Bioelectrochemistry最新文献_第9页

High sensitivity perovskite-tagged nanobody electrochemiluminescent immunosensor for spike (S1) protein biomarker-based persistent viral reservoir detection 高灵敏度钙钛矿标记纳米体电化学发光免疫传感器，用于基于刺突（S1）蛋白生物标志物的持久性病毒库检测。

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2026-03-01 Epub Date: 2025-10-12 DOI: 10.1016/j.bioelechem.2025.109137

Jaymi January , Olivier Zwaenepoel , Nelia Sanga , Jan Gettemans , Emmanuel Iwuoha

Persistent viral reservoirs (PVR) of SARS-CoV-2 (or long COVID-19) and latent COVID-19 disease have been of great concern to clinicians. Several studies have identified spike protein (S1) as a definitive biomarker for the early detection of persistent viral reservoirs and latent COVID-19. A novel sandwich-format electrochemical immunosensor integrating a nanocomposite material was engineered for rapid and sensitive latent COVID-19 detection. The platform structure, AuSPE||strep|Nb₁|BSA|biotin|S1|strep-LiSmZrO₃-Nb₂ + BSA (AuSPE = gold screen-printed electrode, strep = streptavidin-thiol, Nb₁ = primary nanobody, Nb₂ = secondary nanobody, BSA = bovine serum albumin, and spike (S1) protein = S1), featured a disposable AuSPE modified with strep to anchor a biotinylated camelid Nb₁ specific to the spike protein. A Nb₂ conjugated to streptavidin-labelled LiSmZrO₃ perovskite completed the sandwich complex, enhancing both affinity and signal transduction. Electrochemical responses of the sensor were studied via electrochemiluminescent (ECL) signal transduction. The S1-sensitive sandwich immunosensor had a detection range of 0–1000 pg mL⁻¹ with a limit of detection of 0.04 pg mL⁻¹ via ECL. As feasibility studies, commercial spike protein in buffered solutions and human serum, highlighting the potential for the immunosensor to be used in SARS-CoV-2 patients and PVRs. The nanobody sandwich immunosensor showed excellent stability, selectivity, sensitivity, and reproducibility. The immunosensor serves as a broad PVR for a SARS-CoV-2 screening tool, detecting elevated S1 levels to enable early, targeted diagnostics.

SARS-CoV-2（或长型COVID-19）和潜伏性COVID-19疾病的持续病毒库（PVR）一直是临床医生关注的焦点。一些研究已经确定刺突蛋白（S1）是早期检测持久性病毒库和潜伏性COVID-19的明确生物标志物。设计了一种集成纳米复合材料的新型三明治式电化学免疫传感器，用于快速灵敏地检测COVID-19潜伏性。该平台结构为AuSPE|| |strep|Nb1|BSA|biotin|S1|strep- lismzro3 -Nb2 + BSA (AuSPE =金丝网印刷电极，strep =链亲和素-硫醇，Nb1 =一级纳米体，Nb2 =二级纳米体，BSA =牛血清白蛋白，spike （S1）蛋白= S1)，其特征是用strep修饰的一次性AuSPE来锚定spike蛋白特异性的生物素化的camelid Nb1。Nb2与链霉亲和素标记的LiSmZrO3钙钛矿结合完成了三明治复合物，增强了亲和力和信号转导。通过电化学发光（ECL）信号转导研究了传感器的电化学响应。对s1敏感的夹心免疫传感器检测范围为0 ~ 1000 pg mL-1， ECL检测限为0.04 pg mL-1。作为可行性研究，缓冲溶液和人血清中的商业刺突蛋白，突出了免疫传感器在SARS-CoV-2患者和pvr中的应用潜力。该纳米体夹心免疫传感器具有良好的稳定性、选择性、灵敏度和可重复性。该免疫传感器可作为一种广泛的PVR，用于SARS-CoV-2筛查工具，检测S1水平升高，从而实现早期、有针对性的诊断。

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引用次数: 0

Exploration and advances in enzymatic electrochemical biosensors for in vivo detection of brain glutamate 脑谷氨酸体内检测酶法电化学生物传感器的探索与进展。

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2026-03-01 Epub Date: 2025-11-03 DOI: 10.1016/j.bioelechem.2025.109161

