Pub Date : 2026-04-01Epub Date: 2025-12-23DOI: 10.1016/j.colsurfb.2025.115349
Haiyue Ren , Zhicheng Pan , Jinfeng Yuan , Jing Liu
Glutaraldehyde cross-linked bovine/porcine pericardium has been used as prosthetic heart valves (BHVs), while residual aldehyde groups induce cytotoxicity, thrombus formation, and progressive calcification deposition, which ultimately limits instrument life span to 10–15 years. We innovatively designed a multifunctional zwitterionic hydrogel coating copolymer poly (SBMA-co-DMEMAm) on swim bladder-derived material, which is composed of hydrophilic monomer SBMA and aldehyde-containing monomer DMEMAm; besides the aldehyde group can crosslink fish bladder tissue and improve the mechanical properties. This multifunctional hydrophilic polymer coatings provide effective anti-fouling properties, better anti-platelet adsorption and anti-thrombotic properties in in vitro blood compatibility evaluation. In addition, it showed better anti-inflammatory and anti-calcification properties in rat subcutaneous implantation. Thus, this study presents a versatile zwitterionic hydrogel coating strategy that simultaneously endows decellularized swim bladder with superior anti-fouling, hemocompatibility, cytocompatibility, and anti-thrombogenicity, offering a comprehensive solution for durable bioprosthetic heart valves.
{"title":"Zwitterionic hydrogel coatings enhance anti-fouling and anti-thrombogenicity of decellularized swim bladder for bioprosthetic heart valves","authors":"Haiyue Ren , Zhicheng Pan , Jinfeng Yuan , Jing Liu","doi":"10.1016/j.colsurfb.2025.115349","DOIUrl":"10.1016/j.colsurfb.2025.115349","url":null,"abstract":"<div><div>Glutaraldehyde cross-linked bovine/porcine pericardium has been used as prosthetic heart valves (BHVs), while residual aldehyde groups induce cytotoxicity, thrombus formation, and progressive calcification deposition, which ultimately limits instrument life span to 10–15 years. We innovatively designed a multifunctional zwitterionic hydrogel coating copolymer poly (SBMA-co-DMEMAm) on swim bladder-derived material, which is composed of hydrophilic monomer SBMA and aldehyde-containing monomer DMEMAm; besides the aldehyde group can crosslink fish bladder tissue and improve the mechanical properties. This multifunctional hydrophilic polymer coatings provide effective anti-fouling properties, better anti-platelet adsorption and anti-thrombotic properties in <em>in vitro</em> blood compatibility evaluation. In addition, it showed better anti-inflammatory and anti-calcification properties in rat subcutaneous implantation. Thus, this study presents a versatile zwitterionic hydrogel coating strategy that simultaneously endows decellularized swim bladder with superior anti-fouling, hemocompatibility, cytocompatibility, and anti-thrombogenicity, offering a comprehensive solution for durable bioprosthetic heart valves.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"260 ","pages":"Article 115349"},"PeriodicalIF":5.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145831802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-04-01Epub Date: 2025-12-15DOI: 10.1016/j.colsurfb.2025.115375
Siyu Du , Yi Zheng , Yabing Chen , Yijing Guo , Xinyue Kang , Lei Wang
The growing incidence of periodontitis demands innovative therapies, as current drug treatments are limited by short retention times, rapid clearance, and the risk of antimicrobial resistance. This study introduces a novel pH-responsive nano-miclle system for simvastatin (SIM) delivery. Its core innovation lies in being the first system ((SIM NP)n) of its kind to be stabilized by a cross-linked "outer shell," which significantly enhances its stability and controlled release capabilities. This "smart" system remains stable in a neutral environment (pH 7.4), preventing premature drug release. However, upon encountering the acidic inflammatory microenvironment of periodontitis, the shell degrades, triggering the sustained release of SIM directly at the site of inflammation. In vitro studies demonstrated that the SIM-loaded micelles possess potent immunomodulatory effects: they effectively suppressed pro-inflammatory M1 macrophage polarization (decreasing IL-1β, iNOS) while promoting anti-inflammatory M2 macrophage polarization (increasing Arg-1, IL-10). Mechanistic investigation confirmed this therapeutic effect is mediated by the PI3K/AKT/mTOR signaling pathway. More importantly, in a mouse model of periodontitis, the nano-micelles significantly reduced alveolar bone resorption, demonstrating potent anti-inflammatory and bone-protective efficacy in vivo. In conclusion, this "smart" pH-triggered delivery system for SIM offers a highly promising and effective strategy to overcome the limitations of traditional therapies, providing a new targeted path for the treatment of periodontitis.
