Dental Materials最新文献_第9页

Bifunctional naringenin-laden gelatin methacryloyl scaffolds with osteogenic and anti-inflammatory properties 具有成骨和抗炎特性的双功能柚皮苷明胶甲基丙烯酰支架。

IF 4.6 1区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Dental Materials

Pub Date : 2024-06-14 DOI: 10.1016/j.dental.2024.06.019

Objective

To fabricate and characterize an innovative gelatin methacryloyl/GelMA electrospun scaffold containing the citrus flavonoid naringenin/NA with osteogenic and anti-inflammatory properties.

Methods

GelMA scaffolds (15 % w/v) containing 0/Control, 5, 10, or 20 % of NA w/w were obtained via electrospinning. The chemical composition, fiber morphology/diameter, swelling/degradation profile, and NA release were investigated. Cytotoxicity, cell proliferation, adhesion and spreading, total protein/TP production, alkaline phosphatase/ALP activity, osteogenic genes expression (OCN, OPN, RUNX2), and mineralized nodules deposition/MND with human alveolar bone-derived mesenchymal stem cells (aBMSCs) seeded on the scaffolds were assessed. Moreover, aBMSCs seeded on the scaffolds and stimulated with tumor necrosis factor-alpha/TNF-α were submitted to collagen, nitric oxide/NO, interleukin/IL-1α, and IL-6 production assessment. Data were analyzed using ANOVA and t-student/post-hoc tests (α = 5 %).

Results

NA-laden scaffolds presented increased fiber diameter, lower swelling capacity, and faster degradation profile over 28 days (p < 0.05). NA release was detected over time. Cell adhesion and spreading, and TP production were similar between GelMA and GelMA+NA5 % scaffolds, while cell proliferation, ALP activity, OCN/OPN/RUNX2 gene expression, and MND were higher for GelMA+NA5 % scaffolds (p < 0.05). Cells seeded on control scaffolds and TNF-α-stimulated presented higher levels of NO, IL-1α/IL-6, and lower levels of collagen (p < 0.05). In contrast, cells seeded on GelMA+NA5 % scaffolds showed downregulation of inflammatory markers and higher collagen synthesis (p < 0.05).

Significance

GelMA+NA5 % scaffold was cytocompatible, stimulated aBMSCs proliferation and differentiation, and downregulated inflammatory mediators’ synthesis, suggesting its therapeutic effect as a multi-target bifunctional scaffold with osteogenic and anti-inflammatory properties for bone tissue engineering.

目的制备并表征一种含有柑橘类黄酮柚皮苷/NA的创新性明胶甲基丙烯酰/凝胶MA电纺支架，该支架具有成骨和抗炎特性：方法：通过电纺丝获得含 0/对照组、5%、10% 或 20%柚皮苷/NA（重量比为 15%）的 GelMA 支架（重量比为 15%）。研究了化学成分、纤维形态/直径、膨胀/降解曲线和 NA 释放情况。评估了细胞毒性、细胞增殖、粘附和扩散、总蛋白/TP 生成、碱性磷酸酶/ALP 活性、成骨基因表达（OCN、OPN、RUNX2）以及播种在支架上的人肺泡骨源性间充质干细胞（aBMSCs）的矿化结节沉积/MND。此外，在支架上播种并接受肿瘤坏死因子-α/TNF-α刺激的间充质干细胞还接受了胶原蛋白、一氧化氮/NO、白细胞介素/IL-1α和IL-6产生情况的评估。数据分析采用方差分析和 t-学生/事后检验（α = 5 %）：结果：NA负载的支架在28天内纤维直径增大，膨胀能力降低，降解速度加快（p显著性：GelMA+NA5 %支架具有细胞相容性，能刺激aBMSCs增殖和分化，并能降低炎症介质的合成，这表明它是一种多靶点双功能支架，具有成骨和抗炎特性，可用于骨组织工程。

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引用次数: 0

Effects of 10-MDP calcium salt on osteoblasts and fibroblasts 10-MDP 钙盐对成骨细胞和成纤维细胞的影响

IF 4.6 1区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Dental Materials

Pub Date : 2024-06-14 DOI: 10.1016/j.dental.2024.06.015

Objective

10-methacryloyloxidecyl dihydrogen phosphate monomer (10-MDP) is commonly used as a bonding monomer in universal adhesives. Adhesives that contain this monomer can directly contact the surrounding periodontium due to the chemical binding of 10-MDP with hydroxyapatite in hard tissue to form calcium salts. However, the effect of these calcium salts on the periodontium in the case of subgingival fillings remains poorly understood. The objective of this study was to investigate effects of 10-MDP calcium salts on osteoblasts and fibroblasts in the periodontal tissues.

Methods

This study investigated the effects of different concentrations of 10-MDP calcium salts on the migration, proliferation, and differentiation of osteoblasts (MC3T3-E1) and fibroblasts (L929); additionally, the effect on apoptosis and matrix metalloproteinases (MMPs) expression in these cells was evaluated. Cell proliferation assay, alkaline phosphatase (ALP) activity assay, Western blotting, and quantitative real-time polymerase chain reaction were performed to determine the effects.

