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ALGINATE BEADS UTILIZATION FOR LONG-TERM STORAGE OF MICROALGAL ISOLATES 藻酸盐珠在微藻分离株长期贮藏中的应用
IF 1.4 Q3 PLANT SCIENCES Pub Date : 2023-08-31 DOI: 10.54910/sabrao2023.55.4.22
T. Al-Someidae
Microalgae reckon one of the most promising organisms due to their numerous applications in biotechnology, such as, their biomass utilization to extract various primary and secondary metabolites. These compounds benefit the food and pharmaceutical industries and the energy field, such as, biofuel and hydrogen gas production. Therefore, it is necessary to find various sustainable ways to actively preserve the isolates and productive strains with purity for an extended period without affecting their genetic characteristics and productive ability to grow and regenerate. The latest study aimed to compress several microalgae to form alginate beads using sodium alginate with five types of pure algal cultures, i.e., Scenedesmus quadricauda, Scenedesmus dimorphus, Chlorella vulgaris, Chlorococcum humicola, and Chlamydomonas sp. The vitality and activity of all the strains studied through the storage period showed the success of preparing alginate beads and staying viable for 18 months at 4 °C in the refrigerator under dark conditions. Therefore, encapsulating microalgae with sodium alginate is a possible and helpful method for preserving algae isolates for a prolonged period in a pure form. The survival of algae in alginate beads is an essential step to apply in the future as one of the viable methods to preserve pure algae isolates for a long time.
微藻被认为是最有前途的生物之一,因为它们在生物技术中有许多应用,例如利用生物量提取各种初级和次级代谢产物。这些化合物有利于食品和制药行业以及能源领域,如生物燃料和氢气生产。因此,有必要找到各种可持续的方法,在不影响其遗传特性和生长再生的生产能力的情况下,长期有效地保持分离株和生产菌株的纯度。最新的研究旨在使用海藻酸钠将几种微藻与五种类型的纯藻类培养物(即四头藻Scenedesmus quadrauda、二形藻Scenedsmus dimorphus、小球藻Chlorella vulgaris、绿球藻Chlorococcum humicola和衣藻Chlamydomonas sp。在整个储存期内研究的所有菌株的活力和活性表明,成功地制备了海藻酸盐珠,并在黑暗条件下在4°C的冰箱中保持了18个月的活力。因此,用藻酸钠包裹微藻是一种可能且有助于以纯形式长期保存藻类分离物的方法。藻类在藻酸盐珠中的存活是未来应用的重要步骤,是长期保存纯藻类分离物可行的方法之一。
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引用次数: 0
PHYSIOLOGICAL AND BIOCHEMICAL PARAMETERS OF SOYBEAN GENOTYPES UNDER DIVERSE WATER REGIMES 不同水分条件下大豆基因型的生理生化参数
IF 1.4 Q3 PLANT SCIENCES Pub Date : 2023-08-31 DOI: 10.54910/sabrao2023.55.4.7
H. Matniyazova, S. Nabiev, M. Kholikova, G. Mavlonova, Prof. Naqib Ullah, Khan
This study pursued tests on 17 local and exotic soybean (Glycine max L. Merr.) cultivars with two different water regimes. In the optimal irrigated condition (control), the soybean plants gained five times irrigation in the scheme of 1:3:1 during the vegetation period, with the total volume of water used for that irrigation being 5000–5500 m3 /ha. For the water deficit condition, irrigation was only three times on the scheme 1:1:1, using a total volume of water at 3000–3500 m3 /ha. In the optimal irrigated condition, water provision was only once during the formation of soybean leaves, three times during the budding period, and once again at the beginning of the blooming period. In that condition, the provision of an artificially created water deficit condition and drought with one irrigation ensued at the beginning of the blossoming period. According to the results, chlorophyll "b" pigment was more sensitive to water deficit conditions than chlorophyll "a.” The increase in chlorophyll "b" under drought conditions increased chlorophyll’s protection function, the primary photosynthetic pigment, with its reception of photons from sunlight. Stress enzyme activity increased to different extents compared with optimal water supply conditions in the soybean cultivars under water deficit conditions. The number of chloroplast pigments in plant leaves and the activity of peroxidase, catalase, and superoxide dismutase enzymes in leaves strongly influenced local and exotic soybean genotypes during the blooming-harvest period under drought conditions. With water deficit conditions, the number of pods per plant and 1000-grain weight reduced from 8.6% to 63.7% and 1.7% to 12.8% in the local and 3.8% to 28.3% and 5.6% to 58.8% in exotic soybean cultivars compared with water optimal conditions.