{"title":"Fighting periodontitis with a pH-triggered nanocoating: A sustained-release strategy for simvastatin delivery","authors":"Siyu Du , Yi Zheng , Yabing Chen , Yijing Guo , Xinyue Kang , Lei Wang","doi":"10.1016/j.colsurfb.2025.115375","DOIUrl":"10.1016/j.colsurfb.2025.115375","url":null,"abstract":"<div><div>The growing incidence of periodontitis demands innovative therapies, as current drug treatments are limited by short retention times, rapid clearance, and the risk of antimicrobial resistance. This study introduces a novel pH-responsive nano-miclle system for simvastatin (SIM) delivery. Its core innovation lies in being the first system ((SIM NP)n) of its kind to be stabilized by a cross-linked \"outer shell,\" which significantly enhances its stability and controlled release capabilities. This \"smart\" system remains stable in a neutral environment (pH 7.4), preventing premature drug release. However, upon encountering the acidic inflammatory microenvironment of periodontitis, the shell degrades, triggering the sustained release of SIM directly at the site of inflammation. In vitro studies demonstrated that the SIM-loaded micelles possess potent immunomodulatory effects: they effectively suppressed pro-inflammatory M1 macrophage polarization (decreasing IL-1β, iNOS) while promoting anti-inflammatory M2 macrophage polarization (increasing Arg-1, IL-10). Mechanistic investigation confirmed this therapeutic effect is mediated by the PI3K/AKT/mTOR signaling pathway. More importantly, in a mouse model of periodontitis, the nano-micelles significantly reduced alveolar bone resorption, demonstrating potent anti-inflammatory and bone-protective efficacy in vivo. In conclusion, this \"smart\" pH-triggered delivery system for SIM offers a highly promising and effective strategy to overcome the limitations of traditional therapies, providing a new targeted path for the treatment of periodontitis.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"260 ","pages":"Article 115375"},"PeriodicalIF":5.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145792768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-04-01Epub Date: 2026-01-06DOI: 10.1016/j.colsurfb.2026.115419
Xiaoyang Xu , Danni Wang , Bingjie Leng , Tingting Gao , Zhenning Cui , Xiao Wang , Bin Liu
Sonodynamic antimicrobial chemotherapy (SACT) has been regarded as an emerging approach for antimicrobial treatment due to its advantages of deep tissue penetration, non-invasive nature, and absence of resistance. However, several challenges hinder its clinical application, for example insufficient oxygen levels at infection sites, poor solubility and stability of sonosensitizers. Herein, we developed chlorin e6@ZIF-8@Ag (CZ@Ag) nanocomposite as a Schottky heterojunction sonosensitizer with high electron-hole separation and narrow bandgap. The results reveal that the Schottky barrier between ZIF-8 and Ag nanoparticles can availably restrain electron backflow, further promoting the yield of reactive oxygen species (ROS) under ultrasound treatment. Experimentally, the minimum inhibitory concentration of CZ@Ag against methicillin-resistant Staphylococcus aureus (MRSA) reduced from 320 μg/mL to 80 μg/mL, and its antibacterial rate was up to 96.3 % after 3 min of ultrasound irradiation. Furthermore, mechanistic studies found that CZ@Ag-mediated SACT is mainly achieved by inhibiting biofilm formation, damaging cell membrane integrity, enhancing more ROS generation, and increasing cell membrane permeability, leading to the leakage of intracellular contents. All these findings proved that CZ@Ag had great possibility for antibacterial therapy, and this investigation offered a feasible direction for developing efficient sonosensitizers.
