Bioelectrochemistry最新文献_第8页

Microbiologically influenced corrosion of oil-water pipeline steel from local field failure case to specific Shewanella & Desulfovibrio corrosion highlights the significance of hydrocarbon-degrading bacteria 微生物对油水管道钢腐蚀的影响，从现场局部失效案例到具体的希瓦氏菌和脱硫弧菌腐蚀，凸显了烃类降解菌的重要性。

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2025-10-02 DOI: 10.1016/j.bioelechem.2025.109129

Yanran Wang , Shaomu Wen , Qiancheng Yu , Hongfa Huang , Yongfan Tang , Chao Miao , Yunqing Xia , Fang Guan

Unconventional natural gas pipeline corrosion is associated with microbes, especially sulfate-reducing bacteria (SRB), though it is uncertain if SRB's role is overemphasized. Using metagenomics, corrosion immersion, and microbial cultivation, diverse hydrocarbon-degrading microorganisms, such as Shewanella, in corroded pipeline rust layers, oil-water mixtures, and produced water from unconventional natural gas fields are identified. These bacteria use crude oil as a carbon source, accelerating pitting corrosion of carbon steel and forming corrosion product films (Pit_max = 28.96 μm). The 16S rRNA sequencing results show that Shewanella, prevalent in various steel service environments, is a potential key microorganism in pipeline corrosion. X70 steel exhibits lower electron transfer resistance than Desulfovibrio in the Shewanella medium. Shewanella's aerobic respiration degrades crude oil and oxidizes iron, speeding up iron oxide formation and magnesium phosphate precipitation. Microbial acidification of the oil-water medium also contributes to severe pitting corrosion beneath the oil film. Crude oil accelerates microbial growth. Thus, studying carbon steel corrosion in oil-water environments must consider the impact of hydrocarbon-degrading microorganisms.

非常规天然气管道的腐蚀与微生物有关，尤其是硫酸盐还原菌（SRB），尽管不确定SRB的作用是否被过分强调了。通过宏基因组学、腐蚀浸泡和微生物培养，可以在腐蚀的管道锈层、油水混合物和非常规气田的采出水中识别出多种碳氢化合物降解微生物，如希瓦氏菌。这些细菌以原油为碳源，加速碳钢的点蚀，形成腐蚀产物膜（Pitmax = 28.96 μm）。16S rRNA测序结果表明，希瓦氏菌普遍存在于各种钢铁使用环境中，是潜在的管道腐蚀关键微生物。X70钢在希瓦氏菌培养基中表现出比Desulfovibrio更低的电子转移电阻。希瓦氏菌的有氧呼吸降解原油并氧化铁，加速氧化铁的形成和磷酸镁的沉淀。油水介质的微生物酸化也会导致油膜下的严重点蚀。原油加速了微生物的生长。因此，研究碳钢在油水环境中的腐蚀必须考虑烃类降解微生物的影响。

{"title":"Microbiologically influenced corrosion of oil-water pipeline steel from local field failure case to specific Shewanella & Desulfovibrio corrosion highlights the significance of hydrocarbon-degrading bacteria","authors":"Yanran Wang , Shaomu Wen , Qiancheng Yu , Hongfa Huang , Yongfan Tang , Chao Miao , Yunqing Xia , Fang Guan","doi":"10.1016/j.bioelechem.2025.109129","DOIUrl":"10.1016/j.bioelechem.2025.109129","url":null,"abstract":"<div><div>Unconventional natural gas pipeline corrosion is associated with microbes, especially sulfate-reducing bacteria (SRB), though it is uncertain if SRB's role is overemphasized. Using metagenomics, corrosion immersion, and microbial cultivation, diverse hydrocarbon-degrading microorganisms, such as <em>Shewanella</em>, in corroded pipeline rust layers, oil-water mixtures, and produced water from unconventional natural gas fields are identified. These bacteria use crude oil as a carbon source, accelerating pitting corrosion of carbon steel and forming corrosion product films (Pit<sub>max</sub> = 28.96 μm). The 16S rRNA sequencing results show that <em>Shewanella</em>, prevalent in various steel service environments, is a potential key microorganism in pipeline corrosion. X70 steel exhibits lower electron transfer resistance than <em>Desulfovibrio</em> in the <em>Shewanella</em> medium. <em>Shewanella</em>'s aerobic respiration degrades crude oil and oxidizes iron, speeding up iron oxide formation and magnesium phosphate precipitation. Microbial acidification of the oil-water medium also contributes to severe pitting corrosion beneath the oil film. Crude oil accelerates microbial growth. Thus, studying carbon steel corrosion in oil-water environments must consider the impact of hydrocarbon-degrading microorganisms.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"168 ","pages":"Article 109129"},"PeriodicalIF":4.5,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145297890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioelectrochemical characterization of a biogas-producing microbial electrolysis cell (MEC): advantages and limitations of electroanalytical techniques 产气微生物电解池（MEC）的生物电化学表征：电分析技术的优点和局限性

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2025-09-30 DOI: 10.1016/j.bioelechem.2025.109128

Ariadna Segundo-Aguilar , Gabriel Trejo , Eligio P. Rivero , Fernando F. Rivera , Bibiana Cercado

Electroanalytical techniques can be used to provide new insights into the electrochemical response of microbial bioelectrodes with some limitations due to complex interphase biofilm-electrode. In this work, characterization techniques, including open-circuit voltage, cyclic voltammetry, and electrochemical impedance spectroscopy, were applied to the bioanode, while chronoamperometry was used for the MEC with nine combinations of applied voltage and biomass content for anodic biogas production.

