Dental Materials最新文献

{"title":"Shrinkage stress is determined by the elastic modulus during shrinkage, not the final elastic modulus","authors":"Daranee Tantbirojn ,&nbsp;Jared Quave ,&nbsp;Anne E. Hill ,&nbsp;Clint S. Conner ,&nbsp;Antheunis Versluis","doi":"10.1016/j.dental.2025.07.022","DOIUrl":"10.1016/j.dental.2025.07.022","url":null,"abstract":"<div><h3>Objectives</h3><div>Polymerization shrinkage in restored teeth is implicated in restoration longevity. This study explored how development of shrinkage and elastic modulus of resin-based composites interact and discussed the level of residual stress they create.</div></div><div><h3>Methods</h3><div>Residual stress was assessed by cuspal flexure of typodont premolars with 3 × 3 mm mesio-occlusal-distal preparations. The teeth were restored with one of three bulk-fill flowable composites (everX Flow Short-fiber Reinforced, GC; Filtek Bulk Fill, 3 M; or SDR Flow+ Bulk Fill, Dentsply Sirona) placed in one bulk increment. Tooth surfaces were scanned before (‘baseline’) and after restoration (15 min and 24hrs). Cuspal flexure was determined by comparing scans of restored teeth with their baseline. Bond integrity was verified with dye. Post-gel shrinkage was measured using a strain gauge, and elastic modulus at 15 min and 24hrs using four-point bending. Sample size was 10. Results were analyzed with ANOVA statistics (α=0.05).</div></div><div><h3>Results</h3><div>Cuspal flexure differed significantly among composites (p = 0.001) but not between 15 min and 24hrs (p = 0.889). The fiber-reinforced composite caused significantly higher cuspal flexure compared to the conventional flowables (17<em>μ</em>m versus 7<em>μ</em>m) despite its lower post-gel shrinkage (0.53 versus 0.74 and 0.58 vol%). The elastic modulus of the fiber-reinforced composite was significantly higher at 15 min than the other composites (8 GPa versus 2 GPa), and increased significantly at 24hrs (12 GPa versus 8 GPa).</div></div><div><h3>Significance</h3><div>High elastic modulus development rate eliminated benefits associated with low post-gel shrinkage. Cuspal flexure and therefore residual shrinkage stress was largely determined by the early elastic modulus, when composite was still shrinking, not the mature (final) elastic modulus value.</div></div>","PeriodicalId":298,"journal":{"name":"Dental Materials","volume":"41 11","pages":"Pages 1388-1394"},"PeriodicalIF":6.3,"publicationDate":"2025-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144803082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Factors influencing the longevity of resin-based composite restorations","authors":"Akimasa Tsujimoto ,&nbsp;J.C. Thomas ,&nbsp;Daniel Caplan","doi":"10.1016/j.dental.2025.07.018","DOIUrl":"10.1016/j.dental.2025.07.018","url":null,"abstract":"<div><h3>Objectives</h3><div>The longevity of resin-based composite (RBC) restorations is influenced by many factors, and it is difficult to assess all of them in small data sets. This study uses a large data set from a university and its dental clinics to assess many potentially predictive factors of restoration survival.</div></div><div><h3>Methods</h3><div>This retrospective cohort study used de-identified data from electronic dental records of patients attending records the University of Iowa and its dental clinics. Multivariable Cox proportional hazards models were generated to determine which variables influenced the longevity of the restorations, and model selection via best subset selection on Bayesian Information Criterion (BIC) was used to select the best model.</div></div><div><h3>Results</h3><div>The number of surfaces covered by the restoration showed a very strong influence, with more surfaces leading to reduced longevity (HR 2.49 (CI 1.97–3.15), p &lt; 0.001 for 4 surfaces), but the inclusion of a proximal surface did not have a significant influence. The presence of an eating disorder (HR 1.72 (CI 1.15–2.56), p = 0.008), dry mouth (HR 1.20 (1.02–1.43), p = 0.033), or dental fear (HR 1.39 (CI 1.14–1.69), p = 0.001) also had a negative effect on restoration longevity, but bruxism/clenching did not. The type of filler used in the RBC, conventional or supra-nano spherical, also affected restoration longevity, with greater longevity for supra-nano spherical filler (HR 0.61 (CI 0.45–0.82), p = 0.001).</div></div><div><h3>Conclusions</h3><div>Type of provider showed no impact on restoration longevity in this study, and only a very limited number of patient factors were significant. Differences in the type of filler and the number of surfaces covered by the restoration did have a significant effect on restoration longevity.