Aly Makhlouf, Anfu Wang, Nanami Sato, Viviane S. Rosa, Marta N. Shahbazi
Primordial germ cells (PGCs) are the precursors of gametes and the sole mechanism by which animals transmit genetic information across generations. In the mouse embryo, the transcriptional and epigenetic regulation of PGC specification has been extensively characterized. However, the initial event that triggers the soma-germline segregation remains poorly understood. Here, we uncover a critical role for the basement membrane in regulating germline entry. We show that PGCs arise in a region of the mouse embryo that lacks contact with the basement membrane, and the addition of exogenous extracellular matrix (ECM) inhibits both PGC and PGC-like cell (PGCLC) specification in mouse embryos and stem cell models, respectively. Mechanistically, we demonstrate that the engagement of β1 integrin with laminin blocks PGCLC specification by preventing the Wnt signaling–dependent down-regulation of the PGC transcriptional repressor, Otx2. In this way, the physical segregation of cells away from the basement membrane acts as a morphogenetic fate switch that controls the soma-germline bifurcation.
{"title":"Integrin signaling in pluripotent cells acts as a gatekeeper of mouse germline entry","authors":"Aly Makhlouf, Anfu Wang, Nanami Sato, Viviane S. Rosa, Marta N. Shahbazi","doi":"10.1126/sciadv.adk2252","DOIUrl":"10.1126/sciadv.adk2252","url":null,"abstract":"<div >Primordial germ cells (PGCs) are the precursors of gametes and the sole mechanism by which animals transmit genetic information across generations. In the mouse embryo, the transcriptional and epigenetic regulation of PGC specification has been extensively characterized. However, the initial event that triggers the soma-germline segregation remains poorly understood. Here, we uncover a critical role for the basement membrane in regulating germline entry. We show that PGCs arise in a region of the mouse embryo that lacks contact with the basement membrane, and the addition of exogenous extracellular matrix (ECM) inhibits both PGC and PGC-like cell (PGCLC) specification in mouse embryos and stem cell models, respectively. Mechanistically, we demonstrate that the engagement of β1 integrin with laminin blocks PGCLC specification by preventing the Wnt signaling–dependent down-regulation of the PGC transcriptional repressor, Otx2. In this way, the physical segregation of cells away from the basement membrane acts as a morphogenetic fate switch that controls the soma-germline bifurcation.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":null,"pages":null},"PeriodicalIF":11.7,"publicationDate":"2024-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.science.org/doi/reader/10.1126/sciadv.adk2252","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142133657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Vanessa Mühlgrabner, Timo Peters, Rubí M.-H. Velasco Cárdenas, Benjamin Salzer, Janett Göhring, Angelika Plach, Maria Höhrhan, Iago Doel Perez, Vasco Dos Reis Goncalves, Jesús Siller Farfán, Manfred Lehner, Hannes Stockinger, Wolfgang W. Schamel, Kilian Schober, Dirk H. Busch, Michael Hudecek, Omer Dushek, Susana Minguet, René Platzer, Johannes B. Huppa
Low antigen sensitivity and a gradual loss of effector functions limit the clinical applicability of chimeric antigen receptor (CAR)–modified T cells and call for alternative antigen receptor designs for effective T cell–based cancer immunotherapy. Here, we applied advanced microscopy to demonstrate that TCR/CD3-based synthetic constructs (TCC) outperform second-generation CAR formats with regard to conveyed antigen sensitivities by up to a thousandfold. TCC-based antigen recognition occurred without adverse nonspecific signaling, which is typically observed in CAR–T cells, and did not depend—unlike sensitized peptide/MHC detection by conventional T cells—on CD4 or CD8 coreceptor engagement. TCC-endowed signaling properties may prove critical when targeting antigens in low abundance and aiming for a durable anticancer response.
