Pub Date : 2025-03-13DOI: 10.1016/j.colsurfb.2025.114633
Yixuan Zhu , Qian Wang , Yaoyao Zheng , Nuo Chen , Longfa Kou , Qing Yao
Acute pancreatitis (AP) is an acute inflammation of the pancreas, which is considered a prevalent gastrointestinal emergency characterized by rapid progression and significant mortality. Currently available medications primarily serve as adjunctive therapies, yielding suboptimal therapeutic outcomes. Consequently, there remains a dearth of specific and efficient treatment modalities for AP. In recent years, nanomedicine-based treatment strategies have exhibited significant potential as drug therapy approaches for pancreatitis. The distinctive features of the AP microenvironment encompass aberrant activation of pancreatic enzymes, oxidative stress induced by elevated reactive oxygen species levels, and excessive production of pro-inflammatory cytokines; these factors offer promising targeted sites for early diagnosis and treatment using nanomedicine. This article comprehensively delineates the pathological microenvironmental characteristics associated with AP while highlighting the application of microenvironment-responsive strategies in nanodrug delivery systems for its treatment, thereby providing insights into future prospects.
{"title":"Microenvironment responsive nanomedicine for acute pancreatitis treatment","authors":"Yixuan Zhu , Qian Wang , Yaoyao Zheng , Nuo Chen , Longfa Kou , Qing Yao","doi":"10.1016/j.colsurfb.2025.114633","DOIUrl":"10.1016/j.colsurfb.2025.114633","url":null,"abstract":"<div><div>Acute pancreatitis (AP) is an acute inflammation of the pancreas, which is considered a prevalent gastrointestinal emergency characterized by rapid progression and significant mortality. Currently available medications primarily serve as adjunctive therapies, yielding suboptimal therapeutic outcomes. Consequently, there remains a dearth of specific and efficient treatment modalities for AP. In recent years, nanomedicine-based treatment strategies have exhibited significant potential as drug therapy approaches for pancreatitis. The distinctive features of the AP microenvironment encompass aberrant activation of pancreatic enzymes, oxidative stress induced by elevated reactive oxygen species levels, and excessive production of pro-inflammatory cytokines; these factors offer promising targeted sites for early diagnosis and treatment using nanomedicine. This article comprehensively delineates the pathological microenvironmental characteristics associated with AP while highlighting the application of microenvironment-responsive strategies in nanodrug delivery systems for its treatment, thereby providing insights into future prospects.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"251 ","pages":"Article 114633"},"PeriodicalIF":5.4,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143644647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lung cancer remains a significant global health burden as the second most common and fatal malignancy, with treatment complexities heightened by limited knowledge of inhaler techniques and respiratory challenges, particularly in elderly and pediatric patients. Despite the availability of oral chemotherapeutics like Nintedanib, its clinical efficacy is undermined by suboptimal pharmacokinetics, high systemic toxicity, and low bioavailability. To overcome these limitations, we developed folic acid-conjugated Nintedanib-loaded solid lipid nanoparticles (FA-NIN-SLNPs), which offer targeted therapy with enhanced delivery and reduced adverse effects, potentially improving patient adherence. Prepared through a refined nanoprecipitation and self-assembly method, FA-NIN-SLNPs exhibited a particle size of 220.5 ± 6.08 nm, a zeta potential of 32.1 ± 3.05 mV, and an entrapment efficiency of 98.3 ± 0.80 %. In vitro release studies indicated accelerated drug release at acidic tumor pH, with FA-NIN-SLNPs showing significantly enhanced apoptosis (86.65 %) in A549 lung cancer cells versus NIN-SLNPs (67.65 %) and free drug (23.53 %). Cellular uptake assays highlighted its targeted capabilities, while histopathological and hemolysis assessments confirmed its safety profile. In vivo pharmacokinetic and biodistribution studies further demonstrated superior lung-specific accumulation, positioning this nanoformulation as a promising, safer, and more efficacious approach for targeted lung cancer therapy.
