Pub Date : 2024-12-09DOI: 10.1016/j.bioelechem.2024.108881
Xiaoying Cui , Ke Zhao , Zhengquan Qu , Xipeng Chao , Lingling Xie , Hanyu Chen , Baoshan He , Baozhong Zhang
In this work, an efficient sensing platform deoxynivalenol (DON) detection was constructed through monitoring the current change of a competitive mechanism triggered by DON, leading the signal label detached from the electrode surface by square-wave voltammetry using thionine (Thi) as a redox indicator. The complementary strand of aptamer (cDNA) and Thi were loaded onto Fe/Ni bimetallic metal–organic framework loaded with sliver nanoparticles (AgNPs@FeNi-MOF) to construct AgNPs@FeNi-MOF/cDNA/Thi signal probes. In the presence of DON, the aptamer sequence was more predisposed to form an aptamer-DON complex, resulting in the displacement of the cDNA. The signal probe was subsequently released, leading to a decrease in the signal intensity of Thi. Notably, AgNPs@FeNi-MOF has a larger electroactive specific surface area and is able to load more cDNA and thi, which can amplify the signal. Under the optimal experimental conditions, the developed sensor exhibits a good linear response in the range of 1 × 10−2 to 1 × 104 pg/mL, with a limit of detection (LOD) of 5.68 fg/mL and has good selectivity, reproducibility and stability.
{"title":"Selective aptasensor of deoxynivalenol based on dual signal enhancement of thionine electrochemistry using silver nanoparticle-loaded label at gold nanoparticle-loaded electrodes","authors":"Xiaoying Cui , Ke Zhao , Zhengquan Qu , Xipeng Chao , Lingling Xie , Hanyu Chen , Baoshan He , Baozhong Zhang","doi":"10.1016/j.bioelechem.2024.108881","DOIUrl":"10.1016/j.bioelechem.2024.108881","url":null,"abstract":"<div><div>In this work, an efficient sensing platform deoxynivalenol (DON) detection was constructed through monitoring the current change of a competitive mechanism triggered by DON, leading the signal label detached from the electrode surface by square-wave voltammetry using thionine (Thi) as a redox indicator. The complementary strand of aptamer (cDNA) and Thi were loaded onto Fe/Ni bimetallic metal–organic framework loaded with sliver nanoparticles (AgNPs@FeNi-MOF) to construct AgNPs@FeNi-MOF/cDNA/Thi signal probes. In the presence of DON, the aptamer sequence was more predisposed to form an aptamer-DON complex, resulting in the displacement of the cDNA. The signal probe was subsequently released, leading to a decrease in the signal intensity of Thi. Notably, AgNPs@FeNi-MOF has a larger electroactive specific surface area and is able to load more cDNA and thi, which can amplify the signal. Under the optimal experimental conditions, the developed sensor exhibits a good linear response in the range of 1 × 10<sup>−2</sup> to 1 × 10<sup>4</sup> pg/mL, with a limit of detection (LOD) of 5.68 fg/mL and has good selectivity, reproducibility and stability.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"163 ","pages":"Article 108881"},"PeriodicalIF":4.8,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142805771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The folate receptor (FR) is a well-known biomarker that is overexpressed in many cancer cells, making it a valuable target for cancer diagnostics and therapeutic strategies. However, identifying cancer biomarkers remains a challenge due to factors such as lengthy procedures, high costs, and low sensitivity. This study presents the development of a novel, cost-effective biosensor designed for the detection of FR. To overcome the limitations of traditional immunological methods, which rely on antigen–antibody interactions, we utilized a charge-based affinity approach. Folic acid (FA) was conjugated with poly (diallyl dimethylammonium chloride) (PDDA) using an EDC-NHS linker on the surface of multi-walled carbon nanotubes. The biosensor enabled electrochemical detection of FR through differential pulse voltammetry (DPV), achieving an impressive detection limit of 1.6 pg/mL and a dynamic range of 1–10,000 ng/mL. Additionally, the biosensor exhibited excellent stability (30 days), high selectivity, and repeatability (RSD = 3.14 %, n = 5). This work presents a promising strategy for developing ligand-receptor-based biosensors. It paves the way for future applications in cancer diagnostics and biosystem interfaces, offering high performance and practical advantages.