低抗原敏感性和效应功能的逐渐丧失限制了嵌合抗原受体（CAR）修饰的T细胞的临床适用性，因此需要采用替代抗原受体设计来实现基于T细胞的有效癌症免疫疗法。在这里，我们应用先进的显微镜技术证明，基于 TCR/CD3 的合成构建体（TCC）在传递抗原敏感性方面比第二代 CAR 形式的抗原敏感性高出千倍。基于TCC的抗原识别不会出现CAR-T细胞通常会出现的不良非特异性信号传导，也不像传统T细胞那样依赖CD4或CD8核心受体的参与来检测敏化肽/MHC。当靶向低丰度抗原并寻求持久的抗癌反应时，TCC所具有的信号特性可能会被证明是至关重要的。
{"title":"TCR/CD3-based synthetic antigen receptors (TCC) convey superior antigen sensitivity combined with high fidelity of activation","authors":"Vanessa Mühlgrabner, Timo Peters, Rubí M.-H. Velasco Cárdenas, Benjamin Salzer, Janett Göhring, Angelika Plach, Maria Höhrhan, Iago Doel Perez, Vasco Dos Reis Goncalves, Jesús Siller Farfán, Manfred Lehner, Hannes Stockinger, Wolfgang W. Schamel, Kilian Schober, Dirk H. Busch, Michael Hudecek, Omer Dushek, Susana Minguet, René Platzer, Johannes B. Huppa","doi":"10.1126/sciadv.adj4632","DOIUrl":"10.1126/sciadv.adj4632","url":null,"abstract":"<div >Low antigen sensitivity and a gradual loss of effector functions limit the clinical applicability of chimeric antigen receptor (CAR)–modified T cells and call for alternative antigen receptor designs for effective T cell–based cancer immunotherapy. Here, we applied advanced microscopy to demonstrate that TCR/CD3-based synthetic constructs (TCC) outperform second-generation CAR formats with regard to conveyed antigen sensitivities by up to a thousandfold. TCC-based antigen recognition occurred without adverse nonspecific signaling, which is typically observed in CAR–T cells, and did not depend—unlike sensitized peptide/MHC detection by conventional T cells—on CD4 or CD8 coreceptor engagement. TCC-endowed signaling properties may prove critical when targeting antigens in low abundance and aiming for a durable anticancer response.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":null,"pages":null},"PeriodicalIF":11.7,"publicationDate":"2024-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.science.org/doi/reader/10.1126/sciadv.adj4632","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142133665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Catastrophic sediment overloading of mountain streams in response to coseismic landsliding causes river systems to fundamentally reorganize their morphology and sediment transporting characteristics, influencing sediment yields, bedrock incision, and the coupling between erosion and tectonics. A sequence of 13 airborne LiDAR surveys of an alpine tributary of the Hāpuku River, New Zealand, reveals patterns of sediment mass balance change over 5 years following delivery of 6.6 million cubic meters of landslide debris during the 2016 magnitude 7.8 Kaikōura earthquake. The surveys reveal how mountain river systems modulate catastrophic sediment deliveries to their lower reaches through sediment storage, evolution of channel morphology, and armoring of the bed. Variations in valley width contribute to the delay and diffusion of the seismically induced disturbance “wave” as it moves across river process domains. The landslide sediment train remnants may persist for longer than the return time of their triggering mechanism, leading to a long-lived hiatus in bedrock incision in this tectonically active mountain catchment.
{"title":"Controls on fluvial sediment evacuation following an earthquake-triggered landslide: Observations from LiDAR time series","authors":"Jon Tunnicliffe, Jamie Howarth, Chris Massey","doi":"10.1126/sciadv.adi5560","DOIUrl":"10.1126/sciadv.adi5560","url":null,"abstract":"<div >Catastrophic sediment overloading of mountain streams in response to coseismic landsliding causes river systems to fundamentally reorganize their morphology and sediment transporting characteristics, influencing sediment yields, bedrock incision, and the coupling between erosion and tectonics. A sequence of 13 airborne LiDAR surveys of an alpine tributary of the Hāpuku River, New Zealand, reveals patterns of sediment mass balance change over 5 years following delivery of 6.6 million cubic meters of landslide debris during the 2016 magnitude 7.8 Kaikōura earthquake. The surveys reveal how mountain river systems modulate catastrophic sediment deliveries to their lower reaches through sediment storage, evolution of channel morphology, and armoring of the bed. Variations in valley width contribute to the delay and diffusion of the seismically induced disturbance “wave” as it moves across river process domains. The landslide sediment train remnants may persist for longer than the return time of their triggering mechanism, leading to a long-lived hiatus in bedrock incision in this tectonically active mountain catchment.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":null,"pages":null},"PeriodicalIF":11.7,"publicationDate":"2024-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.science.org/doi/reader/10.1126/sciadv.adi5560","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142133650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Qianyu Hu, Liang Huang, Yaoyu Yang, Ye Xiang, Jintao Liu
Despite extensive knowledge on phage resistance at bacterium level, the resistance of bacterial communities is still not well-understood. Given its ubiquity, it is essential to understand resistance at the community level. We performed quantitative investigations on the dynamics of phage infection in Klebsiella pneumoniae biofilms. We found that the biofilms quickly developed resistance and resumed growth. Instead of mutations, the resistance was caused by unassembled phage tail fibers released by the phage-lysed bacteria. The tail fibers degraded the bacterial capsule essential for infection and induced spreading of capsule loss in the biofilm, and tuning tail fiber and capsule levels altered the resistance. Latent infections sustained in the biofilm despite resistance, allowing stable phage-bacteria coexistence. Last, we showed that the resistance exposed vulnerabilities in the biofilm. Our findings indicate that phage lysate plays important roles in shaping phage-biofilm interactions and open more dimensions for the rational design of strategies to counter bacteria with phage.
