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Effect of phenylalanine and tryptophane on chlorophyll and phenolic content, and the antioxidant activity of sweet basil (Ocimum basilicum L.) under drought conditions 苯丙氨酸和色氨酸对干旱条件下甜罗勒(Ocimum basilicum L.)叶绿素和酚含量以及抗氧化活性的影响
IF 0.9 4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-12-20 DOI: 10.13080/z-a.2023.110.038
N. Burbulis, Justina Deveikyte, A. Baltušnikienė, A. Blinstrubienė
Sweet basil ( Ocimum basilicum L.) is an endemic annual mucilaginous spicy herb plant of the mint family (Lamiaceae), growing wild in subtropical and tropical areas of America, Africa, Asia, and in southern Europe. Basil belongs to the aromatic plants that are grown all over the world nowadays. To increase the amount of phenolic and other biologically active compounds in plants, safe, effective, and ecological ways to achieve this are constantly being sought. The greatest economic losses worldwide are due to the negative effects of drought stress and extreme temperatures on plant morphological, physiological, and biochemical characteristics limiting crop growth and productivity. The aim of the study was to evaluate the effect of phenylalanine and tryptophane on the content of chlorophyll and phenolic, and the antioxidant activity of basil under drought conditions. Foliar spraying with phenylalanine and tryptophane to drought-stressed plants increased the chlorophyll a and b content. Application of phenylalanine significantly increased the total phenolic content (TPC) in the cultivar ‘Aromatico Della Riviera Ligure’, while foliar spraying with tryptophane increased TPC in the drought-stressed ‘Palla Compato’ and ‘Toscano’. Foliar spraying with phenylalanine and tryptophane to drought-stressed plants increased the antioxidant activity measured by the DPPH method. Principal component analysis showed that the first factor was highly and positively related to all parameters studied. Hierarchical clustering analysis showed that the first cluster was formed due to drought-stressed ‘Aromatico Della Riviera Ligure’, ‘Cinnamon’, and ‘Lemon’. The second cluster consisted of well-watered ‘Aromatico Della Riviera Ligure’, ‘Cinnamon’, and ‘Lemon’, and drought-stressed and phenylalanine-sprayed ‘Aromatico Della Riviera Ligure’ and ‘Palla Compato’. The third cluster consisted of the combinations of other cultivars and treatments tested.
甜罗勒(Ocimum basilicum L.)是薄荷科(Lamiaceae)特有的一年生粘液性辛辣草本植物,野生于美洲、非洲、亚洲和南欧的亚热带和热带地区。罗勒属于芳香植物,如今在世界各地都有种植。为了增加植物中酚类和其他生物活性化合物的含量,人们一直在寻找安全、有效和生态的方法。全世界最大的经济损失是由于干旱胁迫和极端温度对植物形态、生理和生化特性的负面影响,限制了作物的生长和产量。本研究旨在评估苯丙氨酸和色氨酸对干旱条件下罗勒叶绿素和酚类含量以及抗氧化活性的影响。向干旱胁迫植物叶面喷洒苯丙氨酸和色氨酸可提高叶绿素 a 和 b 的含量。喷施苯丙氨酸能显著提高栽培品种 "Aromatico Della Riviera Ligure "的总酚含量(TPC),而叶面喷施色氨酸能提高干旱胁迫下 "Palla Compato "和 "Toscano "的总酚含量。向干旱胁迫植物叶面喷洒苯丙氨酸和色氨酸可提高用 DPPH 法测量的抗氧化活性。主成分分析表明,第一个因子与研究的所有参数高度正相关。层次聚类分析显示,干旱胁迫的 "Aromatico Della Riviera Ligure"、"肉桂 "和 "柠檬 "形成了第一个聚类。第二组由水分充足的 "Aromatico Della Riviera Ligure"、"肉桂 "和 "柠檬",以及干旱胁迫和苯丙氨酸喷洒的 "Aromatico Della Riviera Ligure "和 "Palla Compato "组成。第三组包括其他栽培品种和试验处理的组合。
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引用次数: 0
Productivity of the warm-climate crop sweet potato (Ipomoea batatas L.) in northern latitudes 北纬地区暖温带作物甘薯(Ipomoea batatas L.)的生产力
IF 0.9 4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-12-20 DOI: 10.13080/z-a.2023.110.035
A. Kännaste, Ivar Zekker, T. Tosens, Liisa Kübarsepp, E. Runno-Paurson, Ü. Niinemets
