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Clinical researches of a specialized sports nutrition product “Hemosport” for the regulation of hemoglobin levels in adolescent girls who are professionally involved in sports 专业运动营养品“hemsport”调节青春期专业运动女性血红蛋白水平的临床研究
Pub Date : 2022-04-30 DOI: 10.47529/2223-2524.2022.1.4
E. S. Tokaev, E. A. Nekrasov, I. Krasnova, A. A. Khasanov
The aim of the research was a clinical assessment of the effectiveness of the developed specialized sports nutrition product for regulating the level of hemoglobin in adolescent girls professionally involved in sports.Materials and methods: 23 girls — athletes of various sports specialization were researched during a training camp. The athletes were determined by the clinical parameters of blood, its general analysis and biochemical parameters, then the physical endurance and performance were assessed.Results: the developed product “Hemosport” demonstrated efficiency in most of the researched parameters in the main group compared with the control. The positive dynamics of most of the researched indicators revealed a general tendency to improve the state of vital organs and systems of the body of athletes of the main group, to increase their adaptive capabilities and vegetative balance.Conclusions: clinical studies of the specialized product “Hemosport” allow it to be recommended in the practice of sports nutrition for adolescent girls of 12–17 years old who are professionally involved in sports to compensate for iron deficiency nutritional deficiencies specific for this group of athletes, as well as to increase the efficiency and effectiveness of their training.
该研究的目的是临床评估开发的专业运动营养产品对调节从事专业运动的青春期女孩血红蛋白水平的有效性。材料与方法:对23名不同运动专业的女运动员进行了研究。通过血液临床指标、一般分析指标和生化指标测定运动员的体能耐力和运动成绩。结果:与对照组相比,开发的产品“hemsport”在主要组的大多数研究参数中都显示出效率。大多数研究指标的积极动态揭示了改善主体群体运动员身体重要器官和系统状态,提高其适应能力和营养平衡的总体趋势。结论:专业产品“hemsport”的临床研究允许在运动营养实践中推荐12-17岁专业从事运动的青春期女孩,以补偿这组运动员特有的缺铁营养缺乏症,并提高其训练的效率和效果。
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引用次数: 0
Efficiency of isotonic drinks for rehydration of athletes of children and youth age 等渗饮料对儿童和青少年运动员补水的功效
Pub Date : 2022-04-30 DOI: 10.47529/2223-2524.2022.1.6
R. Khanferyan, I. Radysh, V. N. Vybornov, Z. G. Ordzhonikidze, V. I. Pavlov, M. Korosteleva, A. A. Antonov
TThe most important factor limiting sports performance is insufficient fluid intake by athletes and dehydration of the body, while a significant decrease in performance and sports endurance begins with a dehydration level of 2 % and above.Objective: to study the effectiveness and safety of rehydration of the body of athletes­skiers of children and youth using isotonic drinks «Izonok» and «Izonok+».Materials and methods: the main group (n = 12, 16.2 ± 1.4 years) took isotonic drinks for 30 days to compensate for fluid losses, the comparison group (n = 8, 15.7 ± 1.1 years) — drinking water. Bioimpedance body composition analysis was carried out on a KM-AR-01-”Diamant” device (RF, St.Petersburg), and central hemodynamic analysis was carried out on a «SIMONA 111» (LLC “Okulyus 2000”, RF). The content of secretory immunoglobulin A (sIgA) and lysozyme in saliva samples of athletes was carried out by the enzyme immunoassay on an ANTHOS 2010 analyzer (Austria). Statistical data processing was carried out using the Statistica 6.0 software package from Stat Soft @ Ink USA.Results: The intake of an isotonic drink is accompanied by the stimulation of the production of secretory immunoglobulin (sIgA) and lysozyme compared to the consumption of drinking water. The total volume of fluid and the volume of extracellular fluid tended to increase; the volume of intracellular fluid remained practically unchanged; the amount of total water when taking isotonic increased.Conclusion: The investigated isotonic drinks “Izonok” and “Izonok+” are effective sports drinks for maintaining optimal water-salt balance and local immunity
限制运动表现的最重要因素是运动员的液体摄入不足和身体脱水,而表现和运动耐力的显著下降始于脱水水平2%及以上。目的:研究儿童和青少年滑雪运动员使用等渗饮料“伊佐诺”和“伊佐诺+”补液的有效性和安全性。材料与方法:试验组(n = 12, 16.2±1.4年)连续30天服用等渗饮料弥补体液损失,对照组(n = 8, 15.7±1.1年)连续30天饮水。生物阻抗体成分分析在KM-AR-01-“Diamant”装置(RF,圣彼得堡)上进行,中央血流动力学分析在«SIMONA 111»(LLC“Okulyus 2000”,RF)上进行。采用酶免疫分析法,在奥地利ANTHOS 2010型分析仪上测定运动员唾液样品中分泌性免疫球蛋白A (sIgA)和溶菌酶的含量。统计数据处理采用美国Stat Soft @ Ink公司的Statistica 6.0软件包。结果:与喝水相比,等渗饮料的摄入会刺激分泌免疫球蛋白(sIgA)和溶菌酶的产生。体液总量和细胞外液体积呈增加趋势;细胞内液的体积几乎保持不变;等渗时总水量增加。结论:所研究的等渗饮料“伊佐诺”和“伊佐诺+”是维持最佳水盐平衡和局部免疫的有效运动饮料