{"title":"ZIF-8-based Schottky heterojunction for boosting the sonodynamic antimicrobial effect of chlorin e6 on MRSA","authors":"Xiaoyang Xu , Danni Wang , Bingjie Leng , Tingting Gao , Zhenning Cui , Xiao Wang , Bin Liu","doi":"10.1016/j.colsurfb.2026.115419","DOIUrl":"10.1016/j.colsurfb.2026.115419","url":null,"abstract":"<div><div>Sonodynamic antimicrobial chemotherapy (SACT) has been regarded as an emerging approach for antimicrobial treatment due to its advantages of deep tissue penetration, non-invasive nature, and absence of resistance. However, several challenges hinder its clinical application, for example insufficient oxygen levels at infection sites, poor solubility and stability of sonosensitizers. Herein, we developed chlorin e6@ZIF-8@Ag (CZ@Ag) nanocomposite as a Schottky heterojunction sonosensitizer with high electron-hole separation and narrow bandgap. The results reveal that the Schottky barrier between ZIF-8 and Ag nanoparticles can availably restrain electron backflow, further promoting the yield of reactive oxygen species (ROS) under ultrasound treatment. Experimentally, the minimum inhibitory concentration of CZ@Ag against methicillin-resistant <em>Staphylococcus aureus</em> (MRSA) reduced from 320 μg/mL to 80 μg/mL, and its antibacterial rate was up to 96.3 % after 3 min of ultrasound irradiation. Furthermore, mechanistic studies found that CZ@Ag-mediated SACT is mainly achieved by inhibiting biofilm formation, damaging cell membrane integrity, enhancing more ROS generation, and increasing cell membrane permeability, leading to the leakage of intracellular contents. All these findings proved that CZ@Ag had great possibility for antibacterial therapy, and this investigation offered a feasible direction for developing efficient sonosensitizers.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"260 ","pages":"Article 115419"},"PeriodicalIF":5.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145939093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-04-01Epub Date: 2025-12-24DOI: 10.1016/j.colsurfb.2025.115398
Cristina Chirizzi , Martina Maffezzini , Nadia Mosca , Arianna Balestri , Costanza Montis , Francesco Acerbi , Martina Beccalli , Alessandro Gori , Marina Grisoli , Serena Pellegatta , Francesca Baldelli Bombelli
Glioblastoma (GB) is the most aggressive and common malignant brain tumor, and despite current therapeutic approaches, prognosis remains poor. Given that surgical resection is frequently the sole potentially curative option, precise intraoperative tumor delineation is crucial for reducing recurrence rates and enhancing patient outcomes. In this study, we developed a novel pH-responsive imaging tool by coupling the pH-low insertion peptide (pHLIP) with fluorescein (FL) to enable targeted fluorescence-guided visualization of tumor margins. We investigated pHLIP–lipid membrane interactions using model systems, including liposomes and supported lipid bilayers (SLB), assessing peptide’s pH-dependent insertion mechanism. Complementary in vitro experiments on patient-derived GB cell lines were performed to show the tracer’s pH sensitivity, selective membrane targeting, and potential off-target effects. The FL-pHLIP construct showed robust, pH-dependent membrane insertion and selectivity in GB cellular models with minimal interaction under physiological conditions. These findings support FL-pHLIP as a promising candidate for fluorescence-guided surgery in GB and highlight its potential for clinical translation and for the broader development of pH-responsive diagnostic tools.
