Environmental Pollution最新文献_第10页

Uncovering soil amendment-induced genomic and functional divergence in soybean rhizosphere microbiomes during cadmium-contaminated soil remediation: Novel insights from field multi-omics

IF 7.6 2区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES

Environmental Pollution

Pub Date : 2025-02-03 DOI: 10.1016/j.envpol.2025.125787

Zhongyi Cheng , Yan He , Nanxi Wang , Laosheng Wu , Jianming Xu , Jiachun Shi

Soil amendments exhibit great potential in reducing cadmium (Cd) bioavailability and its accumulation in crop grains, but their practical implications on microbial characteristics (genomic traits and ecological functions) remain unclear. The objective of this study was to combine metagenomics and metatranscriptomics to track the dynamics of bacterial and viral communities in the soybean rhizosphere during the remediation of Cd-contaminated soil using a commercial Mg-Ca-Si conditioner (CMC), applied at low and high (975 kg ha⁻¹ and 1950 kg ha⁻¹) rates under field conditions. Application of CMC increased the average size and decreased the guanine-cytosine (GC) content of microbial genomes, which were strongly shaped by soil pH and available Cd (ACd). Gene and transcript abundances analysis indicated that CMC promoted the enrichment of Alphaproteobacterial metagenome-assembled genomes (MAGs) carrying czcC gene encoding Cd efflux and dsbB gene encoding disulfide bond oxidoreductase. These genes are closely related to Cd resistance and exhibited notable (p < 0.05) increased expression in CMC-treated soils. Additionally, low and high CMC addition significantly increased viral alpha diversity by 5.7% and 9.6%, and viral activity by 3.3% and 7.8%, respectively, in comparison to the control. Temperate viruses were predicted as the major group (64%) and actively linked to the dominant host, and CMC amendment increased host metabolism and adaptability by enhancing (p < 0.05) the abundance and transcriptional activity of virus-encoded auxiliary metabolic genes (AMGs) involved in heavy metal resistance (ABC transport), sulfur cycling (cysH), and host metabolism (galE and queD) through “piggyback-the-winner” strategy. Structural equation modeling further revealed that CMC application influences Cd accumulation in soybean grains through its direct and indirect effects on soil properties and rhizosphere microbiomes, and highlighted the potential role of rhizosphere viruses in agricultural soil remediation.

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引用次数: 0

The prevalence of antibiotic-resistant fecal bacteria in recreational aquatic environments: Phenotypic and molecular approach

IF 7.6 2区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES

Environmental Pollution

Pub Date : 2025-02-03 DOI: 10.1016/j.envpol.2025.125793

Łukasz Kubera , Damian Rolbiecki , Monika Harnisz , Ewa Kotlarska , Piotr Perliński

The rising incidence of antibiotic resistance poses a significant threat to public health. In recent years the widespread use of antibiotics has led to an increase in the concentration of antibiotic-resistant bacteria also in natural environments. The study was conducted in bathing areas three recreational lakes located in the Zaborski Landscape Park in northern Poland. Water samples were collected in three parallel repetitions in April, June and September 2022. Our study indicates that anthropopressure connected with tourism and recreation promotes the growth of fecal bacteria, including antibiotic-resistant strains, whose significant accumulation was recorded in September, the month marking the end of summer vacation. Antibiotic resistance profiles showed that isolated strains of fecal bacteria were resistant to beta-lactam antibiotics. The highest percentage of Escherichia coli strains showed resistance to cefepime (39.1%), and enterococci to imipenem (26.9%). The amplification of resistance genes confirmed the presence of only selected bla genes in the examined strains of fecal bacteria. The bla_TEM gene was found in 14 strains of Enterococcus faecium (82.4%), in all 4 isolates of Enterococcus faecalis, and in 4 out of 5 unspecified strains of fecal streptococci. In Escherichia coli only bla_CTX gene was identified in one strain. The presence of bla_TEM genes was strongly correlated with the concentration of fecal bacteria, it can therefore be assumed that the presence of resistance genes was caused by direct contamination of the studied lakes with feces containing antibiotic-resistant bacteria, presumably without contamination from other sources. Resistance genes found in the control strains from sewage treatment plants were not identified in the studied isolates. Antibiotic resistance genetic markers found in strains isolated from wastewater may prove helpful in determining the sources of contamination of natural aquatic ecosystems with antibiotic-resistant fecal bacteria and thus ensure efficient management of projects aimed at making these waterbodies available for public use.

