Pub Date : 2025-11-01DOI: 10.1016/j.bioelechem.2025.109158
Sadaf Shakeel , Mohammad Zain Khan , Mariya Shakeel
Development of highly stable, conductive, biocompatible and cost-effective cathode is crucial for scaling up microbial electrosynthesis (MES). Bimetallic cathodes have gain importance in recent times due wide application. Current study utilized a bimetallic (nickel and iron) impregnated granular activated carbon (Ni-Fe-GAC) cathode in microbial electrosynthesis. Ni-Fe-GAC cathode was prepared via treating GACs in a mixed solution of nickel chloride and iron sulphate. Deposition of metals on GAC facilitates the improved electron transfer and biofilm formation on the GAC surface, giving 1.4 times higher acetate production than plain GAC (control). Scanning electron microscopy (SEM) confirmed the well adaptation of anaerobic microbes on the surface of Ni-Fe-GAC cathode. Linear sweep voltammetry (LSV) confirmed the better electrochemical performance of Ni-Fe-GAC cathode. A regeneration test was also conducted via acid washing to remove metals from Ni-Fe-GAC followed by reloading. Regeneration successfully restored cathode performance, making Ni-Fe-GAC suitable for long-term application.
{"title":"Microbial electrosynthesis of acetic acid from carbon dioxide using a bimetallic-granular activated carbon cathode","authors":"Sadaf Shakeel , Mohammad Zain Khan , Mariya Shakeel","doi":"10.1016/j.bioelechem.2025.109158","DOIUrl":"10.1016/j.bioelechem.2025.109158","url":null,"abstract":"<div><div>Development of highly stable, conductive, biocompatible and cost-effective cathode is crucial for scaling up microbial electrosynthesis (MES). Bimetallic cathodes have gain importance in recent times due wide application. Current study utilized a bimetallic (nickel and iron) impregnated granular activated carbon (Ni-Fe-GAC) cathode in microbial electrosynthesis. Ni-Fe-GAC cathode was prepared via treating GACs in a mixed solution of nickel chloride and iron sulphate. Deposition of metals on GAC facilitates the improved electron transfer and biofilm formation on the GAC surface, giving 1.4 times higher acetate production than plain GAC (control). Scanning electron microscopy (SEM) confirmed the well adaptation of anaerobic microbes on the surface of Ni-Fe-GAC cathode. Linear sweep voltammetry (LSV) confirmed the better electrochemical performance of Ni-Fe-GAC cathode. A regeneration test was also conducted via acid washing to remove metals from Ni-Fe-GAC followed by reloading. Regeneration successfully restored cathode performance, making Ni-Fe-GAC suitable for long-term application.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"168 ","pages":"Article 109158"},"PeriodicalIF":4.5,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145463040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-10-30DOI: 10.1016/j.bioelechem.2025.109156
Dmitry Yu. Amsheev , Alexei V. Kashevskii , Igor A. Ponomarenko , Alexander Yu. Safronov , Vladlen V. Akimov , Vladimir L. Tauson , Olga Yu. Belozerova , Igor V. Klimenkov , Irina A. Shurygina , Natalya N. Dremina , Irina S. Trukhan , Evgeniya A. Lozovskaya , Alena S. Zavyalova , Sergey B. Nikiforov
An anodic polarization of the commercially pure titanium samples in 0.1 M phosphate buffer solution (pH 7.4) in the range of potentials 0.00 to 1.00 V provides an electrochemical surface modification due to controlled passivation. The titanium oxide film thickness calculated using electrochemical impedance spectroscopy falls in two ranges: 8–11 nm for experiments conducted at open circuit potentials, and 12–26 nm for experiments under conditions of anodic polarization. The maximum value of the root mean square roughness (Ra = 80 nm) was obtained using a polarization potential of 0.75 V. At the same potential, minimal interaction of rat's fibroblasts with the titanium samples surface was observed. Thus, the analysis methods used in the work made it possible to quantify the inverse relationship between the surface heterogeneity and the adhesion of model cells in vitro.
