Mingming Yang, Changhuan Du, Meng Li, Yuanzhuo Wang, Gege Bao, Jinxiu Huang, Qingyan Zhang, Shuzhen Zhang, Pengfei Xu, Weili Teng, Qingqing Li, Shanshan Liu, Bo Song, Qiang Yang, Zhikun Wang
Soybean [Glycine max (L.) Merr.] is a major oil-producing crop worldwide. Although several related proteins regulating soybean oil accumulation have been reported, little is known about the regulatory mechanisms. In this study, we characterized vascular plant one-zinc-finger 1A (GmVOZ1A) that interacts with WRINKLED 1a (GmWRI1a) using yeast two-hybrid library screening. The GmVOZ1A–GmWRI1a interaction was further verified by protein–protein interaction assays in vivo and in vitro. GmVOZ1A enhanced the seed fatty acid and oil contents by regulating genes involved in lipid biosynthesis. Conversely, a loss-of-function mutation in GmVOZ1A resulted in a reduction in triacylglycerol (TAG) content in soybean. Protein–DNA interaction assays revealed that GmVOZ1A and GmWRI1a cooperate to up-regulate the expression level of acyl-coenzymeA-binding protein 6a (GmACBP6a) and promote the accumulation of TAG. In addition, GmACBP6a overexpression promoted seed fatty acid and oil contents, as well as increased seed size and 100-seed weight. Taken together, these findings indicate that the transcription factor GmVOZ1A regulates soybean oil synthesis and cooperates with GmWRI1a to up-regulate GmACBP6a expression and oil biosynthesis in soybean. The results lay a foundation for a comprehensive understanding of the regulatory mechanisms underlying soybean oil biosynthesis and will contribute to improving soybean oil production through molecular breeding approaches.
{"title":"The transcription factors GmVOZ1A and GmWRI1a synergistically regulate oil biosynthesis in soybean","authors":"Mingming Yang, Changhuan Du, Meng Li, Yuanzhuo Wang, Gege Bao, Jinxiu Huang, Qingyan Zhang, Shuzhen Zhang, Pengfei Xu, Weili Teng, Qingqing Li, Shanshan Liu, Bo Song, Qiang Yang, Zhikun Wang","doi":"10.1093/plphys/kiae485","DOIUrl":"https://doi.org/10.1093/plphys/kiae485","url":null,"abstract":"Soybean [Glycine max (L.) Merr.] is a major oil-producing crop worldwide. Although several related proteins regulating soybean oil accumulation have been reported, little is known about the regulatory mechanisms. In this study, we characterized vascular plant one-zinc-finger 1A (GmVOZ1A) that interacts with WRINKLED 1a (GmWRI1a) using yeast two-hybrid library screening. The GmVOZ1A–GmWRI1a interaction was further verified by protein–protein interaction assays in vivo and in vitro. GmVOZ1A enhanced the seed fatty acid and oil contents by regulating genes involved in lipid biosynthesis. Conversely, a loss-of-function mutation in GmVOZ1A resulted in a reduction in triacylglycerol (TAG) content in soybean. Protein–DNA interaction assays revealed that GmVOZ1A and GmWRI1a cooperate to up-regulate the expression level of acyl-coenzymeA-binding protein 6a (GmACBP6a) and promote the accumulation of TAG. In addition, GmACBP6a overexpression promoted seed fatty acid and oil contents, as well as increased seed size and 100-seed weight. Taken together, these findings indicate that the transcription factor GmVOZ1A regulates soybean oil synthesis and cooperates with GmWRI1a to up-regulate GmACBP6a expression and oil biosynthesis in soybean. The results lay a foundation for a comprehensive understanding of the regulatory mechanisms underlying soybean oil biosynthesis and will contribute to improving soybean oil production through molecular breeding approaches.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":null,"pages":null},"PeriodicalIF":7.4,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142231558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Linking timing to nitrogen use efficiency: rice OsEC-Ghd7-ARE1 module works on it.","authors":"Munkhtsetseg Tsednee","doi":"10.1093/plphys/kiae488","DOIUrl":"https://doi.org/10.1093/plphys/kiae488","url":null,"abstract":"","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":null,"pages":null},"PeriodicalIF":7.4,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142233303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Guardians of the light: The redox regulation of the photosystem I during photosynthesis.","authors":"Sara Selma","doi":"10.1093/plphys/kiae482","DOIUrl":"https://doi.org/10.1093/plphys/kiae482","url":null,"abstract":"","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":null,"pages":null},"PeriodicalIF":7.4,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142233304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Taijoon Chung, Ye Eun Choi, Kyoungjun Song, Hyera Jung