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引用次数: 0

Molecular composition of secondary brown carbon from styrene at low-to-high oxidation degrees

IF 7.6 2区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES

Environmental Pollution

Pub Date : 2025-02-03 DOI: 10.1016/j.envpol.2025.125795

Shan Zhang, Kun Li, Xiaowen Chen, Zhaomin Yang, Lin Du

Brown carbon (BrC) and its light absorption properties, linked to molecular chromophores, influence Earth's radiative balance. However, the chemical complex formation of secondary BrC has obscured the relationship between molecular composition and optical characteristics. In this study, we investigated the secondary organic aerosol (SOA) optical properties and BrC chromophores formed from the oxidation of styrene, a typical compound from various anthropogenic emissions. The photochemical age spanned low to high oxidation degrees by deploying a smog chamber and an oxidation flow reactor (OFR). Light absorption increased with rising NO_x concentrations under low oxidation degrees in the smog chamber experiments, followed by a decline with increasing photochemical ages at high oxidation degrees in the OFR experiments, likely attributed to the transition from functionalization to fragmentation reactions. Employing a high-resolution quadrupole time-of-flight mass spectrometer with a diode array detector, we characterized the BrC chromophores at a molecular level. We show that the dominant BrC chromophores in styrene SOA are nitrophenol compounds (e.g., C₆H₅NO₅, C₆H₅NO_4, and C₆H₅NO₃). These nitrophenol compounds are proposed to primarily originate from the OH oxidation of benzaldehyde, an intermediate product in the oxidation of styrene. Overall, this study presents a comprehensive characterization of the light absorption of styrene SOA under varying oxidation degrees and provides insights into the contribution of styrene-derived BrC and its implications for radiative forcing.

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引用次数: 0

Pulse exposure to microplastics depolarizes the goldfish gill: Interactive effects of DOC and differential degradation 脉冲暴露于微塑料使金鱼鳃去极化：DOC和差异降解的相互作用

IF 7.6 2区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES

Environmental Pollution

Pub Date : 2025-02-01 DOI: 10.1016/j.envpol.2024.125434

Lauren Zink, Carolyn Morris, Chris M. Wood

Microplastics (MPs) are constantly degrading while moving through aquatic systems as a result of mechanical abrasion, thermal fluctuations, UV light, and chemical exposure. As such, fish may experience pulse exposures to differentially degraded plastics. This study addresses how pulse exposures, over the course of minutes, to differentially degraded microplastics alters a key ionoregulatory property of the goldfish gill. We used transepithelial potential (TEP) across the gills, a diffusion potential resulting from the differential permeability of cations versus anions, as a sensitive indicator of potential ionoregulatory effects. Virgin (non-degraded) MPs along with mechanically, UV, and thermally degraded plastics immediately depolarized the gills (less negative TEP), whereas chemically degraded MPs resulted in no change to TEP. To further explore the physicochemical interaction between the surface of the gill and MPs, combinations of MPs and a single source of dissolved organic carbon (DOC) were tested and revealed that the presence of DOC negated the effects of MPs at the gill regardless of whether DOC or MPs were introduced first. This study suggests that while MPs have the ability to cause ionoregulatory effects at the gill, the effects of ambient water quality, specifically the presence of DOC, are of greater influence.

