Plant Stress最新文献_第7页

PsDUF6A from Populus simonii enhances drought tolerance in transgenic Arabidopsis and poplar by increasing ROS scavenging

IF 6.8 Q1 PLANT SCIENCES

Plant Stress

Pub Date : 2024-12-13 DOI: 10.1016/j.stress.2024.100706

Yanfei Yang , Jinna Zhao , Xingrong Ren , Xueqi Bai , Tao Li , Jianbo Li

Domain of unknown function (DUF) proteins play roles in a range of plant biological processes, including growth and development, and adaptation to abiotic stresses. However, their function was largely unknown in woody plants. Populus simonii is a notable native tree species in northern China and is highly tolerance to drought stress. In this study, PsDUF6A was isolated and functionally characterized from P. simonii. This gene was highly expressed in mature leaves and its expression was induced under drought condition. Transgenic Arabidopsis and 84 K poplar lines overexpressing PsDUF6A were constructed to investigate the function of PsDUF6A in drought tolerance. Under drought conditions, the survival rate and relative water content were higher in PsDUF6A-overexpressing Arabidopsis than in wild-type Arabidopsis, whereas the opposite trend was observed for relative electrical conductivity, indicative of increased drought tolerance. Compared with 84 K poplar, transgenic poplar had a higher photosynthetic activity, lower water loss rate, and higher root biomass. Moreover, PsDUF6A-overexpressing increased antioxidant enzyme activities and the reactive oxygen species scavenging. In addition, the yeast one-hybrid assay indicated that PsC2H213, PsC2H214, PsC2H215, PsC2H217, and PsC2H218 can directly bind to PsDUF6A promoter. These results indicated that PsDUF6A enhances drought tolerance by maintaining ROS homeostasis, and its expression might regulate by C2H2-type ZFPs. These findings revealed the positive contributions of PsDUF6A to drought tolerance and provided insights into the underlying regulatory network of P. simonii response to drought stress.

{"title":"PsDUF6A from Populus simonii enhances drought tolerance in transgenic Arabidopsis and poplar by increasing ROS scavenging","authors":"Yanfei Yang , Jinna Zhao , Xingrong Ren , Xueqi Bai , Tao Li , Jianbo Li","doi":"10.1016/j.stress.2024.100706","DOIUrl":"10.1016/j.stress.2024.100706","url":null,"abstract":"<div><div>Domain of unknown function (DUF) proteins play roles in a range of plant biological processes, including growth and development, and adaptation to abiotic stresses. However, their function was largely unknown in woody plants. <em>Populus simonii</em> is a notable native tree species in northern China and is highly tolerance to drought stress. In this study, <em>PsDUF6A</em> was isolated and functionally characterized from <em>P. simonii</em>. This gene was highly expressed in mature leaves and its expression was induced under drought condition. Transgenic <em>Arabidopsis</em> and 84 K poplar lines overexpressing <em>PsDUF6A</em> were constructed to investigate the function of <em>PsDUF6A</em> in drought tolerance. Under drought conditions, the survival rate and relative water content were higher in <em>PsDUF6A</em>-overexpressing <em>Arabidopsis</em> than in wild-type <em>Arabidopsis</em>, whereas the opposite trend was observed for relative electrical conductivity, indicative of increased drought tolerance. Compared with 84 K poplar, transgenic poplar had a higher photosynthetic activity, lower water loss rate, and higher root biomass. Moreover, <em>PsDUF6A-</em>overexpressing increased antioxidant enzyme activities and the reactive oxygen species scavenging. In addition, the yeast one-hybrid assay indicated that PsC2H213, PsC2H214, PsC2H215, PsC2H217, and PsC2H218 can directly bind to <em>PsDUF6A</em> promoter. These results indicated that <em>PsDUF6A</em> enhances drought tolerance by maintaining ROS homeostasis, and its expression might regulate by C2H2-type ZFPs. These findings revealed the positive contributions of <em>PsDUF6A</em> to drought tolerance and provided insights into the underlying regulatory network of <em>P. simonii</em> response to drought stress.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"15 ","pages":"Article 100706"},"PeriodicalIF":6.8,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143098247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mapping QTLs for aluminium and phosphorus tolerances at seedling and reproductive stages in lentil (Lens culinaris Medikus)

