Colloids and Surfaces B: Biointerfaces最新文献_第9页

The cinnamaldehyde-thiosemicarbazone-zinc (II) complex induces apoptosis in CAL-27 cells 肉桂醛-硫代氨基脲-锌（II）复合物诱导CAL-27细胞凋亡

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2026-04-01 Epub Date: 2025-12-16 DOI: 10.1016/j.colsurfb.2025.115374

Chen-yan Li , Shuohua Xie , Min Deng , Ganrong Huang , Yanqiang Huang , Shufang Li

Objectives

Oral squamous cell carcinoma (OSCC) is a major malignancy affecting the oral, jaw, and facial regions. In this study, we synthesized a cinnamaldehyde-thiosemicarbazone-zinc (II) complex (CTZn) to inhibit OSCC cell proliferation.

Methods

We investigated the inhibitory effects of CTZn on OSCC using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, cell colony formation assays, wound-healing/scratch assays and transwell invasion assays. Flow cytometry (FCM) was performed to explore the CTZn's impact on apoptosis and cell-cycle progression. Through in vivo experiments, its antitumor activity activity was evaluated in OSCC xenograft mouse models and orthotopic tumor model mice. The mechanisms of CTZn were explored by detecting targeted metabolites, and performing drug affinity responsive target stability (DARTS) experiments, molecular docking analyses and Western blot assays. Levels of reactive oxygen species (ROS), oxidized and reduced nicotinamide adenine dinucleotide phosphate (NADP⁺/NADPH), glutathione (GSH), and 6-phosphogluconate dehydrogenase (PGD) protein in CAL-27 cells were also determined.

Results

These findings demonstrated that CTZn inhibited CAL-27 cell growth in vitro in a time- and concentration-dependent manner, with an IC₅₀ value of 1.642–2.223μmol/L, and CTZn also exerted anti-tumor activity in vivo. CTZn also inhibited PGD messenger RNA (mRNA), and protein expression, reduced NADP+ /NADPH and GSH levels, and significantly increased ROS levels, thereby inducing oxidative stress.

Conclusion

CTZn impairs mitochondrial function, decreases ATP, levels, and induces G₂-phase arrest and apoptosis in CAL-27 cells. Therefore, it is an ideal drug for treating OSCC.

目的口腔鳞状细胞癌（OSCC）是一种影响口腔、颌部和面部的主要恶性肿瘤。在本研究中，我们合成了肉桂醛-硫代氨基脲-锌（II）配合物（CTZn）来抑制OSCC细胞的增殖。方法采用3-（4,5-二甲基噻唑-2-基）-2,5-二苯基溴化四唑（MTT）实验、细胞集落形成实验、伤口愈合/划痕实验和跨井侵袭实验研究CTZn对OSCC的抑制作用。流式细胞术（FCM）探讨CTZn对细胞凋亡和细胞周期进程的影响。通过体内实验，评价其在OSCC异种移植小鼠模型和原位肿瘤模型小鼠中的抗肿瘤活性。通过检测靶向代谢物，进行药物亲和力响应性靶稳定性（dart）实验、分子对接分析和Western blot分析，探索CTZn的作用机制。还检测了CAL-27细胞中活性氧（ROS）、氧化和还原的烟酰胺腺嘌呤二核苷酸磷酸（NADP + /NADPH）、谷胱甘肽（GSH）和6-磷酸葡萄糖酸脱氢酶（PGD）蛋白的水平。结果CTZn体外抑制CAL-27细胞生长呈时间和浓度依赖性，IC50值为1.642 ~ 2.223μmol/L，体内也具有抗肿瘤活性。CTZn还能抑制PGD信使RNA （mRNA）和蛋白表达，降低NADP+ /NADPH和GSH水平，显著增加ROS水平，从而诱导氧化应激。结论ctzn可损伤CAL-27细胞线粒体功能，降低ATP水平，诱导G₂相阻滞和细胞凋亡。因此，它是治疗OSCC的理想药物。

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引用次数: 0

A novel macrophage membrane-camouflaged ultra-small copper sulfide and photothermal-responsive nitric oxide donor nanocomposites for enhanced synergistic antitumor therapy 一种新型巨噬细胞膜伪装的超小硫化铜和光热响应型一氧化氮供体纳米复合材料，用于增强协同抗肿瘤治疗

