Colloids and Surfaces B: Biointerfaces最新文献

英文中文

Three-dimensional bioactive collagen scaffolds incorporated with titanate nanotubes for tissue regeneration

IF 5.4 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2025-03-20 DOI: 10.1016/j.colsurfb.2025.114638

Petra Reinehr , Leonardo Francisco Diel , Fernando Mendonça Diz , Gabriela de Souza Balbinot , Wesley Formentin Monteiro , Rosane Angélica Ligabue , Marcelo Lazzaron Lamers , Marcel Ferreira Kunrath

The development of three-dimensional (3D) biomimetic scaffolds for bone and soft tissue engineering has received increasing attention due to their ability to mimic the extracellular matrix (ECM) environment. This study presents the development and characterization of a 3D collagen matrix incorporating titanate nanotubes (TNT) aiming to improve cell migration and biocompatibility, with potential applications in bioink for bone and soft tissue regeneration. TNT were hydrothermally synthesized, and their properties were characterized using materials analysis techniques. After the incorporation of human fibroblasts into the collagen matrices with or without TNT, biological characterization was performed. The results showed that the incorporation of TNT significantly improved the migration efficiency and directionality compared to collagen-only matrices, which were more evident after long-term incubation. This indicates that the addition of TNT to the collagen matrixes improves the mechanical properties, promotes biocompatibility, and induces a superior environment for cell migration. These findings contribute to the development of new biomaterials for tissue engineering and demonstrate the potential of TNT as a key component of bioengineered biomaterials for bone and soft tissue regeneration.

{"title":"Three-dimensional bioactive collagen scaffolds incorporated with titanate nanotubes for tissue regeneration","authors":"Petra Reinehr , Leonardo Francisco Diel , Fernando Mendonça Diz , Gabriela de Souza Balbinot , Wesley Formentin Monteiro , Rosane Angélica Ligabue , Marcelo Lazzaron Lamers , Marcel Ferreira Kunrath","doi":"10.1016/j.colsurfb.2025.114638","DOIUrl":"10.1016/j.colsurfb.2025.114638","url":null,"abstract":"<div><div>The development of three-dimensional (3D) biomimetic scaffolds for bone and soft tissue engineering has received increasing attention due to their ability to mimic the extracellular matrix (ECM) environment. This study presents the development and characterization of a 3D collagen matrix incorporating titanate nanotubes (TNT) aiming to improve cell migration and biocompatibility, with potential applications in bioink for bone and soft tissue regeneration. TNT were hydrothermally synthesized, and their properties were characterized using materials analysis techniques. After the incorporation of human fibroblasts into the collagen matrices with or without TNT, biological characterization was performed. The results showed that the incorporation of TNT significantly improved the migration efficiency and directionality compared to collagen-only matrices, which were more evident after long-term incubation. This indicates that the addition of TNT to the collagen matrixes improves the mechanical properties, promotes biocompatibility, and induces a superior environment for cell migration. These findings contribute to the development of new biomaterials for tissue engineering and demonstrate the potential of TNT as a key component of bioengineered biomaterials for bone and soft tissue regeneration.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"252 ","pages":"Article 114638"},"PeriodicalIF":5.4,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143716087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bright fluorescent biocompatible Magnozyme nanoclusters for brain-cell in-vivo live imaging

IF 5.4 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2025-03-19 DOI: 10.1016/j.colsurfb.2025.114630

Prachi Srivastava , Vivek Kumar Verma , Abhishesh Kumar Mehata , Mamata Singh , Shivesh Sabbarwal , M.S. Muthu , Biplob Koch , Manoj Kumar

Multifluorescent, water-dispersible magnesium nanoclusters (Magnozyme) were obtained using a simple and economical synthesis procedure. The prepared particles were 4 nm in size, and they exhibited significant emission at 450, 545, and 628 nm with multiple excitations of 366,469 and 560 nm wavelengths. The prepared particle exhibited a maximum absolute quantum yield of 21.3, 6.8 % and 5 % in red, green and blue spectrum, respectively, with excellent photostability, good ionic strength tolerability, and broad-range pH stability. The prepared Magnozyme demonstrates 95 % cell viability in human glioma brain cell lines (U-87 MG) and can be used as a probe for cellular imaging. Furthermore, imaging with this brain cell revealed significant cytoplasmic accumulation in the red, green, and blue regions. The confocal Z-stack study revealed the presence of Magnozyme at a depth of the cellular level by capturing a series of images at different planer axes (z-axis). Furthermore, In-vivo toxicity assessments and in-vivo imaging in mice revealed the nontoxicity behavior of Magnozyme with their great staining ability in physiological conditions, confirming their candidature toward biological cell imaging/labeling purposes.