IF 6.8 Q1 PLANT SCIENCES

Plant Stress

Pub Date : 2024-12-12 DOI: 10.1016/j.stress.2024.100709

Noren Singh Konjengbam , Dharmendra Singh , Anima Mahato , Vikram Jeet Singh , Jyoti Taunk

Aluminium toxicity and phosphorus deficiency are primary limitations to crop yield under acidic soil conditions. Developing aluminium (Al) and phosphorous (P) stress tolerant cultivars is one of the effective way to increase plant productivity under acidic soils. Hence, the present study was conducted at 6 different acidic environments during 2020–21 and 2021–22 to map quantitative trait loci (QTLs) associated with Al and low P tolerances in 150 F₇ recombinant inbred lines derived from BM-4 (Al-sensitive) x L-4602 (Al-tolerant) genotypes. Sixteen morpho-physiological and yield related traits were studied in response to Al and P stresses under hydroponic and field conditions. Seven QTLs were identified using composite interval mapping on linkage group 1 for six morpho-physiological traits including root re-growth (RRG), fluorescent signals (callose accumulation), aluminium content, phosphorus content, days to flowering and days to maturity under Al stress (E-1), Al with P stresses (E-2) and acidic field condition (E-6). Individual QTLs accounted for logarithm of odd (LOD) values of 2.73 to 6.85 and phenotypic variation between 5.4 % to 19.8 %. The major QTLs with Al tolerance and P efficiency components can be integrated into elite lentil cultivars using molecular breeding. Improved lentil lines for Al tolerance and P efficiency will reduce the input need for lime application and phosphate fertilizers, significantly cutting costs, together with increased productivity in acidic, and P deficient soils, altogether boosting farmers’ and seed industry profitability.

{"title":"Mapping QTLs for aluminium and phosphorus tolerances at seedling and reproductive stages in lentil (Lens culinaris Medikus)","authors":"Noren Singh Konjengbam , Dharmendra Singh , Anima Mahato , Vikram Jeet Singh , Jyoti Taunk","doi":"10.1016/j.stress.2024.100709","DOIUrl":"10.1016/j.stress.2024.100709","url":null,"abstract":"<div><div>Aluminium toxicity and phosphorus deficiency are primary limitations to crop yield under acidic soil conditions. Developing aluminium (Al) and phosphorous (P) stress tolerant cultivars is one of the effective way to increase plant productivity under acidic soils. Hence, the present study was conducted at 6 different acidic environments during 2020–21 and 2021–22 to map quantitative trait loci (QTLs) associated with Al and low P tolerances in 150 F<sub>7</sub> recombinant inbred lines derived from BM-4 (Al-sensitive) x L-4602 (Al-tolerant) genotypes. Sixteen morpho-physiological and yield related traits were studied in response to Al and P stresses under hydroponic and field conditions. Seven QTLs were identified using composite interval mapping on linkage group 1 for six morpho-physiological traits including root re-growth (RRG), fluorescent signals (callose accumulation), aluminium content, phosphorus content, days to flowering and days to maturity under Al stress (E-1), Al with P stresses (E-2) and acidic field condition (E-6). Individual QTLs accounted for logarithm of odd (LOD) values of 2.73 to 6.85 and phenotypic variation between 5.4 % to 19.8 %. The major QTLs with Al tolerance and P efficiency components can be integrated into elite lentil cultivars using molecular breeding. Improved lentil lines for Al tolerance and P efficiency will reduce the input need for lime application and phosphate fertilizers, significantly cutting costs, together with increased productivity in acidic, and P deficient soils, altogether boosting farmers’ and seed industry profitability.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"15 ","pages":"Article 100709"},"PeriodicalIF":6.8,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143098940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nanoencapsulated nitric oxide donor triggers a dose-dependent effect on the responses of maize seedlings to high light stress

IF 6.8 Q1 PLANT SCIENCES

Plant Stress

Pub Date : 2024-12-12 DOI: 10.1016/j.stress.2024.100711

Diego G. Gomes , Bruno T. Sousa , Joana C. Pieretti , Roney H. Pereira , Wagner R. de Souza , Halley C. Oliveira , Amedea B Seabra