{"title":"Morphology and biological characteristics of the electrochemically modified titanium surface","authors":"Dmitry Yu. Amsheev , Alexei V. Kashevskii , Igor A. Ponomarenko , Alexander Yu. Safronov , Vladlen V. Akimov , Vladimir L. Tauson , Olga Yu. Belozerova , Igor V. Klimenkov , Irina A. Shurygina , Natalya N. Dremina , Irina S. Trukhan , Evgeniya A. Lozovskaya , Alena S. Zavyalova , Sergey B. Nikiforov","doi":"10.1016/j.bioelechem.2025.109156","DOIUrl":"10.1016/j.bioelechem.2025.109156","url":null,"abstract":"<div><div>An anodic polarization of the commercially pure titanium samples in 0.1 M phosphate buffer solution (pH 7.4) in the range of potentials 0.00 to 1.00 V provides an electrochemical surface modification due to controlled passivation. The titanium oxide film thickness calculated using electrochemical impedance spectroscopy falls in two ranges: 8–11 nm for experiments conducted at open circuit potentials, and 12–26 nm for experiments under conditions of anodic polarization. The maximum value of the root mean square roughness (R<sub>a</sub> = 80 nm) was obtained using a polarization potential of 0.75 V. At the same potential, minimal interaction of rat's fibroblasts with the titanium samples surface was observed. Thus, the analysis methods used in the work made it possible to quantify the inverse relationship between the surface heterogeneity and the adhesion of model cells <em>in vitro</em>.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"168 ","pages":"Article 109156"},"PeriodicalIF":4.5,"publicationDate":"2025-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145457036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-10-29DOI: 10.1016/j.bioelechem.2025.109155
Olha Demkiv , Nataliya Stasyuk , Marcin Holdynski , Wojciech Nogala , Mykhailo Gonchar
A novel bioelectrochemical sensor has been developed for the selective detection of homovanillic acid (HVA), an important urinary biomarker of neuroblastoma. The sensor integrates green-synthesized bimetallic gold–manganese nanoparticles (nAuMnOx NPs) with laccase-like catalytic activity into a molecularly imprinted polymer (MIP) layer on a graphite electrode (MIP/nAuMnOx/GE). The nanozyme was synthesized using gallic acid as a biocompatible reducing and stabilizing agent. The MIP was formed from (3-aminopropyl)triethoxysilane as a functional monomer providing amine groups for HVA binding, tetramethyl orthosilicate as a cross-linker forming a stable silica network, and β-cyclodextrin as a supramolecular host enabling selective inclusion of the aromatic part of HVA. This composition imparted excellent molecular recognition and electrocatalytic activity. The MIP/nAuMnOx/GE sensor showed a high response at −200 mV with a limit of detection 0.12 μM and a linear range of 1–30 μM. Sensitivity reached up to 1.39 × 104 A·M−1·m−2, representing a 14-fold enhancement over natural laccase-based sensors. The sensor demonstrated outstanding selectivity toward HVA against dopamine, 5-hydroxyindoleacetic acid, vanillylmandelic acid, uric, and ascorbic acids, and enabled direct HVA quantification in untreated human urine with recovery rates of 97.5–102 %. The synergistic integration of nAuMnOx nanozyme catalysis and MIP recognition establishes a robust platform for noninvasive neuroblastoma diagnostics.