本研究对17个本地和外来大豆(Glycine max L. Merr.)品种在两种不同的水分制度下进行了试验。在最佳灌溉条件(对照)下,黄豆植物在植被期按1:3:1的灌溉方案进行5次灌溉,每次灌溉的总水量为5000 ~ 5500 m3 /ha。在缺水条件下,1:1:1方案仅灌溉3次,总水量为3000 ~ 3500m3 /ha。在最佳灌溉条件下,大豆叶片形成期仅需一次供水,出芽期仅需三次供水,开花初期仅需一次供水。在这种情况下,在花期开始时,提供人为造成的缺水条件和一次灌溉造成的干旱。结果表明,叶绿素b比叶绿素a对水分亏缺条件更敏感,干旱条件下叶绿素b含量的增加增加了叶绿素的保护功能,增强了叶绿素对阳光光子的接收能力。水分亏缺条件下大豆品种的胁迫酶活性均有不同程度的提高。干旱条件下,植物叶片中叶绿体色素的数量以及叶片中过氧化物酶、过氧化氢酶和超氧化物歧化酶的活性对本地和外来大豆基因型有强烈的影响。在水分亏缺条件下,与水分最优条件相比,本地大豆单株荚果数和千粒重分别从8.6%降至63.7%和1.7%降至12.8%,外来大豆品种分别从3.8%降至28.3%和5.6%降至58.8%。
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引用次数: 0
AGROTECHNOLOGY FOR FEED CULTIVATION AND CREATION OF HAYFIELDS AND PASTURES IN THE FOREST AND STEPPE ZONE OF NORTHERN KAZAKHSTAN 哈萨克斯坦北部森林和草原地区饲料种植和干草和牧场的农业技术
IF 1.4 Q3 PLANT SCIENCES Pub Date : 2023-08-31 DOI: 10.54910/sabrao2023.55.4.18
А. Shayakhmetova, I. Savenkova, В. Nasiyev, М. Akhmetov, А. Useinov, А. Taskulova, А. Temirbulatova, Dr. Osama Osman Atallah
A high-quality feed base is a must to ensure the growing of numerous animals via selecting promising high-yielding crops that can provide livestock with feeds at the entire production stage. This study purposed to develop new technologies for reinstating a balanced use of pastures in Kazakhstan’s steppe and forest-steppe zones. Several investigations succeeded in 2019–2022 at the Service-ZHARS Limited Liability Partnership (LLP) production fields of District Kyzylzhar, North Kazakhstan. Five chosen pasture combinations created multifactor pasture lands, as follows: common alfalfa + Festulolium; awnless brome + yellow sweet clover; common alfalfa + pasture ryegrass + Festulolium; white sweet clover + awnless brome + Timothy grass, and sainfoin + Festulolium + pasture ryegrass + Timothy grass. For haymaking and pasture chain construction, the following grass combinations selected comprised two-pasture grasses (previous years' brome and Timothy grass + common alfalfa + sainfoin) and seven hay grasses (vetch + oats, Sudan grass, sorghum-Sudan grass hybrid + sorghum, corn for silage, sorghum, peas + oats + barley + wheat, and peas). The use of droughtresistant legumes, cereals, and arable crops and their mixtures positively impacted intensifying the feed base and reducing the pasture areas’ degradation. Thus, in the first experiment, the green mass collection was higher than 3.33 t ha-1 , while in the second one, it was below 4.75 t ha-1 , which fully bestowed the physiological needs of animals. The species diversity of pasture vegetation has improved because of beans’ inclusion, possibly enhancing the protein ratio in green feed and hay as the main component of the farm animals' diet.
