Dental Materials最新文献

{"title":"Bis-EMA/Bis-GMA ratio effects on resin-properties and impregnated fiber-bundles","authors":"Muhanad M. Hatamleh ,&nbsp;Adilson Yoshio Furuse","doi":"10.1016/j.dental.2024.07.001","DOIUrl":"10.1016/j.dental.2024.07.001","url":null,"abstract":"<div><h3>Objectives</h3><p>To evaluate the effect of different ratios of Bis-EMA/Bis-GMA resin mixtures on the inherent viscosity and curing-related properties: including degree of cure (DC%), shrinkage strain, Knoop micro-hardness (KH) and flexural strength of resin-impregnated fiber-bundles.</p></div><div><h3>Methods</h3><p>Bis-EMA/Bis-GMA monomers were mixed (by weight) in the following ratios: M1 = 30 %/70 %, M2 = 50 %/50 %, M3 = 70 %/30 %, and M4 = 100 %/0 %. Standard measurements were made of refractive index, viscosity, degree of conversion, shrinkage strain and Knoop hardness (KHN). For 60 % glass fiber-bundles impregnated with 40 % resin, three-point bending test for flexural strength and shrinkage strain were measured. Data were analyzed by One-way ANOVA and Bonferroni <em>post-hoc</em> tests (α = 0.05).</p></div><div><h3>Results</h3><p>For resin mixtures, increasing Bis-EMA proportion decreased refractive index (p &lt; 0.05), and viscosity (p &lt; 0.05), and increased monomer conversion (DC%), shrinkage strain and KHN (p &lt; 0.05). DC% increased after 1 h for all resin mixtures. The shrinkage strain and flexural strength of resin-impregnated fiber-bundles reduced with increased Bis-EMA.</p></div><div><h3>Significance</h3><p>Monomeric mixtures with highest amounts of Bis-EMA showed enhancement in several clinically-relevant properties and polymerization of respective resin-impregnated glass fibers. This makes them potential candidates for impregnating glass fibers in fiber-reinforced restorations.</p></div>","PeriodicalId":298,"journal":{"name":"Dental Materials","volume":"40 10","pages":"Pages 1652-1657"},"PeriodicalIF":4.6,"publicationDate":"2024-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141858525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis and challenges of fluorinated divinyl urethane monomers as a strategy for masking hydrolytic sensitive methacrylate groups in resin composites","authors":"Michael Lagowski ,&nbsp;Zach Gouveia ,&nbsp;Meilin Yang ,&nbsp;Yoav Finer ,&nbsp;J.Paul Santerre","doi":"10.1016/j.dental.2024.07.006","DOIUrl":"10.1016/j.dental.2024.07.006","url":null,"abstract":"<div><h3>Objective</h3><p>The biodegradation of methacrylate (MA)-based dental restoratives has been suggested to contribute to a loss of adhesion and subsequent detachment, or secondary caries, both major causes of restoration failure. Previous studies have demonstrated that intermolecular interactions between resin monomers may affect the hydrolytic-susceptibility of composites. Altering the intermolecular interactions by shielding or masking the hydrolytically-susceptible ester groups found in MA monomers could be an effective strategy to mitigate the biodegradation of resin composites. The objective of this work was to assess whether shielding/masking MAs using fluorinated groups could improve the biostability of experimental composites.</p></div><div><h3>Methods</h3><p>Eight fluorinated monomers (FM) were synthesized, characterized (¹H NMR), and formulated into experimental resin composites (FC, 65 wt%, microfill). FCs were assessed for interactions with water (water contact angle, water sorption, gel fraction), mechanical properties (both compressive and flexural strength and modulus), cytocompatibility, resistance to biodegradation using simulated human salivary esterase (SHSE) and compared to a control composite (CC) without FM.</p></div><div><h3>Results</h3><p>Integration of FMs was found to generally decrease both the physical and mechanical properties under all incubation conditions when compared to the CC. Additionally, all FCs had a negative influence on composite biodegradation following immersion in SHSE when compared to the CC.</p></div><div><h3>Significance</h3><p>Shielding/masking MA-esters inherently inserts molecular spaces between the polymer chains within the resin network, and shielding is likely not possible while also maintaining the necessary cohesive forces that regulate the physical and mechanical properties of resin composites. Novel dental resin development should seek to remove/replace vulnerable ester-containing MAs rather that adopting a shielding/masking approach.