Pub Date : 2025-12-11DOI: 10.1016/j.colsurfb.2025.115364
Qing Han , Jie Wang , Lin Long , Ning Wang , Jun Xiao
Plant-derived compounds hold unique advantages in cancer treatment, owing to multi-target synergy and low toxicity, but their clinical translation is often hampered by poor water solubility and low bioavailability. Inspired by the booming innovations in nanotheranostics, formulating these compounds into nanomedicines has emerged as a promising solution. Carrier-free nanomedicines, formed through the self-assembly of drug molecules themselves, represent a cutting-edge research direction since a theoretically 100 % drug-loading ratio can be achieved and carrier-related safety concerns is eliminated by not using foreign inactive ingredients. This review summarizes recent progresses in this specific field, by categorizing the relevant nanomedicines mainly into mono-drug self-assemblies and multidrug co-assemblies. Particular emphasis has been placed on the assembly driving forces for different molecules to facilitate predictive nanoformulation design. Perspectives on the future developments are also given by outlining the critical challenges and potential solutions, and by answering the question of whether the carrier-free strategy is for recognition or for utility. It is anticipated that this overview can provide fundamental insights and catalyze innovative methodologies for the future design of plant-derived nanomedicines.
{"title":"Carrier-free antitumor nanomedicines based on plant-derived compounds: From conceptual innovation to therapeutic potential","authors":"Qing Han , Jie Wang , Lin Long , Ning Wang , Jun Xiao","doi":"10.1016/j.colsurfb.2025.115364","DOIUrl":"10.1016/j.colsurfb.2025.115364","url":null,"abstract":"<div><div>Plant-derived compounds hold unique advantages in cancer treatment, owing to multi-target synergy and low toxicity, but their clinical translation is often hampered by poor water solubility and low bioavailability. Inspired by the booming innovations in nanotheranostics, formulating these compounds into nanomedicines has emerged as a promising solution. Carrier-free nanomedicines, formed through the self-assembly of drug molecules themselves, represent a cutting-edge research direction since a theoretically 100 % drug-loading ratio can be achieved and carrier-related safety concerns is eliminated by not using foreign inactive ingredients. This review summarizes recent progresses in this specific field, by categorizing the relevant nanomedicines mainly into mono-drug self-assemblies and multidrug co-assemblies. Particular emphasis has been placed on the assembly driving forces for different molecules to facilitate predictive nanoformulation design. Perspectives on the future developments are also given by outlining the critical challenges and potential solutions, and by answering the question of whether the carrier-free strategy is for recognition or for utility. It is anticipated that this overview can provide fundamental insights and catalyze innovative methodologies for the future design of plant-derived nanomedicines.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"259 ","pages":"Article 115364"},"PeriodicalIF":5.6,"publicationDate":"2025-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145733394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Recently, siRNA delivery has shown considerable potential for therapeutic use. While commercial options facilitate efficient delivery, they can be toxic to cells to a certain degree. Therefore, using relatively less toxic and safe delivery vehicles can increase the efficiency of therapy. This study examines the utilization of Pullulan–poly(ethyleneimine) nanoparticles (Pullulan-PEI-NPs) as an effective non-viral delivery system for Kelch-like ECH-associated protein 1 (KEAP1) siRNA, with the aims of mitigating oxidative stress and facilitating wound healing in HaCaT cells by enhancing cellular antioxidant mechanisms. The Pullulan-PEI-NPs were synthesized and characterized with Dynamic Light Scattering, Nanoparticle Tracking Analysis, Fourier Transform Infrared Spectroscopy, and electron microscopy techniques. The polyplexes were formed via electrostatic interaction between Pullulan-PEI-NPs and KEAP1 siRNA, and successful siRNA delivery was confirmed by cellular uptake analysis, mRNA expression studies, and KEAP1 and nuclear factor E2-related factor 2 (NRF2) protein level assessments. Pullulan-PEI-NPs had a silencing efficiency of 72 %, which is very close to the commercial transfection agent Lipofectamine 3000 (80 %). In following studies, KEAP1 siRNA delivery with Pullulan-PEI-NPs mitigates TBHP and H2O2 mediated ROS and facilitates wound healing with 98.9 % wound closure in a scratch assay on HaCaT cells. Obtained results highlight the therapeutic relevance of KEAP1 silencing in redox-regulated wound regeneration. This is the first report showing that Pullulan-PEI based cationic polymeric nanoparticles can be used to deliver KEAP1 siRNA and evaluate its role in mitigating ROS and increasing wound healing. These results highlight the promise of Pullulan-PEI-NPs as a safe and adaptable platform for non-viral gene therapy applications.