R. Divya Mohan , Shashanka Rajendrachari , Habdias de Araujo Silva Neto , A. Santhy , R. Rejithamol

The presence of elevated glutamate levels is linked to a variety of neurological disorders, creating an urgent demand for advancements in glutamate detection technologies. This review underscores the major advancements and breakthroughs that have influenced the development of L-glutamate biosensing technologies, showcasing the significant progress made over time. Electrochemical glutamate sensors are widely used for real-time in vivo monitoring of glutamate with high temporal resolution. Electrochemical sensors can detect rapid glutamate changes on the order of seconds or faster, providing sub-second temporal resolution that captures transient neurotransmitter release events. Enzyme-based microsensors have demonstrated detection limits in the low micromolar or hundreds or tens-of-nanomolar range, suitable for physiological extracellular glutamate levels. This review focuses on the advances in enzymatic electrochemical sensors for the neurotransmitter L-Glutamate in brain fluids.

谷氨酸水平升高与多种神经系统疾病有关，因此迫切需要谷氨酸检测技术的进步。本文综述了影响l -谷氨酸生物传感技术发展的主要进展和突破，展示了随着时间的推移取得的重大进展。电化学谷氨酸传感器广泛应用于谷氨酸的体内实时监测，具有较高的时间分辨率。电化学传感器可以以秒级或更快的速度检测谷氨酸的快速变化，提供亚秒级的时间分辨率，捕捉瞬时神经递质释放事件。基于酶的微传感器已证明检测限在低微摩尔或数百或数十纳摩尔范围内，适用于生理细胞外谷氨酸水平。本文综述了脑液中l -谷氨酸神经递质酶电化学传感器的研究进展。

引用次数: 0

Magnetized electrochemically active bacteria-based whole-cell biosensors for real-time sensing of water toxicity 用于水毒性实时传感的磁化电化学活性细菌全细胞生物传感器

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2026-03-01 Epub Date: 2025-09-23 DOI: 10.1016/j.bioelechem.2025.109119

Hongyu Zhao , Yanhong Ge , Jing Wu , Bo Cao , Yue Yi , Beizhen Xie , Hong Liu

Real-time sensing of water toxicity is essential for environmental health monitoring. However, devising an electrochemically active biofilm-based biosensor for water toxicity assaying usually requires cumbersome culture techniques to immobilize electrochemically active bacteria (EAB) on the electrode, which results in poor timeliness of water quality early-warning. Herein, we developed magnetized electrochemically active bacteria (MEAB)-based whole-cell biosensors (WCBs) for real-time sensing of water toxicity. The results showed that artificial MEAB biofilm could be magnetically constructed in one step within 5 s, which greatly simplified the fabrication process of artificial electrochemically active biofilm. By correlating quantifiable bioelectrical signal with MEAB cell activity, the MEAB-based WCBs enabled detection of Hg²⁺, trichloroacetic acid (TCAA), avermectin (AVM), Cr⁶⁺, and chlortetracycline hydrochloride (CTC) in synthetic water samples within 30 min. The estimated detection limit for Hg²⁺, TCAA, AVM, Cr⁶⁺, and CTC reached 50.4 ± 1.6, 54.7 ± 1.5, 62.8 ± 2.0, 66.8 ± 1.6, and 73.3 ± 2.2 μg L⁻¹ with optimal biomass, respectively. As proof-of-concept applications, the MEAB-based WCBs not only achieved accurate detection of 0.1 mg L⁻¹ toxicants in real water samples but also successfully sensed comprehensive toxicity of agricultural wastewater within 30 min. This study provides a new strategy for real-time sensing of water toxicity.

水毒性的实时监测是环境健康监测的必要条件。然而，设计用于水毒性分析的电化学活性生物膜生物传感器通常需要繁琐的培养技术来将电化学活性细菌（EAB）固定在电极上，这导致水质预警的及时性较差。在此，我们开发了基于磁化电化学活性细菌（MEAB）的全细胞生物传感器（WCBs），用于实时检测水毒性。结果表明，MEAB人工生物膜可在5 s内一步磁性构建完成，大大简化了人工电化学活性生物膜的制备过程。通过将可量化的生物电信号与MEAB细胞活性相关联，基于MEAB的wcb能够在30分钟内检测合成水样中的Hg2+、三氯乙酸（TCAA）、阿维菌素（AVM）、Cr6+和盐酸氯四环素（CTC）。在最佳生物量条件下，Hg2+、TCAA、AVM、Cr6+和CTC的检出限分别为50.4±1.6、54.7±1.5、62.8±2.0、66.8±1.6和73.3±2.2 μg L−1。作为概念验证应用，基于meab的wcb不仅实现了对真实水样中0.1 mg L−1毒物的准确检测，而且在30分钟内成功地检测了农业废水的综合毒性。本研究为水毒性实时监测提供了一种新的方法。