{"title":"Bright fluorescent biocompatible Magnozyme nanoclusters for brain-cell in-vivo live imaging","authors":"Prachi Srivastava , Vivek Kumar Verma , Abhishesh Kumar Mehata , Mamata Singh , Shivesh Sabbarwal , M.S. Muthu , Biplob Koch , Manoj Kumar","doi":"10.1016/j.colsurfb.2025.114630","DOIUrl":"10.1016/j.colsurfb.2025.114630","url":null,"abstract":"<div><div>Multifluorescent, water-dispersible magnesium nanoclusters (Magnozyme) were obtained using a simple and economical synthesis procedure. The prepared particles were 4 nm in size, and they exhibited significant emission at 450, 545, and 628 nm with multiple excitations of 366,469 and 560 nm wavelengths. The prepared particle exhibited a maximum absolute quantum yield of 21.3, 6.8 % and 5 % in red, green and blue spectrum, respectively, with excellent photostability, good ionic strength tolerability, and broad-range pH stability. The prepared Magnozyme demonstrates 95 % cell viability in human glioma brain cell lines (U-87 MG) and can be used as a probe for cellular imaging. Furthermore, imaging with this brain cell revealed significant cytoplasmic accumulation in the red, green, and blue regions. The confocal Z-stack study revealed the presence of Magnozyme at a depth of the cellular level by capturing a series of images at different planer axes (z-axis). Furthermore, <em>In-vivo</em> toxicity assessments and <em>in-vivo</em> imaging in mice revealed the nontoxicity behavior of Magnozyme with their great staining ability in physiological conditions, confirming their candidature toward biological cell imaging/labeling purposes.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"252 ","pages":"Article 114630"},"PeriodicalIF":5.4,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143704481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of copper content in Ti-Cu alloy with bone regeneration ability on the phenotypic transformation of macrophages

IF 5.4 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2025-03-19 DOI: 10.1016/j.colsurfb.2025.114641

Yueyang Qiu , Hui Liu , Chengwei Han , Zhuoqun Yan , Yanjin Lu , Ling Ren , Qiang Wang , Qing Zhou , Lei Xue

Titanium (Ti) alloys are widely used in bone repair due to their excellent biocompatibility and mechanical properties. However, managing post-implantation inflammatory responses in the defect region and accelerating the healing process remain major challenges in the design of such materials. As a bridge between the innate and adaptive immune systems, macrophages play a pivotal role in bone defect healing through their M2 polarization, which facilitates the secretion of tissue repair-promoting cytokines. Research on the role of copper ions (Cu²⁺) in regulating inflammatory responses at injury sites suggests their potential as active ions for incorporation into alloys as a secondary phase to modulate macrophage polarization. However, the effective concentration and mechanisms in this process remain unclear. Here, we synthesized Ti-xCu (x = 3, 5, 7 wt%) alloys and investigated the effects of copper concentration on macrophage M1/M2 polarization and the underlying mechanisms. In an 8-week rat mandibular bone regeneration experiment, Ti-5Cu demonstrated superior performance compared to pure titanium. At the early stage (2 weeks), Ti-5Cu promoted the dominance of M1 macrophages and upregulated inflammatory cytokines, facilitating the initial inflammatory response. Subsequently, a timely M1-to-M2 phenotype transition was observed, accompanied by elevated expression of the repair-related cytokine IL-10, ultimately leading to improved bone healing. This study provides a theoretical foundation for the development of titanium-copper composite materials with anti-inflammatory and pro-healing properties, paving the way for innovative solutions to promote bone defect repair.