</p></div>","PeriodicalId":298,"journal":{"name":"Dental Materials","volume":"40 10","pages":"Pages 1624-1634"},"PeriodicalIF":4.6,"publicationDate":"2024-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0109564124002112/pdfft?md5=7e950bf045384d069b52612329097167&pid=1-s2.0-S0109564124002112-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141858527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Design and optimization of a novel patient-specific subperiosteal implant additively manufactured in yttria-stabilized zirconia","authors":"Gunpreet Oberoi ,&nbsp;Erik Kornfellner ,&nbsp;Daniel Alexander Aigner ,&nbsp;Ewald Unger ,&nbsp;Martin Schwentenwein ,&nbsp;Daniel Bomze ,&nbsp;Christoph Staudigl ,&nbsp;Dieter Pahr ,&nbsp;Francesco Moscato","doi":"10.1016/j.dental.2024.07.008","DOIUrl":"10.1016/j.dental.2024.07.008","url":null,"abstract":"<div><h3>Objective</h3><p>To design a patient-specific subperiosteal implant for a severely atrophic maxillary ridge using yttria-stabilized additively manufactured zirconia (3YSZ) and evaluate its material properties by applying topology optimization (TO) to replace bulk material with a lattice structure.</p></div><div><h3>Materials</h3><p>A contrast-based segmented skull model from anonymized computed tomography data of a patient was used for the initial anatomical design of the implant for the atrophic maxillary ridge. The implant underwent finite element analysis (FEA) and TO under different occlusal load-bearing conditions. The resulting implant designs, in bulk material and lattice, were evaluated via in-silico tensile tests and 3D printed.</p></div><div><h3>Results</h3><p>The workflow produced two patient-specific subperiosteal designs: a) an anatomically precise bulk implant, b) a TO lattice implant. In-silico tensile tests revealed that the Young’s modulus of yttria-stabilized zirconia is 205 GPa for the bulk material and 83.3 GPa for the lattice. Maximum principal stresses in the implant were 61.14 MPa in bulk material and 278.63 MPa in lattice, both tolerable, indicating the redesigned implant can withstand occlusal forces of 125–250 N per abutment. Furthermore, TO achieved a 13.10 % mass reduction and 208.71 % increased surface area, suggesting improved osteointegration potential.</p></div><div><h3>Significance</h3><p>The study demonstrates the planning and optimization of ceramic implant topology. A further iteration of the implant was successfully implanted in a patient-named use case, employing the same fabrication process and parameters.</p></div>","PeriodicalId":298,"journal":{"name":"Dental Materials","volume":"40 10","pages":"Pages 1568-1574"},"PeriodicalIF":4.6,"publicationDate":"2024-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0109564124002136/pdfft?md5=9e3ec24f1bfea8198f564ca1ab6d7e4c&pid=1-s2.0-S0109564124002136-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141858526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Conventionally and digitally fabricated removable complete dentures: manufacturing accuracy, fracture resistance and repairability","authors":"Adrian Zahel ,&nbsp;Ariadne Roehler ,&nbsp;Pablo Kaucher-Fernandez ,&nbsp;Sebastian Spintzyk ,&nbsp;Frank Rupp ,&nbsp;Eva Engel","doi":"10.1016/j.dental.2024.07.022","DOIUrl":"10.1016/j.dental.2024.07.022","url":null,"abstract":"<div><h3>Objectives</h3><p>Conventionally and digitally manufactured removable complete dentures with different dentition forms were examined for manufacturing accuracy (trueness, precision), fracture forces under torsional loading and subsequent repairability.</p></div><div><h3>Methods</h3><p>A total of 90 mandibular prostheses were manufactured. Ten were made using the injection molding technique and finished with prefabricated teeth. 40 bases each, were manufactured subtractively and additively. Digitally the prosthesis’ dental arch was divided either into two quadrants or three sextants, or kept as full arch. Afterwards, ten additive and subtractive bases were finished with prefabricated teeth and ten of each with milled quadrants, sextants and full arches. After manufacturing, all specimens were rescanned for accuracy comparisons using the Root Mean Square (RMS). Lastly, all specimens were tested to failure under torsional loading.