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引用次数: 0

Microbial electrosynthesis of acetic acid from carbon dioxide using a bimetallic-granular activated carbon cathode 用双金属颗粒活性炭阴极从二氧化碳中电合成乙酸的微生物研究

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2026-03-01 Epub Date: 2025-11-01 DOI: 10.1016/j.bioelechem.2025.109158

Sadaf Shakeel , Mohammad Zain Khan , Mariya Shakeel

Development of highly stable, conductive, biocompatible and cost-effective cathode is crucial for scaling up microbial electrosynthesis (MES). Bimetallic cathodes have gain importance in recent times due wide application. Current study utilized a bimetallic (nickel and iron) impregnated granular activated carbon (Ni-Fe-GAC) cathode in microbial electrosynthesis. Ni-Fe-GAC cathode was prepared via treating GACs in a mixed solution of nickel chloride and iron sulphate. Deposition of metals on GAC facilitates the improved electron transfer and biofilm formation on the GAC surface, giving 1.4 times higher acetate production than plain GAC (control). Scanning electron microscopy (SEM) confirmed the well adaptation of anaerobic microbes on the surface of Ni-Fe-GAC cathode. Linear sweep voltammetry (LSV) confirmed the better electrochemical performance of Ni-Fe-GAC cathode. A regeneration test was also conducted via acid washing to remove metals from Ni-Fe-GAC followed by reloading. Regeneration successfully restored cathode performance, making Ni-Fe-GAC suitable for long-term application.

开发高稳定性、导电性、生物相容性和高性价比的阴极对于扩大微生物电合成（MES）的规模至关重要。双金属阴极由于其广泛的应用，近年来越来越受到重视。目前的研究利用双金属（镍和铁）浸渍颗粒活性炭（Ni-Fe-GAC）阴极进行微生物电合成。在氯化镍和硫酸铁的混合溶液中处理gac，制备了Ni-Fe-GAC阴极。金属在GAC上的沉积促进了GAC表面的电子转移和生物膜的形成，使GAC的醋酸酯产量比普通GAC（对照组）高1.4倍。扫描电镜（SEM）证实厌氧微生物在Ni-Fe-GAC阴极表面具有良好的适应性。线性扫描伏安法（LSV）证实了Ni-Fe-GAC阴极具有较好的电化学性能。还进行了酸洗再生试验，以去除Ni-Fe-GAC中的金属，然后重新加载。再生成功地恢复了阴极性能，使Ni-Fe-GAC适合长期应用。

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引用次数: 0

Carbon nanotubes/polythiophene composite anodes: Fabrication and electrochemical energy storage performance 碳纳米管/聚噻吩复合阳极：制备及其电化学储能性能。

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2026-03-01 Epub Date: 2025-11-11 DOI: 10.1016/j.bioelechem.2025.109167

Yuyang Wang , Xiangquan Kong , Zhijie Wang , Jinlong Zuo , Dongming Zhang , Su Ma , Yu Song , Yuchu Chen , Guofeng Duan

This study presents a significant advancement in a capacitive carbon felt/carbon nanotube/polythiophene (CF/CNT/PTh) bioanode. The CF framework provides mechanical stability. CNTs form a mesoporous structure (Barrett-Joyner-Halenda (BJH) pore volume: 0.016991 cm³/g), which supports increased populations of electroactive microorganisms. High-throughput sequencing confirmed a 6.67-fold increase in the relative abundance of electroactive genera. The key innovation of the CF/CNT/PTh is the incorporation of PTh to redesign the bioelectrochemical interface. The hydrophobic surface of PTh (confirmed by Fourier-transform infrared spectroscopy and Brunauer-Emmett-Teller analysis) reduces interfacial water barriers. Consequently, the charge-transfer resistance decreases by 65.3 % (R_ct = 2.80 Ω), as measured via electrochemical impedance spectroscopy. Additionally, the pseudocapacitive properties of PTh enable the storage of 5842.55C/m² of charge and generate a stronger bioelectric field (−578 mV open-circuit potential (OCP)), which enhances microbial activity. These effects create an “energy hub” in the bioanode, as stored and real-time electrons merge during discharge. Therefore, the CF/CNT/PTh bioanode achieves a current density of 147.925 A/m² and a power density of 1216.03 mW/m², which is 1.64 times that of bare CF. This design establishes a novel system through a microbial habitat environment constructed with CNT and a polythiophene-enhanced electron transfer mechanism, offering an innovative solution for micropower applications.