微塑料（MPs）在通过水生系统时，由于机械磨损、热波动、紫外线和化学物质暴露而不断降解。因此，鱼类可能会经历脉冲暴露于不同降解的塑料中。本研究解决了脉冲暴露，在几分钟的过程中，不同降解的微塑料如何改变金鱼鳃的一个关键的离子调节特性。我们使用跨鳃上皮电位（TEP），这是一种由阳离子和阴离子的不同渗透性引起的扩散电位，作为潜在离子调节作用的敏感指标。未降解的MPs与机械、紫外线和热降解的塑料一起立即使鳃去极化（负TEP更少），而化学降解的MPs不会改变TEP。为了进一步探索鳃表面与MPs之间的物理化学相互作用，我们测试了MPs与溶解有机碳（DOC）单一来源的组合，结果表明，无论先引入DOC还是MPs， DOC的存在都会抵消MPs对鳃的影响。这项研究表明，虽然MPs有能力在鳃处引起离子调节作用，但环境水质的影响，特别是DOC的存在，影响更大。

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引用次数: 0

2,4-Bisphenol S triggers physiological changes, oxidative stress and lipidome alterations in Gram-positive Enterococcus faecalis at environmental concentrations 2,4-双酚S在环境浓度下引发革兰氏阳性粪肠球菌的生理变化、氧化应激和脂质组改变

IF 7.6 2区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES

Environmental Pollution

Pub Date : 2025-02-01 DOI: 10.1016/j.envpol.2024.125475

Pengyu Chen, Baihui An, Yuxi Hu, Yuqiang Tao

2,4-bisphenol S (2,4-BPS) was an emerging BPS analogue as color developers, widely found in the environment. Fish toxicities, cytotoxicity and antiestrogenic effects of 2,4-BPS have been documented at mg L⁻¹, while the toxicity of 2,4-BPS at environmental concentrations (from ng L⁻¹ to μg L⁻¹) were scarce. Bacteria are identified as important components of the ecosystem, while little is known regarding the ecotoxicity of 2,4-BPS on bacteria. Enterococcus faecalis, a good indicator of faecal contamination and anthropogenic pollution, was exposed to 0.5–50 nmol L⁻¹ 2,4-BPS. 2,4-BPS resulted in significantly decreased growth but notably increased membrane permeability in E. faecalis compared with the control. Hormetic effects on the expression of genes involved in DNA replication and efflux were observed. Inhibition of biofilm formation and induction of oxidative stress were caused by 0.5, 5 and 50 nmol L⁻¹ 2,4-BPS. Fatty acyls, glycerolipids and glycerophospholipids were differentially regulated by 2,4-BPS. Glycerolipid metabolism and glycine, serine and threonine metabolism were significantly altered by 0.5 nmol L⁻¹ 2,4-BPS, compared with glycerophospholipid metabolism disturbed by 5 and 50 nmol L⁻¹ 2,4-BPS, showing concentration-dependent responses. Trend analysis of differential lipids demonstrated that there were three significant clusters, all of which were enriched in glycerophospholipid metabolism. 2,4-BPS elicited the strongest lipidomic responses at 5 nmol L⁻¹. Our study provides evidence for 2,4-BPS-induced toxicity to E. faecalis at environmental concentrations and contributes to a comprehensive understanding of the interaction between 2,4-BPS and Gram-positive bacteria.

2,4-双酚S （2,4-BPS）是一种新兴的BPS类似物，广泛存在于环境中。2,4- bps在mg - L-1时的鱼类毒性、细胞毒性和抗雌激素作用已被记录，而2,4- bps在环境浓度（从ng - L-1到μg -1）下的毒性很少。细菌被认为是生态系统的重要组成部分，而2,4- bps对细菌的生态毒性知之甚少。粪肠球菌是粪便污染和人为污染的良好指标，暴露于0.5 ~ 50 nmol l - 1,2,4 - bps。与对照组相比，2,4- bps显著降低了粪肠杆菌的生长，但显著增加了粪肠杆菌的膜通透性。对参与DNA复制和外排的基因表达的激效效应进行了观察。0.5、5和50 nmol L-1 2,4- bps均能抑制生物膜的形成和诱导氧化应激。脂肪酰基、甘油脂和甘油磷脂受到2,4- bps的不同调节。与5和50 nmol L-1 2,4- bps干扰甘油磷脂代谢相比，0.5 nmol L-1 2,4- bps显著改变了甘油脂代谢和甘氨酸、丝氨酸和苏氨酸代谢，呈现浓度依赖性反应。差异脂质的趋势分析表明，存在三个显著的聚类，它们都富含甘油磷脂代谢。2,4- bps在5 nmol L-1时引起最强的脂质组学反应。我们的研究为2,4- bps在环境浓度下诱导粪肠杆菌的毒性提供了证据，并有助于全面了解2,4- bps与革兰氏阳性细菌之间的相互作用。