Nanoencapsulation of nitric oxide (NO) donors provides sustained release of NO, prolonging its action on plants. Here, we evaluated the action of NO-releasing chitosan nanoparticles containing GSNO (S-nitrosoglutathione) on the protection of maize plants (Zea mays cv. Balu 787) against high light-induced stress. Experiment 1 was used to compare maize plants under different light intensities. Experiment 2 evaluated the protective effect of chitosan nanoparticles containing GSNO (NPNO) in different concentrations (200 or 400 µM). Experiment 3 compared the protective effect of NPNO to non-nanoencapsulated GSNO (NO) and nanoparticles without NO-releasing molecule (NP). In experiments 1 and 2, chlorophyll a fluorescence and gas exchange measurements were performed. In experiment 3, chlorophyll a fluorescence and biochemical analyses were carried out. In experiment 1, increases in dynamic photoinhibition (DP) of 135 % (Day 1), 370 % (Day 2), 206 % (Day 3), and 100 % (Day 5) were observed from sun plants. In experiment 2, NPNO400 showed higher levels of DP on the first (+ 148 %) and second days (+ 171 %), followed by a reduction on the fifth day (- 22 %). For gas exchange parameters, NPNO400 attenuated the reduction in A at noon and significantly increased k, while NPNO200 decreased the k value. The differences in the effects induced by NPNO treatments are dose-dependent. In experiment 3, NPNO was the only treatment that significantly increased NO bioavailability and the activity of antioxidant enzymes (SOD; POD), contributing to mitigating stress caused by excess light on plants. The nanoencapsulation of NO donors protected maize plants against photoinhibition.

{"title":"Nanoencapsulated nitric oxide donor triggers a dose-dependent effect on the responses of maize seedlings to high light stress","authors":"Diego G. Gomes , Bruno T. Sousa , Joana C. Pieretti , Roney H. Pereira , Wagner R. de Souza , Halley C. Oliveira , Amedea B Seabra","doi":"10.1016/j.stress.2024.100711","DOIUrl":"10.1016/j.stress.2024.100711","url":null,"abstract":"<div><div>Nanoencapsulation of nitric oxide (NO) donors provides sustained release of NO, prolonging its action on plants. Here, we evaluated the action of NO-releasing chitosan nanoparticles containing GSNO (S-nitrosoglutathione) on the protection of maize plants (<em>Zea mays</em> cv. Balu 787) against high light-induced stress. Experiment 1 was used to compare maize plants under different light intensities. Experiment 2 evaluated the protective effect of chitosan nanoparticles containing GSNO (NPNO) in different concentrations (200 or 400 µM). Experiment 3 compared the protective effect of NPNO to non-nanoencapsulated GSNO (NO) and nanoparticles without NO-releasing molecule (NP). In experiments 1 and 2, chlorophyll <em>a</em> fluorescence and gas exchange measurements were performed. In experiment 3, chlorophyll <em>a</em> fluorescence and biochemical analyses were carried out. In experiment 1, increases in dynamic photoinhibition (DP) of 135 % (Day 1), 370 % (Day 2), 206 % (Day 3), and 100 % (Day 5) were observed from sun plants. In experiment 2, NPNO400 showed higher levels of DP on the first (+ 148 %) and second days (+ 171 %), followed by a reduction on the fifth day (- 22 %). For gas exchange parameters, NPNO400 attenuated the reduction in <em>A</em> at noon and significantly increased <em>k</em>, while NPNO200 decreased the <em>k</em> value. The differences in the effects induced by NPNO treatments are dose-dependent. In experiment 3, NPNO was the only treatment that significantly increased NO bioavailability and the activity of antioxidant enzymes (SOD; POD), contributing to mitigating stress caused by excess light on plants. The nanoencapsulation of NO donors protected maize plants against photoinhibition.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"15 ","pages":"Article 100711"},"PeriodicalIF":6.8,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143097827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deciphering the genomic regions associated with seedling cold tolerance traits in rice (Oryza sativa L.)