Sweet potato ( Ipomoea batatas (L.) Lam.) has a potential to become a new field crop for higher latitudes. Sweet potato was cultivated for the first time in the fields of Estonia. During the study, the photosynthetic characteristics and yields of sweet potato cultivars ‘Evangeline’ and ‘Covington’ were investigated under fertilisation with two nitrogen (N) rates (0 and 100 kg ha −1 N) and various fertilisers in 2018, 2019 and 2021. Variation in fertiliser rate and composition had no effect on CO 2 assimilation rate ( A ) and stomatal conductance ( g s ). The negative relationship between photosynthetic nitrogen use efficiency (PNUE) and intrinsic water-use efficiency (iWUE) among cultivars and treatments indicated that water availability could limit nutrient availability and ultimately reduce the potential yield in northern growing regions. The highest sweet potato tuber yield (t ha −1 ) was obtained from ‘Evangeline’ and ‘Covington’ at N0 in 2021. A comparison of yields of 2018 and 2021 experiments suggests that sweet potatoes can be grown in high latitudes. It can be concluded that depending on weather conditions, cultivars and soil properties, fertilisers can promote high plant productivity. Further studies should focus on sweet potato plants traits that enable the efficient use of nutrients and water during a short and potentially dry growing season.
甘薯(Ipomoea batatas (L.) Lam.)有可能成为高纬度地区新的大田作物。爱沙尼亚首次在田间种植甘薯。研究期间,调查了 2018 年、2019 年和 2021 年在施用两种氮(N)率(0 和 100 千克/公顷-1 N)和各种肥料的情况下甘薯栽培品种 "Evangeline "和 "Covington "的光合特性和产量。施肥量和成分的变化对二氧化碳同化率(A)和气孔导度(g s)没有影响。不同栽培品种和处理的光合氮利用效率(PNUE)与内在水利用效率(iWUE)之间存在负相关关系,这表明在北方种植区,水分供应可能会限制养分供应,并最终降低潜在产量。2021 年,"Evangeline "和 "Covington "在氮为 0 的条件下获得了最高的甘薯块茎产量(吨公顷-1)。2018 年和 2021 年试验的产量比较表明,甘薯可以在高纬度地区种植。可以得出结论,根据天气条件、栽培品种和土壤特性,肥料可以促进植物高产。进一步的研究应侧重于甘薯植物的性状,以便在短暂且可能干旱的生长季节有效利用养分和水分。
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引用次数: 0
Rutin hydrate induces autophagic cell death and oxidative stress response in phytopathogenic fungus Fusarium graminearum 水合芦丁诱导植物病原真菌禾谷镰刀菌的自噬细胞死亡和氧化应激反应
IF 0.9 4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-12-20 DOI: 10.13080/z-a.2023.110.042
Emre Yörük
Fusarium graminearum infects cereal crops of high commercial and nutritional value such as barley, wheat, and maize, and causes various diseases. Due to the increasing world population and changing climatic conditions, the importance of controlling this pathogen is increasing. The aim of this study was to investigate the potential antifungal effect of the flavonoid compound rutin hydrate (RH) on the F. graminearum PH-1 reference strain at different levels. The EC 50 (half effective concentration) value was determined by the agar dilution method on the PDA (potato dextrose agar) medium containing different concentrations of rutin hydrate. To examine the effect of rutin hydrate on cell viability, the WST-1 toxicity test was performed. To determine the effect of rutin hydrate on genomic stability and methylation profiles, the CRED-RA (coupled restriction enzyme digestion-random amplification) analysis was performed. To determine the expression levels of atg5 , cat , kmet1 , sporulation ( mgv1 ), and apoptosis ( mst20 ) related genes, the qRT-PCR (quantitative real-time polymerase chain reaction) was performed. To detect the presence of oxidative stress and autophagy, the DCF-DA (2’,7’-dichlorofluorescein) and MDC (monodansylcadaverine) staining tests were used. The EC 50 value of rutin hydrate was determined as 580 µg mL −1 . The WST-1 analysis revealed that rutin hydrate caused cytotoxicity (58.53%) in F. graminearum ( p < 0.05). In the CRED-RA analysis, genomic template stability (GTS) decreased by 9.53%, and the methylation levels for Hap II and Msp I enzymes were 11.6% and 5.24%, respectively. There was a significant difference in the atg5 (2.66 ± 0.48; p < 0.01), cat (3.00 ± 0.46; p < 0.01), and kmet1 (6.29 ± 2.24; p < 0.05) expression in the rutin hydrate-treated samples. Autophagy and oxidative stress response with a significant difference ( p < 0.05) in the rutin hydrate-treated F. graminearum was confirmed by DCF-DA and MDC tests. This is the first report demonstrating the antifungal effect of rutin hydrate on F. graminearum at physiological, transcript, and epigenetic levels.