{"title":"Essential phage component induces resistance of bacterial community","authors":"Qianyu Hu, Liang Huang, Yaoyu Yang, Ye Xiang, Jintao Liu","doi":"10.1126/sciadv.adp5057","DOIUrl":"10.1126/sciadv.adp5057","url":null,"abstract":"<div >Despite extensive knowledge on phage resistance at bacterium level, the resistance of bacterial communities is still not well-understood. Given its ubiquity, it is essential to understand resistance at the community level. We performed quantitative investigations on the dynamics of phage infection in <i>Klebsiella pneumoniae</i> biofilms. We found that the biofilms quickly developed resistance and resumed growth. Instead of mutations, the resistance was caused by unassembled phage tail fibers released by the phage-lysed bacteria. The tail fibers degraded the bacterial capsule essential for infection and induced spreading of capsule loss in the biofilm, and tuning tail fiber and capsule levels altered the resistance. Latent infections sustained in the biofilm despite resistance, allowing stable phage-bacteria coexistence. Last, we showed that the resistance exposed vulnerabilities in the biofilm. Our findings indicate that phage lysate plays important roles in shaping phage-biofilm interactions and open more dimensions for the rational design of strategies to counter bacteria with phage.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":null,"pages":null},"PeriodicalIF":11.7,"publicationDate":"2024-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.science.org/doi/reader/10.1126/sciadv.adp5057","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142133653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Désirée Jacqueline Wendering, Leila Amini, Stephan Schlickeiser, Martí Farrera-Sal, Sarah Schulenberg, Lena Peter, Marco Mai, Tino Vollmer, Weijie Du, Maik Stein, Frederik Hamm, Alisier Malard, Carla Castro, Mingxing Yang, Ramon Ranka, Timo Rückert, Pawel Durek, Frederik Heinrich, Gilles Gasparoni, Abdulrahman Salhab, Jörn Walter, Dimitrios Laurin Wagner, Mir-Farzin Mashreghi, Sybille Landwehr-Kenzel, Julia K. Polansky, Petra Reinke, Hans-Dieter Volk, Michael Schmueck-Henneresse
Regulatory T cells (Treg cells) hold promise for sustainable therapy of immune disorders. Recent advancements in chimeric antigen receptor development and genome editing aim to enhance the specificity and function of Treg cells. However, impurities and functional instability pose challenges for the development of safe gene-edited Treg cell products. Here, we examined different Treg cell subsets regarding their fate, epigenomic stability, transcriptomes, T cell receptor repertoires, and function ex vivo and after manufacturing. Each Treg cell subset displayed distinct features, including lineage stability, epigenomics, surface markers, T cell receptor diversity, and transcriptomics. Earlier-differentiated memory Treg cell populations, including a hitherto unidentified naïve-like memory Treg cell subset, outperformed late-differentiated effector memory–like Treg cells in regulatory function, proliferative capacity, and epigenomic stability. High yields of stable, functional Treg cell products could be achieved by depleting the small effector memory–like Treg cell subset before manufacturing. Considering Treg cell subset composition appears critical to maintain lineage stability in the final cell product.