{"title":"Surface-modified nintedanib-loaded solid lipid nanoparticles for effective targeting of non-small cell lung cancer","authors":"Shubhangi Nalawade , Mahavir Narwade , Vishambhar Deshmukh , Nazim Nasir , Shadma Wahab , Prashant Kesharwani , Kavita R. Gajbhiye","doi":"10.1016/j.colsurfb.2025.114622","DOIUrl":"10.1016/j.colsurfb.2025.114622","url":null,"abstract":"<div><div>Lung cancer remains a significant global health burden as the second most common and fatal malignancy, with treatment complexities heightened by limited knowledge of inhaler techniques and respiratory challenges, particularly in elderly and pediatric patients. Despite the availability of oral chemotherapeutics like Nintedanib, its clinical efficacy is undermined by suboptimal pharmacokinetics, high systemic toxicity, and low bioavailability. To overcome these limitations, we developed folic acid-conjugated Nintedanib-loaded solid lipid nanoparticles (FA-NIN-SLNPs), which offer targeted therapy with enhanced delivery and reduced adverse effects, potentially improving patient adherence. Prepared through a refined nanoprecipitation and self-assembly method, FA-NIN-SLNPs exhibited a particle size of 220.5 ± 6.08 nm, a zeta potential of 32.1 ± 3.05 mV, and an entrapment efficiency of 98.3 ± 0.80 %. <em>In vitro</em> release studies indicated accelerated drug release at acidic tumor pH, with FA-NIN-SLNPs showing significantly enhanced apoptosis (86.65 %) in A549 lung cancer cells versus NIN-SLNPs (67.65 %) and free drug (23.53 %). Cellular uptake assays highlighted its targeted capabilities, while histopathological and hemolysis assessments confirmed its safety profile. <em>In vivo</em> pharmacokinetic and biodistribution studies further demonstrated superior lung-specific accumulation, positioning this nanoformulation as a promising, safer, and more efficacious approach for targeted lung cancer therapy.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"251 ","pages":"Article 114622"},"PeriodicalIF":5.4,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143644648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-03-13DOI: 10.1016/j.colsurfb.2025.114632
Xueqing Li , Yulong Yan , Xubo Zhao
Hyaluronic acid (HA), a naturally occurring polysaccharide, is extensively utilized in the biomedical field owing to its excellent biocompatibility. However, assembling HA directly into nanomaterials with tunable stability remains challenging, primarily due to its hydrophilic nature. In this study, we introduce a novel method for inducing HA assembly through in-situ formation of ZnO nanoparticles to develop HA-based nanomaterials, specifically HA/ZnO nanocubes (HA/ZnO NCs). Following doxorubicin (DOX) loading, the DOX-loaded HA/ZnO NCs exhibit remarkable structural stability under normal physiological conditions and demonstrate acid-responsive dissociation within the tumor microenvironment. In vitro results confirm that HA/ZnO NCs possess excellent biocompatibility, while the DOX-loaded HA/ZnO NCs effectively inhibit tumor cell viability. Consequently, the integration of HA and ZnO represents a promising strategy for enhancing HA-based drug delivery systems (DDSs).
{"title":"Construction of hyaluronic acid/ZnO nanocubes and their pH-responsive stability in drug delivery","authors":"Xueqing Li , Yulong Yan , Xubo Zhao","doi":"10.1016/j.colsurfb.2025.114632","DOIUrl":"10.1016/j.colsurfb.2025.114632","url":null,"abstract":"<div><div>Hyaluronic acid (HA), a naturally occurring polysaccharide, is extensively utilized in the biomedical field owing to its excellent biocompatibility. However, assembling HA directly into nanomaterials with tunable stability remains challenging, primarily due to its hydrophilic nature. In this study, we introduce a novel method for inducing HA assembly through in-situ formation of ZnO nanoparticles to develop HA-based nanomaterials, specifically HA/ZnO nanocubes (HA/ZnO NCs). Following doxorubicin (DOX) loading, the DOX-loaded HA/ZnO NCs exhibit remarkable structural stability under normal physiological conditions and demonstrate acid-responsive dissociation within the tumor microenvironment. In vitro results confirm that HA/ZnO NCs possess excellent biocompatibility, while the DOX-loaded HA/ZnO NCs effectively inhibit tumor cell viability. Consequently, the integration of HA and ZnO represents a promising strategy for enhancing HA-based drug delivery systems (DDSs).</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"251 ","pages":"Article 114632"},"PeriodicalIF":5.4,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143644511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-03-13DOI: 10.1016/j.colsurfb.2025.114631
Polina M. Ivantcova , Adilya R. Sungatullina , Kristina V. Pidgirnaya , Maxim P. Nikitin
The application of bioluminescent luciferin-luciferase systems for visualizing and stimulating various processes in living systems is of great interest due to its specific nature and high signal-to-noise ratio. Nanomaterials can finely manipulate multiple parameters of the bioluminescent systems, including the enzyme stability, intensity, and duration of the irradiation. Also, bioluminescence can affect the properties of a nanomaterial, namely, to carry out BRET, to trigger cascades of various photochemical transformations. Here we summarize cases of the interplay between nanomaterials and various bioluminescent systems to improve various biosensors, biovisualization in cellulo, in vivo, and for therapy over the past twenty years. We reviewed interactions between a wide range of nanomaterials and bioluminescent systems, including bacterial and genetically encoded luciferases. This review aims to serve as a comprehensive guide for developing bioluminescent multimodal nanoplatforms for analytic applications and therapy.