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引用次数: 0
The effectiveness of physical activity in cardiorehabilitation 体育活动在心脏康复中的有效性
Pub Date : 2022-04-30 DOI: 10.47529/2223-2524.2022.1.1
M. Yakovlev, O. D. Lebedeva, V. Vladimirsky, E. V. Vladimirsky, A. N. Lunina
The review shows that the molecular mechanisms initiated by physical exertion underlie the multifactorial influence of the latter on the function of the cardiovascular system and the course of cardiac diseases. Exercise is an important component of the therapeutic treatment in patients with cardiovascular diseases, which is confirmed by the results of a meta­analysis that included 63 studies that were associated with various forms of aerobic exercise of different intensity (from 50 to 95 % VO2) for 1 to 47 months, which showed that exercise-based CR improves cardiovascular function. Knowledge of the molecular basis of the impact of physical activity makes it possible to use biochemical markers to assess the effectiveness of rehabilitation programs.
综上所述,体力消耗引发的分子机制是体力消耗对心血管系统功能和心脏病病程多因素影响的基础。运动是心血管疾病患者治疗的重要组成部分,一项荟萃分析的结果证实了这一点,该荟萃分析包括63项研究,这些研究与1至47个月的不同强度的各种形式的有氧运动(从50%到95% VO2)有关,表明基于运动的CR可以改善心血管功能。对身体活动影响的分子基础的了解使得使用生化标记物来评估康复计划的有效性成为可能。
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引用次数: 0
Autonomous balance is a factor of changes in the components of the body composition of football players 自主平衡是足球运动员身体组成成分变化的一个因素
Pub Date : 2022-04-30 DOI: 10.47529/2223-2524.2022.1.8
D. Golubev
Objective: To investigate the nature of the relationship between the indicators of heart rate variability (HRV), reflecting the state of autonomous balance and the parameters of the component composition of the body of football players.Materials and methods: We studied n = 80 football players living in the north­western region of the Russian Federation. Group data: 16.9 ± 0.8 years; weight 73.1 ± 4.7 kg; height 181.1 ± 4.9 cm. Monitoring of heart rate variability was carried out by the hardware and software complex “Omega-Sport, St.Petersburg”. The bioelectric activity of the heart was recorded in the II standard lead from the sitting position in the morning (9:00–10:00). The assessment of the body composition of football players was carried out four times (January, April, August, November) using the Inbody 770 analyzer, Japan.Results: SI has a strong direct correlation with the indicators: “total fat content” (r = 0.994, P = 0.011); “total water content” (r = 0.811, P = 0.013). A direct strong correlation was established between SDNN and “total fat content” (r = 0.933, P = 0.009) and an inverse strong correlation with “total muscle mass” (r = -0.877, P = 0.006). pNN50 has a strong direct correlation with “total fat content” (r = 0.889, P = 0.011), in addition, there is a strong inverse correlation with “total muscle mass” (r = ­0.767, P = 0.012). HF and “total fat content” (r = 0.733, P = 0.008) have a strong direct correlation; and with the indicator “total water content” of skeletal muscle mass (r = -0.874, P = 0.011) a strong inverse. A strong inverse relationship is shown between LF and “total fat content” (r = –0.766, P = 0.011), as well as a strong direct correlation with “total muscle mass” (r = 0.951, P = 0.013), “total water content” (r = 0.917, P = 0.008).Conclusions: There is a close correlation of the indicators “total muscle mass”, “total fat content”, “total water content” with a limited number of HRV parameters: SI, SDNN, pNN50, HF, LF. The study showed a significant (p < 0.05) predominance of: the activity of the parasympathetic ANS department — the competitive period, in which there was a significant (p < 0.05) increase in “total muscle mass”, a decrease in “total fat content” in the 1st, 2nd and 3rd diagnostics; the activity of the sympathetic ANS department — the recovery period, which significantly (p < 0.05) affected: a decrease in “total muscle mass”, “total water content”; an increase in “total fat content” in the 4th diagnosis.