Results

The 10-MDP calcium salts (within a concentration of 0.5 mg/mL) showed no cytotoxicity and did not seem to influence the apoptosis, mitochondrial membrane potential, and reactive oxygen species (ROS) levels in the cells. However, they had an inhibitory effect on the secretion of MMP2 and MMP9 in the osteoblasts and fibroblasts. The ALP activity assay and Alizarin Red staining did not reveal any significant effects of the 10-MDP calcium salts on osteoblast differentiation.

Significance

These results suggest that applying 10-MDP-containing adhesives to subgingival fillings may be safe and beneficial for the periodontal tissues.

目的：10-甲基丙烯酰氧癸基磷酸二氢酯单体（10-MDP）通常用作通用粘合剂的粘合单体。由于 10-MDP 与硬组织中的羟基磷灰石化学结合形成钙盐，因此含有这种单体的粘合剂可直接接触周围的牙周。然而，这些钙盐对龈下充填物牙周的影响仍然知之甚少。本研究的目的是调查 10-MDP 钙盐对牙周组织中成骨细胞和成纤维细胞的影响：本研究调查了不同浓度的 10-MDP 钙盐对成骨细胞（MC3T3-E1）和成纤维细胞（L929）迁移、增殖和分化的影响；此外，还评估了对这些细胞凋亡和基质金属蛋白酶（MMPs）表达的影响。为了确定这些影响，还进行了细胞增殖测定、碱性磷酸酶（ALP）活性测定、Western 印迹和实时定量聚合酶链反应：10-MDP 钙盐（浓度在 0.5 毫克/毫升以内）没有细胞毒性，似乎也不会影响细胞凋亡、线粒体膜电位和活性氧（ROS）水平。不过，它们对成骨细胞和成纤维细胞中 MMP2 和 MMP9 的分泌有抑制作用。ALP 活性测定和茜素红染色并未显示 10-MDP 钙盐对成骨细胞分化有任何显著影响：这些结果表明，在龈下填料中使用含 10-MDP 的粘合剂可能是安全的，而且对牙周组织有益。

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引用次数: 0

Optimization of an ultra-bright real-time high-throughput renilla luciferase assay for antibacterial assessment of Streptococcus mutans biofilms 优化用于变异链球菌生物膜抗菌评估的超亮实时高通量雷尼拉荧光素酶测定。

IF 4.6 1区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Dental Materials

Pub Date : 2024-06-13 DOI: 10.1016/j.dental.2024.06.012

Objective

The present work demonstrates the optimization of a renilla-based real-time, ultra-bright, non-disruptive, high-throughput bioluminescence assay (HTS) to assess the metabolism of intact Streptococcus mutans biofilms and its utility in screening the antibacterial efficacy of experimental nanofilled dental adhesive resins containing varying concentrations of nitrogen-doped titanium dioxide nanoparticles (N_TiO₂). Methods. Optimization of the assay was achieved by screening real-time bioluminescence changes in intact Streptococcus mutans biofilms imposed by the various experimental biofilm growth parameters investigated (bacterial strain, growth media, sucrose concentration, dilution factor, and inoculum volume). The optimized assay was then used to characterize the antibacterial efficacy of experimental nanofilled dental adhesive resins. The assay’s ability to discriminate between bacteriostatic and bactericidal approaches was also investigated. Results. Relative Light Units (RLU) values from the HTS optimization were analyzed by multivariate ANOVA (α = 0.05) and coefficients of variation. An optimized HTS bioluminescence assay was developed displaying RLUs values (brightness) that are much more intense when comparing to other previously reported bioluminescence assays, thereby decreasing the error associated with bioluminescence assays and displaying better utility while investigating the functionalities of antimicrobial nanofilled experimental dental adhesive resins with proven long-term properties. Significance. The present study is anticipated to positively impact subsequent research on dental materials and oral microbiology because it serves as a valuable screening tool in metabolic-based assays with increased sensitivity and robustness. The assay reported is anticipated to be further optimized to be used as a co-reporter for other Luc based assays.