{"title":"Laccase-mimicking nAuMnOx integrated into a molecularly imprinted electrochemical sensor for selective homovanillic acid detection in human urine","authors":"Olha Demkiv , Nataliya Stasyuk , Marcin Holdynski , Wojciech Nogala , Mykhailo Gonchar","doi":"10.1016/j.bioelechem.2025.109155","DOIUrl":"10.1016/j.bioelechem.2025.109155","url":null,"abstract":"<div><div>A novel bioelectrochemical sensor has been developed for the selective detection of homovanillic acid (HVA), an important urinary biomarker of neuroblastoma. The sensor integrates green-synthesized bimetallic gold–manganese nanoparticles (nAuMnO<sub>x</sub> NPs) with laccase-like catalytic activity into a molecularly imprinted polymer (MIP) layer on a graphite electrode (MIP/nAuMnO<sub>x</sub>/GE). The nanozyme was synthesized using gallic acid as a biocompatible reducing and stabilizing agent. The MIP was formed from (3-aminopropyl)triethoxysilane as a functional monomer providing amine groups for HVA binding, tetramethyl orthosilicate as a cross-linker forming a stable silica network, and β-cyclodextrin as a supramolecular host enabling selective inclusion of the aromatic part of HVA. This composition imparted excellent molecular recognition and electrocatalytic activity. The MIP/nAuMnO<sub>x</sub>/GE sensor showed a high response at −200 mV with a limit of detection 0.12 μM and a linear range of 1–30 μM. Sensitivity reached up to 1.39 × 10<sup>4</sup> A·M<sup>−1</sup>·m<sup>−2</sup>, representing a 14-fold enhancement over natural laccase-based sensors. The sensor demonstrated outstanding selectivity toward HVA against dopamine, 5-hydroxyindoleacetic acid, vanillylmandelic acid, uric, and ascorbic acids, and enabled direct HVA quantification in untreated human urine with recovery rates of 97.5–102 %. The synergistic integration of nAuMnO<sub>x</sub> nanozyme catalysis and MIP recognition establishes a robust platform for noninvasive neuroblastoma diagnostics.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"168 ","pages":"Article 109155"},"PeriodicalIF":4.5,"publicationDate":"2025-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145457087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-10-28DOI: 10.1016/j.bioelechem.2025.109152
Julia Czopinska , Filip Budny , Andrzej Peplowski , Anna Sobiepanek , Marta Jarczewska
Carbon – based surfaces are becoming attractive alternative to gold transducers, which are commonly applied for development of nucleic acid sensors. However, one of the challenges is the necessity of elaboration of carbon transducers modification methods, yielding stable receptor layers that are capable of binding to target analytes. Herein, we present the studies on the elaboration of aptamer layers aimed for electrochemical detection of cytokine - tumor necrosis factor-alpha (TNF-α). This protein can serve as biomarker of wound healing processes that might be the answer to need for rapid determination of the stage of the wound that can result in selection of proper diagnosis as well as treatment strategy. We aimed on functionalization of edge – plane pyrolytic graphite as well as planar screen – printed electrodes. The aptamer layers were formed through one-step procedure using pyrene and anthracene anchor groups conjugated with aptamers. The research enabled to choose ferri/ferrocyanide redox indicator that was the source of electrochemical signal, determine the range of linear response (5–200 ng/L) with LOD of 5.07 ng/L and test the sensor against complex samples of serum or simulated wound exudate. The developed aptasensor proved to be promising solution towards non-invasive monitoring of wound healing.
{"title":"Application of aptamer – carbon hybrid materials for electrochemical detection of wound healing biomarker – TNF-α protein","authors":"Julia Czopinska , Filip Budny , Andrzej Peplowski , Anna Sobiepanek , Marta Jarczewska","doi":"10.1016/j.bioelechem.2025.109152","DOIUrl":"10.1016/j.bioelechem.2025.109152","url":null,"abstract":"<div><div>Carbon – based surfaces are becoming attractive alternative to gold transducers, which are commonly applied for development of nucleic acid sensors. However, one of the challenges is the necessity of elaboration of carbon transducers modification methods, yielding stable receptor layers that are capable of binding to target analytes. Herein, we present the studies on the elaboration of aptamer layers aimed for electrochemical detection of cytokine - tumor necrosis factor-alpha (TNF-α). This protein can serve as biomarker of wound healing processes that might be the answer to need for rapid determination of the stage of the wound that can result in selection of proper diagnosis as well as treatment strategy. We aimed on functionalization of edge – plane pyrolytic graphite as well as planar screen – printed electrodes. The aptamer layers were formed through one-step procedure using pyrene and anthracene anchor groups conjugated with aptamers. The research enabled to choose ferri/ferrocyanide redox indicator that was the source of electrochemical signal, determine the range of linear response (5–200 ng/L) with LOD of 5.07 ng/L and test the sensor against complex samples of serum or simulated wound exudate. The developed aptasensor proved to be promising solution towards non-invasive monitoring of wound healing.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"168 ","pages":"Article 109152"},"PeriodicalIF":4.5,"publicationDate":"2025-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145412996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-10-28DOI: 10.1016/j.bioelechem.2025.109147
