首页 > 最新文献

ACS Measurement Science Au最新文献

英文 中文
Abiotic, Hybrid, and Biological Electrocatalytic Materials Applied in Microfluidic Fuel Cells: A Comprehensive Review 应用于微流控燃料电池的非生物、混合和生物电催化材料:全面综述
Q1 CHEMISTRY, ANALYTICAL Pub Date : 2023-11-06 DOI: 10.1021/acsmeasuresciau.3c00044
D. V. Estrada-Osorio, Ricardo A. Escalona-Villalpando, M. P. Gurrola, Ricardo Chaparro-Sánchez, J. A. Rodríguez-Morales, L. G. Arriaga and J. Ledesma-García*, 

This article provides an overview of the work reported in the past decade in the field of microfluidic fuel cells. To develop appropriate research, the most commonly used electrocatalytic materials were considered and a new classification was proposed based on their nature: abiotic, hybrid, or biological. This classification allowed the authors to discern the information collected. In this sense, the types of electrocatalysts used for the oxidation of the most common fuels in different environments, such as glucose, ethanol, methanol, glycerol, and lactate, were presented. There are several phenomena presented in this article. This information gives an overview of where research is heading in the field of materials for electrocatalysis, regardless of the fuel used in the microfluidic fuel cell: the synthesis of abiotic and biological materials to obtain hybrid materials that allow the use of the best properties of each material.

本文概述了过去十年中微流体燃料电池领域的研究成果。为了开展适当的研究,作者考虑了最常用的电催化材料,并根据其性质提出了新的分类方法:非生物、混合或生物。通过这种分类,作者可以对收集到的信息进行鉴别。从这个意义上说,作者介绍了在不同环境中用于氧化葡萄糖、乙醇、甲醇、甘油和乳酸盐等最常见燃料的电催化剂类型。本文介绍了几种现象。这些信息概述了电催化材料领域的研究方向,无论微流体燃料电池使用何种燃料:合成非生物和生物材料,以获得混合材料,从而利用每种材料的最佳特性。
{"title":"Abiotic, Hybrid, and Biological Electrocatalytic Materials Applied in Microfluidic Fuel Cells: A Comprehensive Review","authors":"D. V. Estrada-Osorio,&nbsp;Ricardo A. Escalona-Villalpando,&nbsp;M. P. Gurrola,&nbsp;Ricardo Chaparro-Sánchez,&nbsp;J. A. Rodríguez-Morales,&nbsp;L. G. Arriaga and J. Ledesma-García*,&nbsp;","doi":"10.1021/acsmeasuresciau.3c00044","DOIUrl":"10.1021/acsmeasuresciau.3c00044","url":null,"abstract":"<p >This article provides an overview of the work reported in the past decade in the field of microfluidic fuel cells. To develop appropriate research, the most commonly used electrocatalytic materials were considered and a new classification was proposed based on their nature: abiotic, hybrid, or biological. This classification allowed the authors to discern the information collected. In this sense, the types of electrocatalysts used for the oxidation of the most common fuels in different environments, such as glucose, ethanol, methanol, glycerol, and lactate, were presented. There are several phenomena presented in this article. This information gives an overview of where research is heading in the field of materials for electrocatalysis, regardless of the fuel used in the microfluidic fuel cell: the synthesis of abiotic and biological materials to obtain hybrid materials that allow the use of the best properties of each material.</p>","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"4 1","pages":"25–41"},"PeriodicalIF":0.0,"publicationDate":"2023-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsmeasuresciau.3c00044","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135634304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Nanosensor Chemical Cytometry: Advances and Opportunities in Cellular Therapy and Precision Medicine 纳米传感器化学细胞测量法:细胞疗法和精准医学的进步与机遇
Q1 CHEMISTRY, ANALYTICAL Pub Date : 2023-11-01 DOI: 10.1021/acsmeasuresciau.3c00038
Youngho Song, Changyu Tian, Yullim Lee, Minyeong Yoon, Sang Eun Yoon and Soo-Yeon Cho*, 

With the definition of therapeutics now encompassing transplanted or engineered cells and their molecular products, there is a growing scientific necessity for analytics to understand this new category of drugs. This Perspective highlights the recent development of new measurement science on label-free single cell analysis, nanosensor chemical cytometry (NCC), and their potential for cellular therapeutics and precision medicine. NCC is based on microfluidics integrated with fluorescent nanosensor arrays utilizing the optical lensing effect of a single cell to real-time extract molecular properties and correlate them with physical attributes of single cells. This new class of cytometry can quantify the heterogeneity of the multivariate physicochemical attributes of the cell populations in a completely label-free and nondestructive way and, thus, suggest the vein-to-vein conditions for the safe therapeutic applications. After the introduction of the NCC technology, we suggest the technological development roadmap for the maturation of the new field: from the sensor/chip design perspective to the system/software development level based on hardware automation and deep learning data analytics. The advancement of this new single cell sensing technology is anticipated to aid rich and multivariate single cell data setting and support safe and reliable cellular therapeutics. This new measurement science can lead to data-driven personalized precision medicine.