IF 6.8 Q1 PLANT SCIENCES

Plant Stress

Pub Date : 2024-12-12 DOI: 10.1016/j.stress.2024.100707

Gurjeet Singh , Anjan Kumar Pradhan , Subroto Das Jyoti , Chersty L. Harper , Punniakotti Elumalai , Darlene L. Sanchez , Stanley Omar PB. Samonte , Shyamal K. Talukder

Seedling cold tolerance (SCT) in rice is important for planting rice in colder temperatures that occur during early planting and ratooning in some rice-growing regions in the world. Genome-wide association study (GWAS) has the potential to understand the genetic mechanisms of complex traits like SCT. A set of 204 rice accessions were screened for SCT traits in three environments: natural cold conditions (E1; temperature 6.3 °C-23.3 °C), growth chamber cold conditions (E2; 10 °C in 1st and 2nd weeks followed by 17 °C in 3rd and 4th weeks), and normal growth condition in the greenhouse (E3; day and night temperature maintained at 28–30 °C). Data collected on the number of emerged seedlings at six, eight, and twelve days after sowing, and seedling length (SL) was measured at two and four weeks after sowing. GWAS analysis identified nine quantitative trait nucleotides (QTNs) with phenotypic variation ranging from 10.98 to 20.72%. Among them, S06_22947376, S07_27594541, and S07_3833577 showed pleiotropic responses for multiple traits in different experiments. Candidate gene analysis of S06_22947376 identified four putative genes, i.e., Os06g0585950, Os06g0585982, Os06g0586150, and Os06g0587200 around the region to be associated with a protein kinase responsible for increasing the SCT. The results of this study provide valuable information for understanding the genetic control of SCT and the further development of molecular markers that are useful for breeding programs for the development of rice cultivars tolerant to cold stress. At the same time, rice accessions showing potential SCT will be integrated into the breeding program for varietal development.

{"title":"Deciphering the genomic regions associated with seedling cold tolerance traits in rice (Oryza sativa L.)","authors":"Gurjeet Singh , Anjan Kumar Pradhan , Subroto Das Jyoti , Chersty L. Harper , Punniakotti Elumalai , Darlene L. Sanchez , Stanley Omar PB. Samonte , Shyamal K. Talukder","doi":"10.1016/j.stress.2024.100707","DOIUrl":"10.1016/j.stress.2024.100707","url":null,"abstract":"<div><div>Seedling cold tolerance (SCT) in rice is important for planting rice in colder temperatures that occur during early planting and ratooning in some rice-growing regions in the world. Genome-wide association study (GWAS) has the potential to understand the genetic mechanisms of complex traits like SCT. A set of 204 rice accessions were screened for SCT traits in three environments: natural cold conditions (E1; temperature 6.3 °C-23.3 °C), growth chamber cold conditions (E2; 10 °C in 1st and 2nd weeks followed by 17 °C in 3rd and 4th weeks), and normal growth condition in the greenhouse (E3; day and night temperature maintained at 28–30 °C). Data collected on the number of emerged seedlings at six, eight, and twelve days after sowing, and seedling length (SL) was measured at two and four weeks after sowing. GWAS analysis identified nine quantitative trait nucleotides (QTNs) with phenotypic variation ranging from 10.98 to 20.72%. Among them, S06_22947376, S07_27594541, and S07_3833577 showed pleiotropic responses for multiple traits in different experiments. Candidate gene analysis of S06_22947376 identified four putative genes, i.e., <em>Os06g0585950, Os06g0585982, Os06g0586150</em>, and <em>Os06g0587200</em> around the region to be associated with a protein kinase responsible for increasing the SCT. The results of this study provide valuable information for understanding the genetic control of SCT and the further development of molecular markers that are useful for breeding programs for the development of rice cultivars tolerant to cold stress. At the same time, rice accessions showing potential SCT will be integrated into the breeding program for varietal development.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"15 ","pages":"Article 100707"},"PeriodicalIF":6.8,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143098245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combined transcriptome and metabolome analysis provides insight into the ERF073 – Malic acid network in pakchoi under submergence stress

IF 6.8 Q1 PLANT SCIENCES

Plant Stress

Pub Date : 2024-12-12 DOI: 10.1016/j.stress.2024.100708

Dan-dan Xi , Lu Gao , Li-ao Ge , Hong-fang Zhu , Li-ming Miao , Ding-yu Zhang , Chang-wei Zhang , Ying Li , Yan-xiao Dong , Xi-lin Hou , Yu-ying Zhu , Zhao-hui Zhang , Xiao-feng Li