</p></div><div><h3>Results</h3><p>Conventionally manufactured dentures showed the greatest deviation in accuracy. The type of base manufacturing did not determine the fracture resistance of the prostheses. The dentition form had a significant influence. While prefabricated teeth (86.01 ± 19.76 N) and quadrants (77.89 ± 9.58 N) showed a low fracture resistance, sextants (139.12 ± 21.41 N) and full arches (141.05 ± 17.14 N) achieved the highest fracture forces. Subtractive bases with prefabricated teeth or quadrants were assessed to be repairable, digital dentures with full arch were assessed as not repairable.</p></div><div><h3>Significance</h3><p>The presented testing set-up is suitable to determine the fracture behavior of dentures rather than of standards. With the possibility of digital design and individual manufacturing, dentures’ mechanical stability can be significantly increased, especially with suitable dentition forms.</p></div>","PeriodicalId":298,"journal":{"name":"Dental Materials","volume":"40 10","pages":"Pages 1635-1642"},"PeriodicalIF":4.6,"publicationDate":"2024-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0109564124002288/pdfft?md5=5bc51b594c5f29ed6a1d7b975d141994&pid=1-s2.0-S0109564124002288-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141854379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biaxial flexural strength of nanoglass and multiwalled carbon nanotubes reinforced 3D-printed denture base resins and their shear bond strength to 3D-printed and acrylic denture teeth","authors":"Yomna M. Ibrahim ,&nbsp;Pansai A. Mohamed ,&nbsp;Kenda I. Hanno ,&nbsp;Mohamed M. Abdul-Monem","doi":"10.1016/j.dental.2024.07.026","DOIUrl":"10.1016/j.dental.2024.07.026","url":null,"abstract":"<div><h3>Objective</h3><p>Evaluation of biaxial flexural strength (BFS) of nanoglass (NG) and multiwalled carbon nanotubes (MWCNTs) reinforced 3D-printed denture base resins and their shear bond strength (SBS) to 3D-printed and acrylic denture teeth.</p></div><div><h3>Methods</h3><p>Silanized NG and MWCNTs were added to 3D-printed denture base resin to obtain four groups: Control, 0.25 wt% NG, 0.25 wt% MWCNTs, and a combination group with 0.25 wt% of both fillers. All specimens were tested before and after 600 cycles of thermal aging. BFS (n = 88) was tested using disk-shaped specimens (12 ×2 mm) centralized on an O ring in a universal testing machine. Weibull analysis was conducted to assess predictability of failure. SBS (n = 176) was tested for acrylic and 3D-printed denture teeth attached to bar-shaped specimens in a universal testing machine followed by failure mode analysis using stereomicroscope. Two and three-way ANOVA tests followed by Tukey post hoc test were conducted for BFS and SBS. Kruskal-Wallis test compared percent change among groups with subsequent Dunn post hoc test with Bonferroni correction (α = 0.05).</p></div><div><h3>Results</h3><p>BFS was affected significantly by filler content (<em>P</em> &lt; 0.001) and thermal cycling (<em>P</em> &lt; 0.001), with thermal cycling displaying the uppermost effect (Ƞp² =0.551). A significant interaction between filler content, thermal cycling, and teeth type was displayed by SBS results (<em>P</em> &lt; 0.001, F=10.340, Ƞp² =0.162). The highest BFS values belonged to 0.25 % MWCNTs while the highest SBS to printed teeth was displayed by the combination.</p></div><div><h3>Significance</h3><p>The combination group displayed higher BFS and SBS to printed teeth compared to control which allows 3D-printed materials to have a long-term clinical success.</p></div>","PeriodicalId":298,"journal":{"name":"Dental Materials","volume":"40 10","pages":"Pages 1557-1567"},"PeriodicalIF":4.6,"publicationDate":"2024-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141854377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Monomer elution and shrinkage stress analysis of addition-fragmentation chain-transfer-modified resin composites in relation to the curing protocol","authors":"Donát Szebeni ,&nbsp;Roland Told ,&nbsp;Sándor Kunsági-Máté ,&nbsp;József Szalma ,&nbsp;Péter Maróti ,&nbsp;Katalin Böddi ,&nbsp;Edina Lempel","doi":"10.1016/j.dental.2024.07.029","DOIUrl":"10.1016/j.dental.2024.07.029","url":null,"abstract":"<div><h3>Objective</h3><p>The purpose was to compare the effects of rapid (3 s) and conventional (20 s) polymerization protocols (PP) of mono- and multichip LED curing units (LCU) on shrinkage stress (SS) and monomer elution (ME) in bulk-fill resin-based composites (RBC) with and without addition-fragmentation chain-transfer (AFCT) monomer.