{"title":"The effect of copper content in Ti-Cu alloy with bone regeneration ability on the phenotypic transformation of macrophages","authors":"Yueyang Qiu , Hui Liu , Chengwei Han , Zhuoqun Yan , Yanjin Lu , Ling Ren , Qiang Wang , Qing Zhou , Lei Xue","doi":"10.1016/j.colsurfb.2025.114641","DOIUrl":"10.1016/j.colsurfb.2025.114641","url":null,"abstract":"<div><div>Titanium (Ti) alloys are widely used in bone repair due to their excellent biocompatibility and mechanical properties. However, managing post-implantation inflammatory responses in the defect region and accelerating the healing process remain major challenges in the design of such materials. As a bridge between the innate and adaptive immune systems, macrophages play a pivotal role in bone defect healing through their M2 polarization, which facilitates the secretion of tissue repair-promoting cytokines. Research on the role of copper ions (Cu²⁺) in regulating inflammatory responses at injury sites suggests their potential as active ions for incorporation into alloys as a secondary phase to modulate macrophage polarization. However, the effective concentration and mechanisms in this process remain unclear. Here, we synthesized Ti-xCu (x = 3, 5, 7 wt%) alloys and investigated the effects of copper concentration on macrophage M1/M2 polarization and the underlying mechanisms. In an 8-week rat mandibular bone regeneration experiment, Ti-5Cu demonstrated superior performance compared to pure titanium. At the early stage (2 weeks), Ti-5Cu promoted the dominance of M1 macrophages and upregulated inflammatory cytokines, facilitating the initial inflammatory response. Subsequently, a timely M1-to-M2 phenotype transition was observed, accompanied by elevated expression of the repair-related cytokine IL-10, ultimately leading to improved bone healing. This study provides a theoretical foundation for the development of titanium-copper composite materials with anti-inflammatory and pro-healing properties, paving the way for innovative solutions to promote bone defect repair.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"252 ","pages":"Article 114641"},"PeriodicalIF":5.4,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143704483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ROS-responsive oridonin and dihydroartemisinin hetero-polymeric prodrug NPs for potentiating ferroptosis in gastric cancer by disrupting redox balance

IF 5.4 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2025-03-19 DOI: 10.1016/j.colsurfb.2025.114637

Luzhou Xu , Yan Wang , Yanqin Hu , Xinyi Dai , Cheng Sun , Jun Cheng

Gastric cancer presents a significant global health concern, with conventional therapies often limited in effectiveness. The abnormal redox balance in gastric cancer cells may represent a breakthrough in the treatment of gastric cancer. In this study, we report for the first time the development of reactive oxygen species (ROS)-sensitive hetero-polymeric prodrug nanoparticles (NPs) designed for the co-delivery of the Chinese herbal extract oridonin (ORI) and dihydroartemisinin (DHA) in combination therapy for gastric cancer. This strategy aims to disrupt the intracellular redox balance and ultimately induce ferroptosis in gastric cancer cells. The ROS-responsive ORI and DHA polymeric prodrug were synthesised by conjugating ORI or DHA to poly(ethylene glycol)-block-poly(L-lysine) (PEG-b-PLL) via a ROS-sensitive linker thioketal (TK). The resulting polymeric prodrugs self-assemble in water to form NPs OD-M. After internalization by gastric cancer cells, OD-M released ORI and DHA in response to high ROS conditions within cancer cells. The released ORI reacts with GSH to induce GSH depletion while DHA amplifies intracellular ROS levels, ultimately inducing ferroptosis in gastric cancer cells. Experimental results demonstrate that OD-M acts as both a GSH scavenger and ROS generator, effectively disrupting intracellular redox balance, inducing ferroptosis, and exhibiting effective anticancer efficacy in vitro and in vivo, offering a departure from traditional methods.