通过选择有前途的高产作物,在整个生产阶段为牲畜提供饲料,必须有一个高质量的饲料基地,以确保大量动物的生长。本研究旨在开发新技术,以恢复哈萨克斯坦草原和森林草原带牧场的平衡利用。2019-2022年,在哈萨克斯坦北部Kyzylzhar地区的Service-ZHARS有限责任合伙公司(LLP)生产领域进行了几次成功的调查。5种选择的草场组合形成了多因子草场:普通紫花苜蓿+羊鞭草;无芒雀花+黄色甜三叶草;普通紫花苜蓿+牧场黑麦草+羊鞭草;白甜三叶草+无芒雀麦+蒂莫西草,以及红豆草+ Festulolium +牧场黑麦草+蒂莫西草。在制干草和牧场链建设方面,选择了以下牧草组合,包括两种牧草(前些年的雀麦和蒂莫西草+普通苜蓿+红豆草)和七种干草(野豌豆+燕麦、苏丹草、高粱-苏丹草杂交品种+高粱、青贮玉米、高粱、豌豆+燕麦+大麦+小麦和豌豆)。使用抗旱豆类、谷物和可耕地作物及其混合物对强化饲料基础和减少牧区退化有积极影响。因此,在第一次实验中,绿色质量收集高于3.33 t ha-1,而在第二次实验中,绿色质量收集低于4.75 t ha-1,充分满足了动物的生理需求。大豆的加入改善了牧场植被的物种多样性,可能提高了作为农场动物日粮主要成分的青饲料和干草的蛋白质比例。
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引用次数: 0
PRODUCTIVITY ASSESSMENT OF VARIOUS PLANT COMMUNITIES AT URANIUM MINE SITES IN CENTRAL KAZAKHSTAN 哈萨克斯坦中部铀矿场各种植物群落的生产力评估
IF 1.4 Q3 PLANT SCIENCES Pub Date : 2023-06-30 DOI: 10.54910/sabrao2023.55.3.21
A. Bugubaeva, A. Kuprijanov, V. Chashkov, S. Kuanyshbaev, K. Valiev, S. Mamikhin, A. Shcheglov, A. Nugmanov, A. Bulaev, G. Sultangazina, K. Kunanbayev, O. Chernyavskaya, G. Baubekova, G. Ruchkina, O. Safronova, M. Uxikbayeva, Y. Sokharev
Examining the overgrowth of dumps, techno-soils, and areas containing waste products of uranium mines is vital for understanding the dynamic features of vegetation cover in technogenic landscapes. The main aim of this study was to investigate variations in the plant species composition and their productivity in dumps and technological areas and the intensity of soil ionizing radiations under varying environmental conditions based on the Shantobe Uranium Deposit, Kazakhstan. The vegetation at the waste dumps and technogenic sites is in the early stages of syngenesis and is representative of pioneer and group-thicket communities. Adverse ecological conditions associated with intense sulfate salinization formation thrive at the technological sites. However, the floristic composition is illustrative of highly resistant species (Calamagrostis epigejos and Phragmites australis) and secondary species. Typically the formation of steppe zone plant communities of Kazakhstan does not occur in these sites. The productivity of the recultivated dump and banks of the former uranium mine is quite high at 120–150 g/m2, which matches the meadow-ruderal communities of Northern Kazakhstan. However, the lowest productivity of 30–37 g/m2 emerged in the non-recultivated and partially processed sulfuric acid heap leaching stacks containing uranium ore, which create exceedingly unfavorable conditions for the establishment of crop plants. Several plant species identified as self-seeding live in partially processed piles of sulfuric acid heap-leaching uranium ores with a sufficient level of resistance to survive in soil with high levels of sulfate-containing salts and ionizing radiation of 1200–1400 μR/hr. These facts can authenticate to consider the possibility of growing these plant species in the artificial grassing of uranium-containing dumps to create herbage.