The open-circuit potential was characteristic of the bioanode at −0.4 V ± 0.02 V vs. Ag/AgCl; the cumulative charge evolution allowed differentiation of operation conditions, reaching a total charge between 30C and 170C. Three distinct profiles were found among the voltammograms including oxidation peaks, oxidation waves and no faradaic signals. The charge transfer resistance estimated via electrochemical impedance spectroscopy only allowed differentiation of the final from the initial conditions. A nonlinear correlation was found between electrochemical parameters and biogas volume and its composition. This work presents a method for the systematic electroanalysis of bioelectrodes and provides information on their behavior under different biomass and applied voltage conditions.

电分析技术可以为研究微生物电极的电化学响应提供新的见解，但由于生物膜电极间相复杂，电分析技术存在一定的局限性。在这项工作中，包括开路电压、循环伏安法和电化学阻抗谱在内的表征技术被应用于生物阳极，而计时电流法则用于MEC，具有9种施加电压和生物质含量的组合，用于阳极沼气生产。在−0.4 V±0.02 V vs. Ag/AgCl时，生物阳极具有开路电位特征；累积电荷演变允许区分操作条件，达到30℃至170℃之间的总电荷。在伏安图中发现了氧化峰、氧化波和无法拉第信号三种不同的分布。通过电化学阻抗谱估计的电荷转移电阻只允许最终条件与初始条件的区别。电化学参数与沼气体积及其组成呈非线性相关。这项工作提出了一种系统电分析生物电极的方法，并提供了它们在不同生物量和施加电压条件下的行为信息。

{"title":"Bioelectrochemical characterization of a biogas-producing microbial electrolysis cell (MEC): advantages and limitations of electroanalytical techniques","authors":"Ariadna Segundo-Aguilar , Gabriel Trejo , Eligio P. Rivero , Fernando F. Rivera , Bibiana Cercado","doi":"10.1016/j.bioelechem.2025.109128","DOIUrl":"10.1016/j.bioelechem.2025.109128","url":null,"abstract":"<div><div>Electroanalytical techniques can be used to provide new insights into the electrochemical response of microbial bioelectrodes with some limitations due to complex interphase biofilm-electrode. In this work, characterization techniques, including open-circuit voltage, cyclic voltammetry, and electrochemical impedance spectroscopy, were applied to the bioanode, while chronoamperometry was used for the MEC with nine combinations of applied voltage and biomass content for anodic biogas production.</div><div>The open-circuit potential was characteristic of the bioanode at −0.4 V ± 0.02 V vs. Ag/AgCl; the cumulative charge evolution allowed differentiation of operation conditions, reaching a total charge between 30C and 170C. Three distinct profiles were found among the voltammograms including oxidation peaks, oxidation waves and no faradaic signals. The charge transfer resistance estimated via electrochemical impedance spectroscopy only allowed differentiation of the final from the initial conditions. A nonlinear correlation was found between electrochemical parameters and biogas volume and its composition. This work presents a method for the systematic electroanalysis of bioelectrodes and provides information on their behavior under different biomass and applied voltage conditions.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"168 ","pages":"Article 109128"},"PeriodicalIF":4.5,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145262282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Green electrochemical biosensor for food safety monitoring of carcinogenic acrylamide and formaldehyde 用于食品安全致癌物质丙烯酰胺和甲醛监测的绿色电化学生物传感器

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2025-09-30 DOI: 10.1016/j.bioelechem.2025.109122

Catarina Meliana , Michelle Tien Tien Tan , Hwei-San Loh , Weihua Meng , Cheng Heng Pang , Sze Shin Low

Food safety monitoring demands practical strategies for detecting chemical hazards such as acrylamide (AA) and formaldehyde (FA), both classified as probable human carcinogens with dietary exposures often exceeding tolerable limits. Conventional methods including GC–MS and HPLC, while sensitive, are costly, labour-intensive, and unsuitable for rapid on-site testing. Here, we report green electrochemical biosensors for AA and FA detection that integrate environmentally friendly graphene with 1-pyrenebutyric acid N-hydroxysuccinamide ester (PyrNHS) on disposable screen-printed carbon electrodes (SPCEs). Graphene was synthesized via liquid-phase exfoliation in an ethanol–water system, producing few-layer structures with abundant functional groups for stable bioreceptor attachment. PyrNHS enabled noncovalent anchoring and oriented immobilization of hemoglobin (Hb) for AA and formaldehyde dehydrogenase (FDH) for FA, overcoming limitations of costly and unstable nanomaterials. The AA biosensor operated through a signal-off mechanism using differential pulse voltammetry, with a detection limit of 4.39 μM over 0–25 μM. The FA biosensor employed a signal-on enzymatic mechanism with chronoamperometry, achieving 0.02 mM detection limit across 0.1–0.6 mM. Both sensors showed high selectivity, reproducibility (RSD <1.3 %), and effective performance in instant coffee, consistent with roasting chemistry and validated by Nash assay. This work demonstrates a sustainable, low-cost, and portable platform for point-of-care (POC) carcinogen monitoring.

食品安全监测需要检测化学危害的实用策略，如丙烯酰胺（AA）和甲醛（FA），这两种物质都被列为可能的人类致癌物，饮食暴露量往往超过可容忍的限度。传统的方法包括气相色谱-质谱和高效液相色谱，虽然敏感，但成本高，劳动密集，不适合快速现场检测。在这里，我们报道了用于AA和FA检测的绿色电化学生物传感器，该传感器在一次性丝网印刷碳电极（spce）上集成了环保石墨烯和1-芘丁酸n-羟基琥珀酰胺酯（PyrNHS）。在乙醇-水体系中通过液相剥离法制备了石墨烯，制备了具有丰富官能团的少层结构，以稳定的生物受体附着。PyrNHS实现了非共价锚定和定向固定化血红蛋白（Hb）用于AA和甲醛脱氢酶（FDH）用于FA，克服了昂贵和不稳定的纳米材料的局限性。AA生物传感器采用差分脉冲伏安法，在0 ~ 25 μM范围内检测限为4.39 μM。FA生物传感器采用酶促信号机制和计时电流法，在0.1-0.6 mM范围内达到0.02 mM的检测限。两种传感器在速溶咖啡中均具有高选择性、重复性（RSD < 1.3%）和有效性能，与烘焙化学一致，并通过Nash试验验证。这项工作展示了一种可持续的、低成本的、便携式的医疗点（POC）致癌物监测平台。

