Dental Materials最新文献_第9页

Diversity of short-term DC outcomes in bulk-fill RBCs subjected to a 3 s high-irradiance protocol 接受 3 秒高辐照方案处理的散装红细胞中短期直流电结果的多样性。

IF 4.6 1区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Dental Materials

Pub Date : 2024-11-01 DOI: 10.1016/j.dental.2024.08.008

Hamad Algamaiah , Abdulrahman Alshabib , Muadh Algomaiah , Jiawei Yang , David C. Watts

Objectives

To determine the short-term (5 min) initial effects of a high-irradiance light-curing (LC) protocol on light transmission (LT%), radiant exposure (RE) and degree of conversion (DC%) of different bulk-fill resin-based composites (RBCs).

Materials and methods

Six bulk-fill composites with different viscosities were investigated: OBF (One Bulk Fill, 3 M), EB (Estelite bulkfill,Tokuyama), PFill, PFlow, ECeram and EFlow (PowerFill, Poweflow, Tetric EvoCeram bulkfill, Tetric Evoflow bulkfill, Ivoclar), subjected to different LC protocols: one ultra-high-intensity (3 W/cm² −3 s via PowerCure LCU) and two conventional (1.2 W/cm² −10 s and 20 s via PowerCure and Elipar S10 LCUs). Specimens (n = 5) were polymerized within their molds (ϕ5 mm

\times

4 mm depth) to determine LT% and RE at 4 mm using a MARC-LC spectrometer. For real-time DC% measurements by FTIR, similar molds were utilized. Data were analyzed by one-way ANOVA and Tukey post-hoc tests at 5 % significance.

Results

Regardless of the applied LC protocols, OBF and low-viscosity RBCs (EB, PFlow and EFlow) had the lowest and highest LT%, RE, DC% and RPmax, respectively. RE results of all RBCs were in the same sequence: Elipar-20 s > PCure-10 s > PCure-3 s. DC% of PFill and PFlow displayed no significant difference between the applied LC protocols (p > 0.05). The polymerization kinetic in all materials was well described by an exponential sum function (r² varied between 0.85 and 0.98), showing a faster polymerization with the PCure-3 s protocol.

Significance

The measurement of LT% and DC% at 5 min gave an insight into the developing polymerization process. The initial response of these bulk-fill composite to a high-irradiation protocol varied depending on their composition and viscosity, being faster for low viscosity materials. Nevertheless, even though multiple resin composites are designed to be efficient during photopolymerization, care should be taken when selecting materials/curing protocol.

目的确定高辐照度光固化（LC）方案对不同松散填充树脂基复合材料（RBC）的透光率（LT%）、辐射照射（RE）和转化率（DC%）的短期（5 分钟）初始影响：研究了六种不同粘度的填充复合材料：OBF (One Bulk Fill, 3 M)、EB (Estelite bulkfill, Tokuyama)、PFill、PFlow、ECeram 和 EFlow (PowerFill, Poweflow, Tetric EvoCeram bulkfill, Tetric Evoflow bulkfill, Ivoclar)。2 W/cm2 -10 s 和 20 s，通过 PowerCure 和 Elipar S10 LCU）。试样（n = 5）在模具（ϕ5 mm × 4 mm 深度）内聚合，使用 MARC-LC 光谱仪测定 4 mm 处的 LT% 和 RE。在使用傅立叶变换红外光谱实时测量 DC% 时，也使用了类似的模具。数据分析采用单因素方差分析和 Tukey 后检验，显著性为 5%：无论采用哪种液相色谱方案，OBF和低粘度RBC（EB、PFlow和EFlow）的LT%、RE、DC%和RPmax分别最低和最高。所有 RBC 的 RE 结果顺序相同：Elipar-20 秒 > PCure-10 秒 > PCure-3 秒。PFill 和 PFlow 的 DC% 在所采用的 LC 方案之间没有显著差异（p > 0.05）。所有材料的聚合动力学都能很好地用指数函数（r2 在 0.85 和 0.98 之间变化）来描述，显示 PCure-3 s 方案的聚合速度更快：意义：通过测量 5 分钟时的 LT% 和 DC%，可以了解聚合过程的发展情况。这些块状填充复合材料对高辐照方案的初始反应因其成分和粘度而异，低粘度材料的反应速度更快。尽管如此，尽管多种树脂复合材料在光聚合过程中的设计效率很高，但在选择材料/固化方案时仍需谨慎。