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2026-04-01 Epub Date: 2025-12-15 DOI: 10.1016/j.colsurfb.2025.115373

Haoyang Gong , Xiaonan Li , Xinru Feng , Dongjing Wang , Xinyi Zhang , Kai Wang , Yanchen Liu , Lu Xu , Xueyan Zhou , Yanzhuo Zhang

Ultra-small copper sulfide (CuS) nanoparticles (NPs) possess exceptional photothermal performance, Fenton-like reaction activities, and multimodal imaging characteristics, while sodium nitroprusside serves as a clinical nitric oxide (NO) donor. A judicious combination of these two agents may facilitate the development of a novel multimodal synergistic antitumor strategy. In this context, we developed macrophage membrane-camouflaged and folic acid-conjugated nanocomposites (MF-SPC) that demonstrate dual responsiveness to near-infrared (NIR) laser and pH, aiming to enhance multimodal synergistic antitumor therapy. Within these biomimetic nanocomposites, sodium nitroprusside-doped Prussian blue NPs serve as the cores for inducing photothermal-responsive NO donation, while polydopamine layer embedded with CuS dots (sub-5 nm) act as the shells. This configuration not only enhances photothermal effects but also promotes Fenton-like reaction activity, glutathione depletion capabilities, and peroxidase activity. Furthermore, the dopamine groups on the shell surface facilitate folic acid modification, drug loading, and macrophage membrane camouflage. These functionalities collectively improve tumor-targeting abilities, promote immune evasion, and enhance tumor uptake. The doxorubicin-loaded MF-SPC (MDF-SPC) exhibited favorable dispersibility, stability, and pH-responsive sustained release properties. Both in vivo fluorescence imaging and NIR thermal imaging show that the MDF-SPC possessed active tumor-targeting capability. Thus, the MDF-SPC demonstrated high antitumor activity and biosafety when subjected to NIR laser irradiation in 4T1 tumor-bearing mice. Overall, the active tumor-targeting and retention capabilities, along with the rapid temperature elevations produced by PTT, allow the MDF-SPC to precisely and swiftly amplify chemodynamic therapy, gas therapy, and chemotherapy, providing a novel multimodal nanoplatform to promote antitumor therapy.

超小硫化铜纳米颗粒（cu）具有优异的光热性能、芬顿样反应活性和多模态成像特性，而硝普钠可作为临床一氧化氮（NO）供体。这两种药物的明智组合可能促进一种新的多模式协同抗肿瘤策略的发展。在此背景下，我们开发了巨噬细胞膜伪装和叶酸共轭纳米复合材料（MF-SPC），其对近红外（NIR）激光和pH值具有双重响应性，旨在增强多模态协同抗肿瘤治疗。在这些仿生纳米复合材料中，硝普钠掺杂的普鲁士蓝NPs作为诱导光热响应性NO捐赠的核心，而嵌入cu点（亚5 nm）的聚多巴胺层作为壳层。这种结构不仅增强了光热效应，而且促进了芬顿样反应活性、谷胱甘肽耗竭能力和过氧化物酶活性。此外，壳表面的多巴胺基团促进叶酸修饰，药物装载和巨噬细胞膜伪装。这些功能共同提高肿瘤靶向能力，促进免疫逃避，增强肿瘤摄取。负载多柔比星的MF-SPC （MDF-SPC）具有良好的分散性、稳定性和ph响应缓释性能。体内荧光成像和近红外热成像均显示MDF-SPC具有活跃的肿瘤靶向能力。因此，MDF-SPC在4T1荷瘤小鼠近红外激光照射下表现出较高的抗肿瘤活性和生物安全性。总的来说，活跃的肿瘤靶向和保留能力，以及PTT产生的快速温度升高，使MDF-SPC能够精确、快速地放大化学动力学治疗、气体治疗和化疗，为促进抗肿瘤治疗提供了一种新的多模态纳米平台。

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引用次数: 0

Vibrio cholerae adhesin-derived peptides mediate strong pull-off forces in aqueous media with high ionic strength 霍乱弧菌黏附素衍生肽在高离子强度的水介质中介导强牵引力

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2026-04-01 Epub Date: 2025-12-24 DOI: 10.1016/j.colsurfb.2025.115390