{"title":"Effector memory–type regulatory T cells display phenotypic and functional instability","authors":"Désirée Jacqueline Wendering, Leila Amini, Stephan Schlickeiser, Martí Farrera-Sal, Sarah Schulenberg, Lena Peter, Marco Mai, Tino Vollmer, Weijie Du, Maik Stein, Frederik Hamm, Alisier Malard, Carla Castro, Mingxing Yang, Ramon Ranka, Timo Rückert, Pawel Durek, Frederik Heinrich, Gilles Gasparoni, Abdulrahman Salhab, Jörn Walter, Dimitrios Laurin Wagner, Mir-Farzin Mashreghi, Sybille Landwehr-Kenzel, Julia K. Polansky, Petra Reinke, Hans-Dieter Volk, Michael Schmueck-Henneresse","doi":"10.1126/sciadv.adn3470","DOIUrl":"10.1126/sciadv.adn3470","url":null,"abstract":"<div >Regulatory T cells (T<sub>reg</sub> cells) hold promise for sustainable therapy of immune disorders. Recent advancements in chimeric antigen receptor development and genome editing aim to enhance the specificity and function of T<sub>reg</sub> cells. However, impurities and functional instability pose challenges for the development of safe gene-edited T<sub>reg</sub> cell products. Here, we examined different T<sub>reg</sub> cell subsets regarding their fate, epigenomic stability, transcriptomes, T cell receptor repertoires, and function ex vivo and after manufacturing. Each T<sub>reg</sub> cell subset displayed distinct features, including lineage stability, epigenomics, surface markers, T cell receptor diversity, and transcriptomics. Earlier-differentiated memory T<sub>reg</sub> cell populations, including a hitherto unidentified naïve-like memory T<sub>reg</sub> cell subset, outperformed late-differentiated effector memory–like T<sub>reg</sub> cells in regulatory function, proliferative capacity, and epigenomic stability. High yields of stable, functional T<sub>reg</sub> cell products could be achieved by depleting the small effector memory–like T<sub>reg</sub> cell subset before manufacturing. Considering T<sub>reg</sub> cell subset composition appears critical to maintain lineage stability in the final cell product.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":null,"pages":null},"PeriodicalIF":11.7,"publicationDate":"2024-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.science.org/doi/reader/10.1126/sciadv.adn3470","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142133651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ottman A. Tertuliano, Philip J. DePond, Andrew C. Lee, Jiho Hong, David Doan, Luc Capaldi, Mark Brongersma, X. Wendy Gu, Manyalibo J. Matthews, Wei Cai, Adrian J. Lew
The widespread application of metal additive manufacturing (AM) is limited by the ability to control the complex interactions between the energy source and the feedstock material. Here, we develop a generalizable process to introduce nanoscale grooves to the surface of metal powders which increases the powder absorptivity by up to 70% during laser powder bed fusion. Absorptivity enhancements in copper, copper-silver, and tungsten enable energy-efficient manufacturing, with printing of pure copper at relative densities up to 92% using laser energy densities as low as 83 joules per cubic millimeter. Simulations show that the enhanced powder absorptivity results from plasmon-enabled light concentration in nanoscale grooves combined with multiple scattering events. The approach taken here demonstrates a general method to enhance the absorptivity and printability of reflective and refractory metal powders by changing the surface morphology of the feedstock without altering its composition.