Autophagy is a membrane trafficking pathway through which eukaryotic cells target their own cytoplasmic constituents for degradation in the lytic compartment. Proper biogenesis of autophagic organelles requires a conserved set of autophagy-related (ATG) proteins and their interacting factors, such as signalling phospholipid phosphatidylinositol 3-phosphate (PI3P) and coat complex II (COPII). The COPII machinery, which was originally identified as a membrane coat involved in the formation of vesicles budding from the endoplasmic reticulum, contributes to the initiation of autophagic membrane formation in yeast, metazoan, and plant cells; however, the exact mechanisms remain elusive. Recent studies using the plant model species Arabidopsis thaliana have revealed that plant-specific PI3P effectors are involved in autophagy. The PI3P effector FYVE2 interacts with the conserved PI3P effector ATG18 and with COPII components, indicating an additional role for the COPII machinery in the later stages of autophagosome biogenesis. In this Update, we examined recent research on plant autophagosome biogenesis and proposed working models on the functions of the COPII machinery in autophagy, including its potential roles in stabilizing membrane curvature and sealing the phagophore.
自噬是一种膜转运途径，真核细胞通过自噬将自身的细胞质成分在溶解区降解。自噬细胞器的正常生物生成需要一组保守的自噬相关（ATG）蛋白及其相互作用因子，如信号磷脂磷脂酰肌醇 3-磷酸（PI3P）和外皮复合体 II（COPII）。COPII 机制最初被认为是一种膜衣，参与了从内质网出芽的囊泡的形成，在酵母、元类动物和植物细胞中有助于自噬膜形成的启动；然而，其确切的机制仍然难以捉摸。最近利用植物模式物种拟南芥（Arabidopsis thaliana）进行的研究发现，植物特异性 PI3P 效应子参与了自噬。PI3P 效应子 FYVE2 与保守的 PI3P 效应子 ATG18 以及 COPII 成分相互作用，表明 COPII 机制在自噬体生物发生的后期阶段发挥了额外的作用。在《最新进展》中，我们考察了最近关于植物自噬体生物发生的研究，并就 COPII 机器在自噬中的功能提出了工作模型，包括其在稳定膜曲率和密封吞噬体方面的潜在作用。
{"title":"How coat proteins shape autophagy in plant cells","authors":"Taijoon Chung, Ye Eun Choi, Kyoungjun Song, Hyera Jung","doi":"10.1093/plphys/kiae426","DOIUrl":"https://doi.org/10.1093/plphys/kiae426","url":null,"abstract":"Autophagy is a membrane trafficking pathway through which eukaryotic cells target their own cytoplasmic constituents for degradation in the lytic compartment. Proper biogenesis of autophagic organelles requires a conserved set of autophagy-related (ATG) proteins and their interacting factors, such as signalling phospholipid phosphatidylinositol 3-phosphate (PI3P) and coat complex II (COPII). The COPII machinery, which was originally identified as a membrane coat involved in the formation of vesicles budding from the endoplasmic reticulum, contributes to the initiation of autophagic membrane formation in yeast, metazoan, and plant cells; however, the exact mechanisms remain elusive. Recent studies using the plant model species Arabidopsis thaliana have revealed that plant-specific PI3P effectors are involved in autophagy. The PI3P effector FYVE2 interacts with the conserved PI3P effector ATG18 and with COPII components, indicating an additional role for the COPII machinery in the later stages of autophagosome biogenesis. In this Update, we examined recent research on plant autophagosome biogenesis and proposed working models on the functions of the COPII machinery in autophagy, including its potential roles in stabilizing membrane curvature and sealing the phagophore.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":null,"pages":null},"PeriodicalIF":7.4,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142170932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jinbiao Wang, Qi Zheng, Ruizhong Zhang, Zhaoyu Huang, Qingyu Wu, Lei Liu, Qiang Ning, David Jackson, Fang Xu
Heterozygous mutations in two genes encoding key regulators of development improve kernel row number in inbred and hybrid maize, revealing their potential for yield improvement.