{"title":"A fluorescein-tagged pH-low insertion peptide (pHLIP) for glioblastoma imaging: Lipid membrane interaction and cancer cell targeting","authors":"Cristina Chirizzi , Martina Maffezzini , Nadia Mosca , Arianna Balestri , Costanza Montis , Francesco Acerbi , Martina Beccalli , Alessandro Gori , Marina Grisoli , Serena Pellegatta , Francesca Baldelli Bombelli","doi":"10.1016/j.colsurfb.2025.115398","DOIUrl":"10.1016/j.colsurfb.2025.115398","url":null,"abstract":"<div><div>Glioblastoma (GB) is the most aggressive and common malignant brain tumor, and despite current therapeutic approaches, prognosis remains poor. Given that surgical resection is frequently the sole potentially curative option, precise intraoperative tumor delineation is crucial for reducing recurrence rates and enhancing patient outcomes. In this study, we developed a novel pH-responsive imaging tool by coupling the pH-low insertion peptide (pHLIP) with fluorescein (FL) to enable targeted fluorescence-guided visualization of tumor margins. We investigated pHLIP–lipid membrane interactions using model systems, including liposomes and supported lipid bilayers (SLB), assessing peptide’s pH-dependent insertion mechanism. Complementary <em>in vitro</em> experiments on patient-derived GB cell lines were performed to show the tracer’s pH sensitivity, selective membrane targeting, and potential off-target effects. The FL-pHLIP construct showed robust, pH-dependent membrane insertion and selectivity in GB cellular models with minimal interaction under physiological conditions. These findings support FL-pHLIP as a promising candidate for fluorescence-guided surgery in GB and highlight its potential for clinical translation and for the broader development of pH-responsive diagnostic tools.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"260 ","pages":"Article 115398"},"PeriodicalIF":5.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145877215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-04-01Epub Date: 2025-12-28DOI: 10.1016/j.colsurfb.2025.115405
Shining Li , Ying Luo , Dan Wang , Bingqing Wang , Jun Gao , Xin Chen , Zhigang Li , Haixiao Wan , Ying Wu , Zhuang Ma , Yongjie Lu , Lin Geng , Yan Huang , Na Li , Li Ye , Dongcui Li , Naisheng Jiang
Skin aging is closely linked to mitochondrial dysfunction, yet effective delivery of mitochondrial therapeutics to the skin remains a challenge. Here, we report ECG-Lipo, a mitochondria-modulating nanoliposome system that co-delivers hydrophilic ergothioneine (EGT) and lipophilic coenzyme Q10 (CoQ10) for rejuvenation therapy. ECG-Lipo was fabricated by flash nanoprecipitation using 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and protopanaxatriol-type ginsenoside metabolites (PPTGM) as liposome-forming agents, yielding uniform nanosized unilamellar vesicles with high stability and encapsulation efficiency. Compared with free drug solutions, ECG-Lipo significantly enhanced skin penetration and cellular repair, as demonstrated by in vitro Franz diffusion assays, in vivo fluorescence imaging in mouse skin, and fibroblast migration assays. Molecular docking indicated that PPTGM exhibits stronger predicted interactions with the EGT transporter OCTN-1 than cholesterol through combined hydrophobic and hydrogen-bonding interactions, suggesting a potential structural basis for the observed biological effects. In oxidative stress-challenged human dermal fibroblasts, ECG-Lipo preserved mitochondrial integrity by maintaining mitochondrial signal and morphology, restoring membrane potential, and suppressing mitochondrial superoxide accumulation. These results highlight ECG-Lipo as a promising mitochondria-modulating nanoliposome system for transdermal delivery with potential for therapeutic intervention against intrinsic skin aging.