叶酸受体（FR）是一种众所周知的生物标志物，在许多癌细胞中过表达，使其成为癌症诊断和治疗策略的有价值靶点。然而，由于程序漫长、成本高、灵敏度低等因素，识别癌症生物标志物仍然是一个挑战。本研究提出了一种新型的、具有成本效益的用于FR检测的生物传感器的开发。为了克服传统免疫学方法依赖抗原-抗体相互作用的局限性，我们利用了基于电荷的亲和方法。采用EDC-NHS连接剂在多壁碳纳米管表面将叶酸（FA）与聚二烯丙基二甲基氯化铵（PDDA）偶联。该生物传感器通过差分脉冲伏安法（DPV）实现了FR的电化学检测，检测限为1.6 pg/mL，动态范围为1-10,000 ng/mL。此外，该生物传感器表现出优异的稳定性（30天）、高选择性和可重复性（RSD = 3.14%, n = 5）。这项工作为开发基于配体受体的生物传感器提供了一个有前途的策略。它为癌症诊断和生物系统接口的未来应用铺平了道路，提供了高性能和实用的优势。
{"title":"Label-free electrochemical assessment of human serum and cancer cells to determine the folate receptor cancer biomarker","authors":"Chandrababu Rejeeth , Rafa Almeer , Alok Sharma , Nipun Babu Varukattu","doi":"10.1016/j.bioelechem.2024.108883","DOIUrl":"10.1016/j.bioelechem.2024.108883","url":null,"abstract":"<div><div>The folate receptor (FR) is a well-known biomarker that is overexpressed in many cancer cells, making it a valuable target for cancer diagnostics and therapeutic strategies. However, identifying cancer biomarkers remains a challenge due to factors such as lengthy procedures, high costs, and low sensitivity. This study presents the development of a novel, cost-effective biosensor designed for the detection of FR. To overcome the limitations of traditional immunological methods, which rely on antigen–antibody interactions, we utilized a charge-based affinity approach. Folic acid (FA) was conjugated with poly (diallyl dimethylammonium chloride) (PDDA) using an EDC-NHS linker on the surface of multi-walled carbon nanotubes. The biosensor enabled electrochemical detection of FR through differential pulse voltammetry (DPV), achieving an impressive detection limit of 1.6 pg/mL and a dynamic range of 1–10,000 ng/mL. Additionally, the biosensor exhibited excellent stability (30 days), high selectivity, and repeatability (RSD = 3.14 %, n = 5). This work presents a promising strategy for developing ligand-receptor-based biosensors. It paves the way for future applications in cancer diagnostics and biosystem interfaces, offering high performance and practical advantages.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"163 ","pages":"Article 108883"},"PeriodicalIF":4.8,"publicationDate":"2024-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142890799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-06DOI: 10.1016/j.bioelechem.2024.108878
Tomohiro Komatsu , Rino Uejima , Hiroaki Sakamoto
In recent years, wearable devices have undergone remarkable developments. These can easily help us obtain useful information such as that related to our health. However, most devices require a power supply. This limits the utilization of portability and facilities. This can lead to dangerous situations for people who require immediate measurement of their condition. Therefore, novel wearable devices that do not need a power supply or generate power themselves are desirable. Therefore, triboelectric nanogenerators (TENG) have attracted considerable attention as renewable energy sources. In this study, we focused on using the TENG technique in wearable devices, particularly biosensors. An enzyme-modified TENG biosensor for glucose detection was constructed and evaluated. As a characteristic of our glucose biosensor, a fiber brush made nylon and fluorinated ethylene‐propylene copolymer fibers was used to drive the TENG. Using chemical fibers, glucose can be detected from various contact directions. Glucose was detected sensitively by modifying the TENG with glucose oxidase (GOx) and polyaniline (PANI) that is an emeraldine base. The resulting biosensor showed better substrate specificity for glucose than for lactic acid. Overall, the proposed enzyme-modified B-TENG can be utilized as a wearable biosensor in the near future.