{"title":"Pullulan-based polymeric nanosystem for KEAP1 siRNA delivery: Mitigating ROS and promoting wound healing in vitro","authors":"Gülşen Bayrak, Dilara Karaguzel, Cagatay Karaaslan, Işık Perçin","doi":"10.1016/j.colsurfb.2025.115352","DOIUrl":"10.1016/j.colsurfb.2025.115352","url":null,"abstract":"<div><div>Recently, siRNA delivery has shown considerable potential for therapeutic use. While commercial options facilitate efficient delivery, they can be toxic to cells to a certain degree. Therefore, using relatively less toxic and safe delivery vehicles can increase the efficiency of therapy. This study examines the utilization of Pullulan–poly(ethyleneimine) nanoparticles (Pullulan-PEI-NPs) as an effective non-viral delivery system for Kelch-like ECH-associated protein 1 (KEAP1) siRNA, with the aims of mitigating oxidative stress and facilitating wound healing in HaCaT cells by enhancing cellular antioxidant mechanisms. The Pullulan-PEI-NPs were synthesized and characterized with Dynamic Light Scattering, Nanoparticle Tracking Analysis, Fourier Transform Infrared Spectroscopy, and electron microscopy techniques. The polyplexes were formed via electrostatic interaction between Pullulan-PEI-NPs and KEAP1 siRNA, and successful siRNA delivery was confirmed by cellular uptake analysis, mRNA expression studies, and KEAP1 and nuclear factor E2-related factor 2 (NRF2) protein level assessments. Pullulan-PEI-NPs had a silencing efficiency of 72 %, which is very close to the commercial transfection agent Lipofectamine 3000 (80 %). In following studies, KEAP1 siRNA delivery with Pullulan-PEI-NPs mitigates TBHP and H<sub>2</sub>O<sub>2</sub> mediated ROS and facilitates wound healing with 98.9 % wound closure in a scratch assay on HaCaT cells. Obtained results highlight the therapeutic relevance of KEAP1 silencing in redox-regulated wound regeneration. This is the first report showing that Pullulan-PEI based cationic polymeric nanoparticles can be used to deliver KEAP1 siRNA and evaluate its role in mitigating ROS and increasing wound healing. These results highlight the promise of Pullulan-PEI-NPs as a safe and adaptable platform for non-viral gene therapy applications.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"260 ","pages":"Article 115352"},"PeriodicalIF":5.6,"publicationDate":"2025-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145792840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-11DOI: 10.1016/j.colsurfb.2025.115366
Tao Qin , Ancan Hong , Yi Lin , Zijie Zheng , Guilan Fan , Chunyan Chen , Zhe Ren
In this study, we developed biomimetic Hericium erinaceus polysaccharides PLGA nanoparticles (NPs) coated with macrophage membrane (MM-PLGA-HEP) and examined their immune activity in vitro and in vivo. Nanoparticle characterization revealed that from HEP to MM-PLGA-HEP, there was an increase in negative surface charge (with enhanced stability), structural changes, and a morphological transition from porous HEP sheets to spherical MM-PLGA-HEP. In vitro evaluations confirmed that MM-PLGA-HEP exhibited no cytotoxicity, significantly enhanced macrophage proliferation, dose-dependently stimulated nitric oxide secretion, and uptake studies confirmed its superior internalization efficiency in macrophages. In vivo administration of MM-PLGA-HEP in mice revealed significant improvements in spleen and liver indices, splenic lymphocyte proliferation, macrophage phagocytosis, and CD40/CD86 expression, indicating robust immune activation. Furthermore, MM-PLGA-HEP induced sustained antigen release, resulting in elevated IgG levels and enhanced humoral immune responses compared to controls. These findings underscore the potential of MM-PLGA-HEP as a novel nanocarrier for targeted drug delivery and vaccine adjuvant applications, providing a promising platform for enhanced immunotherapy and antigen-specific delivery.