Syeda Tajin Ahmed , Sixin Zhai , Xin Huang , Sarvagya Sulaja , Adekunle Adewole , Alisa Ioffe , Andrea D. Merg , Jing Yan , Roberto C. Andresen Eguiluz

In this study, the pull-off forces of adsorbed films of four Bap1-inspired peptides in various solvents were investigated on negatively charged mica substrates using the surface forces apparatus (SFA), complemented with dynamic light scattering (DLS) for characterizing the aggregation behavior of peptides in solution. Bap1-inspired peptides consisted of the 57 amino acid wild-type sequence (WT); a scrambled version of the WT used to investigate the impact of the primary amino acid sequence in pull-off forces (Scr); a ten amino acid sequence rich in hydrophobic content (CP) of the WT sequence, and an eight amino acid sequence (Sh1) that corresponds to the pseudo-repeating sequence in the 57 AA. SFA results showed remarkable pull-off forces for CP, particularly in the presence of salts: measured pull-off forces were 26.0 ± 7.0 mN/m for no dwell-time and up to 42.0 ± 8.8 mN/m when surfaces were left in contact for 30 min. Sh1 did not bridge mica surfaces, while large aggregates clouded SFA measurements of Scr. DLS observations indicated that salts favored large peptide aggregation for all constructs (D_H > 1 µm), as compared to milliQ (D_H ≈ 100–500 nm) water and DMSO (D_H ≈ 5 nm), resulting in heterogeneous peptide film thicknesses.

在本研究中，利用表面力仪（SFA）研究了四种bap1激发肽在不同溶剂中的吸附膜在带负电荷的云母基质上的拉脱力，并利用动态光散射（DLS）表征了肽在溶液中的聚集行为。bap1激发肽由57个氨基酸的野生型序列（WT）组成；用于研究初级氨基酸序列对拉脱力（Scr）影响的WT的打乱版本；一个富含WT序列疏水含量（CP）的10个氨基酸序列和一个与57aa中的伪重复序列对应的8个氨基酸序列（Sh1）。SFA结果显示CP的剥离力显著，特别是在有盐存在的情况下：未停留时间的剥离力为26.0 ±7.0 mN/m，当表面接触30 min时，剥离力高达42.0 ±8.8 mN/m。Sh1不能桥接云母表面，而大聚集体遮蔽了Scr的SFA测量。DLS观察表明，与milliQ （DH≈100-500 nm）水和DMSO （DH≈5 nm）相比，盐有利于所有结构的大肽聚集（DH > 1 µm），导致肽膜厚度不均。

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引用次数: 0

Biointerfacial mechanical responses of supported lipid membranes to Gramicidin D revealed by QCM-D QCM-D研究支持脂质膜对Gramicidin D的生物界面力学响应

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2026-04-01 Epub Date: 2025-12-26 DOI: 10.1016/j.colsurfb.2025.115399

Şebnem Seherler, Abdulhalim Kılıç

Gramicidin D (GrD) is a well-established model ion channel–forming peptide whose mechanical interactions with supported lipid membrane architectures remain less well characterized. Here, we combine overtone-resolved Quartz Crystal Microbalance with Dissipation (QCM-D), fluorescence microscopy, and solution-phase assays to examine how GrD perturbs supported lipid bilayers (SLBs) and supported vesicular layers (SVLs) formed from neutral DOPC and anionic DOPC:DOPS (92:08). On neutral SLBs at low peptide levels, GrD induces mild outer-leaflet softening consistent with β^6.3 channel formation, producing subtle acoustic signatures that evolve with slow kinetics. In contrast, neutral SVLs exhibit immediate hydration and thickening of the vesicle shell, reflected by large dissipation increases and overtone-dependent frequency shifts. Increasing peptide levels drive both platforms into a mechanically disruptive regime characterized by distinct overtone divergence and the formation of highly dissipative peptide–lipid assemblies, including rapid disruptive remodeling on SLBs and collapse of peptide-rich outer shells on SVLs. Membrane charge further modulates these responses. Anionic SLBs and SVLs confine GrD to shallow, leaflet-restricted perturbations near the headgroup region, yielding smaller overall mass changes but strong, overtone-specific viscoelastic heterogeneity. Solution-phase turbidity and proton permeability assay are consistent with functional channel activity dominating at lower peptide levels, whereas higher peptide levels align with non-conducting, mechanically disruptive interaction states. Together, these results show that GrD exhibits distinct, architecture-dependent mechanical interaction modes governed by peptide density, membrane geometry, and surface charge, and demonstrate how QCM-D measurements on complementary SLB and SVL platforms resolve nanoscale hydration and mechanical response modes that remain inaccessible to conventional ensemble assays.