研究目的本研究展示了一种基于雷尼拉的实时、超亮、无干扰、高通量生物发光检测方法（HTS）的优化，该方法可用于评估完整变异链球菌生物膜的新陈代谢，并可用于筛选含有不同浓度氮掺杂二氧化钛纳米颗粒（N_TiO2）的实验性纳米填充牙科粘合剂树脂的抗菌功效：方法：通过筛选各种实验性生物膜生长参数（细菌菌株、生长介质、蔗糖浓度、稀释因子和接种量）对完整的变异链球菌生物膜产生的实时生物发光变化，对该检测方法进行了优化。优化后的检测方法被用于鉴定实验性纳米填充牙科粘合剂树脂的抗菌功效。此外，还研究了该测定法区分抑菌和杀菌方法的能力：通过多元方差分析（α = 0.05）和变异系数分析了 HTS 优化的相对光单位（RLU）值。优化后的 HTS 生物荧光测定法显示出的 RLU 值（亮度）与之前报道的其他生物荧光测定法相比更加强烈，从而降低了生物荧光测定法的相关误差，在研究具有经证实的长期特性的抗菌纳米填充实验性牙科粘合剂树脂的功能时显示出更好的实用性：本研究预计将对牙科材料和口腔微生物学的后续研究产生积极影响，因为它在基于代谢的检测中是一种宝贵的筛选工具，具有更高的灵敏度和稳健性。预计报告的检测方法将进一步优化，以用作其他基于 Luc 的检测方法的共同报告器。

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引用次数: 0

Transdentinal effects of S-PRG fillers on odontoblast-like cells S-PRG 填充剂对牙本质样细胞的横向影响

IF 4.6 1区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Dental Materials

Pub Date : 2024-06-13 DOI: 10.1016/j.dental.2024.06.002

Objectives

To investigate the transdentinal effects of surface reaction-type pre-reacted glass-ionomer (S-PRG) fillers on odontoblast-like cells.

Methods

An eluate of S-PRG fillers was obtained by dissolving the particles in distilled water (1:1 m/v). Dentin discs with similar permeability were mounted into artificial pulp chambers and MDPC-23 cells were seeded on their pulpal surface. The occlusal surface was treated with (n = 10): ultrapure water (negative control – NC), hydrogen peroxide (positive control – PC), S-PRG eluate exposure for 1 min (S-PRG 1 min), or S-PRG filler eluate exposure for 30 min (S-PRG 30 min). After 24 h, cell viability (alamarBlue) and morphology (SEM) were evaluated. The extract obtained from transdentinal diffusion was applied to MDPC-23 pre-cultured in plates for another 24 h to evaluate viability (alamarBlue, 1, 3, and 7 days), gene expression of Col1a1, Alpl, Dspp, and Dmp1 (RT-qPCR, 1 and 7 days), and mineralization (Alizarin Red, 7 days). Data were analyzed with ANOVA (α = 5 %).

Results

While S-PRG 1 min did not differ from NC, S-PRG 30 min reduced 17.9 % viability of cells from discs. S-PRG treatments resulted in low cell detaching from dentin, and the remaining cells exhibited typical morphology or minor cytoplasmic contraction. S-PRG 30 min slightly increased cell viability (6 %) 1 day after contact with the extract. S-PRG treatments upregulated the expression of the investigated genes, especially after 1 day. S-PRG 30 min stimulated mineralization activity by 39.7 %.

Conclusions

S-PRG filler eluate does not cause transdentinal cytotoxicity on odontoblast-like cells, and long-term exposure can stimulate their dentinogenic-related mineralization activity.

Significance

The transdentinal elution of ions from S-PRG fillers is not expected to be harmful to the dental pulp and may exert bioactive effects by inducing dentin matrix deposition through the metabolism of underlying odontoblasts.

目的研究表面反应型预反应玻璃-离子体（S-PRG）填料对骨母细胞样细胞的横向作用：方法：将 S-PRG 填充剂溶解在蒸馏水（1:1 m/v）中，得到洗脱液。将具有相似渗透性的牙本质盘装入人工牙髓腔，并在其牙髓表面播种 MDPC-23 细胞。对咬合面进行以下处理（n = 10）：超纯水（阴性对照 - NC）、过氧化氢（阳性对照 - PC）、S-PRG 洗脱液暴露 1 分钟（S-PRG 1 分钟）或 S-PRG 填充剂洗脱液暴露 30 分钟（S-PRG 30 分钟）。24 小时后，对细胞存活率（α-amarBlue）和形态（SEM）进行评估。将经齿孔扩散获得的洗脱液涂抹到预先培养在平板中的 MDPC-23 上 24 小时后，评估细胞活力（α-蓝，1、3 和 7 天）、Col1a1、Alpl、Dspp 和 Dmp1 的基因表达（RT-qPCR，1 和 7 天）和矿化（茜素红，7 天）。数据采用方差分析（α = 5 %）：虽然 S-PRG 1 分钟与 NC 没有差异，但 S-PRG 30 分钟却降低了 17.9 % 的圆盘细胞存活率。S-PRG 处理导致较少细胞从牙本质上脱离，剩余细胞表现出典型的形态或轻微的细胞质收缩。与提取物接触一天后，S-PRG 30 分钟可略微提高细胞存活率（6%）。S-PRG 处理可上调所研究基因的表达，尤其是在 1 天之后。S-PRG 30 分钟刺激了 39.7 % 的矿化活性：结论：S-PRG 填充剂洗脱液不会对牙本质母细胞造成经牙本质细胞毒性，长期接触可刺激牙本质相关矿化活性：意义：S-PRG 填充剂中的离子经牙髓洗脱预计不会对牙髓造成危害，并可能通过下层牙本质母细胞的新陈代谢诱导牙本质基质沉积，从而发挥生物活性作用。