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引用次数: 0

Effect of phosphoric acid etching and blasting with aluminum oxide on the enamel topography and adhesion of resin composite to intact or abraded enamel 磷酸蚀刻和氧化铝喷射对珐琅质形貌以及树脂复合材料与完整或磨损珐琅质粘附性的影响。

IF 4.6 1区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Dental Materials

Pub Date : 2024-11-01 DOI: 10.1016/j.dental.2024.08.003

Tainah Oliveira Rifane , Ronaldo Hirata , Vitaliano Gomes Araújo-Neto , Thiago Ottoboni , Victor Pinheiro Feitosa , Marcelo Giannini

Objectives

To evaluate the effects of the phosphoric acid (PA) etching, self-etching technique (SE) and blasting with Al₂O₃ particles (BL) on the bonding of a dental adhesive to intact (INT) or abraded (ABR) enamel.

Methods

Enamel surfaces were treated as follows: 1- ABR-PA: INT was abraded with SiC paper and etched with PA (20 s) before Clearfil Universal Bond Quick adhesive application; 2- ABR-SE: ABR was SiC and adhesive applied in SE mode; 3- INT-PA: INT was etched with PA and adhesive applied; 4- INT-SE: the adhesive (SE mode) was applied to INT; 5- INT-BL: INT was BL and the adhesive was applied (SE mode), and 6- INT-BA: INT was BL, etched with PA and adhesive applied (SE mode). The enamel surface treated was examined with scanning electron microscopy (SEM) (n = 3) and Al₂O₃ particles were characterized using SEM and EDX. The enamel bond strength was measured by microtensile test (24 h and 1 year) (n = 8) and the morphology of enamel-adhesive interfaces were analyzed by SEM (n = 3). Bond strength data were analyzed by two-way ANOVA and Tukey’s test (α = 0.05).

Results

Al₂O₃ particles had an irregular shape, their length varied (50–20 µm) and the perimeter mean was 38.8 µm. The enamel morphology significantly influenced the enamel bond strength. ABR-PA, INT-BL, and INT-BA provided greater and stable enamel-dentin interaction and bond strength.

Significance

The enamel morphology significantly influenced the enamel bond strength. Using the adhesive in etch-and-rinse mode, enamel must be abraded before etching and must be Al₂O₃-blasted when used in SE mode.

目的评估磷酸（PA）腐蚀、自酸洗技术（SE）和氧化铝颗粒喷射（BL）对牙科粘合剂与完整（INT）或磨损（ABR）珐琅质粘合的影响：珐琅质表面的处理方法如下：1- ABR-PA：1- ABR-PA：在使用 Clearfil 通用粘接快速粘接剂之前，用碳化硅纸研磨 INT 并用 PA 进行蚀刻（20 秒）；2- ABR-SE：ABR 为碳化硅，粘接剂以 SE 模式使用；3- INT-PA：4- INT-SE：在 INT 上涂抹粘合剂（SE 模式）；5- INT-BL：INT 为 BL，涂抹粘合剂（SE 模式）；6- INT-BA：INT 为 BL，涂抹 PA，涂抹粘合剂（SE 模式）。用扫描电子显微镜（SEM）对处理过的珐琅质表面进行检测（n = 3），并用 SEM 和 EDX 对 Al2O3 颗粒进行表征。通过显微拉伸试验（24 小时和 1 年）测量了珐琅质粘接强度（n = 8），并用扫描电镜分析了珐琅质与粘接剂界面的形态（n = 3）。粘接强度数据采用双因素方差分析和 Tukey 检验（α = 0.05）：结果：Al2O3 颗粒形状不规则，长度不一（50-20 µm），平均周长为 38.8 µm。珐琅质形态对珐琅质粘接强度有明显影响。ABR-PA、INT-BL和INT-BA提供了更大更稳定的珐琅质-牙本质相互作用和粘接强度：珐琅质形态对珐琅质粘接强度有重要影响。在蚀刻-冲洗模式下使用该粘合剂时，必须在蚀刻前对珐琅质进行研磨，在 SE 模式下使用时，必须对珐琅质进行 Al2O3 喷砂处理。

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引用次数: 0

Fatigue lifetime of reduced-diameter implants placed in different bone models 在不同骨模型中植入小直径种植体的疲劳寿命。

IF 4.6 1区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Dental Materials

Pub Date : 2024-11-01 DOI: 10.1016/j.dental.2024.09.003

Pedro Henrique Wentz Tretto , Jason Alan Griggs , Kartikeya Singh Jodha , Yuanyuan Duan , Susana María Salazar Marocho , Alvaro Della Bona

Objectives

This study assessed the fatigue lifetime of reduced-diameter implants placed in either bovine rib or polymer-based bone model.

