Dental Materials最新文献_第4页

Bioactive S-PRG materials in dental applications: A clinical evidence–based scoping review 生物活性S-PRG材料在牙科中的应用：临床循证范围综述。

IF 6.3 1区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Dental Materials

Pub Date : 2025-11-26 DOI: 10.1016/j.dental.2025.11.012

Nathan Felipini Ferreira , Renata de Oliveira Alves , Priscila Toninatto Alves de Toledo , Georgia Rondó Peres , Marcelle Danelon , Aimée Maria Guiotti , Gabriel Pereira Nunes

This scoping review (SR) aimed to map the clinical evidence on the effects of surface pre-reacted glass ionomer (S-PRG)-based materials on dental tissues and performance in restorative, preventive, and therapeutic applications. The SR followed the PRISMA-ScR statement, conducting a comprehensive search across multiple databases for articles published up to January 2025. Out of the 3914 records screened, 49 studies were included in the review. Clinical trials have demonstrated promising outcomes in various dental applications. In caries prevention and white spot lesion management, S-PRG-based sealants and varnishes showed bioactive effects, contributing to remineralization despite limitations in retention. In restorative treatments, S-PRG-based materials showed high clinical success rates, adequate marginal integrity, and minimal postoperative sensitivity, with performance similar to materials without S-PRG fillers. Favorable results were also observed in the treatment of non-carious cervical lesions, dentin hypersensitivity, and molar-incisor hypomineralization, reinforcing the versatility and clinical potential of S-PRG-containing materials. Based on findings, materials containing S-PRG fillers have demonstrated satisfactory clinical performance across various dental applications, especially as restorative agents, sealants, and desensitizers. However, further longer-term clinical studies are needed to confirm their durability, effectiveness over time, and optimize clinical application.

本文综述了基于表面预反应玻璃离聚体（S-PRG）的材料对口腔组织的影响及其在修复、预防和治疗方面的应用。根据PRISMA-ScR声明，SR在多个数据库中对截至2025年1月发表的文章进行了全面搜索。在筛选的3914项记录中，49项研究纳入了本综述。临床试验在各种牙科应用中显示出良好的结果。在预防龋齿和管理白斑方面，s - prg基密封剂和清漆显示出生物活性作用，有助于再矿化，尽管保留有限。在修复治疗中，基于S-PRG的材料表现出较高的临床成功率、足够的边缘完整性和最小的术后敏感性，其性能与没有S-PRG填充的材料相似。在治疗非龋齿性宫颈病变、牙本质过敏和磨牙-门牙低矿化方面也观察到良好的结果，加强了含s - prg材料的多功能性和临床潜力。基于研究结果，含有S-PRG填料的材料在各种牙科应用中表现出令人满意的临床性能，特别是作为修复剂、密封剂和脱敏剂。然而，需要进一步的长期临床研究来证实它们的耐久性，随时间的有效性，并优化临床应用。

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引用次数: 0

Dendrimer-based extrafibrillar demineralization for optimizing resin-dentin bond stability 基于树突的纤维外脱矿优化树脂-牙本质键稳定性。

IF 6.3 1区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Dental Materials

Pub Date : 2025-11-21 DOI: 10.1016/j.dental.2025.11.009

Yanning He , Yiwen Wang , Junhan Yang , Jingsi Chen , Jingwen Zhang , Yingying Yu , Yaping Gou , Franklin R. Tay

<div><h3>Objective</h3><div>A selectively extrafibrillar dentin demineralization concept has recently been developed that preserves intrafibrillar minerals within collagen fibrils to prevent exposure and activation of endogenous dentin proteases that are important contributor to collagen degradation within hybrid layers. The objective of the present study was to investigate the potential of using PEGylated PAMAM dendrimer (PEG-P) with high MWs (﹥40 kDa) as an antimicrobial extrafibrillar dentin demineralization conditioner to improve durability of resin-dentin bonds.</div></div><div><h3>Methods</h3><div>The chelation capacities of different concentrations of PEGylated PAMAM on calcium ion from dentin were measured using ICP-AES. The optimal degree and concentration of PEGylation for dentin bonding were determined by microtensile bond strength testing to make sure that bond strengths with the wet-bonding and dry-bonding technique before and after thermocycling was comparable to those of H<sub>3</sub>PO<sub>4</sub> etching. Morphological observation of PEG-P-based extrafibrillar demineralization were examined by FE-SEM and AFM. In-situ zymography was performed to directly evaluate gelatinolytic activity within hybrid layers. For antibacterial testing, dead/live bacterial staining was performed to evaluate 20 % PEG-P potent antibacterial effects on oral bacteria.</div></div><div><h3>Results</h3><div>The result of ICP-AES indicated that PEG-P had chelation capacities for Ca<sup>2 +</sup> . Wet- and dry-bonding with the 20 % PEG-P-based demineralization produced tensile bond strengths equivalent to H<sub>3</sub>PO<sub>4</sub>-based wet-bonding technique, and those bond strengths were maintained after thermocycling. Morphological observation of PEG-P-based extrafibrillar demineralization by FE-SEM showed partially demineralized dentin with mineralized collagen fibrils separated by detectable extrafibrillar spaces in both wet- and dry- bonding modes, whereas the H<sub>3</sub>PO<sub>4</sub>-ethched dentin produced completely demineralized collagen fibrils that collapsed and formed a smooth interface without porous spaces in the air-dried modes. Representative AFM images confirmed that the surface of dentin conditioned with 20 % PEG-P was significantly rougher than that of 37 % H<sub>3</sub>PO<sub>4</sub> group. In-situ zymography of gelatinolytic activity within hybrid layers created with PEG-P conditioning showed negligible fluorescence before and after thermocycling, as compared with the H<sub>3</sub>PO<sub>4</sub> group for both wet- and dry-bonding. Antibacterial testing showed that 20 % PEG-P possesses potent antibacterial effects on oral bacteria.</div></div><div><h3>Significance</h3><div>The PEG-P-based selectively extrafibrillar dentin demineralization preserves intrafibrillar minerals, prevents exposure and activation of endogenous dentin proteases, reduces collagen degradation by endogenous protease, and inhibits bacteria retained on dentin, wh