检查垃圾场、技术土壤和含有铀矿废物的地区的过度生长对于理解技术景观中植被覆盖的动态特征至关重要。以哈萨克斯坦山托别铀矿为例,研究了不同环境条件下堆积场和技术区内植物种类组成及其生产力的变化,以及土壤电离辐射强度的变化。垃圾场和技术基地的植被处于同生的早期阶段,是先驱者和群灌丛群落的代表。与强烈的硫酸盐盐碱化形成有关的不利生态条件在技术场所茁壮成长。然而,植物区系组成以高抗性种(Calamagrostis epigejos和Phragmites australis)和次生种为主。典型的哈萨克斯坦草原带植物群落的形成不会发生在这些地点。原铀矿复垦后的排土场和河岸的生产力相当高,为120-150克/平方米,与哈萨克斯坦北部的草甸-农村社区相匹配。而含铀矿石的非复耕和部分处理硫酸堆浸堆的生产率最低,为30 ~ 37 g/m2,这对作物植株的建立创造了极为不利的条件。在硫酸堆浸铀矿石部分处理过的堆堆中,几种自播植物具有足够的抗性,可以在高含硫酸盐水平和1200 ~ 1400 μR/hr电离辐射的土壤中生存。这些事实可以证明考虑在含铀堆积场人工草坪上种植这些植物以制造牧草的可能性。
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引用次数: 2
STATE AND PROSPECTS OF MINERAL FERTILIZERS USE IN AGRICULTURE OF KAZAKHSTAN 哈萨克斯坦矿质肥料在农业中的应用现状与展望
IF 1.4 Q3 PLANT SCIENCES Pub Date : 2023-06-30 DOI: 10.54910/sabrao2023.55.3.23
S. Kenenbaev, S. Ramazanova, VN Gusev
The article is a dedicated analysis of the production and use of mineral fertilizers globally, focusing on the agriculture of Kazakhstan. From 1950 to 2020, the fertilizer use ranged between 3.6–29.0 kg of NPK per hectare in arable agricultural land of Kazakhstan. The maximum benefit of fertilizers happened in 1986 in Kazakhstan, with 1,039 t of mineral fertilizers (active substances) applied at an area of 47% of the total arable land, while in 1965, the fertilized area was only 6.6% of the entire sown field. In Kazakhstan, the annual recommended need for mineral fertilizers is one million tons (active ingredient). The paper also discussed the influence of the long-term application of fertilizers on the leading indicators of soil fertility and crop productivity. Employing regression analysis also explored a high degree of probability of a positive relationship among the four viable factors, i.e., fertilizer use intensity, agrochemical indicators of the soil, crop plants yields, and their quality.