{"title":"Exploring the synergy between bioluminescence and nanomaterials: Innovations in analytical and therapeutic applications","authors":"Polina M. Ivantcova , Adilya R. Sungatullina , Kristina V. Pidgirnaya , Maxim P. Nikitin","doi":"10.1016/j.colsurfb.2025.114631","DOIUrl":"10.1016/j.colsurfb.2025.114631","url":null,"abstract":"<div><div>The application of bioluminescent luciferin-luciferase systems for visualizing and stimulating various processes in living systems is of great interest due to its specific nature and high signal-to-noise ratio. Nanomaterials can finely manipulate multiple parameters of the bioluminescent systems, including the enzyme stability, intensity, and duration of the irradiation. Also, bioluminescence can affect the properties of a nanomaterial, namely, to carry out BRET, to trigger cascades of various photochemical transformations. Here we summarize cases of the interplay between nanomaterials and various bioluminescent systems to improve various biosensors, biovisualization <em>in cellulo</em>, <em>in vivo</em>, and for therapy over the past twenty years. We reviewed interactions between a wide range of nanomaterials and bioluminescent systems, including bacterial and genetically encoded luciferases. This review aims to serve as a comprehensive guide for developing bioluminescent multimodal nanoplatforms for analytic applications and therapy.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"251 ","pages":"Article 114631"},"PeriodicalIF":5.4,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143687758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-03-12DOI: 10.1016/j.colsurfb.2025.114635
Zhiyuan Shao , Hao Wang , Qi Chen , Qihui Zhou , Mingliang Jin , Shoushi Wang , Xuewei Li
As a smart product with flexible, stretchable, and multifunctional sensing properties, electronic skin (e-skin) has made significant research progress in many fields, such as robotics, medical monitoring, and human-computer interaction. However, the current e-skin still has obvious deficiencies in simulating pain perception, especially the limitation in simulating human pain grading perception. In this study, the theory of medical pain grading is applied to the field of e-skin for the first time, and an e-skin system with gradable human-like pain perception is proposed. The system comprises a sensor perception module, an information acquisition module, a neural network processing module, and a visualization feedback module. By combining the electronic skin with neural network technology, the system not only realizes the basic pain grading function but also can adjust the adaptive pain perception according to the environmental temperature change. This study provides a feasible solution for developing graded human-like pain-sensing technology in the fields of intelligent prosthetics, medical monitoring, and human-computer interaction.
{"title":"Graded anthropomorphic pain perception electronic skin","authors":"Zhiyuan Shao , Hao Wang , Qi Chen , Qihui Zhou , Mingliang Jin , Shoushi Wang , Xuewei Li","doi":"10.1016/j.colsurfb.2025.114635","DOIUrl":"10.1016/j.colsurfb.2025.114635","url":null,"abstract":"<div><div>As a smart product with flexible, stretchable, and multifunctional sensing properties, electronic skin (e-skin) has made significant research progress in many fields, such as robotics, medical monitoring, and human-computer interaction. However, the current e-skin still has obvious deficiencies in simulating pain perception, especially the limitation in simulating human pain grading perception. In this study, the theory of medical pain grading is applied to the field of e-skin for the first time, and an e-skin system with gradable human-like pain perception is proposed. The system comprises a sensor perception module, an information acquisition module, a neural network processing module, and a visualization feedback module. By combining the electronic skin with neural network technology, the system not only realizes the basic pain grading function but also can adjust the adaptive pain perception according to the environmental temperature change. This study provides a feasible solution for developing graded human-like pain-sensing technology in the fields of intelligent prosthetics, medical monitoring, and human-computer interaction.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"251 ","pages":"Article 114635"},"PeriodicalIF":5.4,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143642936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-03-12DOI: 10.1016/j.colsurfb.2025.114621
Manas Ranjan Sahu, Akiko Yamamoto
Current in vitro test fails in predicting the in vivo corrosion behaviour of Mg and its alloys. The diffusion of ions and gases through the tissue remains the critical factor influencing the discrepancy between the in vitro and in vivo corrosion rates. To overcome this, the in vitro model tissue with different diffusion rate was developed by the addition of appropriate concentrations of a thickener to the cell culture medium. The corrosion behaviour of WE43 and AZ31 alloys were analysed by immersion studies up to 28 days, electrochemical impedance spectroscopy, and potentiodynamic polarization studies. Both the immersion and electrochemical tests demonstrated the decrease in the corrosion rate of Mg alloys by the addition of thickener. The corrosion rate of WE43 decreased with increase in the thickener concentration whereas those of AZ31 was not obviously influenced by the thickener concentration. This low susceptibility of AZ31 against the change in diffusion rate might be attributed to its slower charge transfer process, as confirmed by the smaller Icorr and larger Rct values. Immersion in the model tissue reduced Ca and P concentrations in the insoluble salt layer on WE43. The lower susceptibility of AZ31 in the model tissue suggests the stability of AZ31 corrosion behaviour in the different tissue with different diffusion rate, which even derives from the difference in the individual patient's pathological condition. The developed model tissue provides an important information on the biocorrosion behaviour of various Mg alloys in consideration of biodegradable implant application.