目的:探讨反映自主平衡状态的心率变异性指标(HRV)与足球运动员身体各成分组成参数之间的关系性质。材料和方法:我们研究了居住在俄罗斯联邦西北部地区的80名足球运动员。组数据:16.9±0.8岁;重量73.1±4.7 kg;高度181.1±4.9 cm。心率变异性的监测由硬件和软件综合体“Omega-Sport, St.Petersburg”进行。上午(9:00-10:00)坐位用II标准导联记录心脏生物电活动。采用日本Inbody 770分析仪对足球运动员的身体成分进行了4次评估(1月、4月、8月、11月)。结果:SI与“总脂肪含量”有较强的直接相关性(r = 0.994, P = 0.011);总含水量(r = 0.811, P = 0.013)。SDNN与“总脂肪含量”呈正相关(r = 0.933, P = 0.009),与“总肌肉质量”呈负相关(r = -0.877, P = 0.006)。pNN50与“总脂肪含量”呈强正相关(r = 0.889, P = 0.011),与“总肌肉质量”呈强负相关(r = -0.767, P = 0.012)。HF与“总脂肪含量”有较强的直接相关性(r = 0.733, P = 0.008);与骨骼肌质量“总含水量”指标呈强负相关(r = -0.874, P = 0.011)。LF与“总脂肪含量”呈强负相关(r = -0.766, P = 0.011),与“总肌肉质量”(r = 0.951, P = 0.013)、“总含水量”(r = 0.917, P = 0.008)呈强直接相关。结论:“总肌肉质量”、“总脂肪含量”、“总含水量”指标与有限的HRV参数SI、SDNN、pNN50、HF、LF密切相关。该研究显示:副交感神经ANS部门的活动显著(p < 0.05)优势-竞争期,在第一,第二和第三诊断中“总肌肉质量”显著(p < 0.05)增加,“总脂肪含量”显著(p < 0.05)减少;交感ANS部的活动-恢复期,显著(p < 0.05)影响:“总肌肉量”,“总含水量”的减少;第四种诊断中“总脂肪含量”增加。
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引用次数: 0
Some aspects of the influence of extreme climatic factors on the physical performance of athletes 极端气候因素对运动员体能表现影响的几个方面
Pub Date : 2022-04-30 DOI: 10.47529/2223-2524.2022.1.5
I. Kobelkova, M. Korosteleva, D. Nikityuk
Professional athletes often have to participate in competitions in climatic conditions that differ from the optimal or habitual ones for their place of residence. In this regard, it seems relevant to the question of how borderline and extreme external conditions (low and high ambient temperatures, changes in atmospheric pressure, altitude) affect sports performance and endurance. The review presents the biochemical mechanisms underlying the adaptation of athletes to environmental conditions. The human body maintains a fairly constant internal temperature (in some articles — the core) of the body at a level of 37 ± 10C throughout its life, despite a wide range of environmental parameters. The intensity of the processes providing for the release of heat is reflexively regulated. The neurons responsible for heat exchange are located in the center of thermoregulation of the hypothalamus. In the course of evolution, mammals have developed a variety of mechanisms for regulating body temperature, including nervous and humoral, that affect energy metabolism and behavioral responses. There are two ways of heat generation: contractile thermogenesis, due to contractions of skeletal muscles (a special case — cold muscle tremors), and non-contractile — when the processes of cellular metabolism are activated: lipolysis (in particular, brown adipose tissue) and glycolysis. When exposed to extreme ambient temperatures, the thermoregulatory system adjusts to maintain a stable core body temperature by preventing heat loss and increasing heat production in cold conditions, or increasing heat dissipation if the ambient temperature rises. The ambient temperature corresponding to 20–25 ºС on land and 30–35 ºС in water is considered thermoneutral for humans in a state of relative rest. However, any deviations from these conditions, especially against the background of intense physical exercise, can lead to functional overstrain, decreased endurance and sports performance.