{"title":"High absorptivity nanotextured powders for additive manufacturing","authors":"Ottman A. Tertuliano, Philip J. DePond, Andrew C. Lee, Jiho Hong, David Doan, Luc Capaldi, Mark Brongersma, X. Wendy Gu, Manyalibo J. Matthews, Wei Cai, Adrian J. Lew","doi":"10.1126/sciadv.adp0003","DOIUrl":"10.1126/sciadv.adp0003","url":null,"abstract":"<div >The widespread application of metal additive manufacturing (AM) is limited by the ability to control the complex interactions between the energy source and the feedstock material. Here, we develop a generalizable process to introduce nanoscale grooves to the surface of metal powders which increases the powder absorptivity by up to 70% during laser powder bed fusion. Absorptivity enhancements in copper, copper-silver, and tungsten enable energy-efficient manufacturing, with printing of pure copper at relative densities up to 92% using laser energy densities as low as 83 joules per cubic millimeter. Simulations show that the enhanced powder absorptivity results from plasmon-enabled light concentration in nanoscale grooves combined with multiple scattering events. The approach taken here demonstrates a general method to enhance the absorptivity and printability of reflective and refractory metal powders by changing the surface morphology of the feedstock without altering its composition.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":null,"pages":null},"PeriodicalIF":11.7,"publicationDate":"2024-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.science.org/doi/reader/10.1126/sciadv.adp0003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142133654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Uluk Rasulov, Harrison K. Wang, Thibault Viennet, Maxim A. Droemer, Srđan Matosin, Sebastian Schindler, Zhen-Yu J. Sun, Luca Mureddu, Geerten W. Vuister, Scott A. Robson, Haribabu Arthanari, Ilya Kuprov
The current standard method for amino acid signal identification in protein NMR spectra is sequential assignment using triple-resonance experiments. Good software and elaborate heuristics exist, but the process remains laboriously manual. Machine learning does help, but its training databases need millions of samples that cover all relevant physics and every kind of instrumental artifact. In this communication, we offer a solution to this problem. We propose polyadic decompositions to store millions of simulated three-dimensional NMR spectra, on-the-fly generation of artifacts during training, a probabilistic way to incorporate prior and posterior information, and integration with the industry standard CcpNmr software framework. The resulting neural nets take [1H,13C] slices of mixed pyruvate–labeled HNCA spectra (different CA signal shapes for different residue types) and return an amino acid probability table. In combination with primary sequence information, backbones of common proteins (GB1, MBP, and INMT) are rapidly assigned from just the HNCA spectrum.
{"title":"Protein NMR assignment by isotope pattern recognition","authors":"Uluk Rasulov, Harrison K. Wang, Thibault Viennet, Maxim A. Droemer, Srđan Matosin, Sebastian Schindler, Zhen-Yu J. Sun, Luca Mureddu, Geerten W. Vuister, Scott A. Robson, Haribabu Arthanari, Ilya Kuprov","doi":"10.1126/sciadv.ado0403","DOIUrl":"10.1126/sciadv.ado0403","url":null,"abstract":"<div >The current standard method for amino acid signal identification in protein NMR spectra is sequential assignment using triple-resonance experiments. Good software and elaborate heuristics exist, but the process remains laboriously manual. Machine learning does help, but its training databases need millions of samples that cover all relevant physics and every kind of instrumental artifact. In this communication, we offer a solution to this problem. We propose polyadic decompositions to store millions of simulated three-dimensional NMR spectra, on-the-fly generation of artifacts during training, a probabilistic way to incorporate prior and posterior information, and integration with the industry standard CcpNmr software framework. The resulting neural nets take [<sup>1</sup>H,<sup>13</sup>C] slices of mixed pyruvate–labeled HNCA spectra (different CA signal shapes for different residue types) and return an amino acid probability table. In combination with primary sequence information, backbones of common proteins (GB1, MBP, and INMT) are rapidly assigned from just the HNCA spectrum.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":null,"pages":null},"PeriodicalIF":11.7,"publicationDate":"2024-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.science.org/doi/reader/10.1126/sciadv.ado0403","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142133661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mengjun Wang, Jun Jia, Zhaodong Meng, Jing Xia, Xinyan Hu, Fei Xue, Huiping Peng, Xiangmin Meng, Jun Yi, Xiaolan Chen, Jun Li, Yuzheng Guo, Yong Xu, Xiaoqing Huang
Photothermal catalysis effectively increases catalytic activity by using the photothermal effect of metal nanomaterials; however, the combination of strong light absorption and high catalytic performance remains a challenge. Here, we demonstrate hexagonal ~5-nanometer-thick palladium antimony (chemical formula as Pd8Sb3) nanosheets (NSs) that exhibit strong light absorption within full spectral and localized surface plasmon resonance (LSPR) effects in the visible region. Such LSPR features lead to strong photothermal effects, and Pd8Sb3 NSs aqueous dispersion enables enhanced photothermal methane (CH4) conversion to formaldehyde (HCHO) under full-spectrum light irradiation at 1.7 watts per square centimeter, leading to selectivity of ~98.7%, productivity of ~665 millimoles per gram of catalyst, ~700 times higher than that of Pd NSs. Mechanism investigations suggest that different radicals were generated on Pd8Sb3 (·OH) and Pd NSs (·O2−), where Pd8Sb3 NSs displays stronger adsorption strength to CH4 and facilitates CH4 oxidation to HCHO. Besides, the strong light absorption ability of Pd8Sb3 NSs enables photothermal therapy for breast cancer.