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引用次数: 0

Advancing dentin remineralization: Exploring amorphous calcium phosphate and its stabilizers in biomimetic approaches 推进牙本质再矿化：探索生物仿生方法中的无定形磷酸钙及其稳定剂。

IF 4.6 1区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Dental Materials

Pub Date : 2024-06-13 DOI: 10.1016/j.dental.2024.06.013

Objective

This review elucidates the mechanisms underpinning intrafibrillar mineralization, examines various amorphous calcium phosphate (ACP) stabilizers employed in dentin’s intrafibrillar mineralization, and addresses the challenges encountered in clinical applications of ACP-based bioactive materials.

Methods

The literature search for this review was conducted using three electronic databases: PubMed, Web of Science, and Google Scholar, with specific keywords. Articles were selected based on inclusion and exclusion criteria, allowing for a detailed examination and summary of current research on dentin remineralization facilitated by ACP under the influence of various types of stabilizers.

Results

This review underscores the latest advancements in the role of ACP in promoting dentin remineralization, particularly intrafibrillar mineralization, under the regulation of various stabilizers. These stabilizers predominantly comprise non-collagenous proteins, their analogs, and polymers. Despite the diversity of stabilizers, the mechanisms they employ to enhance intrafibrillar remineralization are found to be interrelated, indicating multiple driving forces behind this process. However, challenges remain in effectively designing clinically viable products using stabilized ACP and maximizing intrafibrillar mineralization with limited materials in practical applications.

Significance

The role of ACP in remineralization has gained significant attention in dental research, with substantial progress made in the study of dentin biomimetic mineralization. Given ACP’s instability without additives, the presence of ACP stabilizers is crucial for achieving in vitro intrafibrillar mineralization. However, there is a lack of comprehensive and exhaustive reviews on ACP bioactive materials under the regulation of stabilizers. A detailed summary of these stabilizers is also instrumental in better understanding the complex process of intrafibrillar mineralization. Compared to traditional remineralization methods, bioactive materials capable of regulating ACP stability and controlling release demonstrate immense potential in enhancing clinical treatment standards.

目的：本综述阐明了牙本质纤维内矿化的基本机制，研究了牙本质纤维内矿化过程中使用的各种无定形磷酸钙（ACP）稳定剂，并探讨了基于ACP的生物活性材料在临床应用中遇到的挑战：本综述使用三个电子数据库进行文献检索：方法：使用 PubMed、Web of Science 和 Google Scholar 三个电子数据库，以特定关键词进行文献检索。根据纳入和排除标准对文章进行了筛选，以便对当前关于 ACP 在各类稳定剂影响下促进牙本质再矿化的研究进行详细的检查和总结：本综述强调了在各种稳定剂的调节下，ACP 在促进牙本质再矿化，特别是纤维内矿化方面所起作用的最新进展。这些稳定剂主要包括非胶原蛋白、其类似物和聚合物。尽管稳定剂种类繁多，但它们增强纤维内再矿化的机制却相互关联，这表明这一过程背后存在多种驱动力。然而，在实际应用中，如何使用稳定的 ACP 有效设计出临床上可行的产品，以及如何使用有限的材料最大限度地提高纤维内矿化度，仍然存在挑战：意义：ACP 在再矿化中的作用在牙科研究中获得了极大关注，牙本质生物仿生矿化研究也取得了重大进展。鉴于 ACP 在没有添加剂的情况下很不稳定，因此 ACP 稳定剂的存在对于实现体外纤维内矿化至关重要。然而，在稳定剂的规范下，缺乏对 ACP 生物活性材料的全面而详尽的评述。对这些稳定剂的详细总结也有助于更好地理解纤维内矿化的复杂过程。与传统的再矿化方法相比，能够调节 ACP 稳定性和控制释放的生物活性材料在提高临床治疗水平方面具有巨大的潜力。

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引用次数: 0

Evaluating antimicrobial, cytotoxic and immunomodulatory effects of glass ionomer cement modified by chitosan and hydroxyapatite 评估壳聚糖和羟基磷灰石改性的玻璃离子水泥的抗菌、细胞毒性和免疫调节作用。

IF 4.6 1区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Dental Materials

Pub Date : 2024-06-13 DOI: 10.1016/j.dental.2024.05.021

Objectives

This study aimed to assess antimicrobial efficacy, cytotoxicity, and cytokine release (IL-1b, IL-6, IL-10, TNF-α) from human dental pulp stem cells (hDPSCs) of chitosan (CH) and hydroxyapatite (HAp)-modified glass ionomer cements (GIC).