职业运动员经常不得不在不同于其居住地的最佳或习惯气候条件下参加比赛。在这方面,它似乎与边缘和极端的外部条件(低和高的环境温度,大气压力的变化,海拔)如何影响运动表现和耐力的问题有关。本文综述了运动员适应环境条件的生化机制。尽管环境参数范围很广,但人体在其一生中保持相当恒定的体内温度(在某些文章中-核心)在37±10摄氏度的水平。提供热量释放的过程的强度是反射性调节的。负责热交换的神经元位于下丘脑的体温调节中心。在进化过程中,哺乳动物已经发展出多种调节体温的机制,包括神经和体液机制,这些机制影响能量代谢和行为反应。热的产生有两种方式:收缩产热,由于骨骼肌的收缩(一种特殊情况-冷肌震颤),和非收缩产热-当细胞代谢过程被激活时:脂肪分解(特别是棕色脂肪组织)和糖酵解。当暴露在极端环境温度下时,体温调节系统通过在寒冷条件下防止热量损失和增加产热或在环境温度升高时增加散热来保持稳定的核心体温。对于处于相对休息状态的人类来说,陆地上20-25ºС和水中30-35ºС的环境温度被认为是热中性的。然而,任何偏离这些条件的情况,特别是在激烈的体育锻炼的背景下,都可能导致功能性过度紧张,耐力和运动表现下降。
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引用次数: 0
Acute mitral chodae rupture in the early postcovid in heavy physical active men. Case series 重度体力活动男性冠状病毒后早期急性二尖瓣破裂案例系列
Pub Date : 2022-04-30 DOI: 10.47529/2223-2524.2022.1.2
Z. Sukmarova, Y. Ovchinnikov, O. Larina, S. O. Lependin, O. V. Afonina, A. Gromov
The opinion that COVID-19 is a greater threat only to the elderly people has changed over the past year. Experience has been accumulated in the development of complications of varying severity in young patients who had optimal health indicators before infection. The consequences of myocarditis are most dangerous, especially in athletes and military personnel. We present a series of clinical cases of spontaneous mitral valve chordae rupture in highly trained middle-aged men in the early post-COVID period. In all cases, the infection proceeded subclinically; SARS-CoV-2 was verified only by analysis for IgM. 1–2 weeks after infection, against the background of a routine training process, patients felt pain in the heart area, which was underestimated. Patients presented for help at 2 and 10 weeks with complaints of reduced endurance and shortness of breath. Echocardiography revealed rupture of one of the chords of the anterior part of the mitral valve against the background of signs of myocarditis with the development of valvular insufficiency of the 1st degree. By the time of treatment, the pathology of other laboratory data and ECG was not observed. The control after 6 months showed in 1 patient a focus of myocardial fibrosis according to MRI, a minimal increase in NT-proBNP, a decrease in exercise tolerance, in 2 patients there was no visible fibrosis, normal NT-proBNP and complete restoration of exercise tolerance, but a decrease in local myocardial deformation according to echocardiography.