Yemin Han , Haotian Yu , Manman Lv , Han Gao , Weiming Lin , Weihao Li , Mengqing Cheng , Yan Huang , Dianhuai Meng , Tian Wen , Zuhong Lu , Quanjun Liu
Bacterial infections are highly prevalent globally, and the health issues they induce often lead to numerous severe problems for human well-being, demanding timely and accurate detection strategies. Herein, We developed a universal electrochemical biosensor for pathogen screening, offering high sensitivity, specificity, rapidity, and multiplex detection. The platform integrates interdigitated electrodes for low-voltage pathogen lysis and nucleic acid release with asymmetric recombinase polymerase amplification (aRPA) to produce single-stranded DNA, simplifying extraction and reducing detection time. Screen-printed electrodes were carboxylated using diazonium salts to immobilize Fc-labeled hairpin DNA via amide bonds. Upon applying a positive voltage, amplified DNA hybridizes with the hairpin probes, distancing Fc molecules from the electrode surface and diminishing electrochemical signals, effectively eliminating false positives. Optimized conditions enabled detection sensitivities of 10 CFU/mL for Staphylococcus aureus and 5 CFU/mL for Acinetobacter baumannii. Additionally, Spiked testing in tap water, milk, and lake water demonstrated consistency with plate counting, validating the rapid system's accuracy and applicability. Remarkably, the assay time was reduced from 6 to 8 h to 25 min while maintaining pathogen specificity. This biosensor shows promise for foodborne pathogen surveillance, environmental monitoring, and point-of-care diagnostics, offering a streamlined platform for rapid, accurate pathogen identification.
{"title":"Dual-mode electro-driven biosensor: Low-voltage lysis and hybridization synergy for rapid and sensitive pathogen screening","authors":"Yemin Han , Haotian Yu , Manman Lv , Han Gao , Weiming Lin , Weihao Li , Mengqing Cheng , Yan Huang , Dianhuai Meng , Tian Wen , Zuhong Lu , Quanjun Liu","doi":"10.1016/j.bioelechem.2025.109147","DOIUrl":"10.1016/j.bioelechem.2025.109147","url":null,"abstract":"<div><div>Bacterial infections are highly prevalent globally, and the health issues they induce often lead to numerous severe problems for human well-being, demanding timely and accurate detection strategies. Herein, We developed a universal electrochemical biosensor for pathogen screening, offering high sensitivity, specificity, rapidity, and multiplex detection. The platform integrates interdigitated electrodes for low-voltage pathogen lysis and nucleic acid release with asymmetric recombinase polymerase amplification (aRPA) to produce single-stranded DNA, simplifying extraction and reducing detection time. Screen-printed electrodes were carboxylated using diazonium salts to immobilize Fc-labeled hairpin DNA via amide bonds. Upon applying a positive voltage, amplified DNA hybridizes with the hairpin probes, distancing Fc molecules from the electrode surface and diminishing electrochemical signals, effectively eliminating false positives. Optimized conditions enabled detection sensitivities of 10 CFU/mL for <em>Staphylococcus aureus</em> and 5 CFU/mL for <em>Acinetobacter baumannii</em>. Additionally, Spiked testing in tap water, milk, and lake water demonstrated consistency with plate counting, validating the rapid system's accuracy and applicability. Remarkably, the assay time was reduced from 6 to 8 h to 25 min while maintaining pathogen specificity. This biosensor shows promise for foodborne pathogen surveillance, environmental monitoring, and point-of-care diagnostics, offering a streamlined platform for rapid, accurate pathogen identification.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"168 ","pages":"Article 109147"},"PeriodicalIF":4.5,"publicationDate":"2025-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145412997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The twin microbial fuel cell powered electro-Fenton system (twin-MFCⓅEFs), combining active oxygen component and microbial metabolism, was constructed to improve the treatment process of waste activated sludge (WAS). Nevertheless, the performance and mechanism of electron transfer underlying this enhancement remain poorly understood. This study investigated the performance and mechanism of electron generation and utilization in twin-MFCⓅEFs with WAS as substrate. The higher electron generation and recovery efficiency (8.25 % of coulombic efficiency) was attributed to the higher content of amino acids (such as tryptophan), humic substances and their aromatic groups and unsaturated conjugated double bonds in the soluble organic matter, which facilitated biodegradation and electron transfer. The higher electron utilization performance (52.76 % of faraday efficiency) relied on the superior electron supply system that exhibited greater free radical oxidation. Metagenomic analysis indicated that an increased secretory capacity of glycosyltransferases (including glucosyltransferases and β-glucosidases) and a reduced activity of acetate kinase and methyl-coenzyme M reductase alpha subunit in cellular metabolic processes favored signaling and electricity production. The study focused on electron flow in twin-MFCⓅEFs and offered a promising strategy for improving the sludge treatment process.