{"title":"Plasmonic Pd-Sb nanosheets for photothermal CH4 conversion to HCHO and therapy","authors":"Mengjun Wang, Jun Jia, Zhaodong Meng, Jing Xia, Xinyan Hu, Fei Xue, Huiping Peng, Xiangmin Meng, Jun Yi, Xiaolan Chen, Jun Li, Yuzheng Guo, Yong Xu, Xiaoqing Huang","doi":"10.1126/sciadv.ado9664","DOIUrl":"10.1126/sciadv.ado9664","url":null,"abstract":"<div >Photothermal catalysis effectively increases catalytic activity by using the photothermal effect of metal nanomaterials; however, the combination of strong light absorption and high catalytic performance remains a challenge. Here, we demonstrate hexagonal ~5-nanometer-thick palladium antimony (chemical formula as Pd<sub>8</sub>Sb<sub>3</sub>) nanosheets (NSs) that exhibit strong light absorption within full spectral and localized surface plasmon resonance (LSPR) effects in the visible region. Such LSPR features lead to strong photothermal effects, and Pd<sub>8</sub>Sb<sub>3</sub> NSs aqueous dispersion enables enhanced photothermal methane (CH<sub>4</sub>) conversion to formaldehyde (HCHO) under full-spectrum light irradiation at 1.7 watts per square centimeter, leading to selectivity of ~98.7%, productivity of ~665 millimoles per gram of catalyst, ~700 times higher than that of Pd NSs. Mechanism investigations suggest that different radicals were generated on Pd<sub>8</sub>Sb<sub>3</sub> (·OH) and Pd NSs (·O<sub>2</sub><sup>−</sup>), where Pd<sub>8</sub>Sb<sub>3</sub> NSs displays stronger adsorption strength to CH<sub>4</sub> and facilitates CH<sub>4</sub> oxidation to HCHO. Besides, the strong light absorption ability of Pd<sub>8</sub>Sb<sub>3</sub> NSs enables photothermal therapy for breast cancer.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":null,"pages":null},"PeriodicalIF":11.7,"publicationDate":"2024-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.science.org/doi/reader/10.1126/sciadv.ado9664","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142133660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tae-Min Jang, Won Bae Han, Seungkeun Han, Ankan Dutta, Jun Hyeon Lim, Taekyung Kim, Bong Hee Lim, Gwan-Jin Ko, Jeong-Woong Shin, Rajaram Kaveti, Heeseok Kang, Chan-Hwi Eom, So Jeong Choi, Amay J. Bandodkar, Kyu-Sung Lee, Eunkyoung Park, Huanyu Cheng, Woon-Hong Yeo, Suk-Won Hwang
As the regenerative mechanisms of biological organisms, self-healing provides useful functions for soft electronics or associated systems. However, there have been few examples of soft electronics where all components have self-healing properties while also ensuring compatibility between components to achieve multifunctional and resilient bio-integrated electronics. Here, we introduce a stretchable, biodegradable, self-healing conductor constructed by combination of two layers: (i) synthetic self-healing elastomer and (ii) self-healing conductive composite with additives. Abundant dynamic disulfide and hydrogen bonds of the elastomer and conductive composite enable rapid and complete recovery of electrical conductivity (~1000 siemens per centimeter) and stretchability (~500%) in response to repetitive damages, and chemical interactions of interpenetrated polymer chains of these components facilitate robust adhesion strength, even under extreme mechanical stress. System-level demonstration of soft, self-healing electronics with diagnostic/therapeutic functions for the urinary bladder validates the possibility for versatile, practical uses in biomedical research areas.