{"title":"Physicochemical properties and immunological effects of macrophage membrane-coated PLGA nanoparticles loaded with Hericium erinaceus polysaccharides","authors":"Tao Qin , Ancan Hong , Yi Lin , Zijie Zheng , Guilan Fan , Chunyan Chen , Zhe Ren","doi":"10.1016/j.colsurfb.2025.115366","DOIUrl":"10.1016/j.colsurfb.2025.115366","url":null,"abstract":"<div><div>In this study, we developed biomimetic <em>Hericium erinaceus</em> polysaccharides PLGA nanoparticles (NPs) coated with macrophage membrane (MM-PLGA-HEP) and examined their immune activity <em>in vitro</em> and <em>in vivo.</em> Nanoparticle characterization revealed that from HEP to MM-PLGA-HEP, there was an increase in negative surface charge (with enhanced stability), structural changes, and a morphological transition from porous HEP sheets to spherical MM-PLGA-HEP. <em>In vitro</em> evaluations confirmed that MM-PLGA-HEP exhibited no cytotoxicity, significantly enhanced macrophage proliferation, dose-dependently stimulated nitric oxide secretion, and uptake studies confirmed its superior internalization efficiency in macrophages. <em>In vivo</em> administration of MM-PLGA-HEP in mice revealed significant improvements in spleen and liver indices, splenic lymphocyte proliferation, macrophage phagocytosis, and CD40/CD86 expression, indicating robust immune activation. Furthermore, MM-PLGA-HEP induced sustained antigen release, resulting in elevated IgG levels and enhanced humoral immune responses compared to controls. These findings underscore the potential of MM-PLGA-HEP as a novel nanocarrier for targeted drug delivery and vaccine adjuvant applications, providing a promising platform for enhanced immunotherapy and antigen-specific delivery.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"259 ","pages":"Article 115366"},"PeriodicalIF":5.6,"publicationDate":"2025-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145733392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-11DOI: 10.1016/j.colsurfb.2025.115367
Jiayang Li, Tianyi Wang, Hao Chen, Xiaotong Zhou, Shujun Wang
Effective posterior eye gene delivery remains challenging for neovascular AMD treatment. We developed an intravitreal core-shell nanocarrier with a cationic PβAE dendrimer core (for DNA condensation/endosomal escape), biodegradable PLGA shell (sustained release), and surface HA (vitreal mobility/CD44 targeting). Optimized HA-PP/ND exhibited a uniform size (∼200 nm), high DNA encapsulation efficiency (98 %), and sustained release. In ARPE-19 cells, HA enhanced uptake, lysosomal escape, and transfection efficiency (4.3-fold higher luciferase expression). The carrier delivered antiangiogenic pFlt23K-EGFP, which suppressed VEGF by 48.5 % under hypoxia and inhibited endothelial migration/tube formation. Intravitreally administered HA-PP/N/D reduced CNV lesions by 68 % in a laser-induced wAMD mouse model. This multifunctional platform overcomes ocular gene delivery barriers, providing a promising non-viral strategy for treating posterior segment diseases.
{"title":"Hyaluronic acid-modified core-shell structured cationic nanocarriers for posterior segment gene therapy","authors":"Jiayang Li, Tianyi Wang, Hao Chen, Xiaotong Zhou, Shujun Wang","doi":"10.1016/j.colsurfb.2025.115367","DOIUrl":"10.1016/j.colsurfb.2025.115367","url":null,"abstract":"<div><div>Effective posterior eye gene delivery remains challenging for neovascular AMD treatment. We developed an intravitreal core-shell nanocarrier with a cationic PβAE dendrimer core (for DNA condensation/endosomal escape), biodegradable PLGA shell (sustained release), and surface HA (vitreal mobility/CD44 targeting). Optimized HA-P<sub>P/ND</sub> exhibited a uniform size (∼200 nm), high DNA encapsulation efficiency (98 %), and sustained release. In ARPE-19 cells, HA enhanced uptake, lysosomal escape, and transfection efficiency (4.3-fold higher luciferase expression). The carrier delivered antiangiogenic pFlt23K-EGFP, which suppressed VEGF by 48.5 % under hypoxia and inhibited endothelial migration/tube formation. Intravitreally administered HA-P<sub>P/N/D</sub> reduced CNV lesions by 68 % in a laser-induced wAMD mouse model. This multifunctional platform overcomes ocular gene delivery barriers, providing a promising non-viral strategy for treating posterior segment diseases.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"259 ","pages":"Article 115367"},"PeriodicalIF":5.6,"publicationDate":"2025-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145733388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-10DOI: 10.1016/j.colsurfb.2025.115351
Yuhan Zhang , Wenjun Shu , Yijun Han , Zeinab Marfavi , Quanjie Lv , Yuruo Zhang , Congli Yuan , Ke Tao
Sonodynamic therapy (SDT), due to its non-invasive nature, high tissue penetration, and lack of drug resistance, has shown significant application prospects in the field of clinical antibacterial treatment. However, existing studies often require high ultrasonic energy, long ultrasonic exposure time, and high concentrations of sonosensitizer materials because the production of reactive oxygen species (ROS) by the sonosensitizers is typically insufficient. In this study, we showed that thulium oxide nanoparticles (Tm2O3 NPs) could be a candidate sonosensitizer exhibiting superior ROS production. We adjusted the ratio of surfactants in the synthesis system, tested the production of various types of ROS under ultrasonic irradiation, and studied the antibacterial activity of thulium oxide nanoparticles against gram-negative Escherichia coli (E. coli) and gram-positive Staphylococcus aureus (S. aureus). Under ultrasonic action, thulium oxide nanoparticles can produce singlet oxygen (1O2) and hydroxyl radicals (·OH), with type I ROS (radical type) being the primary form of ROS produced. We demonstrated that the thulium oxide nanoparticles exhibited apparent antibacterial activity against E. coli and S. aureus under ultrasonic irradiation and promoted the healing of bacterial-infected wounds. This study suggested a novel rare-earth nanomaterial sonosensitizer with outstanding ROS yield and clinical practicality, providing an energy-efficient and material-saving alternative to classical sonodynamic and photodynamic therapies.