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引用次数: 0

Validation of crAssphage microbial source tracking markers and comparison with Bacteroidales markers for detection and quantification of faecal contaminations in surface water 验证 CrAssphage 微生物源追踪标记并与类杆菌标记进行比较，以检测和量化地表水中的粪便污染物

IF 7.6 2区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES

Environmental Pollution

Pub Date : 2025-02-01 DOI: 10.1016/j.envpol.2024.125403

Shuang Liu , Trillion Surya Lioe , Li Sun , Evelien M. Adriaenssens , Alan J. McCarthy , Raju Sekar

Human-specific faecal contamination has been affecting surface water and is a threat to both the environment and public health due to its potential co-occurrence with pathogens. Extended studies were conducted to detect and quantify faecal contamination using microbial source tracking (MST) markers targeting bacteria and viruses. The prototypical crAssphage, a presumed Bacteroides-infecting phage discovered in 2014, showed superior specificity to human faeces and high abundance in untreated sewage water. This study evaluated the applicability of crAssphage markers, CPQ_056 and CPQ_064, as MST tools for detecting domestic sewage contamination in surface water in China. Validation tests based on domestic sewage and animal faecal samples demonstrated high sensitivity/specificity of 100%/96.7% for CPQ_056 and 100%/100% for CPQ_064 within the scope of this study, surpassing the performance of traditional Bacteroidales markers such as HF183 (100%/80.4% against sewage). MST markers targeting different hosts and validated in the Taihu watershed (CPQ_056, CPQ_064, BacUni, HF183 TaqMan, Pig-2-Bac, and GFD) were quantified in water samples collected from the inflow rivers of Taihu Lake in summer and winter 2020. The results showed the dominance of sewage/wastewater as the source of contamination in all faecal pollution. Spatial analysis revealed higher contamination levels in northwest rivers, which were those most impacted by human activities. There was also a diluting pattern downstream of some rivers. Correlations with water quality parameters indicated the co-occurrence of nutrient-related pollution and faecal contamination, particularly in areas with industrial, low-density residential, green space, and municipal service land uses. The findings established the efficacy of crAssphage markers in enhancing precision and accuracy in monitoring faecal contamination, offering valuable tools for policymakers and environmental managers.

人类特有的粪便污染一直影响着地表水，由于其可能与病原体同时存在，因此对环境和公众健康都构成了威胁。我们利用针对细菌和病毒的微生物源追踪（MST）标记进行了扩展研究，以检测和量化粪便污染。2014年发现的原型crAssphage是一种推测为感染巴氏杆菌的噬菌体，在未经处理的污水中显示出卓越的人类特异性和高丰度。本研究评估了crAssphage标记物CPQ_056和CPQ_064作为微生物源追踪（MST）工具检测中国地表水中生活污水污染的适用性。基于生活污水和动物粪便样本的验证测试表明，在本研究范围内，CPQ_056 和 CPQ_064 的灵敏度/特异度分别为 100%/96.7%和 100%/100%，超过了 HF183 等传统类杆菌标记物（针对污水的灵敏度/特异度分别为 100%/80.4%）。在 2020 年夏季和冬季采集的入湖河流水样中，对针对不同宿主并在太湖流域得到验证的 MST 标记（CPQ_056、CPQ_064、BacUni、HF183 TaqMan、Pig-2-Bac 和 GFD）进行了定量分析。结果表明，污水废水是所有粪便污染的主要污染源。空间分析表明，受人类活动影响最大的西北部河流污染程度较高。一些河流的下游还出现了稀释模式。与水质参数的相关性表明，营养相关污染和粪便污染同时存在，尤其是在工业区、低密度住宅区、绿地和市政服务区。研究结果表明，crAssphage 标记能有效提高粪便污染监测的精确度和准确性，为政策制定者和环境管理者提供了宝贵的工具。

