Pub Date : 2014-04-13DOI: 10.1109/NEMS.2014.6908860
Chia-Hui Li, L. Chieng, Irrigepalli Srinivasa Thimmaraju, D. Yao
An analytical methodology using Thermal Desorption (TD) and GC-MS was developed for the determination of components in coffee beans aromas. The purpose was to estimate the volatile compounds/components in coffee beans aromas of different roast levels, such as light, medium, medium-dark, and dark roast. Aromas samples were collected by using stainless steel tubes containing approximately 200 mg of Tenax TA adsorbents and sampling at a flow rate of 200 mL/min. Seventeen major components in coffee beans aromas were identified by the TD-GC-MS system. Results showed that most components increased with roast levels. However, furfurals had a different downward trend. Chemical reactions occurred during the roasting process were proposed to explain the decrease in furfurals: furfurals changed to 2-furanmethanol and 2-methylfuran.
{"title":"Using TD-GC-MS to analyze coffee beans aromas of different roast levels","authors":"Chia-Hui Li, L. Chieng, Irrigepalli Srinivasa Thimmaraju, D. Yao","doi":"10.1109/NEMS.2014.6908860","DOIUrl":"https://doi.org/10.1109/NEMS.2014.6908860","url":null,"abstract":"An analytical methodology using Thermal Desorption (TD) and GC-MS was developed for the determination of components in coffee beans aromas. The purpose was to estimate the volatile compounds/components in coffee beans aromas of different roast levels, such as light, medium, medium-dark, and dark roast. Aromas samples were collected by using stainless steel tubes containing approximately 200 mg of Tenax TA adsorbents and sampling at a flow rate of 200 mL/min. Seventeen major components in coffee beans aromas were identified by the TD-GC-MS system. Results showed that most components increased with roast levels. However, furfurals had a different downward trend. Chemical reactions occurred during the roasting process were proposed to explain the decrease in furfurals: furfurals changed to 2-furanmethanol and 2-methylfuran.","PeriodicalId":22566,"journal":{"name":"The 9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS)","volume":"35 1","pages":"507-512"},"PeriodicalIF":0.0,"publicationDate":"2014-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84924848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2014-04-13DOI: 10.1109/NEMS.2014.6908880
S. A. M. Lajimi, G. Heppler, E. Abdel-Rahman
For a new type of a MEMS gyroscope made of a cantilever beam and an end-rigid-body, the reduced-order (discretized) model is obtained by using the method of assumed modes. The discretized model's static behavior is verified by comparing with the stationary partial differential governing equations. The free dynamics of the system are studied under a constant input voltage. The gyroscopic natural and pseudo-natural frequencies of the system are obtained. To operate the gyroscope in the frequency-modulation mode, the input angular rate is computed in analytical and numerical form.
{"title":"The application of a new beam-rigid body MEMS gyroscope in the frequency-modulation mode","authors":"S. A. M. Lajimi, G. Heppler, E. Abdel-Rahman","doi":"10.1109/NEMS.2014.6908880","DOIUrl":"https://doi.org/10.1109/NEMS.2014.6908880","url":null,"abstract":"For a new type of a MEMS gyroscope made of a cantilever beam and an end-rigid-body, the reduced-order (discretized) model is obtained by using the method of assumed modes. The discretized model's static behavior is verified by comparing with the stationary partial differential governing equations. The free dynamics of the system are studied under a constant input voltage. The gyroscopic natural and pseudo-natural frequencies of the system are obtained. To operate the gyroscope in the frequency-modulation mode, the input angular rate is computed in analytical and numerical form.","PeriodicalId":22566,"journal":{"name":"The 9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS)","volume":"47 1","pages":"586-591"},"PeriodicalIF":0.0,"publicationDate":"2014-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80872388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2014-04-13DOI: 10.1109/NEMS.2014.6908895
K. Chang, Jing-Lun Li, Ching-Hsuan Yang, S. Shiesh, Gwo-Bin Lee
Measurement of the ratio between glycated hemoglobin (HbA1c) and total hemoglobin (Hb) is now recommended for screening and diagnosing diabetes, in addition to monitoring long-term glycemic control for patient management. The costly and time-consuming traditional assay methods, which involve multi-step, bench-top, and antibody-based immunoassays, have presented major challenges to their clinical utility. In this study, an integrated microfluidic system capable of rapidly measuring the level of HbA1c and Hb in an automated fashion was reported. Moreover, this detection assay used nucleic acid aptamers that exhibited high sensitivity and specificity to Hb and HbA1c to further enhance the robustness of the assay. Combining the advantages of microfluidics and aptamers, this system presents a promising tool for clinical diagnosis and management of diabetes. As a demonstration of its clinical utility, we analyzed patient whole blood samples with clinically-relevant concentrations of HbA1c and Hb (4.4%-12.5% and 12.1 g/dL-14.9 g/dL, respectively) on our integrated microfluidic system.