Methods

Bovine ribs were classified according to the criteria proposed by Lekholm and Zarb and were analyzed for bone fraction. Fourteen dental implants (3.25 mm in diameter × 15 mm in length) were placed in bovine ribs used as a bone model. They were subjected to resonance frequency analysis. Stainless steel loading hemisphere caps were bonded on the abutments position at 30-degree angle and with a moment arm of 11 mm. Accelerated life testing using the step-stress method was conducted at 2 Hz with a stress ratio of 0.1 until fracture on a servo-hydraulic load frames machine (MTS). Results were compared with those of a previous study wherein implants were placed in a polymer-based bone model. Fatigue lifetime statistics (characteristic lifetime and Weibull modulus) of physical specimens were estimated in a reliability analysis software (ALTA PRO). Fractured specimens were examined under an electron scanning microscope to determine the failure mode.

Result

The implants exhibited high stability quotient values (75.07 ± 3.81). Implants placed in bovine ribs showed better data dispersion and longer fatigue lifetime than those placed in polymer-based bone models, with no significant difference between groups. All fractures occurred in the implant body near the bone level and were indicative of fatigue fractures.

Significance

Bovine ribs appear to be a more suitable material for accelerated life testing than the polymer-based material because of better data dispersion.

研究目的本研究评估了在牛肋骨或聚合物骨模型中植入小直径种植体的疲劳寿命：方法：根据 Lekholm 和 Zarb 提出的标准对牛肋骨进行分类，并对骨量进行分析。将 14 个牙科植入体（直径 3.25 毫米 × 长度 15 毫米）植入作为骨模型的牛肋骨中。对它们进行共振频率分析。不锈钢加载半球帽以 30 度角粘接在基台上，力矩臂为 11 毫米。在伺服液压载荷框架机（MTS）上使用阶跃应力法以 2 Hz 的频率进行加速寿命测试，应力比为 0.1，直至断裂。测试结果与之前将植入物植入聚合物骨模型的研究结果进行了比较。在可靠性分析软件（ALTA PRO）中估算了物理试样的疲劳寿命统计数据（特征寿命和威布尔模量）。在电子扫描显微镜下对断裂试样进行检查，以确定失效模式：结果：植入物显示出较高的稳定性商值（75.07 ± 3.81）。与植入聚合物骨模型的种植体相比，植入牛肋骨的种植体显示出更好的数据分散性和更长的疲劳寿命，组间无显著差异。所有骨折都发生在靠近骨水平的植入体上，并显示为疲劳骨折：牛肋骨似乎比聚合物材料更适合用于加速寿命测试，因为其数据分散性更好。

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引用次数: 0

Synthesis and characterization of the dental adhesive monomer 10-MDP 牙科粘合剂单体 10-MDP 的合成与表征。

IF 4.6 1区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Dental Materials

Pub Date : 2024-11-01 DOI: 10.1016/j.dental.2024.09.009

Paula Roberta Perondi Furtado, Rafael Minski Savanhago, Nataly Castro, Rogerio Aparecido Gariani, Marcia Margarete Meier

Many studies have demonstrated the excellent performance of 10-MDP (10-methacryloyloxydecyl dihydrogen phosphate) as a functional monomer for dental adhesive materials and as a primer for ceramic surfaces. Although adhesive performance is affected by the purity level of 10-MDP, this parameter is rarely described, and possible byproducts have been suggested in the literature, but have not been identified to date. The present study aims to present an accessible 10-MDP synthesis strategy with easily handled reagents and address the characterization challenges, especially in identifying byproducts. 10-MDP was synthesized from 10-hydroxydecyl methacrylate and phosphorus pentoxide in acetone. The final product was characterized by nuclear magnetic resonance (NMR), infrared spectroscopy (FTIR) and mass spectrometry MALDITOF/TOF. The main chemical groups associated with 10-MDP were identified by ¹H, ³¹P, and ¹³C NMR analyses. Only mass spectrometry analyses (MALDITOF/TOF) could identify the presence of dimers as byproducts. Its proposed chemical structure indicates that the dimers were formed by the reaction between the phosphate ester groups and others formed by the reaction of the methacrylic group of 10-MDP molecules. Careful adjustment of the synthesis conditions to reduce the formation of these byproducts is also described. The results indicate that the characterization of 10-MDP batches as raw materials is an important task because, depending on the byproduct present, its ability to polymerize or acid etching capacity may be compromised.