</p></div><div><h3>Methods</h3><p>Cylindrical (5x4mm) specimens were prepared from two RBCs containing different AFCT monomers (Filtek OneBulk-FOB; Tetric PowerFill-TPF) and one without (Tetric EvoCeram Bulk-TEC). After soaking for 3, 10, and 14 days (75 % ethanol), ME was quantified using standard monomers by High-Performance Liquid Chromatography. SS was measured from the start of polymerization to 5 min using a Materials Testing Machine. The radiant exitance of LCUs was measured using a spectrophotometer. ANOVA and Tukey's post-hoc test, multivariate analysis and partial eta-squared statistics were used to analyze the data (p &lt; 0.05).</p></div><div><h3>Results</h3><p>AFCT-modification significantly decreased ME (p &lt; 0.001). ME was reduced by half by day 10 and by one tenth by the end of the 14-day compared to the 3-day sampling. ME itself was dependent, whereas the percentage of monomers released was independent of the PP used (p &gt; 0.05). FOB showed the lowest SS (p &lt; 0.001), while there was no significant difference between TPF and TEC (p = 0.124). Both ME and SS were significantly influenced by material type and PP.</p></div><div><h3>Significance</h3><p>The incorporation of the AFCT monomer reduced ME, but this was inversely related to a decrease in exposure time. SS values reduced by rapid PP in parallel with increasing ME values. The utilization of the AFCT molecule in conjunction with an appropriate resin-, initiator-system is of significant consequence for the kinetics of polymerization and the incorporation of monomers into the network.</p></div>","PeriodicalId":298,"journal":{"name":"Dental Materials","volume":"40 10","pages":"Pages 1611-1623"},"PeriodicalIF":4.6,"publicationDate":"2024-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0109564124002318/pdfft?md5=870100ad6e2008ffd2c6f0ebe43c0d75&pid=1-s2.0-S0109564124002318-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141854381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Composition control of additively manufactured color-graded temporary veneer","authors":"Imam Akbar Sutejo ,&nbsp;Sinuo Zhang ,&nbsp;Chang Woo Gal ,&nbsp;Yeong-Jin Choi ,&nbsp;Honghyun Park ,&nbsp;Sungmin Kim ,&nbsp;Hyung-bong Lim ,&nbsp;Hui-suk Yun","doi":"10.1016/j.dental.2024.07.027","DOIUrl":"10.1016/j.dental.2024.07.027","url":null,"abstract":"<div><h3>Objective</h3><p>The aim of this study was to design and assess composite resin composition for patient-specific esthetic color-graded temporary veneer.</p></div><div><h3>Methods</h3><p>Various compositions of composite structures (assorted by Ba₂SiO₄ filler, TiO₂ pigment, and photoinitiator) were prepared via additive manufacturing with 3 s UV exposure (405 nm, 10 W/cm²) per 50 µm thick layer followed by 20 min post-curing treatment after fabrication. The effect of each component on the generated color shades was observed and compared to the commonly used VITA shade guide. The coloration was explored by staining aging treatment under dry, wet, artificial saliva environments, coffee, and cola. The mechanical properties were also evaluated. Color measurement and comparison were done using a colorimeter (lightness (L*), green-red color (a*), and blue-yellow color (b*)), and the changes were calculated by CIEDE2000 (ΔE₀₀), translucency parameter (TP) and whiteness index (WI_D). The composition color analysis results were then applied to produce a color-graded temporary veneer for mimicking a natural look.</p></div><div><h3>Result</h3><p>Mechanically, all composition result in adequate bending strength with maximum achievable strength of 111.64 MPa. At the same time, the composite color was affected by each constituent differently. The L* value, which indicates the color lightness of the composite, was considerably tuned by the TiO₂ pigment, whereas Ba₂SiO₄ filler only triggered minor changes. Photoinitiator concentration significantly affected the yellowness, indicated by the increased b* value. Similar tendency also observed toward the calculated TP and WI_D as well. Based on these evaluations, color-graded temporary veneer successfully generated, matching the VITA A3, A2, and B1 shades gradation. However, the stability of the composite color decreased at high amounts of Ba₂SiO₄ and photoinitiator.