本研究提出了电容性碳毡/碳纳米管/聚噻吩（CF/CNT/PTh）生物阳极的重大进展。CF框架提供机械稳定性。CNTs形成介孔结构（Barrett-Joyner-Halenda （BJH）孔体积：0.016991 cm3/g），支持电活性微生物数量的增加。高通量测序证实电活性属的相对丰度增加了6.67倍。CF/CNT/PTh的关键创新在于PTh的加入重新设计了生物电化学界面。PTh的疏水表面（由傅里叶变换红外光谱和布鲁诺尔-埃米特-泰勒分析证实）降低了界面水屏障。因此，通过电化学阻抗谱测量，电荷转移电阻降低了65.3% （Rct = 2.80 Ω）。此外，PTh的赝电容特性使其能够存储5842.55C/m2的电荷，并产生更强的生物电场（-578 mV开路电位（OCP）），从而增强微生物活性。这些效应在生物阳极中创造了一个“能量中心”，因为存储的和实时的电子在放电过程中融合在一起。因此，CF/CNT/PTh生物阳极的电流密度为147.925 a /m2，功率密度为1216.03 mW/m2，是裸CF的1.64倍。本设计通过碳纳米管构建的微生物栖息地环境和多噻吩增强的电子转移机制，建立了一种新的系统，为微功率应用提供了创新的解决方案。

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引用次数: 0

Ratiometric electrochemical sensor based on metal-organic framework and nanosilver as response signals for cTnI detection 基于金属-有机骨架和纳米银作为响应信号的比例电化学传感器用于cTnI检测

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2026-03-01 Epub Date: 2025-09-05 DOI: 10.1016/j.bioelechem.2025.109099

Yanju Liu , Gao Si , Yuning Zhao , Zhixiang Liu , Xiaohua He , Huaixia Yang , Liying Zhao

The concentration of cardiac troponin I (cTnI) serves as a well-established biomarker of myocardial injury. In this work, a ratiometric electrochemical aptasensor utilizing Zirconium-metal organic framework (UiO-66) and AgNPs as electrochemical signaling tags were developed for the ultrasensitive detection of cardiac troponin I (cTnI). Upon specific binding between cTnI and the aptamer, probe 1 (P1) dissociated from the aptamer, resulting in a decreased UiO-66 signal. Meanwhile, signal amplification mediated by terminal deoxynucleotide transferase (TdT) provided a substantial quantity of active sites for surface-initiated reversible addition fragmentation chain transfer (SI-RAFT) polymerization, leading to a massive amount of silver nano-particles deposited on the electrode surfaces with enhanced silver ion signals. The concentration range of cTnI was determined to be 1 × 10⁻³–1 × 10² ng mL⁻¹, with a detection limit as low as 20.53 fg mL⁻¹. Notably, the ratiometric sensor performed well in detecting cTnI in serum samples from patients, indicating clinical application potential. Importantly, the aptasensor exhibited excellent performance in detecting cTnI in human serum samples, highlighting its strong potential for clinical application.

心肌肌钙蛋白I （cTnI）的浓度是心肌损伤的一个公认的生物标志物。在这项工作中，利用锆金属有机框架（UiO-66）和AgNPs作为电化学信号标签，开发了一种比例电化学感应传感器，用于超灵敏检测心脏肌钙蛋白I （cTnI）。在cTnI与适体特异性结合后，探针1 （P1）与适体分离，导致UiO-66信号减弱。同时，末端脱氧核苷酸转移酶（TdT）介导的信号扩增为表面引发的可逆加成断裂链转移（SI-RAFT）聚合提供了大量活性位点，导致大量银纳米粒子沉积在电极表面，银离子信号增强。测定cTnI的浓度范围为1 × 10−3-1 × 102 ng mL−1，检出限低至20.53 fg mL−1。值得注意的是，比例传感器在检测患者血清样本中的cTnI方面表现良好，显示了临床应用潜力。重要的是，该适体传感器在检测人血清样品中的cTnI方面表现出优异的性能，突出了其强大的临床应用潜力。