{"title":"Measurement of glycated hemoglobin using an aptamer/antibody assay on an integrated microfluidic system","authors":"K. Chang, Jing-Lun Li, Ching-Hsuan Yang, S. Shiesh, Gwo-Bin Lee","doi":"10.1109/NEMS.2014.6908895","DOIUrl":"https://doi.org/10.1109/NEMS.2014.6908895","url":null,"abstract":"Measurement of the ratio between glycated hemoglobin (HbA1c) and total hemoglobin (Hb) is now recommended for screening and diagnosing diabetes, in addition to monitoring long-term glycemic control for patient management. The costly and time-consuming traditional assay methods, which involve multi-step, bench-top, and antibody-based immunoassays, have presented major challenges to their clinical utility. In this study, an integrated microfluidic system capable of rapidly measuring the level of HbA1c and Hb in an automated fashion was reported. Moreover, this detection assay used nucleic acid aptamers that exhibited high sensitivity and specificity to Hb and HbA1c to further enhance the robustness of the assay. Combining the advantages of microfluidics and aptamers, this system presents a promising tool for clinical diagnosis and management of diabetes. As a demonstration of its clinical utility, we analyzed patient whole blood samples with clinically-relevant concentrations of HbA1c and Hb (4.4%-12.5% and 12.1 g/dL-14.9 g/dL, respectively) on our integrated microfluidic system.","PeriodicalId":22566,"journal":{"name":"The 9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS)","volume":"31 1","pages":"647-650"},"PeriodicalIF":0.0,"publicationDate":"2014-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87112241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2014-04-13DOI: 10.1109/NEMS.2014.6908792
Long Wang, Qiancheng Zhao, Minghao Nie, Dachuan Liu, Yuxian Liu, Junjie Yan, Zhenchuan Yang, G. Yan
In this paper, we present an optimized digital fuzzy control strategy in order to realize fast startup of MEMS gyroscopes in various ambient temperatures. In the proposed procedure, the MEMS (Micro-ElectroMechanical Systems) vibratory gyroscope is driven with a sinusoidal wave synthesizer of adjustable frequency based on CORDIC algorithm. The drive frequency is fixed on the resonant frequency of the gyroscope with a frequency controller. By deliberate design of startup frequency controller using fuzzy control strategy, the startup time is decreased effectively in different ambient temperatures verified with test results. A series of startup test is performed under ambient temperatures ranging from -30 to 60°C, the tested startup time is improved from ~2.5s (tested with PID frequency controller) to ~100ms. What's more, this procedure has a higher SNR of 88dB.