Gramicidin D （GrD）是一种建立良好的离子通道形成模型肽，其与支持的脂质膜结构的机械相互作用尚未得到很好的表征。在这里，我们结合了泛音分辨石英晶体微平衡与耗散（QCM-D），荧光显微镜和液相分析来研究GrD如何干扰由中性DOPC和阴离子DOPC:DOPS形成的脂质双层（slb）和支持囊泡层（svl）。在低肽水平的中性slb上，GrD诱导与β6.3通道形成一致的轻度外小叶软化，产生缓慢动力学演变的微妙声学特征。相比之下，中性svl表现出立即水化和囊泡壳增厚，反映在大的耗散增加和泛音相关的频移上。不断增加的肽水平驱动两个平台进入机械破坏状态，其特征是明显的泛音发散和高度耗散的肽脂组装的形成，包括slb上的快速破坏性重塑和sll上富含肽的外壳的崩溃。膜电荷进一步调节这些反应。阴离子slb和svl将GrD限制在头群附近的浅的、叶片受限的扰动中，产生较小的总体质量变化，但产生强烈的泛音特异性粘弹性非均质性。溶液浊度和质子渗透性分析与功能通道活性在低肽水平下占主导地位一致，而高肽水平与非导电、机械破坏性相互作用状态一致。总之，这些结果表明，GrD表现出由肽密度、膜几何形状和表面电荷控制的独特的结构依赖的机械相互作用模式，并展示了QCM-D在互补SLB和SVL平台上的测量如何解决纳米尺度的水化和机械响应模式，这些模式仍然无法通过传统的系综分析。

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引用次数: 0

A user-friendly multifunctional hydrogel spray with adjustable mechanical properties for hemostasis and infected wound healing 一种用户友好的多功能水凝胶喷雾剂，具有可调节的机械性能，用于止血和感染伤口愈合。

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2026-04-01 Epub Date: 2025-12-17 DOI: 10.1016/j.colsurfb.2025.115378

Mengyao Gao , Zongliang Wang , Zhaohui Tang , Peibiao Zhang

Traumatic massive hemorrhage is a critical problem in the global emergency medicine field. Traditional hemostatic materials such as gauze and sponge have problems such as poor adhesion to irregular wounds and low adhesion strength. Existing hemostatic hydrogels also face bottlenecks such as insufficient active hemostatic efficiency, challenges in balancing interface adhesion and flexibility, and a lack of functional synergy, making it difficult to meet clinical needs. To address these challenges, this study develops a self-healing hydrogel spray that can form in situ for efficient hemostasis by simply mixing oxidized dextran (ODex) with polyethyleneimine (PEI) through a dynamic Schiff base reaction. Benefiting from the 'aldehyde-amino' active crosslinking mechanism, this hydrogel spray exhibits rapid gelation, triggering crosslinking within 15 s, with an adhesion strength exceeding 10 kPa, while also possessing good self-healing properties to adapt to dynamic and irregular wound. The electrostatic physical antibacterial properties of polyethyleneimine effectively suppress both Gram-positive and Gram-negative bacteria, avoiding the risk of drug resistance. By adjusting the oxidation degree of dextran, the hemostatic-adhesion synergy of the material has been further optimized, and the hydrogel preparation process is simple, cost-effective, user-friendly and can be stored at room temperature, with good biocompatibility. Animal experiments indicate that this hydrogel spray can quickly cover and adhere to the wound, effectively stopping bleeding, while reducing the risk of infection and promoting wound healing, providing innovative strategies and technical support for the development of a new generation of clinically available traumatic hemostatic materials.