</div></div>","PeriodicalId":298,"journal":{"name":"Dental Materials","volume":"41 11","pages":"Pages 1395-1401"},"PeriodicalIF":6.3,"publicationDate":"2025-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144811491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Feasibility and safety of a novel laser-induced adhesive for orthodontic debonding","authors":"Yuyang Gan ,&nbsp;Yuan Yuan ,&nbsp;Yanning Gong ,&nbsp;Quan Fang ,&nbsp;Guangjun Liu ,&nbsp;Yuqian Li ,&nbsp;Denson Cao ,&nbsp;Daniel C.N. Chan ,&nbsp;Jianying Feng","doi":"10.1016/j.dental.2025.07.021","DOIUrl":"10.1016/j.dental.2025.07.021","url":null,"abstract":"<div><h3>Objectives</h3><div>This study evaluates the feasibility and safety of a laser-induced reversible adhesive (LIRA) by assessing intrapulpal temperature changes (ITC), shear bond strength (SBS) following laser irradiation, and optimal adhesive removal methods.</div></div><div><h3>Methods</h3><div>Thirty premolars were used to measure ITC under laser powers of 1 W, 2 W, and 3 W until the temperature exceeded the 5.6 ℃ threshold. For SBS testing, one hundred and twenty premolars were divided into twelve groups based on bracket type (metal/ceramic) and laser treatment (untreated, 1 W-4 s, 2 W-4 s, 1 W-8 s, 2 W-8 s, and 3 W-4 s). Adhesive remnant index (ARI) was recorded. Additionally, one hundred thirty-five premolars were assigned to nine groups based on laser power (1 W, 2 W, 3 W) and adhesive removal methods (ultrasonic scaler, tungsten carbide bur, diamond bur) for surface roughness and removal time assessment. Statistical analysis was performed using ANOVA, t-tests, and the Kruskal-Wallis test (α=0.05). Furthermore, the debonding resin-enamel interface and enamel surface morphology were observed by scanning electron microscopy (SEM).</div></div><div><h3>Results</h3><div>The time to exceed the temperature threshold decreased with increasing laser power. The 1 W-8 s and 2 W-8 s groups showed the lowest SBS (<em>p</em> &lt; 0.05). Ultrasonic scaler removal exhibited significantly lower enamel roughness (<em>p</em> &lt; 0.05), while removal time showed no significant difference at 2 W laser power (<em>p</em> &gt; 0.05).</div></div><div><h3>Significance</h3><div>LIRA may provide a potentially safer and more efficient strategy for orthodontic bracket bonding, achieving efficient debonding while minimizing enamel damage and ensuring pulp safety.</div></div>","PeriodicalId":298,"journal":{"name":"Dental Materials","volume":"41 11","pages":"Pages 1402-1410"},"PeriodicalIF":6.3,"publicationDate":"2025-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144803081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro Streptococcus mutans and Candida albicans adhesion on clear orthodontic aligners exposed to artificial saliva","authors":"Helena Reis de Souza Schacher ,&nbsp;Maiara Monteiro Oliveira ,&nbsp;Renata Medina-Silva ,&nbsp;Luciane Macedo de Menezes","doi":"10.1016/j.dental.2025.07.015","DOIUrl":"10.1016/j.dental.2025.07.015","url":null,"abstract":"<div><h3>Objectives</h3><div>Considering the pathogenic potential of some bacteria and yeast species in oral cavity this study aimed to analyze the capability of <em>Streptococcus mutans</em> and <em>Candida albicans</em> in artificial saliva (AS) to form biofilm on clear aligners.</div></div><div><h3>Methods</h3><div>Clear aligners fragments (Invisalign™) were subjected to a 7-day biofilm formation experiment in AS and tryptic soy broth (TSB) with <em>S. mutans</em> or <em>C. albicans</em> pre-inoculated. TSB and AS cultures were evaluated for microbial growth and biofilm formation with crystal violet assay. Quantitative data were statistically compared using the Mann-Whitney test and the Pearson correlation. Also, fragments were qualitatively processed using scanning electron microscopy (SEM) images.</div></div><div><h3>Results</h3><div>TSB induced the growth of <em>S. mutans</em> more effectively when compared to AS. Both media induced significant growth of <em>C. albicans</em> compared to biofilm, with TSB being significantly higher than AS. However, for both microorganisms, biofilm formation in AS and TSB did not show differences, indicating they may not depend on nutrients to adhere on clear aligners. SEM images showed bacteria and yeast cells adhering to the appliance’s inner surfaces for both media.