{"title":"Investigation of a self-powered biosensor using a brush-based triboelectric nanogenerator and an enzymatic reaction","authors":"Tomohiro Komatsu , Rino Uejima , Hiroaki Sakamoto","doi":"10.1016/j.bioelechem.2024.108878","DOIUrl":"10.1016/j.bioelechem.2024.108878","url":null,"abstract":"<div><div>In recent years, wearable devices have undergone remarkable developments. These can easily help us obtain useful information such as that related to our health. However, most devices require a power supply. This limits the utilization of portability and facilities. This can lead to dangerous situations for people who require immediate measurement of their condition. Therefore, novel wearable devices that do not need a power supply or generate power themselves are desirable. Therefore, triboelectric nanogenerators (TENG) have attracted considerable attention as renewable energy sources. In this study, we focused on using the TENG technique in wearable devices, particularly biosensors. An enzyme-modified TENG biosensor for glucose detection was constructed and evaluated. As a characteristic of our glucose biosensor, a fiber brush made nylon and fluorinated ethylene‐propylene copolymer fibers was used to drive the TENG. Using chemical fibers, glucose can be detected from various contact directions. Glucose was detected sensitively by modifying the TENG with glucose oxidase (GOx) and polyaniline (PANI) that is an emeraldine base. The resulting biosensor showed better substrate specificity for glucose than for lactic acid. Overall, the proposed enzyme-modified B-TENG can be utilized as a wearable biosensor in the near future.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"163 ","pages":"Article 108878"},"PeriodicalIF":4.8,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142805770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-06DOI: 10.1016/j.bioelechem.2024.108882
Linghui Tang , Yufeng Huang , Yue Wang , Jifan Zhao , Huiyong Lian , Yan Dong , Zhiqiang Zhang , Yasushi Hasebe
Highly stretchable, adhesive and conductive triblock hydrogel was synthesized and utilized as a flexible and stable bioelectrocatalytic sensing layer of enzyme-based amperometric glucose biosensor. The hydrogel was prepared through one-pot polymerization of 2-acrylamido-2-methyl-1-propanesulfonic acid, methacrylamide, and hydroxyethyl methacrylate. The physical and chemical properties of the hydrogel were characterized with X-ray diffraction, scanning electron microscopy, X-ray photoelectron spectroscopy, Fourier transform infrared spectroscopy and electrochemical techniques. Glucose oxidase (GOx) and chitosan (CTS) embedded hydrogel was drop-coated on glassy carbon electrode (GCE) and screen printed graphite electrode (SPGE). The resulting GOx/CTS/hydrogel-GCE and GOx/CTS/hydrogel-SPGE exhibited excellent mediated bioelectrocatalytic oxidation current for glucose. The calibration curve of glucose by the GOx/CTS/hydrogel-GCE showed the linear range from 0.25 to 15 mM with the sensitivity of 27.0 µA mM−1 cm−2. This GOx/CTS/hydrogel-based sensing layer coated on the SPGE was stable against bending, and the response to glucose was almost same irrespective of the bending angles (0, 30, 60, and 90 degree). In addition, the response to glucose was not interfered by various organic and inorganic interfering species, allowed to detect glucose in goat serum. Furthermore, the GOx/CTS/hydrogel-GCE kept its original activity of 99.64 % during 30 days’ storage under dry state in refrigerator.
{"title":"Highly stretchable, adhesive and conductive hydrogel for flexible and stable bioelectrocatalytic sensing layer of enzyme-based amperometric glucose biosensor","authors":"Linghui Tang , Yufeng Huang , Yue Wang , Jifan Zhao , Huiyong Lian , Yan Dong , Zhiqiang Zhang , Yasushi Hasebe","doi":"10.1016/j.bioelechem.2024.108882","DOIUrl":"10.1016/j.bioelechem.2024.108882","url":null,"abstract":"<div><div>Highly stretchable, adhesive and conductive triblock hydrogel was synthesized and utilized as a flexible and stable bioelectrocatalytic sensing layer of enzyme-based amperometric glucose biosensor. The hydrogel was prepared through one-pot polymerization of 2-acrylamido-2-methyl-1-propanesulfonic acid, methacrylamide, and hydroxyethyl methacrylate. The physical and chemical properties of the hydrogel were characterized with X-ray diffraction, scanning electron microscopy, X-ray photoelectron spectroscopy, Fourier transform infrared spectroscopy and electrochemical techniques. Glucose oxidase (GOx) and chitosan (CTS) embedded hydrogel was drop-coated on glassy carbon