这篇文章专门分析了全球矿物肥料的生产和使用情况,重点是哈萨克斯坦的农业。1950年至2020年,哈萨克斯坦耕地氮磷钾用量为每公顷3.6-29.0 kg。1986年哈萨克斯坦的肥料效益达到最大,在占总耕地47%的土地上施用了1039吨矿物肥料(活性物质),而在1965年,施肥面积仅占整个播种面积的6.6%。在哈萨克斯坦,矿物肥料的年推荐需求量为100万吨(有效成分)。讨论了长期施用化肥对土壤肥力和作物生产力主要指标的影响。通过回归分析还发现了化肥使用强度、土壤农化指标、作物产量及其质量四个可行因素之间存在高度正相关的概率。
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引用次数: 3
PHYLOGENETIC ANALYSIS OF SOME SPECIES OF CYPERACEAE BASED ON DNA INTERNAL TRANSCRIBED SPACERS (ITS) IN IRAQ 基于DNA内转录间隔区(ITS)的伊拉克莎草科几种植物系统发育分析
IF 1.4 Q3 PLANT SCIENCES Pub Date : 2023-06-30 DOI: 10.54910/sabrao2023.55.3.9
B. Hamid, Zta AL-RUFAYE, Hala S. Khaleel, AR Abidukadhim
Cyperaceae is the third-largest monocot family and has ecological and commercial significance worldwide. It is also a perfect model family for evolutionary research due to species diversity and the wide range of variations in lineage diversity. Using a nuclear region internal transcribed spacer (ITS), the current study attempted to identify the three species (Carex otrubae, Bolboschoenus fluviatilis, and Eleocharis palustris) and examine their relationships within the Cyperaceae family. In 2021, gathering of samples transpired from various locations along the Al-Hussainiya River in Iraq and the National Herbarium. According to the findings, the length of the fragments sequenced ranged between 750 and 1000 bp. Results also showed the sequence alignments between the two species (Carex otrubae and Bolboschoenus fluviatilis) have similarities and differences with those deposited in the Genome Bank. However, the Eleocharis palustris showed complete similarity in all regions of the nitrogenous base sequences with species deposited in the Genome Bank.
莎草科是第三大单子叶植物科,在世界范围内具有重要的生态和商业意义。由于物种多样性和谱系多样性的广泛变化,它也是进化研究的完美模式家族。利用核区内部转录间隔区(ITS),目前的研究试图鉴定这三个物种(苔草、Bolboschoenus fluviatilis和Eleocharis palustris),并检查它们在莎草科中的关系。2021年,从伊拉克胡塞尼亚河和国家植物标本馆的不同地点采集了样本。根据研究结果,测序片段的长度在750到1000个碱基之间。结果还表明,这两个物种(苔草属和河苔草属)之间的序列比对与保存在基因组库中的序列比对有相似之处,也有不同之处。然而,沼泽Eleocharis在含氮碱基序列的所有区域都与基因组库中的物种表现出完全相似。
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引用次数: 0
GENETIC DIVERSITY OF DIFFERENT RADISH (RAPHANUS SATIVUS L.) CULTIVARS UNDER THE BASTAR PLATEAU OF CHHATTISGARH, INDIA 印度恰蒂斯加尔巴斯塔高原不同萝卜品种的遗传多样性
IF 1.4 Q3 PLANT SCIENCES Pub Date : 2023-06-30 DOI: 10.54910/sabrao2023.55.3.16
Nk Thakur, KP Singh, B. Singh, R. Shukla, Khemraj, P. Haldar
The presented study, held at the Research cum Instructional Farm of the College of Horticulture and Research Station, Jagdalpur, Chhattisgarh, India, ran during the Rabi season of 2021, to investigate superior radish cultivars for selection having high yield potential and better quality root for Bastar plateau. The experiment, laid out in randomized block design (RBD), had 15 treatments and three replications. Observing high genotypic and phenotypic coefficient of variation was for the following traits: dry and fresh weights of roots, plants, and leaves; root and leaf yields; days to 50% germination; the diameter and length of the root, North-South and East-West spread of the plant; plant height, and the number of leaf plant-1, having recorded significant heritability and high genetic advance. Similarly, high heritability showed for root yield and fresh and dry weights of roots. Moderate heritability levels appeared for the traits, viz., the plant’s dry weight and the root diameter. The genetic gain resulted high for characters, viz., root yield and fresh and dry weights of roots. High heritability followed by high genetic advance indicates that selection may be effective for improving such characteristics. Root yield (t ha-1) exhibited a highly significant positive correlation with the fresh weight of the plant at the phenotypic and genotypic levels, followed by dry weight of roots, dry weight of the plant, East-West spread of the plant, the diameter of the root, and leaf yield plot-1 . The fresh weight of roots had the maximum positive direct effect on root yield in the genotypic path, followed by the dry weight of roots and the plant, the East-West spread of the plant, the diameter of the root, and leaf yield in radish. The selection of these characters can benefit in improving the yield of radishes.