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引用次数: 0

PM exposure during pregnancy affects childhood asthma via placental epigenetic changes: Neuronal differentiation and proliferation and Notch signaling pathways 妊娠期PM暴露通过胎盘表观遗传改变影响儿童哮喘：神经元分化和增殖以及Notch信号通路

IF 7.6 2区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES

Environmental Pollution

Pub Date : 2025-02-01 DOI: 10.1016/j.envpol.2024.125471

Hyo-Bin Kim , Si Hyeon Lee , Dae Yeol Yang , Seung-Hwa Lee , Jeong-Hyun Kim , Hwan-Cheol Kim , Kil Yong Choi , So-Yeon Lee , Song-I Yang , Dong In Suh , Youn Ho Shin , Kyung Won Kim , Kangmo Ahn , Suk-Joo Choi , Ja-Young Kwon , Soo Hyun Kim , Jong Kwan Jun , Mi-Young Lee , Hye-Sung Won , Kwoneel Kim , Soo-Jong Hong

Particulate matter (PM) exposure during pregnancy increases the risk of developing asthma in children. However, the placental mechanisms have yet to be elucidated. This study aims to evaluate the mechanisms associated with PM exposure during pregnancy and asthma susceptibility via placental epigenetic dysregulation. We analyzed data from two independent Korean birth cohorts (COCOA, 684 children; PSKC, 818 children). Physician-diagnosed current asthma and bronchial hyperresponsiveness (BHR) via methacholine challenge tests were evaluated at age seven. We estimated PM exposure with a diameter <10 μm (PM₁₀) during pregnancy using land-use regression models. We performed genome-wide methylation profiling in the placenta of 40 samples in the COCOA study and analyzed the gene expression levels. High PM₁₀ exposure during pregnancy increased the risk of developing current asthma and BHR in the COCOA study (aOR 2.36, 95% CI 1.06–5.22; aOR 2.14, 95% CI 1.40–3.27, respectively) and current asthma in the PSKC (aOR 2.62, 95% CI 1.35–5.09). The genes involved in neuronal differentiation and proliferation and Notch signaling pathways were significantly hypermethylated in children with high PM₁₀-exposed asthma. The methylation and expression levels of eight genes (PAX6, REST, OLIG2, GLI1, ZBTB7A, NOTCH4, NOTCH1, and NOTCH3) in these pathways correlated with clinical parameters. This may effectively predict PM-related asthma through a prediction model using degrees of gene-based or CpG-based methylation (AUC = 0.96 and 0.93, respectively). PM₁₀ exposure during pregnancy impacts asthma development in offspring via placental DNA hypermethylation via neuronal differentiation and proliferation and Notch signaling pathways.

怀孕期间接触颗粒物（PM）会增加儿童患哮喘的风险。然而，胎盘机制尚未阐明。本研究旨在通过胎盘表观遗传失调来评估妊娠期间PM暴露和哮喘易感性的相关机制。我们分析了两个独立的韩国出生队列(COCOA， 684名儿童；PSKC， 818名儿童)。在7岁时通过甲基胆碱激发试验评估医生诊断的当前哮喘和支气管高反应性（BHR）。我们估计PM暴露的直径为<；10 μm （PM10）。在COCOA研究中，我们对40个样本的胎盘进行了全基因组甲基化分析，并分析了基因表达水平。在COCOA研究中，孕期高PM10暴露增加了当前哮喘和BHR的风险(aOR 2.36, 95% CI 1.06-5.22；aOR为2.14,95% CI分别为1.40-3.27)和PSKC患者当前哮喘（aOR为2.62,95% CI为1.35-5.09）。在高pm10暴露的哮喘儿童中，参与神经元分化和增殖以及Notch信号通路的基因显著高甲基化。这些通路中8个基因（PAX6、REST、OLIG2、GLI1、ZBTB7A、NOTCH4、NOTCH1、NOTCH3）的甲基化和表达水平与临床参数相关。这可以通过使用基于基因或基于cpg的甲基化程度的预测模型（AUC分别= 0.96和0.93）有效地预测pm相关哮喘。妊娠期PM10暴露通过神经元分化、增殖和Notch信号通路，通过胎盘DNA超甲基化影响后代哮喘的发展。

