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IDENTIFICATION OF MYCOBIOTA AND DIAGNOSIS OF SOYBEAN SEED DISEASES 大豆种子病害真菌菌群鉴定与诊断
Pub Date : 2022-02-19 DOI: 10.31548/agr.13(1).2022.44-50
M. Pikovskyi, Mykhailo Solomiichuk
Micromycetes that colonise soybean seeds reduce their sowing quality, causing loss of germination, rotting and death of seedlings, and the appearance of diseases on young plants. Infection-free seed is one of the factors that ensure crop growth. Therefore, the study of mycobiota of seeds is relevant for the implementation of preventive disease control measures. The purpose of this study was to identify phytopathogenic fungi that colonise soybean seeds and to establish their species composition. The seed samples were selected in the conditions of the Ukrainian Scientific Plant Quarantine Research Station (Chernivtsi Oblast) of the Institute of Plant Protection of the National Academy of Agrarian Sciences during 2019-2021. Mycological studies of soybean seeds were performed in the scientific laboratory of the Department of Phytopathology of the National University of Life and Environmental Sciences of Ukraine using the biological method from subsequent morphological and microscopic analysis of pathogens. It was established that the species composition of mycobiota of soybean seeds is diverse and is represented by 17 species: Reronospora manshurica (Naum.) Syd. (downy mildew pathogen), Sclerotinia sclerotiorum de Bary (white mold pathogen), Botryotinia fuckeliana Whetzel. (grey mold pathogen), Diaporthe sp. (Phomopsis pathogen); pathogens of fusariosis are Fusarium okhusporum (Schl.), Snyd. et Hans., Fusarium graminearum Schwabe, Fusarium gibbosum App. et Wr., Fusarium solani (Mart.) Sacc.; Alternaria alternata (Fries: Fries) Keissler (Alternaria pathogen), Cladosporium cladosporioides (Fresen.) G.A. de Vries (cladosporiosis pathogen), Colletotrichum sp. (anthracnose pathogen); pathogens of various moulds – Aspergillus niger van Tiegh., Aspergillus flavus Link, Trichothecium roseum Link ex Friet Fries, Mucor mucedo Fres. amend. Bref., Penicillium expansum Link and Rhizopus nigricans Ehrenb. The dominant species were C. cladosporioides and A. alternata, their frequency of occurrence was in the range of 28.5-47.8%. The obtained data on the symptoms of mycosis of soybean seeds, the species composition of pathogens and their frequency of occurrence are of practical importance for the diagnosis of diseases, the identification of pathogens and the implementation of preventive therapeutic control measures
寄生在大豆种子上的微霉菌降低了大豆的播种质量,导致种子发芽失败、幼苗腐烂和死亡,以及幼嫩植物出现疾病。无侵染种子是保证作物生长的因素之一。因此,对种子真菌菌群的研究对实施预防疾病控制措施具有重要意义。本研究的目的是鉴定定殖大豆种子的植物致病真菌并确定其物种组成。种子样本于2019-2021年在国家农业科学院植物保护研究所乌克兰科学植物检疫研究站(切尔诺夫茨州)的条件下选择。大豆种子的真菌学研究是在乌克兰国立生命与环境科学大学植物病理学系的科学实验室中进行的,使用随后对病原体进行形态学和显微镜分析的生物学方法。结果表明,大豆种子真菌菌群的种类组成较为多样化,主要有17种:悉德。白霉菌菌核菌(Sclerotinia sclerotiorum de Bary), fuckeliana wheetzel。(灰霉病原菌),Diaporthe sp.(苔藓病原菌);镰刀菌病的病原有镰刀菌(Fusarium okhusporum, Schl.)、镰刀菌(Snyd.)。汉斯。,谷草镰刀菌,赤霉病菌,等。茄枯菌(镰刀菌)Sacc。交替枝孢霉(株);交替枝孢霉(株);G.A. de Vries(枝孢子虫病病原体),炭疽菌(炭疽病病原体);各种霉菌的病原体-黑曲霉。,黄曲霉链接,玫瑰毛霉链接前炸薯条,Mucor mucedo Fres。修改。Bref。、膨胀青霉和黑根霉。优势种为枝孢子蠓和互花蠓,发生频率在28.5 ~ 47.8%之间。所获得的大豆种子真菌病的症状、病原菌种类组成及其发生频率的资料,对疾病的诊断、病原菌的鉴定和预防治疗控制措施的实施具有实际意义
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引用次数: 1
CREATION OF HIGHLY PRODUCTIVE FORMS OF WINTER WHEAT WITH COMPLEX RESISTANCE AGAINST DISEASES AND PESTS ON ARTIFICIAL INFECTIOUS BACKGROUNDS OF THEIR CAUSATIVE AGENTS 利用病原菌的人工感染背景,培育具有复杂抗病能力的高产冬小麦品种
Pub Date : 2022-02-15 DOI: 10.31548/agr.13(1).2022.35-43
T. Mukha, L. Murashko, O. Humenyuk, Y. Suddenko, N. Novytska, O. Martynov