随着治疗学的定义已涵盖移植或工程细胞及其分子产物,科学界越来越需要通过分析来了解这一新的药物类别。本视角重点介绍无标记单细胞分析、纳米传感器化学细胞仪(NCC)等新型测量科学的最新发展及其在细胞疗法和精准医疗方面的潜力。NCC 基于集成了荧光纳米传感器阵列的微流体技术,利用单细胞的光学透镜效应实时提取分子特性,并将其与单细胞的物理属性相关联。这种新型细胞测量技术可以完全无标记和无损的方式量化细胞群的多元物理化学属性的异质性,从而为安全的治疗应用提供静脉到静脉条件的建议。在介绍了 NCC 技术之后,我们为新领域的成熟提出了技术发展路线图:从传感器/芯片设计角度到基于硬件自动化和深度学习数据分析的系统/软件开发层面。预计这种新型单细胞传感技术的进步将有助于丰富的多变量单细胞数据设置,并为安全可靠的细胞疗法提供支持。这一新的测量科学可实现数据驱动的个性化精准医疗。
{"title":"Nanosensor Chemical Cytometry: Advances and Opportunities in Cellular Therapy and Precision Medicine","authors":"Youngho Song,&nbsp;Changyu Tian,&nbsp;Yullim Lee,&nbsp;Minyeong Yoon,&nbsp;Sang Eun Yoon and Soo-Yeon Cho*,&nbsp;","doi":"10.1021/acsmeasuresciau.3c00038","DOIUrl":"10.1021/acsmeasuresciau.3c00038","url":null,"abstract":"<p >With the definition of therapeutics now encompassing transplanted or engineered cells and their molecular products, there is a growing scientific necessity for analytics to understand this new category of drugs. This Perspective highlights the recent development of new measurement science on label-free single cell analysis, nanosensor chemical cytometry (NCC), and their potential for cellular therapeutics and precision medicine. NCC is based on microfluidics integrated with fluorescent nanosensor arrays utilizing the optical lensing effect of a single cell to real-time extract molecular properties and correlate them with physical attributes of single cells. This new class of cytometry can quantify the heterogeneity of the multivariate physicochemical attributes of the cell populations in a completely label-free and nondestructive way and, thus, suggest the vein-to-vein conditions for the safe therapeutic applications. After the introduction of the NCC technology, we suggest the technological development roadmap for the maturation of the new field: from the sensor/chip design perspective to the system/software development level based on hardware automation and deep learning data analytics. The advancement of this new single cell sensing technology is anticipated to aid rich and multivariate single cell data setting and support safe and reliable cellular therapeutics. This new measurement science can lead to data-driven personalized precision medicine.</p>","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"3 6","pages":"393–403"},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsmeasuresciau.3c00038","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135325564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Aiming for Maximized and Reproducible Enhancements in the Obstacle Race of SERS 在 SERS 的障碍赛中追求最大化和可重复的增强效果
Q1 CHEMISTRY, ANALYTICAL Pub Date : 2023-10-31 DOI: 10.1021/acsmeasuresciau.3c00037
Priyanka Dey*, 

Surface enhanced Raman scattering (SERS), since its discovery in the mid-1970s, has taken on many roles in the world of analytical measurement science. From identifying known and unknown chemicals in mixtures such as pharmaceutical and environmental samples to enabling qualitative and quantitative analysis of biomolecules and biomedical disease markers (or biomarkers), furthermore expanding to tracking nanostructures in vivo for medical diagnosis and therapy. This is because SERS combines the inherent power of Raman scattering capable of molecular species identification, topped with tremendous amplification in the Raman signal intensity when the molecule of interest is positioned near plasmonic nanostructures. The higher the SERS signal amplification, the lower the limit of detection (LOD) that could be achieved for the above applications. Therefore, improving SERS sensing efficiencies is vital. The signal reproducibility and SERS enhancement factor (EF) heavily rely on plasmonic nanostructure design, which has led to tremendous work in the field. But SERS signal and EF reproducibility remain key limitations for its wider market usability. This Review will scrutinize factors, some recognized and some often overlooked, that dictate the SERS signal and are of utmost importance to enable reproducible SERS EFs. Most of the factors pertain to colloidal labeled SERS. Some critically reviewed factors include the nanostructure’s surface area as a limiting factor, SERS hot-spots including optimizing the SERS EF within the hot-spot volume and positioning labels, properties of label molecules governing molecule orientation in hot-spots, and resonance effects. A better understanding of these factors will enable improved optimization and control of the experimental SERS, enabling extremely sensitive LODs without overestimating the SERS EFs. These are crucial steps toward identification and reproducible quantification in SERS sensing.

自 20 世纪 70 年代中期发现表面增强拉曼散射(SERS)以来,它在分析测量科学领域发挥了许多作用。从识别医药和环境样品等混合物中的已知和未知化学物质,到对生物大分子和生物医学疾病标志物(或生物标记物)进行定性和定量分析,再到追踪体内纳米结构以进行医学诊断和治疗。这是因为 SERS 结合了拉曼散射固有的分子物种识别能力,当感兴趣的分子靠近等离子纳米结构时,拉曼信号强度会被极大放大。SERS 信号放大率越高,上述应用所能达到的检测限(LOD)就越低。因此,提高 SERS 传感效率至关重要。信号重现性和 SERS 增强因子(EF)在很大程度上依赖于等离子纳米结构的设计,这也导致了该领域的大量研究工作。但是,SERS 信号和 EF 可重复性仍然是其广泛市场应用的关键限制因素。本综述将仔细研究决定 SERS 信号的因素,其中有些是公认的,有些则经常被忽视,而这些因素对于实现 SERS EF 的可重复性至关重要。大多数因素都与胶体标记 SERS 有关。一些重要因素包括作为限制因素的纳米结构表面积、SERS 热点(包括优化热点体积内的 SERS EF 和定位标签)、标签分子在热点中的取向特性以及共振效应。更好地了解这些因素将有助于改进对 SERS 实验的优化和控制,从而在不高估 SERS EF 的情况下实现极其灵敏的 LOD。这些都是在 SERS 传感中实现识别和可重复量化的关键步骤。
{"title":"Aiming for Maximized and Reproducible Enhancements in the Obstacle Race of SERS","authors":"Priyanka Dey*,&nbsp;","doi":"10.1021/acsmeasuresciau.3c00037","DOIUrl":"10.1021/acsmeasuresciau.3c00037","url":null,"abstract":"<p >Surface enhanced Raman scattering (SERS), since its discovery in the mid-1970s, has taken on many roles in the world of analytical measurement science. From identifying known and unknown chemicals in mixtures such as pharmaceutical and environmental samples to enabling qualitative and quantitative analysis of biomolecules and biomedical disease markers (or biomarkers), furthermore expanding to tracking nanostructures in vivo for medical diagnosis and therapy. This is because SERS combines the inherent power of Raman scattering capable of molecular species identification, topped with tremendous amplification in the Raman signal intensity when the molecule of interest is positioned near plasmonic nanostructures. The higher the SERS signal amplification, the lower the limit of detection (LOD) that could be achieved for the above applications. Therefore, improving SERS sensing efficiencies is vital. The signal reproducibility and SERS enhancement factor (EF) heavily rely on plasmonic nanostructure design, which has led to tremendous work in the field. But SERS signal and EF reproducibility remain key limitations for its wider market usability. This Review will scrutinize factors, some recognized and some often overlooked, that dictate the SERS signal and are of utmost importance to enable reproducible SERS EFs. Most of the factors pertain to colloidal labeled SERS. Some critically reviewed factors include the nanostructure’s surface area as a limiting factor, SERS hot-spots including optimizing the SERS EF within the hot-spot volume and positioning labels, properties of label molecules governing molecule orientation in hot-spots, and resonance effects. A better understanding of these factors will enable improved optimization and control of the experimental SERS, enabling extremely sensitive LODs without overestimating the SERS EFs. These are crucial steps toward identification and reproducible quantification in SERS sensing.</p>","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"3 6","pages":"434–443"},"PeriodicalIF":0.0,"publicationDate":"2023-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsmeasuresciau.3c00037","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135868722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Advances in Bioelectrode Design for Developing Electrochemical Biosensors 开发电化学生物传感器的生物电极设计进展
Q1 CHEMISTRY, ANALYTICAL Pub Date : 2023-10-27 DOI: 10.1021/acsmeasuresciau.3c00034
Nabajyoti Kalita, Sudarshan Gogoi, Shelley D. Minteer* and Pranab Goswami*, 