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引用次数: 0

N-acyl-homoserine-lactones as a critical factor for biofilm formation during the initial adhesion stage in drinking water distribution systems n -酰基同丝氨酸内酯是饮用水分配系统初始粘附阶段生物膜形成的关键因素

IF 7.6 2区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES

Environmental Pollution

Pub Date : 2025-02-01 DOI: 10.1016/j.envpol.2024.125489

Ruisi Chen , Ruotong Xu , Jiaxin Huang , Xiuneng Zhu , Yulin Tang , Yongji Zhang

The N-acyl-homoserine-lactone (AHLs)-mediated quorum sensing (QS) system is crucial for the coordination of microbial behaviors within communities. However, the levels of AHLs in biofilms in drinking water distribution systems (DWDSs) and their impact on biofilm formation remain poorly understood. Herein, we simulated DWDSs via biofilm reactors to explore the presence and influence of AHLs during the initial stages of biofilm formation on pipe walls. Glass, polypropylene random copolymer (PP-R) and stainless steel (SS) were used as the coupon materials and the three parallel experimental groups were set up and named accordingly. The glass material is considered to form biofilms only minimally and is therefore used as a negative control. By day 30, the concentration of AHLs in biofilm phase in both PP-R group and SS group reached 1200–1800 ng/L. The predominant AHLs were C6-HSL, C8-HSL, and C10-HSL, with a significant positive correlation between AHLs and biofilm biomass. Metagenomic analysis revealed that microbes exhibiting significant differences among the three groups all demonstrated notable correlations with AHLs. Subsequent analysis of QS genes revealed that the genes associated with AHLs biosynthesis and QS receptors were more abundant in the PP-R and SS groups with biofilm formation. Additionally, we analyzed the abundance of genes related to cell motility, transmembrane transport, tricarboxylic acid cycle, and genetic information synthesis. The co-occurrence network indicates that these processes exhibit a strong correlation with QS genes. This study demonstrates the pivotal role of AHLs in microbial communication during the initial stages of biofilm formation in DWDSs and indicates that the regulatory pathways and mechanisms of AHLs may vary under different environmental conditions.

n -酰基-高丝氨酸-内酯（AHLs）介导的群体感应（QS）系统对群落内微生物行为的协调至关重要。然而，饮用水分配系统（DWDSs）生物膜中ahl的水平及其对生物膜形成的影响仍然知之甚少。本文通过生物膜反应器模拟dwds，探讨AHLs在管壁生物膜形成初期的存在及其影响。以玻璃、聚丙烯无规共聚物（PP-R）和不锈钢（SS）为粘结材料，设置三个平行实验组，并分别命名。玻璃材料被认为只能最小限度地形成生物膜，因此用作阴性对照。第30天，PP-R组和SS组生物膜相AHLs浓度均达到1200 ~ 1800 ng/L。ahl以C6-HSL、C8-HSL和C10-HSL为主，ahl与生物膜生物量呈显著正相关。宏基因组分析显示，三组间存在显著差异的微生物均与ahl呈显著相关。随后对QS基因的分析发现，与AHLs生物合成和QS受体相关的基因在形成生物膜的PP-R和SS组中更为丰富。此外，我们还分析了与细胞运动、跨膜运输、三羧酸循环和遗传信息合成相关的基因丰度。共现网络表明，这些过程与QS基因有很强的相关性。本研究证明了AHLs在dwds生物膜形成初期微生物交流中的关键作用，并表明AHLs在不同环境条件下的调控途径和机制可能存在差异。