</div></div><div><h3>Significance</h3><div>Aligners cover tooth enamel and gingival tissues for a long time and can interfere with oral microbiota and human health. Recognizing the real capacity of bacteria and yeast species to adhere to the surface of new aligners is important for raising awareness among professionals and patients.</div></div>","PeriodicalId":298,"journal":{"name":"Dental Materials","volume":"41 10","pages":"Pages 1332-1338"},"PeriodicalIF":6.3,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144768178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cavitation erosion: An efficient method for promoting osteogenesis and immunomodulation of titanium surface","authors":"Muqi Jiang ,&nbsp;Yasheng Sun ,&nbsp;Zhixiang Nie ,&nbsp;William M. Palin ,&nbsp;Lihua Qian ,&nbsp;Jukka P. Matinlinna ,&nbsp;Li Hu ,&nbsp;Zhen Zhang","doi":"10.1016/j.dental.2025.07.017","DOIUrl":"10.1016/j.dental.2025.07.017","url":null,"abstract":"<div><h3>Objectives</h3><div>Surface treatments of titanium dental implants can improve osseointegration and reduce the incidence of peri-implantitis. This study provides a new approach for surface modification by utilizing the principle of cavitation(CAV), which can generate high temperature and pressure with micro-jets acting on the surface.</div></div><div><h3>Methods</h3><div>Titanium surfaces were treated by CAV, and compared with a control group (sandblasted, large grit, and acid-etched, SLA). The microscopic morphology and roughness of the titanium surfaces, as well as their effect on osteoblast and macrophage biocompatibility were measured.</div></div><div><h3>Results</h3><div>Characteristic CAV pits were formed on the surface following CAV treatment, and roughness was significantly increased compared to the SLA surface (p &lt; 0.05). The CAV treatment not only promoted adhesion, proliferation, and osteogenic differentiation of osteoblasts more effectively than SLA, but also facilitated the polarization of macrophages towards an anti-inflammatory phenotype.</div></div><div><h3>Conclusions</h3><div>An innovative titanium implant surface treatment that utilises the CAV effect has been developed and demonstrates superior biocompatibility and ability to promote osteogenesis and immunomodulation compared with conventional surface treatment methods.</div></div><div><h3>Significance</h3><div>The CAV titanium surface treatment offers distinct advantages over SLA surface treatment, including superior osteogenic and immunomodulatory potential, along with a simpler and more environmentally friendly manufacturing process. These benefits establish CAV as a promising new strategy for enhancing titanium implant surfaces.</div></div>","PeriodicalId":298,"journal":{"name":"Dental Materials","volume":"41 11","pages":"Pages 1350-1360"},"PeriodicalIF":6.3,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144787868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Engineering quantum carbon dots unveiling quantum wave entanglement wave function on enamel substrate: A relativistic in-vitro study” [Dent. Mater., vol. 41, Issue 5 (2025), Pages 523–535]","authors":"Umer Daood ,&nbsp;Fabian Davamani Amalraj ,&nbsp;Kanwardeep Kaur ,&nbsp;Ranjeet Ajit Bapat ,&nbsp;Liang Lin Seow","doi":"10.1016/j.dental.2025.07.019","DOIUrl":"10.1016/j.dental.2025.07.019","url":null,"abstract":"","PeriodicalId":298,"journal":{"name":"Dental Materials","volume":"41 11","pages":"Page 1489"},"PeriodicalIF":6.3,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144768177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced antibacterial activity of plant polyphenol-modified robust polycaprolactone/gelatin electrospun coating on titanium implant","authors":"Yan Wang ,&nbsp;Li Liu ,&nbsp;Liyuan Zhou ,&nbsp;Huan Cheng ,&nbsp;Yifan Li ,&nbsp;Anni Wu ,&nbsp;Senjeet Sreekissoon ,&nbsp;Xiao Chen ,&nbsp;Peng Gao ,&nbsp;Lei Lu ,&nbsp;Zhennan Deng ,&nbsp;Jinsong Liu","doi":"10.1016/j.dental.2025.07.020","DOIUrl":"10.1016/j.dental.2025.07.020","url":null,"abstract":"<div><div>Postoperative infection significantly increases the risk of failure in titanium (Ti) dental implants. While the local application of antimicrobial agents can effectively mitigate these infections. Electrospinning has gained considerable attention for its ability to produce high-performance nanofibers suitable for localized drug release. Nevertheless, challenges such as weak interfacial bond with Ti surface limits its application as a surface coating strategy. In this study, we developed a novel electrospun coating composed of a plant