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Features of photosynthetic activity and water consumption of safflower 红花光合活性及耗水量特征
IF 0.9 4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2022-06-20 DOI: 10.13080/z-a.2022.109.016
O. Aliieva, A. Polyakov, E. Aliiev
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引用次数: 3
Effect of restricted grazing or feeding with total mix ration environments on the properties of milk quantity and quality from dairy cows of different genotypes 限制放牧和全混合日粮环境对不同基因型奶牛产奶量和品质的影响
IF 0.9 4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2022-06-20 DOI: 10.13080/z-a.2022.109.024
Kristina de Vitte, S. Kerzienė, J. Klementavičiūtė, Marius de Vitte, Vaida Dilbienė, R. Ugenskienė, E. Bartkienė, R. Stankevičius
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引用次数: 0
Biocontrol of Botrytis cinerea and promotion of tomato growth by local soil-borne Bacillus isolates 土传芽孢杆菌对番茄灰霉病的生物防治及对番茄生长的促进作用
IF 0.9 4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2022-06-20 DOI: 10.13080/z-a.2022.109.020
Vasiljka Dragić, D. Miljaković, J. Marinković, M. Ignjatov, D. Milošević, J. Vasin, A. Bulajić, M. Vojvodić, M. Ivanović
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引用次数: 0
Agro-biological traits of soybean cultivars, their yield quantity and quality under Central European conditions 中欧条件下大豆品种的农业生物学特性及其产量和品质
IF 0.9 4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2022-06-20 DOI: 10.13080/z-a.2022.109.014
József Csajbók, Erika Kutasy, A. A. Melash, I. Virág, É. Ábrahám
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引用次数: 2
Effect of endophytic bacteria isolates on growth and oxidative stress injury of transgenic tobacco shoots in vitro 内生细菌分离物对转基因烟草苗生长和氧化应激损伤的影响
IF 0.9 4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2022-06-20 DOI: 10.13080/z-a.2022.109.021
E. Andriūnaitė, Inga Tamošiūnė, D. Gelvonauskienė, J. Vinskienė, V. Stanys, D. Baniulis, R. Rugienius
Transgenic plants have a great potential for production of high-value molecules, including peptides, for medical or industrial applications. Cultivation of plant tissues in a contained environment, such as plant tissue culture in vitro , could effectively address safety issues associated with transgenic plants as well as strict manufacturing standards. However, unfavourable in vitro conditions can often lead to elevated stress levels and suppressed growth of in vitro tissues urging the need to improve plant adaptation to in vitro conditions. As the beneficial effect of endophytic bacteria on the growth of in vitro cultures has been described previously, the aim of the present study was to assess the effect of growth-promoting endophytic bacteria isolates of the Bacillus cereus group on the growth of transgenic tobacco shoots in vitro and the accumulation of recombinant protein (RP) construct comprising a green fluorescent protein and a collagen mimicking peptide conjugate. The research revealed that transgenic tobacco shoots had reduced growth and increased oxidative injury levels compared with the non-transgenic parental line. The inoculated bacteria isolates colonised plant tissues and were sustained at densities of 0.6–9.0 × 10 5 CFU g -1 of the shoot fresh weight for at least several passages. Enhanced, 18% to 30% higher, biomass accumulation compared to the uninoculated transgenic tobacco shoots, and 14% to 25% lower levels of the oxidative lipid injury marker malondialdehyde (MDA) were detected for three-week-old shoots co-cultivated with isolates of Bacillus mycoides Nt.10.1, B. toyonensis Nt.18 and B. wiedmannii Nt.3.2. B. wiedmannii isolate Nt.14.2 reduced oxidative stress injury symptoms but had no effect on shoot growth. Co-cultivation with endophytic isolates had no effect on the expression of the recombinant protein. These results suggest that co-cultivation with growth-promoting endophytic bacteria provides a viable solution to improve the growth of transgenic plant tissues used for recombinant protein production in vitro .