{"title":"Digital fast startup procedure for micro-machined vibratory gyroscopes using optimized fuzzy control strategy","authors":"Long Wang, Qiancheng Zhao, Minghao Nie, Dachuan Liu, Yuxian Liu, Junjie Yan, Zhenchuan Yang, G. Yan","doi":"10.1109/NEMS.2014.6908792","DOIUrl":"https://doi.org/10.1109/NEMS.2014.6908792","url":null,"abstract":"In this paper, we present an optimized digital fuzzy control strategy in order to realize fast startup of MEMS gyroscopes in various ambient temperatures. In the proposed procedure, the MEMS (Micro-ElectroMechanical Systems) vibratory gyroscope is driven with a sinusoidal wave synthesizer of adjustable frequency based on CORDIC algorithm. The drive frequency is fixed on the resonant frequency of the gyroscope with a frequency controller. By deliberate design of startup frequency controller using fuzzy control strategy, the startup time is decreased effectively in different ambient temperatures verified with test results. A series of startup test is performed under ambient temperatures ranging from -30 to 60°C, the tested startup time is improved from ~2.5s (tested with PID frequency controller) to ~100ms. What's more, this procedure has a higher SNR of 88dB.","PeriodicalId":22566,"journal":{"name":"The 9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS)","volume":"13 1","pages":"208-211"},"PeriodicalIF":0.0,"publicationDate":"2014-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88463436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2014-04-13DOI: 10.1109/NEMS.2014.6908889
C. Chuang, Jing-Wei Ju, Kai-Chieh Chang, Yao-Wei Huang, C. Jen
This paper develops a multi-antibody array biochip for detection of different-grade bladder cancer cell lysates based on protein expression level. Two nanoprobes modified with antibodies, Galectin-1 (Gal-1) and Lactate Dehydrogenase B (LDH-B), were immobilized in an array manner by sequent DEP manipulation steps including focusing, guiding and trapping, therefore, two different protein levels can be achieved in single chip without complex microchannel design. The structure of DEP chip consists of three electrode layers, top electrode made of Indium Tin Oxide (ITO), middle Au electrode on SU-8 surface for focusing and guiding of nanoprobes, and bottom ITO dot electrodes under SU-8 cavity for trapping of nanoprobes. In our experiments, two different grade bladder cancer cell lysates (grade I:RT4 and grade III:T24) were introduced into immunosensor, the expression levels of Gal-1 and LDH-B displayed different fluorescent responses for RT4 and T24 cell lysates. Accordingly, the fluorescence intensity of Gal-1 is higher than the value of LDH-B for T24 cell lysate; however, the response for RT4 is opposite, i.e. LDH-B > Gal-1. Consequently, determining of different-grade bladder cancer cell can be achieved by using present multi-antibody immunosensor. In addition, the DEP manipulations of nanoprobes also demonstrate the feasibility for multi-biomarker array in single chip for point-of-care diagnostics.
本文研制了一种基于蛋白表达水平检测不同级别膀胱癌细胞裂解物的多抗体阵列生物芯片。将半乳糖凝集素-1 (Galectin-1)和乳酸脱氢酶B (Lactate Dehydrogenase B, LDH-B)两种抗体修饰的纳米探针通过连续的DEP操作步骤(聚焦、引导和捕获)以阵列方式固定，因此无需复杂的微通道设计，即可在单个芯片上实现两种不同的蛋白质水平。DEP芯片的结构由三个电极层组成，顶部电极由氧化铟锡(ITO)制成，中间电极位于SU-8表面，用于聚焦和引导纳米探针，底部ITO点状电极位于SU-8腔下，用于捕获纳米探针。在我们的实验中，将两种不同级别的膀胱癌细胞裂解物(I级:RT4和III级:T24)引入免疫传感器，Gal-1和LDH-B的表达水平对RT4和T24细胞裂解物表现出不同的荧光反应。因此，在T24细胞裂解液中，Gal-1的荧光强度高于LDH-B;而RT4的反应则相反，即ldl - b > Gal-1。因此，本发明的多抗体免疫传感器可实现不同级别膀胱癌细胞的检测。此外，纳米探针的DEP操作也证明了单芯片多生物标志物阵列用于即时诊断的可行性。
{"title":"Multi-antibody array chip for immunoassay of different-grade bladder cancer cell lysates based on DEP manipulations of nanoprobes","authors":"C. Chuang, Jing-Wei Ju, Kai-Chieh Chang, Yao-Wei Huang, C. Jen","doi":"10.1109/NEMS.2014.6908889","DOIUrl":"https://doi.org/10.1109/NEMS.2014.6908889","url":null,"abstract":"This paper develops a multi-antibody array biochip for detection of different-grade bladder cancer cell lysates based on protein expression level. Two nanoprobes modified with antibodies, Galectin-1 (Gal-1) and Lactate Dehydrogenase B (LDH-B), were immobilized in an array manner by sequent DEP manipulation steps including focusing, guiding and trapping, therefore, two different protein levels can be achieved in single chip without complex microchannel design. The structure of DEP chip consists of three electrode layers, top electrode made of Indium Tin Oxide (ITO), middle Au electrode on SU-8 surface for focusing and guiding of nanoprobes, and bottom ITO dot electrodes under SU-8 cavity for trapping of nanoprobes. In our experiments, two different grade bladder cancer cell lysates (grade I:RT4 and grade III:T24) were introduced into immunosensor, the