{"title":"Ultrasound-triggered reactive oxygen species production and antibacterial activity by thulium oxide nanoparticles","authors":"Yuhan Zhang , Wenjun Shu , Yijun Han , Zeinab Marfavi , Quanjie Lv , Yuruo Zhang , Congli Yuan , Ke Tao","doi":"10.1016/j.colsurfb.2025.115351","DOIUrl":"10.1016/j.colsurfb.2025.115351","url":null,"abstract":"<div><div>Sonodynamic therapy (SDT), due to its non-invasive nature, high tissue penetration, and lack of drug resistance, has shown significant application prospects in the field of clinical antibacterial treatment. However, existing studies often require high ultrasonic energy, long ultrasonic exposure time, and high concentrations of sonosensitizer materials because the production of reactive oxygen species (ROS) by the sonosensitizers is typically insufficient. In this study, we showed that thulium oxide nanoparticles (Tm<sub>2</sub>O<sub>3</sub> NPs) could be a candidate sonosensitizer exhibiting superior ROS production. We adjusted the ratio of surfactants in the synthesis system, tested the production of various types of ROS under ultrasonic irradiation, and studied the antibacterial activity of thulium oxide nanoparticles against gram-negative Escherichia coli (<em>E. coli</em>) and gram-positive Staphylococcus aureus (<em>S. aureus</em>). Under ultrasonic action, thulium oxide nanoparticles can produce singlet oxygen (<sup>1</sup>O<sub>2</sub>) and hydroxyl radicals (·OH), with type I ROS (radical type) being the primary form of ROS produced. We demonstrated that the thulium oxide nanoparticles exhibited apparent antibacterial activity against <em>E. coli</em> and <em>S. aureus</em> under ultrasonic irradiation and promoted the healing of bacterial-infected wounds. This study suggested a novel rare-earth nanomaterial sonosensitizer with outstanding ROS yield and clinical practicality, providing an energy-efficient and material-saving alternative to classical sonodynamic and photodynamic therapies.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"259 ","pages":"Article 115351"},"PeriodicalIF":5.6,"publicationDate":"2025-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145733385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The increasing prevalence of UV-induced skin damage and the limitations of synthetic sunscreens, such as toxicity, environmental harm, and inadequate multifunctionality, have driven demand for natural, biocompatible alternatives. Fenugreek (Trigonella foenum-graecum) seed extract (FE), rich in polyphenols and flavonoids, offers inherent antioxidant, anti-inflammatory, and UV-absorbing properties but lacks mechanical stability for topical applications. This study addresses this gap by integrating FE with bovine serum albumin (BSA) to develop an amyloidogenic hydrogel in film and soft hydrogel forms. Leveraging BSA’s heat-induced fibrillation, the composite hydrogel combines FE’s bioactive benefits with a tunable protein network, eliminating the need for chemical crosslinkers. Comprehensive characterization confirmed the hydrogel’s shear-thinning behaviour, viscoelastic stability, and enhanced UV shielding compared to BSA alone. Biological evaluations highlighted its biocompatibility, significant protection against UVB-induced oxidative stress, and promotion of tissue repair through anti-inflammatory and antioxidant mechanisms. The film formulation demonstrated superior mechanical robustness and prolonged adhesion, making it ideal for long-term photoprotection. This work underscores the potential of natural, protein-polyphenol synergies in creating multifunctional hydrogels for skincare, bridging UV protection with tissue regeneration.