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引用次数: 0

Plant species dominance over PFAAs in structuring bacterial communities and their functional profiles in treatment wetlands 植物物种对PFAAs在处理湿地细菌群落结构中的优势及其功能特征

IF 7.6 2区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES

Environmental Pollution

Pub Date : 2025-02-01 DOI: 10.1016/j.envpol.2024.125499

Salvatore Raniolo , Nicola Dal Ferro , Alessandro Pellizzaro , Massimo Fant , Alessandra Tondello , Saptarathi Deb , Piergiorgio Stevanato , Maurizio Borin , Andrea Squartini

This study investigates the impact of different plant species (Iris pseudacorus L., Phragmites australis (Cav.) Trin. Ex Steud., Typha latifolia L.) and solutions containing increasing perfluoroalkyl acids (PFAAs) concentrations (11 perfluorocarboxylic acids and three perfluorosulfonic acids, 500, 2500, and 5000 ng L⁻¹, each) on bacterial communities in treatment wetland (TW) mesocosm systems. The aim was to rank the respective importance of plant species and pollutant concentration in shaping the structure of the selected bacterial communities. While microbial community structure was mainly a function of plant species, PFAAs had some effect on the predictable bacterial functional profiles. Among the tested species, Typha demonstrated exceptional versatility in supporting bacterial communities with enhanced nitrogen, sulfur, and organic compound metabolism, outperforming Iris and Phragmites. Different PFAA concentrations did not modify the overall taxonomical community structure, although they significantly altered the relative abundances of single functional groups. The observed variations in taxonomy-predictable functional groups highlighted the impact of PFAAs on specific plant bacteria, with potential implications also for the metabolism of other specific compounds. The study also identified distinct bacterial functional profiles associated with specific plant species, revealing that bacteria linked to Typha exhibited more specialized functions, while those associated with Phragmites qualified more within the generalist category. This suggests that Typha may be particularly suitable for TWs under PFAA-rich wastewaters, due to its ability to support the metabolism of organic compounds. Additionally, results indicated that phylum diversity may be used as a reliable proxy of functional diversity patterns. Overall, this study contributes to cast light on the intricate relationships between plant species, PFAA concentrations, and bacterial communities and their catabolic functions, which provides an exploitable advancement of knowledge for the optimization of treatment wetlands.

本研究探讨了不同植物种类（鸢尾、芦苇）对植物生长的影响。指标。Steud交货。)和含有不断增加的全氟烷基酸（PFAAs）浓度的溶液（11种全氟羧酸和3种全氟磺酸，每种浓度为500、2500和5000 ng L-1）对处理湿地（TW）生态系统中细菌群落的影响。目的是对植物物种和污染物浓度在形成选定细菌群落结构中的重要性进行排序。虽然微生物群落结构主要是植物物种的功能，但PFAAs对可预测的细菌功能谱有一定影响。在被测试的物种中，Typha在支持细菌群落方面表现出了特殊的多功能性，增强了氮、硫和有机化合物的代谢，优于鸢尾和芦苇。不同浓度的PFAA并未改变群落整体结构，但显著改变了单个功能基团的相对丰度。在分类上可预测的功能群中观察到的变化突出了PFAAs对特定植物细菌的影响，并且对其他特定化合物的代谢也有潜在的影响。该研究还确定了与特定植物物种相关的不同细菌功能概况，揭示了与Typha相关的细菌表现出更多的专门功能，而与芦苇相关的细菌则更符合通才类别。这表明，由于其支持有机化合物代谢的能力，Typha可能特别适合在富含pfa的废水中使用TWs。此外，研究结果表明，门多样性可以作为功能多样性模式的可靠代理。总体而言，本研究有助于揭示植物物种、PFAA浓度、细菌群落及其分解代谢功能之间的复杂关系，为优化处理湿地提供可开发的知识。

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引用次数: 0

Reversibility of polystyrene nanoplastics-induced disruption of testosterone biosynthesis in mice: The role of histone modifications 聚苯乙烯纳米塑料诱导小鼠睾酮生物合成破坏的可逆性：组蛋白修饰的作用