expression levels of Gal-1 and LDH-B displayed different fluorescent responses for RT4 and T24 cell lysates. Accordingly, the fluorescence intensity of Gal-1 is higher than the value of LDH-B for T24 cell lysate; however, the response for RT4 is opposite, i.e. LDH-B > Gal-1. Consequently, determining of different-grade bladder cancer cell can be achieved by using present multi-antibody immunosensor. In addition, the DEP manipulations of nanoprobes also demonstrate the feasibility for multi-biomarker array in single chip for point-of-care diagnostics.","PeriodicalId":22566,"journal":{"name":"The 9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS)","volume":"39 1","pages":"626-627"},"PeriodicalIF":0.0,"publicationDate":"2014-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82243982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2014-04-13DOI: 10.1109/NEMS.2014.6908828
C. Lu, Kerwin Wang
This paper demonstrates a novel magnetic induced injection method of ferromagnetic composite to build electrically conductive through-silicon vias (TSVs). The through conductive via is filled with conductive ferromagnetic composite by attractive magnetic force. The composite is made of the mixture of silver and iron nanoparticles. SU-8 2002 is covered on the side walls of via as an insulating material by a low vacuum suction. After thermal curing process, the dielectric layer can possess an electric field as high as 5×106 V/cm. All of the fabrication steps are completed below 100 C. The TSVs can allow a current density of 6×107 A/m2. The leakage current is 2×10-6 A at 50 V. After DC electrical sintering, the resistances of each TSV is less than 0.85 Ω.
介绍了一种新型铁磁复合材料的磁致注入制备导电硅通孔的方法。通过吸引磁力填充导电铁磁性复合材料。这种复合材料是由银和铁纳米粒子的混合物制成的。SU-8 2002通过低真空吸盘作为绝缘材料覆盖在通孔侧壁上。经热固化处理后，介质层的电场可高达5×106 V/cm。所有的制造步骤都在100℃以下完成，tsv可以允许电流密度为6×107 a /m2。在50v时漏电流为2×10-6 A。直流电烧结后，各TSV的电阻均小于0.85 Ω。
{"title":"Filling through-silicon vias with conductive ferromagnetic silver-iron composite","authors":"C. Lu, Kerwin Wang","doi":"10.1109/NEMS.2014.6908828","DOIUrl":"https://doi.org/10.1109/NEMS.2014.6908828","url":null,"abstract":"This paper demonstrates a novel magnetic induced injection method of ferromagnetic composite to build electrically conductive through-silicon vias (TSVs). The through conductive via is filled with conductive ferromagnetic composite by attractive magnetic force. The composite is made of the mixture of silver and iron nanoparticles. SU-8 2002 is covered on the side walls of via as an insulating material by a low vacuum suction. After thermal curing process, the dielectric layer can possess an electric field as high as 5×106 V/cm. All of the fabrication steps are completed below 100 C. The TSVs can allow a current density of 6×107 A/m2. The leakage current is 2×10-6 A at 50 V. After DC electrical sintering, the resistances of each TSV is less than 0.85 Ω.","PeriodicalId":22566,"journal":{"name":"The 9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS)","volume":"23 1","pages":"366-369"},"PeriodicalIF":0.0,"publicationDate":"2014-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81492229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2014-04-13DOI: 10.1109/NEMS.2014.6908845
Yu-Jui Che, Huei-Wen Wu, Lein-Yu Hung, Hwan-You Chang, Kuan Wang, Gwo-Bin Lee
Reagents binding specifically to target molecules are essential tools for clinical diagnosis and targeted therapy. Screening of target cell-surface specific affinity reagents with bench-top methods has some drawbacks, including time-consuming, labor-intensive and requirement of large-scale instrument. Microfluidic platforms may overcome these drawbacks because they could automate and complete the screening process within a shorter period of time. Phage display is a promising technology in selection of cell-surface specific peptides. In recent years, a subpopulation of tumor cells named cancer stem cells is believed to be the tumorigenic cells and closely associated with metastasis. A specific peptide that can recognize and differentiate cancer stem cells from the rest of cancer cell population is therefore useful for early diagnosis and targeted therapy. In this study, selection of M13 phage displayed peptides that bind with colon cancer cells and colon cancer stem cells using an integrated microfluidic system was successfully demonstrated. Compared with the traditional methods, the total selection process was shortened to 36 hours while traditional method needs almost a month. More importantly, the screening process can be automated and performed on a single microfluidic chip. The developed technique may be promising for early diagnosis of cancer and target therapeutics.