{"title":"ROS-responsive oridonin and dihydroartemisinin hetero-polymeric prodrug NPs for potentiating ferroptosis in gastric cancer by disrupting redox balance","authors":"Luzhou Xu , Yan Wang , Yanqin Hu , Xinyi Dai , Cheng Sun , Jun Cheng","doi":"10.1016/j.colsurfb.2025.114637","DOIUrl":"10.1016/j.colsurfb.2025.114637","url":null,"abstract":"<div><div>Gastric cancer presents a significant global health concern, with conventional therapies often limited in effectiveness. The abnormal redox balance in gastric cancer cells may represent a breakthrough in the treatment of gastric cancer. In this study, we report for the first time the development of reactive oxygen species (ROS)-sensitive hetero-polymeric prodrug nanoparticles (NPs) designed for the co-delivery of the Chinese herbal extract oridonin (ORI) and dihydroartemisinin (DHA) in combination therapy for gastric cancer. This strategy aims to disrupt the intracellular redox balance and ultimately induce ferroptosis in gastric cancer cells. The ROS-responsive ORI and DHA polymeric prodrug were synthesised by conjugating ORI or DHA to poly(ethylene glycol)-<em>block</em>-poly(L-lysine) (PEG-<em>b</em>-PLL) <em>via</em> a ROS-sensitive linker thioketal (TK). The resulting polymeric prodrugs self-assemble in water to form NPs OD-M. After internalization by gastric cancer cells, OD-M released ORI and DHA in response to high ROS conditions within cancer cells. The released ORI reacts with GSH to induce GSH depletion while DHA amplifies intracellular ROS levels, ultimately inducing ferroptosis in gastric cancer cells. Experimental results demonstrate that OD-M acts as both a GSH scavenger and ROS generator, effectively disrupting intracellular redox balance, inducing ferroptosis, and exhibiting effective anticancer efficacy <em>in vitro</em> and <em>in vivo</em>, offering a departure from traditional methods.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"252 ","pages":"Article 114637"},"PeriodicalIF":5.4,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143680035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nanosilica cross-linked polyurethane hybrid hydrogels to stabilize the silicone rubber based invasive electrode-neural tissue interface

IF 5.4 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2025-03-19 DOI: 10.1016/j.colsurfb.2025.114643

Haihan Zhou , Kuoying Qiao , Li Rao , Hua-Jin Zhai

An unstable electrode-neural tissue interface induced by tissue inflammatory response hinders the application of invasive brain-computer interfaces (BCIs). In this work, nanosilica cross-linked polyurethane (SiO₂/PU) hybrid hydrogels were prepared to serve as the coatings and to modify silicone rubber (SR), which is a commonly used encapsulation material of invasive electrodes for neural recording/stimulation. The hydrophilicity, swelling ratio, and bulk ionic conductivity of SiO₂/PU hybrid hydrogels were tailored by incorporating different amount of SiO₂ serving as the cross-linking agent. Correspondingly, the optimized SiO₂/PU hybrid hydrogel coatings have less impact on the electrochemical properties of invasive electrodes relative to PU hydrogel. Cell affinity assays with rat pheochromocytoma cells reveal that coatings made of SiO₂/PU hybrid hydrogels are more effective in enhancing their adhesion and neurite outgrowth than those made of PU hydrogels. The adsorption amount of non-specific proteins on SR is significantly reduced by 81.6 % and 92.6 % upon coating with PU hydrogels and SiO₂/PU hybrid hydrogels, respectively. Histological assessment indicates that the SR implants with a SiO₂/PU hybrid hydrogel coating provoke the mildest tissue response. Collectively, the SiO₂/PU hybrid hydrogel is highly promising for the stabilization of electrode-neural tissue interface, which is crucial for the development of invasive BCIs.