{"title":"Nature-inspired dermal barrier: Tunable biofunctional BSA-fenugreek films and spreadable soft hydrogel for skin photoprotection and healing","authors":"Shikha Tripathi , Nitesh Kumar Mishra , Sakshi Agarwal , Anjali Upadhyay , Shilpi Chaudhary , Sudip Mukherjee , Sanjeev Kumar Yadav , Avanish Singh Parmar","doi":"10.1016/j.colsurfb.2025.115358","DOIUrl":"10.1016/j.colsurfb.2025.115358","url":null,"abstract":"<div><div>The increasing prevalence of UV-induced skin damage and the limitations of synthetic sunscreens, such as toxicity, environmental harm, and inadequate multifunctionality, have driven demand for natural, biocompatible alternatives. Fenugreek (<em>Trigonella foenum-graecum</em>) seed extract (FE), rich in polyphenols and flavonoids, offers inherent antioxidant, anti-inflammatory, and UV-absorbing properties but lacks mechanical stability for topical applications. This study addresses this gap by integrating FE with bovine serum albumin (BSA) to develop an amyloidogenic hydrogel in film and soft hydrogel forms. Leveraging BSA’s heat-induced fibrillation, the composite hydrogel combines FE’s bioactive benefits with a tunable protein network, eliminating the need for chemical crosslinkers. Comprehensive characterization confirmed the hydrogel’s shear-thinning behaviour, viscoelastic stability, and enhanced UV shielding compared to BSA alone. Biological evaluations highlighted its biocompatibility, significant protection against UVB-induced oxidative stress, and promotion of tissue repair through anti-inflammatory and antioxidant mechanisms. The film formulation demonstrated superior mechanical robustness and prolonged adhesion, making it ideal for long-term photoprotection. This work underscores the potential of natural, protein-polyphenol synergies in creating multifunctional hydrogels for skincare, bridging UV protection with tissue regeneration.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"259 ","pages":"Article 115358"},"PeriodicalIF":5.6,"publicationDate":"2025-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145733470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-09DOI: 10.1016/j.colsurfb.2025.115360
Qiaozheng Wang , Xiaofei Liu , Yongguang Yu , Xianwei Meng , Hongshan Zhong
Microwave ablation (MWA) therapy has gained prominence as an effective yet minimally invasive hyperthermic method for the treatment of hepatocellular carcinoma (HCC). The upregulation of programmed death ligand 1 (PD-L1) and insufficient antitumor immune response induced by thermal injury, however, substantially limit the long-term efficacy of MWA. Suppression of PD-L1 combined with reactive oxygen species (ROS)-enhanced immunogenic cell death (ICD) mediated antitumor immunity is anticipated to improve the MWA prognosis. In this study, a Sunitinib (SUN)-loaded Fe-Cu MOF was designed to prevent tumor recurrence following MWA. The released Sunitinib effectively inhibits PD-L1 expression, which is upregulated by MWA-induced hyperthermic injury. Moreover, the MWA-enhanced dynamic sensitization of Fe-Cu MOF increases ROS production, thereby promoting stronger ICD and enhancing the SUN anti-PD-L1 effect. The presence of Cu and Fe with T1/T2 MR imaging properties enables real-time image-guided monitoring of MWA. Notably, the Fe-Cu MOF@PEG@SUN nanocomposites effectively counteracted MWA-induced PD-L1 upregulation and amplified the extent of ICD post-MWA, thereby enhancing the SUN-mediated anti-PD-L1 immune response and promoting antitumor immunity. Hence, this study offers a promising strategy and theoretical foundation for the integration of diagnostic imaging with MWA-based therapy for HCC.