The critical performance factors such as selectivity, sensitivity, operational and storage stability, and response time of electrochemical biosensors are governed mainly by the function of their key component, the bioelectrode. Suitable design and fabrication strategies of the bioelectrode interface are essential for realizing the requisite performance of the biosensors for their practical utility. A multifaceted attempt to achieve this goal is visible from the vast literature exploring effective strategies for preparing, immobilizing, and stabilizing biorecognition elements on the electrode surface and efficient transduction of biochemical signals into electrical ones (i.e., current, voltage, and impedance) through the bioelectrode interface with the aid of advanced materials and techniques. The commercial success of biosensors in modern society is also increasingly influenced by their size (and hence portability), multiplexing capability, and coupling in the interface of the wireless communication technology, which facilitates quick data transfer and linked decision-making processes in real-time in different areas such as healthcare, agriculture, food, and environmental applications. Therefore, fabrication of the bioelectrode involves careful selection and control of several parameters, including biorecognition elements, electrode materials, shape and size of the electrode, detection principles, and various fabrication strategies, including microscale and printing technologies. This review discusses recent trends in bioelectrode designs and fabrications for developing electrochemical biosensors. The discussions have been delineated into the types of biorecognition elements and their immobilization strategies, signal transduction approaches, commonly used advanced materials for electrode fabrication and techniques for fabricating the bioelectrodes, and device integration with modern electronic communication technology for developing electrochemical biosensors of commercial interest.

电化学生物传感器的选择性、灵敏度、操作和存储稳定性以及响应时间等关键性能因素主要受其关键部件生物电极功能的影响。生物电极界面的适当设计和制造策略对于实现生物传感器的必要性能和实际用途至关重要。为实现这一目标,人们进行了多方面的尝试,从大量文献中可以看出,这些文献探讨了在电极表面制备、固定和稳定生物识别元件的有效策略,并借助先进的材料和技术,通过生物电极界面将生化信号有效地转化为电信号(即电流、电压和阻抗)。生物传感器在现代社会中的商业成功也日益受到其尺寸(因此便携性)、多路复用能力和无线通信技术接口耦合的影响,无线通信技术有利于在医疗保健、农业、食品和环境应用等不同领域快速传输数据和实时联动决策过程。因此,生物电极的制造涉及对多个参数的精心选择和控制,包括生物识别元件、电极材料、电极形状和尺寸、检测原理以及各种制造策略,包括微尺度和打印技术。本综述讨论了用于开发电化学生物传感器的生物电极设计和制造的最新趋势。讨论内容包括生物识别元件的类型及其固定策略、信号转导方法、电极制造常用的先进材料和生物电极制造技术,以及为开发具有商业价值的电化学生物传感器而将设备与现代电子通信技术相结合。
{"title":"Advances in Bioelectrode Design for Developing Electrochemical Biosensors","authors":"Nabajyoti Kalita,&nbsp;Sudarshan Gogoi,&nbsp;Shelley D. Minteer* and Pranab Goswami*,&nbsp;","doi":"10.1021/acsmeasuresciau.3c00034","DOIUrl":"10.1021/acsmeasuresciau.3c00034","url":null,"abstract":"<p >The critical performance factors such as selectivity, sensitivity, operational and storage stability, and response time of electrochemical biosensors are governed mainly by the function of their key component, the bioelectrode. Suitable design and fabrication strategies of the bioelectrode interface are essential for realizing the requisite performance of the biosensors for their practical utility. A multifaceted attempt to achieve this goal is visible from the vast literature exploring effective strategies for preparing, immobilizing, and stabilizing biorecognition elements on the electrode surface and efficient transduction of biochemical signals into electrical ones (i.e., current, voltage, and impedance) through the bioelectrode interface with the aid of advanced materials and techniques. The commercial success of biosensors in modern society is also increasingly influenced by their size (and hence portability), multiplexing capability, and coupling in the interface of the wireless communication technology, which facilitates quick data transfer and linked decision-making processes in real-time in different areas such as healthcare, agriculture, food, and environmental applications. Therefore, fabrication of the bioelectrode involves careful selection and control of several parameters, including biorecognition elements, electrode materials, shape and size of the electrode, detection principles, and various fabrication strategies, including microscale and printing technologies. This review discusses recent trends in bioelectrode designs and fabrications for developing electrochemical biosensors. The discussions have been delineated into the types of biorecognition elements and their immobilization strategies, signal transduction approaches, commonly used advanced materials for electrode fabrication and techniques for fabricating the bioelectrodes, and device integration with modern electronic communication technology for developing electrochemical biosensors of commercial interest.</p>","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"3 6","pages":"404–433"},"PeriodicalIF":0.0,"publicationDate":"2023-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsmeasuresciau.3c00034","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136318293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Analyzer-to-Analyzer Variations in Assaying Ultralow Concentrated Biomarkers Associated with Neurodegenerative Diseases Using Immunomagnetic Reduction 利用免疫磁性还原法检测与神经退行性疾病相关的超低浓度生物标记物时分析仪之间的差异
Q1 CHEMISTRY, ANALYTICAL Pub Date : 2023-10-23 DOI: 10.1021/acsmeasuresciau.3c00029
Kun-Hung Lee, Ming-Hung Hsu, Hsin-Hsien Chen and Shieh-Yueh Yang*, 