{"title":"Nanosilica cross-linked polyurethane hybrid hydrogels to stabilize the silicone rubber based invasive electrode-neural tissue interface","authors":"Haihan Zhou , Kuoying Qiao , Li Rao , Hua-Jin Zhai","doi":"10.1016/j.colsurfb.2025.114643","DOIUrl":"10.1016/j.colsurfb.2025.114643","url":null,"abstract":"<div><div>An unstable electrode-neural tissue interface induced by tissue inflammatory response hinders the application of invasive brain-computer interfaces (BCIs). In this work, nanosilica cross-linked polyurethane (SiO<sub>2</sub>/PU) hybrid hydrogels were prepared to serve as the coatings and to modify silicone rubber (SR), which is a commonly used encapsulation material of invasive electrodes for neural recording/stimulation. The hydrophilicity, swelling ratio, and bulk ionic conductivity of SiO<sub>2</sub>/PU hybrid hydrogels were tailored by incorporating different amount of SiO<sub>2</sub> serving as the cross-linking agent. Correspondingly, the optimized SiO<sub>2</sub>/PU hybrid hydrogel coatings have less impact on the electrochemical properties of invasive electrodes relative to PU hydrogel. Cell affinity assays with rat pheochromocytoma cells reveal that coatings made of SiO<sub>2</sub>/PU hybrid hydrogels are more effective in enhancing their adhesion and neurite outgrowth than those made of PU hydrogels. The adsorption amount of non-specific proteins on SR is significantly reduced by 81.6 % and 92.6 % upon coating with PU hydrogels and SiO<sub>2</sub>/PU hybrid hydrogels, respectively. Histological assessment indicates that the SR implants with a SiO<sub>2</sub>/PU hybrid hydrogel coating provoke the mildest tissue response. Collectively, the SiO<sub>2</sub>/PU hybrid hydrogel is highly promising for the stabilization of electrode-neural tissue interface, which is crucial for the development of invasive BCIs.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"251 ","pages":"Article 114643"},"PeriodicalIF":5.4,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143687787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

On-demand dual-stimuli-responsive hydrogels for localized and sustained delivery of MP-L [I5R8] to treat bacterial wound infections

IF 5.4 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2025-03-18 DOI: 10.1016/j.colsurfb.2025.114636

Marcelo Guerrero , Adolfo Marican , Diana Rafael , Fernanda Andrade , Rodrigo Moore-Carrasco , Sekar Vijayakumar , Paulo Salinas , Gustavo Cabrera-Barjas , Juan Lara , Esteban F. Durán-Lara

This study presents the development of two novel injectable dual-responsive polyanionic hydrogels (DRPHs) based on N-isopropylacrylamide (NIPAM), incorporating carboxylic acid comonomers for temperature- and pH-responsive drug release. These hydrogels were designed for the sustained and localized delivery of the antimicrobial peptide MP-L [I5R8], targeting multidrug-resistant bacteria (MDRB) in wound infections. The physicochemical characterization confirmed polymer formation and comonomer integration through Fourier-transform infrared spectroscopy (FTIR) and nuclear magnetic resonance (NMR). Rheological analysis demonstrated a temperature-dependent sol-gel transition at ∼35°C, making the hydrogels suitable for in situ gelation at physiological conditions. The hydrogels exhibited tunable swelling behavior and a controlled dual-phase release profile of MP-L [I5R8], ensuring both immediate bactericidal activity and prolonged antimicrobial effect. In vitro assays confirmed sustained antimicrobial efficacy against Staphylococcus aureus and Pseudomonas aeruginosa, while biocompatibility tests validated their safety for biomedical applications. An in vivo diabetic wound infection model demonstrated rapid infection clearance, enhanced wound healing, and organized tissue regeneration following treatment with MP-L [I5R8]-loaded DRPHs. These results highlight the potential of dual-stimuli-responsive hydrogels as a next-generation antimicrobial delivery platform for the treatment of chronic infected wounds, such as diabetic foot ulcers.