禾谷镰刀菌(Fusarium graminearum)会侵染大麦、小麦和玉米等具有较高商业和营养价值的谷类作物,并引发各种病害。由于世界人口的增加和气候条件的变化,控制这种病原体的重要性与日俱增。本研究旨在探讨黄酮类化合物水合芦丁(RH)在不同浓度下对禾谷镰孢属 PH-1 参考菌株的潜在抗真菌作用。在含有不同浓度水合芦丁的 PDA(马铃薯葡萄糖琼脂)培养基上,采用琼脂稀释法测定 EC 50(半数有效浓度)值。为检测芦丁水合物对细胞活力的影响,进行了 WST-1 毒性试验。为确定水合芦丁对基因组稳定性和甲基化图谱的影响,进行了 CRED-RA(耦合限制性酶消化-随机扩增)分析。为确定 atg5、cat、kmet1、孢子(mgv1)和凋亡(mst20)相关基因的表达水平,进行了 qRT-PCR(定量实时聚合酶链反应)分析。为了检测氧化应激和自噬的存在,使用了 DCF-DA(2',7'-二氯荧光素)和 MDC(单丹参素)染色试验。芦丁水合物的 EC 50 值被确定为 580 µg mL -1 。WST-1 分析表明,水合芦丁对禾谷镰刀菌具有细胞毒性(58.53%)(p < 0.05)。在 CRED-RA 分析中,基因组模板稳定性(GTS)降低了 9.53%,Hap II 和 Msp I 酶的甲基化水平分别为 11.6% 和 5.24%。在水合芦丁处理的样本中,atg5(2.66 ± 0.48;p < 0.01)、cat(3.00 ± 0.46;p < 0.01)和 kmet1(6.29 ± 2.24;p < 0.05)的表达量有明显差异。经 DCF-DA 和 MDC 测试证实,自噬和氧化应激反应在水合芦丁处理的禾谷镰刀菌中有显著差异(p < 0.05)。这是第一份在生理、转录本和表观遗传水平上证明水合芦丁对禾谷镰刀菌有抗真菌作用的报告。
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引用次数: 0
The effect of sublethal doses of Bacillus thuringiensis Berliner on Tuta absoluta (Meyrick) on resistant and susceptible tomato cultivars 亚致死剂量的柏林苏云金芽孢杆菌对抗性和易感番茄栽培品种上的 Tuta absoluta (Meyrick) 的影响
IF 0.9 4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-12-20 DOI: 10.13080/z-a.2023.110.041
Limo Khatami, Akbar Ghassemi-Kahrizeh, Abbas Hosseinzadeh, S. Aramideh
The effect of Bacillus thuringiensis ( Bt ) Berliner on the Tuta absoluta (Meyrick) on two resistant (‘King Stone’) and susceptible (‘Rio Grande’) tomato ( Solanum lycopersicum L.) cultivars was studied under laboratory conditions. In the bioassay tests, the lethal concentration (LC 50 ) values of Bt were determined for the 2nd-and 3rd-instar larvae of T. absoluta on two cultivars. In another experiment, the effect of sublethal doses of Bt on 2nd-and 3rd-instar larvae on two susceptible and resistant cultivars was determined. Also, the nutritional indices and the enzyme content of larvae on two susceptible and resistant cultivars under the sublethal doses of Bt were investigated. All nutritional indices and the enzyme content of the treated larvae were significantly different compared to those of the control group. The lowest efficiency of the conversion of ingested food (7.82%), the efficiency of the conversion of digested food (4.34%), the relative consumption rate (22.29 mg mg −1 day −1 ), and the relative growth rate (1.16 mg mg −1 day −1 ) was determined for the resistant cultivar ‘King Stone’ at the LC 25 concentration of Bt . The lowest amount of α-amylase (0.98 nM min −1 mg −1 protein), protease (12.61 µM min −1 mg −1 protein), and lipase (0.182 nM min −1 mg −1 protein) and the highest amount of phenoloxidase (0.337 µM min −1 mg −1 protein) were determined for the resistant cultivar ‘King Stone’ at the LC 25 concentration of Bt . The results showed that the integration of Bt application and host plant resistance significantly increased the efficiency of Bt .