{"title":"Electron transfer performance and mechanism in twin microbial fuel cell powered electro-Fenton system with waste activated sludge as substrate","authors":"Jiaqi Lv , Qingliang Zhao , Junqiu Jiang , Jing Ding , Liangliang Wei , Jiawen Liang","doi":"10.1016/j.bioelechem.2025.109154","DOIUrl":"10.1016/j.bioelechem.2025.109154","url":null,"abstract":"<div><div>The twin microbial fuel cell powered electro-Fenton system (twin-MFCⓅEFs), combining active oxygen component and microbial metabolism, was constructed to improve the treatment process of waste activated sludge (WAS). Nevertheless, the performance and mechanism of electron transfer underlying this enhancement remain poorly understood. This study investigated the performance and mechanism of electron generation and utilization in twin-MFCⓅEFs with WAS as substrate. The higher electron generation and recovery efficiency (8.25 % of coulombic efficiency) was attributed to the higher content of amino acids (such as tryptophan), humic substances and their aromatic groups and unsaturated conjugated double bonds in the soluble organic matter, which facilitated biodegradation and electron transfer. The higher electron utilization performance (52.76 % of faraday efficiency) relied on the superior electron supply system that exhibited greater free radical oxidation. Metagenomic analysis indicated that an increased secretory capacity of glycosyltransferases (including glucosyltransferases and β-glucosidases) and a reduced activity of acetate kinase and methyl-coenzyme M reductase alpha subunit in cellular metabolic processes favored signaling and electricity production. The study focused on electron flow in twin-MFCⓅEFs and offered a promising strategy for improving the sludge treatment process.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"168 ","pages":"Article 109154"},"PeriodicalIF":4.5,"publicationDate":"2025-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145385494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The treatment of lipid-rich wastewater using microbial fuel cells (MFCs) is often limited by the low solubility and bioavailability of hydrophobic substrates such as fat, oil, and grease (FOG). In this study, we present a sustainable and circular strategy wherein biosurfactants (BSFs) are produced from FOG using Bacillus velezensis and used to enhance FOG bioavailability and consequent degradation in MFCs. BSF production (2.3 g/L) was confirmed via foaming, drop collapse, oil displacement, CTAB-methylene blue agar, and emulsification index assays. When used to increase the bioavailability of FOG, the presence of BSFs improved all key MFC performance metrics. Notably, a 5.5-fold increase in maximum power density was observed from 0.08 to 0.44 W/m2 when BSFs were added and FOG was used as the sole organic substrate at a 0.27% (v/v) concentration. Current density (1.1 A/m2), COD removal (81.1%), and coulombic efficiency (7.9%) also improved when BSFs were present. The control MFCs operated without BSF showed significant performance deterioration, attributed to poor emulsification, substrate accumulation, and limited availability of substrate. Comparative tests using triolein as a model lipidic substrate highlighted the effectiveness of BSF-assisted FOG degradation.