Pakchoi is sensitive to oxygen deficiency caused by submergence; however, the molecular mechanisms underlying its response to these conditions remains unclear. To determine these mechanisms, two pakchoi cultivars, Heiyoudong (HYD) and Suzhouqing (SZQ), were subjected to submergence for 1 h and 5 h, respectively. Following this treatment, the plants were transferred back to the control conditions for recovery. HYD exhibited higher survival rate than SZQ after recovery. Leaves from 1 h stress, 5 h stress, and subsequent 5-h recovery phases were harvested for metabolome and transcriptome analyses. Data analysis revealed that 206 differentially accumulated metabolites (DAMs) were identified between SZQ and HYD after 1 h stress (S1 vs H1) and 330 DAMs were identified between SZQ and HYD after 5 h stress (S5 vs H5) at stress stage. During recovery stage, 124 and 310 DAMs were found in S1 vs H1 and S5 vs H5, respectively. Additionally, 553 commonly differentially expressed genes (DEGs) were found both in S1 vs H1 and S5 vs H5 at the stress stage, while 458 DEGs were commonly found in the two comparison groups at the recovery stage. Among these DEGs, ERF073 was down-regulated, as further confirmed by qRT-PCR. KEGG pathway analysis revealed that DAMs and DEGs were mainly enriched in metabolic pathways. Taken together, these findings indicate that ERF073 may regulate malic acid accumulation in pakchoi, increasing its resistance to submergence.

{"title":"Combined transcriptome and metabolome analysis provides insight into the ERF073 – Malic acid network in pakchoi under submergence stress","authors":"Dan-dan Xi , Lu Gao , Li-ao Ge , Hong-fang Zhu , Li-ming Miao , Ding-yu Zhang , Chang-wei Zhang , Ying Li , Yan-xiao Dong , Xi-lin Hou , Yu-ying Zhu , Zhao-hui Zhang , Xiao-feng Li","doi":"10.1016/j.stress.2024.100708","DOIUrl":"10.1016/j.stress.2024.100708","url":null,"abstract":"<div><div>Pakchoi is sensitive to oxygen deficiency caused by submergence; however, the molecular mechanisms underlying its response to these conditions remains unclear. To determine these mechanisms, two pakchoi cultivars, Heiyoudong (HYD) and Suzhouqing (SZQ), were subjected to submergence for 1 h and 5 h, respectively. Following this treatment, the plants were transferred back to the control conditions for recovery. HYD exhibited higher survival rate than SZQ after recovery. Leaves from 1 h stress, 5 h stress, and subsequent 5-h recovery phases were harvested for metabolome and transcriptome analyses. Data analysis revealed that 206 differentially accumulated metabolites (DAMs) were identified between SZQ and HYD after 1 h stress (S1 vs H1) and 330 DAMs were identified between SZQ and HYD after 5 h stress (S5 vs H5) at stress stage. During recovery stage, 124 and 310 DAMs were found in S1 vs H1 and S5 vs H5, respectively. Additionally, 553 commonly differentially expressed genes (DEGs) were found both in S1 vs H1 and S5 vs H5 at the stress stage, while 458 DEGs were commonly found in the two comparison groups at the recovery stage. Among these DEGs, ERF073 was down-regulated, as further confirmed by qRT-PCR. KEGG pathway analysis revealed that DAMs and DEGs were mainly enriched in metabolic pathways. Taken together, these findings indicate that ERF073 may regulate malic acid accumulation in pakchoi, increasing its resistance to submergence.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"15 ","pages":"Article 100708"},"PeriodicalIF":6.8,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143098941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Physiological response and transcriptomic analysis of red-fleshed apple seedlings to low temperature stress

IF 6.8 Q1 PLANT SCIENCES

Plant Stress

Pub Date : 2024-12-11 DOI: 10.1016/j.stress.2024.100696

LingYu Meng , Rui Zhang , YanPing Wei , Tingting Gu , Jiahao Zhao , Zijing Chen , Zongying Zhang , Nan Wang , Xuesen Chen , Wenjun Liu

Low temperature (LT) stress negatively impacts the yield and quality of key horticultural crops such as apples. While previous research has predominantly focused on white-fleshed apple varieties with limited cold tolerance, the mechanisms underlying low temperature responses in red-fleshed apples remain largely unexplored. In this study, we used red-fleshed apple seedlings that we developed previously to investigate the biochemical responses to varying temperature conditions. Notably, LT stress significantly enhanced the accumulation of anthocyanins, soluble sugars, soluble proteins, and other metabolites in the red-fleshed apple seedlings. Leveraging RNA-seq data, we identified a potential LT stress-responsive transcription factor, which we designated as MdbHLH51. Functional characterization revealed that overexpression of MdbHLH51 in ‘Orin’ calli significantly promoted anthocyanin accumulation and up-regulated the expression of all MdCBFs genes, thereby bolstering tolerance to cold stress. These findings provide valuable insights into the cold stress response mechanisms in red-fleshed apples, offering a theoretical foundation for the genetic breeding of cold-resistant red apple varieties.