过去一年,“新冠肺炎只对老年人构成更大威胁”的观点发生了变化。在感染前健康指标最佳的年轻患者中,已积累了不同严重程度并发症的发展经验。心肌炎的后果是最危险的,特别是在运动员和军人中。我们报告了一系列在covid后早期训练有素的中年男性自发性二尖瓣索断裂的临床病例。在所有病例中,感染都是亚临床进行的;SARS-CoV-2仅通过IgM分析得到验证。感染后1-2周,在常规训练过程的背景下,患者感到心脏部位疼痛,这被低估了。患者在第2周和第10周出现耐力下降和呼吸短促的症状。超声心动图显示二尖瓣前部的一条脊索破裂,背景是心肌炎的迹象,并发展为1度瓣膜功能不全。治疗时未观察其他实验室病理资料及心电图。对照组6个月后1例MRI显示心肌纤维化病灶,NT-proBNP轻度升高,运动耐量降低,2例未见明显纤维化,NT-proBNP正常,运动耐量完全恢复,超声心动图显示局部心肌变形减少。
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引用次数: 1
Parameters of blood pressure during stress test in young elite athletes 青年优秀运动员压力测试中血压参数的研究
Pub Date : 2022-04-30 DOI: 10.47529/2223-2524.2022.1.10
V. Komoliatova, D. Besportochnii, L. Makarov, I. Kiseleva, N. Aksenova
It is known that in children at rest, BP values depend on height, but this aspect is not taken into account when analyzing the maximum BP during exercise.Objective: to determine the maximum value of BP in young elite athletes, depending on height and gender.Materials and methods: 2313 (age 15.5 ± 1.4 years, 45% of young men) young elite athletes, members of national teams of the Russian Federation in 40 sports, were examined. All subjects underwent bicycle ergometry according to the PWC170 protocol, blood pressure was measured manually at each stage of the load, and its maximum values were determined during the entire test.Results: The maximum BP values in boys were higher than in girls: systolic blood pressure (SBP) 195 ± 25 vs 175 ± 20 mmHg, p < 0.001, diastolic blood pressure (DBP) 80 ± 11 vs 80 ± 10 mm Hg, p < 0.05. A relationship was found between the maximum value of blood pressure on load and growth (r = 0.55; p < 0.001). Normative tables are proposed for assessing the maximum values of SBP and load, depending on growth.Conclusion: In young elite athletes, the maximum BP value during VEM according to the PWC170 protocol depends not only on gender, but also on height. The maximum values of SBP during exercise in tall young elite athletes can reach 250 mm Hg in boys and 210 mm Hg in girls.
众所周知,在儿童休息时,血压值取决于身高,但在分析运动时的最大血压时,没有考虑到这一点。目的:确定青年优秀运动员在身高和性别上的血压最大值。材料与方法:对俄罗斯联邦国家队40个项目的优秀青年运动员2313人(年龄15.5±1.4岁,占青年男性的45%)进行调查。所有受试者根据PWC170方案进行自行车几何测量,在负荷的每个阶段手动测量血压,并在整个测试过程中确定其最大值。结果:男孩的最大血压值高于女孩:收缩压(SBP) 195±25 vs 175±20 mmHg, p < 0.001;舒张压(DBP) 80±11 vs 80±10 mmHg, p < 0.05。负荷血压最大值与生长之间存在相关性(r = 0.55;P < 0.001)。根据生长情况,提出了用于评估SBP和负荷最大值的规范表。结论:在年轻优秀运动员中,根据PWC170方案,VEM期间的最大BP值不仅与性别有关,还与身高有关。年轻高个优秀运动员运动时收缩压最大值男孩可达250 mm Hg,女孩可达210 mm Hg。
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引用次数: 0
Clinical evaluation of effectiveness of new product of specialized sports nutrition for correction of psychophysiological state and neuromuscular transmission in highly qualified athletes 专业运动营养新产品对高水平运动员心理生理状态及神经肌肉传导矫正效果的临床评价
Pub Date : 2022-04-19 DOI: 10.47529/2223-2524.2021.4.4
E. S. Tokaev, T. Pushkina, E. A. Nekrasov, I. Krasnova, A. A. Khasanov
The purpose of the study is to determine the effectiveness of the specialized product Fit Tonus for correcting the psychophysiological state and neuromuscular transmission in highly qualified athletes.Materials and methods: the objects of research were: specialized product of sports nutrition for correcting the psychophysiological state and neuromuscular transmission Fit Tonus; 21 male martial arts athletes aged 14 to 25 years. The researches were carried out in accordance with official and conventional techniques. Body