{"title":"An integrated microfluidic system for screening of peptides specific to colon cancer cells and colon cancer stem cells using the phage display technology","authors":"Yu-Jui Che, Huei-Wen Wu, Lein-Yu Hung, Hwan-You Chang, Kuan Wang, Gwo-Bin Lee","doi":"10.1109/NEMS.2014.6908845","DOIUrl":"https://doi.org/10.1109/NEMS.2014.6908845","url":null,"abstract":"Reagents binding specifically to target molecules are essential tools for clinical diagnosis and targeted therapy. Screening of target cell-surface specific affinity reagents with bench-top methods has some drawbacks, including time-consuming, labor-intensive and requirement of large-scale instrument. Microfluidic platforms may overcome these drawbacks because they could automate and complete the screening process within a shorter period of time. Phage display is a promising technology in selection of cell-surface specific peptides. In recent years, a subpopulation of tumor cells named cancer stem cells is believed to be the tumorigenic cells and closely associated with metastasis. A specific peptide that can recognize and differentiate cancer stem cells from the rest of cancer cell population is therefore useful for early diagnosis and targeted therapy. In this study, selection of M13 phage displayed peptides that bind with colon cancer cells and colon cancer stem cells using an integrated microfluidic system was successfully demonstrated. Compared with the traditional methods, the total selection process was shortened to 36 hours while traditional method needs almost a month. More importantly, the screening process can be automated and performed on a single microfluidic chip. The developed technique may be promising for early diagnosis of cancer and target therapeutics.","PeriodicalId":22566,"journal":{"name":"The 9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS)","volume":"52 1","pages":"440-443"},"PeriodicalIF":0.0,"publicationDate":"2014-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87538907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2014-04-13DOI: 10.1109/NEMS.2014.6908771
Amarendra Kumar, Kunal Kashyap, K. Liao, M. T. Hou, J. Yeh
Glass nanostructures were fabricated by wet chemical etching instead of using the complicated nanolithography and expensive dry etching process. Super hydrophilic glass with contact angle of 1° was created using this nanostructure with 92% transparency. Self-assembled monolayer of Perfluorodecyl-trichlorosilane (FDTS) was used to further make the glass super hydrophobic with contact angle of 151° without affecting its transparency. Polysilicon layer of 300 nm was deposited on glass substrate first and nanostructures were fabricated in this layer by metal assisted wet chemical etching. Thermal oxidation converted the silicon nanostructure to silicon dioxide nanostructure. This technology is producible on mass scale and useful in preparation of anti-fogging and self-cleaning glass.
{"title":"Super hydrophobic/super hydrophilic transparent nanostructured glass fabricated by wet etching","authors":"Amarendra Kumar, Kunal Kashyap, K. Liao, M. T. Hou, J. Yeh","doi":"10.1109/NEMS.2014.6908771","DOIUrl":"https://doi.org/10.1109/NEMS.2014.6908771","url":null,"abstract":"Glass nanostructures were fabricated by wet chemical etching instead of using the complicated nanolithography and expensive dry etching process. Super hydrophilic glass with contact angle of 1° was created using this nanostructure with 92% transparency. Self-assembled monolayer of Perfluorodecyl-trichlorosilane (FDTS) was used to further make the glass super hydrophobic with contact angle of 151° without affecting its transparency. Polysilicon layer of 300 nm was deposited on glass substrate first and nanostructures were fabricated in this layer by metal assisted wet chemical etching. Thermal oxidation converted the silicon nanostructure to silicon dioxide nanostructure. This technology is producible on mass scale and useful in preparation of anti-fogging and self-cleaning glass.","PeriodicalId":22566,"journal":{"name":"The 9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS)","volume":"63 1","pages":"113-116"},"PeriodicalIF":0.0,"publicationDate":"2014-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78245692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2014-04-13DOI: 10.1109/NEMS.2014.6908820
Wei Ma, Ronghao Zeng, Dayu Liu, Y. Zohar, Yi-Kuen Lee
A SOI technology-based micro filtration system for circulating tumor cells (CTCs) isolation and enumeration is reported; this is in contrast of using single-crystal-silicon wafers resulting in poor etching uniformity. This microsystem consists of a silicon filter enclosed with PDMS layers, a digital syringe pump, a fluorescence microscope, a digital CCD camera in combination with the CellProfiler software for rapid cell enumeration. Human cervical cancer cells (HeLa cells) were used for preliminary tests of the system performance. 3D CFD simulations demonstrate that the velocity and shear stress can be well controlled to avoid hydrodynamic cell lysis. In comparing with our previous CTC chip using polycarbonate (PC) filter membrane, the SOI-based system has a much improved CTC capturing and enumeration performance.