</p></div><div><h3>Significance</h3><p>The study presents a composition guide for fabricating temporary patient-specific color-graded veneer. It provides insights on the effect of the constituent material on dental esthetics.</p></div>","PeriodicalId":298,"journal":{"name":"Dental Materials","volume":"40 10","pages":"Pages 1643-1651"},"PeriodicalIF":4.6,"publicationDate":"2024-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0109564124002264/pdfft?md5=c8d8d2cc9164e133fa01dc09f7e24ee0&pid=1-s2.0-S0109564124002264-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141854378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Load-bearing capacity, internal accuracy and time-efficiency of heat-pressed, milled and 3D-printed lithium disilicate ultra-thin occlusal veneers","authors":"Pune Nina Paqué ,&nbsp;Cindy Gantner ,&nbsp;Kiren Jan Mätzener ,&nbsp;Mutlu Özcan ,&nbsp;Alexis Ioannidis","doi":"10.1016/j.dental.2024.07.017","DOIUrl":"10.1016/j.dental.2024.07.017","url":null,"abstract":"<div><h3>Objectives</h3><p>The primary aim of this in vitro study was to compare the load-bearing capacity of lithium disilicate occlusal veneers, fabricated via different manufacturing processes. Secondary objectives included assessing internal accuracy and production time-efficiency.</p></div><div><h3>Methods</h3><p>Four fabrication methods for ultra-thin lithium disilicate occlusal veneers on extracted human molars with simulated erosive defects were compared (n = 20/group): CAM: milled lithium disilicate (IPS e.max CAD); HPR: heat-pressed lithium disilicate (IPS e.max Press) out of a milled PMMA template (Ddpmma CAST); 3DP: 3D-printed lithium disilicate (experimental lithium disilicate); PTE: heat-pressed lithium disilicate (IPS e.max Press) out of a 3D-printed template (SilaPrint cast). Internal accuracy was measured prior to thermo-mechanical aging, followed by static loading to measure the load-bearing capacity (F_max). Fabrication time (time-efficiency) was also recorded. Statistical analysis was performed using the Kruskal-Wallis (KW) test.</p></div><div><h3>Results</h3><p>No statistically significant differences were found in median load-bearing capacities (F_max) between the groups (KW p = 0.5902): CAM 1821 N, HPR 1896 N, 3DP 2003 N, PTE 1687 N. Significant differences were found in internal accuracy between the groups that employed printing processes (3DP, PTE) and all other groups in margins (p &lt; 0.001), cusps (p &lt; 0.0018), and fossae (p &lt; 0.0346). The time-efficiency measurements indicated an increase in fabrication time, starting from CAM 67.2 ± 5.8 min, followed by HPR 200.8 ± 33.0 min, PTE 289.2 ± 38.7 min, and peaking with the highest duration observed for 3DP 701.6 ± 8.1 min.</p></div><div><h3>Significance</h3><p>The fabrication method of ultra-thin lithium disilicate occlusal veneers does not significantly impact their load-bearing capacity, but affects the clinical fit and adaptation of the veneers.</p></div>","PeriodicalId":298,"journal":{"name":"Dental Materials","volume":"40 10","pages":"Pages 1602-1610"},"PeriodicalIF":4.6,"publicationDate":"2024-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0109564124002070/pdfft?md5=d549af4f732ce30a8b602828f9e3f13a&pid=1-s2.0-S0109564124002070-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141854380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mitigating lipopolysaccharide-induced impairment in human dental pulp stem cells with tideglusib-doped nanoparticles: Enhancing osteogenic differentiation and mineralization","authors":"Raquel Osorio ,&nbsp;Francisco J. Rodríguez-Lozano ,&nbsp;Manuel Toledano ,&nbsp;Manuel Toledano-Osorio ,&nbsp;David García-Bernal ,&nbsp;Laura Murcia ,&nbsp;Sergio López-García","doi":"10.1016/j.dental.2024.07.012","DOIUrl":"10.1016/j.dental.2024.07.012","url":null,"abstract":"<div><h3>Objective</h3><p>Drug-loaded non-resorbable polymeric nanoparticles (NPs) are proposed as an adjunctive treatment for pulp regenerative strategies. The present in vitro investigation aimed to evaluate the effectiveness of tideglusib-doped nanoparticles (TDg-NPs) in mitigating the adverse effects of bacterial lipopolysaccharide endotoxin (LPS) on the viability, morphology, migration, differentiation and mineralization potential of human dental pulp stem cells (hDPSCs).