{"title":"On-demand dual-stimuli-responsive hydrogels for localized and sustained delivery of MP-L [I5R8] to treat bacterial wound infections","authors":"Marcelo Guerrero , Adolfo Marican , Diana Rafael , Fernanda Andrade , Rodrigo Moore-Carrasco , Sekar Vijayakumar , Paulo Salinas , Gustavo Cabrera-Barjas , Juan Lara , Esteban F. Durán-Lara","doi":"10.1016/j.colsurfb.2025.114636","DOIUrl":"10.1016/j.colsurfb.2025.114636","url":null,"abstract":"<div><div>This study presents the development of two novel injectable dual-responsive polyanionic hydrogels (DRPHs) based on N-isopropylacrylamide (NIPAM), incorporating carboxylic acid comonomers for temperature- and pH-responsive drug release. These hydrogels were designed for the sustained and localized delivery of the antimicrobial peptide MP-L [I5R8], targeting multidrug-resistant bacteria (MDRB) in wound infections. The physicochemical characterization confirmed polymer formation and comonomer integration through Fourier-transform infrared spectroscopy (FTIR) and nuclear magnetic resonance (NMR). Rheological analysis demonstrated a temperature-dependent sol-gel transition at ∼35°C, making the hydrogels suitable for <em>in situ</em> gelation at physiological conditions. The hydrogels exhibited tunable swelling behavior and a controlled dual-phase release profile of MP-L [I5R8], ensuring both immediate bactericidal activity and prolonged antimicrobial effect. <em>In vitro</em> assays confirmed sustained antimicrobial efficacy against <em>Staphylococcus aureus</em> and <em>Pseudomonas aeruginosa</em>, while biocompatibility tests validated their safety for biomedical applications. An <em>in vivo</em> diabetic wound infection model demonstrated rapid infection clearance, enhanced wound healing, and organized tissue regeneration following treatment with MP-L [I5R8]-loaded DRPHs. These results highlight the potential of dual-stimuli-responsive hydrogels as a next-generation antimicrobial delivery platform for the treatment of chronic infected wounds, such as diabetic foot ulcers.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"251 ","pages":"Article 114636"},"PeriodicalIF":5.4,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143673038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microenvironment responsive nanomedicine for acute pancreatitis treatment

IF 5.4 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2025-03-13 DOI: 10.1016/j.colsurfb.2025.114633

Yixuan Zhu , Qian Wang , Yaoyao Zheng , Nuo Chen , Longfa Kou , Qing Yao

Acute pancreatitis (AP) is an acute inflammation of the pancreas, which is considered a prevalent gastrointestinal emergency characterized by rapid progression and significant mortality. Currently available medications primarily serve as adjunctive therapies, yielding suboptimal therapeutic outcomes. Consequently, there remains a dearth of specific and efficient treatment modalities for AP. In recent years, nanomedicine-based treatment strategies have exhibited significant potential as drug therapy approaches for pancreatitis. The distinctive features of the AP microenvironment encompass aberrant activation of pancreatic enzymes, oxidative stress induced by elevated reactive oxygen species levels, and excessive production of pro-inflammatory cytokines; these factors offer promising targeted sites for early diagnosis and treatment using nanomedicine. This article comprehensively delineates the pathological microenvironmental characteristics associated with AP while highlighting the application of microenvironment-responsive strategies in nanodrug delivery systems for its treatment, thereby providing insights into future prospects.

{"title":"Microenvironment responsive nanomedicine for acute pancreatitis treatment","authors":"Yixuan Zhu , Qian Wang , Yaoyao Zheng , Nuo Chen , Longfa Kou , Qing Yao","doi":"10.1016/j.colsurfb.2025.114633","DOIUrl":"10.1016/j.colsurfb.2025.114633","url":null,"abstract":"<div><div>Acute pancreatitis (AP) is an acute inflammation of the pancreas, which is considered a prevalent gastrointestinal emergency characterized by rapid progression and significant mortality. Currently available medications primarily serve as adjunctive therapies, yielding suboptimal therapeutic outcomes. Consequently, there remains a dearth of specific and efficient treatment modalities for AP. In recent years, nanomedicine-based treatment strategies have exhibited significant potential as drug therapy approaches for pancreatitis. The distinctive features of the AP microenvironment encompass aberrant activation of pancreatic enzymes, oxidative stress induced by elevated reactive oxygen species levels, and excessive production of pro-inflammatory cytokines; these factors offer promising targeted sites for early diagnosis and treatment using nanomedicine. This article comprehensively delineates the pathological microenvironmental characteristics associated with AP while highlighting the application of microenvironment-responsive strategies in nanodrug delivery systems for its treatment, thereby providing insights into future prospects.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"251 ","pages":"Article 114633"},"PeriodicalIF":5.4,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143644647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Surface-modified nintedanib-loaded solid lipid nanoparticles for effective targeting of non-small cell lung cancer