polyphenol, epigallocatechin gallate (EGCG)-modified polycaprolactone (PCL) and gelatin (Gel), which enhanced its adhesion to the Ti surface through phenol-amine chemistry. This innovative approach significantly enhanced the interfacial bond strength between the nanofibers and the Ti surface, thereby improving the wear resistance of the coating. Additionally, the incorporation of rifampicin (Rif), a broad-spectrum antibiotic, into the EGCG-modified electrospun coating (Ti/NFs-0.5 %EG@Rif) enabled sustained Rif release over 28 days, providing potential long-term antibacterial activity. Both <em>in vitro</em> and <em>in vivo</em> studies demonstrated that the Ti/NFs-0.5 %EG@Rif coating exhibited significantly enhanced antibacterial and anti-inflammatory efficacy, along with excellent biocompatibility. In conclusion, the EGCG-modified electrospun coating represented a promising and cost-effective strategy for enhancing the functionality of Ti implants, with the potential to improve clinical outcomes in dental applications.</div></div>","PeriodicalId":298,"journal":{"name":"Dental Materials","volume":"41 11","pages":"Pages 1361-1375"},"PeriodicalIF":6.3,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144787869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Two-body fatigue wear resistance of 3D-printed composites evaluated using OCT","authors":"Fernando Antonio Reis Laurino ,&nbsp;Paulo Francisco Cesar ,&nbsp;Marcelo Munhóes Romano ,&nbsp;André Vicente Ritter ,&nbsp;Alireza Sadr","doi":"10.1016/j.dental.2025.07.014","DOIUrl":"10.1016/j.dental.2025.07.014","url":null,"abstract":"<div><h3>Objectives</h3><div>This study assessed the wear resistance of 3D-printed CAD/CAM composites using optical coherence tomography (OCT). OCT is a non-destructive and clinically applicable imaging technique that enables real-time and precise measurement of wear volume and subsurface changes without radiation exposure. The 3D-printed composites C&amp;B MFH, VarseoSmile CrownPlus, and Nanolab 3D were compared to the CAD/CAM composite milling block, Lava Ultimate, to determine whether their wear resistance was comparable to or exhibited significantly more significant material loss than the CAD/CAM block under cyclic loading in a chewing simulator.</div></div><div><h3>Methods</h3><div>Specimens of the three 3D-printed CAD/CAM composites—C&amp;B MFH, VarseoSmile CrownPlus, and Nanolab 3D—were designed as disks, 3D-printed or milled, then cleaned and polished before undergoing cyclic loading in a chewing simulator for 600,000 cycles. OCT analysis was used to measure wear extent and subsurface crack score. Statistical analysis was performed using one-way ANOVA and Kruskal-Wallis tests with post-hoc Bonferroni comparisons.</div></div><div><h3>Results</h3><div>Significant differences in wear resistance and crack development were observed among the composites. Lava Ultimate and C&amp;B MFH exhibited the highest wear resistance (0.7 ± 0.4 mm³ and 0.7 ± 0.2 mm³), while Nanolab 3D had the lowest and significantly different wear resistance (1.7 ± 0.8 mm³, p = 0.004). VarseoSmile CrownPlus showed intermediate volume loss (1.0 ± 0.7 mm³) with no significant difference from Nanolab 3D (p = 0.2). For vertical wear extent, Lava Ultimate (215.6 ± 40 μm) and C&amp;B MFH (310 ± 107 μm) had significantly lower wear than Nanolab 3D (546 ± 143 μm, p &lt; 0.001), while VarseoSmile CrownPlus (396 ± 131 μm) was not significantly different from Nanolab 3D (p = 0.8). A similar pattern was observed for cracks, with Lava Ultimate showing significantly different from the 3D-printed composites (p &lt; 0.001). Nanolab 3D exhibited the highest crack formation, showing a significant difference compared to all other materials (p &lt; 0.001), while no significant difference was found between C&amp;B MFH and VarseoSmile CrownPlus (p = 0.5).</div></div><div><h3>Significance</h3><div>In conclusion, the 3D-printed CAD/CAM composites demonstrated varying performance under cyclic loading, comparable or inferior to the CAD/CAM block, with OCT proving useful for evaluating composite wear resistance.</div></div>","PeriodicalId":298,"journal":{"name":"Dental Materials","volume":"41 11","pages":"Pages 1339-1349"},"PeriodicalIF":6.3,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144768183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of thickness and irradiance on the polymerization delay at the bottom surface of light-cured resin composites","authors":"Richard B. Price ,&nbsp;Alexandre P. Gareau ,&nbsp;Brett D. MacNeil ,&nbsp;Daniel Labrie ,&nbsp;Brian W. Darvell ,&nbsp;Frederick A. Rueggeberg ,&nbsp;Jeffrey W. Stansbury","doi":"10.1016/j.dental.2025.07.013","DOIUrl":"10.1016/j.dental.2025.07.013","url":null,"abstract":"<div><h3>Objective</h3><div>To examine the effects of the resin-based composite (RBC) thickness and incident irradiance on the time delay after the light turns on before polymerization starts at the bottom surface of the RBC.