{"title":"Suppressing hyperthermia-induced up-regulated PD-L1 with a Sunitinib loaded Fe-Cu MOF: Strengthening immunogenic cell death to sensitize anti-PD-L1 effect following microwave ablation of hepatocellular carcinoma","authors":"Qiaozheng Wang , Xiaofei Liu , Yongguang Yu , Xianwei Meng , Hongshan Zhong","doi":"10.1016/j.colsurfb.2025.115360","DOIUrl":"10.1016/j.colsurfb.2025.115360","url":null,"abstract":"<div><div>Microwave ablation (MWA) therapy has gained prominence as an effective yet minimally invasive hyperthermic method for the treatment of hepatocellular carcinoma (HCC). The upregulation of programmed death ligand 1 (PD-L1) and insufficient antitumor immune response induced by thermal injury, however, substantially limit the long-term efficacy of MWA. Suppression of PD-L1 combined with reactive oxygen species (ROS)-enhanced immunogenic cell death (ICD) mediated antitumor immunity is anticipated to improve the MWA prognosis. In this study, a Sunitinib (SUN)-loaded Fe-Cu MOF was designed to prevent tumor recurrence following MWA. The released Sunitinib effectively inhibits PD-L1 expression, which is upregulated by MWA-induced hyperthermic injury. Moreover, the MWA-enhanced dynamic sensitization of Fe-Cu MOF increases ROS production, thereby promoting stronger ICD and enhancing the SUN anti-PD-L1 effect. The presence of Cu and Fe with T<sub>1</sub>/T<sub>2</sub> MR imaging properties enables real-time image-guided monitoring of MWA. Notably, the Fe-Cu MOF@PEG@SUN nanocomposites effectively counteracted MWA-induced PD-L1 upregulation and amplified the extent of ICD post-MWA, thereby enhancing the SUN-mediated anti-PD-L1 immune response and promoting antitumor immunity. Hence, this study offers a promising strategy and theoretical foundation for the integration of diagnostic imaging with MWA-based therapy for HCC.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"259 ","pages":"Article 115360"},"PeriodicalIF":5.6,"publicationDate":"2025-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145733468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-09DOI: 10.1016/j.colsurfb.2025.115362
Ziyan Li , Fengguang Ma , Yang You , Mengjia Jin , Yunqi Li , Yuan Liu , Hui Zhao , Bing Hu
To address the critical challenge of uncontrolled hemorrhage in surgical and traumatic scenarios, the hemostatic materials with excellent bio-adhesion capabilities, antibacterial properties, antioxidant activities, biocompatibility and biodegradability have gained increasing attention. In this study, homogeneous (-)-Epigallocatechin-3-gallate (EGCG) incorporated mung bean protein fibril-chitosan composite films for hemostasis were prepared under thermal acidic treatment. The addition of EGCG enhanced the mechanical and swelling properties of the protein-chitosan composite films, with the tensile strength reaching up to 17 MPa, the elongation at break approaching 60 % and the swelling rate reaching to 200 %, respectively. The small-angle X-ray scattering (SAXS) and scanning electron microscopy (SEM) analyses revealed the formation of enlarged aggregates formed by multiple globular aggregates induced by EGCG, resulting in rougher surfaces in microscale in the films. When incorporated with EGCG, the films showed excellent adhesion, superior broad-spectrum antibacterial properties and antioxidant activities, which achieved tight adhesion to multiple wet tissues within a few seconds and reduced the CFUs of E. coli, S. aureus, P. aeruginosa and A. baumannii below the limit of detection (50 CFU/mL) within 3 h. In addition, no cytotoxicity and hemolysis of the extract solutions derived from the films were observed, indicating their favorable biocompatibility and biosafety. Furthermore, the composite films reduced total blood loss by over 80 % and decreased hemostatic time by nearly 90 % in a mouse liver hemorrhage model. The composite films were completely degraded both in vivo and in the natural environment and the subcutaneous implantation of the films did not induce inflammatory response in surrounding tissues, suggesting the potential as a safe, efficient, and environmentally friendly hemostatic material.