转基因植物具有生产高价值分子的巨大潜力,包括多肽,用于医疗或工业应用。在封闭环境中培养植物组织,如植物离体组织培养,可以有效解决转基因植物的安全性问题以及严格的制造标准。然而,不利的体外条件往往会导致体外组织的应激水平升高和生长抑制,迫切需要提高植物对体外条件的适应。由于内生细菌对体外培养物生长的有益作用已经被描述,本研究的目的是评估促生长内生细菌蜡样芽孢杆菌群分离物对转基因烟草芽的体外生长的影响,以及由绿色荧光蛋白和胶原模拟肽偶联物组成的重组蛋白(RP)构建物的积累。研究表明,与非转基因烟草亲本相比,转基因烟草幼芽的生长受到抑制,氧化损伤水平增加。接种后的细菌在植株组织中定植,并以茎部鲜重的0.6-9.0 × 10 5 CFU g -1的密度维持至少几代。与未接种转基因烟草芽孢杆菌Nt.10.1、toyonensis Nt.18和B. wiedmannii Nt.3.2的分离株共培养的3周龄芽孢的生物量积累比未接种转基因烟草芽增加了18% ~ 30%,氧化脂质损伤标志物丙二醛(MDA)水平降低了14% ~ 25%。魏德曼双歧杆菌分离株Nt.14.2可减轻氧化应激损伤症状,但对芽部生长无影响。与内生菌株共培养对重组蛋白的表达无影响。这些结果表明,与促生长内生细菌共同培养为提高转基因植物组织的生长提供了一种可行的解决方案,用于体外生产重组蛋白。
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引用次数: 1
Yield capacity and energy value of sorghum grain depending on the application of mineral fertilisers 施用矿肥对高粱籽粒产量和能量值的影响
IF 0.9 4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2022-06-20 DOI: 10.13080/z-a.2022.109.015
L. Pravdyva, V. Doronin, Viktoriia Dryha, V. Khakhula, Serhii P. Vakhniy, Іryna І. Mykolaiko
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引用次数: 2
Fluorescent in situ hybridisation in plants: new ways to link DNA sequence resources and chromosome loci – a review 植物荧光原位杂交:链接DNA序列资源和染色体位点的新方法综述
IF 0.9 4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2022-06-20 DOI: 10.13080/z-a.2022.109.022
V. Stakelienė, I. Pašakinskienė
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引用次数: 0
Short-term effect of heavy precipitation on nutrient leaching from arable sandy loam soil amended with fertiliser and biochar 强降水对施肥和生物炭改良沙壤土养分淋失的短期影响
IF 0.9 4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2022-03-30 DOI: 10.13080/z-a.2022.109.002
Tatiana V. Abramova, Natalya P. Buchkina
The purpose of this study was to assess the effect of biochar application on the leaching of the main nutrients from the topsoil of the arable sandy loam soil (Albic Luvisol) of North-Western Russia after application of mineral fertiliser in the conditions of heavy precipitation. A short-term laboratory experiment included 6 treatments: (1) without fertiliser and biochar (control), (2–3) soil + biochar (10 and 20 t ha-1), (4) soil + fertiliser, and (5–6) soil + biochar (10 and 20 t ha-1) + fertiliser. During the experiment, the soil in the cylinders was watered three times with high rates (345 cm3) of distilled water, corresponding to the maximum amount of daily precipitation (44 mm) observed in the natural conditions of the area. The leachate was collected (on the 1st, 2nd and 5th day of the experiment) and tested for pHH2O, available nitrogen (N-NO3 −, N-NH4 +), phosphorus (P2O5) and potassium (K2O). At the end of the experiment, separate soil samples were formed of the upper 8-cm and lower 4-cm soil layers in the cylinders. The pHH2O values, concentrations of N-NO3 −, N-NH4 +, P2O5 and K2O were determined in the samples. The results showed that the effect of biochar on the movement and accumulation of the main available nutrients in the soil after fertiliser application and under the effect of heavy precipitation was more pronounced at the higher biochar application rate of 20 t ha-1. Biochar application reduced the acidity of the leachate and the soil not only in the layer, where the biochar had been applied, but also in the lower layer affected by the leachate. Available NNH4 +, P2O5, and K2O were retained in the soil through biochar application for a short period of time, while N-NO3 − leaching was not affected. Overall, biochar can be used as a soil ameliorant, as even a short delay in the nutrient leaching is potentially beneficial to nutrient utilization of crops.