temperature, breathing rate, blood pressure level, heart rate was determined, heart rate variability, ECG, external breathing, O2 intake, total urine test, clinical blood test, biochemical blood indices were evaluated, water sectors of the body, body skinny weight, body fat weight were determined. Full-function cardiopulmonary analysis was performed using the Meta Lyzer 3B inpatient cardio-respiratory loading diagnostic system (Cortex Medical, Germany). Maximum oxygen consumption was determined by a Cosomed metabolograph (Italy). Biochemical parameters (bilirubin, ACT, ALT, urea, uric acid, glucose, cholesterol, HGT, amylase, alkaline phosphatase, creatinine) were examined on a Humalyzer biochemical analyzer. Water sectors of the body, the value of skinny body weight and fat body weight were determined on an analyzer for assessing the balance of water sectors of the body (Medass, Russia).Results: in the analysis of biochemical blood values, the blood levels of indicator enzymes and their isoforms were normalized in a short time, despite high training loads. This fact reflects the adaptation of the athlete’s body to high-intensity physical activity when taking the Fit Tone product. The obtained results of the increase in GUS, FFV and Tiffno test indicate an increase in both functional capabilities and functional abilities of the external breathing system, as well as an increase in the efficiency of the respiratory center in athletes who received the tested product. The effectiveness of the test product is confirmed by the results of dynamic and stress samples in a stabilometric study. Results of examination of psychophysical status of single wrestlers showed that during usage of developed product such indicators as performance, vegetative coefficient, dynamism, speed of movement, accuracy of actions, stability of actions are significantly increased. At the same time, the level of fatigue and anxiety is reliably reduced.Conclusions: the results of the researches performed showed that the product developed demonstrated efficacy for most of the parameters under research in the main group compared to the control (placebo).
本研究的目的是确定专用产品Fit Tonus在纠正高水平运动员的心理生理状态和神经肌肉传递方面的有效性。材料与方法:研究对象为:矫正心理生理状态和神经肌肉传导的运动营养专业产品;14至25岁的男子武术运动员21名。这些研究是按照官方和传统的技术进行的。测定体温、呼吸频率、血压水平、心率、心率变异性、心电图、外呼吸、摄氧量、尿总量、临床血检、血液生化指标,测定身体各水段、体瘦体重、体脂体重。使用Meta Lyzer 3B住院患者心肺负荷诊断系统(德国皮质医学公司)进行全功能心肺分析。最大耗氧量由Cosomed代谢仪测定(意大利)。在Humalyzer生化分析仪上检测生化指标(胆红素、ACT、ALT、尿素、尿酸、葡萄糖、胆固醇、HGT、淀粉酶、碱性磷酸酶、肌酐)。在用于评估身体水部门平衡的分析仪上测定身体水部门、瘦体重和胖体重的值(Medass,俄罗斯)。结果:在血生化值分析中,尽管训练负荷很大,但指示酶及其同工型的血水平在短时间内恢复正常。这一事实反映了运动员在服用Fit Tone产品时身体对高强度体力活动的适应。GUS、FFV和Tiffno测试的结果表明,使用测试产品的运动员体外呼吸系统的功能能力和功能能力都有所提高,呼吸中枢的效率也有所提高。在稳定性研究中,动态和应力样品的结果证实了测试产品的有效性。单手摔跤运动员的心理生理状态检测结果表明,在使用该产品的过程中,运动员的竞技性能、营养系数、动力、动作速度、动作准确性、动作稳定性等指标均有显著提高。与此同时,疲劳和焦虑的程度也得到了可靠的降低。结论:所进行的研究结果表明,与对照组(安慰剂)相比,所开发的产品在主要组中对研究中的大多数参数都有疗效。
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引用次数: 0
Preanalytical features of the determination of circulating microRNAs as new specific biomarkers of the body’s response to physical activity 循环microrna作为身体对体力活动反应的新的特异性生物标志物的分析前特征
Pub Date : 2021-12-25 DOI: 10.47529/2223-2524.2021.4.1
P. Postnikov, I. Pronina