目的：最近提出了一种选择性的牙本质纤维外脱矿概念，保留胶原原纤维内的纤维内矿物质，以防止内源性牙本质蛋白酶的暴露和激活，而内源性牙本质蛋白酶是杂交层中胶原降解的重要因素。本研究的目的是研究高分子量（bbb40 kDa）聚乙二醇化PAMAM树状大分子（PEG-P）作为抗菌纤维外牙本质脱矿调节剂的潜力，以提高树脂-牙本质键的耐久性。方法：采用ICP-AES法测定不同浓度聚乙二醇化PAMAM对牙本质钙离子的螯合能力。通过微拉伸键合强度测试确定牙本质键合的最佳PEGylation程度和浓度，确保热循环前后湿键和干键技术的键合强度与H3PO4蚀刻的键合强度相当。采用FE-SEM和AFM对peg - p基纤维外脱矿进行形态学观察。原位酶谱法直接评价杂交层内明胶水解活性。抗菌试验采用活菌/死菌染色，评价20 % PEG-P对口腔细菌的有效抗菌作用。结果：ICP-AES结果表明PEG-P对Ca2 +具有螯合能力。采用20% % peg - p基脱矿剂进行湿键和干键的拉伸键强度与h3po4基湿键技术相当，并且在热循环后仍能保持这种强度。通过FE-SEM对peg - p基牙本质脱矿的形态学观察发现，在干湿结合模式下，部分脱矿的牙本质与矿化的胶原原纤维之间存在可检测到的纤维外间隙，而在风干模式下，H3PO4-ethched牙本质产生完全脱矿的胶原原纤维，胶原原纤维塌陷并形成光滑的界面，没有多孔空间。具有代表性的AFM图像证实，20 % PEG-P处理的牙本质表面明显粗糙于37 % H3PO4组。原位酶谱分析显示，与H3PO4组相比，用PEG-P调节形成的杂化层中明胶水解活性在热循环前后的荧光可以忽略不计，无论是湿键还是干键。抗菌试验表明，20% % PEG-P对口腔细菌具有较强的抗菌作用。意义：peg - p基牙本质选择性脱矿保存了牙本质内的矿物质，防止了内源性牙本质蛋白酶的暴露和活化，减少了内源性蛋白酶对胶原的降解，抑制了保留在牙本质上的细菌，是提高树脂-牙本质结合耐久性的重要因素。

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引用次数: 0

Comparative cytotoxicity of 45S5 bioactive glass and strontium-calcium silicate glass on human gingival fibroblasts over 72 h 45S5生物活性玻璃和硅酸锶钙玻璃对人牙龈成纤维细胞72 h的细胞毒性比较。

IF 6.3 1区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Dental Materials

Pub Date : 2025-11-21 DOI: 10.1016/j.dental.2025.11.014

Fatima Adel Al Mansour , Rasha A. Alamoush , Julian D. Satterthwaite , Xiaohui Chen , Nikolaos Silikas

Aims

This study aimed to evaluate the ion release behaviour and the pH change of melt-derived bioactive glass (45S5) conditioned media compared with mixed strontium calcium silicate glass (Sr40) and to evaluate the influence of BG45S5 and Sr40 conditioned media on the cytotoxicity of gingival fibroblast (GF) cells.

Methods

Melt-derived bioactive glass (BG45S5) and a mixed strontium calcium silicate glass (Sr40) were prepared and supplied by Cera Dynamic Ltd. The pH and ion release profile from each glass were evaluated by immersing glass particles (< 38 µm) in gingival fibroblast (GF) media (50 ml) for up to 72 h at room temperature. GF cells were seeded into 24-well plates and exposed to BG45S5 and Sr40 glass-conditioned media at 1, 3, and 5 days in vitro to evaluate their cytotoxicity on GF. The cytotoxic effects were assessed via a lactate dehydrogenase (LDH) assay and then measured via absorbance using a Biochrom Asys UVM 340 Microplate (Biochrom, Cambridge, UK) plate reader.

Results

The pH increase and ion release from 45S5 glass were greater than Sr40 glass. 45S5 showed a drastic increase in pH from 7.5 to 8.7 at 24 h, followed by a constant pH for up to 3 days (72 h). A less pronounced pH increase was evident for Sr40, with the pH increasing from 7.5 to 7.6 at 24 h, followed by a gradual increase to 7.8 for up to 3 days. Concerning cytotoxicity, GF exposed to 45S5 media presented a significant increase in LDH release at 1 and 3 days in vitro compared to no glass media. However, for the Sr40 glass, no significant increase in cytotoxicity was observed at 1 and 3 days. There was a significant increase in LDH detected in the media compared with the low control (no glass media) at day 5.