Methods

GICs with varied CH and HAp concentrations (0 %, 0.16 %, 2 %, 5 %, 10 %) were tested against S. mutans for 24 h or 7 days. Antimicrobial activity was measured using an MTT test. Cytotoxicity evaluation followed for optimal concentrations, analyzing mitochondrial activity and apoptosis in hDPSCs. Cytokine release was assessed with MAGPIX. Antimicrobial analysis used Shapiro-Wilk, Kruskal-Wallis, and Dunnett tests. Two-way ANOVA, Tukey, and Dunnett tests were applied for hDP metabolism and cytokine release.

Results

CH 2 % and HAp 5 % significantly enhanced GIC antimicrobial activity, especially after seven days. In immediate analysis, all materials showed reduced mitochondrial activity compared to the control. After 24 h, CH demonstrated mitochondrial metabolism similar to the control. All groups exhibited mild cytotoxicity (∼30 % cell death). Only IL-6 was influenced, with reduced release in experimental groups.

Significance

CH 2 % and HAp 5 % were most effective for antibacterial effects. GIC-CH 2 % emerged as the most promising formula, displaying significant antibacterial effects with reduced hDPSC toxicity.

研究目的本研究旨在评估壳聚糖（CH）和羟基磷灰石（HAp）改性的玻璃离子聚合物水门汀（GIC）对人牙髓干细胞（hDPSCs）的抗菌效果、细胞毒性和细胞因子释放（IL-1b、IL-6、IL-10、TNF-α）：方法：对不同浓度（0%、0.16%、2%、5%、10%）的壳聚糖和羟基磷灰石改性玻璃离子聚合物水门汀（GIC）进行 24 小时或 7 天的抗突变球菌测试。抗菌活性采用 MTT 试验进行测量。细胞毒性评估采用最佳浓度，分析线粒体活性和 hDPSCs 细胞凋亡。细胞因子释放用 MAGPIX 进行评估。抗菌分析采用 Shapiro-Wilk、Kruskal-Wallis 和 Dunnett 检验。对 hDP 代谢和细胞因子释放采用了双向方差分析、Tukey 和 Dunnett 检验：CH 2 % 和 HAp 5 % 显著增强了 GIC 的抗菌活性，尤其是在七天之后。在即时分析中，与对照组相比，所有材料都显示线粒体活性降低。24 小时后，CH 的线粒体代谢与对照组相似。所有组别都表现出轻微的细胞毒性（细胞死亡率为 30%）。只有 IL-6 受到影响，实验组的 IL-6 释放量减少：意义：CH 2 % 和 HAp 5 % 的抗菌效果最好。GIC-CH 2 % 是最有前景的配方，它具有显著的抗菌效果，同时降低了 hDPSC 的毒性。

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引用次数: 0

Functionally graded bi-material interface for Porcelain Veneered Zirconia dental crowns: A study using viscoelastic finite element analysis 瓷贴面氧化锆牙冠的功能分级双材料界面：粘弹性有限元分析研究。

IF 4.6 1区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Dental Materials

Pub Date : 2024-06-13 DOI: 10.1016/j.dental.2024.06.007

Objectives

During the manufacturing of Porcelain Veneered Zirconia (PVZ) dental crowns, the veneer-core system undergoes high-temperature firing cycles and gets fused together which is then, under a controlled setting, cooled down to room temperature. During this cooling process, the mismatch in thermal properties between zirconia and porcelain leads to the development of transient and residual thermal stresses within the crown. These thermal stresses are inherent to the PVZ dental crown systems and render the crown structure weak, acting as a precursor to veneer chipping, fracture, and delamination. In this study, the introduction of an intermediate functionally graded material (FGM) layer at the bi-material interface is investigated as a potentially viable alternative for providing a smoother transition of properties between zirconia and porcelain in a PVZ crown system.

Methods

Anatomically correct 3D crown models were developed for this study, with and without the FGM layer modeled at the bi-material interface. A viscoelastic finite element model was developed and validated for an anatomically correct bilayer PVZ crown system which was then used for predicting residual and transient stresses in the bilayer PVZ crown. Subsequently, the viscoelastic finite element model was further extended for the analysis of graded sublayers within the FGM layer, and this extended model was used for predicting the residual and transient stresses in the functionally graded PVZ crown, with an FGM layer at the bi-material interface.

Results

The study showed that the introduction of an FGM layer at the bi-material interface has the potential to reduce the effects from transient and residual stresses within the PVZ crown system relative to a bilayer PVZ crown structure. Furthermore, the study revealed that the FGM layer causes stress redistribution to alleviate the stress concentration at the interfacial surface between porcelain and zirconia which can potentially enhance the durability of the PVZ crowns towards interfacial debonding or fracture.

Significance

Thus, the use of an FGM layer at the bi-material interface shows a good prospect for enhancing the longevity of the PVZ dental crown restorations by alleviating the abrupt thermal property difference and relaxing thermal stresses.