{"title":"Green electrochemical biosensor for food safety monitoring of carcinogenic acrylamide and formaldehyde","authors":"Catarina Meliana , Michelle Tien Tien Tan , Hwei-San Loh , Weihua Meng , Cheng Heng Pang , Sze Shin Low","doi":"10.1016/j.bioelechem.2025.109122","DOIUrl":"10.1016/j.bioelechem.2025.109122","url":null,"abstract":"<div><div>Food safety monitoring demands practical strategies for detecting chemical hazards such as acrylamide (AA) and formaldehyde (FA), both classified as probable human carcinogens with dietary exposures often exceeding tolerable limits. Conventional methods including GC–MS and HPLC, while sensitive, are costly, labour-intensive, and unsuitable for rapid on-site testing. Here, we report green electrochemical biosensors for AA and FA detection that integrate environmentally friendly graphene with 1-pyrenebutyric acid N-hydroxysuccinamide ester (PyrNHS) on disposable screen-printed carbon electrodes (SPCEs). Graphene was synthesized via liquid-phase exfoliation in an ethanol–water system, producing few-layer structures with abundant functional groups for stable bioreceptor attachment. PyrNHS enabled noncovalent anchoring and oriented immobilization of hemoglobin (Hb) for AA and formaldehyde dehydrogenase (FDH) for FA, overcoming limitations of costly and unstable nanomaterials. The AA biosensor operated through a signal-off mechanism using differential pulse voltammetry, with a detection limit of 4.39 μM over 0–25 μM. The FA biosensor employed a signal-on enzymatic mechanism with chronoamperometry, achieving 0.02 mM detection limit across 0.1–0.6 mM. Both sensors showed high selectivity, reproducibility (RSD <1.3 %), and effective performance in instant coffee, consistent with roasting chemistry and validated by Nash assay. This work demonstrates a sustainable, low-cost, and portable platform for point-of-care (POC) carcinogen monitoring.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"168 ","pages":"Article 109122"},"PeriodicalIF":4.5,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145217502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-performance electrochemical aptasensor for detection of glypican-3 based on nitrogen-doped reduced graphene oxide-ferrocene-polyaniline nanocomposites 基于氮掺杂还原氧化石墨烯-二茂铁-聚苯胺纳米复合材料的高性能电化学感应传感器检测glyypican -3

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2025-09-29 DOI: 10.1016/j.bioelechem.2025.109127

Xuyang Chen , Lingling Fan , Kaipeng Su , Shuwei Wen , Xiaohong Tan , Yuge Liu , Xiaohua Deng , Xiangming Li , Yong Huang , Guiyin Li

Hepatocellular carcinoma (HCC) is a high-mortality malignancy that urgently requires sensitive early-stage biomarkers. Glypican-3 (GPC3), a highly specific protein biomarker for HCC, calls for efficient detection strategies. Herein, we developed a label-free electrochemical aptasensor based on nitrogen-doped reduced graphene oxide-ferrocene-polyaniline (NRGO-Fc-PANI) nanocomposites and GPC3 aptamer for GPC3 detection. The NRGO-Fc-PANI combined the large high surface area and conductivity of NRGO, high electrical conductivity and stability of PANI, and the good redox properties of Fc, which functed as in situ electrochemical redox signal indicator to monitor the electrochemical changes. Upon target binding, the formation of GPC3-aptamer complex fell off from the electrode, exposing the conductive NRGO-Fc-PANI layer and enhancing the differential pulse voltammetry (DPV) response. The aptasensor exhibited a linear detection range of 10.0–100.0 ng·mL⁻¹ (R² = 0.996) and a low limit of detection (LOD) of 2.88 ng·mL⁻¹. It demonstrated excellent selectivity toward interfering proteins, retained 70.8 % of its initial signal after 11 days of storage, and attained a recovery range of 97.0–109.5 % in human serum samples. By eliminating the need for enzymatic or fluorescent reporters, the aptasensor reduced reagent costs and operational complexity, presenting a clinically promising tool for the early diagnosis of HCC.

肝细胞癌（HCC）是一种高死亡率的恶性肿瘤，迫切需要敏感的早期生物标志物。Glypican-3 （GPC3）是HCC的高特异性蛋白质生物标志物，需要有效的检测策略。在此，我们开发了一种基于氮掺杂还原氧化石墨烯-二茂铁-聚苯胺（NRGO-Fc-PANI）纳米复合材料和GPC3适体的无标记电化学适体传感器，用于GPC3检测。NRGO-Fc-PANI结合了NRGO大的高表面积和导电性，PANI的高导电性和稳定性，以及Fc良好的氧化还原性能，可以作为现场电化学氧化还原信号指示器来监测电化学变化。靶结合后，形成的gpc3 -适体复合物从电极上脱落，暴露出导电的NRGO-Fc-PANI层，增强了差分脉冲伏安（DPV）响应。该传感器的线性检测范围为10.0 ~ 100.0 ng·mL−1 (R2 = 0.996)，最低检出限为2.88 ng·mL−1。对干扰蛋白具有良好的选择性，保存11天后，其初始信号保留率为70.8%，在人血清样品中回收率为97.0 ~ 109.5%。通过消除对酶或荧光报告的需要，该适配体传感器降低了试剂成本和操作复杂性，为HCC的早期诊断提供了一种临床有前景的工具。

