，补充维生素D可以降低急性呼吸道感染的风险。在过去的两年中，许多研究人员研究了维生素D在COVID-19疾病病理生理学中的作用。结果临床试验和系统评价的结果强调，大多数COVID-19患者维生素D水平降低，维生素D水平低会增加严重疾病的风险。这一证据似乎在儿科人群中也得到了证实。结论:需要对儿童进行进一步的研究(系统评价和荟萃分析)，以证实维生素D会影响COVID-19的结局，并确定补充维生素D的有效性以及适当的剂量、持续时间和给药方式。

引用次数: 7

Inulin prebiotic dietary supplementation improves metabolic parameters by reducing the Toll-like receptor 4 transmembrane protein gene and interleukin 6 expression in adipose tissue 饲粮中添加菊粉可通过降低脂肪组织中toll样受体4跨膜蛋白基因和白细胞介素6的表达来改善代谢参数

IF 3.2 Q3 NUTRITION & DIETETICS

PharmaNutrition

Pub Date : 2022-12-01 DOI: 10.1016/j.phanu.2022.100316

Carla Dayana Durães Abreu , Bruna Viana Caldas , Guilherme Henrique Mendes Ribeiro , Charles Martins Aguilar , Lucyana Conceição Farias , André Luiz Sena Guimarães , Alfredo Maurício Batista de Paula , Maria Beatriz Abreu Glória , Sérgio Henrique Sousa Santos

Background

Lifestyle modifications have increased the consumption of ultra-processed foods, fat, sugar, and salt worldwide and also decreased dietary fiber due to reduced legume, grain, and vegetable intake. Ultimately, the contemporary food intake profile has increased the incidence of obesity and chronic inflammatory diseases. Probiotic dietary supplementation is a viable health option.

Methods

Male Swiss mice were fed with two diets for four weeks: standard and standard + Inulin. The serum biochemistry data, histology, and inflammatory-related genes’ mRNA expression in adipose tissue were analyzed.

Results

The primary results showed that inulin dietary supplementation decreased the Toll-like receptor 4 transmembrane protein gene (TLR4) expression and interleukin 6 (IL6) in the epididymal adipose tissue, resulting in decreased adipocyte hypertrophy. The high-density lipoprotein (HDL) was increased and TGP enzymes (related to liver injury risk) were decreased also improving insulin sensitivity.

Conclusion

The present study revealed that inulin supplementation could improve the onset of chronic inflammation-related markers in adipose tissue indicating that inulin is a natural anti-inflammatory alternative.

Data Availability Statement

The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.

生活方式的改变增加了世界范围内超加工食品、脂肪、糖和盐的消费量，同时由于豆类、谷物和蔬菜摄入量的减少，膳食纤维也减少了。最终，当代食物摄入状况增加了肥胖和慢性炎症疾病的发病率。益生菌膳食补充剂是一种可行的健康选择。方法采用标准饲粮和标准饲粮+菊粉两种饲粮喂养4周。分析血清生化、组织学及脂肪组织炎症相关基因mRNA表达情况。结果饲粮中添加菊粉可降低附睾脂肪组织中toll样受体4跨膜蛋白基因(TLR4)和白细胞介素6 (IL6)的表达，导致脂肪细胞肥厚减少。高密度脂蛋白(HDL)升高，TGP酶(与肝损伤风险相关)降低，并改善胰岛素敏感性。结论补充菊粉可以改善脂肪组织中慢性炎症相关标志物的发生，提示菊粉是一种天然的抗炎替代品。数据可用性声明当前研究期间生成和/或分析的数据集可根据通讯作者的合理要求提供。

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引用次数: 2

Green coffee extract (Coffea canephora) improved the intestinal barrier and slowed colorectal cancer progression and its associated inflammation in rats 绿咖啡提取物(Coffea canephora)改善了大鼠的肠道屏障，减缓了结直肠癌的进展及其相关的炎症