在实验室条件下研究了苏云金芽孢杆菌(Bt)Berliner 对 Tuta absoluta(Meyrick)在两个抗性('King Stone')和感性('Rio Grande')番茄(Solanum lycopersicum L.)栽培品种上的影响。在生物测定试验中,测定了 Bt 对两个栽培品种上 T. absoluta 二、三龄幼虫的致死浓度(LC 50)值。在另一项实验中,测定了亚致死剂量的 Bt 对两种易感和抗性栽培品种上二三龄幼虫的影响。此外,还研究了亚致死剂量 Bt 对两种易感和抗性栽培品种上幼虫的营养指数和酶含量。与对照组相比,处理组幼虫的所有营养指标和酶含量都有显著差异。在 Bt 的 LC 25 浓度下,抗性栽培品种 "King Stone "的摄入食物转化效率(7.82%)、消化食物转化效率(4.34%)、相对消耗率(22.29 mg mg -1 day -1 )和相对生长率(1.16 mg mg -1 day -1 )最低。在 Bt 浓度为 LC 25 时,抗性品种'King Stone'的α-淀粉酶(0.98 nM min -1 mg -1 蛋白质)、蛋白酶(12.61 µM min -1 mg -1 蛋白质)和脂肪酶(0.182 nM min -1 mg -1 蛋白质)含量最低,酚氧化酶(0.337 µM min -1 mg -1 蛋白质)含量最高。结果表明,Bt 应用与寄主植物抗性的结合大大提高了 Bt 的效率。
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引用次数: 0
The environmental and genetic factors affect the productivity and quality of potato cultivars 环境和遗传因素影响马铃薯栽培品种的产量和质量
IF 0.9 4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-12-20 DOI: 10.13080/z-a.2023.110.036
L. Prysiazhniuk, T. Sonets, Yu.V. Shytikova, S. Melnyk, I. Dikhtiar, Kostiantyn Mazhuha, Borys Sorochinsky
This study aimed to evaluate the impact of environmental and genetic factors on the yield, starch, and dry matter content in tubers of potato ( Solanum tuberosum L.) cultivars belonging to different maturity groups considering cultivars diversity according to SSR markers. Twenty-four very early, early, medium, and late potato cultivars were evaluated. Field experiments were conducted in 2018–2020 in two locations: Forest Steppe and Forest zones of Ukraine. The SSRs analysis was carried out in 2021 using eight markers: STM0019, STM3009, STM2005, STM2028, STM3012, STM3023, STM5136
本研究旨在根据 SSR 标记评估环境和遗传因素对不同成熟度组马铃薯(Solanum tuberosum L.)栽培品种块茎产量、淀粉和干物质含量的影响。对 24 个极早熟、早熟、中熟和晚熟马铃薯栽培品种进行了评估。田间试验于 2018-2020 年在两个地点进行:乌克兰的森林草原区和森林区。2021 年使用 8 个标记进行了 SSR 分析:STM0019、STM3009、STM2005、STM2028、STM3012、STM3023、STM5136
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引用次数: 0
The effect of nitrogen use efficiency on significant traits of potato starch production 氮利用效率对马铃薯淀粉生产重要性状的影响
IF 0.9 4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-12-20 DOI: 10.13080/z-a.2023.110.037
I. Skrabule, Lasma Rabante-Hane, I. Dimante, Inese Taškova, Sergii Sviatchenko
The improvement of nitrogen use efficiency (NUE) in potato ( Solanum tuberosum L.) can reduce the required N input. As a result, economic benefits will increase, and environmental pollution will decrease due to N loss. The aim of the study was to determine the NUE of potato genotypes and to evaluate the relationship of NUE with the tuber yield, starch yield
提高马铃薯(Solanum tuberosum L.)的氮利用效率(NUE)可以减少所需的氮投入。因此,经济效益将增加,氮损失造成的环境污染将减少。本研究的目的是确定马铃薯基因型的氮利用效率,并评估氮利用效率与块茎产量、淀粉产量和茎叶产量的关系。
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引用次数: 0
Ameliorative effects of plant growth-promoting rhizobacteria on salinity tolerance of sweet basil (Ocimum basilicum L.) 植物生长促进根瘤菌对甜罗勒(Ocimum basilicum L.)耐盐性的改善作用
IF 0.9 4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-12-20 DOI: 10.13080/z-a.2023.110.039
