Baijiu is a traditional solid-state distilled liquor prepared from grain fermentation, and is known as Chinese national liquor. Perception of astringency and bitterness is often considered as unpleasant in Baijiu and other alcoholic beverages, yet the underlying oral physiology and saliva biochemistry basis for individualized astringency and bitterness perception remains unclear. This study elucidated how individual differences in saliva influence these perceptions. Twelve subjects were carefully selected and grouped based on high or low recognition thresholds to four previously identified important astringent and bitter compounds: ethyl lactate and n-propanol (astringent), isoamyl alcohol and furfural (bitter/astringent). Unstimulated and stimulated saliva samples were collected and analyzed for pH, total protein, and mucin content. Saliva samples and their mixtures with bitter/astringent stimuli were analyzed using rheology, soft texture analyzer tribometer, and Quartz Crystal Microbalance with Dissipation (QCM-D). Results showed that more sensitive subjects exhibited higher protein/mucin content, resulting in higher shear viscosity and better lubrication, as compared to less sensitive subjects. Upon mixing with each single stimulus, saliva samples from less sensitive subjects showed a decrease in viscosity, suggesting a possible protein network disruption; while an increased viscosity was seen in high recognition threshold samples, implying a different interaction mechanism such as protein aggregation. Tribological and QCM-D analyses further revealed distinct group differences in friction reduction in mixed lubrication regime, surface adsorption, and film viscoelasticity. Astringent compounds generally induced higher mass adsorption and larger viscoelastic shifts. These findings demonstrate that salivary biochemistry directly dictates interaction with trigeminal/taste stimuli, explaining inter-individual sensory sensitivity.
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