许多研究已经证明，10-MDP（10-甲基丙烯酰氧癸基磷酸二氢酯）作为牙科粘合材料的功能单体和陶瓷表面的底漆，具有卓越的性能。尽管 10-MDP 的纯度会影响粘合剂的性能，但很少有人对这一参数进行描述，文献中也提出了可能存在的副产品，但迄今为止尚未发现。本研究旨在利用易于处理的试剂提出一种简便的 10-MDP 合成策略，并解决表征方面的难题，尤其是在确定副产品方面。10-MDP 是由甲基丙烯酸 10- 羟基癸酯和五氧化二磷在丙酮中合成的。核磁共振（NMR）、红外光谱（FTIR）和质谱 MALDITOF/TOF 对最终产品进行了表征。通过 1H、31P 和 13C 核磁共振分析，确定了与 10-MDP 相关的主要化学基团。只有质谱分析（MALDITOF/TOF）能确定二聚物作为副产品的存在。其拟议的化学结构表明，二聚体是由磷酸酯基团和其他由 10-MDP 分子的甲基丙烯酸基团反应形成的。研究还描述了为减少这些副产物的形成而对合成条件进行的精心调整。结果表明，对作为原材料的 10-MDP 批次进行鉴定是一项重要任务，因为根据存在的副产品，其聚合能力或酸蚀刻能力可能会受到影响。

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引用次数: 0

Inflammatory and adhesion profile of gingival fibroblasts to lithium disilicate ceramic surfaces 牙龈成纤维细胞对二硅酸锂陶瓷表面的炎症和粘附情况。

IF 4.6 1区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Dental Materials

Pub Date : 2024-11-01 DOI: 10.1016/j.dental.2024.09.014

Jéssica Ferraz Carvalho Lima , Filipe Milazzo Santos , Taís Browne de Miranda , Guilherme Gama Ramos , Denise Carleto Andia , Adriano F. Lima , Danilo Lazzari Ciotti

Objectives

Lithium disilicate (LS) ceramic emerges as a compelling option for customized implant abutments. However, ensuring its safety and reliability requires clarification on key aspects, notably its impact on inflammation and potential for cell adhesion. This study delves into these considerations, examining the influence of LS ceramic on cytokine release and the transcriptional profile of human gingival fibroblasts (hGFs) in direct contact with various LS surfaces.

Methods

hGFs were cultured on LS disks featuring three distinct surfaces (unpolished, polished, and polished glaze), while titanium disks served as reference material and cells cultured directly on plates as controls. The surface of the disks was analyzed using a scanning electron microscope. The cell metabolism was analyzed by MTT test, cytokine release by MAGPIX and the expression of genes related to cell adhesion was evaluated by qPCR.

Results

The disks exhibited similar topography with smooth surfaces, except for the unpolished LS disks, which had an irregular surface. Contact with LS surfaces did not substantially reduce cell metabolism. Moreover, it generally decreased cytokine release compared to controls, particularly pro-inflammatory mediators like IL-1β, IL-6, and TNF-α. Significantly increased expression of genes related to cell adhesion to LS was observed, comparable to titanium, the gold standard material for implant abutments.

Significance: This study unveils that LS ceramic not only fails to trigger pro-inflammatory cytokine release, but also significantly enhances gene expression associated with cell adhesion. These mechanisms are closely linked to gene pathways such as PTK2, SRC, MAPK1, and transcription factors ELK-1 and MYC. In summary, the findings underscore LS ceramic's potential as a biocompatible material for implant abutments, shedding light on its favorable inflammatory response and enhanced cell adhesion properties.

目的：二硅酸锂（LS）陶瓷作为定制种植基台的一种引人注目的选择。然而，要确保其安全性和可靠性，就必须澄清一些关键问题，特别是其对炎症的影响和细胞粘附的可能性。方法：在具有三种不同表面（未抛光、抛光和抛光釉面）的 LS 盘上培养 hGF，钛盘作为参考材料，细胞直接在平板上培养作为对照。使用扫描电子显微镜分析磁盘表面。用 MTT 测试分析了细胞代谢情况，用 MAGPIX 分析了细胞因子的释放情况，用 qPCR 评估了与细胞粘附有关的基因的表达情况：结果：除未抛光的 LS 盘表面不规则外，其他 LS 盘表面光滑，形貌相似。与 LS 表面接触并不会显著降低细胞的新陈代谢。此外，与对照组相比，细胞因子的释放量普遍减少，尤其是IL-1β、IL-6和TNF-α等促炎介质。观察到与细胞粘附在 LS 上相关的基因表达显著增加，与种植基台的金标准材料钛相当：本研究揭示了 LS 陶瓷不仅不会引发促炎细胞因子的释放，而且还能显著增强与细胞粘附相关的基因表达。这些机制与 PTK2、SRC、MAPK1 以及转录因子 ELK-1 和 MYC 等基因通路密切相关。总之，研究结果强调了 LS 陶瓷作为种植基台生物相容性材料的潜力，揭示了其有利的炎症反应和增强的细胞粘附特性。