{"title":"High-performance electrochemical aptasensor for detection of glypican-3 based on nitrogen-doped reduced graphene oxide-ferrocene-polyaniline nanocomposites","authors":"Xuyang Chen , Lingling Fan , Kaipeng Su , Shuwei Wen , Xiaohong Tan , Yuge Liu , Xiaohua Deng , Xiangming Li , Yong Huang , Guiyin Li","doi":"10.1016/j.bioelechem.2025.109127","DOIUrl":"10.1016/j.bioelechem.2025.109127","url":null,"abstract":"<div><div>Hepatocellular carcinoma (HCC) is a high-mortality malignancy that urgently requires sensitive early-stage biomarkers. Glypican-3 (GPC3), a highly specific protein biomarker for HCC, calls for efficient detection strategies. Herein, we developed a label-free electrochemical aptasensor based on nitrogen-doped reduced graphene oxide-ferrocene-polyaniline (NRGO-Fc-PANI) nanocomposites and GPC3 aptamer for GPC3 detection. The NRGO-Fc-PANI combined the large high surface area and conductivity of NRGO, high electrical conductivity and stability of PANI, and the good redox properties of Fc, which functed as in situ electrochemical redox signal indicator to monitor the electrochemical changes. Upon target binding, the formation of GPC3-aptamer complex fell off from the electrode, exposing the conductive NRGO-Fc-PANI layer and enhancing the differential pulse voltammetry (DPV) response. The aptasensor exhibited a linear detection range of 10.0–100.0 ng·mL<sup>−1</sup> (R<sup>2</sup> = 0.996) and a low limit of detection (LOD) of 2.88 ng·mL<sup>−1</sup>. It demonstrated excellent selectivity toward interfering proteins, retained 70.8 % of its initial signal after 11 days of storage, and attained a recovery range of 97.0–109.5 % in human serum samples. By eliminating the need for enzymatic or fluorescent reporters, the aptasensor reduced reagent costs and operational complexity, presenting a clinically promising tool for the early diagnosis of HCC.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"168 ","pages":"Article 109127"},"PeriodicalIF":4.5,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145217501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrochemical immunosensor based on sea urchin-like PdAg and Au NPs/N-C@CNTs for ultrasensitive detection of CEA 基于海胆样PdAg和Au NPs/N-C@CNTs的电化学免疫传感器用于CEA的超灵敏检测。

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2025-09-28 DOI: 10.1016/j.bioelechem.2025.109124

Yang Li , Hongzhu Yan , Zhe Zhao, Ping Wang, Feng Tang, Qingyan Chu, Zhao Yang, Shujun Wang, Qing Liu, Yueyun Li

Carcinoembryonic antigen (CEA), a clinically critical tumor biomarker, enables early cancer screening and diagnosis. Here, we describe a sandwich-structured electrochemical immunosensing platform enabling supersensitive CEA quantification, leveraging synergistic signal amplification by sea urchin-like PdAg nanostructures and Au NPs/N-C@CNTs substrates. The urchin-like morphology of PdAg endows the material additional catalytic active sites for hydrogen peroxide reduction, which has remarkable electrochemical performance. Moreover, PdAg with superior biocompatibility can effectively immobilize the secondary antibody. Polydopamine-coated carbon nanotubes are carbonized to yield nitrogen-doped carbon nanotubes (N-C@CNTs), which are bound to gold nanoparticles (Au NPs) via stable AuN bonds, thereby facilitating the subsequent binding of primary antibodies to the Au NPs. Optimized assays demonstrated a broad dynamic range (50 fg mL⁻¹–100 ng mL⁻¹) with low detection limits (1.04 fg mL⁻¹, S/N = 3), coupled with exceptional reproducibility, selectivity, and stability. This platform holds significant promise for the screening of early-stage tumor biomarkers.

癌胚抗原（CEA）是临床重要的肿瘤生物标志物，可用于早期癌症筛查和诊断。在这里，我们描述了一个三明治结构的电化学免疫传感平台，利用海胆样PdAg纳米结构和Au NPs/N-C@CNTs底物的协同信号放大，实现了超灵敏的CEA定量。PdAg的海胆状形态赋予了材料额外的过氧化氢还原催化活性位点，具有显著的电化学性能。此外，PdAg具有良好的生物相容性，可以有效地固定二抗。将多多巴胺包被的碳纳米管碳化生成氮掺杂碳纳米管（N-C@CNTs），碳纳米管通过稳定的AuN键与金纳米粒子（Au NPs）结合，从而促进了随后一抗与Au NPs的结合。优化后的分析方法具有较宽的动态范围（50 fg mL-1-100 ng mL-1），低检出限（1.04 fg mL-1, S/N = 3），以及出色的重现性、选择性和稳定性。该平台对早期肿瘤生物标志物的筛选具有重要的前景。

{"title":"Electrochemical immunosensor based on sea urchin-like PdAg and Au NPs/N-C@CNTs for ultrasensitive detection of CEA","authors":"Yang Li , Hongzhu Yan , Zhe Zhao, Ping Wang, Feng Tang, Qingyan Chu, Zhao Yang, Shujun Wang, Qing Liu, Yueyun Li","doi":"10.1016/j.bioelechem.2025.109124","DOIUrl":"10.1016/j.bioelechem.2025.109124","url":null,"abstract":"<div><div>Carcinoembryonic antigen (CEA), a clinically critical tumor biomarker, enables early cancer screening and diagnosis. Here, we describe a sandwich-structured electrochemical immunosensing platform enabling supersensitive CEA quantification, leveraging synergistic signal amplification by sea urchin-like PdAg nanostructures and Au NPs/N-C@CNTs substrates. The urchin-like morphology of PdAg endows the material additional catalytic active sites for hydrogen peroxide reduction, which has remarkable electrochemical performance. Moreover, PdAg with superior biocompatibility can effectively immobilize the secondary antibody. Polydopamine-coated carbon nanotubes are carbonized to yield nitrogen-doped carbon nanotubes (N-C@CNTs), which are bound to gold nanoparticles (Au NPs) via stable Au<img>N bonds, thereby facilitating the subsequent binding of primary antibodies to the Au NPs. Optimized assays demonstrated a broad dynamic range (50 fg mL<sup>−1</sup>–100 ng mL<sup>−1</sup>) with low detection limits (1.04 fg mL<sup>−1</sup>, S/N = 3), coupled with exceptional reproducibility, selectivity, and stability. This platform holds significant promise for the screening of early-stage tumor biomarkers.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"168 ","pages":"Article 109124"},"PeriodicalIF":4.5,"publicationDate":"2025-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145197704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hexagonally structured microbial desalination cell for bio-electrochemically mediated removal of pollutants and improved desalination of hypersaline solution. 用于生物电化学去除污染物和改进高盐溶液脱盐的六边形结构微生物脱盐电池。