{"title":"A circular valorization of fat, oil, grease (FOG): Integrating FOG-derived biosurfactant production and FOG-fed microbial fuel cells","authors":"Min-Gu Sim , Ruggero Rossi , Geon-Soo Ha , Gahyun Baek","doi":"10.1016/j.bioelechem.2025.109151","DOIUrl":"10.1016/j.bioelechem.2025.109151","url":null,"abstract":"<div><div>The treatment of lipid-rich wastewater using microbial fuel cells (MFCs) is often limited by the low solubility and bioavailability of hydrophobic substrates such as fat, oil, and grease (FOG). In this study, we present a sustainable and circular strategy wherein biosurfactants (BSFs) are produced from FOG using <em>Bacillus velezensis</em> and used to enhance FOG bioavailability and consequent degradation in MFCs. BSF production (2.3 g/L) was confirmed via foaming, drop collapse, oil displacement, CTAB-methylene blue agar, and emulsification index assays. When used to increase the bioavailability of FOG, the presence of BSFs improved all key MFC performance metrics. Notably, a 5.5-fold increase in maximum power density was observed from 0.08 to 0.44 W/m<sup>2</sup> when BSFs were added and FOG was used as the sole organic substrate at a 0.27% (v/v) concentration. Current density (1.1 A/m<sup>2</sup>), COD removal (81.1%), and coulombic efficiency (7.9%) also improved when BSFs were present. The control MFCs operated without BSF showed significant performance deterioration, attributed to poor emulsification, substrate accumulation, and limited availability of substrate. Comparative tests using triolein as a model lipidic substrate highlighted the effectiveness of BSF-assisted FOG degradation.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"168 ","pages":"Article 109151"},"PeriodicalIF":4.5,"publicationDate":"2025-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145407769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-10-24DOI: 10.1016/j.bioelechem.2025.109153
Praveen Sahu , Ignacio G. Camarillo , Raji Sundararajan
High-throughput, label-free quantitative proteomic analyses, along with cell viability and reactive oxygen species (ROS) studies, were performed on MDA-MB-468 human triple-negative breast cancer (TNBC) cells, to gain mechanistic insights into therapeutic actions. TNBC was chosen as it is the most lethal subtype of breast cancer, highly aggressive, prone to recurrence and metastasis, with the highest prevalence in black women. The absence of specific biomarkers limits targeted therapies. This unmet need was studied using proteomics, after treating the cells with electrical pulses (EP) combined with metformin (met). With the EP + met treatment (1000 V/cm, 1 mM), cell viability dropped to 25.6 % after 24 h and ROS increased to 179 %, compared to control at 100 %. Proteomics revealed 125 upregulated and 37 downregulated proteins in EP + met, compared to met alone, involving enzymes, proliferation markers, and kinases. Key gene changes included upregulation of ALAD, MKI67, and LRCH4, and downregulation of EIF1AX, NSUN5, and GNS. LRCH4 and GNS are suggested to be potential novel therapeutic targets, as LRCH4 upregulation links to inhibition of the mTOR/PI3K/Akt pathway, reducing proliferation, while GNS downregulation suppresses tumor growth and metastasis. Overall, proteomics-based preliminary findings suggest that EP + met modulate TNBC pathways, identifying potential biomarkers and providing a foundation for future validation
{"title":"Investigations of potential therapeutic targets from high-throughput, label-free, quantitative proteomic studies in MDA-MB-468 cells treated with metformin and electrical pulses","authors":"Praveen Sahu , Ignacio G. Camarillo , Raji Sundararajan","doi":"10.1016/j.bioelechem.2025.109153","DOIUrl":"10.1016/j.bioelechem.2025.109153","url":null,"abstract":"<div><div>High-throughput, label-free quantitative proteomic analyses, along with cell viability and reactive oxygen species (ROS) studies, were performed on MDA-MB-468 human triple-negative breast cancer (TNBC) cells, to gain mechanistic insights into therapeutic actions. TNBC was chosen as it is the most lethal subtype of breast cancer, highly aggressive, prone to recurrence and metastasis, with the highest prevalence in black women. The absence of specific biomarkers limits targeted therapies. This unmet need was studied using proteomics, after treating the cells with electrical pulses (EP) combined with metformin (met). With the EP + met treatment (1000 V/cm, 1 mM), cell viability dropped to 25.6 % after 24 h and ROS increased to 179 %, compared to control at 100 %. Proteomics revealed 125 upregulated and 37 downregulated proteins in EP + met, compared to met alone, involving enzymes, proliferation markers, and kinases. Key gene changes included