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引用次数: 0

Stearic acid nanoparticles increase acyclovir absorption by oral epithelial cells 硬脂酸纳米颗粒可增加口腔上皮细胞对阿昔洛韦的吸收。

IF 4.6 1区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Dental Materials

Pub Date : 2024-11-01 DOI: 10.1016/j.dental.2024.07.005

Priti P. Rath , Hardik Makkar , Shruti Vidhawan Agarwalla , Gopu Sriram , Vinicius Rosa

Objective

Acyclovir (ACY) is used to treat oral viral herpes but has low solubility and bioavailability. Stearic acid (SA) is lipophilic and can be combined with drugs. Therefore, this study aimed to characterize the properties of SA nanoparticles in increasing the cellular uptake of ACY by oral epithelial cells. The hypothesis was that SA nanoparticles increase sustained ACY release, are stable, and increase drug uptake.

Methods

The production parameters (duration and amplitude of sonication) were optimized to produce solid lipid nanoparticles (SLN) of SA-containing ACY. Particle stability was characterized under different storage conditions (4 °C and 37 °C for 1, 15, and 45 days). SLN were further characterized for their pharmacokinetic profile, cytotoxicity, in vitro permeability, and ability to modulate gene expression and promote ACY uptake by oral epithelial cells.

Results

Pharmacokinetic studies revealed sustained and diffusional release of ACY from the SLN, with an initial burst release of 15 min. After 45 d of storage, SLN kept at both 4 °C and 37 °C showed a maximum release of > 90 % of the drug at 120 min. Cells treated with SLN presented a significantly higher intracellular drug content than those treated with ACY and significantly increased the genetic expression of TJP-1, OCLN, and ECAD.

Significance

The hypothesis was accepted as SA nanoparticles containing ACY can sustain drug delivery and enhance its absorption into epithelial cells. Therefore, SA nanoparticles are promising for improving ACY uptake in treating oral herpes and other infections caused by HSV-1.

目的：阿昔洛韦（ACY阿昔洛韦（ACY）用于治疗口腔病毒性疱疹，但其溶解度和生物利用度较低。硬脂酸（SA）具有亲脂性，可与药物结合。因此，本研究旨在确定 SA 纳米粒子在增加口腔上皮细胞对 ACY 的吸收方面的特性。假设是 SA 纳米粒子能增加 ACY 的持续释放，具有稳定性，并能增加药物吸收：方法：优化了生产参数（超声持续时间和振幅），以生产含有 ACY 的固体脂质纳米颗粒（SLN）。在不同的储存条件下（4 °C和37 °C，1天、15天和45天）对颗粒的稳定性进行了表征。还进一步研究了纳米脂质颗粒的药代动力学特征、细胞毒性、体外渗透性以及调节基因表达和促进口腔上皮细胞吸收 ACY 的能力：药代动力学研究表明，ACY 可从 SLN 中持续弥散释放，最初的爆发释放时间为 15 分钟。在 4 ℃ 和 37 ℃ 下保存 45 天后，SLN 在 120 分钟时的最大药物释放量大于 90%。用 SLN 处理的细胞的细胞内药物含量明显高于用 ACY 处理的细胞，并且 TJP-1、OCLN 和 ECAD 的基因表达明显增加：假设成立，因为含有 ACY 的 SA 纳米粒子能持续给药并促进上皮细胞对药物的吸收。因此，SA 纳米颗粒有望改善 ACY 的吸收，用于治疗由 HSV-1 引起的口腔疱疹和其他感染。

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引用次数: 0

A bioactive and anti-bacterial nano-sized zirconium phosphate/GO (nZrP/GO) composite: Potential use as a coating for dental implants? 一种生物活性抗菌纳米级磷酸锆/GO（nZrP/GO）复合材料：作为牙科植入物涂层的潜在用途？

IF 4.6 1区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Dental Materials

Pub Date : 2024-11-01 DOI: 10.1016/j.dental.2024.07.007

Ahmed Al-Noaman , Simon Charles Fielding Rawlinson

Objective

Dental implants fabricated from titanium have several limitations and therefore, alternative materials that fulfil the criteria of successful dental implant (bioactivity and anti-bacterial activity) need to be considered. Polyether ether ketone (PEEK) has been suggested to replace titanium implants. However, this material needs surface modification to meet the appropriate criteria. A nano-sized zirconium phosphate/GO (nZrP/GO) composite coating was prepared to improve PEEK’s biological qualities.