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2025-09-28 DOI: 10.1016/j.bioelechem.2025.109118

Francis Kwarteng , Jingyu Huang , Prince Atta Opoku

This study introduces a new hexagonally structured multi-anode shared cathode microbial desalination cell (MASC-MDC) designed to address the limitations of traditional MDCs in handling hypersaline solutions. The hexagonal shape shortens the intermembrane distance to 2 cm, significantly reducing internal resistance (∼50 Ω) and enhancing bioelectrochemical performance. Compared to a conventional three-chamber MDC, the MASC-MDC achieved better results, including a higher open-circuit voltage (646 vs. 553 mV), faster desalination (95.71 % in five cycles vs. 94.29 % in seven), higher desalination rate (0.27 vs. 0.195 g·L⁻¹·h⁻¹), and greater maximum power density (162.2 vs. 119.2 mW·m⁻²). The system also attained effective pollutant removal with 90.05 % COD reduction. These findings demonstrate that the multi-anode shared cathode design enhances ion transport, bioenergy production, and wastewater treatment simultaneously, offering a scalable, self-powered alternative to energy-intensive desalination methods.

本研究介绍了一种新型六边形结构的多阳极共享阴极微生物海水淡化电池（MASC-MDC），旨在解决传统mdc在处理高盐溶液方面的局限性。六角形将膜间距离缩短至2 cm，显著降低了内阻（~ 50 Ω），提高了生物电化学性能。与传统的三腔室MDC相比，MASC-MDC取得了更好的效果，包括更高的开路电压（646 vs. 553 mV），更快的脱盐速度（5次循环95.71% vs. 7次94.29%），更高的脱盐率（0.27 vs. 0.195 g·L−1·h−1）和更高的最大功率密度（162.2 vs. 119.2 mW·m−2）。该系统还达到了有效的污染物去除效果，COD降低90.05%。这些发现表明，多阳极共享阴极设计可以同时增强离子传输、生物能源生产和废水处理，为能源密集型海水淡化方法提供了一种可扩展的、自供电的替代方案。

{"title":"Hexagonally structured microbial desalination cell for bio-electrochemically mediated removal of pollutants and improved desalination of hypersaline solution.","authors":"Francis Kwarteng , Jingyu Huang , Prince Atta Opoku","doi":"10.1016/j.bioelechem.2025.109118","DOIUrl":"10.1016/j.bioelechem.2025.109118","url":null,"abstract":"<div><div>This study introduces a new hexagonally structured multi-anode shared cathode microbial desalination cell (MASC-MDC) designed to address the limitations of traditional MDCs in handling hypersaline solutions. The hexagonal shape shortens the intermembrane distance to 2 cm, significantly reducing internal resistance (∼50 Ω) and enhancing bioelectrochemical performance. Compared to a conventional three-chamber MDC, the MASC-MDC achieved better results, including a higher open-circuit voltage (646 vs. 553 mV), faster desalination (95.71 % in five cycles vs. 94.29 % in seven), higher desalination rate (0.27 vs. 0.195 g·L<sup>−1</sup>·h<sup>−1</sup>), and greater maximum power density (162.2 vs. 119.2 mW·m<sup>−2</sup>). The system also attained effective pollutant removal with 90.05 % COD reduction. These findings demonstrate that the multi-anode shared cathode design enhances ion transport, bioenergy production, and wastewater treatment simultaneously, offering a scalable, self-powered alternative to energy-intensive desalination methods.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"168 ","pages":"Article 109118"},"PeriodicalIF":4.5,"publicationDate":"2025-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145217500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cascade redox cycling amplification enabled by COF-confined Co3O4 for enhanced immunosensing 通过cof约束的Co3O4实现级联氧化还原循环扩增，增强免疫感知。

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2025-09-25 DOI: 10.1016/j.bioelechem.2025.109125

Jie Zhang , Ning Chen , Shujun Wang , Xiaofei Sun , Shuangna Wang , Ping Wang , Yueyun Li , Qing Liu , Jongnam Park , Feng Tang

Herein, an enzyme-free and highly efficient sandwich-type electrochemical immunosensor for cTnI detection was developed using covalent organic framework (COF) confined Co₃O₄ nanoparticles (NPs) as the signal probe and enhancing the sensitivity with electrochemical-chemical-chemical (ECC) redox cycle amplification (RCA) strategy. The multifunctional COF, with high surface area and rich nitrogen, serves not only as a substrate material but also as a scaffold for Co²⁺ entrapment, enabling the confined growth and uniform distribution of ultrafine Co₃O₄ NPs as signal amplification platform, thereby providing abundant catalytic active sites for ECC redox cycling reactions. COF confined Co₃O₄ NPs with variable valence states (Co³⁺/Co²⁺) serve as a redox-active electrode material that can enhance the current signal substantially. The ECC redox cycle is triggered by the redox reaction between Co³⁺ at the electrode and electroactive hydroquinone (HQ), while HQ was regenerated by the reducing agent tris (2-carboxyethyl) phosphine (TCEP), resulting in a significant amplification of the current signal for cTnI analysis. The constructed immunosensor exhibited excellent performance with a wide linear range from 1 fg mL⁻¹ to 100 ng mL⁻¹, and a low detection limit of 0.88 fg mL⁻¹. Furthermore, the immunosensor successfully applied to detected cTnI in human serum, proving its clinical potential.