IF 3.2 Q3 NUTRITION & DIETETICS

PharmaNutrition

Pub Date : 2022-12-01 DOI: 10.1016/j.phanu.2022.100314

Caroline Woelffel Silva , Keila Rodrigues Zanardi , Mariana Grancieri , Neuza Maria Brunoro Costa , Leonardo Oliveira Trivillin , Mirelle Lomar Viana , Pollyanna Ibrahim Silva , André Gustavo Vasconcelos Costa

Background and aim

Cancer is directly associated with inflammation and oxidative stress. Thanks to its antioxidant activity, green coffee may have anticarcinogenic effects. The objective of this study was to assess the effect of an aqueous green coffee extract on local colorectal morphophysiological and systemic inflammatory and oxidative changes in an animal model of colorectal cancer (CRC).

Methods

CRC was induced in male Wistar rats for 5 weeks by dimethylhydrazine. After 10 weeks of carcinogenesis, the rats were divided: Healthy Control (HC, without cancer induction and without extract), Colorectal Cancer (CRC, with cancer induction and without extract), Health Green Coffee (HGC, without cancer induction and with extract) and colorectal cancer treatment with Green Coffee (CGC, with cancer induction and with extract) groups. The data were analyzed using the Student’s t-test or ANOVA and the Newman-Keuls post-hoc test (p < 0.05).

Results

Green coffee extract was source of caffeine, chlorogenic acid, and trigonelline that had in silico interaction with NF-κB. This extract contributed to the intestinal barrier integrity by decreasing lactulose and mannitol urinary excretion, increasing fecal IgA levels and reducing malignant tumors in the colon and rectum. In addition, it reduced the systemic production of the inflammatory cytokines TNFα and IL-6. However, green coffee extract had no effect on aberrant crypt foci (ACF) or colonic pH and no difference was found in oxidative changes.

Conclusions

Green coffee extract has intestinal benefits, through the action of its bioactive compounds in the microenvironment of the neoplastic lesion, thereby opening research avenues for new studies.

背景和目的癌症与炎症和氧化应激直接相关。由于其抗氧化活性，绿咖啡可能具有抗癌作用。本研究的目的是评估水溶绿咖啡提取物对结直肠癌(CRC)动物模型局部结肠形态生理和全身炎症和氧化变化的影响。方法用二甲肼诱导雄性Wistar大鼠scrc 5周。癌变10周后，将大鼠分为健康对照组(HC组，无癌诱导，无提取物组)、结直肠癌组(CRC组，有癌诱导，无提取物组)、健康绿咖啡组(HGC组，无癌诱导，有提取物组)和结直肠癌绿咖啡组(CGC组，有癌诱导，有提取物组)。使用学生t检验或方差分析和Newman-Keuls事后检验(p <0.05)。结果绿原酸和葫芦巴碱是与NF-κB相互作用的咖啡因、绿原酸和葫芦巴碱的主要来源。该提取物通过减少尿中乳果糖和甘露醇的排泄，增加粪便IgA水平，减少结肠和直肠的恶性肿瘤，有助于肠道屏障的完整性。此外，它还减少了全身炎症细胞因子TNFα和IL-6的产生。然而，绿咖啡提取物对异常隐窝灶(ACF)或结肠pH没有影响，氧化变化也没有差异。结论绿咖啡提取物通过其生物活性化合物在肿瘤病变微环境中的作用对肠道有益，从而为新的研究开辟了研究途径。

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引用次数: 0

The anti-proliferative effect of β-carotene against a triple-negative breast cancer cell line is cancer cell-specific and JNK-dependent β-胡萝卜素对三阴性乳腺癌细胞系的抗增殖作用是癌细胞特异性的和jnk依赖性的

IF 3.2 Q3 NUTRITION & DIETETICS

PharmaNutrition

Pub Date : 2022-12-01 DOI: 10.1016/j.phanu.2022.100320

Ana Antunes , Francisca Carmo , Sara Pinto , Nelson Andrade , Fátima Martel

Background

Breast cancer is a major cause of cancer-related mortality in women worldwide. Triple-negative breast cancer presents an aggressive behavior and a poor response to therapeutic. Cancer progression is associated with reprogramming of metabolic pathways for glutamine, glucose and folic acid.