IF 7.6 2区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES

Environmental Pollution

Pub Date : 2025-02-01 DOI: 10.1016/j.envpol.2024.125506

Yan-Yang Lu , Rui Yang , Meiyi Cao , Lu Lu , Wanqing Zhu , Weizhen Hua , Meiping Tian , Yan Sun , Qingyu Huang

Nanoplastics (NPs) exposure could disrupt the synthesis of steroid hormones, thereby posing a potential threat to male reproductive health. However, the existing comprehension of the molecular mechanisms participating in this process remains limited, and the reversibility of NPs-triggered male reproductive toxicity is poorly understood. This investigation focused on the impact of histone modification on testosterone production in mice under long-term exposure to environmentally relevant doses of polystyrene nanoplastics (PS-NPs). The results showed 500 nm and 100 nm PS-NPs could accumulate in mouse testis, with a subsequent significant decrease following a period of self-recovery. The testosterone levels significantly increased after exposure to 500 nm and 100 nm PS-NPs, and the protein levels of CYP11A1, CYP17A1, and 17β-HSD were upregulated. Furthermore, PS-NPs exposure decreased the levels of multiple histone modifications (H3K9me1/2, H3K4me2/3, and H3K4/9ac) while increased H3K9me3 in mouse testis. Histone H3K9 methylation is linked with gene inhibition, whereas H3K4 methylation and H3K4/9 acetylation contribute to gene activation. ChIP analysis further confirmed that H3K9me2 was markedly decreased in the promoter regions of Cyp11a1 and Hsd17b. Additionally, H3K9me2 demethylase Jhdm2a was significantly increased. These findings suggested that low-level PS-NPs inhibited H3K9me2 through upregulating Jhdm2a, thereby activating key steroidogenic proteins CYP11A1 and 17β-HSD, ultimately promoting testosterone synthesis in mouse testis. Importantly, the changes in testosterone, steroidogenic proteins and histone modifications were effectively reversed upon the cessation of exposure to 500 nm and 100 nm PS-NPs. Collectively, these discoveries offer fresh perspectives on the epigenetic mechanisms underlying male reproductive endocrine disruption caused by PS-NPs, and contribute to assessing the human health hazards associated with exposure to environmental NPs.

纳米塑料（NPs）暴露可能破坏类固醇激素的合成，从而对男性生殖健康构成潜在威胁。然而，对参与这一过程的分子机制的现有理解仍然有限，并且对nps引发的男性生殖毒性的可逆性知之甚少。本研究的重点是在长期暴露于环境相关剂量聚苯乙烯纳米塑料（PS-NPs）的小鼠中，组蛋白修饰对睾丸激素产生的影响。结果表明，500 nm和100 nm的PS-NPs可在小鼠睾丸中积累，并在一段时间的自我恢复后显著减少。500 nm和100 nm PS-NPs处理后，睾酮水平显著升高，CYP11A1、CYP17A1和17β-HSD蛋白水平上调。此外，PS-NPs暴露降低了小鼠睾丸中多种组蛋白修饰（H3K9me1/2、H3K4me2/3和H3K4/9ac）的水平，同时增加了H3K9me3的水平。组蛋白H3K9甲基化与基因抑制有关，而H3K4甲基化和H3K4/9乙酰化有助于基因激活。ChIP分析进一步证实，H3K9me2在Cyp11a1和Hsd17b的启动子区域显著降低。H3K9me2去甲基化酶Jhdm2a显著升高。这些发现表明，低水平PS-NPs通过上调Jhdm2a抑制H3K9me2，从而激活关键的类固醇生成蛋白CYP11A1和17β-HSD，最终促进小鼠睾丸激素合成。重要的是，在停止暴露于500 nm和100 nm PS-NPs后，睾酮，类固醇蛋白和组蛋白修饰的变化有效逆转。总的来说，这些发现为PS-NPs引起的男性生殖内分泌紊乱的表观遗传机制提供了新的视角，并有助于评估与暴露于环境NPs相关的人类健康危害。

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