Significance

This study emphasises the superior biocompatibility of Sr40 glass compared to 45S5 for gingival fibroblasts. Sr40’s lower cytotoxicity indicates its potential as a safer alternative for coating dental implants.

目的：评价熔融生物活性玻璃（45S5）条件培养基与混合锶钙硅酸玻璃（Sr40）条件培养基的离子释放行为和pH变化，并评价BG45S5和Sr40条件培养基对牙龈成纤维细胞（GF）细胞毒性的影响。方法：制备熔融衍生生物活性玻璃（BG45S5）和混合锶钙硅酸玻璃（Sr40），并由Cera Dynamic有限公司提供。在室温下，将玻璃颗粒（< 38 µm）浸泡在牙龈成纤维细胞（GF）培养基（50 ml）中长达72 小时，以评估每个玻璃的pH和离子释放情况。将GF细胞接种于24孔板中，分别于体外1、3和5天暴露于BG45S5和Sr40玻璃培养基中，以评估其对GF的细胞毒性。通过乳酸脱氢酶（LDH）测定评估细胞毒性作用，然后使用Biochrom Asys UVM 340微孔板（Biochrom, Cambridge， UK）读板仪进行吸光度测定。结果：45S5玻璃的pH升高和离子释放量均大于Sr40玻璃。45S5在24 h时pH值从7.5急剧增加到8.7，随后pH值保持不变长达3天（72 h）。Sr40的pH值增加不明显，在24 h时pH值从7.5增加到7.6，然后逐渐增加到7.8，持续3天。在细胞毒性方面，GF暴露于45S5培养基中，在体外1天和3天的LDH释放量明显增加，而没有玻璃培养基。然而，对于Sr40玻璃，在第1天和第3天没有观察到明显的细胞毒性增加。与低对照（无玻璃培养基）相比，培养液中LDH的检测在第5天显著增加。意义：本研究强调了与45S5相比，Sr40玻璃对牙龈成纤维细胞具有更好的生物相容性。Sr40较低的细胞毒性表明其作为牙种植体涂层更安全的替代品的潜力。

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引用次数: 0

Biomechanical properties of 3D-printed resin composites compared with subtractive CAD/CAM materials and direct light-cured composites: Color stability 3d打印树脂复合材料与减法CAD/CAM材料和直接光固化复合材料的生物力学性能比较：颜色稳定性。

IF 6.3 1区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Dental Materials

Pub Date : 2025-11-20 DOI: 10.1016/j.dental.2025.11.010

René Daher , Fah Prathanporn Yantarasri , Ivo Krejci , Julian Grégoire Leprince , Stefano Ardu , Jae-Hyun Lee

Objectives

This study aimed to evaluate the color stability of four three-dimensionally-printed (3D) resin composites, and compare them with nine other commonly used subtractive computer-aided designing/computer-aided manufacturing (CAD/CAM) materials and resin composites for direct restoration.

Methods

Totally, 130 specimens (n = 10) were prepared from four different 3D-printed resin composites (Brilliant Print Experimental, Crowntec, Temp PRINT, VarseoSmile Crown Plus), six subtractive CAD/CAM materials (Initial Lisi Block, IPS e.max CAD, Katana Avencia, Telio CAD, Tetric CAD, Vita Enamic) and three direct light-curing resin composites (Clearfil Majesty ES-2 Classic, Tetric EvoCeram, and G-ænial Universal Injectable), and immersed in five solutions (water, tea, red wine, coffee and curry) for 28 days. Initial and final color values (CIE L* a* b*) were measured using a spectrophotometer over white and black backgrounds. Color changes were calculated (ΔE00) and statistically compared using two-way repeated measures analysis of variance and Fisher’s post-hoc test (α = 0.05).

Results

Statistical analysis of ∆E00 values revealed that two 3D-printed resin composites exhibited comparable discoloration to two subtractive CAD/CAM resin composites and one of the lithium disilicate over a black background. They revealed less discoloration compared with all direct resin composites (p < 0.05).

Significance

The investigated commercially-available 3D-printed resin composites are reliable to use in visible restorations, where common subtractive CAD/CAM and direct resin composites are indicated.

目的：评价四种三维打印树脂复合材料的颜色稳定性，并与其他九种常用的减色法计算机辅助设计/计算机辅助制造（CAD/CAM）材料和树脂复合材料进行直接修复比较。方法:用四种不同的3d打印树脂复合材料（Brilliant Print Experimental、Crowntec、Temp Print、VarseoSmile Crown Plus）、六种减法CAD/CAM材料（Initial Lisi Block、IPS e.max CAD、Katana Avencia、Telio CAD、Tetric CAD、Vita Enamic）和三种直接光固化树脂复合材料（Clearfil Majesty ES-2 Classic、Tetric EvoCeram和G- ekarenial Universal Injectable）共制备了130个样品（n = 10），并浸泡在五种溶液中(水、茶、葡萄酒、咖啡和咖喱)28天。在白色和黑色背景上使用分光光度计测量初始和最终颜色值（CIE L* a* b*）。计算颜色变化（ΔE00），并采用双向重复测量方差分析和Fisher事后检验进行统计学比较（α = 0.05）。结果：对∆E00值的统计分析显示，两种3d打印树脂复合材料在黑色背景上表现出与两种减法CAD/CAM树脂复合材料和一种二硅酸锂相当的变色。与所有直接树脂复合材料相比，它们显示出更少的变色（p ）意义：所研究的市售3d打印树脂复合材料可可靠地用于可见修复，其中指出了常见的减法CAD/CAM和直接树脂复合材料。