{"title":"Protein fibril-chitosan composite films incorporated with epigallocatechin gallate for emergency hemostasis","authors":"Ziyan Li , Fengguang Ma , Yang You , Mengjia Jin , Yunqi Li , Yuan Liu , Hui Zhao , Bing Hu","doi":"10.1016/j.colsurfb.2025.115362","DOIUrl":"10.1016/j.colsurfb.2025.115362","url":null,"abstract":"<div><div>To address the critical challenge of uncontrolled hemorrhage in surgical and traumatic scenarios, the hemostatic materials with excellent bio-adhesion capabilities, antibacterial properties, antioxidant activities, biocompatibility and biodegradability have gained increasing attention. In this study, homogeneous (-)-Epigallocatechin-3-gallate (EGCG) incorporated mung bean protein fibril-chitosan composite films for hemostasis were prepared under thermal acidic treatment. The addition of EGCG enhanced the mechanical and swelling properties of the protein-chitosan composite films, with the tensile strength reaching up to 17 MPa, the elongation at break approaching 60 % and the swelling rate reaching to 200 %, respectively. The small-angle X-ray scattering (SAXS) and scanning electron microscopy (SEM) analyses revealed the formation of enlarged aggregates formed by multiple globular aggregates induced by EGCG, resulting in rougher surfaces in microscale in the films. When incorporated with EGCG, the films showed excellent adhesion, superior broad-spectrum antibacterial properties and antioxidant activities, which achieved tight adhesion to multiple wet tissues within a few seconds and reduced the CFUs of <em>E. coli</em>, <em>S. aureus</em>, <em>P. aeruginosa</em> and <em>A. baumannii</em> below the limit of detection (50 CFU/mL) within 3 h. In addition, no cytotoxicity and hemolysis of the extract solutions derived from the films were observed, indicating their favorable biocompatibility and biosafety. Furthermore, the composite films reduced total blood loss by over 80 % and decreased hemostatic time by nearly 90 % in a mouse liver hemorrhage model. The composite films were completely degraded both <em>in vivo</em> and in the natural environment and the subcutaneous implantation of the films did not induce inflammatory response in surrounding tissues, suggesting the potential as a safe, efficient, and environmentally friendly hemostatic material.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"259 ","pages":"Article 115362"},"PeriodicalIF":5.6,"publicationDate":"2025-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145772981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Understanding biomolecular coronas that spontaneously occur around liposomes in biological fluids is critical as both the lipid and protein coronas influence liposome behavior in biological systems. Herein, PEGylated liposomes were pre-incubated at varying plasma concentrations followed by in vitro dynamic simulation incubation. It was found that increasing plasma concentration from 10 % to 300 % resulted in reduced liposomes uptake by immune cells. Most plasma-derived lipids were found to be retained in the coronas but with altered abundances. Lipids such as CE 18:1 and PC 16:0/18:2 exhibited plasma pre-incubation concentration-dependent changes. Changes in the plasma concentration resulted in the emergence of unique proteins in the final protein corona. Using cross-linking mass spectrometry with two crosslinkers and time-limited proteolysis-mass spectrometry, the associations between plasma pre-incubation concentrations and the topological network of the protein corona, the molecular orientation of proteins including alpha-actinin-4, apolipoprotein A1, etc., on the liposome surface, as well as domain proximity, were identified based on peptide-level structural resolution. Among the detected protein complexes with inter-protein crosslinks, over 85 % were not documented in the BioGRID and STRING databases, and more than 90 % of these complexes failed to align with the predictive models generated by AlphaFold Multimer. These results underscore the characteristic weak interactions between proteins within the protein corona on the surface of PEGylated liposomes. The biomolecular corona on the surface of PEGylated liposomes retains a historical imprint of the microenvironment it has experienced, ultimately shaping the authentic biological identity of the liposomes.
{"title":"Plasma pre-incubation: Concentration-dependent regulation of the biomolecular corona on PEGylated liposome and cellular uptake","authors":"Ziyi Zheng, Zhihua Shen, Chaohua Feng, Guo Xie, Wenli Liu, Ziqiang Pan, Guiliang Tan","doi":"10.1016/j.colsurfb.2025.115355","DOIUrl":"10.1016/j.colsurfb.2025.115355","url":null,"abstract":"<div><div>Understanding biomolecular coronas that spontaneously occur around liposomes in biological fluids is critical as both the lipid and protein coronas influence liposome behavior in biological systems. Herein, PEGylated liposomes were pre-incubated at varying plasma concentrations followed by in <em>vitro</em> dynamic simulation incubation. It was found that increasing plasma concentration from 10 % to 300 % resulted in reduced liposomes uptake by immune cells. Most plasma-derived lipids were found to be retained in the coronas but with altered