electrode (GCE) and screen printed graphite electrode (SPGE). The resulting GOx/CTS/hydrogel-GCE and GOx/CTS/hydrogel-SPGE exhibited excellent mediated bioelectrocatalytic oxidation current for glucose. The calibration curve of glucose by the GOx/CTS/hydrogel-GCE showed the linear range from 0.25 to 15 mM with the sensitivity of 27.0 µA mM<sup>−1</sup> cm<sup>−2</sup>. This GOx/CTS/hydrogel-based sensing layer coated on the SPGE was stable against bending, and the response to glucose was almost same irrespective of the bending angles (0, 30, 60, and 90 degree). In addition, the response to glucose was not interfered by various organic and inorganic interfering species, allowed to detect glucose in goat serum. Furthermore, the GOx/CTS/hydrogel-GCE kept its original activity of 99.64 % during 30 days’ storage under dry state in refrigerator.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"163 ","pages":"Article 108882"},"PeriodicalIF":4.8,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142821536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-05DOI: 10.1016/j.bioelechem.2024.108862
Yuning Zhao , Xiaojing Cui , Yilong Wang , Zhendong Wang , Xiaofei Li , Ying Wang , Huaixia Yang , Yanju Liu , Mingsan Miao
Cardiac troponin I (cTnI) is known to be among the prominent diagnostic bio-marker for acute myocardial infarction (AMI). In this paper, we proposed an electrochemical aptasensor with nanomaterial MoS2/CuS-Au as the substrate material and perillaldehyde (PA) as the surface-initiated reversible addition fragmentation chain transfer (SI-RAFT) polymerization monomer by mediating the in-situ deposition of silver metal on the electrode surface for ultrasensitive detection of cTnI. The substrate material MoS2/CuS-Au not only accelerated the rate transfer between electron, but also provided more active sites for aptamers introduction. The perillaldehyde (PA) monomers were modified onto the electrode by surface-initiated reversible addition fragmentation chain transfer polymerization (SI-RAFT), and the signal was amplified in one step by reducing silver ions to metallic silver deposited in situ on the upper of the electrode surface. Under optimum reaction conditions, this aptasensor achieved a detection limit down to 10.83 fg·mL−1. The proposed aptasensor without additional natural enzymes or biological tags, had a promising potential for use in the diagnosis of early acute myocardial infarction (AMI).
{"title":"Enhanced electrochemical aptasensor integrating MoS2/CuS-Au and SI-RAFT for dual signal amplification in cTnI detection","authors":"Yuning Zhao , Xiaojing Cui , Yilong Wang , Zhendong Wang , Xiaofei Li , Ying Wang , Huaixia Yang , Yanju Liu , Mingsan Miao","doi":"10.1016/j.bioelechem.2024.108862","DOIUrl":"10.1016/j.bioelechem.2024.108862","url":null,"abstract":"<div><div>Cardiac troponin I (cTnI) is known to be among the prominent diagnostic bio-marker for acute myocardial infarction (AMI). In this paper, we proposed an electrochemical aptasensor with nanomaterial MoS<sub>2</sub>/CuS-Au as the substrate material and perillaldehyde (PA) as the surface-initiated reversible addition fragmentation chain transfer (SI-RAFT) polymerization monomer by mediating the in-situ deposition of silver metal on the electrode surface for ultrasensitive detection of cTnI. The substrate material MoS<sub>2</sub>/CuS-Au not only accelerated the rate transfer between electron, but also provided more active sites for aptamers introduction. The perillaldehyde (PA) monomers were modified onto the electrode by surface-initiated reversible addition fragmentation chain transfer polymerization (SI-RAFT), and the signal was amplified in one step by reducing silver ions to metallic silver deposited in situ on the upper of the electrode surface. Under optimum reaction conditions, this aptasensor achieved a detection limit down to 10.83 fg·mL<sup>−1</sup>. The proposed aptasensor without additional natural enzymes or biological tags, had a promising potential for use in the diagnosis of early acute myocardial infarction (AMI).