upregulation of ALAD, MKI67, and LRCH4, and downregulation of EIF1AX, NSUN5, and GNS. LRCH4 and GNS are suggested to be potential novel therapeutic targets, as LRCH4 upregulation links to inhibition of the mTOR/PI3K/Akt pathway, reducing proliferation, while GNS downregulation suppresses tumor growth and metastasis. Overall, proteomics-based preliminary findings suggest that EP + met modulate TNBC pathways, identifying potential biomarkers and providing a foundation for future validation</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"168 ","pages":"Article 109153"},"PeriodicalIF":4.5,"publicationDate":"2025-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145511377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-10-24DOI: 10.1016/j.bioelechem.2025.109149
David Hernández-Villamor , Aya Jeaidi , Riet Boydens , Korneel Rabaey , Tom Van de Wiele , Antonin Prévoteau
An increasing number of microbial species within the human body, many of which are pathogenic, are being reported as “electroactive”. However, the mechanisms and kinetics of extracellular electron transfer (EET) and its putative ecological relevance remain understudied. We utilized rotating disk electrodes (RDEs) to assess mediated electron transfer (MET) in five oral species via their ability to reduce riboflavin and ferricyanide. The use of both mediators was confirmed in Streptococcus mutans, Fusobacterium nucleatum, Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis, while A. viscosus only reduced riboflavin. Kinetics of EET (turnover rate per cell) were slow with riboflavin (kcat, RF 104 s−1) in all species but F. nucleatum, whereas ferricyanide resulted in fast kinetics (kcat, Ferri(app) 104 s−1) in all but S. mutans. Due to its central role in oral biofilms and association to systemic diseases, MET was further characterized in F. nucleatum. Apparent Michaelis–Menten kinetics showed Km values of (0.57 ± 0.16 and 10.43 ± 0.91) M for ferricyanide and riboflavin. The presence of mediators enhanced acetate production compared to mediator-free controls; when ferricyanide was used, butyrate and formate production was triggered only after its depletion. Finally, the putative molecular mechanisms enabling MET in F. nucleatum are discussed.
{"title":"Mediated electron transfer in five prevalent human oral microbial species","authors":"David Hernández-Villamor , Aya Jeaidi , Riet Boydens , Korneel Rabaey , Tom Van de Wiele , Antonin Prévoteau","doi":"10.1016/j.bioelechem.2025.109149","DOIUrl":"10.1016/j.bioelechem.2025.109149","url":null,"abstract":"<div><div>An increasing number of microbial species within the human body, many of which are pathogenic, are being reported as “electroactive”. However, the mechanisms and kinetics of extracellular electron transfer (EET) and its putative ecological relevance remain understudied. We utilized rotating disk electrodes (RDEs) to assess mediated electron transfer (MET) in five oral species via their ability to reduce riboflavin and ferricyanide. The use of both mediators was confirmed in <em>Streptococcus mutans</em>, <em>Fusobacterium nucleatum</em>, <em>Aggregatibacter actinomycetemcomitans</em> and <em>Porphyromonas gingivalis</em>, while <em>A. viscosus</em> only reduced riboflavin. Kinetics of EET (turnover rate per cell) were slow with riboflavin (<em>k<sub>cat, RF</sub></em> <span><math><mo><</mo></math></span> 10<sup>4</sup> s<sup>−1</sup>) in all species but <em>F. nucleatum</em>, whereas ferricyanide resulted in fast kinetics (<em>k<sub>cat, Ferri(app)</sub></em> <span><math><mo>></mo></math></span> 10<sup>4</sup> s<sup>−1</sup>) in all but <em>S. mutans</em>. Due to its central role in oral biofilms and association to systemic diseases, MET was further characterized in <em>F. nucleatum</em>. Apparent Michaelis–Menten kinetics showed <em>K<sub>m</sub></em> values of (0.57 ± 0.16 and 10.43 ± 0.91) <span><math><mi>μ</mi></math></span>M for ferricyanide and riboflavin. The presence of mediators enhanced acetate production compared to mediator-free controls; when ferricyanide was used, butyrate and formate production was triggered only after its depletion. Finally, the putative molecular mechanisms enabling MET in <em>F. nucleatum</em> are discussed.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"168 ","pages":"Article 109149"},"PeriodicalIF":4.5,"publicationDate":"2025-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145385454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-10-22DOI: 10.1016/j.bioelechem.2025.109150
Roberta C. Martins , Pedro A. da Silva Pereira , Alexandre L.B. Baccaro , Lúcio Angnes , Adelir A. Saczk , Fabiana S. Felix