外伤性大出血是目前国际急救医学领域的热点问题。传统的止血材料如纱布、海绵等存在着对不规则伤口黏附性差、黏附强度低等问题。现有的止血水凝胶也存在活性止血效率不足、界面粘连性和柔韧性难以平衡、缺乏功能协同等瓶颈，难以满足临床需求。为了解决这些挑战，本研究开发了一种自我修复的水凝胶喷雾，通过动态希夫碱反应将氧化右旋糖酐（ODex）与聚乙烯亚胺（PEI）混合，即可原位形成有效止血。得益于“醛-氨基”活性交联机制，该水凝胶喷雾具有快速凝胶化，在15 s内触发交联，粘附强度超过10 kPa，同时具有良好的自愈性能，适应动态和不规则伤口。聚乙烯亚胺的静电物理抗菌特性能有效抑制革兰氏阳性菌和革兰氏阴性菌，避免耐药风险。通过调节右旋糖酐的氧化程度，进一步优化了材料的止血-粘附协同作用，制备水凝胶工艺简单、性价比高、用户友好且可常温保存，具有良好的生物相容性。动物实验表明，该水凝胶喷雾剂能快速覆盖并粘附创面，有效止血，同时降低感染风险，促进创面愈合，为开发新一代临床可用的创伤止血材料提供创新策略和技术支持。

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引用次数: 0

Hydrogel composite nanomaterials for diabetic wound treatment 用于糖尿病伤口治疗的水凝胶复合纳米材料。

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2026-04-01 Epub Date: 2025-12-23 DOI: 10.1016/j.colsurfb.2025.115392

Nuonan Li , Xianjun Luo , Hongwen Zhu , Guowang Shen , Meifang Li , Chunxia Zhou , Ning Liang , Puhua Zhang , Xiaoyong Zhu

Diabetes mellitus is slowly becoming more common over the past few years. One of the most disabling effects of diabetes is chronic skin ulcers. This is because certain materials have unique physicochemical properties that make them very useful for treating diabetic wounds. The three-dimensional network structure of hydrogels allows them to absorb large amounts of water. Their ability to bind water better, hold drugs effectively, and release drugs continuously is superior. Nanomaterials, particularly helpful in preventing bacterial infections and diabetic wounds caused by oxidative stress, are defined by at least one dimension measuring under 100 nanometers. Their unique qualities include antimicrobial action, facilitation of cell migration and proliferation, drug delivery systems, antioxidant and anti-inflammatory properties. This review delineates the properties and classifications of hydrogels, emphasizing their benefits as wound dressings. It then discusses the physicochemical properties of nanomaterials and their applications in wound healing. The main focus is on the basics of hydrogel-nanomaterial composites, their preparation techniques, and the synergistic benefits of their combination as wound dressings. The utilization of hydrogel nanocomposites in diabetic wound treatment is elucidated, and prospective research avenues are suggested to offer novel insights for clinical management.

在过去的几年里，糖尿病逐渐变得越来越普遍。糖尿病最严重的影响之一是慢性皮肤溃疡。这是因为某些材料具有独特的物理化学特性，这使得它们对治疗糖尿病伤口非常有用。水凝胶的三维网状结构使它们能够吸收大量的水。它们结合水的能力更好，有效地保持药物，并持续释放药物。纳米材料，尤其有助于防止细菌感染和氧化应激引起的糖尿病伤口，被定义为至少有一个尺寸小于100纳米。它们独特的特性包括抗菌作用、促进细胞迁移和增殖、药物传递系统、抗氧化和抗炎特性。本文综述了水凝胶的性质和分类，强调了它们作为伤口敷料的好处。然后讨论了纳米材料的物理化学性质及其在伤口愈合中的应用。主要重点是水凝胶-纳米材料复合材料的基础知识，它们的制备技术，以及它们作为伤口敷料的组合的协同效益。阐述了水凝胶纳米复合材料在糖尿病伤口治疗中的应用，并提出了未来的研究方向，为临床治疗提供新的见解。

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引用次数: 0

Pullulan-based polymeric nanosystem for KEAP1 siRNA delivery: Mitigating ROS and promoting wound healing in vitro 基于普鲁兰的KEAP1 siRNA递送聚合物纳米系统：减轻ROS并促进体外伤口愈合。

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2026-04-01 Epub Date: 2025-12-11 DOI: 10.1016/j.colsurfb.2025.115352