IF 5.4 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2025-03-13 DOI: 10.1016/j.colsurfb.2025.114622

Shubhangi Nalawade , Mahavir Narwade , Vishambhar Deshmukh , Nazim Nasir , Shadma Wahab , Prashant Kesharwani , Kavita R. Gajbhiye

Lung cancer remains a significant global health burden as the second most common and fatal malignancy, with treatment complexities heightened by limited knowledge of inhaler techniques and respiratory challenges, particularly in elderly and pediatric patients. Despite the availability of oral chemotherapeutics like Nintedanib, its clinical efficacy is undermined by suboptimal pharmacokinetics, high systemic toxicity, and low bioavailability. To overcome these limitations, we developed folic acid-conjugated Nintedanib-loaded solid lipid nanoparticles (FA-NIN-SLNPs), which offer targeted therapy with enhanced delivery and reduced adverse effects, potentially improving patient adherence. Prepared through a refined nanoprecipitation and self-assembly method, FA-NIN-SLNPs exhibited a particle size of 220.5 ± 6.08 nm, a zeta potential of 32.1 ± 3.05 mV, and an entrapment efficiency of 98.3 ± 0.80 %. In vitro release studies indicated accelerated drug release at acidic tumor pH, with FA-NIN-SLNPs showing significantly enhanced apoptosis (86.65 %) in A549 lung cancer cells versus NIN-SLNPs (67.65 %) and free drug (23.53 %). Cellular uptake assays highlighted its targeted capabilities, while histopathological and hemolysis assessments confirmed its safety profile. In vivo pharmacokinetic and biodistribution studies further demonstrated superior lung-specific accumulation, positioning this nanoformulation as a promising, safer, and more efficacious approach for targeted lung cancer therapy.

{"title":"Surface-modified nintedanib-loaded solid lipid nanoparticles for effective targeting of non-small cell lung cancer","authors":"Shubhangi Nalawade , Mahavir Narwade , Vishambhar Deshmukh , Nazim Nasir , Shadma Wahab , Prashant Kesharwani , Kavita R. Gajbhiye","doi":"10.1016/j.colsurfb.2025.114622","DOIUrl":"10.1016/j.colsurfb.2025.114622","url":null,"abstract":"<div><div>Lung cancer remains a significant global health burden as the second most common and fatal malignancy, with treatment complexities heightened by limited knowledge of inhaler techniques and respiratory challenges, particularly in elderly and pediatric patients. Despite the availability of oral chemotherapeutics like Nintedanib, its clinical efficacy is undermined by suboptimal pharmacokinetics, high systemic toxicity, and low bioavailability. To overcome these limitations, we developed folic acid-conjugated Nintedanib-loaded solid lipid nanoparticles (FA-NIN-SLNPs), which offer targeted therapy with enhanced delivery and reduced adverse effects, potentially improving patient adherence. Prepared through a refined nanoprecipitation and self-assembly method, FA-NIN-SLNPs exhibited a particle size of 220.5 ± 6.08 nm, a zeta potential of 32.1 ± 3.05 mV, and an entrapment efficiency of 98.3 ± 0.80 %. <em>In vitro</em> release studies indicated accelerated drug release at acidic tumor pH, with FA-NIN-SLNPs showing significantly enhanced apoptosis (86.65 %) in A549 lung cancer cells versus NIN-SLNPs (67.65 %) and free drug (23.53 %). Cellular uptake assays highlighted its targeted capabilities, while histopathological and hemolysis assessments confirmed its safety profile. <em>In vivo</em> pharmacokinetic and biodistribution studies further demonstrated superior lung-specific accumulation, positioning this nanoformulation as a promising, safer, and more efficacious approach for targeted lung cancer therapy.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"251 ","pages":"Article 114622"},"PeriodicalIF":5.4,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143644648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Construction of hyaluronic acid/ZnO nanocubes and their pH-responsive stability in drug delivery

IF 5.4 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2025-03-13 DOI: 10.1016/j.colsurfb.2025.114632