{"title":"Ginsenoside-based nanoliposomes co-delivering ergothioneine and coenzyme Q10 to combat skin aging via mitochondrial modulation","authors":"Shining Li , Ying Luo , Dan Wang , Bingqing Wang , Jun Gao , Xin Chen , Zhigang Li , Haixiao Wan , Ying Wu , Zhuang Ma , Yongjie Lu , Lin Geng , Yan Huang , Na Li , Li Ye , Dongcui Li , Naisheng Jiang","doi":"10.1016/j.colsurfb.2025.115405","DOIUrl":"10.1016/j.colsurfb.2025.115405","url":null,"abstract":"<div><div>Skin aging is closely linked to mitochondrial dysfunction, yet effective delivery of mitochondrial therapeutics to the skin remains a challenge. Here, we report ECG-Lipo, a mitochondria-modulating nanoliposome system that co-delivers hydrophilic ergothioneine (EGT) and lipophilic coenzyme Q10 (CoQ10) for rejuvenation therapy. ECG-Lipo was fabricated by flash nanoprecipitation using 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and protopanaxatriol-type ginsenoside metabolites (PPTGM) as liposome-forming agents, yielding uniform nanosized unilamellar vesicles with high stability and encapsulation efficiency. Compared with free drug solutions, ECG-Lipo significantly enhanced skin penetration and cellular repair, as demonstrated by in vitro Franz diffusion assays, in vivo fluorescence imaging in mouse skin, and fibroblast migration assays. Molecular docking indicated that PPTGM exhibits stronger predicted interactions with the EGT transporter OCTN-1 than cholesterol through combined hydrophobic and hydrogen-bonding interactions, suggesting a potential structural basis for the observed biological effects. In oxidative stress-challenged human dermal fibroblasts, ECG-Lipo preserved mitochondrial integrity by maintaining mitochondrial signal and morphology, restoring membrane potential, and suppressing mitochondrial superoxide accumulation. These results highlight ECG-Lipo as a promising mitochondria-modulating nanoliposome system for transdermal delivery with potential for therapeutic intervention against intrinsic skin aging.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"260 ","pages":"Article 115405"},"PeriodicalIF":5.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145877407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-04-01Epub Date: 2025-12-17DOI: 10.1016/j.colsurfb.2025.115380
Su-Min Lee , Yunyoung Cho , Jiwoo Lim , Seungwoo Chung , Ngoc-Tu Nguyen , Yang-Hoon Kim , Sang-Ho Park , Jiho Min
Yeast vacuoles have recently emerged as promising bio-nanomaterials for drug delivery, offering improved stability and efficacy compared with traditional synthetic systems. Their membranes share structural and compositional similarities with those of mammalian cells, offering excellent biocompatibility and potential for efficient cellular interaction. These unique properties make reassembled vacuoles (ReV) attractive candidates for developing safe and effective therapeutic delivery platforms. This study optimized the vacuole reassembly process to enhance the performance of drug delivery. We compared two methods: Method 1, using conventional long-duration sonication and filtration, and Method 2, featuring an optimized 5-minute sonication without filtration. Reassembled vacuoles produced by method 1 (ReVMtd1), induced moderate TLR2 expression, suggesting mild immune priming without significant activation of inflammatory cytokines. In contrast, Reassembled vacuoles produced by method 2 (ReVMtd2) demonstrated superior immune activation, showing a dose-dependent upregulation of iNOS and TLR2. Additionally, ReVMtd2 achieved an encapsulation efficiency of 12.9 % for daunorubicin (DNR), comparable to native vacuoles, and maintained structural stability over a 12-month period. These findings highlight the potential of ReVMtd2 as a robust, biocompatible, and efficient drug delivery system, offering enhanced therapeutic performance and long-term stability.