这项研究在印度恰蒂斯加尔邦贾格达尔布尔园艺学院和研究站的研究兼教学农场进行,于2021年拉比季节进行,旨在调查巴斯塔高原具有高产潜力和优质根系的优质萝卜品种。该实验采用随机区组设计(RBD),共有15次治疗和3次重复。观察到以下性状的高基因型和表型变异系数:根、植物和叶片的干重和鲜重;根和叶产量;天至50%发芽;根的直径和长度,植物的南北和东西分布;株高和叶株数-1,具有显著的遗传力和高的遗传进展。同样,根产量和根的鲜干重也表现出较高的遗传力。植株干重和根径等性状的遗传力水平适中。遗传增益对根系产量、根鲜重和干重等性状的影响较大。高遗传力和高遗传推进表明,选择可能对改善这些特性是有效的。根产量(t ha-1)在表型和基因型水平上与植物鲜重呈高度显著的正相关,其次是根干重、植物干重、植株东西向扩展、根直径和叶产量图-1。在基因型路径中,根的鲜重对根产量的直接影响最大,其次是根和植株的干重、植株的东西向扩展、根的直径和叶产量。这些性状的选择有利于提高萝卜产量。
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引用次数: 0
POLYMORPHISM AND INHERITANCE OF GLIADIN PROTEINS IN WHEAT LANDRACES OF UZBEKISTAN 乌兹别克斯坦小麦地方品种麦胶蛋白的多态性与遗传
IF 1.4 Q3 PLANT SCIENCES Pub Date : 2023-06-30 DOI: 10.54910/sabrao2023.55.3.6
A. Buronov, B. Amanov, K. Muminov, N. Tursunova, L. Umirova
Wheat genotypes grown in Uzbekistan and other Central Asian countries cannot compete with commercial cultivars for productivity. These landraces are valuable genetic resources that could benefit in developing new cultivars enriched with nutritious microelements and other ingredients beneficial for human health. As a result of wheat research, for the first time, preserving the 31 landraces in their natural form in the remote territories of Uzbekistan ensued, including a catalog of compilation by determining their economic and morphologically valuable features. Based on cluster analysis and quantitative characteristics, local wheat genotypes attained five groupings with a compiled dendrogram. With the dendrogram, determining the morphological attributes of ancient local cultivars of wheat materialized, even though the grain color is the same, a sharp difference is noticeable, but still similar based on quantitative characteristics that ensure the grain yield. In ancient local wheat cultivars, plant height and 1000-seed weight have a weak correlation with other traits, and a strong positive association was distinct between the spike length and spikelets per spike and the number and grain weight per spike. The polymorphism of soft wheat landraces showed according to the electrophoretic spectra in the grain’s gliadin proteins. The electrophoretic parameters of gliadin proteins indicated that of the six samples of cultivar Kzyl-bugdoy listed in the catalog, only four have similar electrophoretic spectra. In cultivar Ak-bugdoy’s four specimens, three have identical spectra, while three from the cultivar Surkhak have different bands. Based on individual selection and according to electrophoretic spectra from wheat landraces, the release of the wheat cultivar Kairaktash was due to its high baking and nutritional value.