Xueqing Li , Yulong Yan , Xubo Zhao

Hyaluronic acid (HA), a naturally occurring polysaccharide, is extensively utilized in the biomedical field owing to its excellent biocompatibility. However, assembling HA directly into nanomaterials with tunable stability remains challenging, primarily due to its hydrophilic nature. In this study, we introduce a novel method for inducing HA assembly through in-situ formation of ZnO nanoparticles to develop HA-based nanomaterials, specifically HA/ZnO nanocubes (HA/ZnO NCs). Following doxorubicin (DOX) loading, the DOX-loaded HA/ZnO NCs exhibit remarkable structural stability under normal physiological conditions and demonstrate acid-responsive dissociation within the tumor microenvironment. In vitro results confirm that HA/ZnO NCs possess excellent biocompatibility, while the DOX-loaded HA/ZnO NCs effectively inhibit tumor cell viability. Consequently, the integration of HA and ZnO represents a promising strategy for enhancing HA-based drug delivery systems (DDSs).

引用次数: 0

Exploring the synergy between bioluminescence and nanomaterials: Innovations in analytical and therapeutic applications

IF 5.4 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2025-03-13 DOI: 10.1016/j.colsurfb.2025.114631

Polina M. Ivantcova , Adilya R. Sungatullina , Kristina V. Pidgirnaya , Maxim P. Nikitin

The application of bioluminescent luciferin-luciferase systems for visualizing and stimulating various processes in living systems is of great interest due to its specific nature and high signal-to-noise ratio. Nanomaterials can finely manipulate multiple parameters of the bioluminescent systems, including the enzyme stability, intensity, and duration of the irradiation. Also, bioluminescence can affect the properties of a nanomaterial, namely, to carry out BRET, to trigger cascades of various photochemical transformations. Here we summarize cases of the interplay between nanomaterials and various bioluminescent systems to improve various biosensors, biovisualization in cellulo, in vivo, and for therapy over the past twenty years. We reviewed interactions between a wide range of nanomaterials and bioluminescent systems, including bacterial and genetically encoded luciferases. This review aims to serve as a comprehensive guide for developing bioluminescent multimodal nanoplatforms for analytic applications and therapy.

{"title":"Exploring the synergy between bioluminescence and nanomaterials: Innovations in analytical and therapeutic applications","authors":"Polina M. Ivantcova , Adilya R. Sungatullina , Kristina V. Pidgirnaya , Maxim P. Nikitin","doi":"10.1016/j.colsurfb.2025.114631","DOIUrl":"10.1016/j.colsurfb.2025.114631","url":null,"abstract":"<div><div>The application of bioluminescent luciferin-luciferase systems for visualizing and stimulating various processes in living systems is of great interest due to its specific nature and high signal-to-noise ratio. Nanomaterials can finely manipulate multiple parameters of the bioluminescent systems, including the enzyme stability, intensity, and duration of the irradiation. Also, bioluminescence can affect the properties of a nanomaterial, namely, to carry out BRET, to trigger cascades of various photochemical transformations. Here we summarize cases of the interplay between nanomaterials and various bioluminescent systems to improve various biosensors, biovisualization <em>in cellulo</em>, <em>in vivo</em>, and for therapy over the past twenty years. We reviewed interactions between a wide range of nanomaterials and bioluminescent systems, including bacterial and genetically encoded luciferases. This review aims to serve as a comprehensive guide for developing bioluminescent multimodal nanoplatforms for analytic applications and therapy.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"251 ","pages":"Article 114631"},"PeriodicalIF":5.4,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143687758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

首页上一页

下一页尾页

类型

全部化学•材料生命科学医学物理工程技术环境•农林材料科学地球科学法学管理学化学环境科学与生态学计算机科学教育学经济学农林科学人文科学生物学数学物理与天体物理心理学综合性期刊其他工业工程理学历史学农学文学信息工程

数据库

全部 ACS Publications Elsevier ieeexplore Springer The Royal Society of Chemistry Wiley

期刊

Colloids and Surfaces B: Biointerfaces

全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.

﹀