Diseases of field crops substantially reduce the yield and quality of the obtained agricultural products. Underselection of gross grain harvest due to diseases and pests can reach about 25-30% annually. This necessitates the creation of resistant varieties for winter wheat selection. The purpose of this study was to create a new highly productive selection material with complex resistance against the main most harmful diseases for further use in the selection of winter wheat. In the phase of emergence of plants in the tube, winter wheat plants were infected with spores of the causative agent of brown rust according to E.E. Heschele’s method. To create an artificial infectious background of brown rust, a synthetic population of the pathogen (Institute of Plant Protection of the National Academy of Agrarian Sciences) was used as a reservoir of infection – a susceptible variety of Myronivska 10. To create an artificial infectious background of powdery mildew, a local population and an infectious reservoir of the American variety Keprock were used. In the F3 hybrid nursery against the provoking background of powdery mildew, combinations highly resistant to the pathogen (up to 5%) were selected, created with sources of resistance: Zdar, Fakon, Pi170911, Bongo – Svitanok Myronivskyi/Zdar, Kolos Myronivshchyny/Fakon, Berehynia Myronivska/Pi170911, Dostatok/Bongo. Resistance against the pathogen (up to 10.0%) was observed in two crossing combinations (Gorlytsia Myronivska/Gloria and Remeslivna/Wervok). Crossing combinations created with sources of resistance were selected on the artificial infectious background of hybrids of the fourth generation for resistance to brown rust: Flex, V 1275, Tobarzo, 203-238. It is worth noting the hybrids: Oberih Myronivskyi/Flex, Smuhlianka/V 1275, Monotyp/Tobarzo, Kolos Myronivshchyna/203-238. The largest number of grains in an ear was obtained from the combination Svitanok Myronivskyi/Zdar (61.5 pieces), and the largest weight of grain from an ear was obtained from the combinations Horlytsia Myronivska/Gloria and Berehynia Myronivska/Pi170911 (2.38 g and 2.37 g, respectively). The Oberih Myronivskyi/Flex combination stood out for its resistance to brown rust and performance elements. The samples selected based on the results of the study are used as valuable raw material to create new winter wheat varieties resistant to diseases in the Forest Steppe of Ukraine
大田作物病害大大降低了所获得农产品的产量和质量。每年因病虫害造成的粮食总产量选择不足可达25-30%。这就需要为冬小麦的选择创造出具有抗性的品种。本研究旨在为冬小麦选育提供一种对主要有害病害具有复合抗性的新型高产选育材料。根据e.e.h eschele的方法,在试管植株出苗期对冬小麦植株进行了褐锈病菌孢子侵染。为了创建一个人造的褐锈病感染背景,利用一个病原体的合成种群(国家农业科学院植物保护研究所)作为感染库——一个易感品种Myronivska 10。为了创建白粉病的人工感染背景,使用了当地人群和美国品种Keprock的感染库。在白粉病发生背景下的F3杂交苗圃中,选择了对病原菌高度抗性(高达5%)的组合,抗性来源为:Zdar、Fakon、Pi170911、Bongo - Svitanok Myronivskyi/Zdar、Kolos Myronivshchyny/Fakon、Berehynia Myronivska/Pi170911、Dostatok/Bongo。两个杂交组合(Gorlytsia Myronivska/Gloria和Remeslivna/Wervok)对病原菌的抗性最高达10.0%。以第4代杂交种Flex、V 1275、Tobarzo、203 ~ 238为抗褐锈病的人工感染背景,选择具有抗性来源的杂交组合。值得注意的是混合动力车:Oberih Myronivskyi/Flex, Smuhlianka/V 1275, monotype /Tobarzo, Kolos Myronivshchyna/203-238。单穗粒数以Svitanok Myronivskyi/Zdar最多(61.5粒),单穗粒重以Horlytsia Myronivska/Gloria和Berehynia Myronivska/Pi170911最多(分别为2.38 g和2.37 g)。Oberih Myronivskyi/Flex组合因其抗褐锈和性能元素而脱颖而出。根据研究结果选择的样品被用作在乌克兰森林草原培育抗病冬小麦新品种的宝贵原料
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引用次数: 0
EFFECTIVENESS OF SOYBEAN INOCULATION WITH NODULE BACTERIA AND MYCORRHIZAL FUNGI IN THE CONDITIONS OF THE RIGHT BANK FOREST STEPPE OF UKRAINE 在乌克兰右岸森林草原条件下接种大豆根瘤菌和菌根真菌的效果
Pub Date : 2022-02-15 DOI: 10.31548/agr.13(1).2022.27-34
A. Datsko, L. Kvasnitska, O. Litvinova
The relevance of this study is determined by a complex of factors that shape soybean grain productivity – the effectiveness of symbiotic systems formed with nodule bacteria and mycorrhizal fungi, the activity of growth processes and the formation of vegetative organs by soybean plants, as well as their adaptive plasticity under the influence of various external factors. The purpose of this study was to investigate the morphobiometric characteristics of soybean plants, the productivity of the crop after pre-sowing treatment with biological preparations Rhizoline, Mycofriend, and Rhizosave. This paper presents the results of research on the use of soybean seed treatment