{"title":"Biomechanical properties of 3D-printed resin composites compared with subtractive CAD/CAM materials and direct light-cured composites: Color stability","authors":"René Daher , Fah Prathanporn Yantarasri , Ivo Krejci , Julian Grégoire Leprince , Stefano Ardu , Jae-Hyun Lee","doi":"10.1016/j.dental.2025.11.010","DOIUrl":"10.1016/j.dental.2025.11.010","url":null,"abstract":"<div><h3>Objectives</h3><div>This study aimed to evaluate the color stability of four three-dimensionally-printed (3D) resin composites, and compare them with nine other commonly used subtractive computer-aided designing/computer-aided manufacturing (CAD/CAM) materials and resin composites for direct restoration.</div></div><div><h3>Methods</h3><div>Totally, 130 specimens (n = 10) were prepared from four different 3D-printed resin composites (Brilliant Print Experimental, Crowntec, Temp PRINT, VarseoSmile Crown Plus), six subtractive CAD/CAM materials (Initial Lisi Block, IPS e.max CAD, Katana Avencia, Telio CAD, Tetric CAD, Vita Enamic) and three direct light-curing resin composites (Clearfil Majesty ES-2 Classic, Tetric EvoCeram, and G-ænial Universal Injectable), and immersed in five solutions (water, tea, red wine, coffee and curry) for 28 days. Initial and final color values (CIE L* a* b*) were measured using a spectrophotometer over white and black backgrounds. Color changes were calculated (ΔE00) and statistically compared using two-way repeated measures analysis of variance and Fisher’s post-hoc test (α = 0.05).</div></div><div><h3>Results</h3><div>Statistical analysis of ∆E00 values revealed that two 3D-printed resin composites exhibited comparable discoloration to two subtractive CAD/CAM resin composites and one of the lithium disilicate over a black background. They revealed less discoloration compared with all direct resin composites (p < 0.05).</div></div><div><h3>Significance</h3><div>The investigated commercially-available 3D-printed resin composites are reliable to use in visible restorations, where common subtractive CAD/CAM and direct resin composites are indicated.</div></div>","PeriodicalId":298,"journal":{"name":"Dental Materials","volume":"42 3","pages":"Pages 485-492"},"PeriodicalIF":6.3,"publicationDate":"2025-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145572810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of resin-modified glass ionomer functionalized with polyvalent metals and cyclic phosphates: Evaluation of mechanical properties, fluoride release, antibiofilm activity and cytotoxicity 多价金属和环磷酸盐功能化树脂改性玻璃离聚体的研制：力学性能、氟释放、抗生物膜活性和细胞毒性的评价。

IF 6.3 1区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Dental Materials

Pub Date : 2025-11-19 DOI: 10.1016/j.dental.2025.11.003

Marcelle Danelon , Christian Hannig , Sabine Basche , Diana Jünger , Heike Meißner , Celine Guder , Jörn Jaschinski , Torsten Sterzenbach

Objective

This in vitro study evaluated the effect of incorporating stannous chloride (SnCl₂) and sodium trimetaphosphate (TMP) into resin modified glass ionomer cement (RMGIC) on its mechanical properties, fluoride release, antimicrobial/antibiofilm activity, and cytotoxicity.

Methods

Six experimental cements were tested: (1) RMGIC; (2) RMGIC+14 %TMP; (3) RMGIC+400 ppm SnCl₂; (4) RMGIC+ 14 %TMP+400 ppm SnCl₂; (5) RMGIC+800 ppm SnCl₂; (6) RMGIC+14 %TMP+800 ppm SnCl₂. Degree of conversion (%DC), diametral/compressive/flexural tensile strength (DTS, CS and FS), Vickers hardness surface (VHS) and surface roughness (Ra) were determined. Fluoride release (F) was assessed over 15 days in demineralizing/remineralizing solutions. Antimicrobial/antibiofilm effects were analyzed using agar diffusion test and DAPI Staining against Streptococcus mutans (S. mutans). Cytotoxicity was evaluated on human primary gingival fibroblasts/epithelial cells.

Results

The incorporation of TMP and SnCl₂ enhanced the mechanical performance of the RMGIC. The group containing TMP with 400 ppm SnCl₂ showed the highest DTS and CS (p < 0.05). Ra and VHS varied slightly among groups but remained within acceptable ranges. F release peaked within the first 24 h, particularly in formulations containing TMP with SnCl₂ (p < 0.05), and declined gradually thereafter. SnCl₂, alone or combined with TMP, significantly reduced S. mutans viability (p < 0.05), as confirmed by bacterial counts and fluorescence staining. Cytotoxicity remained low in all groups containing SnCl₂.