</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"163 ","pages":"Article 108862"},"PeriodicalIF":4.8,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142823503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Traumatic brain injury (TBI) induces neurogenerative disorders affecting severely daily human activities and early diagnosis is a critical requirement for prevention and cure. Here, we induced TBI formation in the Zebra fish, a model organism, by both mechanical (ultrasonic cleaner generated convulsive shock, UGCS) and chemical (pentylenetetrazol, PTZ) methods. The TBI induced cellular and neuronal changes are monitored by measuring the activities of the indicator biomarkers viz., superoxide anion (O2−) and glutamate by electrochemical techniques. For this, the α-lipoic acid (α-LA, LA) functionalized gold-silver (LA-Au/Ag) is used as an electrochemical sensor to diagnose the presence of these markers in physiological phosphate buffer saline (PBS, pH 7.4), 0.1 M KCl solutions and in TBI tissues. While the oxidation of glutamate is observed in the potential window 0.2–0.5 V, the metal mediated oxidation of O2− is observed at the potential window 0.6–1.0 V. The sensor showed good linear ranges for O2− (from 4 to 48 μM with the LOD of 4 μM for the O2− detection) and glutamate (from 20 to 130 μM with the LOD 19 μM). The TBI tissue modified electrode showed lower resistance than the normal brain tissue ((NBT), as control) due to the presence of higher amount of O2− and occurrence of Fenton’s and Heber-Weise’s reactions in the presence of [Fe(CN)6]3−/4−. For theragnostic application, the LA-Au/Ag nanoparticles is delivered into the UGCS and PTZ treated Zebrafish and electrochemical signal changes are monitored by cyclic voltammetry and impedance spectroscopy. Electrochemical data further corroborated with the activities of superoxide dismutase (SOD), Catalase (CAT) and lipid peroxidase (MDA) in parallel. The developed method of electrochemical sensing of TBI may provide alternative for the early TBI diagnosis and therapeutics for the prevention of TBI.
{"title":"In-vitro sensing of traumatic brain tissue by electrochemical impedance for diagnosis and therapeutic","authors":"Sebastinbaskar Aniu Lincy , Yesurajan Allwin Richard , Jeyaraj Jeyavani , Baskaralingam Vaseeharan , Venkataraman Dharuman","doi":"10.1016/j.bioelechem.2024.108871","DOIUrl":"10.1016/j.bioelechem.2024.108871","url":null,"abstract":"<div><div>Traumatic brain injury (TBI) induces neurogenerative disorders affecting severely daily human activities and early diagnosis is a critical requirement for prevention and cure. Here, we induced TBI formation in the Zebra fish, a model organism, by both mechanical (ultrasonic cleaner generated convulsive shock, UGCS) and chemical (pentylenetetrazol, PTZ) methods. The TBI induced cellular and neuronal changes are monitored by measuring the activities of the indicator biomarkers viz., superoxide anion (O<sub>2</sub><sup><img>−</sup>) and glutamate by electrochemical techniques. For this, the α-lipoic acid (α-LA, LA) functionalized gold-silver (LA-Au/Ag) is used as an electrochemical sensor to diagnose the presence of these markers in physiological phosphate buffer saline (PBS, <em>pH</em> 7.4), 0.1 M KCl solutions and in TBI tissues. While the oxidation of glutamate is observed in the potential window 0.2–0.5 V, the metal mediated oxidation of O<sub>2</sub><sup><img>−</sup> is observed at the potential window 0.6–1.0 V. The sensor showed good linear ranges for O<sub>2</sub><sup><img>−</sup> (from 4 to 48 μM with the LOD of 4 μM for the O<sub>2</sub><sup><img>−</sup> detection) and glutamate (from 20 to 130 μM with the LOD 19 μM). The TBI tissue modified electrode showed lower resistance than the normal brain tissue ((NBT), as control) due to the presence of higher amount of O<sub>2</sub><sup><img>−</sup> and occurrence of Fenton’s and Heber-Weise’s reactions in the presence of [Fe(CN)<sub>6</sub>]<sup>3−/4−</sup>. For theragnostic application, the LA-Au/Ag nanoparticles is delivered into the UGCS and PTZ treated Zebrafish and electrochemical signal changes are monitored by cyclic voltammetry and impedance spectroscopy. Electrochemical data further corroborated with the activities of superoxide dismutase (SOD), Catalase (CAT) and lipid peroxidase (MDA) in parallel. The developed method of electrochemical sensing of TBI may provide alternative for the early TBI diagnosis and therapeutics for the prevention of TBI.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"163 ","pages":"Article 108871"},"PeriodicalIF":4.8,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142790743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-02DOI: 10.1016/j.bioelechem.2024.108863
Ulviye Kilimci , Baha Öndeş , Çağdaş Sunna , Murat Uygun , Deniz Aktaş Uygun