Methods

Polished and cleaned PEEK discs were coated with the composite of nZrP doped with 1.25 wt% GO by the soft-template method. To analyze the composite coating, X-ray, atomic force microscopy, and field emission scanning electron microscopy-energy dispersive spectroscopy were used. The adhesion of the coating to PEEK was measured by adhesive tape test. By measuring the optical contact angle, the coated and non-coated samples' differences in wettability were evaluated. Antimicrobial activity was evaluated against S. aureus and E. coli and cytotoxicity tested employing gingival fibroblasts and osteoblast-like cells.

Results

The nZrP/GO composite coating was 23.45 µm thick, was irregular and attached strongly to the PEEK surface. Following coating, the water contact angle dropped to 34° and surface roughness to 13 nm. The coating reduced the count of bacteria two-fold and was non-cytotoxic to mammalian osteoblast-like cells and fibroblasts. A precipitation of nano-calcium-deficient apatite was observed on the surface of the nZrP/GO coating following a 28-day immersion in SBF.

Significance

PEEK-coated with nZr/GO coating is a good candidate as dental implant.

目的：用钛制造的牙科植入体有一些局限性，因此需要考虑使用符合成功牙科植入体标准（生物活性和抗菌活性）的替代材料。有人建议用聚醚醚酮（PEEK）替代钛种植体。然而，这种材料需要进行表面改性才能达到相应的标准。我们制备了纳米级磷酸锆/GO（nZrP/GO）复合涂层，以改善 PEEK 的生物特性：方法：采用软模板法，在抛光和清洁过的 PEEK 盘上涂覆掺有 1.25 wt% GO 的 nZrP 复合涂层。为了分析复合涂层，使用了 X 射线、原子力显微镜和场发射扫描电子显微镜-能量色散光谱仪。涂层与 PEEK 的附着力是通过胶带测试测量的。通过测量光学接触角，评估了涂层和非涂层样品在润湿性方面的差异。对金黄色葡萄球菌和大肠杆菌的抗菌活性进行了评估，并使用牙龈成纤维细胞和类成骨细胞进行了细胞毒性测试：nZrP/GO 复合涂层的厚度为 23.45 µm，呈不规则状，与 PEEK 表面的附着力很强。镀膜后，水接触角降至 34°，表面粗糙度降至 13 nm。涂层使细菌数量减少了两倍，并且对哺乳动物成骨细胞和成纤维细胞无细胞毒性。在 SBF 中浸泡 28 天后，nZrP/GO 涂层表面出现了纳米缺钙磷灰石沉淀：意义：涂有 nZr/GO 涂层的 PEEK 是牙科植入物的理想候选材料。

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引用次数: 0

Guidance on the assessment of biocompatibility of biomaterials: Fundamentals and testing considerations 生物材料生物兼容性评估指南：基本原理和测试注意事项。

IF 4.6 1区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Dental Materials

Pub Date : 2024-11-01 DOI: 10.1016/j.dental.2024.07.020

Vinicius Rosa , Nikolaos Silikas , Baiqing Yu , Nileshkumar Dubey , Gopu Sriram , Spiros Zinelis , Adriano F. Lima , Marco C. Bottino , Joao N. Ferreira , Gottfried Schmalz , David C. Watts

Background

Assessing the biocompatibility of materials is crucial for ensuring the safety and well-being of patients by preventing undesirable, toxic, immune, or allergic reactions, and ensuring that materials remain functional over time without triggering adverse reactions. To ensure a comprehensive assessment, planning tests that carefully consider the intended application and potential exposure scenarios for selecting relevant assays, cell types, and testing parameters is essential. Moreover, characterizing the composition and properties of biomaterials allows for a more accurate understanding of test outcomes and the identification of factors contributing to cytotoxicity. Precise reporting of methodology and results facilitates research reproducibility and understanding of the findings by the scientific community, regulatory agencies, healthcare providers, and the general public.

Aims

This article aims to provide an overview of the key concepts associated with evaluating the biocompatibility of biomaterials while also offering practical guidance on cellular principles, testing methodologies, and biological assays that can support in the planning, execution, and reporting of biocompatibility testing.