Methods

In this study, we characterized the antitumoral effect (effects on cell proliferation, culture growth, viability, migration, oxidative stress levels, cell cycle and apoptosis) of carotenoids on a triple-negative human breast cancer cell line (MDA-MB-231 cell line) and investigated interference with nutrient cellular uptake as a contributing mechanism.

Results

Of the four tested carotenoids (β-carotene, crocin, fucoxanthin, astaxanthin), β-carotene presented the most interesting antitumoral effect, by reducing cell proliferation, migration, viability and culture growth, inducing apoptosis and by interfering with cell cycle (S phase arrest). β-carotene significantly increased ³H-deoxy-D-glucose uptake but did not affect neither ³H-glutamine nor ³H-folic acid uptake. Also, it did not interfere with oxidative stress levels. The anti-proliferative effect of β-carotene involves the JNK intracellular pathway, and this carotenoid was able to enhance the anti-proliferative effect of doxorubicin. Importantly, β-carotene did not affect cell viability, proliferation, cell cycle and migration rates of MCF-12A cells, a non-tumoral human breast epithelial cell line.

Conclusion

β-carotene presents potential as co-adjuvant to doxorubicin for triple-negative breast cancer treatment.

背景乳腺癌是世界范围内女性癌症相关死亡的主要原因。三阴性乳腺癌表现出侵略性行为和治疗反应差。癌症进展与谷氨酰胺、葡萄糖和叶酸代谢途径的重编程有关。方法研究了类胡萝卜素对三阴性人乳腺癌细胞系(MDA-MB-231)的抗肿瘤作用(对细胞增殖、培养生长、活力、迁移、氧化应激水平、细胞周期和凋亡的影响)，并探讨了干扰营养细胞摄取的机制。结果在4种类胡萝卜素(β-胡萝卜素、西红花素、岩藻黄素、虾青素)中，β-胡萝卜素通过抑制细胞增殖、迁移、存活和培养生长、诱导细胞凋亡和干扰细胞周期(S期阻滞)表现出最显著的抗肿瘤作用。β-胡萝卜素显著增加3h -脱氧-d -葡萄糖的摄取，但对3h -谷氨酰胺和3h -叶酸的摄取没有影响。此外，它不会干扰氧化应激水平。β-胡萝卜素的抗增殖作用涉及JNK细胞内通路，这种类胡萝卜素能够增强阿霉素的抗增殖作用。重要的是，β-胡萝卜素不影响MCF-12A细胞(一种非肿瘤人乳腺上皮细胞系)的细胞活力、增殖、细胞周期和迁移率。结论β-胡萝卜素可作为阿霉素辅助治疗三阴性乳腺癌的潜在药物。

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引用次数: 3

A specific combination of nutraceutical Ingredients exerts cytoprotective effects in human cholinergic neurons 营养成分的特定组合对人类胆碱能神经元具有细胞保护作用

IF 3.2 Q3 NUTRITION & DIETETICS

PharmaNutrition

Pub Date : 2022-12-01 DOI: 10.1016/j.phanu.2022.100317

Elisa Zappelli , Simona Daniele , Matteo Vergassola , Lorenzo Ceccarelli , Elisa Chelucci , Giorgina Mangano , Lucia Durando , Lorella Ragni , Claudia Martini

Background

Brain aging is associated with an excessive reactive oxygen species (ROS) formation that causes cell injury through proteins oxidation and DNA damage. These changes have been identified as contributing factors in age-related memory decline. In this sense, treatments able to protect central nervous system (CNS) from oxidative stress and to sustain membrane plasticity, may represent new candidates to counter the development of aging effects. Several studies have indicated vitamin E, folic acid, magnesium and omega-3 as nutraceuticals protecting CNS from oxidative stress.

Methods

A specific association of these active nutrients was tested in human cholinergic neurons, chosen as a cellular model related to learning and memory processes. Cortisol was used as an oxidative stress insult to explore the beneficial properties of the nutraceuticals.