In this study, gold nanoparticles (AuNPs) were synthesized and combined with fullerene, resulting in the formation of nanocomposite structures. The structures were then characterized by scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDX) techniques. The nanostructures were functionalized with MPA and employed for covalent binding of CA125 antibody, whereby the antibody-bound nanocomposite structure was utilized for modification of the surface of the SPE. The surface of the immunosensor was protected by Nafion, and the individual stages of the immunosensor design were characterized by CV and EIS. CA125 determination was conducted using the EIS technique, which revealed a linear concentration range of 1–100 U·mL−1 and a LOD value of 0.016 U·mL−1. The immunosensor demonstrated selective recognition of CEA, NSE, HSA, and IgG proteins, exhibiting good reproducibility. The prepared immunosensor demonstrated 80.9% activity even after a 30-day period. Moreover, this immunosensor can be successfully employed in conventional clinical human serum applications. A comparison with existing literature reveals that the superior features of this immunosensor are its low LOD and high stability. Additionally, the short analysis time in comparison to commercial kits is considered a significant advantage. The prepared immunosensor displays valuable characteristics for the determination of CA125, and it has the potential to be developed for use in health applications.
{"title":"Development of label-free immunosensors based on AuNPs-fullerene nanocomposites for the determination of cancer antigen 125","authors":"Ulviye Kilimci , Baha Öndeş , Çağdaş Sunna , Murat Uygun , Deniz Aktaş Uygun","doi":"10.1016/j.bioelechem.2024.108863","DOIUrl":"10.1016/j.bioelechem.2024.108863","url":null,"abstract":"<div><div>In this study, gold nanoparticles (AuNPs) were synthesized and combined with fullerene, resulting in the formation of nanocomposite structures. The structures were then characterized by scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDX) techniques. The nanostructures were functionalized with MPA and employed for covalent binding of CA125 antibody, whereby the antibody-bound nanocomposite structure was utilized for modification of the surface of the SPE. The surface of the immunosensor was protected by Nafion, and the individual stages of the immunosensor design were characterized by CV and EIS. CA125 determination was conducted using the EIS technique, which revealed a linear concentration range of 1–100 U·mL<sup>−1</sup> and a LOD value of 0.016 U·mL<sup>−1</sup>. The immunosensor demonstrated selective recognition of CEA, NSE, HSA, and IgG proteins, exhibiting good reproducibility. The prepared immunosensor demonstrated 80.9% activity even after a 30-day period. Moreover, this immunosensor can be successfully employed in conventional clinical human serum applications. A comparison with existing literature reveals that the superior features of this immunosensor are its low LOD and high stability. Additionally, the short analysis time in comparison to commercial kits is considered a significant advantage. The prepared immunosensor displays valuable characteristics for the determination of CA125, and it has the potential to be developed for use in health applications.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"163 ","pages":"Article 108863"},"PeriodicalIF":4.8,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142790742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-30DOI: 10.1016/j.bioelechem.2024.108859
Mohammed Arroussi , Jiajia Wu , Liyang Zhu , Peng Wang , Dun Zhang , Wenkai Wang
Understanding selective growth of electroactive bacteria on surface of constituent phases (ferrite/ austenite) of 2205 DSS is required for mitigating microbiologically influenced corrosion (MIC). In this study, the preferential attachment of bacteria and its impact on corrosion of single phase were investigated under anaerobic condition using Shewanella oneidensis MR-1. Single-ferrite phase was more susceptible to biofilm formation compared with single-austenite phase. Atomic force microscope (AFM) revealed that the surface of ferrite phase coupon was fully covered with S. oneidensis MR-1 biofilm whereas few S. oneidensis MR-1 cells were observed on the surface of austenite phase. After 14 d of incubation, the maximum biofilm thicknesses on 2205 DSS, ferrite and austenite phase were 15.5 ± 1.0 µm, 13.8 ± 3.2 µm, and 10.2 ± 0.8 µm, respectively. S. oneidensis MR-1 accelerated the pitting corrosion of materials. The maximum pits depth on single ferrite and austenite phase in sterile medium (3.2 µm vs 2.2 µm with mean values 2.5 µm vs 1.7 µm) were relatively small than those in biotic medium (6.0 µm vs 4.5 µm with mean values 4.5 µm vs 3.8 µm). Synergistic effects of Cr and Ni enhanced the stability of passive film on austenite phase.