</p></div><div><h3>Methods</h3><p>Cell viability, proliferation, and differentiation were assessed using a MTT assay, cell migration evaluation, cell cytoskeleton staining analysis, Alizarin Red S staining and expression of the odontogenic related genes by a real-time quantitative polymerase chain reaction (RT-qPCR) were also performed. Cells were tested both with and without stimulation with LPS at various time points. One-way ANOVA and Tukey's test were employed for statistical analysis (<em>p</em> &lt; 0.05).</p></div><div><h3>Results</h3><p>Adequate cell viability was encountered in all groups and at every tested time point (24, 48, 72 and 168 h), without differences among the groups (<em>p</em> &gt; 0.05). The analysis of cell cytoskeleton showed nuclear alteration in cultures with undoped NPs after LPS stimulation. These cells exhibited an in blue diffuse and multifocal appearance. Some nuclei looked fragmented and condensed. hDPSCs after LPS stimulation but in the presence of TDg-NPs exhibited less nuclei changes. LPS induced down-regulation of Alkaline phosphatase, Osteonectin and Collagen1 gene markers, after 21d. LPS half-reduced the cells production of calcium deposits in all groups (<em>p</em> &lt; 0.05), except in the group with TDg-NPs (decrease about 10 %).</p></div><div><h3>Significance</h3><p>LPS induced lower mineral deposition and cytoskeletal disorganization in hDPSCs. These effects were counteracted by TDg-NPs, enhancing osteogenic differentiation and mineralization.</p></div>","PeriodicalId":298,"journal":{"name":"Dental Materials","volume":"40 10","pages":"Pages 1591-1601"},"PeriodicalIF":4.6,"publicationDate":"2024-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141786670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanical properties and crown accuracy of additively manufactured zirconia restorations","authors":"Sae-Eun Oh ,&nbsp;Ji-Man Park ,&nbsp;Jee-Hwan Kim ,&nbsp;June-Sung Shim ,&nbsp;Young-Bum Park","doi":"10.1016/j.dental.2024.07.016","DOIUrl":"10.1016/j.dental.2024.07.016","url":null,"abstract":"<div><h3>Objectives</h3><p>We evaluated the mechanical properties of zirconia restorations produced via additive manufacturing (AM) and the crown accuracy of zirconia crowns.</p></div><div><h3>Methods</h3><p>Zirconia disks, bars, and crowns were manufactured via subtractive (CNC group) and additive manufacturing (AM group) techniques. Disk-shaped specimens in each group were autoclaved at 134 °C and 216 kPa for 5, 10, and 24 h. The phases of the specimens were analyzed using an X-ray diffractometer. The flexural strengths were measured via biaxial flexural tests. The morphologies were examined using a scanning electron microscope. The correlation between the m-phase fraction and biaxial flexural strength by autoclave time in each group was analyzed via linear mixed model and Pearson’s correlation analysis. For each group, crown specimens were used to assess the marginal and internal gaps using the replica technique. Buccolingual and mesiodistal cross-sections were measured, and a repeated measures one-way ANOVA was performed.</p></div><div><h3>Results</h3><p>Linear mixed model analysis indicated that for both groups, with an increase in the autoclave time, the flexural strength decreased, whereas the m-phase fraction increased. Pearson’s correlation analysis revealed no correlation between the m-phase fraction and flexural strength for either group. A repeated measures one-way ANOVA was conducted on instrumented sections (buccal, lingual, mesial, and distal), revealing that the marginal and internal gaps of AM-produced zirconia crowns were less accurate than those of CNC-produced zirconia crowns.</p></div><div><h3>Significance</h3><p>These findings suggest that additively produced zirconia restorations have mechanical properties comparable to those of conventionally produced ceramics and may be suitable for clinical applications.</p></div>","PeriodicalId":298,"journal":{"name":"Dental Materials","volume":"40 10","pages":"Pages 1546-1556"},"PeriodicalIF":4.6,"publicationDate":"2024-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141764599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}