By utilizing a high-temperature superconducting quantum interference device (high-Tc SQUID) magnetometer, an alternating current (AC) magnetosusceptometer, referred to as an analyzer, was developed for ultrasensitive immunoassays. The analyzer has been applied to assay biomarkers in human plasma associated with Alzheimer’s disease (AD) and Parkinson’s disease (PD). The involved assay methodology is the so-called immunomagnetic reduction (IMR). Such an analyzer has been approved for clinical use in Taiwan and Europe. The mass production of the analyzer is needed for clinical utilities. The issue of exploring analyzer-to-analyzer variations in the performances becomes critical. Unfortunately, there is no standard characterization to determine the variations in performances among analyzers. In this study, key characterizations, such as output signal stability, signal-to-noise ratio, measured concentrations of a control sample, etc., are proposed. In total, three analyzers are characterized in this work. The detected biomarkers include amyloid peptides, total tau protein, phosphorylated tau protein, and α-synuclein protein for AD and PD. Through one-way ANOVA for any of the characterizations among the three analyzers, it was found that there was no significant difference in any of these characterizations among the analyzers (p > 0.05). Furthermore, the three analyzers are applied to assay biomolecules for AD and PD in reference samples. High correlations (r > 0.8) in measured concentrations of any of these biomarkers in reference samples were obtained among the three analyzers. The results demonstrate that the proposed characterizations are feasible for achieving consistent performance among high-Tc SQUID-based AC magnetosusceptometers for assaying biomolecules.

通过利用高温超导量子干涉装置(high-Tc SQUID)磁力计,开发出了一种用于超灵敏免疫测定的交流(AC)磁感测器,称为分析仪。该分析仪已被用于检测人体血浆中与阿尔茨海默病(AD)和帕金森病(PD)相关的生物标记物。其中涉及的检测方法是所谓的免疫磁还原法(IMR)。这种分析仪已在台湾和欧洲获准用于临床。临床应用需要大量生产这种分析仪。探索分析仪之间的性能差异成为关键问题。遗憾的是,目前还没有标准的表征方法来确定分析仪之间的性能差异。在本研究中,我们提出了一些关键特性,如输出信号稳定性、信噪比、对照样本的测量浓度等。本研究共对三种分析仪进行了表征。检测的生物标记物包括淀粉样肽、总tau蛋白、磷酸化tau蛋白和α-突触核蛋白(AD和PD)。通过对三种分析仪的任何特征进行单因素方差分析,发现分析仪之间的任何特征均无显著差异(p > 0.05)。此外,这三种分析仪还被用于检测参考样本中的AD和PD生物大分子。三台分析仪在参考样本中测得的这些生物标记物的浓度具有很高的相关性(r > 0.8)。结果表明,所提出的特征描述是可行的,可以使基于高锝 SQUID 的交流磁差计在检测生物大分子时获得一致的性能。
{"title":"Analyzer-to-Analyzer Variations in Assaying Ultralow Concentrated Biomarkers Associated with Neurodegenerative Diseases Using Immunomagnetic Reduction","authors":"Kun-Hung Lee,&nbsp;Ming-Hung Hsu,&nbsp;Hsin-Hsien Chen and Shieh-Yueh Yang*,&nbsp;","doi":"10.1021/acsmeasuresciau.3c00029","DOIUrl":"10.1021/acsmeasuresciau.3c00029","url":null,"abstract":"<p >By utilizing a high-temperature superconducting quantum interference device (high-<i>T</i><sub>c</sub> SQUID) magnetometer, an alternating current (AC) magnetosusceptometer, referred to as an analyzer, was developed for ultrasensitive immunoassays. The analyzer has been applied to assay biomarkers in human plasma associated with Alzheimer’s disease (AD) and Parkinson’s disease (PD). The involved assay methodology is the so-called immunomagnetic reduction (IMR). Such an analyzer has been approved for clinical use in Taiwan and Europe. The mass production of the analyzer is needed for clinical utilities. The issue of exploring analyzer-to-analyzer variations in the performances becomes critical. Unfortunately, there is no standard characterization to determine the variations in performances among analyzers. In this study, key characterizations, such as output signal stability, signal-to-noise ratio, measured concentrations of a control sample, etc., are proposed. In total, three analyzers are characterized in this work. The detected biomarkers include amyloid peptides, total tau protein, phosphorylated tau protein, and α-synuclein protein for AD and PD. Through one-way ANOVA for any of the characterizations among the three analyzers, it was found that there was no significant difference in any of these characterizations among the analyzers (<i>p</i> &gt; 0.05). Furthermore, the three analyzers are applied to assay biomolecules for AD and PD in reference samples. High correlations (<i>r</i> &gt; 0.8) in measured concentrations of any of these biomarkers in reference samples were obtained among the three analyzers. The results demonstrate that the proposed characterizations are feasible for achieving consistent performance among high-<i>T</i><sub>c</sub> SQUID-based AC magnetosusceptometers for assaying biomolecules.</p>","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"3 6","pages":"488–495"},"PeriodicalIF":0.0,"publicationDate":"2023-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsmeasuresciau.3c00029","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135366559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Spatial Proteomics toward Subcellular Resolution by Coupling Deep Ultraviolet Laser Ablation with Nanodroplet Sample Preparation 将深紫外激光烧蚀与纳米液滴样品制备结合起来进行空间蛋白质组学研究,提高亚细胞分辨率
Q1 CHEMISTRY, ANALYTICAL Pub Date : 2023-10-20 DOI: 10.1021/acsmeasuresciau.3c00033
Piliang Xiang, Andrey Liyu, Yumi Kwon, Dehong Hu, Sarah M. Williams, Dušan Veličković, Lye Meng Markillie, William B. Chrisler, Ljiljana Paša-Tolić* and Ying Zhu*, 

Multiplexed molecular profiling of tissue microenvironments, or spatial omics, can provide critical insights into cellular functions and disease pathology. The coupling of laser microdissection with mass spectrometry-based proteomics has enabled deep and unbiased mapping of >1000 proteins. However, the throughput of laser microdissection is often limited due to tedious two-step procedures, sequential laser cutting, and sample collection. The two-step procedure also hinders the further improvement of spatial resolution to <10 μm as needed for subcellular proteomics. Herein, we developed a high-throughput and high-resolution spatial proteomics platform by seamlessly coupling deep ultraviolet (DUV) laser ablation (LA) with nanoPOTS (Nanodroplet Processing in One pot for Trace Samples)-based sample preparation. We demonstrated the DUV-LA system can quickly isolate and collect tissue samples at a throughput of ∼30 spots/min and a spatial resolution down to 2 μm from a 10 μm thick human pancreas tissue section. To improve sample recovery, we developed a proximity aerosol collection approach by placing DMSO droplets close to LA spots. We demonstrated the DUV-LA-nanoPOTS platform can detect an average of 1312, 1533, and 1966 proteins from ablation spots with diameters of 7, 13, and 19 μm, respectively. In a proof-of-concept study, we isolated and profiled two distinct subcellular regions of the pancreas tissue revealed by hematoxylin and eosin (H&E) staining. Quantitative proteomics revealed proteins specifically enriched to subcellular compartments.