Significance

The modification of RMGIC with SnCl₂ and/or TMP showed potential to enhance certain functional properties. Biocompatibility concerns with TMP and the short evaluation period limit definitive conclusions. Further long-term studies are needed to confirm safety and clinical efficacy, particularly regarding fluoride release and antimicrobial effects.

目的：体外研究氯化亚锡（SnCl 2）和三偏磷酸钠（TMP）对树脂改性玻璃离子水泥（RMGIC）力学性能、氟释放、抗菌/抗生物膜活性和细胞毒性的影响。方法：对6种实验水泥进行检测：(1)RMGIC；(2) RMGIC + 14 % TMP;(3) RMGIC+400 ppm SnCl₂；(4) RMGIC+ 14 %TMP+400 ppm SnCl₂；(5) RMGIC+800 ppm SnCl₂；(6) RMGIC+14 %TMP+800 ppm SnCl₂。测定了转换度（%DC）、直径/压缩/弯曲抗拉强度（DTS、CS和FS）、表面维氏硬度（VHS）和表面粗糙度（Ra）。在脱矿/再矿化溶液中评估氟化物释放(F)超过15天。采用琼脂扩散试验和DAPI染色分析抗变形链球菌（S. mutans）的抗菌/抗菌膜效果。对人原代牙龈成纤维细胞/上皮细胞进行了细胞毒性评价。结果：TMP和SnCl 2的掺入增强了RMGIC的力学性能。含有400 ppm SnCl 2的TMP组显示出最高的DTS和CS （p ）。意义：用SnCl 2和/或TMP修饰RMGIC显示出增强某些功能特性的潜力。关于TMP的生物相容性问题和较短的评估期限制了明确的结论。需要进一步的长期研究来确认安全性和临床疗效，特别是关于氟化物释放和抗菌效果。

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引用次数: 0

Modification of a universal adhesive with CHX- or DOX-loaded TiO2 nanotubes as an anti-MMP strategy 负载CHX或dox的二氧化钛纳米管作为抗mmp策略的改性通用粘合剂。

IF 6.3 1区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Dental Materials

Pub Date : 2025-11-18 DOI: 10.1016/j.dental.2025.11.006

Fernanda Sandes de Lucena , Érika Soares Bronze-Uhle , Matthew Logan , Steven Lewis , Marjorie de Oliveira Gallinari , Paulo Noronha Lisboa Filho , Carla Castiglia Gonzaga , Diana Gabriela Soares dos Passos , Carmem Silvia Pfeifer , Adilson Yoshio Furuse

Objectives

This study evaluated whether titanium dioxide nanotubes (TiO₂-nts), loaded with chlorhexidine (CHX) or doxycycline (DOX) and incorporated into a universal adhesive, could sustain matrix metalloproteinase (MMP) inhibition and enhance long-term bond durability without compromising polymerization or cytocompatibility. The influence of 3-aminopropyltrimethoxysilane (APTMS) surface functionalization on drug release, adhesive conversion, and mechanical performance was also investigated.

Methods

TiO₂-nts were synthesized via hydrothermal processing and either left unmodified or functionalized with APTMS. CHX or DOX was loaded into both nanotube types and incorporated into a universal adhesive (5 wt%). Nanotube morphology and functionalization were assessed using TEM, SEM/EDS, XRD, and TGA. Drug loading and release were quantified by HPLC. Degree of conversion (DC) was measured via FTIR. Microtensile bond strength (μTBS) was evaluated after 24 h and 6 months in both etch-and-rinse and self-etch modes (n = 6). MMP activity was assessed using a colorimetric assay (n = 8), and transdentinal cytotoxicity was analyzed using MTT and Live/Dead assays with human dental pulp stem cells (n = 6). Data were analyzed using one-way ANOVA and Tukey’s post-hoc test (α=0.05).

Results

DOX exhibited higher loading efficiency and more sustained release than CHX in both nanotube types (p < 0.01). APTMS functionalization improved nanotube dispersion and preserved DC (76–82 %), while unmodified nanotubes significantly reduced DC (24–32 %, p < 0.001 vs. control). Despite lower DC, unmodified drug-loaded nanotubes maintained bond strength over 6 months with < 15 % μTBS reduction, whereas APTMS-functionalized groups exhibited greater degradation (30–40 % loss, p < 0.05). All drug-loaded adhesives inhibited MMP activity by > 60 % relative to control (p < 0.001). No significant cytotoxic effects were observed across any group (p > 0.05).

Significance

Incorporating TiO₂ nanotubes into adhesives highlighted a trade-off between polymerization and the duration of drug release. While drug release was short-lived, all modified adhesives reduced MMP activity and showed differences in bond stability depending on nanotube functionalization. These results provide insight into how nanotube surface chemistry influences adhesive performance and point to strategies for optimizing conversion and therapeutic longevity in future adhesive systems.