Results

In summary, the specific ratio of active ingredients in the above selected food supplement prevented the decrease in ATP content and in cell viability exerted by cortisol. At the same time, it prevented ROS formation, DNA damage, autophagy processes and decrease in the expression of cellular well-being genes induced by cell treatment with cortisol. The effects on ATP content, ROS formation and cellular viability were evidenced when the nutraceutical mix when administered following cortisol treatment, too. Notably, these peculiar evidences were significantly higher with respect to those elicited by the single components of the food supplement.

Conclusions

Overall, these results confirm the beneficial effects of the simultaneous administration of vitamin E, folic acid, magnesium and omega-3.

脑老化与活性氧(ROS)的过量形成有关，活性氧通过蛋白质氧化和DNA损伤导致细胞损伤。这些变化已被确定为与年龄相关的记忆衰退的因素。从这个意义上说，能够保护中枢神经系统(CNS)免受氧化应激和维持膜可塑性的治疗可能代表了对抗衰老效应发展的新候选。几项研究表明，维生素E、叶酸、镁和omega-3是保护中枢神经系统免受氧化应激的营养保健品。方法在人类胆碱能神经元中检测这些活性营养素的特异性关联，选择胆碱能神经元作为学习和记忆过程相关的细胞模型。皮质醇被用作氧化应激损伤来探索营养保健品的有益特性。结果综上所述，上述食物补充剂中活性成分的特定比例可以防止皮质醇对ATP含量和细胞活力的降低。同时，它还能阻止ROS的形成、DNA损伤、自噬过程以及皮质醇诱导的细胞健康基因表达的减少。对ATP含量、ROS形成和细胞活力的影响也被证明是在皮质醇治疗后服用营养保健品混合物。值得注意的是，这些特殊的证据明显高于那些由食品补充剂的单一成分引起的证据。总之，这些结果证实了同时服用维生素E、叶酸、镁和omega-3的有益效果。

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引用次数: 1

Enhanced nutraceutical functions of herbal oily extract employing formulation technology: The present and future 利用配方技术增强草药油提取物的营养保健功能:现在与未来

IF 3.2 Q3 NUTRITION & DIETETICS

PharmaNutrition

Pub Date : 2022-12-01 DOI: 10.1016/j.phanu.2022.100318

Mizuki Ogino, Kohei Yamada, Hideyuki Sato, Satomi Onoue

Background

In spite of attractive nutraceutical functions, herbal products for oral use have many drawbacks, including poor oral-absorption and storage stability, leading to limited usability and efficacy. Considerable attention has been drawn to formulation studies on herbal materials for improvement of biopharmaceutical and nutraceutical properties. The aim of this review is to provide an illustrative overview of industrial and academic formulation researches on herbal materials for overcoming their problems.

Methods

The literature search was conducted for recent findings in the development of herbal formulations with enhanced nutraceutical effects.

Results

A number of formulations have been applied to herbal materials, includes emulsions, self-emulsifying drug delivery systems, liposomes, solid nanostructured particles, solid dispersions, and cyclodextrin-complexation. These formulation strategies can provide improved dissolution properties and oral absorption, a wide safety margin, better handling, and/or enhanced storage stability, possibly resulting in higher product values. Whereas nutraceutical properties have a higher priority, the selection of a suitable formulation would also be necessary for each herbal extract, considering manufacturability, safety concerns, and usability. Herbal medicines still include many unknowns for daily use, and evidence on some nutraceutical properties is insufficient; therefore, they should continue to be researched in more detail.

Conclusion

Strategic use of formulation approaches might provide a bright future for self-medication with herbal products.