{"title":"Fabrication of immune-enhancing vesicles from reassembled yeast vacuolar membranes","authors":"Su-Min Lee , Yunyoung Cho , Jiwoo Lim , Seungwoo Chung , Ngoc-Tu Nguyen , Yang-Hoon Kim , Sang-Ho Park , Jiho Min","doi":"10.1016/j.colsurfb.2025.115380","DOIUrl":"10.1016/j.colsurfb.2025.115380","url":null,"abstract":"<div><div>Yeast vacuoles have recently emerged as promising bio-nanomaterials for drug delivery, offering improved stability and efficacy compared with traditional synthetic systems. Their membranes share structural and compositional similarities with those of mammalian cells, offering excellent biocompatibility and potential for efficient cellular interaction. These unique properties make reassembled vacuoles (ReV) attractive candidates for developing safe and effective therapeutic delivery platforms. This study optimized the vacuole reassembly process to enhance the performance of drug delivery. We compared two methods: Method 1, using conventional long-duration sonication and filtration, and Method 2, featuring an optimized 5-minute sonication without filtration. Reassembled vacuoles produced by method 1 (ReV<sub>Mtd1</sub>), induced moderate TLR2 expression, suggesting mild immune priming without significant activation of inflammatory cytokines. In contrast, Reassembled vacuoles produced by method 2 (ReV<sub>Mtd2</sub>) demonstrated superior immune activation, showing a dose-dependent upregulation of iNOS and TLR2. Additionally, ReV<sub>Mtd2</sub> achieved an encapsulation efficiency of 12.9 % for daunorubicin (DNR), comparable to native vacuoles, and maintained structural stability over a 12-month period. These findings highlight the potential of ReV<sub>Mtd2</sub> as a robust, biocompatible, and efficient drug delivery system, offering enhanced therapeutic performance and long-term stability.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"260 ","pages":"Article 115380"},"PeriodicalIF":5.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145799423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-04-01Epub Date: 2025-12-24DOI: 10.1016/j.colsurfb.2025.115397
Xuechun Kan , Jifei Wang , Yan Li , Dongdong Li , Fan Li , Cheng Huang , Peidang Liu
Radiotherapy is an important part of standard treatment for triple-negative breast cancer (TNBC); however, radioresistance significantly limits therapeutic outcomes. In this study, we developed a novel radiosensitizer based on aptamer-modified silver nanoclusters, NC-T5–5TR1, to potentiate radiotherapy efficacy against TNBC. NC-T5–5TR1 enabled real-time tumor imaging and accurate localization via their intrinsic fluorescence and targeting capability. When combined with ionizing radiation (IR), NC-T5–5TR1 significantly enhanced radiosensitization in vitro, as evidenced by decreased proliferation, increased apoptosis and higher reactive oxygen species (ROS) levels, and also markedly inhibited tumor growth in vivo. Transcriptomic analysis indicated that the IL-6/JAK2/STAT3 signaling pathway was downregulated under NC-T5–5TR1 + IR treatment, which was subsequently validated in vitro and in vivo. Rescue experiments confirmed that IL-6 overexpression reversed NC-T5–5TR1 combined with IR-induced JAK2/STAT3 inhibition and its downstream effects on proliferation, apoptosis, and ROS accumulation. Collectively, our findings demonstrated that NC-T5–5TR1 enhanced the radiosensitivity of TNBC cells, and this effect was associated with the modulation of the IL-6/JAK2/STAT3 axis, offering a promising strategy for improving the therapeutic efficacy of radiotherapy in TNBC.