with biological preparations to improve the growth and development of soybean plants and increase crop productivity. Field, laboratory, mathematical-statistical, and calculation methods were employed for this study. It was found that seed inoculation positively affects the leaf surface area in the budding phase, it increased from 5,313 cm2 in the control to 6,426 cm2 with the Rhizoline + Rhizosave + Mycofriend option, and in the phase of pod formation, respectively, from 8,704 cm2 to 1,113 cm2 . The joint use of Rhizoline + Mycofriend + Rhizosave preparations most contributed to the increase in the mass of the root with nodules (+0.95 g), the number of nodules on the plant (+32 pcs), and the mass of nodules from the plant (+0.3 g). It was found that a comparable situation occurred during the phase of bean formation. The regularity of yield growth during the years under study showed that the average increase in yield varied from 0.41 t/ha with Rhizoline + Rhizosave inoculation to 0.6 t/ha with the combined use of Rhizoline + Rhizosave and the mycorrhizal preparation Mycofriend. It was substantiated that under appropriate conditions, the intensity of symbiotic nitrogen fixation increases thanks to nodule bacteria, as well as the absorption of water and nutrients by the root system of plants due to the activity of mycorrhizal fungi, the entry of amino acids, phytohormones, and vitamins into the plant, which positively affects the growth and development of soybean plants. The materials of this paper are of practical value for farmers in the development of new plant nutrition strategies involving biological preparations in the cultivation technology
本研究的相关性是由影响大豆籽粒产量的复杂因素决定的——大豆与根瘤菌和菌根真菌形成的共生系统的有效性,大豆植物生长过程和营养器官形成的活性,以及它们在各种外部因素影响下的适应性可塑性。本研究的目的是研究播前生物制剂根碱、真菌friend和根皂苷处理对大豆植株形态生物学特性和产量的影响。本文介绍了利用生物制剂处理大豆种子,促进大豆植株生长发育和提高作物产量的研究结果。本研究采用现场、实验室、数理统计和计算方法。结果表明,接种种子对出芽期叶表面积有显著的正向影响,根碱+根腐素+真菌朋友组的叶表面积从对照的5313 cm2增加到6426 cm2,结荚期叶表面积分别从8704 cm2增加到1113 cm2。根碱+菌友+根皂制剂联合使用对根瘤质量(+0.95 g)、根瘤数量(+32个)和根瘤质量(+0.3 g)的增加贡献最大。在豆类形成阶段发现了类似的情况。研究年份的产量增长规律表明,接种根腐碱+根腐碱的产量平均增加0.41 t/ hm2,联合使用根腐碱+根腐碱和菌根制剂Mycofriend的产量平均增加0.6 t/ hm2。结果表明,在适宜条件下,根瘤菌的存在增加了共生固氮的强度,菌根真菌的活性增加了植物根系对水分和养分的吸收,氨基酸、植物激素和维生素的进入对大豆植株的生长发育产生了积极的影响。本文的研究成果对农民开发涉及生物制剂的植物营养新策略具有实用价值
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引用次数: 0
ECONOMIC AND ENERGY EFFICIENCY OF TECHNOLOGIES FOR GROWING MAIZE HYBRIDS DEPENDING ON THE TYPE AND METHODS OF APPLYING FERTILISERS 玉米杂交技术的经济和能源效率取决于施肥的类型和方法
Pub Date : 2022-01-01 DOI: 10.31548/agr.13(1).2022.7-16
T. Antal, R. Hovenko, V. Kalenskyi, Olena Kashtanova, S. Kalenska
The urgency of solving the problem of the growth of maize grain production is unconditional, considering the needs of humanity, the economic, and energy efficiency of cultivation technologies, which substantially depends on the types of fertilisers and the frequency of their application. The purpose of this study was to prove the efficiency of maize grain production by perfecting the fertilisation system. Field research was conducted at the “Bohatyrivske” farm in the Romen district of the Sumy Oblast during 2019-2021 on dark grey podsolised soil. Mathematical and statistical processing was performed using the software package SAS 9.4 and “Agrostat 6”. The economic and energy evaluation of technologies was performed using the calculation method. Hybrids are flexible to growing conditions, respond positively to nutrition optimisation – yield varies from 6.07-6.16 in the control to 8.26-8.50 t/ha with carbomidoammonium nitrate 32 on the background of N22Р57К57. With the background application of N22Р57К57, the yield increased by 16.4%. Nitrogen fertilisers over the background provided an added increase in yield by 7.95-18.6%. With the introduction of carbomidoammonium nitrate 32, the yield increase was 2.19 t/ha and 2.44 t/ha or 36.1% and 38.0%, respectively, of the Concord and Asteroid hybrids. The reaction of hybrids to ammonia water and urea is lower compared