Gülşen Bayrak, Dilara Karaguzel, Cagatay Karaaslan, Işık Perçin

Recently, siRNA delivery has shown considerable potential for therapeutic use. While commercial options facilitate efficient delivery, they can be toxic to cells to a certain degree. Therefore, using relatively less toxic and safe delivery vehicles can increase the efficiency of therapy. This study examines the utilization of Pullulan–poly(ethyleneimine) nanoparticles (Pullulan-PEI-NPs) as an effective non-viral delivery system for Kelch-like ECH-associated protein 1 (KEAP1) siRNA, with the aims of mitigating oxidative stress and facilitating wound healing in HaCaT cells by enhancing cellular antioxidant mechanisms. The Pullulan-PEI-NPs were synthesized and characterized with Dynamic Light Scattering, Nanoparticle Tracking Analysis, Fourier Transform Infrared Spectroscopy, and electron microscopy techniques. The polyplexes were formed via electrostatic interaction between Pullulan-PEI-NPs and KEAP1 siRNA, and successful siRNA delivery was confirmed by cellular uptake analysis, mRNA expression studies, and KEAP1 and nuclear factor E2-related factor 2 (NRF2) protein level assessments. Pullulan-PEI-NPs had a silencing efficiency of 72 %, which is very close to the commercial transfection agent Lipofectamine 3000 (80 %). In following studies, KEAP1 siRNA delivery with Pullulan-PEI-NPs mitigates TBHP and H₂O₂ mediated ROS and facilitates wound healing with 98.9 % wound closure in a scratch assay on HaCaT cells. Obtained results highlight the therapeutic relevance of KEAP1 silencing in redox-regulated wound regeneration. This is the first report showing that Pullulan-PEI based cationic polymeric nanoparticles can be used to deliver KEAP1 siRNA and evaluate its role in mitigating ROS and increasing wound healing. These results highlight the promise of Pullulan-PEI-NPs as a safe and adaptable platform for non-viral gene therapy applications.

最近，siRNA递送已显示出相当大的治疗潜力。虽然商业选择有助于高效输送，但它们在一定程度上可能对细胞有毒。因此，使用毒性相对较小且安全的运载工具可以提高治疗效率。本研究探讨了Pullulan-poly（乙亚胺）纳米颗粒（Pullulan-PEI-NPs）作为kelch样ECH-associated protein 1 (KEAP1) siRNA的有效非病毒递送系统的利用，目的是通过增强细胞抗氧化机制来减轻HaCaT细胞的氧化应激和促进伤口愈合。合成了Pullulan-PEI-NPs，并利用动态光散射、纳米颗粒跟踪分析、傅里叶变换红外光谱和电子显微镜技术对其进行了表征。Pullulan-PEI-NPs与KEAP1 siRNA通过静电相互作用形成复合物，并通过细胞摄取分析、mRNA表达研究以及KEAP1和核因子e2相关因子2 （NRF2）蛋白水平评估证实siRNA成功传递。Pullulan-PEI-NPs的沉默效率为72 %，与商业转染剂Lipofectamine 3000（80 %）非常接近。在随后的研究中，KEAP1 siRNA与Pullulan-PEI-NPs一起递送可减轻thbhp和H2O2介导的ROS，并促进伤口愈合，在HaCaT细胞的划痕实验中，伤口愈合率达到98.9 %。获得的结果强调了KEAP1沉默在氧化还原调节的伤口再生中的治疗相关性。这是首次报道基于Pullulan-PEI的阳离子聚合物纳米颗粒可用于递送KEAP1 siRNA，并评估其在减轻ROS和促进伤口愈合中的作用。这些结果突出了Pullulan-PEI-NPs作为一种安全、适应性强的非病毒基因治疗应用平台的前景。

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引用次数: 0

Advances in coagulation peptides: Exploring diverse functions from hemostasis to disease treatment 凝血肽的研究进展：探索从止血到疾病治疗的多种功能

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2026-04-01 Epub Date: 2025-12-23 DOI: 10.1016/j.colsurfb.2025.115383

Wenwen Ma , Yumei Wang , Xin Liu , Wen Li , Wenxue Zhao , Jumeng Di , Hailin Cong , Bing Yu