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引用次数: 0

Development of peroxidase-modified zeolite carbon paste electrodes (Prx-Zeo/CPE) for the biosensing of hydroquinone in pharmaceutical skin cream 过氧化物酶修饰的沸石碳糊电极（Prx-Zeo/CPE）在药用护肤霜中对苯二酚的生物传感研究。

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2026-03-01 Epub Date: 2025-10-22 DOI: 10.1016/j.bioelechem.2025.109150

Roberta C. Martins , Pedro A. da Silva Pereira , Alexandre L.B. Baccaro , Lúcio Angnes , Adelir A. Saczk , Fabiana S. Felix

Peroxidases are oxidoreductase enzymes with intense catalytic activity toward the peroxidation of hydrogen donors in the presence of hydrogen peroxide. These enzymes are appealing for application as the biomolecular recognition step of electrochemical biosensors, since they can be easily extracted from vegetable tissues such as Solanum aethiopicum (popularly known as “Gilo”), considerably reducing the costs of sensing devices. Meanwhile, zeolites are hydrated aluminosilicates arranged in 3D tetrahedral crystalline reticules that form microporous inner structures with high internal and external surface areas, very convenient for the immobilization of high amounts of enzymes to possibly improve the electrochemical biosensors sensitivity. In this study, a peroxidase biosensor utilizing enzymes extracted from raw Gilo tissue was developed. The high adsorption capacity of zeolites (MFI-based NaY) was explored to immobilize the peroxidase enzymes. The extraction efficiency was evaluated using three different types of polymeric protectors: polyvinylpyrrolidone (PVP K90), latex, or polyvinyl alcohol (PVOH. Among them, PVOH was identified as the most effective protector. The peroxidase enzyme was immobilized on NaY zeolite, forming the peroxidase-modified zeolite that was added to carbon paste electrodes to assemble the Prx-Zeo/CPE bioelectrode. The effects of graphite powder, zeolite, mineral oil, and peroxidase concentration in the paste composition were investigated using factorial planning with the premises of the Design of Experiments (DOE) to optimize cathodic current values for hydroquinone detection. Finally, the Prx-Zeo/CPE biosensor was applied to determine the hydroquinone content in two different of skin cream samples. Hydroquinone were determined by direct interpolation of the sample analytical signals onto an analytical curve (0.2–1.0 mmol L⁻¹) and subsequently compared with the label nominal values provided by the manufacturers, as well as with those obtained through parallel analysis using an HPLC reference method.

过氧化物酶是一种氧化还原酶，在过氧化氢存在下对供体氢的过氧化具有强烈的催化活性。这些酶作为电化学生物传感器的生物分子识别步骤很有吸引力，因为它们可以很容易地从植物组织中提取，如埃塞俄比亚茄（俗称“Gilo”），大大降低了传感设备的成本。同时，沸石是水合铝硅酸盐，排列在三维四面体网状晶体中，形成具有高内外表面积的微孔内部结构，非常便于固定化大量酶，可能提高电化学生物传感器的灵敏度。在本研究中，利用从原始Gilo组织中提取的酶开发了过氧化物酶生物传感器。利用高吸附量的沸石（mfi基NaY）固定化过氧化物酶。采用三种不同类型的聚合物保护剂：聚乙烯吡咯烷酮（PVP K90）、乳胶或聚乙烯醇（PVOH）来评估提取效率。其中，PVOH被认为是最有效的保护剂。将过氧化物酶固定在NaY沸石上，形成过氧化物酶修饰的沸石，并将其添加到碳糊电极上组装Prx-Zeo/CPE生物电极。以实验设计（DOE）为前提，研究了石墨粉、沸石、矿物油和过氧化物酶浓度对膏体组成的影响，以优化对苯二酚检测的阴极电流值。最后，应用Prx-Zeo/CPE生物传感器测定两种不同护肤霜样品中对苯二酚的含量。将样品的分析信号直接插值到0.2 ~ 1.0 mmol L-1的分析曲线上，测定对苯二酚的含量，并与生产商提供的标签标称值进行比较，并与HPLC参考法平行分析得到的对苯二酚含量进行比较。