{"title":"SOI technology-based microfiltration system for circulating tumor cells isolation and enumeration","authors":"Wei Ma, Ronghao Zeng, Dayu Liu, Y. Zohar, Yi-Kuen Lee","doi":"10.1109/NEMS.2014.6908820","DOIUrl":"https://doi.org/10.1109/NEMS.2014.6908820","url":null,"abstract":"A SOI technology-based micro filtration system for circulating tumor cells (CTCs) isolation and enumeration is reported; this is in contrast of using single-crystal-silicon wafers resulting in poor etching uniformity. This microsystem consists of a silicon filter enclosed with PDMS layers, a digital syringe pump, a fluorescence microscope, a digital CCD camera in combination with the CellProfiler software for rapid cell enumeration. Human cervical cancer cells (HeLa cells) were used for preliminary tests of the system performance. 3D CFD simulations demonstrate that the velocity and shear stress can be well controlled to avoid hydrodynamic cell lysis. In comparing with our previous CTC chip using polycarbonate (PC) filter membrane, the SOI-based system has a much improved CTC capturing and enumeration performance.","PeriodicalId":22566,"journal":{"name":"The 9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS)","volume":"12 1","pages":"333-336"},"PeriodicalIF":0.0,"publicationDate":"2014-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78360904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2014-04-13DOI: 10.1109/NEMS.2014.6908866
Yiting Yu, H. Zappe
Three-dimensional (3D) plasmon Talbot effect is experimentally investigated on three finite-sized, two-dimensional (2D) periodical arrays composed of subwavelength nanoholes with different fill factors. By using water as the output medium, both the focusing behavior and the plasmon Talbot revivals are clearly observed even when the operating wavelength is larger than the array period. And theoretically, with the output material having a refractive index of n, the operating wavelength to realize the plasmon Talbot effect can be enlarged by a factor of n-1. The integral and fractional plasmon Talbot revivals reproduce the device pattern with rich subwavelength hotspots (0.56~0.72λ) in exactly the same array period, which shows a great prospect for the low-cost, large-scale micro- and nanolithography. The preliminary experimental results indicate that the fill factor doesn't play an obvious influence on the size of the achieved plasmon Talbot hotspots.
{"title":"Experimental investigation of 3D plasmon talbot effect","authors":"Yiting Yu, H. Zappe","doi":"10.1109/NEMS.2014.6908866","DOIUrl":"https://doi.org/10.1109/NEMS.2014.6908866","url":null,"abstract":"Three-dimensional (3D) plasmon Talbot effect is experimentally investigated on three finite-sized, two-dimensional (2D) periodical arrays composed of subwavelength nanoholes with different fill factors. By using water as the output medium, both the focusing behavior and the plasmon Talbot revivals are clearly observed even when the operating wavelength is larger than the array period. And theoretically, with the output material having a refractive index of n, the operating wavelength to realize the plasmon Talbot effect can be enlarged by a factor of n-1. The integral and fractional plasmon Talbot revivals reproduce the device pattern with rich subwavelength hotspots (0.56~0.72λ) in exactly the same array period, which shows a great prospect for the low-cost, large-scale micro- and nanolithography. The preliminary experimental results indicate that the fill factor doesn't play an obvious influence on the size of the achieved plasmon Talbot hotspots.","PeriodicalId":22566,"journal":{"name":"The 9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS)","volume":"1 1","pages":"532-535"},"PeriodicalIF":0.0,"publicationDate":"2014-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78782437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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