{"title":"Heterozygous fasciated ear mutations improve yield traits in inbred and hybrid maize lines","authors":"Jinbiao Wang, Qi Zheng, Ruizhong Zhang, Zhaoyu Huang, Qingyu Wu, Lei Liu, Qiang Ning, David Jackson, Fang Xu","doi":"10.1093/plphys/kiae472","DOIUrl":"https://doi.org/10.1093/plphys/kiae472","url":null,"abstract":"Heterozygous mutations in two genes encoding key regulators of development improve kernel row number in inbred and hybrid maize, revealing their potential for yield improvement.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":null,"pages":null},"PeriodicalIF":7.4,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142170931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yujun Hou, Darren C J Wong, Xiaoming Sun, Qingyun Li, Huimin Zhou, Lin Meng, Xiaoli Liao, Zhenchang Liang, Rishi Aryal, Qingfeng Wang, Haiping Xin
Cold stress is an adverse environmental factor that limits the growth and productivity of horticulture crops such as grapes (Vitis vinifera). In this study, we identified a grapevine cold-induced basic helix–loop–helix (bHLH) transcription factor (VvbHLH036). Overexpression and CRISPR/Cas9-mediated knockout (KO) of VvbHLH036 enhanced and decreased cold tolerance in grapevine roots, respectively. Transcriptome analysis of VvbHLH036-overexpressed roots identified threonine synthase (VvThrC1) as a potential downstream target of VvbHLH036. We confirmed that VvbHLH036 could bind the VvThrC1 promoter and activate its expression. Both the transcripts of VvThrC1 and the content of threonine were significantly induced in the leaves and roots of grapevine under cold treatment compared to controls. Conversely, these dynamics were significantly suppressed in the roots of CRISPR/Cas9-induced knockout of VvbHLH036. These observations support the regulation of threonine accumulation by VvbHLH036 through VvThrC1 during cold stress in grapevine. Furthermore, overexpression and CRISPR/Cas9-mediated knockout of VvThrC1 also confirmed its role in regulating threonine content and cold tolerance in transgenic roots at low temperature. Exogenous threonine treatment increased cold tolerance and reduced the accumulation of superoxide anions and hydrogen peroxide in grapevine leaves. Together, these findings point to the pivotal role of VvbHLH036 and VvThrC1 in the cold stress response in grapes by regulating threonine biosynthesis.