目的：本研究评估二氧化钛纳米管（tio_2 -nts），负载氯己定（CHX）或强力霉素（DOX）并结合到通用粘合剂中，是否可以维持基质金属蛋白酶（MMP）抑制并提高长期粘合耐久性，而不影响聚合或细胞相容性。研究了3-氨基丙基三甲氧基硅烷（APTMS）表面功能化对药物释放、粘附转化和力学性能的影响。方法：采用水热法合成tio2 -nts，经APTMS修饰或功能化。CHX或DOX被加载到两种纳米管中，并加入到通用粘合剂中（5 wt%）。采用TEM、SEM/EDS、XRD和TGA对纳米管的形貌和功能化进行了表征。用高效液相色谱法测定药量和释放度。通过FTIR测量转换度（DC）。在24 h和6个月后，在蚀刻-冲洗和自蚀刻模式下评估微拉伸粘结强度（μTBS）（n = 6）。使用比色法评估MMP活性（n = 8），使用人牙髓干细胞（n = 6）的MTT和Live/Dead试验分析牙髓细胞毒性。数据分析采用单因素方差分析和Tukey事后检验（α=0.05）。结果：DOX在两种纳米管中均比CHX表现出更高的负载效率和更强的缓释（p 60 %）（p 0.05）。意义：将二氧化钛纳米管加入粘合剂中，突出了聚合和药物释放时间之间的权衡。虽然药物释放是短暂的，但所有改性胶粘剂都降低了MMP活性，并且根据纳米管功能化表现出不同的键稳定性。这些结果为纳米管表面化学如何影响粘附性能提供了见解，并为优化未来粘附系统的转化和治疗寿命提供了策略。

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引用次数: 0

A novel statherin-derived phosphomimetic peptide (DDSt15) presents affinity for hydroxyapatite and effectively protects against intrinsic dental erosion 一种新的石蜡素衍生的拟磷肽（DDSt15）具有羟基磷灰石的亲和力，可以有效地保护牙齿免受内在侵蚀。

IF 6.3 1区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Dental Materials

Pub Date : 2025-11-14 DOI: 10.1016/j.dental.2025.11.007

Carolina Ruis Ferrari , Paulo Augusto Netz , Karolyne Sayuri de Araujo Kitamoto , Anna Clara de Mello Zonta , Hannah Zomignan Barros , Monique Malta Francese , Talita Mendes Oliveira Ventura , Éven Akemi Taira , Reinaldo Marchetto , Marília Afonso Rabelo Buzalaf

This study aimed to investigate the interaction mechanisms of the statherin derived phosphomimetic peptide (DDSt15) with hydroxyapatite through molecular dynamics simulations and Quartz Crystal Microbalance with Dissipation Monitoring (QCM-D) analysis and evaluate its protective effect against intrinsic enamel erosion in vitro. The peptide was engineered by mutating two residues in the statherin derived peptide (StatpSpS), and its topology was established using CHARMM-GUI for GROMACS simulations. A 500 ns molecular dynamics simulation assessed the peptide's behavior with hydroxyapatite. Additionally, DDSt15 was synthesized using a solid-phase method. Interactions of DDSt15 with saliva and hydroxyapatite surface were analyzed using QCM-D to evaluate the effect of acquired pellicle enrichment with this peptide. In vitro experiments were performed on bovine enamel samples treated with concentrations of DDSt15 at 0.94 × 10⁻⁵ M, 1.88 × 10⁻⁵ M, 3.76 × 10⁻⁵ M, and 1.88 × 10⁻⁵ M StatpSpS, with negative and positive control groups receiving deionized water and the commercial solution Elmex Erosion Protection®, respectively. Erosive challenges were applied using 0.01 M hydrochloric acid (pH 2.3) once/day for 3 consecutive days, and enamel hardness and reflection intensity were assessed pre- and post-treatment. Results indicated that DDSt15 exhibited affinity for the hydroxyapatite surface, and the pellicle formed with DDSt15 demonstrated significant properties, as observed in QCM-D analyses. In vitro experiments revealed that this peptide effectively enhanced enamel resistance to acid-induced erosion compared to control treatments. These results suggest the potential of DDSt15, particularly at the concentration of 0.94 × 10⁻⁵ M, to contribute to the development of strategies for acquired pellicle modulation aimed at preventing intrinsic enamel erosion.

本研究旨在通过分子动力学模拟和石英晶体微平衡耗散监测（QCM-D）分析，探讨石蜡素衍生的拟磷肽（DDSt15）与羟基磷灰石的相互作用机制，并评价其对体外内生釉质侵蚀的保护作用。通过突变startherin衍生肽（StatpSpS）中的两个残基来设计该肽，并使用CHARMM-GUI建立其拓扑结构，进行GROMACS模拟。500 ns分子动力学模拟评估了肽与羟基磷灰石的行为。此外，采用固相法合成了DDSt15。利用QCM-D分析了DDSt15与唾液和羟基磷灰石表面的相互作用，以评价该肽对获得性膜富集的影响。用DDSt15浓度分别为0.94 × 10 -⁻- M、1.88 × 10-⁻- M、3.76 × 10 -⁻- M和1.88 × 10-5 M的stpsps处理过的牛牙釉质样品进行体外实验，阴性对照组和阳性对照组分别接受去离子水和Elmex腐蚀保护®商业溶液。采用0.01 M盐酸（pH 2.3），每天1次，连续3 d，评价处理前后牙釉质硬度和反射强度。结果表明，DDSt15对羟基磷灰石表面具有亲和力，并且在QCM-D分析中发现，DDSt15形成的膜具有显著的特性。体外实验表明，与对照处理相比，该肽有效地增强了牙釉质对酸侵蚀的抵抗力。这些结果表明，DDSt15的潜力，特别是在浓度为0.94 × 10⁻- 5 M时，有助于制定旨在预防内在牙釉质侵蚀的获得性膜调节策略。