目标：在制造瓷饰面氧化锆（PVZ）牙冠的过程中，饰面-核心系统要经过高温烧制循环并熔合在一起，然后在受控环境下冷却到室温。在冷却过程中，氧化锆和瓷之间热性能的不匹配会导致牙冠内部产生瞬时和残余热应力。这些热应力是 PVZ 牙冠系统所固有的，会使牙冠结构变得脆弱，成为贴面崩裂、断裂和分层的前兆。本研究探讨了在双材料界面上引入功能分级材料（FGM）中间层，作为一种潜在的可行替代方法，在 PVZ 牙冠系统中实现氧化锆和瓷之间更平滑的特性过渡：本研究开发了解剖正确的三维牙冠模型，在双材料界面上分别建模了和未建模 FGM 层。针对解剖正确的双层 PVZ 牙冠系统开发并验证了粘弹性有限元模型，然后用于预测双层 PVZ 牙冠中的残余应力和瞬态应力。随后，对粘弹性有限元模型进行了进一步扩展，以分析 FGM 层内的分级子层，并利用该扩展模型预测了在双材料界面上具有 FGM 层的功能分级 PVZ 牙冠的残余应力和瞬态应力：研究结果表明，与双层 PVZ 冠结构相比，在双材料界面上引入 FGM 层有可能减少 PVZ 冠系统中的瞬态应力和残余应力的影响。此外，研究还发现 FGM 层会导致应力重新分布，从而减轻瓷和氧化锆界面表面的应力集中，这有可能提高 PVZ 牙冠的耐久性，防止界面脱粘或断裂：因此，在双材料界面使用 FGM 层可缓解突然出现的热性能差异和热应力，从而提高 PVZ 牙冠修复体的使用寿命。

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引用次数: 0

Proposing new standards for testing solubility of pulp preservation materials 提出纸浆防腐材料溶解性测试的新标准。

IF 4.6 1区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Dental Materials

Pub Date : 2024-06-13 DOI: 10.1016/j.dental.2024.05.028

Objectives

Quality control testing of dental materials requires a standard to enable the generation of reproducible and comparable data. Currently there are no standards for testing materials used for vital pulp therapy. The aim of this study was to develop a new standard to evaluate solubility of pulp preservation materials.

Methods

The solubility of three materials used for vital pulp therapy: Biodentine, TheraCal and Activa was evaluated using two international standards for dental materials ISO 4049:2019 (S1) and ISO 6876:2012 (S2). For both standards, a modified methodology was evaluated. This included changing the volume of the solution used (S1M, S2M), using Dulbecco’s modified eagle medium (DMEM) as an alternative to water (S1D, S2D) and periodic solution change for the ISO 4049 method (S1P, S1MP). Materials were characterised before and after completion of solubility test using scanning electron microscopy (SEM) and X-ray diffraction (XRD) analysis.

Results

The test materials exhibited different solubility values depending on the methodology used. Biodentine exhibited significantly lower solubility when lower volumes of solution were used when tested using both ISO methods (p ≤ 0.05). TheraCal and Activa showed negative solubility values after desiccation when tested using ISO 4049:2019. The Biodentine exhibited changes in its microstructure which was dependent on the method used to test solubility.

Conclusions

The solubility values obtained were dependent on the method used. It is thus important to use methods that replicate the clinical environment for meaningful evaluations.

目的：牙科材料的质量控制测试需要一个标准，以便生成可重复和可比较的数据。目前还没有用于检测牙髓治疗材料的标准。本研究的目的是制定一个新的标准来评估牙髓保存材料的溶解度：方法：对用于牙髓治疗的三种材料进行溶解度测试：方法：使用 ISO 4049:2019 (S1) 和 ISO 6876:2012 (S2) 两项牙科材料国际标准评估了 Biodentine、TheraCal 和 Activa 这三种用于牙髓治疗的材料的溶解度。针对这两项标准，采用了一种改进的方法进行评估。这包括改变所用溶液的体积（S1M、S2M），使用杜氏改良鹰培养基（DMEM）代替水（S1D、S2D），以及定期更换 ISO 4049 方法的溶液（S1P、S1MP）。利用扫描电子显微镜（SEM）和 X 射线衍射（XRD）分析法对溶解度测试前后的材料进行了表征：结果：根据所用方法的不同，测试材料表现出不同的溶解度值。在使用两种 ISO 方法进行测试时，如果使用的溶液量较少，则生物丹碱的溶解度明显较低（p ≤ 0.05）。使用 ISO 4049:2019 进行测试时，TheraCal 和 Activa 在干燥后的溶解度值为负值。Biodentine 的微观结构发生了变化，这与测试溶解度的方法有关：所获得的溶解度值取决于所使用的方法。因此，使用能复制临床环境的方法进行有意义的评估非常重要。

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引用次数: 0

Characterization of dental light-curing resin composites incorporating multiple modified low-shrink monomers 含有多种改性低收缩单体的牙科光固化树脂复合材料的特性。

IF 4.6 1区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Dental Materials

Pub Date : 2024-06-12 DOI: 10.1016/j.dental.2024.05.031

Objective

Polymerization shrinkage poses a significant challenge in dental resin composites. The objective of this study is to introduce spiroorthocarbonate monomer 3,9-dimethylene-1,3,5,7-tetraoxa-spiro[5,5]undecane (BMSOC) and epoxy resin monomer 3,4-epoxycyclohexylmethyl-3,4-epoxycyclohexane carboxylate (ECHM-ECHC) into bisphenol-S-bis(3-methacrylato-2-hydroxy propyl)ether (BisS-GMA) based resin composites to develop composites with reduced shrinkage properties.