MicroRNAs are small non-coding single-stranded RNAs, 18 to 25 nucleotides long, they regulate gene expression at the post-transcriptional level through specific binding to the target mRNA, leading to its degradation. In recent decades, the development of technologies for determining the expression profiles of miRNAs has become an important part of research projects, and the role of miRNAs as potential highly informative molecular biomarkers of various physiological and pathological processes in the body is actively explored by the scientific community. In particular, physical activity is an important modifying factor for circulating miRNAs. Unlike classical blood biochemical parameters, which can change over time depending on the temperature and storage conditions of the sample, microRNAs remain stable during storage and even after multiple freeze-thaw cycles, which makes them an attractive and easily accessible target for detection. However, the determination of the microRNA expression profile in clinical practice is still difficult due to the high heterogeneity of analytical procedures used for testing. In sports medicine, the preanalytical stage is especially important, since often the conditions for sampling are not standardized and can affect the analysis result. This review shows the role of miRNAs as new sensitive biomarkers of the effectiveness of the training process and regulators of the body’s response to physical activity, and also discusses some preanalytical aspects of the analysis of miRNA expression profiles.
MicroRNAs是一种小的非编码单链rna,长度为18 - 25个核苷酸,它们通过特异性结合靶mRNA,在转录后水平调控基因表达,导致其降解。近几十年来,确定miRNAs表达谱的技术发展已成为研究项目的重要组成部分,miRNAs作为机体各种生理和病理过程的潜在高信息分子生物标志物的作用正在被科学界积极探索。特别是,身体活动是循环mirna的重要修饰因素。与经典的血液生化参数不同,这些参数会随着时间的推移而变化,这取决于样品的温度和储存条件,而microrna在储存过程中甚至在多次冻融循环后保持稳定,这使得它们成为一个有吸引力且易于获取的检测目标。然而,由于用于测试的分析程序的高度异质性,在临床实践中确定microRNA表达谱仍然很困难。在运动医学中,分析前阶段尤其重要,因为采样条件往往不规范,可能会影响分析结果。这篇综述显示了miRNA作为训练过程有效性的新的敏感生物标志物和身体对体育活动反应的调节剂的作用,并讨论了miRNA表达谱分析的一些分析前方面。
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引用次数: 0
Modern (rational) methods for detecting genetic features of athletes 检测运动员遗传特征的现代(合理)方法
Pub Date : 2021-12-25 DOI: 10.47529/2223-2524.2021.4.2
A. Zholinsky, A. I. Kadykova, V. Feshchenko, M. Hovhannisyan, A. V. Zorenko, R. Deev
Molecular genetic methods are an integral part of recent medicine. Polymerase chain reaction, Sanger sequencing, next-generation sequencing are widely used in many areas: diagnostics of infectious, inherited, oncological diseases, prenatal screening, study of polymorphisms that increase the risk of developing multifactorial diseases or promoting development physical qualities necessary to achieve success in sports and competitive activity. The growing demand for genotyping raises a number of ethical and social issues affecting the degree of usefulness of genotyping a healthy person and the scientific reliability of the data obtained using direct-to-consumer genetic testing.The review presents and systematizes the laboratory diagnostic methods used today to study nucleic acids that carry important information about human health and physical qualities.
分子遗传学方法是现代医学的一个组成部分。聚合酶链反应、桑格测序、下一代测序被广泛应用于许多领域:传染病、遗传、肿瘤疾病的诊断、产前筛查、增加多因素疾病风险的多态性研究或促进在体育和竞技活动中取得成功所必需的身体素质的发展。对基因分型的日益增长的需求提出了一些伦理和社会问题,影响到对健康人进行基因分型的有用程度以及直接面向消费者的基因检测所获得的数据的科学可靠性。这篇综述介绍并系统化了目前用于研究携带有关人类健康和身体素质重要信息的核酸的实验室诊断方法。
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引用次数: 0
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Sports medicine: research and practice
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