{"title":"VvbHLH036, a basic helix–loop–helix transcription factor regulates the cold tolerance of grapevine","authors":"Yujun Hou, Darren C J Wong, Xiaoming Sun, Qingyun Li, Huimin Zhou, Lin Meng, Xiaoli Liao, Zhenchang Liang, Rishi Aryal, Qingfeng Wang, Haiping Xin","doi":"10.1093/plphys/kiae483","DOIUrl":"https://doi.org/10.1093/plphys/kiae483","url":null,"abstract":"Cold stress is an adverse environmental factor that limits the growth and productivity of horticulture crops such as grapes (Vitis vinifera). In this study, we identified a grapevine cold-induced basic helix–loop–helix (bHLH) transcription factor (VvbHLH036). Overexpression and CRISPR/Cas9-mediated knockout (KO) of VvbHLH036 enhanced and decreased cold tolerance in grapevine roots, respectively. Transcriptome analysis of VvbHLH036-overexpressed roots identified threonine synthase (VvThrC1) as a potential downstream target of VvbHLH036. We confirmed that VvbHLH036 could bind the VvThrC1 promoter and activate its expression. Both the transcripts of VvThrC1 and the content of threonine were significantly induced in the leaves and roots of grapevine under cold treatment compared to controls. Conversely, these dynamics were significantly suppressed in the roots of CRISPR/Cas9-induced knockout of VvbHLH036. These observations support the regulation of threonine accumulation by VvbHLH036 through VvThrC1 during cold stress in grapevine. Furthermore, overexpression and CRISPR/Cas9-mediated knockout of VvThrC1 also confirmed its role in regulating threonine content and cold tolerance in transgenic roots at low temperature. Exogenous threonine treatment increased cold tolerance and reduced the accumulation of superoxide anions and hydrogen peroxide in grapevine leaves. Together, these findings point to the pivotal role of VvbHLH036 and VvThrC1 in the cold stress response in grapes by regulating threonine biosynthesis.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":null,"pages":null},"PeriodicalIF":7.4,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142170930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jason Gregory, Xue Liu, Zongliang Chen, Cecilia Gallardo, Jason Punskovsky, Gabriel Koslow, Mary Galli, Andrea Gallavotti
The formation of the plant body proceeds in a sequential post-embryonic manner through the action of meristems. Tightly coordinated meristem regulation is required for development and reproductive success, eventually determining yield in crop species. In maize (Zea mays), the RAMOSA1 ENHANCER LOCUS2 (REL2) family of transcriptional corepressors includes four members, REL2, RELK1 (REL2-LIKE1), RELK2, and RELK3. In a screen for rel2 enhancers, we identified shorter double mutants with enlarged ear inflorescence meristems (IMs) carrying mutations in RELK1. Expression and genetic analysis indicated that REL2 and RELK1 cooperatively regulate ear IM development by controlling genes involved in redox balance, hormone homeostasis, and differentiation, ultimately tipping the meristem toward an environment favorable to expanded expression of the ZmWUSCHEL1 gene, which encodes a key stem-cell promoting transcription factor. We further demonstrated that RELK genes have partially redundant yet diverse functions in the maintenance of various meristem types during development. By exploiting subtle increases in ear IM size in rel2 heterozygous plants, we also showed that extra rows of kernels are formed across a diverse set of F1 hybrids. Our findings reveal that the REL2 family maintains development from embryonic initiation to reproductive growth and can potentially be harnessed for increasing seed yield in a major crop species.
{"title":"Transcriptional corepressors in maize maintain meristem development","authors":"Jason Gregory, Xue Liu, Zongliang Chen, Cecilia Gallardo, Jason Punskovsky, Gabriel Koslow, Mary Galli, Andrea Gallavotti","doi":"10.1093/plphys/kiae476","DOIUrl":"https://doi.org/10.1093/plphys/kiae476","url":null,"abstract":"The formation of the plant body proceeds in a sequential post-embryonic manner through the action of meristems. Tightly coordinated meristem regulation is required for development and reproductive success, eventually determining yield in crop species. In maize (Zea mays), the RAMOSA1 ENHANCER LOCUS2 (REL2) family of transcriptional corepressors includes four members, REL2, RELK1 (REL2-LIKE1), RELK2, and RELK3. In a screen for rel2 enhancers, we identified shorter double mutants with enlarged ear inflorescence meristems (IMs) carrying mutations in RELK1. Expression and genetic analysis indicated that REL2 and RELK1 cooperatively regulate ear IM development by controlling genes involved in redox balance, hormone homeostasis, and differentiation, ultimately tipping the meristem toward an environment favorable to expanded expression of the ZmWUSCHEL1 gene, which encodes a key stem-cell promoting transcription factor. We further demonstrated that RELK genes have partially redundant yet diverse functions in the maintenance of various meristem types during development. By exploiting subtle increases in ear IM size in rel2 heterozygous plants, we also showed that extra rows of kernels are formed across a diverse set of F1 hybrids. Our findings reveal that the REL2 family maintains development from embryonic initiation to reproductive growth and can potentially be harnessed for increasing seed yield in a major crop species.