Staircases are critical escape routes in emergency evacuations. However, in-depth research on the mechanism by which “invisibility” alters our mind and body remains insufficient. This study quantifies individuals’ physiological and psychological states through staircase evacuation experiments comparing normal and complete invisibility scenarios, and integrates multimodal physiological monitoring (ECG, BVP, EDA, TEMP) with the PAD emotion scale for data collection. Innovatively, based on dynamic segmentation analysis of cardiac cycles at landing platforms, Principal Component Analysis (PCA) was employed to integrate multiple physiological indicators, resulting in the development of a high-precision quantitative model (R2 > 0.98). From the key findings of the experimental study, it can be drawn that under completely invisible conditions, the correlation between physiological indicators and cardiac cycles was significantly enhanced (correlation coefficients generally > 0.95). It has also been observed that the PCA-derived composite indicator F effectively characterized the evolution of physiological stress. Psychologically, completely invisible conditions led to reduced pleasure (P), increased arousal (A), and decreased dominance (D), with emotions deteriorating significantly from “anxiety” to “fear”. In contrast, under normal conditions, emotions remained relatively stable, exhibiting a “dependent”. The study revealed the neurobiological pathway of “environmental stimuli-physiological responses-emotional experiences”, where physiological stress drove emotional changes, while variations in emotional characteristics reciprocally validated physiological upheavals. Together, they formed a complete evidence chain for pedestrian stress responses under environmental pressure, providing critical quantitative foundations for risk assessment and emergency management.
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