</div></div><div><h3>Methods</h3><div>The degree of conversion (DC) was determined using Fourier-transform infrared spectroscopy (FT-IR) at a rate of 13 measurements per second. Six commercial RBCs were tested at three thicknesses (0.2, 2 and 4 mm) and four incident irradiance values (472–3985 mW/cm²).</div></div><div><h3>Results</h3><div>There was a significant time delay before polymerization began at the bottom of each RBC. At 942 mW/cm², representative of many current LCUs, this delay ranged from 0.12 to 1.11 s at the bottom of 2 mm of RBC. When the thickness was increased to 4 mm, even with an irradiance of 1969 mW/cm², which represents high-power LCUs, the delay ranged from 0.12 to 5.17 s. The highest irradiance of 3985 mW/cm² did not fully overcome the inherent lag caused by the thickness of the RBC. At 3985 mW/cm², PowerFlow and PowerFill had the shortest delay times of 0.09 s and 0.30 s, respectively, at the bottom of a 4 mm thick RBC.</div></div><div><h3>Significance</h3><div>The presence of a time delay before photo-cured RBCs start to polymerize at the bottom surfaces must now be addressed clinically and when calculating reaction rates.</div></div>","PeriodicalId":298,"journal":{"name":"Dental Materials","volume":"41 10","pages":"Pages 1323-1331"},"PeriodicalIF":6.3,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144764251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assisted mineralization ability of proanthocyanidins in collagen scaffold and dentin","authors":"Fabiana Almeida Curylofo-Zotti ,&nbsp;Odair Bim-Júnior ,&nbsp;Ariene Leme-Kraus ,&nbsp;Silmara Aparecida Milori Corona ,&nbsp;Ana K. Bedran-Russo","doi":"10.1016/j.dental.2025.07.006","DOIUrl":"10.1016/j.dental.2025.07.006","url":null,"abstract":"<div><h3>Objectives</h3><div>To assess the potential mechanisms of an enriched <em>Vitis vinifera</em> (<em>Vve</em>) in mediating mineralization using both constituted type-1 collagen scaffolds and dentin matrix substrate.</div></div><div><h3>Methods</h3><div>An enriched formulation of oligomeric proanthocyanidins - <em>Vve</em> was compared to untreated substrates (C) and glutaraldehyde-treated substrate (GA). Biomineralization protocols included a simulated body fluid - SBF and a Ca-P supersaturated solutions for collagen scaffold and dentin matrix studies, respectively. Analytical methods included mineral content (FTIR), thermal behavior (DSC), crystal formation and Ca/P atomic ratio of crystal-like structure (SEM-EDS and TEM), and Ca²⁺ concentration (Ca-ISE). Data were statistically analyzed using one-way ANOVA and post-hoc tests (α=0.05).</div></div><div><h3>Results</h3><div>Untreated and <em>Vve</em> groups showed a higher amount of mineral normalized to the amount of collagen than the GA group (p &lt; 0.007 and p &lt; 0.019 respectively). Untreated and <em>Vve</em>-treated collagen scaffolds exhibited similar cumulative Ca²⁺ uptake (18.2 and 17.7 µg) and GA-treated scaffold had lower (15.8 µg). After 4 days in SBF solution, the highest endothermic peak was found at 81.4°C for <em>Vve</em>, followed by 78.7°C for GA and 57.6°C for untreated collagen. SEM-EDS analysis showed a well-organized layer of apatite crystals deposited around the collagen fibrils. <em>Vve</em> treatment promoted broad crystal nucleation over fibrils in a collagen scaffold. Unlike the GA-treated group, where fewer and sparsely distributed crystals were found, <em>Vve</em> group exhibited a thick and organized mineral layer over the dentin matrix.</div></div><div><h3>Significance</h3><div>Biomineralization occurs via a self-assembly process by which apatite crystals are deposited in an orderly fashion over the organic matrices of innate dentin. <em>Vve</em> may assist mineralization by preservation of the nucleation sites over the matrices modulating the rate of formation of apatite crystals.</div></div>","PeriodicalId":298,"journal":{"name":"Dental Materials","volume":"41 10","pages":"Pages 1313-1322"},"PeriodicalIF":6.3,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144717153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}