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":null,"pages":null},"PeriodicalIF":7.4,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142166641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Navigating the shadows: SlCPK10 mediated flower abscission in tomatoes under low light.","authors":"Prateek Jain","doi":"10.1093/plphys/kiae439","DOIUrl":"https://doi.org/10.1093/plphys/kiae439","url":null,"abstract":"","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":null,"pages":null},"PeriodicalIF":7.4,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142170695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yanyan Zhu, Xinlei Wang, Yan He, Yajing Liu, Runze Wang, Yongsheng Liu, Songhu Wang
Chromosome doubling-induced polyploidization is a popular tool for crop breeding. Polyploidy crops commonly have multiple advantages, including increased biomass and stress tolerance. However, little is known about the genes responsible for these advantages. We found kiwifruit (Actinidia chinensis cv. Hongyang) PECTIN METHYLESTERASE 2 (AcPME2)is substantially upregulated in artificially created tetraploid plants that show increased biomass and enhanced tolerance to osmotic stress. Overexpression (OE) of AcPME2 led to increased biomass and enhanced stress tolerance in Arabidopsis (Arabidopsis thaliana), tomato (Solanum lycopersicum), and kiwifruit. Upon short-term osmotic stress treatment, AcPME2-OE plants showed higher levels of demethylesterified pectins and more Ca2+ accumulation in the cell wall than Col-0 plants, which led to increased cell wall stiffness. The stress-induced plasmolysis assays indicated that AcPME2 dynamically mediated the cell wall stiffness in response to osmotic stress, which is dependent on Ca2+ accumulation. Transcriptomic analysis discovered that dozens of stress-responsive genes were significantly upregulated in the AcPME2-OE plants under osmotic stress. Besides, AcPME2-mediated cell wall reinforcement prevented cell wall collapse and deformation under osmotic stress. Our results revealed a single gene contributes to two advantages of polyploidization (increased biomass and osmotic stress tolerance) and that AcPME2 dynamically regulates cell wall stiffness in response to osmotic stress.
{"title":"Chromosome doubling increases PECTIN METHYLESTERASE 2 expression, biomass, and osmotic stress tolerance in kiwifruit","authors":"Yanyan Zhu, Xinlei Wang, Yan He, Yajing Liu, Runze Wang, Yongsheng Liu, Songhu Wang","doi":"10.1093/plphys/kiae475","DOIUrl":"https://doi.org/10.1093/plphys/kiae475","url":null,"abstract":"Chromosome doubling-induced polyploidization is a popular tool for crop breeding. Polyploidy crops commonly have multiple advantages, including increased biomass and stress tolerance. However, little is known about the genes responsible for these advantages. We found kiwifruit (Actinidia chinensis cv. Hongyang) PECTIN METHYLESTERASE 2 (AcPME2)is substantially upregulated in artificially created tetraploid plants that show increased biomass and enhanced tolerance to osmotic stress. Overexpression (OE) of AcPME2 led to increased biomass and enhanced stress tolerance in Arabidopsis (Arabidopsis thaliana), tomato (Solanum lycopersicum), and kiwifruit. Upon short-term osmotic stress treatment, AcPME2-OE plants showed higher levels of demethylesterified pectins and more Ca2+ accumulation in the cell wall than Col-0 plants, which led to increased cell wall stiffness. The stress-induced plasmolysis assays indicated that AcPME2 dynamically mediated the cell wall stiffness in response to osmotic stress, which is dependent on Ca2+ accumulation. Transcriptomic analysis discovered that dozens of stress-responsive genes were significantly upregulated in the AcPME2-OE plants under osmotic stress. Besides, AcPME2-mediated cell wall reinforcement prevented cell wall collapse and deformation under osmotic stress. Our results revealed a single gene contributes to two advantages of polyploidization (increased biomass and osmotic stress tolerance) and that AcPME2 dynamically regulates cell wall stiffness in response to osmotic stress.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":null,"pages":null},"PeriodicalIF":7.4,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142160798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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