Reza Salehi Molkabadi, Mina Alian, Gregory Bonito, K. Ghasemi, Maryam Mirtalebi, Fatemeh Raouf-Fard, Asghar Ramezanian
Salinity in soil and irrigation water has negative effects on plant morphology, physiology, and biochemical processes. In this study, an experiment was conducted to investigate the effect of two plant growth-promoting rhizobacteria (PGPR) Bacillus subtilis and Pseudomonas fluorescens on sweet basil ( Ocimum basilicum L.) growing under salinity stress: 0 (control, without NaCl), 50, and 100 mM NaCl
土壤和灌溉水中的盐分会对植物的形态、生理和生化过程产生负面影响。本研究通过实验研究了两种植物生长促进根瘤菌(PGPR)枯草芽孢杆菌(Bacillus subtilis)和荧光假单胞菌(Pseudomonas fluorescens)对盐胁迫下生长的甜罗勒(Ocimum basilicum L.)的影响:0(对照,不含 NaCl)、50 和 100 毫摩尔 NaCl
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引用次数: 0
Residual effects of repeated animal manure application on coarse-textured Ultisol, nutrient uptake and cucumber yield 重复施用动物粪便对粗质超土壤、养分吸收和黄瓜产量的残余影响
IF 0.9 4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-12-20 DOI: 10.13080/z-a.2023.110.040
B. O. Unagwu, Ikechukwu Onah, Grace Chibuike
One of the main UN Millennium Development Goals is eradication of poverty and hunger through food security. Unfortunately, this goal is difficult to achieve in Nigeria due to soil degradation, low soil fertility, and other agricultural challenges. The current research on food security in Nigeria is focused on improving soil fertility using low-cost organic amendments. Thus, in 2019, the study investigated the residual effects of the repeated animal manure application on the cucumber ( Cucumis sativus L.) yield, the nutrient uptake
联合国千年发展目标的主要目标之一是通过粮食安全消除贫困和饥饿。遗憾的是,由于土壤退化、土壤肥力低和其他农业挑战,这一目标在尼日利亚很难实现。目前,尼日利亚粮食安全研究的重点是利用低成本的有机添加剂提高土壤肥力。因此,在 2019 年,本研究调查了重复施用动物粪便对黄瓜(Cucumis sativus L.)产量、养分吸收和土壤肥力的影响。
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引用次数: 0
Quantifying maize phenology using beneficial microorganisms and residue management under deep tillage system 利用有益微生物和深耕系统下的残留物管理量化玉米物候期
IF 0.9 4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-12-20 DOI: 10.13080/z-a.2023.110.034
Haris Khan, Ahmad Khan, Sajid Khan, A. Anjum, Habib Akbar, Dost Muhammad
Residues differing in quality and incorporation depths are presumed to contribute differently to the improvement of soil properties through decomposition. Understanding the response of residue decomposition at different depths using tillage implements and effective microorganisms can help to develop strategies for improving soil properties. Therefore, three nitrogen (N) supplemented crop residues: wheat (Rw), chickpea (Rc), Rw + Rc, and no residue (R0) as a control, under three different tillage implements: cultivator (CT), mouldboard plough (MBP)
据推测,不同质量和掺入深度的残留物会通过分解对土壤性质的改善产生不同的作用。了解使用耕作机具和有效微生物在不同深度分解残留物的反应有助于制定改善土壤性质的策略。因此,在三种不同的耕作机具(耕作机 (CT)、模板犁 (MBP) 和无残留 (R0) 作对照的情况下,使用三种氮(N)补充作物残留:小麦 (Rw)、鹰嘴豆 (Rc)、Rw + Rc 和无残留 (R0)
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引用次数: 0