本研究的目的是评估在强降水条件下施用生物炭对俄罗斯西北部可耕地砂壤土(Albic Luvisol)施用矿物肥后表层土壤主要养分淋失的影响。短期室内试验包括6个处理:(1)无肥料和生物炭(对照),(2-3)土壤+生物炭(10和20 t ha-1),(4)土壤+肥料,(5-6)土壤+生物炭(10和20 t ha-1) +肥料。在实验过程中,用高倍率(345 cm3)蒸馏水浇灌瓶中的土壤三次,对应于该地区自然条件下观察到的最大日降水量(44 mm)。收集渗滤液(试验第1、2、5天),测定pHH2O、速效氮(N-NO3−、N-NH4 +)、磷(P2O5)、钾(K2O)。实验结束时,将圆柱体中上部8cm土层和下部4cm土层分别形成单独的土样。测定了样品中的pHH2O值、N-NO3−、N-NH4 +、P2O5和K2O的浓度。结果表明:施用20 t ha-1时,生物炭对施肥后和强降水条件下土壤中主要速效养分的运移和积累的影响更为显著;施用生物炭不仅降低了施用生物炭层的渗滤液酸度和土壤酸度,而且降低了受渗滤液影响的下层土壤酸度。施用生物炭能在短时间内保持土壤中有效的NNH4 +、P2O5和K2O,而N-NO3−淋溶不受影响。总的来说,生物炭可以用作土壤改良剂,因为即使养分淋失的短暂延迟也可能有利于作物的养分利用。
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引用次数: 0
Common bean seedlings show increased tolerance to cool temperatures when treated with progesterone, β-oestradiol, abscisic acid, and salicylic acid 用黄体酮、β-雌二醇、脱落酸和水杨酸处理后,普通豆苗对低温的耐受性增强
IF 0.9 4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2022-03-30 DOI: 10.13080/z-a.2022.109.006
Bagher Sheikhi Didani, Rahmi Dumlupinar
Cold stress has a significant effect on plant physiology inducing growth hindrance and reduced yields. This study was aimed to evaluate the effect of mammalian sex hormones (progesterone and β-estradiol), abscisic acid (ABA), and salicylic acid (SA) on plant growth, chlorophyll, malondialdehyde (MDA), hydrogen peroxide (H2O2) and the activities of anti-oxidative and nitrate assimilation enzymes in common bean (Phaseolus vulgaris L.) seedlings under cold stress. For this purpose, cold stress was conducted to 11-day-old seedlings in a climate chamber at 9/5°C (day/night), but for control, the temperature was set at 25/20°C (day/night). Progesterone (Pro) 10-9 M, βoestradiol (ES) 10-6 M, ABA 10-4 M, and SA 10-4 M were applied to 11-day-old seedlings using a handheld sprayer. The results of this experiment showed that cold stress significantly decreased plant growth, and it was followed by an increase in MDA and H2O2 content in common bean seedlings. However, the exogenous application of Pro, ES, ABA, and SA mitigated the adverse effect of cold stress in common bean seedlings. In this respect, cold stress decreased the activities of nitrate reductase (NR), nitrite reductase (NiR), and glutamine synthetase (GS) by 41.03%, 35.20%, and 47.54%, respectively, compared to control. However, under cold stress, Pro, ES, ABA, and SA treatments significantly increased NR, NiR, and GS activities by 41.87%, 14.80%, 33.87%, and 64.82 % for NR, 3.4%, 30.10%, 12.75%, and 33.12% for NiR, and 83.00%, 50.10%, 83.14%, and 9.34% for GS, respectively, compared to the plants treated with cold stress. These results suggest that exogenous application of Pro 10-9 M, ES 10-6 M, ABA 10-4 M, and SA 10-4 M after three days mitigate the unfavourable impact of cold stress in common bean seedlings by enhancing nitrate metabolism and antioxidant enzyme activities; their spraying on the leaves is beneficial for plant recovery and growth under cold stress.