{"title":"Effect of diffusion in the model tissue on biocorrosion of Mg alloys","authors":"Manas Ranjan Sahu, Akiko Yamamoto","doi":"10.1016/j.colsurfb.2025.114621","DOIUrl":"10.1016/j.colsurfb.2025.114621","url":null,"abstract":"<div><div>Current <em>in vitro</em> test fails in predicting the <em>in vivo</em> corrosion behaviour of Mg and its alloys. The diffusion of ions and gases through the tissue remains the critical factor influencing the discrepancy between the <em>in vitro</em> and <em>in vivo</em> corrosion rates. To overcome this, the <em>in vitro</em> model tissue with different diffusion rate was developed by the addition of appropriate concentrations of a thickener to the cell culture medium. The corrosion behaviour of WE43 and AZ31 alloys were analysed by immersion studies up to 28 days, electrochemical impedance spectroscopy, and potentiodynamic polarization studies. Both the immersion and electrochemical tests demonstrated the decrease in the corrosion rate of Mg alloys by the addition of thickener. The corrosion rate of WE43 decreased with increase in the thickener concentration whereas those of AZ31 was not obviously influenced by the thickener concentration. This low susceptibility of AZ31 against the change in diffusion rate might be attributed to its slower charge transfer process, as confirmed by the smaller <em>I</em><sub><em>corr</em></sub> and larger <em>R</em><sub><em>ct</em></sub> values. Immersion in the model tissue reduced Ca and P concentrations in the insoluble salt layer on WE43. The lower susceptibility of AZ31 in the model tissue suggests the stability of AZ31 corrosion behaviour in the different tissue with different diffusion rate, which even derives from the difference in the individual patient's pathological condition. The developed model tissue provides an important information on the biocorrosion behaviour of various Mg alloys in consideration of biodegradable implant application.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"251 ","pages":"Article 114621"},"PeriodicalIF":5.4,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143642933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-03-12DOI: 10.1016/j.colsurfb.2025.114634
Xiaofeng Liu , Chaojing Li , Fan Zhao , Robert Guidoin , Kaitai Liu , Fujun Wang , Lu Wang
Stent implantation is a widely used palliative treatment for relieving strictures in malignant esophageal cancer. However, conventional fully covered stents play a role in preventing the tumor from ingrowth but increasing the risk of migration. Therefore, there is an urgent need for a multifunctional stent that not only reduces migration but also provides synergistic therapeutic benefits to inhibit tumor growth. This study proposed a braided esophageal stent with synergistic photothermal and chemotherapy functions to achieve precise controllable drug release. The stent was braided of Nitinol fiber and polyethylene terephthalate fiber in one-step, in which Nitinol loaded with high-efficiency photothermal conversion agent gold nanoparticles and polyethylene terephthalate loaded anti-tumor drugs release mediated by a temperature-responsive coating. The stent showed anti-migration ability and orchestrated the localized hyperthermia plus thermal-stimuli drug release during the tumor lesion. Cell experiments confirmed that the stent showed a significantly synergistic tumor cell killing effect (28.29 %). In 3D tumor sphere model, the apoptosis rate of tumor cells reached 31.34 %. In summary, the composite stent design strategy integrates anti-migration features and photoheating-controlled drug release for synergistic cancer therapy, providing a new design idea for the application of nickel-titanium alloy stents in the treatment of malignant esophageal stenosis.