本文以共价有机框架（COF）约束的Co3O4纳米颗粒（NPs）为信号探针，采用电化学-化学-化学（ECC）氧化还原循环扩增（RCA）策略提高灵敏度，研制了一种无酶、高效的三明治型cTnI检测电化学免疫传感器。具有高表面积和富氮的多功能COF不仅可以作为底物材料，还可以作为Co2+捕集的支架，使超细Co3O4 NPs受限生长和均匀分布成为信号放大平台，从而为ECC氧化还原循环反应提供丰富的催化活性位点。具有可变价态（Co3+/Co2+）的COF约束Co3O4 NPs作为氧化还原活性电极材料，可以显著增强电流信号。ECC的氧化还原循环由电极处的Co3+与电活性对苯二酚（HQ）的氧化还原反应触发，而HQ则由还原剂三（2-羧基乙基）膦（TCEP）再生，导致用于cTnI分析的电流信号显著放大。所构建的免疫传感器具有良好的性能，线性范围为1 ~ 100 ng mL-1，检出限低至0.88 fg mL-1。此外，该免疫传感器成功应用于人血清中cTnI的检测，证明了其临床应用潜力。

{"title":"Cascade redox cycling amplification enabled by COF-confined Co3O4 for enhanced immunosensing","authors":"Jie Zhang , Ning Chen , Shujun Wang , Xiaofei Sun , Shuangna Wang , Ping Wang , Yueyun Li , Qing Liu , Jongnam Park , Feng Tang","doi":"10.1016/j.bioelechem.2025.109125","DOIUrl":"10.1016/j.bioelechem.2025.109125","url":null,"abstract":"<div><div>Herein, an enzyme-free and highly efficient sandwich-type electrochemical immunosensor for cTnI detection was developed using covalent organic framework (COF) confined Co<sub>3</sub>O<sub>4</sub> nanoparticles (NPs) as the signal probe and enhancing the sensitivity with electrochemical-chemical-chemical (ECC) redox cycle amplification (RCA) strategy. The multifunctional COF, with high surface area and rich nitrogen, serves not only as a substrate material but also as a scaffold for Co<sup>2+</sup> entrapment, enabling the confined growth and uniform distribution of ultrafine Co<sub>3</sub>O<sub>4</sub> NPs as signal amplification platform, thereby providing abundant catalytic active sites for ECC redox cycling reactions. COF confined Co<sub>3</sub>O<sub>4</sub> NPs with variable valence states (Co<sup>3+</sup>/Co<sup>2+</sup>) serve as a redox-active electrode material that can enhance the current signal substantially. The ECC redox cycle is triggered by the redox reaction between Co<sup>3+</sup> at the electrode and electroactive hydroquinone (HQ), while HQ was regenerated by the reducing agent tris (2-carboxyethyl) phosphine (TCEP), resulting in a significant amplification of the current signal for cTnI analysis. The constructed immunosensor exhibited excellent performance with a wide linear range from 1 fg mL<sup>−1</sup> to 100 ng mL<sup>−1</sup>, and a low detection limit of 0.88 fg mL<sup>−1</sup>. Furthermore, the immunosensor successfully applied to detected cTnI in human serum, proving its clinical potential.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"168 ","pages":"Article 109125"},"PeriodicalIF":4.5,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145197689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent advances and perspectives on N2 fixation by microbial bioelectrochemical systems 微生物电化学系统固氮研究进展与展望

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2025-09-24 DOI: 10.1016/j.bioelechem.2025.109123

Axel Rous , James A. Behan , Elie Desmond-Le Quéméner , Nicolas Bernet , Eric Trably , Frédéric Gloaguen , Frédéric Barrière

This review covers recent advances in the fixing of dinitrogen in microbial bioelectrochemical systems (BES) where bacteria release or accept electron to/from electrodes for their respiratory metabolism, either directly or indirectly. We discuss how BES may be interesting platforms for producing ammonium or biomass from N₂ fixation. The potential for N₂-fixation in BES is first discussed with a focus on possible metabolism and different mechanism that may lead to an increase of fixed dinitrogen. We then review recent examples where dinitrogen is fixed at the cathodes of BES, either by pure cultures of hydrogenotrophic and/or diazotrophic bacteria using cathodic H₂ or reduced redox mediators as the electron, or by mixed enriched consortia. A section is then devoted to the special case of nitrogen fixation at anodic microbial electrode where organic matter oxidation also occurs. Finally, a comparison of the reported current performance of nitrogen fixation in BES with other biotic (anerobic digestion) or abiotic (Haber-Bosch process, electrochemical N₂ reduction) is provided together with a perspective on possible optimization and application of this emerging microbial electrochemical and technological process.