组织微环境的多重分子图谱分析(或称空间 omics)可提供有关细胞功能和疾病病理的重要见解。将激光显微切割与基于质谱的蛋白质组学相结合,可以对 1000 种蛋白质进行深入而无偏见的绘制。然而,激光显微切割的通量往往受到繁琐的两步程序、连续激光切割和样品采集的限制。两步程序还阻碍了亚细胞蛋白质组学所需的空间分辨率进一步提高到 10 μm。在此,我们开发了一种高通量、高分辨率的空间蛋白质组学平台,将深紫外(DUV)激光烧蚀(LA)与基于样品制备的纳米POTS(痕量样品的纳米液滴一次性处理)无缝结合。我们展示了 DUV-LA 系统能以每分钟 ∼30 个点的吞吐量和低至 2 μm 的空间分辨率从 10 μm 厚的人体胰腺组织切片中快速分离和采集组织样本。为了提高样品回收率,我们开发了一种近距离气溶胶收集方法,将 DMSO 液滴放置在靠近 LA 点的位置。我们证明,DUV-LA-nanoPOTS 平台能从直径分别为 7、13 和 19 μm 的烧蚀点平均检测到 1312、1533 和 1966 个蛋白质。在概念验证研究中,我们分离并分析了苏木精和伊红(H&E)染色显示的胰腺组织的两个不同亚细胞区域。定量蛋白质组学揭示了特异性富集于亚细胞区的蛋白质。
{"title":"Spatial Proteomics toward Subcellular Resolution by Coupling Deep Ultraviolet Laser Ablation with Nanodroplet Sample Preparation","authors":"Piliang Xiang,&nbsp;Andrey Liyu,&nbsp;Yumi Kwon,&nbsp;Dehong Hu,&nbsp;Sarah M. Williams,&nbsp;Dušan Veličković,&nbsp;Lye Meng Markillie,&nbsp;William B. Chrisler,&nbsp;Ljiljana Paša-Tolić* and Ying Zhu*,&nbsp;","doi":"10.1021/acsmeasuresciau.3c00033","DOIUrl":"10.1021/acsmeasuresciau.3c00033","url":null,"abstract":"<p >Multiplexed molecular profiling of tissue microenvironments, or spatial omics, can provide critical insights into cellular functions and disease pathology. The coupling of laser microdissection with mass spectrometry-based proteomics has enabled deep and unbiased mapping of &gt;1000 proteins. However, the throughput of laser microdissection is often limited due to tedious two-step procedures, sequential laser cutting, and sample collection. The two-step procedure also hinders the further improvement of spatial resolution to &lt;10 μm as needed for subcellular proteomics. Herein, we developed a high-throughput and high-resolution spatial proteomics platform by seamlessly coupling deep ultraviolet (DUV) laser ablation (LA) with nanoPOTS (Nanodroplet Processing in One pot for Trace Samples)-based sample preparation. We demonstrated the DUV-LA system can quickly isolate and collect tissue samples at a throughput of ∼30 spots/min and a spatial resolution down to 2 μm from a 10 μm thick human pancreas tissue section. To improve sample recovery, we developed a proximity aerosol collection approach by placing DMSO droplets close to LA spots. We demonstrated the DUV-LA-nanoPOTS platform can detect an average of 1312, 1533, and 1966 proteins from ablation spots with diameters of 7, 13, and 19 μm, respectively. In a proof-of-concept study, we isolated and profiled two distinct subcellular regions of the pancreas tissue revealed by hematoxylin and eosin (H&amp;E) staining. Quantitative proteomics revealed proteins specifically enriched to subcellular compartments.</p>","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"3 6","pages":"459–468"},"PeriodicalIF":0.0,"publicationDate":"2023-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsmeasuresciau.3c00033","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135570253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Luciferase Calibrants Enable Absolute Quantitation of Bioluminescence Power 荧光素酶校准物可实现生物发光功率的绝对定量
Q1 CHEMISTRY, ANALYTICAL Pub Date : 2023-10-20 DOI: 10.1021/acsmeasuresciau.3c00036
Mark A. Klein*, Sergey Lazarev, Charles Gervasi, Cristopher Cowan, Thomas Machleidt and Rachel Friedman Ohana*, 

Bioluminescence emitted from a luciferase-catalyzed oxidation of luciferin has been broadly utilized to report on biological events, predominantly through relative changes in the light output. Recent advances in protein engineering and synthetic chemistry have yielded bioluminescent systems with markedly improved brightness and bioavailability. These developments have enabled not only the detection of biological events at far lower expression levels but also new opportunities utilizing bioluminescence to power photochemistry in cells. Regardless of the application, bioluminescence analyses have leaned heavily on the use of luminometers to measure the light output of a system. Current luminometers report the light output of a sample in relative units, limiting the ability to compare data between instruments and preventing the absolute power of a bioluminescent system from being quantified. Luminescent solution calibrants comprising luciferases and their cognate luciferins that have been characterized for absolute light output would enable calibration of any given luminometer for absolute photon counting. To this end, we have built a custom light detection apparatus and used it alongside wavelength-matched LED light sources emitting at 450 and 561 nm to characterize the absolute power of a series of NanoLuc and firefly luciferase solutions, respectively. This approach revealed that these two common luciferases produce 3.72 × 10–18 and 7.25 × 10–20 watts/molecule, respectively. Components of these luminescent solution calibrants are commercially available and produce stable bioluminescent signals over 2–5 min, enabling any luminometer to be calibrated for power measurements of bioluminescence emitted by these two luciferases in units of watts or photons per second.