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引用次数: 0

A comprehensive study of nitroxoline – Ct-DNA interaction using electrochemical, spectroscopic, viscometry and thermodynamics as analytical tools 利用电化学、光谱学、粘度学和热力学等分析手段，对硝基喹啉- Ct-DNA相互作用进行了综合研究。

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2026-03-01 Epub Date: 2025-11-11 DOI: 10.1016/j.bioelechem.2025.109163

Umbreen Ashraf , Iqra Saleem , Athar Yaseen Khan , Mariya al Rashida , Safeer Ahmad

Nitroxoline (NXN) is an antibiotic of nitroquinoline family which is used for the treatment of urinary tract infections and as a biofilm eradicating agent. Since it shows potential antitumor activity, it is also considered a promising candidate for repurposing in cancer treatment. Present article reports on electrochemical investigations of nitroxoline, its interaction with ct-DNA using cyclic voltammetry, UV–visible spectroscopy, viscometry, molecular modelling, and thermodynamics as analytical tools. An irreversible reduction peak is observed at about −0.45 V at pH 4.0 which shifts to more negative potentials with increasing scan rate. With the addition of DNA, the signal intensity decreases indicating formation of an adduct which enabled calculation of binding constant

K_{b}

= (9.175

\pm

0.728) × 10⁴ M⁻¹ and 2S = (0.925

\pm

0.150). Spectroscopic measurements yielded a value of (3.366

\pm

0.0.193) × 10⁴ M⁻¹. Viscosity measurements show intercalation binding mode for the drug which is supported by preliminary molecular docking studies. Thermodynamic studies reveal that ∆G° is negative and both ∆H° and ∆S° are positive, indicating spontaneity of the binding process and hydrophobic forces are dominant in binding of the drug. Electrochemical parameters, transfer coefficient (

α

), diffusion coefficient (D₀) and heterogeneous electron transfer rate (

k_{s, h})

obtained for nitroxoline at pH 4.0 indicate mild electron transfer kinetics.

Quinoline structure and nitro group are medicinally important. Present study reports for the first time qualitative and quantitative data on an important member of the quinoline family which would be of interest to researchers engaged in drug development.

硝基喹啉（Nitroxoline， NXN）是硝基喹啉家族的一种抗生素，用于治疗尿路感染和作为生物膜根除剂。由于它显示出潜在的抗肿瘤活性，它也被认为是癌症治疗中有希望的候选物。本文报道了利用循环伏安法、紫外可见光谱法、粘度法、分子模型法和热力学等分析手段对硝基喹啉及其与ct-DNA相互作用的电化学研究。当pH值为4.0时，在-0.45 V处出现不可逆还原峰，随着扫描速率的增加，还原峰向负电位偏移。随着DNA的加入，信号强度降低，表明加合物的形成，结合常数Kb =（9.175±0.728）× 104 M-1, 2S =（0.925±0.150）。光谱测量结果为（3.366±0.0.193）× 104 M-1。粘度测量显示了药物的嵌入结合模式，这得到了初步分子对接研究的支持。热力学研究表明，∆G°为负，∆H°和∆S°均为正，说明结合过程的自发性和疏水性在药物结合中起主导作用。pH为4.0时，硝基喹啉的电化学参数、传递系数（α）、扩散系数（D0）和非均相电子传递速率（ks,h）均显示出温和的电子传递动力学。喹啉结构和硝基具有重要的医学意义。本研究首次报道了喹啉家族重要成员的定性和定量数据，这将对从事药物开发的研究人员感兴趣。

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引用次数: 0

Electrochemical immunosensor based on sea urchin-like PdAg and Au NPs/N-C@CNTs for ultrasensitive detection of CEA 基于海胆样PdAg和Au NPs/N-C@CNTs的电化学免疫传感器用于CEA的超灵敏检测。

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2026-03-01 Epub Date: 2025-09-28 DOI: 10.1016/j.bioelechem.2025.109124

Yang Li , Hongzhu Yan , Zhe Zhao, Ping Wang, Feng Tang, Qingyan Chu, Zhao Yang, Shujun Wang, Qing Liu, Yueyun Li