本文综述了微生物电化学系统（BES）中二氮固定的最新进展，细菌直接或间接地向电极释放或接受电子以进行呼吸代谢。我们讨论了BES如何可能成为从氮气固定中生产铵或生物质的有趣平台。本文首先讨论了BES中固氮的潜力，重点讨论了可能导致固氮增加的代谢和不同机制。然后，我们回顾了最近的例子，其中二氮固定在BES的阴极上，通过纯培养的氢营养型和/或重氮营养型细菌，使用阴极H2或还原氧化还原介质作为电子，或通过混合富集菌群。然后一节专门讨论在阳极微生物电极上固氮的特殊情况，其中有机物氧化也发生。最后，将目前报道的BES固氮性能与其他生物（厌氧消化）或非生物（Haber-Bosch工艺，电化学N2还原）进行了比较，并对这一新兴的微生物电化学和技术过程的可能优化和应用进行了展望。

{"title":"Recent advances and perspectives on N2 fixation by microbial bioelectrochemical systems","authors":"Axel Rous , James A. Behan , Elie Desmond-Le Quéméner , Nicolas Bernet , Eric Trably , Frédéric Gloaguen , Frédéric Barrière","doi":"10.1016/j.bioelechem.2025.109123","DOIUrl":"10.1016/j.bioelechem.2025.109123","url":null,"abstract":"<div><div>This review covers recent advances in the fixing of dinitrogen in microbial bioelectrochemical systems (BES) where bacteria release or accept electron to/from electrodes for their respiratory metabolism, either directly or indirectly. We discuss how BES may be interesting platforms for producing ammonium or biomass from N<sub>2</sub> fixation. The potential for N<sub>2</sub>-fixation in BES is first discussed with a focus on possible metabolism and different mechanism that may lead to an increase of fixed dinitrogen. We then review recent examples where dinitrogen is fixed at the cathodes of BES, either by pure cultures of hydrogenotrophic and/or diazotrophic bacteria using cathodic H<sub>2</sub> or reduced redox mediators as the electron, or by mixed enriched consortia. A section is then devoted to the special case of nitrogen fixation at anodic microbial electrode where organic matter oxidation also occurs. Finally, a comparison of the reported current performance of nitrogen fixation in BES with other biotic (anerobic digestion) or abiotic (Haber-Bosch process, electrochemical N<sub>2</sub> reduction) is provided together with a perspective on possible optimization and application of this emerging microbial electrochemical and technological process.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"168 ","pages":"Article 109123"},"PeriodicalIF":4.5,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145155001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Magnetized electrochemically active bacteria-based whole-cell biosensors for real-time sensing of water toxicity 用于水毒性实时传感的磁化电化学活性细菌全细胞生物传感器

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2025-09-23 DOI: 10.1016/j.bioelechem.2025.109119

Hongyu Zhao , Yanhong Ge , Jing Wu , Bo Cao , Yue Yi , Beizhen Xie , Hong Liu

Real-time sensing of water toxicity is essential for environmental health monitoring. However, devising an electrochemically active biofilm-based biosensor for water toxicity assaying usually requires cumbersome culture techniques to immobilize electrochemically active bacteria (EAB) on the electrode, which results in poor timeliness of water quality early-warning. Herein, we developed magnetized electrochemically active bacteria (MEAB)-based whole-cell biosensors (WCBs) for real-time sensing of water toxicity. The results showed that artificial MEAB biofilm could be magnetically constructed in one step within 5 s, which greatly simplified the fabrication process of artificial electrochemically active biofilm. By correlating quantifiable bioelectrical signal with MEAB cell activity, the MEAB-based WCBs enabled detection of Hg²⁺, trichloroacetic acid (TCAA), avermectin (AVM), Cr⁶⁺, and chlortetracycline hydrochloride (CTC) in synthetic water samples within 30 min. The estimated detection limit for Hg²⁺, TCAA, AVM, Cr⁶⁺, and CTC reached 50.4 ± 1.6, 54.7 ± 1.5, 62.8 ± 2.0, 66.8 ± 1.6, and 73.3 ± 2.2 μg L⁻¹ with optimal biomass, respectively. As proof-of-concept applications, the MEAB-based WCBs not only achieved accurate detection of 0.1 mg L⁻¹ toxicants in real water samples but also successfully sensed comprehensive toxicity of agricultural wastewater within 30 min. This study provides a new strategy for real-time sensing of water toxicity.

水毒性的实时监测是环境健康监测的必要条件。然而，设计用于水毒性分析的电化学活性生物膜生物传感器通常需要繁琐的培养技术来将电化学活性细菌（EAB）固定在电极上，这导致水质预警的及时性较差。在此，我们开发了基于磁化电化学活性细菌（MEAB）的全细胞生物传感器（WCBs），用于实时检测水毒性。结果表明，MEAB人工生物膜可在5 s内一步磁性构建完成，大大简化了人工电化学活性生物膜的制备过程。通过将可量化的生物电信号与MEAB细胞活性相关联，基于MEAB的wcb能够在30分钟内检测合成水样中的Hg2+、三氯乙酸（TCAA）、阿维菌素（AVM）、Cr6+和盐酸氯四环素（CTC）。在最佳生物量条件下，Hg2+、TCAA、AVM、Cr6+和CTC的检出限分别为50.4±1.6、54.7±1.5、62.8±2.0、66.8±1.6和73.3±2.2 μg L−1。作为概念验证应用，基于meab的wcb不仅实现了对真实水样中0.1 mg L−1毒物的准确检测，而且在30分钟内成功地检测了农业废水的综合毒性。本研究为水毒性实时监测提供了一种新的方法。