背景：评估材料的生物相容性对于确保患者的安全和福祉至关重要，因为它可以防止不良、毒性、免疫或过敏反应，并确保材料长期保持功能而不会引发不良反应。为确保进行全面评估，在规划测试时必须仔细考虑预期应用和潜在暴露情况，以选择相关检测方法、细胞类型和测试参数。此外，对生物材料的成分和特性进行表征可以更准确地了解测试结果，并确定导致细胞毒性的因素。目的：本文旨在概述与评估生物材料生物相容性相关的关键概念，同时就细胞原理、测试方法和生物检测提供实用指导，为生物相容性测试的规划、执行和报告提供支持。

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引用次数: 0

An integrated preventive and therapeutic magnetic nanoparticle loaded with rhamnolipid and vancomycin for combating subgingival biofilms 含有鼠李糖脂和万古霉素的综合预防和治疗磁性纳米粒子，用于对抗龈下生物膜。

IF 4.6 1区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Dental Materials

Pub Date : 2024-11-01 DOI: 10.1016/j.dental.2024.08.005

Wenyan Kang , Ting Zou , Ye Liang , Huaxiang Lei , Rui Zhang , Jun Kang , Zhenquan Sun , Xuechen Li , Shaohua Ge , Chengfei Zhang

Objectives

Mechanical debridement supplemented with antibacterial agents effectively eradicates subgingival biofilms formed in the periodontal pockets of severe periodontitis patients. However, the available antimicrobial agents have limited penetrating ability to kill the bacteria encased in the deep layers of biofilms. This study aimed to fabricate a novel magnetic nanoparticle (MNP) loaded with rhamnolipid (RL) and vancomycin (Vanc, Vanc/RL-Ag@Fe₃O₄) to combat subgingival biofilms.

Methods

The multispecies subgingival biofilm was formed by periodontal pathogens, including Streptococcus oralis (S. oralis), Streptococcus sanguinis (S. sanguinis), Actinomyces naeslundii (A. naeslundii), Porphyromonas gingivalis (P. gingivalis) and Fusobacterium nucleatum (F. nucleatum). Scanning electron microscope (SEM), confocal laser scanning microscopy (CLSM), and quantitative real-time polymerase chain reaction (qRT-PCR) were used to determine the anti-biofilm efficacy of Vanc/RL-Ag@Fe₃O₄ with or without a magnetic field on multispecies subgingival biofilms.

Results

The minimal inhibitory concentration (MIC) values of Vanc/RL-Ag@Fe₃O₄ on S. oralis, S. sanguinis, A. naeslundii, P. gingivalis, and F. nucleatum were 25, 50, 100, 50, and 25 μg/mL, respectively. Vanc/RL-Ag@Fe₃O₄ (200 μg/mL) reduced the 7-d biofilm thickness from 22 to 13 µm by degrading extracellular polymeric substance (EPS) and killing most bacteria except for tolerant F. nucleatum. A magnetic field enhanced the anti-biofilm effect of Vanc/RL-Ag@Fe₃O₄ by facilitating its penetration into the bottom layers of biofilms and killing tolerant F. nucleatum.

Significance

Vanc/RL-Ag@Fe₃O₄ MNPs can release RL, Vanc, and Ag and eradicate subgingival biofilms by disrupting EPS and killing bacteria. Vanc/RL-Ag@Fe₃O₄ combined with a magnetic force is a promising approach for combating periodontal infection.

目的：机械清创辅以抗菌剂可有效清除严重牙周炎患者牙周袋内形成的龈下生物膜。然而，现有抗菌剂的穿透能力有限，无法杀死包裹在生物膜深层的细菌。本研究旨在制造一种负载鼠李糖脂（RL）和万古霉素（Vanc，Vanc/RL-Ag@Fe3O4）的新型磁性纳米粒子（MNP），以对抗龈下生物膜：多菌种龈下生物膜由牙周致病菌形成，包括口腔链球菌（S. oralis）、血链球菌（S. sanguinis）、呐丝隆放线菌（A. naeslundii）、牙龈卟啉单胞菌（P. gingivalis）和核酸镰刀菌（F. nucleatum）。采用扫描电子显微镜（SEM）、激光共聚焦扫描显微镜（CLSM）和实时定量聚合酶链式反应（qRT-PCR）来测定有磁场或无磁场的 Vanc/RL-Ag@Fe3O4 对多菌种龈下生物膜的抗生物膜功效：结果：Vanc/RL-Ag@Fe3O4对S. oralis、S. sanguinis、A. naeslundii、P. gingivalis和F. nucleatum的最小抑菌浓度（MIC）分别为25、50、100、50和25 μg/mL。Vanc/RL-Ag@Fe3O4（200 μg/mL）通过降解细胞外聚合物质（EPS）和杀死大多数细菌（耐药的核酸噬菌体除外），将 7 天的生物膜厚度从 22 微米减少到 13 微米。磁场增强了 Vanc/RL-Ag@Fe3O4 的抗生物膜效果，因为磁场促进了 Vanc/RL-Ag@Fe3O4 向生物膜底层的渗透，并杀死了耐受的 F. nucleatum：Vanc/RL-Ag@Fe3O4 MNPs 可释放 RL、Vanc 和 Ag，并通过破坏 EPS 和杀死细菌来根除龈下生物膜。Vanc/RL-Ag@Fe3O4与磁力相结合，是一种很有前景的防治牙周感染的方法。