由荧光素酶催化氧化荧光素而发出的生物发光已被广泛用于报告生物事件,主要是通过光输出的相对变化。蛋白质工程和合成化学领域的最新进展已经产生了亮度和生物利用率显著提高的生物发光系统。这些发展不仅使生物事件的检测表达水平大大降低,也为利用生物发光驱动细胞内的光化学提供了新的机会。无论是哪种应用,生物发光分析在很大程度上都依赖于使用光度计来测量系统的光输出。目前的光度计以相对单位报告样品的光输出,限制了仪器间数据比较的能力,也无法量化生物发光系统的绝对功率。由荧光酶及其同源荧光素组成的荧光溶液校准液具有绝对光输出的特征,可以校准任何特定的发光仪进行绝对光子计数。为此,我们定制了一种光检测仪器,并将其与波长匹配的 LED 光源(波长分别为 450 纳米和 561 纳米)一起使用,分别鉴定了一系列 NanoLuc 和萤火虫荧光素酶溶液的绝对功率。这种方法显示,这两种常见的荧光素酶产生的功率分别为 3.72 × 10-18 瓦特/分子和 7.25 × 10-20 瓦特/分子。这些发光溶液校准液的成分可在市场上买到,并能在 2-5 分钟内产生稳定的生物发光信号,因此任何发光仪都能对这两种荧光素酶发出的生物发光进行功率测量校准,单位为瓦特或光子/秒。
{"title":"Luciferase Calibrants Enable Absolute Quantitation of Bioluminescence Power","authors":"Mark A. Klein*,&nbsp;Sergey Lazarev,&nbsp;Charles Gervasi,&nbsp;Cristopher Cowan,&nbsp;Thomas Machleidt and Rachel Friedman Ohana*,&nbsp;","doi":"10.1021/acsmeasuresciau.3c00036","DOIUrl":"10.1021/acsmeasuresciau.3c00036","url":null,"abstract":"<p >Bioluminescence emitted from a luciferase-catalyzed oxidation of luciferin has been broadly utilized to report on biological events, predominantly through relative changes in the light output. Recent advances in protein engineering and synthetic chemistry have yielded bioluminescent systems with markedly improved brightness and bioavailability. These developments have enabled not only the detection of biological events at far lower expression levels but also new opportunities utilizing bioluminescence to power photochemistry in cells. Regardless of the application, bioluminescence analyses have leaned heavily on the use of luminometers to measure the light output of a system. Current luminometers report the light output of a sample in relative units, limiting the ability to compare data between instruments and preventing the absolute power of a bioluminescent system from being quantified. Luminescent solution calibrants comprising luciferases and their cognate luciferins that have been characterized for absolute light output would enable calibration of any given luminometer for absolute photon counting. To this end, we have built a custom light detection apparatus and used it alongside wavelength-matched LED light sources emitting at 450 and 561 nm to characterize the absolute power of a series of NanoLuc and firefly luciferase solutions, respectively. This approach revealed that these two common luciferases produce 3.72 × 10<sup>–18</sup> and 7.25 × 10<sup>–20</sup> watts/molecule, respectively. Components of these luminescent solution calibrants are commercially available and produce stable bioluminescent signals over 2–5 min, enabling any luminometer to be calibrated for power measurements of bioluminescence emitted by these two luciferases in units of watts or photons per second.</p>","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"3 6","pages":"496–503"},"PeriodicalIF":0.0,"publicationDate":"2023-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsmeasuresciau.3c00036","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135618067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Sensing Liquid- and Gas-Phase Hydrocarbons via Mid-Infrared Broadband Femtosecond Laser Source Spectroscopy 通过中红外宽带飞秒激光源光谱传感液相和气相碳氢化合物
Q1 CHEMISTRY, ANALYTICAL Pub Date : 2023-10-12 DOI: 10.1021/acsmeasuresciau.3c00026
Michael Hlavatsch, Andrea Teuber, Max Eisele and Boris Mizaikoff*, 

In this study, we demonstrate the combination of a tunable broadband mid-infrared (MIR) femtosecond laser source separately coupled to a ZnSe crystal horizontal attenuated total reflection (ATR) sensor cell for liquid phase samples and to a substrate-integrated hollow waveguide (iHWG) for gas phase samples. Utilizing this emerging light source technology as an alternative MIR radiation source for Fourier transform infrared (FTIR) spectroscopy opens interesting opportunities for analytical applications. In a first approach, we demonstrate the quantitative analysis of three individual samples, ethanol (liquid), methane (gas), and 2-methyl-1-propene (gas), with limits of detection of 0.3% (ethanol) and 22 ppmv and 74 ppmv (methane and isobutylene), respectively, determined at selected emission wavelengths of the MIR laser source (i.e., 890 cm–1, 1046 and 1305 cm–1). Hence, the applicability of a broadband MIR femtosecond laser source as a bright alternative light source for quantitative analysis via FTIR spectroscopy in various sensing configurations has been demonstrated.