{"title":"Stretchable and biodegradable self-healing conductors for multifunctional electronics","authors":"Tae-Min Jang, Won Bae Han, Seungkeun Han, Ankan Dutta, Jun Hyeon Lim, Taekyung Kim, Bong Hee Lim, Gwan-Jin Ko, Jeong-Woong Shin, Rajaram Kaveti, Heeseok Kang, Chan-Hwi Eom, So Jeong Choi, Amay J. Bandodkar, Kyu-Sung Lee, Eunkyoung Park, Huanyu Cheng, Woon-Hong Yeo, Suk-Won Hwang","doi":"10.1126/sciadv.adp9818","DOIUrl":"10.1126/sciadv.adp9818","url":null,"abstract":"<div >As the regenerative mechanisms of biological organisms, self-healing provides useful functions for soft electronics or associated systems. However, there have been few examples of soft electronics where all components have self-healing properties while also ensuring compatibility between components to achieve multifunctional and resilient bio-integrated electronics. Here, we introduce a stretchable, biodegradable, self-healing conductor constructed by combination of two layers: (i) synthetic self-healing elastomer and (ii) self-healing conductive composite with additives. Abundant dynamic disulfide and hydrogen bonds of the elastomer and conductive composite enable rapid and complete recovery of electrical conductivity (~1000 siemens per centimeter) and stretchability (~500%) in response to repetitive damages, and chemical interactions of interpenetrated polymer chains of these components facilitate robust adhesion strength, even under extreme mechanical stress. System-level demonstration of soft, self-healing electronics with diagnostic/therapeutic functions for the urinary bladder validates the possibility for versatile, practical uses in biomedical research areas.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":null,"pages":null},"PeriodicalIF":11.7,"publicationDate":"2024-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.science.org/doi/reader/10.1126/sciadv.adp9818","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142133664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aritra Biswas, Sang Lee, Pablo Cencillo-Abad, Manobina Karmakar, Jay Patel, Mahdi Soudi, Debashis Chanda
Neurotransmitters are crucial for the proper functioning of neural systems, with dopamine playing a pivotal role in cognition, emotions, and motor control. Dysregulated dopamine levels are linked to various disorders, underscoring the need for accurate detection in research and diagnostics. Single-stranded DNA (ssDNA) aptamers are promising bioreceptors for dopamine detection due to their selectivity, improved stability, and synthesis feasibility. However, discrepancies in dopamine specificity have presented challenges. Here, we surface-functionalized a nano-plasmonic biosensing platform with a dopamine-specific ssDNA aptamer for selective detection. The biosensor, featuring narrowband hybrid plasmonic resonances, achieves high specificity through functionalization with aptamers and passivation processes. Sensitivity and selectivity for dopamine detection are demonstrated across a wide range of concentrations, including in diverse biological samples like protein solutions, cerebrospinal fluid, and whole blood. These results highlight the potential of plasmonic “aptasensors” for developing rapid and accurate diagnostic tools for disease monitoring, medical diagnostics, and targeted therapies.
{"title":"Nanoplasmonic aptasensor for sensitive, selective, and real-time detection of dopamine from unprocessed whole blood","authors":"Aritra Biswas, Sang Lee, Pablo Cencillo-Abad, Manobina Karmakar, Jay Patel, Mahdi Soudi, Debashis Chanda","doi":"10.1126/sciadv.adp7460","DOIUrl":"10.1126/sciadv.adp7460","url":null,"abstract":"<div >Neurotransmitters are crucial for the proper functioning of neural systems, with dopamine playing a pivotal role in cognition, emotions, and motor control. Dysregulated dopamine levels are linked to various disorders, underscoring the need for accurate detection in research and diagnostics. Single-stranded DNA (ssDNA) aptamers are promising bioreceptors for dopamine detection due to their selectivity, improved stability, and synthesis feasibility. However, discrepancies in dopamine specificity have presented challenges. Here, we surface-functionalized a nano-plasmonic biosensing platform with a dopamine-specific ssDNA aptamer for selective detection. The biosensor, featuring narrowband hybrid plasmonic resonances, achieves high specificity through functionalization with aptamers and passivation processes. Sensitivity and selectivity for dopamine detection are demonstrated across a wide range of concentrations, including in diverse biological samples like protein solutions, cerebrospinal fluid, and whole blood. These results highlight the potential of plasmonic “aptasensors” for developing rapid and accurate diagnostic tools for disease monitoring, medical diagnostics, and targeted therapies.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":null,"pages":null},"PeriodicalIF":11.7,"publicationDate":"2024-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.science.org/doi/reader/10.1126/sciadv.adp7460","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142133658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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