了解2205 DSS组成相（铁素体/奥氏体）表面电活性细菌的选择性生长是减轻微生物影响腐蚀（MIC）的必要条件。在厌氧条件下，利用希瓦氏菌MR-1研究了细菌的优先附着及其对单相腐蚀的影响。单铁素体相比单奥氏体相更容易形成生物膜。原子力显微镜（AFM）观察发现，铁素体相表面覆盖了一层完整的奥氏体链球菌MR-1生物膜，而奥氏体相表面仅观察到少量的奥氏体链球菌MR-1细胞。培养14 d后，2205 DSS、铁素体和奥氏体相的最大生物膜厚度分别为15.5±1.0µm、13.8±3.2µm和10.2±0.8µm。S. oneidensis MR-1加速了材料的点蚀。无菌培养基中单个铁素体和奥氏体相的最大凹坑深度（3.2µm vs 2.2µm，平均值为2.5µm vs 1.7µm）相对小于生物培养基（6.0µm vs 4.5µm，平均值为4.5µm vs 3.8µm）。Cr和Ni的协同作用增强了钝化膜在奥氏体相上的稳定性。
{"title":"Effect of preferential growth of Shewanella oneidensis MR-1 on microbial corrosion of constituent phases of 2205 duplex stainless steel","authors":"Mohammed Arroussi , Jiajia Wu , Liyang Zhu , Peng Wang , Dun Zhang , Wenkai Wang","doi":"10.1016/j.bioelechem.2024.108859","DOIUrl":"10.1016/j.bioelechem.2024.108859","url":null,"abstract":"<div><div>Understanding selective growth of electroactive bacteria on surface of constituent phases (ferrite/ austenite) of 2205 DSS is required for mitigating microbiologically influenced corrosion (MIC). In this study, the preferential attachment of bacteria and its impact on corrosion of single phase were investigated under anaerobic condition using <em>Shewanella oneidensis</em> MR-1. Single-ferrite phase was more susceptible to biofilm formation compared with single-austenite phase. Atomic force microscope (AFM) revealed that the surface of ferrite phase coupon was fully covered with <em>S. oneidensis</em> MR-1 biofilm whereas few <em>S. oneidensis</em> MR-1 cells were observed on the surface of austenite phase. After 14 d of incubation, the maximum biofilm thicknesses on 2205 DSS, ferrite and austenite phase were 15.5 ± 1.0 µm, 13.8 ± 3.2 µm, and 10.2 ± 0.8 µm, respectively. <em>S. oneidensis</em> MR-1 accelerated the pitting corrosion of materials. The maximum pits depth on single ferrite and austenite phase in sterile medium (3.2 µm vs 2.2 µm with mean values 2.5 µm vs 1.7 µm) were relatively small than those in biotic medium (6.0 µm vs 4.5 µm with mean values 4.5 µm vs 3.8 µm). Synergistic effects of Cr and Ni enhanced the stability of passive film on austenite phase.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"163 ","pages":"Article 108859"},"PeriodicalIF":4.8,"publicationDate":"2024-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142783623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-28DOI: 10.1016/j.bioelechem.2024.108857
Zhao Huang , Kaimin Wu , Feiye Ju , Ran He , Ying Tang , Yao Chen , Xuliang He , Jing Zhang , Libo Nie
Early cancer diagnosis is paramount for enhancing treatment efficacy, extending patient survival, and improving the quality of life. We developed a highly sensitive electrochemical biosensor for the detection of target DNA (tDNA) associated with gastric cancer. This advancement integrates dual signal amplification strategies: bio-barcode amplification (BCA) and surface-initiated enzyme polymerization (SIEP), with copper nanoclusters (CuNCs) serving as signal labels. Silica nanoparticles (SiO2) were covalently linked with polythymine (poly T) and complementary DNA to create bio-barcode probes. These probes, through hybridization, were immobilized on the reduced graphene oxide and Au nanoparticle (rGO-AuNPs) modified interface and marking the first amplification of the electrical signal. Subsequently, the extended poly T prompted by SIEP bound additional CuNCs through the combination of T-Cu2+, leading to a second round of signal amplification. The biosensor demonstrated a minimum detection limit of 0.13 fmol/L over a linear response range from 1 fmol/L to 1 nmol/L. It also showcased excellent specificity, repeatability, and stability, making it a promising tool for the sensitive detection of gastric cancer biomarkers.