Coagulation dysfunction and bleeding control remain major challenges in trauma, surgery, and interventional therapy. Millions of patients die from excessive blood loss every year. Traditional hemostatic materials such as gelatin sponge, chitosan, and zeolite have limitations such as a single mechanism of action and poor biological stability. At the same time, clotting peptides have attracted much attention due to their low molecular weight, high biological activity, and precisely designed molecular structure. This paper systematically reviews the latest progress of coagulation peptides as a new hemostatic material, discusses their interaction mechanism with coagulation factors and platelet receptors, and analyzes the structural design strategy. Subsequently, this article critically analyzed the clinical trial data, efficacy comparison, and long-term safety evaluation of clotting peptides in the fields of gastrointestinal endoscopy, high-risk surgery, and systemic hemostasis. Finally, this article summarizes the key clinical translational barriers faced by the study of clotting peptides, including challenges such as long-term biocompatibility, potential thrombosis risks, and in vivo degradation characteristics. This review aims to provide systematic theoretical guidance and objective clinical transformation analysis for the rational design and performance optimization of a new generation of multifunctional coagulation peptide materials.

凝血功能障碍和出血控制仍然是创伤、手术和介入治疗的主要挑战。每年有数百万病人死于失血过多。传统止血材料如明胶海绵、壳聚糖、沸石等存在作用机制单一、生物稳定性差等局限性。同时，凝血肽因其低分子量、高生物活性和精确设计的分子结构而备受关注。本文系统综述了凝血肽作为一种新型止血材料的最新进展，讨论了其与凝血因子和血小板受体的相互作用机制，并分析了其结构设计策略。随后，本文批判性地分析了凝血肽在胃肠内镜、高危手术、全身止血等领域的临床试验数据、疗效比较和长期安全性评价。最后，本文总结了凝血肽研究面临的主要临床转化障碍，包括长期生物相容性、潜在血栓形成风险和体内降解特性等挑战。本文旨在为新一代多功能凝血肽材料的合理设计和性能优化提供系统的理论指导和客观的临床转化分析。

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引用次数: 0

Microrobots/micromotors in cancer therapeutics, advances and applications 微型机器人/微型马达在癌症治疗中的进展和应用。

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2026-04-01 Epub Date: 2025-12-23 DOI: 10.1016/j.colsurfb.2025.115394

Hezhe Huang, Yuqing Miao, Yuhao Li

Cancer remains one of the most challenging diseases to conquer due to its high mortality rate and the lack of effective diagnostic and therapeutic tools. Microrobot technology has brought revolutionary advancements to the field of tumor medicine. By leveraging its micro/nanoscale dimensions and precise propulsion and navigation capabilities, it has enhanced the accuracy and efficiency of cancer diagnosis and treatment. This review provides a detailed exploration of microrobots’ motion from the perspectives of propulsion and navigation. It systematically summarizes its key applications in cancer therapy. Microrobots, which utilize gas, magnetic, light, or ultrasound-based propulsion mechanisms, and are combined with asymmetrical morphologies or biotargeted navigation, can traverse complex biological barriers such as the blood-brain barrier and mucus barrier. They enable precise delivery and controlled release of anticancer drugs. In the realm of diagnosis and imaging, microrobots, by carrying fluorescent probes or contrast agents, have improved the sensitivity of tumor detection and the resolution of deep tissue imaging. Furthermore, microrobots, when coordinated with photothermal therapy, sonodynamic therapy, or immunotherapy, have enhanced tumor eradication effects. They also activate immune responses to inhibit metastatic lesions. Finally, this review provides an outlook on the prospects of microrobots in tumor medicine, proposing potential collaborative pathways with emerging technologies. It highlights their vast potential in improving therapeutic efficacy and reducing side effects.

癌症由于其高死亡率和缺乏有效的诊断和治疗工具，仍然是最具挑战性的疾病之一。微型机器人技术为肿瘤医学领域带来了革命性的进步。利用其微纳米尺度和精确的推进和导航能力，它提高了癌症诊断和治疗的准确性和效率。本文从推进和导航的角度对微型机器人的运动进行了详细的探讨。系统总结了其在肿瘤治疗中的主要应用。微型机器人利用气体、磁、光或超声波推进机制，并结合不对称形态或生物定向导航，可以穿越复杂的生物屏障，如血脑屏障和粘液屏障。它们使抗癌药物的精确输送和控制释放成为可能。在诊断和成像领域，微型机器人通过携带荧光探针或造影剂，提高了肿瘤检测的灵敏度和深部组织成像的分辨率。此外，微型机器人与光热疗法、声动力疗法或免疫疗法配合使用时，具有增强的肿瘤根除效果。它们还能激活免疫反应来抑制转移性病变。最后，本文对微型机器人在肿瘤医学中的应用前景进行了展望，并提出了与新兴技术的潜在合作途径。它突出了它们在提高治疗效果和减少副作用方面的巨大潜力。