在这项研究中,我们展示了将可调谐宽带中红外(MIR)飞秒激光光源分别与用于液相样品的 ZnSe 晶体水平衰减全反射(ATR)传感器单元和用于气相样品的基底集成空心波导(iHWG)相结合的方法。利用这种新兴光源技术作为傅立叶变换红外(FTIR)光谱的替代中红外辐射源,为分析应用带来了有趣的机遇。在第一种方法中,我们展示了对乙醇(液体)、甲烷(气体)和 2-甲基-1-丙烯(气体)这三种样品的定量分析,检测限分别为 0.3%(乙醇)、22 ppmv 和 74 ppmv(甲烷和异丁烯),是在选定的中红外激光源发射波长(即 890 cm-1、1046 和 1305 cm-1)下测定的。因此,宽带近红外飞秒激光源作为一种明亮的替代光源,适用于在各种传感配置中通过傅立叶变换红外光谱进行定量分析。
{"title":"Sensing Liquid- and Gas-Phase Hydrocarbons via Mid-Infrared Broadband Femtosecond Laser Source Spectroscopy","authors":"Michael Hlavatsch,&nbsp;Andrea Teuber,&nbsp;Max Eisele and Boris Mizaikoff*,&nbsp;","doi":"10.1021/acsmeasuresciau.3c00026","DOIUrl":"10.1021/acsmeasuresciau.3c00026","url":null,"abstract":"<p >In this study, we demonstrate the combination of a tunable broadband mid-infrared (MIR) femtosecond laser source separately coupled to a ZnSe crystal horizontal attenuated total reflection (ATR) sensor cell for liquid phase samples and to a substrate-integrated hollow waveguide (iHWG) for gas phase samples. Utilizing this emerging light source technology as an alternative MIR radiation source for Fourier transform infrared (FTIR) spectroscopy opens interesting opportunities for analytical applications. In a first approach, we demonstrate the quantitative analysis of three individual samples, ethanol (liquid), methane (gas), and 2-methyl-1-propene (gas), with limits of detection of 0.3% (ethanol) and 22 ppm<sub>v</sub> and 74 ppm<sub>v</sub> (methane and isobutylene), respectively, determined at selected emission wavelengths of the MIR laser source (i.e., 890 cm<sup>–1</sup>, 1046 and 1305 cm<sup>–1</sup>). Hence, the applicability of a broadband MIR femtosecond laser source as a bright alternative light source for quantitative analysis via FTIR spectroscopy in various sensing configurations has been demonstrated.</p>","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"3 6","pages":"452–458"},"PeriodicalIF":0.0,"publicationDate":"2023-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsmeasuresciau.3c00026","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136014499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Paper-Based Coculture Platform to Evaluate the Effects of Fibroblasts on Estrogen Signaling in ER+ Breast Cancers 评估成纤维细胞对ER+乳腺癌雌激素信号转导影响的纸基协同培养平台
Q1 CHEMISTRY, ANALYTICAL Pub Date : 2023-10-09 DOI: 10.1021/acsmeasuresciau.3c00032
Zachary R. Sitte, Abel Andre Miranda Buzetta, Sarina J. Jones, Zhi-Wei Lin, Nathan Ashbrook Whitman and Matthew R. Lockett*, 

Cell-based assays enable molecular-level studies of cellular responses to drug candidates or potential toxins. Transactivation assays quantify the activation or inhibition of nuclear receptors, key transcriptional regulators of gene targets in mamalian cells. One such assay couples the expression of luciferase to the transcriptional activity of estrogen receptor-alpha (ERα). While this assay is regularly used to screen for agonists and antagonists of the estrogen signaling pathway, the setup relies on monolayer cultures in which cells are plated directly onto the surface of cell-compatible plasticware. The tumor microenvironment is more than a collection of cancerous cells and is profoundly influenced by tissue architecture, the presence of extracellular matrices, and intercellular signaling molecules produced by non-cancerous neighboring cells (e.g., fibroblasts). There exists a need for three-dimensional culture platforms that can be rapidly prototyped to assess new configurations and readily produced in the large numbers needed for translational studies and screening applications. Here, we demonstrate the utility of the paper-based culture platform to probe the effects of intercellular signaling between two cell types. We used paper scaffolds to generate tumor-like environments, forming a defined volume of breast cancer cells suspended in collagen. By placing the paper scaffolds in commercial 96-well plates, we compared monocultures of only breast cancer cells with coculture configurations containing fibroblasts in different locations that mimicked the stages of breast cancer progression. We show that ERα transactivation in the T47D-KBluc cell line is affected by the presence, number, and proximity of fibroblasts, and is a consequence of intercellular signaling molecules. After screening a small library of fibroblast-secreted signaling molecules, we showed that interleukin-6 (IL-6) was the primary driver of reduced estradiol sensitivity. These effects were mitigated in the coculture configurations by the addition of an IL-6 neutralizing antibody. We also assessed estrogen receptor expression and transcriptional regulation, further demonstrating the utility of the paper-based platform for detailed mechanistic studies.

以细胞为基础的化验可以在分子水平上研究细胞对候选药物或潜在毒素的反应。核受体是哺乳动物细胞中基因靶标的关键转录调控因子,转录激活测定可量化核受体的激活或抑制情况。其中一种检测方法将荧光素酶的表达与雌激素受体-α(ERα)的转录活性结合起来。虽然这种检测方法经常用于筛选雌激素信号通路的激动剂和拮抗剂,但其设置依赖于单层培养,在单层培养中,细胞被直接移植到与细胞兼容的塑料容器表面。 肿瘤微环境不仅仅是癌细胞的集合,还受到组织结构、细胞外基质的存在以及邻近非癌细胞(如成纤维细胞)产生的细胞间信号分子的深刻影响。目前需要一种三维培养平台,既能快速制作原型以评估新的配置,又能随时大量生产,满足转化研究和筛选应用的需要。 在这里,我们展示了纸基培养平台在探究两种细胞类型之间的细胞间信号转导效应方面的实用性。我们使用纸质支架生成类似肿瘤的环境,形成悬浮在胶原蛋白中的乳腺癌细胞的确定体积。通过将纸支架置于商用 96 孔板中,我们比较了仅有乳腺癌细胞的单培养与在不同位置含有成纤维细胞的共培养配置,后者模拟了乳腺癌的进展阶段。我们发现,T47D-KBluc 细胞系中的 ERα 转录活化受成纤维细胞的存在、数量和邻近程度的影响,是细胞间信号分子的结果。在筛选了一小部分成纤维细胞分泌的信号分子后,我们发现白细胞介素-6(IL-6)是降低雌二醇敏感性的主要驱动因素。在共培养配置中加入 IL-6 中和抗体可减轻这些影响。我们还评估了雌激素受体的表达和转录调控,进一步证明了纸质平台在详细机理研究中的实用性。
{"title":"Paper-Based Coculture Platform to Evaluate the Effects of Fibroblasts on Estrogen Signaling in ER+ Breast Cancers","authors":"Zachary R. Sitte,&nbsp;Abel Andre Miranda Buzetta,&nbsp;Sarina J. Jones,&nbsp;Zhi-Wei Lin,&nbsp;Nathan Ashbrook Whitman and Matthew R. Lockett*,&nbsp;","doi":"10.1021/acsmeasuresciau.3c00032","DOIUrl":"10.1021/acsmeasuresciau.3c00032","url":null,"abstract":"<p >Cell-based assays enable molecular-level studies of cellular responses to drug candidates or potential toxins. Transactivation assays quantify the activation or inhibition of nuclear receptors, key transcriptional regulators of gene targets in mamalian cells. One such assay couples the expression of luciferase to the transcriptional activity of estrogen receptor-alpha (ERα). While this assay is regularly used to screen for agonists and antagonists of the estrogen signaling pathway, the setup relies on monolayer cultures in which cells are plated directly onto the surface of cell-compatible plasticware. The tumor microenvironment is more than a collection of cancerous cells and is profoundly influenced by tissue architecture, the presence of extracellular matrices, and intercellular signaling molecules produced by non-cancerous neighboring cells (e.g., fibroblasts). There exists a need for three-dimensional culture platforms that can be rapidly prototyped to assess new configurations and readily produced in the large numbers needed for translational studies and screening applications. Here, we demonstrate the utility of the paper-based culture platform to probe the effects of intercellular signaling between two cell types. We used paper scaffolds to generate tumor-like environments, forming a defined volume of breast cancer cells suspended in collagen. By placing the paper scaffolds in commercial 96-well plates, we compared monocultures of only breast cancer cells with coculture configurations containing fibroblasts in different locations that mimicked the stages of breast cancer progression. We show that ERα transactivation in the T47D-KBluc cell line is affected by the presence, number, and proximity of fibroblasts, and is a consequence of intercellular signaling molecules. After screening a small library of fibroblast-secreted signaling molecules, we showed that interleukin-6 (IL-6) was the primary driver of reduced estradiol sensitivity. These effects were mitigated in the coculture configurations by the addition of an IL-6 neutralizing antibody. We also assessed estrogen receptor expression and transcriptional regulation, further demonstrating the utility of the paper-based platform for detailed mechanistic studies.</p>","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"3 6","pages":"479–487"},"PeriodicalIF":0.0,"publicationDate":"2023-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsmeasuresciau.3c00032","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135141994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Screening of Indoor Transformation Products of Organophosphates and Organophosphites with an in Silico Spectral Database 利用硅谱数据库筛选有机磷和有机膦的室内转化产物
Q1 CHEMISTRY, ANALYTICAL Pub Date : 2023-10-05 DOI: 10.1021/acsmeasuresciau.3c00039
Steven Kutarna, Wanzhen Chen, Ying Xiong, Runzeng Liu, Yufeng Gong and Hui Peng*, 