冷胁迫对植物生理有显著影响,引起生长障碍和减产。本研究旨在研究哺乳动物性激素(孕酮和β-雌二醇)、脱落酸(ABA)和水杨酸(SA)对低温胁迫下普通豆幼苗生长、叶绿素、丙二醛(MDA)、过氧化氢(H2O2)以及抗氧化酶和硝酸盐同化酶活性的影响。为此,在9/5°C(昼/夜)的气候室中对11日龄幼苗进行冷胁迫,但作为对照,温度设置为25/20°C(昼/夜)。用手持式喷雾器将黄体酮(Pro) 10-9 M、β雌二醇(ES) 10-6 M、ABA 10-4 M和SA 10-4 M施用于11日龄的幼苗上。本试验结果表明,冷胁迫显著降低了普通豆苗的生长,随后导致普通豆苗MDA和H2O2含量升高。然而,外源施用Pro、ES、ABA和SA减轻了冷胁迫对普通豆幼苗的不利影响。因此,与对照相比,冷胁迫使玉米硝酸盐还原酶(NR)、亚硝酸盐还原酶(NiR)和谷氨酰胺合成酶(GS)活性分别降低了41.03%、35.20%和47.54%。而在冷胁迫下,Pro、ES、ABA和SA处理的NR、NiR和GS活性较冷胁迫分别提高了41.87%、14.80%、33.87%、64.82%、3.4%、30.10%、12.75%、33.12%和83.00%、50.10%、83.14%、9.34%。综上所述,3 d后外源施用Pro 10-9 M、ES 10-6 M、ABA 10-4 M和SA 10-4 M可通过提高硝酸盐代谢和抗氧化酶活性缓解冷胁迫对普通豆幼苗的不利影响;它们在叶片上喷洒有利于植物在寒冷胁迫下的恢复和生长。
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引用次数: 0
Assessment of an abandoned grassland community and the soil seed bank of a hilly relief 丘陵地带废弃草地群落与土壤种子库评价
IF 0.9 4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2022-03-30 DOI: 10.13080/z-a.2022.109.001
R. Skuodienė, V. Matyžiūtė
The importance of grasslands is closely linked to biodiversity and soil health. Changes in plant diversity reflect the environmental conditions and practices of grasslands management. The aim of this study was to investigate the impact of abandonment on the grassland community and soil seed bank under the conditions of a hilly relief. The experiment was carried out at the Vėžaičiai Branch of the Lithuanian Research Centre for Agriculture and Forestry on the hilly topography of Žemaičiai Highland. The study analyses long-term monitoring data of a soil erosion experiment set up on the slopes of 9–11° steepness. The soil of the southern exposition slope was a slightly eroded Eutric Retisol. In 1993, to protect the hill from erosion, a mixture of five grasses consisting of 20% timothy grass (Phleum pratense L.), 20% red fescue (Festuca rubra L.), 20% meadow grass (Poa pratensis L.), 20% white clover (Trifolium repens L.), and 20% common bird’s-foot trefoil (Lotus corniculatus L.) was sown in different parts of the hill: the summit, the midslope, and the footslope. The grassland had not been fertilised and used. After 27 years, 56 vascular plant species were identified. In the summit, midslope, and footslope parts of the hill, the relative abundance (P%) of the sown species was 17.2%, 23.9%, and 27.2%, respectively. A good growth of Festuca rubra did not depend on abandonment. In all parts of the hill, P% of other sown species significantly decreased. The lowvalue plants (Elytrigia repens L., Cirsium arvense L., and Equisetum arvense L.) spread in grasslands. The soil seed bank of abandoned grasslands was composed of the arable weeds: Chenopodium album L. was the dominant species in the soil seed bank of the summit of the hill (0–15 cm depth), while Stellaria media (L.) Vill in the soil seed bank of the midslope, 31.3% and 18.4%, respectively, of the total seed number.
草原的重要性与生物多样性和土壤健康密切相关。植物多样性的变化反映了环境条件和草原管理实践。本研究旨在探讨丘陵地形下撂荒对草地群落和土壤种子库的影响。这项试验是在立陶宛农业和林业研究中心Vėžaičiai分部在Žemaičiai高地的丘陵地形上进行的。本研究分析了在坡度为9 ~ 11°的斜坡上进行的土壤侵蚀试验的长期监测数据。南展览坡的土壤是一种轻微侵蚀的土壤。1993年,为了保护山免受侵蚀,在山顶、中坡和脚坡的不同地方播种了五种草的混合种,其中包括20%的蒂木草(Phleum pratense L.)、20%的红羊茅(Festuca rubra L.)、20%的草皮草(Poa pratensis L.)、20%的白三叶草(Trifolium repens L.)和20%的普通鸟脚三叶草(Lotus corniculatus L.)。草地没有施肥和利用。经过27年的研究,共鉴定出56种维管植物。在山顶、中坡和坡脚部分,播种物种的相对丰度(P%)分别为17.2%、23.9%和27.2%。红羊茅的良好生长并不依赖于遗弃。在丘陵各部位,其他播种树种的P%显著下降。低价值植物(Elytrigia repens L., cisium arvense L., Equisetum arvense L.)广泛分布于草原。撂荒草原土壤种子库以可耕地杂草为主,山顶土壤种子库(0 ~ 15 cm深度)以藜草(Chenopodium album L.)为优势种,而黄芩(Stellaria media, L.)为优势种。中坡土壤种子库种子数占总种子数的31.3%和18.4%。
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引用次数: 2
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Zemdirbyste-agriculture
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