在乌兹别克斯坦和其他中亚国家种植的小麦基因型在生产力方面无法与商业品种竞争。这些地方品种是宝贵的遗传资源,可用于开发富含营养微量元素和其他有益人类健康成分的新品种。作为小麦研究的结果,第一次在乌兹别克斯坦偏远地区以其自然形态保存了31个地方品种,包括通过确定其经济和形态价值特征而编制的目录。基于聚类分析和数量特征,在编制的树图中,小麦基因型可分为5类。通过树形图,确定古代地方小麦品种的形态属性成为现实,即使籽粒颜色相同,也有明显的差异,但基于保证籽粒产量的数量特征仍然相似。在古代地方小麦品种中,株高和千粒重与其他性状的相关性较弱,穗长和穗粒数与穗粒重的相关性较强。软质小麦地方品种籽粒麦胶蛋白的电泳图谱显示了籽粒麦胶蛋白的多态性。麦胶蛋白的电泳参数表明,在目录中所列的6个品种中,只有4个品种具有相似的电泳谱。在品种Ak-bugdoy的四个标本中,三个具有相同的光谱,而来自品种Surkhak的三个具有不同的光谱。根据小麦地方品种的个体选择和电泳谱分析,卡拉克塔什小麦品种的释放是由于其具有较高的烘焙和营养价值。
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引用次数: 2
QUALITATIVE CHARACTERISTICS AND GENOMIC ANALYSIS OF WHEAT GENOTYPES IN IRAQ 伊拉克小麦基因型的质量特征和基因组分析
IF 1.4 Q3 PLANT SCIENCES Pub Date : 2023-06-30 DOI: 10.54910/sabrao2023.55.3.24
E. Ismail, DM Majeed, HS Alagely, A. Abed, G. Hussein
The breeding material comprising 17 Iraqi wheat cultivars belongs to three different types, i.e., a) salinity-tolerant cultivars (Dajla, Furat, 1H, 2H, 2N, 3H, 3N, and 7H), b) drought-tolerant cultivars (Sham-6 and Orok), and c) local cultivars (Iraq, Iba99, Iba95, Abu Ghraib-3, Adnanin, Tamoze, and Alrashid) underwent qualitative characteristics and genomic analysis studies in 2021-2022, at the Biotechnology Research Center, Al-Nahrain University, Baghdad, Iraq. Measuring the percentage of protein, wet and dry gluten, and molecular fingerprinting used the randomly amplified polymorphic of DNA (RAPD) technique with six primers, with traits estimation using a dendrogram. The highest percentage of protein (24.5%), wet (52.7%), and dry gluten (27.3%) emerged from the wheat genotype Dajla. However, the recorded lowest percentages of wet (32.52%) and dry gluten (7.62%) appeared in wheat genotype Iba99. The cultivars Aadnania, Abu Ghraib-3, and Tamoze gave the lowest protein content of 9.45, 10.34, and 10.54, respectively. The cluster analysis divided 17 wheat genotypes into two large cluster groups. Amplification of all 365 loci used six primers. Fragments‟ size ranged from 100 bp to 2000 kb. The highest number of bands (73) was amplified with primer Pr-5, while the lowest number (48) was with primer Pr-1.