Numerous transformation products are formed indoors, but they are outside the scope of current chemical databases. In this study, an in silico spectral database was established to screen previously unknown indoor transformation products of organophosphorus compounds (OPCs). An R package was developed that incorporated four indoor reactions to predict the transformation products of 712 seed OPCs. By further predicting MS2 fragments, an in silico spectral database was established consisting of 3509 OPCs and 28,812 MS2 fragments. With this database, 40 OPCs were tentatively detected in 23 indoor dust samples. This is the greatest number of OPCs reported to date indoors, among which two novel phosphonates were validated using standards. Twenty-four of the detected OPCs were predicted transformation products in which oxidation from organophosphites plays a major role. To confirm this, the in silico spectral database was expanded to include organophosphites for suspect screening in five types of preproduction plastics. A broad spectrum of 14 organophosphites was detected, with a particularly high abundance in polyvinyl chloride plastics and indoor end-user goods. This demonstrated the significant contribution of organophosphites to indoor organophosphates via oxidation, highlighting the strength of in silico spectral databases for the screening of unknown indoor transformation products.

在室内会形成许多转化产物,但这些产物不属于现有化学数据库的范围。本研究建立了一个硅学光谱数据库,用于筛选以前未知的有机磷化合物(OPCs)室内转化产物。开发的 R 软件包结合了四种室内反应,预测了 712 种 OPC 的转化产物。通过进一步预测 MS2 片段,建立了一个由 3509 种 OPC 和 28,812 个 MS2 片段组成的硅谱数据库。利用该数据库,在 23 份室内灰尘样本中初步检测出 40 种 OPC。这是迄今为止在室内报告的最多的 OPCs,其中两种新型膦酸盐已通过标准验证。在检测到的 OPC 中,有 24 种是预测的转化产物,其中有机膦酸盐的氧化作用发挥了主要作用。为了证实这一点,我们对硅学光谱数据库进行了扩展,将有机膦酸盐纳入其中,以便对五种生产前塑料中的可疑物质进行筛选。结果检测到 14 种有机膦酸盐,其中聚氯乙烯塑料和室内最终用户产品中的有机膦酸盐含量尤其高。这表明有机膦酸盐通过氧化作用对室内有机膦酸盐的贡献很大,突出了硅谱数据库在筛选未知室内转化产品方面的优势。
{"title":"Screening of Indoor Transformation Products of Organophosphates and Organophosphites with an in Silico Spectral Database","authors":"Steven Kutarna,&nbsp;Wanzhen Chen,&nbsp;Ying Xiong,&nbsp;Runzeng Liu,&nbsp;Yufeng Gong and Hui Peng*,&nbsp;","doi":"10.1021/acsmeasuresciau.3c00039","DOIUrl":"10.1021/acsmeasuresciau.3c00039","url":null,"abstract":"<p >Numerous transformation products are formed indoors, but they are outside the scope of current chemical databases. In this study, an in silico spectral database was established to screen previously unknown indoor transformation products of organophosphorus compounds (OPCs). An R package was developed that incorporated four indoor reactions to predict the transformation products of 712 seed OPCs. By further predicting MS<sup>2</sup> fragments, an in silico spectral database was established consisting of 3509 OPCs and 28,812 MS<sup>2</sup> fragments. With this database, 40 OPCs were tentatively detected in 23 indoor dust samples. This is the greatest number of OPCs reported to date indoors, among which two novel phosphonates were validated using standards. Twenty-four of the detected OPCs were predicted transformation products in which oxidation from organophosphites plays a major role. To confirm this, the in silico spectral database was expanded to include organophosphites for suspect screening in five types of preproduction plastics. A broad spectrum of 14 organophosphites was detected, with a particularly high abundance in polyvinyl chloride plastics and indoor end-user goods. This demonstrated the significant contribution of organophosphites to indoor organophosphates via oxidation, highlighting the strength of in silico spectral databases for the screening of unknown indoor transformation products.</p>","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"3 6","pages":"469–478"},"PeriodicalIF":0.0,"publicationDate":"2023-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsmeasuresciau.3c00039","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135482018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
ACS Measurement Science Au
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1