abundances. Lipids such as CE 18:1 and PC 16:0/18:2 exhibited plasma pre-incubation concentration-dependent changes. Changes in the plasma concentration resulted in the emergence of unique proteins in the final protein corona. Using cross-linking mass spectrometry with two crosslinkers and time-limited proteolysis-mass spectrometry, the associations between plasma pre-incubation concentrations and the topological network of the protein corona, the molecular orientation of proteins including alpha-actinin-4, apolipoprotein A1, etc., on the liposome surface, as well as domain proximity, were identified based on peptide-level structural resolution. Among the detected protein complexes with inter-protein crosslinks, over 85 % were not documented in the BioGRID and STRING databases, and more than 90 % of these complexes failed to align with the predictive models generated by AlphaFold Multimer. These results underscore the characteristic weak interactions between proteins within the protein corona on the surface of PEGylated liposomes. The biomolecular corona on the surface of PEGylated liposomes retains a historical imprint of the microenvironment it has experienced, ultimately shaping the authentic biological identity of the liposomes.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"259 ","pages":"Article 115355"},"PeriodicalIF":5.6,"publicationDate":"2025-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145733384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-08DOI: 10.1016/j.colsurfb.2025.115353
Ke Zhan, Yuting Zhao, Haiying Li, Shan Jiang
The persistent threat of bacterial infections, which contribute to significant global morbidity and mortality, highlights the urgent demand for developing advanced antimicrobial materials. Herein, octopod-shaped silver-gold alloy nanostructures (Ag-Au octopods) were synthesized through a controlled deposition of gold onto silver octopods. Ag-Au octopods can convert near-infrared (NIR) light into thermal energy with photothermal conversion efficiency of 41.5 %. They also exhibited peroxidase-like nanozyme activity, catalysing the decomposition of trace amounts of H2O2 to produce significant levels of reactive oxygen species (ROS). Notably, this catalytic activity was further enhanced upon exposure to NIR light. During in vitro antibacterial tests, a dose of 32 µg mL−1 of Ag–Au octopods, together with H2O2 and 10 min of NIR irradiation, achieved a bactericidal rate greater than 99.0 %. Mechanistic investigations revealed that the antimicrobial effect arises from the disruption of bacterial membrane integrity, leakage of intracellular nucleic acids, a rise in intracellular ROS, and subsequent oxidative stress. The results present an effective and synergistic antibacterial strategy using the combined photothermal and nanozyme activities, offering a promising direction for next-generation antibacterial applications.
细菌感染的持续威胁导致了全球显著的发病率和死亡率，这凸显了开发先进抗菌材料的迫切需求。本文通过控制金沉积在银章鱼体上，合成了章鱼形银金合金纳米结构（Ag-Au octopods）。Ag-Au章鱼可以将近红外（NIR）光转化为热能，光热转换效率为41.5 %。它们还表现出类似过氧化物酶的纳米酶活性，催化微量H2O2的分解，产生大量的活性氧（ROS）。值得注意的是，这种催化活性在近红外光照射下进一步增强。在体外抗菌试验中，32 µg mL - 1 Ag-Au章鱼体，H2O2和10 min的近红外照射，杀菌率大于99.0 %。机制研究表明，抗菌作用源于细菌膜完整性的破坏、细胞内核酸的泄漏、细胞内ROS的增加以及随后的氧化应激。结果表明，利用光热和纳米酶的联合活性，可以有效地协同抗菌，为下一代抗菌应用提供了一个有前途的方向。
{"title":"Octopod-shaped silver–gold alloy for NIR-driven synergistic photothermal and catalytic bacterial ablation","authors":"Ke Zhan, Yuting Zhao, Haiying Li, Shan Jiang","doi":"10.1016/j.colsurfb.2025.115353","DOIUrl":"10.1016/j.colsurfb.2025.115353","url":null,"abstract":"<div><div>The persistent threat of bacterial infections, which contribute to significant global morbidity and mortality, highlights the urgent demand for developing advanced antimicrobial materials. Herein, octopod-shaped silver-gold alloy nanostructures (Ag-Au octopods) were synthesized through a controlled deposition of gold onto silver octopods. Ag-Au octopods can convert near-infrared (NIR) light into thermal energy with photothermal conversion efficiency of 41.5 %. They also exhibited peroxidase-like nanozyme activity, catalysing the decomposition of trace amounts of H<sub>2</sub>O<sub>2</sub> to produce significant levels of reactive oxygen species (ROS). Notably, this catalytic activity was further enhanced upon exposure to NIR light. During in vitro antibacterial tests, a dose of 32 µg mL<sup>−1</sup> of Ag–Au octopods, together with H<sub>2</sub>O<sub>2</sub> and 10 min of NIR irradiation, achieved a bactericidal rate greater than 99.0 %. Mechanistic investigations revealed that the antimicrobial effect arises from the disruption of bacterial membrane integrity, leakage of intracellular nucleic acids, a rise in intracellular ROS, and subsequent oxidative stress. The results present an effective and synergistic antibacterial strategy using the combined photothermal and nanozyme activities, offering a promising direction for next-generation antibacterial applications.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"259 ","pages":"Article 115353"},"PeriodicalIF":5.6,"publicationDate":"2025-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145733469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
扫码关注我们
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号