由17个伊拉克小麦品种组成的选育材料,分别属于3个不同类型,即a)耐盐品种(Dajla、Furat、1H、2H、2N、3H、3N和7H), b)耐旱品种(Sham-6和Orok),以及c)当地品种(Iraq、Iba99、Iba95、Abu Ghraib-3、Adnanin、Tamoze和Alrashid),于2021-2022年在伊拉克巴格达Al-Nahrain大学生物技术研究中心进行了定性特征和基因组分析研究。蛋白质、干湿面筋百分比测定和分子指纹鉴定采用6条引物随机扩增DNA多态性(RAPD)技术,并用树突图进行性状估计。Dajla基因型小麦蛋白含量最高(24.5%),湿蛋白含量最高(52.7%),干蛋白含量最高(27.3%)。而湿面筋和干面筋在Iba99基因型中所占比例最低,分别为32.52%和7.62%。Aadnania、Abu Ghraib-3和Tamoze的蛋白质含量最低,分别为9.45、10.34和10.54。聚类分析将17种小麦基因型划分为两大聚类群。所有365个位点使用6个引物扩增。片段的大小从100 bp到2000 kb不等。引物Pr-5扩增条带数最多(73条),引物Pr-1扩增条带数最少(48条)。
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引用次数: 1
USING SALT-TOLERANT RHIZOBIA TO IMPROVE THE SOYBEAN (GLYCINE MAX) RESILIENCE TO SALINITY 利用耐盐根瘤菌提高大豆(甘氨酸MAX)耐盐性
IF 1.4 Q3 PLANT SCIENCES Pub Date : 2023-06-30 DOI: 10.54910/sabrao2023.55.3.17
I. Smirnova, A. Sadanov, G. Baimakhanova, E. Faizulina, L. Tatarkina
Soybean (Glycine max [L.] Merr.) is an economically important oilseed crop with an annual increase in growing grain demand. Soybean is a moderately salt-tolerant crop; however, salt stress conditions can affect its growth and yield-related traits and, eventually, reduce productivity. In saline soils, one of the techniques to increase soybean productivity is to use rhizobia inoculation. Although, using industrial rhizobia-based biofertilizers is often ineffective due to their lack of adaptability to salinity. Injecting soybeans with salt-tolerant and growth-promoting rhizobia helps mitigate the effects of salt stress harmful to crop plants. The recent study sought to isolate local strains of salt-tolerant rhizobia, studying its ability to increase soybean tolerance to salt stress conditions. Twenty-four local salttolerant rhizobium isolates underwent isolation from root nodules of soybean grown on saline soils. Studying their basic morphological and biochemical characteristics and ability to withstand salt stress led to the final selection of five salt-tolerant strains. The rhizobium strains were able to synthesize metabolites that stimulate growth and help reduce salt stress in plants. The study of rhizobia nodulation ability under saline conditions resulted in selecting the three most efficient strains from the Bradyrhizobium japonicum species. Inoculation of soybean seeds with salt-tolerant rhizobia proved to mitigate the adverse effects of salinity on plant growth by increasing the root size and the number of nodules in the roots. Thus, the study establishes that inoculation of soybean seeds with local salttolerant rhizobia enhances soybean tolerance to salt stress and improves crop growth and adaptation to soil salinity. Using isolated local strains of salt-tolerant rhizobia will help provide a key and environmentally friendly approach to solving the problem of salt stress for sustainable agriculture.
大豆(Glycine max[L.]Merr.)是一种经济上重要的油料作物,其粮食需求每年都在增长。大豆是中等耐盐作物;然而,盐胁迫条件会影响其生长和产量相关性状,并最终降低生产力。在盐碱地,提高大豆产量的技术之一是接种根瘤菌。尽管如此,使用基于工业根瘤菌的生物肥料通常是无效的,因为它们对盐度缺乏适应性。给大豆注射耐盐和促进生长的根瘤菌有助于减轻对作物有害的盐胁迫影响。最近的研究试图分离出耐盐根瘤菌的本地菌株,研究其提高大豆对盐胁迫条件耐受性的能力。从盐碱地上生长的大豆根瘤中分离出24株当地耐盐根瘤菌。通过对其基本形态、生化特性和耐盐能力的研究,最终筛选出5个耐盐菌株。根瘤菌菌株能够合成刺激生长并有助于减少植物盐胁迫的代谢产物。通过对根瘤菌在生理盐水条件下结瘤能力的研究,从慢生根瘤菌中筛选出三个最有效的菌株。用耐盐根瘤菌接种大豆种子可以通过增加根系大小和根瘤数量来减轻盐度对植物生长的不利影响。因此,该研究表明,用当地耐盐根瘤菌接种大豆种子可以增强大豆对盐胁迫的耐受性,并改善作物生长和对土壤盐度的适应。使用当地分离的耐盐根瘤菌菌株将有助于为可持续农业解决盐胁迫问题提供一种关键且环保的方法。
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引用次数: 3
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Sabrao Journal of Breeding and Genetics
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