Methods

BMSOC and BisS-GMA were synthesized and thoroughly mixed with ECHM-ECHC, followed by inorganic fillers and photoinitiators. Based on the composition of the resin matrix, five groups of experimental composites were prepared, with traditional bisphenol A-dimethacrylate glycidyl ester (Bis-GMA)/triethylene glycol dimethacrylate (TEGDMA) based composite serving as the control. The polymerization properties, including degree of conversion (DC) and polymerization shrinkage (PS), as well as marginal microleakage, wettability, flexural strength (FS), flexural modulus (FM), and biocompatibility were evaluated.

Results

The results demonstrated that compared with the control group, the PS of BisS-GMA based composites containing BMSOC and ECHM-ECHC were significantly reduced (P < 0.05), and the lowest PS (0.96 ± 0.08 %) was observed when the ratio of BisS-GMA: (Epoxy + BMSOC) was 4:6. Additionally, the experimental composites also exhibited improved DC, minimal microleakage, low hydrophilicity, enhanced mechanical properties, qualified in vivo biocompatibility, and slight/moderate in vitro biocompatibility.

Significance

The resin composites incorporating multiple modified low-shrink monomers are promising for dental applications to prevent various clinical problems caused by PS and extend restoration longevity.

目的：聚合收缩是牙科树脂复合材料面临的一项重大挑战。本研究的目的是将螺碳酸酯单体 3,9-二亚甲基-1,3,5,7-四氧杂螺[5,5]十一烷（BMSOC）和环氧树脂单体 3,4-环氧环己基甲基-3、4-epoxycyclohexane carboxylate (ECHM-ECHC)加入双酚-S-双（3-甲基丙烯酰氧基-2-羟基丙基）醚（BisS-GMA）基树脂复合材料中，以开发收缩性能更低的复合材料。方法：合成 BMSOC 和 BisS-GMA，并将其与 ECHM-ECHC 充分混合，然后加入无机填料和光引发剂。根据树脂基体的组成，制备了五组实验复合材料，以传统的双酚 A-二甲基丙烯酸缩水甘油酯（Bis-GMA）/三乙二醇二甲基丙烯酸酯（TEGDMA）为基础的复合材料作为对照。评估了聚合性能，包括转化率（DC）和聚合收缩率（PS），以及边缘微渗漏、润湿性、弯曲强度（FS）、弯曲模量（FM）和生物相容性：结果表明，与对照组相比，含有 BMSOC 和 ECHM-ECHC 的基于 BisS-GMA 的复合材料的 PS 显著降低（P<0.05）：含有多种改性低收缩单体的树脂复合材料具有良好的牙科应用前景，可预防 PS 引起的各种临床问题，延长修复体的使用寿命。

{"title":"Characterization of dental light-curing resin composites incorporating multiple modified low-shrink monomers","authors":"","doi":"10.1016/j.dental.2024.05.031","DOIUrl":"10.1016/j.dental.2024.05.031","url":null,"abstract":"<div><h3>Objective</h3><p>Polymerization shrinkage poses a significant challenge in dental resin composites. The objective of this study is to introduce spiroorthocarbonate monomer 3,9-dimethylene-1,3,5,7-tetraoxa-spiro[5,5]undecane (BMSOC) and epoxy resin monomer 3,4-epoxycyclohexylmethyl-3,4-epoxycyclohexane carboxylate (ECHM-ECHC) into bisphenol-S-bis(3-methacrylato-2-hydroxy propyl)ether (BisS-GMA) based resin composites to develop composites with reduced shrinkage properties.</p></div><div><h3>Methods</h3><p>BMSOC and BisS-GMA were synthesized and thoroughly mixed with ECHM-ECHC, followed by inorganic fillers and photoinitiators. Based on the composition of the resin matrix, five groups of experimental composites were prepared, with traditional bisphenol A-dimethacrylate glycidyl ester (Bis-GMA)/triethylene glycol dimethacrylate (TEGDMA) based composite serving as the control. The polymerization properties, including degree of conversion (DC) and polymerization shrinkage (PS), as well as marginal microleakage, wettability, flexural strength (FS), flexural modulus (FM), and biocompatibility were evaluated.</p></div><div><h3>Results</h3><p>The results demonstrated that compared with the control group, the PS of BisS-GMA based composites containing BMSOC and ECHM-ECHC were significantly reduced (<em>P</em> < 0.05), and the lowest PS (0.96 ± 0.08 %) was observed when the ratio of BisS-GMA: (Epoxy + BMSOC) was 4:6. Additionally, the experimental composites also exhibited improved DC, minimal microleakage, low hydrophilicity, enhanced mechanical properties, qualified in vivo biocompatibility, and slight/moderate in vitro biocompatibility.</p></div><div><h3>Significance</h3><p>The resin composites incorporating multiple modified low-shrink monomers are promising for dental applications to prevent various clinical problems caused by PS and extend restoration longevity.</p></div>","PeriodicalId":298,"journal":{"name":"Dental Materials","volume":null,"pages":null},"PeriodicalIF":4.6,"publicationDate":"2024-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141316251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