{"title":"A functional stent with near-infrared light triggered localized photothermal-chemo synergistic therapy for malignant stenosis of esophageal cancer","authors":"Xiaofeng Liu , Chaojing Li , Fan Zhao , Robert Guidoin , Kaitai Liu , Fujun Wang , Lu Wang","doi":"10.1016/j.colsurfb.2025.114634","DOIUrl":"10.1016/j.colsurfb.2025.114634","url":null,"abstract":"<div><div>Stent implantation is a widely used palliative treatment for relieving strictures in malignant esophageal cancer. However, conventional fully covered stents play a role in preventing the tumor from ingrowth but increasing the risk of migration. Therefore, there is an urgent need for a multifunctional stent that not only reduces migration but also provides synergistic therapeutic benefits to inhibit tumor growth. This study proposed a braided esophageal stent with synergistic photothermal and chemotherapy functions to achieve precise controllable drug release. The stent was braided of Nitinol fiber and polyethylene terephthalate fiber in one-step, in which Nitinol loaded with high-efficiency photothermal conversion agent gold nanoparticles and polyethylene terephthalate loaded anti-tumor drugs release mediated by a temperature-responsive coating. The stent showed anti-migration ability and orchestrated the localized hyperthermia plus thermal-stimuli drug release during the tumor lesion. Cell experiments confirmed that the stent showed a significantly synergistic tumor cell killing effect (28.29 %). In 3D tumor sphere model, the apoptosis rate of tumor cells reached 31.34 %. In summary, the composite stent design strategy integrates anti-migration features and photoheating-controlled drug release for synergistic cancer therapy, providing a new design idea for the application of nickel-titanium alloy stents in the treatment of malignant esophageal stenosis.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"251 ","pages":"Article 114634"},"PeriodicalIF":5.4,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143632021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-03-11DOI: 10.1016/j.colsurfb.2025.114611
Jin Li , Jiankun Wang , Jin Wu , Xiaoling Wang
During the expansion of Bacillus subtilis biofilm on a solid MSgg substrate, cells within the biofilm form highly organized structures through interactions, growth and differentiation. This organized structure evolves from an initial single chain to bundles known as “Van Gogh bundles,” which guild the biofilm' expansion. In this paper, we present a model for biofilm growth based on cell interaction forces. In this model, cell interactions within Van Gogh bundles are represented by spring connections, and the interactions between Van Gogh bundles and other phenotypic cells are confined to a specific region (repulsive inside the region, attractive outside it). In a single-biofilm system, as nutrients are depleted, increasing numbers of motile cells transform into matrix-producing cells, forming Van Gogh bundles that guide the biofilm expansion towards areas with higher nutrient concentrations, thereby enhancing its expansion ability. In a muti-biofilm system, extreme nutrient depletion leads to the transformation of matrix-producing cells into spores, which affects the number and folding characteristics of Van Gogh bundles, thereby influencing the biofilm expansion. Our study illustrates how the simple organization of cells within a community can provide a significant ecological advantage.