Peroxidases are oxidoreductase enzymes with intense catalytic activity toward the peroxidation of hydrogen donors in the presence of hydrogen peroxide. These enzymes are appealing for application as the biomolecular recognition step of electrochemical biosensors, since they can be easily extracted from vegetable tissues such as Solanum aethiopicum (popularly known as “Gilo”), considerably reducing the costs of sensing devices. Meanwhile, zeolites are hydrated aluminosilicates arranged in 3D tetrahedral crystalline reticules that form microporous inner structures with high internal and external surface areas, very convenient for the immobilization of high amounts of enzymes to possibly improve the electrochemical biosensors sensitivity. In this study, a peroxidase biosensor utilizing enzymes extracted from raw Gilo tissue was developed. The high adsorption capacity of zeolites (MFI-based NaY) was explored to immobilize the peroxidase enzymes. The extraction efficiency was evaluated using three different types of polymeric protectors: polyvinylpyrrolidone (PVP K90), latex, or polyvinyl alcohol (PVOH. Among them, PVOH was identified as the most effective protector. The peroxidase enzyme was immobilized on NaY zeolite, forming the peroxidase-modified zeolite that was added to carbon paste electrodes to assemble the Prx-Zeo/CPE bioelectrode. The effects of graphite powder, zeolite, mineral oil, and peroxidase concentration in the paste composition were investigated using factorial planning with the premises of the Design of Experiments (DOE) to optimize cathodic current values for hydroquinone detection. Finally, the Prx-Zeo/CPE biosensor was applied to determine the hydroquinone content in two different of skin cream samples. Hydroquinone were determined by direct interpolation of the sample analytical signals onto an analytical curve (0.2–1.0 mmol L−1) and subsequently compared with the label nominal values provided by the manufacturers, as well as with those obtained through parallel analysis using an HPLC reference method.
{"title":"Development of peroxidase-modified zeolite carbon paste electrodes (Prx-Zeo/CPE) for the biosensing of hydroquinone in pharmaceutical skin cream","authors":"Roberta C. Martins , Pedro A. da Silva Pereira , Alexandre L.B. Baccaro , Lúcio Angnes , Adelir A. Saczk , Fabiana S. Felix","doi":"10.1016/j.bioelechem.2025.109150","DOIUrl":"10.1016/j.bioelechem.2025.109150","url":null,"abstract":"<div><div>Peroxidases are oxidoreductase enzymes with intense catalytic activity toward the peroxidation of hydrogen donors in the presence of hydrogen peroxide. These enzymes are appealing for application as the biomolecular recognition step of electrochemical biosensors, since they can be easily extracted from vegetable tissues such as <em>Solanum aethiopicum</em> (popularly known as “Gilo”), considerably reducing the costs of sensing devices. Meanwhile, zeolites are hydrated aluminosilicates arranged in 3D tetrahedral crystalline reticules that form microporous inner structures with high internal and external surface areas, very convenient for the immobilization of high amounts of enzymes to possibly improve the electrochemical biosensors sensitivity. In this study, a peroxidase biosensor utilizing enzymes extracted from raw Gilo tissue was developed. The high adsorption capacity of zeolites (MFI-based NaY) was explored to immobilize the peroxidase enzymes. The extraction efficiency was evaluated using three different types of polymeric protectors: polyvinylpyrrolidone (PVP K90), latex, or polyvinyl alcohol (PVOH. Among them, PVOH was identified as the most effective protector. The peroxidase enzyme was immobilized on NaY zeolite, forming the peroxidase-modified zeolite that was added to carbon paste electrodes to assemble the <em>Prx</em>-Zeo/CPE bioelectrode. The effects of graphite powder, zeolite, mineral oil, and peroxidase concentration in the paste composition were investigated using factorial planning with the premises of the Design of Experiments (DOE) to optimize cathodic current values for hydroquinone detection. Finally, the <em>Prx</em>-Zeo/CPE biosensor was applied to determine the hydroquinone content in two different of skin cream samples. Hydroquinone were determined by direct interpolation of the sample analytical signals onto an analytical curve (0.2–1.0 mmol L<sup>−1</sup>) and subsequently compared with the label nominal values provided by the manufacturers, as well as with those obtained through parallel analysis using an HPLC reference method.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"168 ","pages":"Article 109150"},"PeriodicalIF":4.5,"publicationDate":"2025-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145385542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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