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引用次数: 0

Sol-gel-derived calcium silicate cement incorporating collagen and mesoporous bioglass nanoparticles for dental pulp therapy 用于牙髓治疗的溶胶凝胶硅酸钙水泥，含有胶原蛋白和介孔生物玻璃纳米颗粒。

IF 4.6 1区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Dental Materials

Pub Date : 2024-11-01 DOI: 10.1016/j.dental.2024.08.006

Hazel O. Simila , Caroline Anselmi , Lais M. Cardoso , Renan Dal-Fabbro , Ana M. Beltrán , Marco C. Bottino , Aldo R. Boccaccini

Objective

Calcium silicate cements (CSCs) are often used in endodontics despite some limitations related to their physical properties and antibacterial efficacy. This study aimed to develop and demonstrate the viability of a series of CSCs that were produced by sol-gel method and further modified with mesoporous bioactive glass nanoparticles (MBGNs) and collagen, for endodontic therapy.

Methods

Calcium silicate (CS) particles and MBGNs were synthesized by the sol-gel method, and their elemental, molecular, and physical microstructure was characterized. Three CSCs were developed by mixing the CS with distilled water (CS+H₂O), 10 mg/mL collagen solution (CS+colH₂O), and MBGNs (10 %) (CSmbgn+colH₂O). The mixing (MT) and setting (ST) times of the CSCs were determined, while the setting reaction was monitored in real-time. Antibacterial efficacy against Enterococcus faecalis (E. faecalis) and regenerative potential on dental pulp stem cells (DPSCs) were also analyzed.

Results

The CS+H₂O displayed a ST comparable to commercial products, while CSmbgn+colH₂O achieved the longest MT of 68 s and the shortest ST of 8 min. All the experimental CSCs inhibited the growth of E. faecalis. Additionally, compared to the control group, CSCs supported cell proliferation and spreading and mineralized matrix production, regardless of their composition.

Significance

Tested CSCs presented potential as candidates for pulp therapy procedures. Future research should investigate the pulp regeneration mechanisms alongside rigorous antibacterial evaluations, preferably with multi-organism biofilms, executed over extended periods.

目的：硅酸钙水门汀（CSCs）尽管在物理性质和抗菌效果方面存在一些局限性，但仍经常用于牙髓治疗。本研究旨在开发并证明一系列通过溶胶-凝胶法生产并用介孔生物活性玻璃纳米颗粒（MBGNs）和胶原蛋白进一步修饰的 CSCs 在牙髓治疗中的可行性：方法：采用溶胶-凝胶法合成了硅酸钙（CS）颗粒和 MBGNs，并对其元素、分子和物理微观结构进行了表征。通过将 CS 与蒸馏水（CS+H2O）、10 mg/mL 胶原蛋白溶液（CS+colH2O）和 MBGNs（10 %）（CSmbgn+colH2O）混合，制备了三种 CSC。测定了 CSCs 的混合时间（MT）和凝固时间（ST），并对凝固反应进行了实时监测。此外，还分析了对粪肠球菌（E. faecalis）的抗菌效果以及牙髓干细胞（DPSCs）的再生潜力：结果：CS+H2O 的 ST 值与商业产品相当，而 CSmbgn+colH2O 的 MT 值最长为 68 秒，ST 值最短为 8 分钟。所有实验性 CSCs 都抑制了粪大肠杆菌的生长。此外，与对照组相比，无论其成分如何，造血干细胞都支持细胞增殖、扩散和矿化基质的生成：意义：经过测试的造血干细胞具有作为牙髓治疗程序候选细胞的潜力。未来的研究应在研究牙髓再生机制的同时进行严格的抗菌评估，最好是对多生物体生物膜进行长期评估。

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引用次数: 0