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引用次数: 0

What enhancement could β-titanium bring to oral implantology? β钛对口腔种植有哪些增强作用？

IF 6.3 1区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Dental Materials

Pub Date : 2025-11-14 DOI: 10.1016/j.dental.2025.11.005

Clarisse Montet , Hazem Abouelleil , Cerine Labadi , Rémy Gauthier , Arnaud Lafon , Nina Attik

Objectives

Titanium and its alloys are widely used in medicine, particularly in dentistry, due to their excellent biocompatibility and mechanical properties. However, limitations associated with commercially pure Titanium (cp-Ti) grade IV and conventional alloys such as Ti-6Al-4V require the development of alternative materials. This short review aims to highlight the potential of β-phase Titanium alloys as next-generation materials for oral implantology.

Methods

This review is based on ISO standards and clinical requirements related to dental biomaterials, with a particular focus regarding mechanical properties and biocompatibility. Literature research, was conducted targeting studies on both the mechanical and biological performance of Titanium-based materials, with emphasis on β-phase Titanium alloys.

Results

Cp-Ti grade IV remains the most commonly used Titanium for dental implants due to its long-standing clinical use, however, it exhibits some limitations, including a high elastic modulus and limited wear resistance. Ti-6Al-4V provides enhanced mechanical strength but raises biocompatibility concerns due to potential Aluminium and Vanadium ion release. β-phase Titanium alloys, with their body-centered cubic structure, demonstrate reduced elastic modulus closer to that of bone, enhanced mechanical strength and improved corrosion resistance, making them promising candidates for dental implants applications.

Conclusion

β-phase Titanium alloys present significant potential to overcome the limitations of current Titanium materials used in oral implantology. Their favorable biomechanical behavior and the absence of released toxic elements, suggest improved implant performance and longevity. Nonetheless, rigorous in vitro and in vivo investigations are essential to confirm their safety, biocompatibility, and long-term clinical outcome before widespread clinical adoption.

目的：钛及其合金因其优异的生物相容性和力学性能而广泛应用于医学，特别是牙科。然而，商业纯钛（cp-Ti） IV级和传统合金（如Ti-6Al-4V）的局限性要求开发替代材料。这篇简短的综述旨在强调β相钛合金作为下一代口腔种植材料的潜力。方法：本综述基于ISO标准和牙科生物材料的临床要求，特别关注力学性能和生物相容性。对钛基材料的力学性能和生物学性能进行了针对性的研究，重点研究了β相钛合金。结果：由于Cp-Ti IV级钛在临床的长期使用，它仍然是牙种植体中最常用的钛材料，然而，它表现出一些局限性，包括高弹性模量和有限的耐磨性。Ti-6Al-4V提供增强的机械强度，但由于潜在的铝和钒离子释放而引起生物相容性问题。β相钛合金具有体心立方结构，弹性模量更接近骨，机械强度更高，耐腐蚀性能更好，是牙种植体的理想材料。结论：β相钛合金具有很大的潜力，可以克服目前钛材料在口腔种植中的局限性。它们良好的生物力学性能和不释放有毒元素，表明种植体的性能和寿命得到改善。尽管如此，在广泛临床应用之前，严格的体外和体内研究对于确认其安全性、生物相容性和长期临床结果至关重要。

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引用次数: 0

Fabricated modified compomer bearing CF/SBA-15 nanomaterials: Physicochemical and antibacterial properties 含CF/SBA-15纳米材料的改性复合材料：物理化学和抗菌性能。

IF 6.3 1区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Dental Materials

Pub Date : 2025-11-14 DOI: 10.1016/j.dental.2025.11.008

Fatma Nur Kızılay , Mustafa Aydınbelge , Sezer Demirbuğa , Kevser Kolçakoğlu , Nilay Ildız , Serkan Dayan

Objectives

While compomers are widely used in pediatric dentistry, their antibacterial potential and fluoride release remain limited. This study aimed to evaluate the antibacterial and mechanical properties of compomers modified with different concentrations of calcium fructoborate–loaded mesoporous silica (CF@SBA-15) nanoparticles.

Methods

CF was synthesized via the Miljković method and loaded into SBA-15. The resulting CF@SBA-15 nanomaterial was incorporated into a compomer at 0.5 % (Group 1) and 1.0 % (Group 2) by weight. Surface roughness, microhardness, and degree of conversion (DC) were evaluated. Characterization was performed using FT-IR, SEM–EDX, and TGA analyses. Boron release was quantified at 1, 24, 72, and 96 h using ICP-MS. Antibacterial activity against Streptococcus mutans and Lactobacillus casei was assessed by the direct contact test (DCT). Statistical significance was set at p < 0.05.

Results

Surface roughness and microhardness values increased significantly with higher CF@SBA-15 concentrations (p < 0.001), with Group 2 exhibiting the highest mean values. DC was significantly higher in both experimental groups than in the control (p = 0.009). Boron release demonstrated a progressive, concentration-dependent pattern, with Group 2 showing greater cumulative release (p = 0.009). Both nanoparticle-modified groups exhibited significantly stronger antibacterial effects compared with the control (p < 0.01).