to carbomidoammonium nitrate. Production costs for growing maize amounted to UAH 20.3-32.3 thous./ha. The introduction of carbomidoammonium nitrate against the background of N22Р57K57 provided a high conditionally net profit – UAH 19.2-19.9 thous./ha. The profitability of maize grain production is quite high – 44-80%. With the addition of Humilin Stimul, the yield increases by 0.23-1.71 t/ha, depending on the combination of fertilisers. With all types of fertilisers, the highest efficiency was obtained with one-time feeding on BBCH 15-17. The coefficient of energy efficiency for the application of fertilisers decreases. The results of this study are important for production and further areas of research, namely the biodiversity of soil microflora
考虑到人类的需求、种植技术的经济和能源效率,解决玉米谷物生产增长问题的紧迫性是无条件的,这在很大程度上取决于肥料的种类和使用频率。本研究的目的是通过完善施肥系统来证明玉米籽粒生产的效率。2019-2021年期间,在苏梅州罗马区的“Bohatyrivske”农场对深灰色灰化土壤进行了实地研究。采用SAS 9.4软件包和“Agrostat 6”软件进行数理统计处理。采用计算方法对各工艺进行了经济和能源评价。杂交种对生长条件具有灵活性,对营养优化反应积极-产量变化范围从对照的6.07-6.16吨/公顷到N22Р57К57背景下硝酸铵32的8.26-8.50吨/公顷。背景应用N22Р57К57,产率提高16.4%。氮肥在背景上提供了7.95-18.6%的额外增产。添加卡米多铵32后,Concord和Asteroid的产量分别提高了2.19 t/ha和2.44 t/ha,分别提高了36.1%和38.0%。与卡米多硝酸铵相比,杂化产物对氨水和尿素的反应较低。种植玉米的生产成本为20.3- 3.23万澳元/公顷。在N22Р57K57背景下引入卡米多硝酸铵提供了高的有条件净利润- UAH 19.2-19.9千/公顷。玉米粮食生产的利润率相当高,达44-80%。施Humilin刺激物后,产量增加0.23-1.71 t/公顷,具体取决于施肥组合。在各种肥料中,一次性饲喂BBCH 15-17的效率最高。施用肥料的能源效率系数降低。该研究结果对生产和进一步的研究领域,即土壤微生物区系的生物多样性具有重要意义
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引用次数: 0
Productivity of seed potatoes with local application of phosphorus and potassium fertilizers 局部施磷、钾肥对种薯生产力的影响
Pub Date : 2021-08-31 DOI: 10.31548/agr2021.02.037
A. Bykin, T. Panchuk
Ukraine is a leader in potato production, among the top three producer countries and ranks second in the world in terms of per capita potato consumption, second only to Belarus. It produces 139 kg per year per capita, with a world average of about 33 kg/person/year. According to UCAB, in recent years the area under potatoes has increased by 2.7% to 1325 thousand hectares. However, the average yield for the last three years was 15.8 t/ha. In some advanced farms of Ukraine the yield reaches 30–40 t/ha due to the introduction of scientific developments in production. However, in general, the potential for economic productivity of potatoes in Ukraine now remains completely untapped. Potato plants are demanding to the presence of nutrients in the soil. Soil nutrients must be available for plants and in sufficient quantity. It causes by the biological characteristics of potatoes. The effectiveness of mineral fertilizers depends on the methods and quality of their application. The traditional method of fertilization involves fertilizers broadcasting on the soil surface with their tillage. As a result of uneven placement of fertilizer granules in the soil layer, the transition of nutrients into unavailable form to plants, which causes the uneven plants development and maturation of tubers. Therefore, one of the ways to improve the potatoes nutrition and reduce nutrient losses and obtain high stable crop yields is the local application of mineral fertilizers in the area of the root system location. Local application of phosphorus determines it better availability throughout the growing season, which provides accelerated growth and development of roots and shoots, as well as the formation of the optimal number of tubers. Local placement of potassium improves its availability and provides enhanced synthesis and transport of carbohydrates in plants, increases the absorption of moisture and nutrients by the roots, strengthens their resistance to disease and improves the quality of tubers. The aim of our researching was to investigate the effectiveness of the phosphorus and potassium application in different methods and phosphorus and potassium rates and to establish it impact in the productivity of seed potatoes. The research was located in the field experiment of the Department of Agrochemistry