{"title":"Magnetized electrochemically active bacteria-based whole-cell biosensors for real-time sensing of water toxicity","authors":"Hongyu Zhao , Yanhong Ge , Jing Wu , Bo Cao , Yue Yi , Beizhen Xie , Hong Liu","doi":"10.1016/j.bioelechem.2025.109119","DOIUrl":"10.1016/j.bioelechem.2025.109119","url":null,"abstract":"<div><div>Real-time sensing of water toxicity is essential for environmental health monitoring. However, devising an electrochemically active biofilm-based biosensor for water toxicity assaying usually requires cumbersome culture techniques to immobilize electrochemically active bacteria (EAB) on the electrode, which results in poor timeliness of water quality early-warning. Herein, we developed magnetized electrochemically active bacteria (MEAB)-based whole-cell biosensors (WCBs) for real-time sensing of water toxicity. The results showed that artificial MEAB biofilm could be magnetically constructed in one step within 5 s, which greatly simplified the fabrication process of artificial electrochemically active biofilm. By correlating quantifiable bioelectrical signal with MEAB cell activity, the MEAB-based WCBs enabled detection of Hg<sup>2+</sup>, trichloroacetic acid (TCAA), avermectin (AVM), Cr<sup>6+</sup>, and chlortetracycline hydrochloride (CTC) in synthetic water samples within 30min. The estimated detection limit for Hg<sup>2+</sup>, TCAA, AVM, Cr<sup>6+</sup>, and CTC reached 50.4 ± 1.6, 54.7 ± 1.5, 62.8 ± 2.0, 66.8 ± 1.6, and 73.3 ± 2.2 μg L<sup>−1</sup> with optimal biomass, respectively. As proof-of-concept applications, the MEAB-based WCBs not only achieved accurate detection of 0.1 mg L<sup>−1</sup> toxicants in real water samples but also successfully sensed comprehensive toxicity of agricultural wastewater within 30 min. This study provides a new strategy for real-time sensing of water toxicity.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"168 ","pages":"Article 109119"},"PeriodicalIF":4.5,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145154998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Highly water-retaining conductive hydrogels based on multi-crosslinked networks for ultrasensitive sensing platform 基于多交联网络的高保水导电水凝胶用于超灵敏传感平台

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2025-09-23 DOI: 10.1016/j.bioelechem.2025.109120

Jiaqing Wang , Youyu Li , Ning Li , Hongliang Han , Zhanfang Ma , Haijun Yang

Hydrogels are widely used in electrochemical sensors due to their unique properties, but their conductivity, influenced by water-content, is highly susceptible to external environment. Therefore, enhancing the water-retention of hydrogels while ensuring stable conductivity and analytical performance is crucial for broadening their application. In this work, a novel polyacrylamide/bacterial cellulose/sulfobetaine methacrylate/sodium alginate composite hydrogel (PBSS)-based multi-crosslinked network hydrogel was designed. The water retention of the PBSS hydrogel was improved by a factor of 1.5 compared to the unreinforced polyacrylamide (PAM) hydrogel (Water loss of hydrogel exposed for 12 h at 37 °C). With the water retention properties of itself, the PBSS hydrogel retained 86 % of its initial conductivity after 12 h of exposure at 60 °C, whereas the PAM hydrogel not only exhibited poor initial conductivity but also lost up to 47 % of its conductivity. PBSS hydrogels were designed as sensing platforms and CaCO₃ spheres were designed as immunoprobes. Ca²⁺ released by the probe rivals Ni²⁺ for the signaling substance on the substrate, enabling the quantification of the target analyte. The sensor exhibited excellent analytical performance and maintained stable performance after four days of storage at 37 °C, offering a promising approach to enhance hydrogel sensor stability for clinical applications.

水凝胶因其独特的性能被广泛应用于电化学传感器中，但其电导率受水含量的影响，极易受外界环境的影响。因此，在保证稳定的电导率和分析性能的同时提高水凝胶的保水性是扩大其应用的关键。本文设计了一种新型的聚丙烯酰胺/细菌纤维素/甲基丙烯酸磺基甜菜碱/海藻酸钠复合水凝胶（PBSS）。与未增强的聚丙烯酰胺（PAM）水凝胶相比，PBSS水凝胶的保水性提高了1.5倍（水凝胶在37℃下暴露12小时的失水）。由于PBSS水凝胶本身具有保水性，在60°C下暴露12小时后，其初始电导率仍保持86%，而PAM水凝胶不仅表现出较差的初始电导率，而且电导率损失高达47%。设计PBSS水凝胶作为传感平台，CaCO3球作为免疫探针。探针释放的Ca2+与底物上的信号物质Ni2+竞争，使目标分析物的量化成为可能。该传感器表现出优异的分析性能，并在37°C下保存4天后保持稳定的性能，为提高水凝胶传感器的稳定性提供了一种有前途的方法。

{"title":"Highly water-retaining conductive hydrogels based on multi-crosslinked networks for ultrasensitive sensing platform","authors":"Jiaqing Wang , Youyu Li , Ning Li , Hongliang Han , Zhanfang Ma , Haijun Yang","doi":"10.1016/j.bioelechem.2025.109120","DOIUrl":"10.1016/j.bioelechem.2025.109120","url":null,"abstract":"<div><div>Hydrogels are widely used in electrochemical sensors due to their unique properties, but their conductivity, influenced by water-content, is highly susceptible to external environment. Therefore, enhancing the water-retention of hydrogels while ensuring stable conductivity and analytical performance is crucial for broadening their application. In this work, a novel polyacrylamide/bacterial cellulose/sulfobetaine methacrylate/sodium alginate composite hydrogel (PBSS)-based multi-crosslinked network hydrogel was designed. The water retention of the PBSS hydrogel was improved by a factor of 1.5 compared to the unreinforced polyacrylamide (PAM) hydrogel (Water loss of hydrogel exposed for 12 h at 37 °C). With the water retention properties of itself, the PBSS hydrogel retained 86 % of its initial conductivity after 12 h of exposure at 60 °C, whereas the PAM hydrogel not only exhibited poor initial conductivity but also lost up to 47 % of its conductivity. PBSS hydrogels were designed as sensing platforms and CaCO<sub>3</sub> spheres were designed as immunoprobes. Ca<sup>2+</sup> released by the probe rivals Ni<sup>2+</sup> for the signaling substance on the substrate, enabling the quantification of the target analyte. The sensor exhibited excellent analytical performance and maintained stable performance after four days of storage at 37 °C, offering a promising approach to enhance hydrogel sensor stability for clinical applications.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"168 ","pages":"Article 109120"},"PeriodicalIF":4.5,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145154999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0