{"title":"Bacillus subtilis biofilm expansion mediated by the interaction between matrix-producing cells formed “Van Gogh bundles” and other phenotypic cells","authors":"Jin Li , Jiankun Wang , Jin Wu , Xiaoling Wang","doi":"10.1016/j.colsurfb.2025.114611","DOIUrl":"10.1016/j.colsurfb.2025.114611","url":null,"abstract":"<div><div>During the expansion of <em>Bacillus subtilis</em> biofilm on a solid MSgg substrate, cells within the biofilm form highly organized structures through interactions, growth and differentiation. This organized structure evolves from an initial single chain to bundles known as “Van Gogh bundles,” which guild the biofilm' expansion. In this paper, we present a model for biofilm growth based on cell interaction forces. In this model, cell interactions within Van Gogh bundles are represented by spring connections, and the interactions between Van Gogh bundles and other phenotypic cells are confined to a specific region (repulsive inside the region, attractive outside it). In a single-biofilm system, as nutrients are depleted, increasing numbers of motile cells transform into matrix-producing cells, forming Van Gogh bundles that guide the biofilm expansion towards areas with higher nutrient concentrations, thereby enhancing its expansion ability. In a muti-biofilm system, extreme nutrient depletion leads to the transformation of matrix-producing cells into spores, which affects the number and folding characteristics of Van Gogh bundles, thereby influencing the biofilm expansion. Our study illustrates how the simple organization of cells within a community can provide a significant ecological advantage.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"251 ","pages":"Article 114611"},"PeriodicalIF":5.4,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143610074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-03-11DOI: 10.1016/j.colsurfb.2025.114629
Jia Zhang , Xianbao Shi , Zhuo Wu
Rheumatoid arthritis (RA) is a chronic autoimmune disease characterized by joint inflammation, pain, and progressive joint damage. Current treatments, while effective, are limited by their potential side effects, particularly in long-term use. This study introduces a novel nanoemulsion-based therapeutic approach combining rapamycin, an mTOR inhibitor, with SS31, a mitochondrial-targeting antioxidant peptide. The rapamycin-SS31 conjugate (RS31) is encapsulated within a nanoemulsion (RS31@NEs) designed to selectively release its components in response to elevated Caspase-3 levels, prevalent in inflamed joints. In vitro and in vivo studies using zymosan-induced arthritis (ZIA) and collagen-induced arthritis (CIA) mouse models demonstrated that RS31@NEs effectively reduced pro-inflammatory cytokines, mitigated oxidative stress, and improved immune modulation by enhancing regulatory T and B cell functions. These findings highlight RS31@NEs as a promising dual-action therapy for RA, combining anti-inflammatory and mitochondrial protective effects while minimizing systemic toxicity.
{"title":"A Caspase-3 responsive nanoemulsion for targeted treatment of rheumatoid arthritis through dual modulation of inflammation and mitochondrial dysfunction","authors":"Jia Zhang , Xianbao Shi , Zhuo Wu","doi":"10.1016/j.colsurfb.2025.114629","DOIUrl":"10.1016/j.colsurfb.2025.114629","url":null,"abstract":"<div><div>Rheumatoid arthritis (RA) is a chronic autoimmune disease characterized by joint inflammation, pain, and progressive joint damage. Current treatments, while effective, are limited by their potential side effects, particularly in long-term use. This study introduces a novel nanoemulsion-based therapeutic approach combining rapamycin, an mTOR inhibitor, with SS31, a mitochondrial-targeting antioxidant peptide. The rapamycin-SS31 conjugate (RS31) is encapsulated within a nanoemulsion (RS31@NEs) designed to selectively release its components in response to elevated Caspase-3 levels, prevalent in inflamed joints. In vitro and in vivo studies using zymosan-induced arthritis (ZIA) and collagen-induced arthritis (CIA) mouse models demonstrated that RS31@NEs effectively reduced pro-inflammatory cytokines, mitigated oxidative stress, and improved immune modulation by enhancing regulatory T and B cell functions. These findings highlight RS31@NEs as a promising dual-action therapy for RA, combining anti-inflammatory and mitochondrial protective effects while minimizing systemic toxicity.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"251 ","pages":"Article 114629"},"PeriodicalIF":5.4,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143628192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-03-11DOI: 10.1016/j.colsurfb.2025.114618
Huanhuan Zhu , Zhenzhen Wang , Jingru Cui , Yaning Ge , Min Yan , Xiangxiang Wu , Xiaofang Li , Huahui Zeng
Triptolide (TP, an active ingredient from Tripterygium wilfordii) demonstrates significant efficacy in treating rheumatoid arthritis, but its low bioavailability and multi-organ toxicity limit its application. Herein, we developed a PEGylated retinoate self-assembled nanomicelles (FA-TP@VA NPs) modified with folic acid (FA) for inflammatory macrophage-targeted delivery of TP. FA-TP@VA NPs showed an appropriate size (192.70 ± 4.37 nm), good physical stability and high drug loading (79.83 ± 5.11 % for retinoate prodrug and 6.78 ± 0.13 % for TP). FA-TP@VA NPs exhibited high cellular uptake in M1 macrophages via folate-receptor pathway, thereby inhibiting their growth. Compared with TP, FA-TP@VA NPs could effectively inhibit synovitis and erosion of bone and reduce swelling and deformation of paws by downregulation of the levels of IL-1β, IL-6, and TNF-α. In ICA mice, FA-TP@VA NPs could enhance drug-specific enrichment in inflamed joints, effectively suppress hepatic oxidative stress and reduce systemic toxicity induced by TP. Overall, FA-TP@VA NPs are a facile, carrier-free, and promising strategy for the precise treatment of rheumatoid arthritis and synergetic attenuation of side effect.
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