and Quality of Crop Products named by O.I. Dushechkina NULES of Ukraine on the territory of LLC "Biotech LTD" (Boryspil district, Kyiv region) during 2019–2020. Early-maturing variety Tiras was selected for research. The planting area was 495m2 of the accounting area was 312m2. The experiment was repeated 4 times. The placement of options was systematic. As a result of research it was established that local application of phosphorus and potassium fertilizers provided such a level of yield of Tiras potatoes, which was not inferior to the variant with the fertilizers broadcasting. Yield growth in variants with local application ranged from 0.6 t/ha to 2.9 t/ha, dependin
乌克兰是马铃薯生产的领导者,是前三大生产国之一,人均马铃薯消费量位居世界第二,仅次于白俄罗斯。每年人均产量139公斤,世界平均水平约为33公斤/人/年。根据UCAB的数据,近年来马铃薯种植面积增加了2.7%,达到132.5万公顷。然而,过去三年的平均产量为15.8吨/公顷。在乌克兰的一些先进农场,由于引进了生产方面的科学发展,产量达到30-40吨/公顷。然而,总的来说,乌克兰马铃薯的经济生产力潜力目前仍未完全开发。马铃薯对土壤中的养分要求很高。土壤养分必须能提供给植物,而且数量要足够。它是由马铃薯的生物学特性引起的。矿质肥料的效果取决于施用方法和施用质量。传统的施肥方法是在耕作时将肥料撒播在土壤表面。由于施肥颗粒在土层中的分布不均匀,养分向植物转化为不可用形式,导致植物发育不均匀,块茎成熟不均匀。因此,改善马铃薯营养,减少养分流失,获得作物高产稳产的途径之一是在根系位置区域局部施用矿质肥料。磷的局部施用决定了其在整个生长季节的有效性,这加速了根和芽的生长发育,并形成了最佳数量的块茎。局部施钾可提高钾的利用率,增强植物碳水化合物的合成和运输,增加根系对水分和营养物质的吸收,增强其抗病能力,改善块茎质量。本研究旨在探讨不同施磷方式和施磷、施钾量对种薯产量的影响。该研究于2019-2020年在乌克兰O.I. Dushechkina NULES命名的农业化学和作物产品质量部门在LLC“Biotech LTD”(基辅地区Boryspil区)境内进行的田间试验中进行。选择早熟品种提拉斯作为研究对象。种植面积为495m2,核算面积为312m2。实验重复4次。期权的安排是有系统的。研究结果表明,局部施用磷钾肥可提供提拉斯马铃薯的产量水平,其产量并不低于施用化肥的品种。不同品种在本地施用时的产量增长幅度在0.6 t/公顷至2.9 t/公顷之间,具体取决于施用量。施用P60K135土肥的种子马铃薯产量最高(33.4 t/ hm2),籽粒产量最高。为31.6吨/公顷。
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引用次数: 1
Qualitative characteristics of the fungus Cyclocybe Aegerita Strains 2229, 2230, 2231 under industrial cultivation conditions 工业培养条件下绿绿环孢菌2229、2230、2231的质量特性研究
Pub Date : 2021-07-23 DOI: 10.31548/agr2021.03.085
I. Bandura, A. Kulyk, S. Makohon, O. Tsyz, O. Khareba, V. Khareba, Z. Kovtuniuk
The “poplar” mushroom C. aegerita has fruiting bodies that are attractive for their appearance and tasty properties. It is also known that mushrooms contain unique substances, especially proteins, polysaccharides, and aromatic terpenes with enhanced nutritional and medicinal value. However, the lack of a well-founded technology of cultivation using available agriculture wastes interferes with the domestic market saturation with this mushroom. Three strains of C. aegerita from the IBK mushroom culture collection were investigated on the possibility of introducing them into the industrial culture. The vegetation cycle of strains 2230 and 2231 was found to have a duration of 42 days versus 46-64 days for the previously studied strains. The highest yield was obtained in the study for strain 2231, which yielded from 217 to 220 grams per kilogram of the substrate during three cycles of cultivation. The biological efficiency of the strains as a result of the first fructification flush was from 20,2 (2229) to 60.4 % (2231), which is in agreement with the published data of other investigators. The external appearance was described in detail and parameters of fruiting bodies of strains were analyzed, which differed from each other both by shape and size. Microclimatic conditions for the study, strains 2230 and 2231 had brown caps, whereas the formed fruiting bodies of strain 2229 were distinguished by a pleasing milky cream color. The heaviest fruiting bodies were formed in strain 2229 (7.5±0.4 g), which was an average of 2 g higher than the weight of fungi of other strains studied. The highest fruiting bodies were found in strain 2230 at a height of 91.0±1.8 mm, which also had the widest cap with a 29.5±0.9 mm diameter. The greatest diameter of the stipe, reaching an average of 10.8±0.3 mm, was observed in fruiting bodies of strain 2229. The variability of morphological characters and the tendencies in the number of fruiting bodies of a certain size in the total volume of harvested mushrooms were investigated, which can help in the design of packing and marketing measures.