Conclusions

Incorporating CF@SBA-15 nanoparticles into compomers enhanced antibacterial efficacy while preserving the essential physicochemical integrity of the material.

Clinical significance

CF@SBA-15–modified compomers offer a promising strategy for developing durable, antibacterial, and caries-resistant restorative materials, particularly suitable for pediatric dental applications.

目的：虽然复合材料广泛用于儿科牙科，但其抗菌潜力和氟化物释放仍然有限。本研究旨在评价不同浓度载聚果糖硼酸钙的介孔二氧化硅（CF@SBA-15）纳米颗粒修饰的复合材料的抗菌性能和力学性能。方法：采用miljkovovic法合成CF，并将其装入SBA-15中。将得到的CF@SBA-15纳米材料以0.5 %（第一组）和1.0 %（第二组）的重量掺入复合材料中。评估了表面粗糙度、显微硬度和转换度（DC）。使用FT-IR， SEM-EDX和TGA分析进行表征。在1、24、72和96 h时用ICP-MS定量硼的释放。采用直接接触试验（DCT）评价其对变形链球菌和干酪乳杆菌的抑菌活性。结果：CF@SBA-15浓度越高，表面粗糙度和显微硬度值越高（p ）。结论：CF@SBA-15纳米颗粒在保持材料基本物理化学完整性的同时，增强了复合材料的抗菌效果。临床意义：CF@SBA-15-modified复合材料为开发耐用、抗菌和抗龋修复材料提供了一个有前途的策略，特别适合儿童牙科应用。

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引用次数: 0

Effect of cooling rate on the microstructural and mechanical properties of speed-sintered monolithic zirconia 冷却速率对快速烧结整体氧化锆组织和力学性能的影响。

IF 6.3 1区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Dental Materials

Pub Date : 2025-11-08 DOI: 10.1016/j.dental.2025.11.002

Stevan M. Čokić , Jef Vleugels , Bart Van Meerbeek , Fei Zhang

Objectives

To evaluate the effect of different speed-sintering cooling rates on the phase composition, microstructure, optical and mechanical properties of monolithic zirconia.

Methods

Speed-sintered ZirCAD LT (Ivoclar) (LT_1200; 5 min at 1480 °C; oven-door opening and air-cooling: 1200 °C) and Katana STML (Kuraray Noritake) (STML_800; 30 min at 1560 °C; oven-door opening and air-cooling: 800 °C) were compared to speed-sintered ZirCAD LT and Katana STML with a steady cooling rate and oven-door opening at 300 °C (LT_300 and STML_300, respectively). Density was measured by Archimedes’ principle; composition by XRF; phase by XRD; grain size by SEM; translucency parameter by spectrophotometry. Vickers hardness, indentation fracture toughness, and biaxial strength were also assessed.

Results

Regardless of the cooling rate, ZirCAD LT and Katana STML revealed similar density, chemical composition and grain size. Both 3Y-TZP ZirCAD LT_1200/300 zirconia had lower cubic ZrO₂-phase content, and lower Y₂O₃ content in the remaining tetragonal ZrO₂ phases compared to 5Y-PSZ Katana STML_800/300 zirconia. In contrast to 3Y-TZP, 5Y-PSZ contained a high amount of metastable t’-phase. TP of ZirCAD LT and Katana STML were not affected by the cooling rate. The hardness and fracture toughness of both 3Y-TZP and 5Y-PSZ were not influenced by cooling rate variations. Steady cooling significantly diminished flexural strength and mechanical reliability of Katana STML_300, while it did not have any effect on the ZirCAD LT zirconia grades.

Significance

Steady cooling during speed-sintering did not significantly influence the performance of 3Y-TZP but can negatively influence flexural strength and mechanical reliability of 5Y-PSZ zirconia ceramics.

目的：研究不同速度烧结冷却速率对整体氧化锆相组成、显微组织、光学性能和力学性能的影响。方法：将快速烧结的ZirCAD LT (Ivoclar) （LT_1200； 1480°C时5 min，炉门打开和风冷：1200°C）和Katana STML (Kuraray Noritake) （STML_800； 1560°C时30 min，炉门打开和风冷：800°C）与快速烧结的ZirCAD LT和Katana STML（分别为LT_300和STML_300）进行比较。用阿基米德原理测量密度；XRF分析；XRD相分析；SEM粒度；用分光光度法测定半透明参数。维氏硬度，压痕断裂韧性和双轴强度也进行了评估。结果：无论冷却速度如何，ZirCAD LT和Katana STML的密度、化学成分和晶粒尺寸相近。与5Y-PSZ Katana STML_800/300氧化锆相比，3Y-TZP ZirCAD LT_1200/300氧化锆的立方ZrO2相含量较低，其余四方ZrO2相中Y2O3含量较低。与3Y-TZP相比，5Y-PSZ含有大量的亚稳t′相。zrcad LT和Katana STML的TP不受冷却速率的影响。3Y-TZP和5Y-PSZ的硬度和断裂韧性不受冷却速率的影响。稳定冷却显著降低了Katana STML_300的弯曲强度和机械可靠性，而对ZirCAD LT氧化锆牌号没有任何影响。意义：快速烧结时的稳定冷却对3Y-TZP的性能影响不显著，但会对5Y-PSZ氧化锆陶瓷的抗弯强度和机械可靠性产生负面影响。

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引用次数: 0