NP-12 peptide functionalized nanoparticles counteract the effect of bacterial lipopolysaccharide on cultured osteoblasts NP-12肽功能化纳米粒子可抵消细菌脂多糖对培养成骨细胞的影响。

IF 4.6 1区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Dental Materials

Pub Date : 2024-06-12 DOI: 10.1016/j.dental.2024.06.017

Objective

To evaluate whether nanoparticles (NPs) functionalized with Tideglusib (TDg, NP-12), and deposited on titanium surfaces, would counteract the effect of bacterial lipopolysaccharide (LPS) on osteoblasts.

Methods

Experimental groups were: (a) Titanium discs (TiD), (b) TiD covered with undoped NPs (Un-NPs) and (c) TiD covered with TDg-doped NPs (TDg-NPs). Human primary osteoblasts were cultured onto these discs, in the presence or absence of bacterial LPS. Cell proliferation was assessed by MTT-assay and differentiation by measuring the alkaline phosphatase activity. Mineral nodule formation was assessed by the alizarin red test. Real-time quantitative polymerase chain reaction was used to study the expression of Runx-2, OSX, ALP, OSC, OPG, RANKL, Col-I, BMP-2, BMP-7, TGF-β1, VEGF, TGF-βR1, TGF-βR2, and TGF-βR3 genes. Osteoblasts morphology was studied by Scanning Electron Microscopy. One-way ANOVA or Kruskal-Wallis and Bonferroni multiple comparisons tests were carried out (p < 0.05).

Results

TDg-NPs enhanced osteoblasts proliferation. Similarly, this group increased ALP production and mineral nodules formation. TDg-NPs on titanium discs resulted in overexpression of the proliferative genes, OSC and OSX, regardless of LPS activity. In the absence of LPS, TDg-NPs up-regulated Runx2, COL-I, ALP, BMP2 and BMP7 genes. OPG/RANKL gene ratios were increased about 2500 and 4,000-fold by TDg-NPs, when LPS was added or not, respectively. In contact with the TDg-NPs osteoblasts demonstrated an elongated spindle-shaped morphology with extracellular matrix production.

Significance

TDg-NPs on titanium discs counteracted the detrimental effect of LPS by preventing the decrease on osteoblasts proliferation and mineralization, and produced an overexpression of proliferative and bone-promoting genes on human primary osteoblasts.

目的评估用 Tideglusib（TDg，NP-12）功能化的纳米颗粒（NPs）沉积在钛表面是否会抵消细菌脂多糖（LPS）对成骨细胞的影响：实验组为(实验组：(a) 钛盘（TiD）；(b) 未掺杂 NPs 的钛盘（Un-NPs）；(c) 掺杂 TDg 的 NPs 的钛盘（TDg-NPs）。在有或没有细菌 LPS 的情况下，将人类原代成骨细胞培养到这些圆片上。细胞增殖通过 MTT 分析法进行评估，分化通过测量碱性磷酸酶活性进行评估。矿物结节的形成通过茜素红试验进行评估。实时定量聚合酶链反应用于研究 Runx-2、OSX、ALP、OSC、OPG、RANKL、Col-I、BMP-2、BMP-7、TGF-β1、VEGF、TGF-βR1、TGF-βR2 和 TGF-βR3 基因的表达。用扫描电子显微镜研究成骨细胞的形态。进行单因素方差分析或 Kruskal-Wallis 和 Bonferroni 多重比较检验（p 结果：TDg-NPs 增强了成骨细胞的形态：TDg-NPs可促进成骨细胞增殖。同样，该组也增加了 ALP 的产生和矿物质结核的形成。无论 LPS 活性如何，钛盘上的 TDg-NPs 都会导致增殖基因 OSC 和 OSX 的过度表达。在没有 LPS 的情况下，TDg-NPs 会上调 Runx2、COL-I、ALP、BMP2 和 BMP7 基因。在添加或不添加 LPS 的情况下，TDg-NPs 使 OPG/RANKL 基因比率分别增加了约 2500 倍和 4000 倍。与 TDg-NPs 接触的成骨细胞表现出细长的纺锤形形态，并产生细胞外基质：钛盘上的 TDg-NPs 可防止成骨细胞增殖和矿化的减少，从而抵消 LPS 的不利影响，并使人类原发性成骨细胞的增殖基因和促骨基因过度表达。

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引用次数: 0