“杨树”蘑菇C. aegerita的子实体因其外观和美味的特性而具有吸引力。众所周知,蘑菇含有独特的物质,特别是蛋白质、多糖和芳香萜烯,具有增强的营养和药用价值。然而,由于缺乏利用现有农业废弃物进行栽培的可靠技术,这种蘑菇在国内市场的饱和受到了影响。从IBK菌种中分离出3株绿僵菌,研究了将其引入工业培养的可能性。菌株2230和2231的植被周期为42天,而之前研究的菌株为46-64天。菌株2231的产量最高,在三个周期的培养中,每公斤底物的产量为217至220克。经第一次果实冲洗后,菌株的生物效率为20.2%(2229)~ 60.4%(2231),这与其他研究者发表的数据一致。详细描述了不同菌种的子实体外观,分析了不同菌种的子实体形状和大小的参数。在小气候条件下,菌株2230和2231的菇盖呈棕色,而菌株2229形成的子实体呈乳白色。菌株2229的子实体最重(7.5±0.4 g),比其他菌株的子实体重平均高2 g。菌株2230的子实体高度最高,为91.0±1.8 mm,子实体盖最宽,直径为29.5±0.9 mm。菌株2229子实体的茎柄直径最大,平均为10.8±0.3 mm。研究了采菇形态性状的变异和一定大小的子实体在采菇总量中数量的变化趋势,为设计包装和销售措施提供依据。
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引用次数: 0
Determination of pesticide residues by chromatographic methods for food safety 食品安全用色谱法测定农药残留
Pub Date : 2021-06-30 DOI: 10.31548/agr2021.03.111
Nataliia Tereshchenko, О. Kursenko, О. Khyzhan, O. Khyzhan, Oleksandr Bobunov, Lidiia Kovshun
The paper presents the methodology of preparation of samples of oilseeds, lettuce, apples for research by chromatographic control of xenobiotics of the following chemical groups of pesticides: benzimidazole derivatives, anilinopyrimidine derivatives, bipyridylium derivatives. The implementation of the following processes is considered: homogenization of the sample, purification of the extract by solid-phase or liquid-liquid extraction, obtaining a plant extract, obtaining an extract of analytes. For fine-grained homogenized samples of sunflower seeds, the optimal ratio of raw material -extragent is 1:20, for pasty homogenized samples of apple fruit - 1:10, for liquid samples of homogenized lettuce - 1: 5. Analysis of the distribution of xenobiotics in the system octane/water, the dipole moment of solvents allowed to determine the extractants that are able to dissolve and remove xenobiotics from raw materials. It was found that a mixture of acetonitrile and methanol (4: 1) should be used to remove benzimidazole derivatives and anilinopyrimidine derivatives, bipyridylium derivatives are best extracted with methanolic trifluoroacetic acid (9.5: 0.5). Quantitative analysis of xenobiotics content in extracts obtained from samples artificially enriched with xenobiotics was performed. The most complete xenobiotics were removed from samples of plant products containing traces of fat. The most difficult process of sample preparation is the process of obtaining sunflower seed extract. The content of xenobiotics in extracts obtained from samples artificially enriched in analytes is influenced by the temperature at which the process takes place and the duration of extraction. Based on the chemical composition of the sample matrix and the list of analytes, the conditions of the variable component of the methodology are proposed: obtaining plant extract under the action of selective solvents, homogenized raw material-solvent with constant stirring of the extraction system at 180-200 rpm, or under the action of ultrasonic vibrations with a frequency of 37 kHz from 4°C to 25°C for 5-25 minutes. The control of the qualitative and quantitative composition of the studied plant extracts and analyte extracts was investigated by the methods of high-performance liquid and gas chromatography (liquid and gas) with mass-selective detectors.
本文介绍了油籽、生菜、苹果样品的制备方法,并对苯并咪唑衍生物、苯胺嘧啶衍生物、联吡啶衍生物等化学类农药进行了色谱控制。考虑以下过程的实施:样品的均质化,通过固相或液-液萃取纯化提取物,获得植物提取物,获得分析物的提取物。葵花籽细粒均质样品,原料与萃取剂的最佳比例为1:20,苹果果糊状均质样品为1:10,生菜均质液体样品为1:5。分析系统中异种生物的分布辛烷/水,溶剂的偶极矩允许确定萃取剂能够溶解和去除原料中的异种生物。发现苯并咪唑类衍生物和苯胺嘧啶类衍生物的提取应采用乙腈与甲醇(4:1)的混合物,联吡啶类衍生物的提取最好采用甲醇三氟乙酸(9.5:0.5)。从人工富集的样品中提取的提取物中定量分析了外源物的含量。从含有微量脂肪的植物产品样品中除去了最完整的异种生物。样品制备中最困难的工序是提取葵花籽提取物的过程。从人工富集分析物的样品中提取的提取物中所含的异种生物制剂受提取过程发生的温度和提取时间的影响。根据样品基质的化学成分和分析物列表,提出了该方法可变组分的条件:在选择性溶剂的作用下获得植物提取物,在提取系统中以180-200 rpm恒定搅拌均匀的原料-溶剂,或在频率为37 kHz的超声波振动作用下从4°C到25°C,持续5-25分钟。采用高效液相和气相色谱法(液相和气相)和质量选择检测器对所研究植物提取物和分析物提取物的定性和定量组成进行控制。
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引用次数: 1
Evaluation of parsnip varieties (Pastinaca Sativa l.) on productivity and adaptability in the conditions of the Right bank Forest steppe of Ukraine 乌克兰右岸森林草原防风草品种生产力和适应性评价
Pub Date : 2021-06-05 DOI: 10.31548/agr2021.02.069
O. Komar, V. Khareba, I. Fedosiy, O. Khareba
The commodity output of parsnip was formed in the conditions of the Right‑Bank Forest-steppe of Ukraine within the limits of the sum of active temperatures 1356‑1495 °С, the amount of precipitation 171‑318 mm and relative humidity 57‑64 %. The crop yield had an invert dependence on the sum of temperature and the direct dependence on the amount of precipitation, relative humidity of air and hydrothermal coefficient. The highest yield in the conditions of the Right-bank Forest-steppe of Ukraine provided the Stymul and Pulse varieties with a total yield of 46,2 and 44,8 tons/hectare, and the commodity output of roots of 89 and 88 %, accordingly. It was calculated that the varieties Pulse and Stymul had the highest selective value of the genotype (SCGi = 46,2 and 44,8 accordingly) for yield, high ecological stability (Sgi = 3,43 and 1,96 accordingly) and plasticity (bi = 1,47 and 0,89 % accordingly). The highest indicator of the total adaptive capacity for plant productivity was noted in the Stymul (ZAZ = 3,12) and Pulse (ZAZ = 1,76) varieties. According to the indicator of specific adaptive ability, the Stymul variety (SAZ = 2,52) and Pulse (ZAZ = 0,78) was distinguished. According to the biochemical composition of roots, the investigated varieties did not exceed the control. However, in Boris variety the content of dry matter (25,1 %), dry soluble substance (15,8 %) and sugar (6,4 %) was at the control level. All varieties showed high levels of vitamin C – 8,9‑10,1 mg/100 g. The varieties were not susceptible to accumulation of nitrates and their content varied from 67 to 80 mg/kg and was below the maximum acceptable level (MDR 250 mg/kg). The highest tasting score of 5,6 points got the Pulse. To obtain a consistently high root crop yield at the level 44,8‑46,2 tons/hectare and marketability of roots at the level of 88‑89 % with a high content of major biochemical components in the conditions of the Right‑Bank Forest-steppe of Ukraine on medium-podzolic, roughly dusty, easy loamy soil with the usage of high-yielding of the varieties parsnips Pulse and Stymul.