Changes in soil microbial community structure after fertilisation with organic fertilisers and biological additives 施用有机肥和生物添加剂后土壤微生物群落结构的变化
IF 0.9 4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-12-15 DOI: 10.13080/z-a.2023.110.033
Diana Sivojienė, A. Kačergius
Various forms of organic fertilisers are often used in agriculture. It is useful to have information on how microbial communities in arable land are affected by fertilisation with different types of fertilisers. One of the indicators of the stability and fertility of the soil ecosystem is the population structure and abundance of soil microorganisms. The aim of the study was to determine how fertilisation and biological additives affected the most active soil microorganisms of the studied functional groups in 2021 and 2022. It was found that organotrophic (113 ± 3 × 10 3 CFU g −1 ) and diazotrophic (161 ± 5 × 10 3 CFU g −1 ) bacteria were most abundant in the spring 2021. However, in 2022, the amount of organotrophs decreased, and their abundance was approximately the same throughout the growing season, probably due to a decrease in the amount of free organic matter in the soil. In 2022, the most abundant organotrophs (91 ± 2 × 10 3 CFU g −1 ) and diazotrophs (100 ± 1 × 10 3 CFU g −1 ) were also in the spring. Organic fertilisers and their different rates had a significant or very significant effect on the abundance of organotrophic, mineral nitrogen-assimilating, and non-symbiotic diazotrophic bacteria, except for diazotrophs in treatments with the biological additive Trichoderma spp. In 2021, nitrifiers (162 ± 5 × 10 3 CFU g −1 ) and fungi (2.29 ± 0.13 × 10 3 CFU g −1 ) were most abundant in the autumn. In 2022, nitrifiers were most abundant in the spring (128 ± 2 × 10 3 CFU g −1 ) but less abundant than in the autumn 2021. During the experimental period, the abundance of fungi and yeasts was highest in the autumn, and this trend was not affected by fertilisation. In the treatments with the biological additive Trichoderma spp., organic fertilisers significantly or very significantly affected the abundance of fungi and yeasts. However, no significant differences were found in the treatments with the biological additive Azotobacter spp.
农业中经常使用各种形式的有机肥料。了解耕地中的微生物群落如何受到不同类型肥料的影响是非常有用的。土壤微生物的种群结构和数量是衡量土壤生态系统稳定性和肥力的指标之一。这项研究的目的是确定施肥和生物添加剂如何影响 2021 年和 2022 年所研究功能群中最活跃的土壤微生物。研究发现,2021 年春季,有机营养细菌(113 ± 3 × 10 3 CFU g -1 )和重氮营养细菌(161 ± 5 × 10 3 CFU g -1 )的数量最多。然而,到了 2022 年,有机营养菌的数量有所减少,在整个生长季中,有机营养菌的数量大致相同,这可能是由于土壤中游离有机物的数量减少所致。2022 年,春季的有机营养体(91 ± 2 × 10 3 CFU g -1 )和重氮营养体(100 ± 1 × 10 3 CFU g -1 )含量最高。有机肥及其不同施肥量对有机营养细菌、矿物氮同化细菌和非共生重氮细菌的丰度有显著或非常显著的影响,但添加了毛霉属生物添加剂的处理中的重氮细菌除外。 在 2021 年,硝化细菌(162 ± 5 × 10 3 CFU g -1 )和真菌(2.29 ± 0.13 × 10 3 CFU g -1 )在秋季最多。2022 年,硝化细菌在春季最多(128 ± 2 × 10 3 CFU g -1 ),但比 2021 年秋季少。在实验期间,真菌和酵母菌的数量在秋季最多,这一趋势不受施肥的影响。在施用生物添加剂毛霉属的处理中,有机肥对真菌和酵母菌的数量有显著或非常显著的影响。然而,在施用生物添加剂 "Azotobacter spp.
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引用次数: 0
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