Carcinoembryonic antigen (CEA), a clinically critical tumor biomarker, enables early cancer screening and diagnosis. Here, we describe a sandwich-structured electrochemical immunosensing platform enabling supersensitive CEA quantification, leveraging synergistic signal amplification by sea urchin-like PdAg nanostructures and Au NPs/N-C@CNTs substrates. The urchin-like morphology of PdAg endows the material additional catalytic active sites for hydrogen peroxide reduction, which has remarkable electrochemical performance. Moreover, PdAg with superior biocompatibility can effectively immobilize the secondary antibody. Polydopamine-coated carbon nanotubes are carbonized to yield nitrogen-doped carbon nanotubes (N-C@CNTs), which are bound to gold nanoparticles (Au NPs) via stable AuN bonds, thereby facilitating the subsequent binding of primary antibodies to the Au NPs. Optimized assays demonstrated a broad dynamic range (50 fg mL⁻¹–100 ng mL⁻¹) with low detection limits (1.04 fg mL⁻¹, S/N = 3), coupled with exceptional reproducibility, selectivity, and stability. This platform holds significant promise for the screening of early-stage tumor biomarkers.

癌胚抗原（CEA）是临床重要的肿瘤生物标志物，可用于早期癌症筛查和诊断。在这里，我们描述了一个三明治结构的电化学免疫传感平台，利用海胆样PdAg纳米结构和Au NPs/N-C@CNTs底物的协同信号放大，实现了超灵敏的CEA定量。PdAg的海胆状形态赋予了材料额外的过氧化氢还原催化活性位点，具有显著的电化学性能。此外，PdAg具有良好的生物相容性，可以有效地固定二抗。将多多巴胺包被的碳纳米管碳化生成氮掺杂碳纳米管（N-C@CNTs），碳纳米管通过稳定的AuN键与金纳米粒子（Au NPs）结合，从而促进了随后一抗与Au NPs的结合。优化后的分析方法具有较宽的动态范围（50 fg mL-1-100 ng mL-1），低检出限（1.04 fg mL-1, S/N = 3），以及出色的重现性、选择性和稳定性。该平台对早期肿瘤生物标志物的筛选具有重要的前景。

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引用次数: 0

Influence of initial cell counts on the microbiologically influenced corrosion of L245N steel in shale gas environments 初始细胞数对页岩气环境中L245N钢微生物腐蚀的影响

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2026-03-01 Epub Date: 2025-11-08 DOI: 10.1016/j.bioelechem.2025.109157

Yanran Wang , Hongfa Huang , Yongfan Tang , Shaomu Wen , Xulin Hou

Microbiologically influenced corrosion (MIC) caused by sulfate-reducing bacteria (SRB) poses a major threat to pipeline integrity in shale gas operations. Current industry standards set a control threshold of 25 cells/mL for SRB, but its scientific validity remains unclear. This study investigates the corrosion behavior of L245N steel under varying initial SRB concentrations (10⁴ to 25 cells/mL) with 50 ppm THPS biocide presence. Results reveal a distinct threshold effect between 10³ and 10² cells/mL, where corrosion severity, biofilm thickness, and sessile cell count drop sharply. Further reductions below 10² cells/mL produce negligible corrosion impact. However, there is no substantial difference between 100, 50, and 25 cells/mL, indicating a plateau effect. Sessile cell counts, biofilm morphology, electrochemical parameters, and corrosion rates all support this finding. Additionally, mixed microbial consortia enhanced SRB survival under biocidal conditions. These results suggest that the current standard may not be sufficient for MIC prevention and highlight the need for revised standards based on corrosion behavior rather than planktonic cell counts alone.

在页岩气作业中，由硫酸盐还原菌（SRB）引起的微生物影响腐蚀（MIC）对管道完整性构成了重大威胁。目前的行业标准为SRB设定了25个细胞/mL的控制阈值，但其科学有效性尚不清楚。本研究研究了L245N钢在不同初始SRB浓度（104 ~ 25 cells/mL）和50 ppm THPS杀菌剂存在下的腐蚀行为。结果显示，在103和102细胞/mL之间存在明显的阈值效应，腐蚀严重程度、生物膜厚度和无根细胞计数急剧下降。进一步降低到102格/毫升以下，腐蚀影响可以忽略不计。然而，在100、50和25个细胞/mL之间没有实质性差异，表明存在平台效应。无孔细胞计数、生物膜形态、电化学参数和腐蚀速率都支持这一发现。此外，混合菌群可提高SRB在杀生物剂条件下的存活率。这些结果表明，目前的标准可能不足以预防MIC，并强调需要根据腐蚀行为修订标准，而不仅仅是浮游细胞计数。

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