在乌克兰右岸森林草原的活动温度1356 ~ 1495°С、降水量171 ~ 318 mm、相对湿度57 ~ 64%的条件下,形成防风草的商品产量。作物产量与气温和呈负相关,与降水量、空气相对湿度和水热系数有直接关系。乌克兰右岸森林草原条件下的最高产量为Stymul和Pulse品种提供了46,2和44,8吨/公顷的总产量,相应的根茎商品产量为89%和88%。结果表明,“脉冲”和“Stymul”品种在产量、生态稳定性(分别为3、43和1、96)和可塑性(分别为1、47和0.89%)方面具有最高的基因型选择价值(分别为46、2和44.8)。植物生产力总适应能力指标最高的品种是Stymul (ZAZ = 3,12)和Pulse (ZAZ = 1,76)。根据特定适应能力指标,区分Stymul品种(SAZ = 2,52)和Pulse品种(ZAZ = 0,78)。根据根系生化组成,所调查品种均未超过对照。而鲍里斯品种的干物质(25.1%)、干可溶性物质(15.8%)和糖(6.4%)含量均处于对照水平。所有品种的维生素C - 8、9 - 10和1 mg/100 g的含量都很高。这些品种对硝酸盐的积累不敏感,其含量在67 ~ 80 mg/kg之间,低于最高可接受水平(MDR 250 mg/kg)。最高的品尝分数为5,6分,得到了Pulse。在乌克兰右岸森林草原的中灰化土上,采用高产的防风草和Stymul品种,在主要生化成分含量高的条件下,获得44、8 - 46、2吨/公顷的根系作物产量和88 - 89%的根系市场适销性。
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引用次数: 1
Productivity of triticale depending on the content of photosynthesizing pigments at anthesis 小黑麦的产量取决于花期光合色素的含量
Pub Date : 2021-06-04 DOI: 10.31548/agr2021.02.015
B. Mazurenko
Photosynthetic pigments play an important role in the accumulation of dry matter and they can be the markers of stress. Change in the ratio of chlorophyll A and B indicates physiological changes and adaptation of the organism to changes of environmental conditions. Sowing terms and application of nitrogen fertilizers are long-term factors, so the content of chlorophylls in the flag leaf indicates long-term adaptation of the photosynthetic system. Establishing a relationship between the chlorophyll content at anthesis and the accumulation of dry matter in posy-anthesis period indicates the varietal response of triticale to fertilization and sowing dates. It was conducted a three-factor field experiment. There are studied two winter and one facultative triticale cultivar, two autumn sowing terms and fertilization system with application the different rates of nitrogen fertilizers in different growth stages. It was found that the content of photosynthetic pigments in each variety varied depending on sowing term and fertilizer system in terms of mg per 1 g of dry matter for chlorophyll A, B and the amount of A+B. At the same time, the difference between the factors and their interactions was insignificant for the total chlorophyll mass per unit area (g/m2) for chlorophyll A and the amount of A+B, but it was significant for chlorophyll B by the fertilizer system factor. This indicates that the main stress signal is chlorophyll B, so the ratio of chlorophyll A and B differed significantly depending on this factor. Cla: Clb ratio in the optimal sowing period is 5.3–8.1 in cv. Pidzimok kharkivskiy, 4.8–8.3 in cv. Amur and 5.0–6.7 in cv. Obriy mironivskiy. All cultivars have a strong positive correlation between the chlorophyll content at anthesis and accumulation of dry matter at post-anthesis perion under optimal conditions, but facultative triticale Pidzimok kharkivskiy has a negative correlation with unfavorable, due to increased chlorophyll, without increasing dry matter accumulation. Further study of the relationship between chlorophyll content and the synthesis of primary and secondary metabolites is promising in the study of varietal response to stress and agronomic factors.