尽管具有诱人的营养保健功能，但口服草药产品存在许多缺点，包括口服吸收和储存稳定性差，导致可用性和疗效有限。为了提高生物制药和营养保健性能，草药材料的配方研究已经引起了相当大的关注。本文旨在对中药制剂的工业和学术研究进行综述，以克服中药制剂存在的问题。方法对近年来在中药制剂开发方面的研究成果进行文献检索。结果许多制剂已应用于中草药，包括乳剂、自乳化给药系统、脂质体、固体纳米结构颗粒、固体分散体和环糊精络合物。这些配方策略可以提供更好的溶解性能和口服吸收，更宽的安全裕度，更好的处理，和/或增强的储存稳定性，可能导致更高的产品价值。虽然营养成分具有更高的优先级，但考虑到可制造性、安全性和可用性，每种草药提取物选择合适的配方也是必要的。草药的日常使用仍有许多未知之处，一些营养成分的证据不足;因此，他们应该继续进行更详细的研究。结论有策略地运用处方方法，为中药产品自我药疗提供了良好的前景。

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引用次数: 0

In vitro effects of vitamins C and E on adipocyte function and redox status in obesity 维生素C和E对肥胖患者脂肪细胞功能和氧化还原状态的体外影响

IF 3.2 Q3 NUTRITION & DIETETICS

PharmaNutrition

Pub Date : 2022-12-01 DOI: 10.1016/j.phanu.2022.100315

Asmahan Imessaoudene , Amel Z. Merzouk , Baya Guermouche , Hafida Merzouk , Sid Ahmed Merzouk

Background

Obesity is associated with a chronic inflammatory state and evident oxidative stress. Antioxidant supplementation may have beneficial effects on adipocyte function and on oxidative stress. Therefore, in this study, we investigated the in vitro effects of vitamins C, E on redox and metabolic parameters in adipocytes of control and obese Wistar rats.

Methods

Adipocytes were isolated from abdominal adipose tissue and were cultured in RPMI medium for 24 h, in the presence or the absence of vitamins (C, E at 50 µM). Glucose consumption, lactate and glycerol release, ATP and triglyceride contents, redox balance were investigated with biochemical methods.

Results

The results showed altered glucose consumption and lactate and glycerol efflux, high triglycerides, low ATP contents (P < 0.01), associated to an intracellular oxidative stress in adipocytes of obese rats. Vitamins C and E restored adipocyte function in obesity. Vitamin E increased lipolysis in adipocytes during obesity (P < 0.01). Redox balance was also modulated by antioxidants, including a reduction in intracellular hydroperoxides and carbonyl proteins levels and an increase in catalase and SOD activities and glutathione contents in adipocytes of obese rats.

Conclusion

Treatments with vitamins had beneficial effects on adipocyte function, and should be considered in therapeutic approaches for normalizing adipose function in obesity.

背景:肥胖与慢性炎症状态和明显的氧化应激有关。补充抗氧化剂可能对脂肪细胞功能和氧化应激有有益的影响。因此，在本研究中，我们研究了维生素C、E对正常和肥胖Wistar大鼠脂肪细胞氧化还原和代谢参数的体外影响。方法从腹部脂肪组织中分离脂肪细胞，在RPMI培养基中培养24 h，在50µM时分别添加或不添加维生素C、E。葡萄糖消耗、乳酸和甘油释放、ATP和甘油三酯含量、氧化还原平衡用生化方法测定。结果葡萄糖消耗、乳酸和甘油外排改变，甘油三酯高，ATP含量低(P <0.01)，与肥胖大鼠脂肪细胞内氧化应激有关。维生素C和E可以恢复肥胖患者的脂肪细胞功能。维生素E增加肥胖期间脂肪细胞的脂肪分解(P <0.01)。氧化还原平衡也被抗氧化剂调节，包括细胞内氢过氧化物和羰基蛋白水平的降低，肥胖大鼠脂肪细胞中过氧化氢酶和超氧化物歧化酶活性和谷胱甘肽含量的增加。结论维生素治疗对脂肪细胞功能有有益影响，应作为肥胖患者脂肪功能正常化的治疗手段之一。

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引用次数: 1

Pro-, prebiotics, and other healthful supplements taking the stage in Rome 益生菌，益生元和其他健康补品在罗马登台

IF 3.2 Q3 NUTRITION & DIETETICS

PharmaNutrition

Pub Date : 2022-12-01 DOI: 10.1016/j.phanu.2022.100321

Francesco Visioli , Lucio Capurso

引用次数: 0