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引用次数: 0

Frankincense-loaded lactoferrin-conjugated solid lipid nanoparticles for targeted brain delivery and neuroprotection in a scopolamine-induced Alzheimer’s model 乳香负载的乳铁蛋白结合固体脂质纳米颗粒在东莨菪碱诱导的阿尔茨海默病模型中的靶向脑递送和神经保护

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2026-04-01 Epub Date: 2025-12-13 DOI: 10.1016/j.colsurfb.2025.115370

Farimah Moazzam , Ashrafalsadat Hatamian-Zarmi , Marzie Sabaghian , Khadijeh Khezri , Bahman Ebrahimi Hosseinzadeh , Fariba Khodagholi , Moloud Jalili Shahmansouri , Fatemeh Sadat Rashidi

Central nervous system (CNS) disorders remain a major field with substantial unmet therapeutic needs. This study aimed to address the critical challenge of brain drug delivery by employing solid lipid nanoparticles (SLNs) surface-functionalized with lactoferrin (Lf) ligands, from the transferrin family, to facilitate transport across the blood–brain barrier. Frankincense (F), a natural compound with well-documented neuroprotective properties and minimal adverse effects, was encapsulated within (SLNs) using a microemulsion approach. The optimal formulation resulted in nanoparticles (NPs) with an average size of 103.1 ± 0.9 nm, an encapsulation efficiency of 95.2 ± 0.8 %, a drug loading capacity of 8.6 ± 0.1 %, a polydispersity index (PDI) of 0.3 ± 0.08, and a zeta potential of −29.2 ± 0.5 mV. The optimized NPs showed a sustained drug release profile, and ATR-FTIR spectra confirmed the conjugation of lactoferrin to the nanoparticles. To establish translational relevance, the neuroprotective effectiveness of (F-Lf-SLNs) was investigated using a scopolamine-induced Alzheimer's disease animal model. Behavioral tests, along with biochemical and histological analyses, were conducted. The findings demonstrated that (F-Lf-SLNs) significantly improved the brain delivery of frankincense, highlighting their promise as a neuroprotective strategy for CNS disorders. They also effectively protected neurons compared to the scopolamine-induced group. Overall, these results emphasize the key role of drug delivery systems and ligand-targeting methods in enhancing the therapeutic effectiveness of drugs.

中枢神经系统（CNS）疾病仍然是一个主要的领域，有大量未满足的治疗需求。本研究旨在通过使用固体脂质纳米颗粒（sln）表面功能化乳铁蛋白（Lf）配体（来自转铁蛋白家族）来促进通过血脑屏障的运输，从而解决脑药物递送的关键挑战。乳香(F)是一种天然化合物，具有充分证明的神经保护特性和最小的不良反应，使用微乳液方法包被在（sln）内。最优配方导致纳米颗粒(NPs)平均大小103.1 ±0.9 nm,封装效率95.2 ±0.8 %,药物负荷容量8.6 ±0.1 %,多分散性指数(PDI) 0.3±0.08,和29.2−的电动电势 ±0.5 mV。优化后的纳米颗粒具有持续的药物释放特性，ATR-FTIR光谱证实了乳铁蛋白与纳米颗粒的结合。为了建立翻译相关性，使用东莨菪碱诱导的阿尔茨海默病动物模型研究了（f- lf - sln）的神经保护作用。进行了行为学测试以及生化和组织学分析。研究结果表明，（f- lf - sln）显著改善了乳香的大脑递送，突出了它们作为中枢神经系统疾病的神经保护策略的前景。与东莨菪碱诱导组相比，它们还能有效地保护神经元。总之，这些结果强调了药物传递系统和配体靶向方法在提高药物治疗效果方面的关键作用。

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引用次数: 0