光合色素在干物质积累中起着重要的作用,可以作为胁迫的标志。叶绿素A和叶绿素B比值的变化表明生物体的生理变化和对环境条件变化的适应。播期和氮肥施用是长期因素,因此旗叶叶绿素含量反映了光合系统的长期适应性。花期叶绿素含量与花期干物质积累之间的关系表明了小黑麦品种对施肥和播期的响应。进行了三因素现场试验。研究了两个冬季和一个兼性小黑麦品种、两个秋播期和不同生育期施用不同氮肥量的施肥制度。各品种光合色素含量随播期和施肥制度的不同而变化,以每1 g干物质中叶绿素A、B和A+B的含量为单位。同时,叶绿素A的单位面积总叶绿素质量(g/m2)和A+B的数量在各因子之间及其相互作用的差异不显著,但对叶绿素B而言,肥料系统因子差异显著。这说明胁迫信号以叶绿素B为主,因此叶绿素A与叶绿素B的比值因胁迫因子的不同而有显著差异。最佳播期Cla: Clb比值为5.3 ~ 8.1 / cv。Pidzimok kharkivskiy, 4.8-8.3 in cv。阿穆尔河和5.0-6.7的cv。Obriy mironivskiy。在最优条件下,各品种花期叶绿素含量与花后干物质积累呈较强的正相关,但兼性小黑麦与叶绿素含量增加而不增加干物质积累呈负相关。进一步研究叶绿素含量与主要代谢物和次级代谢物合成的关系,对研究品种对胁迫和农艺因子的响应具有重要意义。
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引用次数: 0
Long-term monitoring of aridization in agriculture before change and for climate change in Ukraine 乌克兰变化前农业干旱化和气候变化的长期监测
Pub Date : 2021-01-01 DOI: 10.31548/agr2021.04.102
E. Dehodyuk, S. Dehodyuk, Yulia Borko, O. Litvinova, Yu. O. Ihnatenko, A. Mulyarchuk
The results of systematic monitoring in Ukraine of drought manifestations by the method of generalization of spring, summer and autumn droughts conducted by I. Ye. Buchynsky for 1872-1969 and our meteorological generalizations by types of weather (1951-1984) and clearly expressed zonal climate changes (1985-2006) and statistical and local signs of climate aridization at the meteorological station in the northern part of the Forest-Steppe (" Shepherds ") – 2006-2020, covering a total historical period of 150 years. According to the spatial distribution of droughts, determined by a single method, from 1982 to the late 60s of the twentieth century. Mainly in the south of Ukraine, in 2 years. The number of moderate and extensive droughts since the early twentieth century. (11-30 % of the area of distribution), characteristic of the summer period, and they are repeated in the south in 4-5 years. Catastrophic droughts (more than 50%) are characteristic of large areas for every tenth year, which, since the last quarter of the nineteenth century and to the 50s of the twentieth century. Ended in famine for the population (especially the droughts of 1891, 1921, 1947). Subsequent droughts in 1963 and 1968 were characterized by abnormal weather conditions, but without a food crisis, which was associated with the beginning of chemicalization and the increase in agricultural crops. Meteorological indicators of six stations in different climatic zones for 1951-1981 and 1985-2008 indicate a zonal systematic increase in positive air temperatures with decreasing precipitation, the deficit of which is compensated by the system of agriculture with moderate saturation of organic and mineral fertilizers, which have a stabilizing effect extreme years. From the last quarter of the twentieth century. Climate change has been identified, which is related not only to the greenhouse effect, but also to the frequency that depends on the ecliptic of the globe. Particularly noticeable climate change in recent decades, accompanied by a systematic increase in air temperature and increasing aridity of the climate, and requires humanity to new technical and technological solutions.
叶氏(I. Ye.)采用春、夏、秋干旱概化方法对乌克兰干旱表现进行系统监测的结果。布金斯基(Buchynsky)的1872-1969年,以及我们按天气类型的气象概括(1951-1984),并清楚地表达了地域性气候变化(1985-2006)和2006-2020年森林草原北部(牧羊人)气象站气候干旱化的统计和局部迹象,涵盖了150年的总历史时期。根据1982年至20世纪60年代末的单一方法确定的干旱空间分布。主要是在乌克兰南部,在两年内。自二十世纪初以来,中度和广泛干旱的次数。(分布面积的11- 30%),以夏季为特征,在南方每4-5年重复发生一次。从19世纪最后25年到20世纪50年代,灾难性干旱(超过50%)是每10年大面积发生一次的特征。以饥荒结束(尤其是1891年、1921年和1947年的干旱)。随后的1963年和1968年的干旱以异常的天气条件为特征,但没有发生粮食危机,这与化学化的开始和农作物的增加有关。1951—1981年和1985—2008年不同气候带6个站点的气象指标表明,正气温呈地带性系统增加,降水减少,正气温的不足由有机肥和矿肥适度饱和的农业系统弥补,在极端年份具有稳定作用。从20世纪最后25年开始。气候变化已被确定,它不仅与温室效应有关,而且与取决于地球黄道的频率有关。近几十年来,气候变化尤其引人注目,伴随着气温的系统性升高和气候的日益干旱,需要人类提出新的技术和技术解决方案。
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引用次数: 0
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