{"title":"Copper nanocluster based cascade amplified DNA electrochemical detection combining with bio-barcode assay and surface-initiated enzyme polymerization","authors":"Zhao Huang , Kaimin Wu , Feiye Ju , Ran He , Ying Tang , Yao Chen , Xuliang He , Jing Zhang , Libo Nie","doi":"10.1016/j.bioelechem.2024.108857","DOIUrl":"10.1016/j.bioelechem.2024.108857","url":null,"abstract":"<div><div>Early cancer diagnosis is paramount for enhancing treatment efficacy, extending patient survival, and improving the quality of life. We developed a highly sensitive electrochemical biosensor for the detection of target DNA (tDNA) associated with gastric cancer. This advancement integrates dual signal amplification strategies: bio-barcode amplification (BCA) and surface-initiated enzyme polymerization (SIEP), with copper nanoclusters (CuNCs) serving as signal labels. Silica nanoparticles (SiO<sub>2</sub>) were covalently linked with polythymine (poly T) and complementary DNA to create bio-barcode probes. These probes, through hybridization, were immobilized on the reduced graphene oxide and Au nanoparticle (rGO-AuNPs) modified interface and marking the first amplification of the electrical signal. Subsequently, the extended poly T prompted by SIEP bound additional CuNCs through the combination of T-Cu<sup>2+</sup>, leading to a second round of signal amplification. The biosensor demonstrated a minimum detection limit of 0.13 fmol/L over a linear response range from 1 fmol/L to 1 nmol/L. It also showcased excellent specificity, repeatability, and stability, making it a promising tool for the sensitive detection of gastric cancer biomarkers.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"163 ","pages":"Article 108857"},"PeriodicalIF":4.8,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142759075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-28DOI: 10.1016/j.bioelechem.2024.108851
Mojmír Trefulka, Hana Černocká, Tatiana Staroňová, Veronika Ostatná
Recently, it was described that neutral glycans can be distinguished from those containing sialic acid at the mercury electrode after modification with osmium(VI) N,N,N',N'-tetramethylethylenediamine (Os(VI)tem). Our work shows the possibility of studying glycans and glycoproteins at pyrolytic graphite electrodes depending on the presence of sialic acid.
Short glycans, glycans released from glycoproteins, and glycoproteins themselves yielded similar voltammetric responses after their modification by Os(VI)tem. Os(VI)tem modified glycans and glycoproteins produced a couple of cathodic and anodic peaks. Changing peak heights and potentials of glycans and glycoproteins pointed out the presence of sialic acid. These findings could be utilized to improve glycoprotein sensing by chemical modification.
{"title":"Voltammetric analysis of glycoproteins containing sialylated and neutral glycans at pyrolytic graphite electrode","authors":"Mojmír Trefulka, Hana Černocká, Tatiana Staroňová, Veronika Ostatná","doi":"10.1016/j.bioelechem.2024.108851","DOIUrl":"10.1016/j.bioelechem.2024.108851","url":null,"abstract":"<div><div>Recently, it was described that neutral glycans can be distinguished from those containing sialic acid at the mercury electrode after modification with osmium(VI) <em>N</em>,<em>N</em>,<em>N</em>',<em>N</em>'-tetramethylethylenediamine (Os(VI)tem). Our work shows the possibility of studying glycans and glycoproteins at pyrolytic graphite electrodes depending on the<!--> <!-->presence of sialic acid.</div><div>Short glycans, glycans released from glycoproteins, and glycoproteins themselves yielded similar voltammetric responses after their modification by Os(VI)tem. Os(VI)tem modified glycans and glycoproteins produced a<!--> <!-->couple of cathodic and anodic peaks. Changing peak heights and potentials of glycans and glycoproteins pointed out the presence of sialic acid. These findings could be utilized to improve glycoprotein sensing by chemical modification.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"163 ","pages":"Article 108851"},"PeriodicalIF":4.8,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142783628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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