{"title":"Enhancing radiosensitization in triple-negative breast cancer by aptamer-modified nanoclusters NC-T5-5TR1","authors":"Xuechun Kan , Jifei Wang , Yan Li , Dongdong Li , Fan Li , Cheng Huang , Peidang Liu","doi":"10.1016/j.colsurfb.2025.115397","DOIUrl":"10.1016/j.colsurfb.2025.115397","url":null,"abstract":"<div><div>Radiotherapy is an important part of standard treatment for triple-negative breast cancer (TNBC); however, radioresistance significantly limits therapeutic outcomes. In this study, we developed a novel radiosensitizer based on aptamer-modified silver nanoclusters, NC-T5–5TR1, to potentiate radiotherapy efficacy against TNBC. NC-T5–5TR1 enabled real-time tumor imaging and accurate localization via their intrinsic fluorescence and targeting capability. When combined with ionizing radiation (IR), NC-T5–5TR1 significantly enhanced radiosensitization <em>in vitro</em>, as evidenced by decreased proliferation, increased apoptosis and higher reactive oxygen species (ROS) levels, and also markedly inhibited tumor growth <em>in vivo</em>. Transcriptomic analysis indicated that the IL-6/JAK2/STAT3 signaling pathway was downregulated under NC-T5–5TR1 + IR treatment, which was subsequently validated <em>in vitro</em> and <em>in vivo</em>. Rescue experiments confirmed that IL-6 overexpression reversed NC-T5–5TR1 combined with IR-induced JAK2/STAT3 inhibition and its downstream effects on proliferation, apoptosis, and ROS accumulation. Collectively, our findings demonstrated that NC-T5–5TR1 enhanced the radiosensitivity of TNBC cells, and this effect was associated with the modulation of the IL-6/JAK2/STAT3 axis, offering a promising strategy for improving the therapeutic efficacy of radiotherapy in TNBC.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"260 ","pages":"Article 115397"},"PeriodicalIF":5.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145838043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-04-01Epub Date: 2026-01-02DOI: 10.1016/j.colsurfb.2026.115413
Huan Luo , Shoubei Gao , Chenran Zhen , Haiyan Xiao , Yanshuai Cui , Longgang Wang
Ascorbic acid (AA), an essential water-soluble antioxidant in humans, plays a critical role in disease prevention and health maintenance, necessitating accurate detection methods. However, conventional H2O2-dependent colorimetric methods face inherent limitations, creating a demand for alternative detection strategies that operate independently of H2O2. To address this problem, a composite nanozyme (PCN-224-PDA-PdPt3) was constructed by employing a zirconium-based porphyrin metal-organic framework (PCN-224) as a substrate, followed by sequential polydopamine (PDA) surface functionalization and PdPt3 bimetallic nanoparticle deposition. The synthetic nanozyme exhibited exceptional oxidase-mimicking activity at pH 4.0 and 40 °C. Mechanism studies confirmed that its activity originates from the catalytic generation of reactive oxygen species (O2•⁻ and 1O2) from dissolved oxygen. Leveraging this, an H2O2-independent colorimetric AA detection method was developed, which demonstrated a wide linear range (1–900 μM), a low detection limit (0.56 μM), and high selectivity against interfering species like amino acids and metal ions. The clinical applicability was further verified through satisfactory recoveries (96.84–104.46 %) and low RSD (< 5 %) in spiked human serum samples. Moreover, the nanozyme showed excellent biocompatibility with a hemolysis rate below 5 %. This work not only provides a robust and safe platform for biomedical detection but also paves the way for the rational design of multifunctional nanozymes for developing H₂O₂-independent sensing systems.
{"title":"Polydopamine-functionalized PCN-224-supported Pt-Pd bimetallic nanozyme with intrinsic oxidase activity for hydrogen peroxide-free ultrasensitive colorimetric sensing of ascorbic acid","authors":"Huan Luo , Shoubei Gao , Chenran Zhen , Haiyan Xiao , Yanshuai Cui , Longgang Wang","doi":"10.1016/j.colsurfb.2026.115413","DOIUrl":"10.1016/j.colsurfb.2026.115413","url":null,"abstract":"<div><div>Ascorbic acid (AA), an essential water-soluble antioxidant in humans, plays a critical role in disease prevention and health maintenance, necessitating accurate detection methods. However, conventional H<sub>2</sub>O<sub>2</sub>-dependent colorimetric methods face inherent limitations, creating a demand for alternative detection strategies that operate independently of H<sub>2</sub>O<sub>2</sub>. To address this problem, a composite nanozyme (PCN-224-PDA-PdPt<sub>3</sub>) was constructed by employing a zirconium-based porphyrin metal-organic framework (PCN-224) as a substrate, followed by sequential polydopamine (PDA) surface functionalization and PdPt<sub>3</sub> bimetallic nanoparticle deposition. The synthetic nanozyme exhibited exceptional oxidase-mimicking activity at pH 4.0 and 40 °C. Mechanism studies confirmed that its activity originates from the catalytic generation of reactive oxygen species (O<sub>2</sub>•⁻ and <sup>1</sup>O<sub>2</sub>) from dissolved oxygen. Leveraging this, an H<sub>2</sub>O<sub>2</sub>-independent colorimetric AA detection method was developed, which demonstrated a wide linear range (1–900 μM), a low detection limit (0.56 μM), and high selectivity against interfering species like amino acids and metal ions. The clinical applicability was further verified through satisfactory recoveries (96.84–104.46 %) and low RSD (< 5 %) in spiked human serum samples. Moreover, the nanozyme showed excellent biocompatibility with a hemolysis rate below 5 %. This work not only provides a robust and safe platform for biomedical detection but also paves the way for the rational design of multifunctional nanozymes for developing H₂O₂-independent sensing systems.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"260 ","pages":"Article 115413"},"PeriodicalIF":5.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145931740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
To explore the fundamental properties and biomedical potential of Superparamagnetic Iron Oxide Nanoparticles (SPIONs), a multidisciplinary approach involving chemists, physicists, and material scientists is essential. This review highlights the unique magnetic properties of SPIONs compared to bulk magnetic materials, alongside their synthesis, characterization methods, and the factors influencing their magnetic behavior. We delve into the diverse biomedical applications of SPIONs, including their roles as contrast agents in imaging, drug carriers, and therapeutic agents for cancer treatment. Special attention is given to the concept of theranostics, where SPIONs are utilized for both therapy and diagnosis within a single system. We comprehensively summarize recent advances in the use of SPIONs for cancer theranostics over the past decade while briefly addressing their toxicity and biodegradability. Finally, the review discusses the current challenges and future prospects of SPIONs in theranostic applications including its explorations in ongoing clinical trials. The current study addresses the challenges and future perspectives in applying SPIONs in oncology, providing a roadmap for future research and development. Furthermore, considering the potential of SPION-based therapeutic platforms to achieve targeted anticancer efficacy, this article is expected to make a significant contribution to the existing body of knowledge and inspire further advancements in this rapidly evolving field.
{"title":"Transformative progress of SPIONs in cancer theranostics: A comprehensive review on recent advances in SPIONs technology","authors":"Gourang Hari Gupta , Khyati Parmar , Sumanta Ghosh , Simran Gupta , Vijay Aherwar , Shreya Kadam , Saichand Thakkellapati , Mounika Choppadandi , K. Srinivasa Rao , Sarath Babu Srivalliputtur , Govinda Kapusetti","doi":"10.1016/j.colsurfb.2025.115372","DOIUrl":"10.1016/j.colsurfb.2025.115372","url":null,"abstract":"<div><div>To explore the fundamental properties and biomedical potential of Superparamagnetic Iron Oxide Nanoparticles (SPIONs), a multidisciplinary approach involving chemists, physicists, and material scientists is essential. This review highlights the unique magnetic properties of SPIONs compared to bulk magnetic materials, alongside their synthesis, characterization methods, and the factors influencing their magnetic behavior. We delve into the diverse biomedical applications of SPIONs, including their roles as contrast agents in imaging, drug carriers, and therapeutic agents for cancer treatment. Special attention is given to the concept of theranostics, where SPIONs are utilized for both therapy and diagnosis within a single system. We comprehensively summarize recent advances in the use of SPIONs for cancer theranostics over the past decade while briefly addressing their toxicity and biodegradability. Finally, the review discusses the current challenges and future prospects of SPIONs in theranostic applications including its explorations in ongoing clinical trials. The current study addresses the challenges and future perspectives in applying SPIONs in oncology, providing a roadmap for future research and development. Furthermore, considering the potential of SPION-based therapeutic platforms to achieve targeted anticancer efficacy, this article is expected to make a significant contribution to the existing body of knowledge and inspire further advancements in this rapidly evolving field.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"260 ","pages":"Article 115372"},"PeriodicalIF":5.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145779748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
扫码关注我们
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号