{"title":"Physiological response and transcriptomic analysis of red-fleshed apple seedlings to low temperature stress","authors":"LingYu Meng , Rui Zhang , YanPing Wei , Tingting Gu , Jiahao Zhao , Zijing Chen , Zongying Zhang , Nan Wang , Xuesen Chen , Wenjun Liu","doi":"10.1016/j.stress.2024.100696","DOIUrl":"10.1016/j.stress.2024.100696","url":null,"abstract":"<div><div>Low temperature (LT) stress negatively impacts the yield and quality of key horticultural crops such as apples. While previous research has predominantly focused on white-fleshed apple varieties with limited cold tolerance, the mechanisms underlying low temperature responses in red-fleshed apples remain largely unexplored. In this study, we used red-fleshed apple seedlings that we developed previously to investigate the biochemical responses to varying temperature conditions. Notably, LT stress significantly enhanced the accumulation of anthocyanins, soluble sugars, soluble proteins, and other metabolites in the red-fleshed apple seedlings. Leveraging RNA-seq data, we identified a potential LT stress-responsive transcription factor, which we designated as MdbHLH51. Functional characterization revealed that overexpression of <em>MdbHLH51</em> in ‘Orin’ calli significantly promoted anthocyanin accumulation and up-regulated the expression of all <em>MdCBFs</em> genes, thereby bolstering tolerance to cold stress. These findings provide valuable insights into the cold stress response mechanisms in red-fleshed apples, offering a theoretical foundation for the genetic breeding of cold-resistant red apple varieties.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"15 ","pages":"Article 100696"},"PeriodicalIF":6.8,"publicationDate":"2024-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143097826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Two Sphingomonas species modify the productivity and the susceptibility of Pisum sativum to pea aphid, Acyrthosiphon pisum

IF 6.8 Q1 PLANT SCIENCES

Plant Stress

Pub Date : 2024-12-07 DOI: 10.1016/j.stress.2024.100703

Audrey Pecourt , Manuella Catterou , Candice Mazoyon , Hervé Demailly , Vivien Sarazin , Frédéric Dubois , Jérôme Duclercq , Anas Cherqui

Aphids are major pests of field crops, and their control still largely relies on chemical insecticides, which have significant ecological and health drawbacks. Recent studies suggest that plants, such as pea (Pisum sativum) can recruit beneficial bacteria in the rhizosphere potentially influencing their resilience to insect pests. However, the implications of this microbial recruitment in plant-insect interactions remain underexplored. In this study, we investigated how key rhizosphere bacteria of pea, including Rhizobium leguminosarum, S. sediminicola, and S. daechungensis, modulate pea-aphid (Acyrthosiphon pisum) interactions and affect plant productivity. We assessed both the bottom-up effects of individual and combined bacterial inoculations on plant health and aphid performance, and the top-down effects of aphid infestation on soil functionality. Our results demonstrate that inoculation with S. sediminicola and/or S. daechungensis significantly reduced aphid fecundity, while mitigating aphid-induced stress on pea plants, thereby supporting overall plant growth and productivity. Conversely, aphid infestation negatively impacted soil functionality, potentially disrupting beneficial microbial communities. These findings highlight the potential of targeted microbial recruitment as a sustainable approach to enhance plant productivity and resilience against aphid pests.

{"title":"Two Sphingomonas species modify the productivity and the susceptibility of Pisum sativum to pea aphid, Acyrthosiphon pisum","authors":"Audrey Pecourt , Manuella Catterou , Candice Mazoyon , Hervé Demailly , Vivien Sarazin , Frédéric Dubois , Jérôme Duclercq , Anas Cherqui","doi":"10.1016/j.stress.2024.100703","DOIUrl":"10.1016/j.stress.2024.100703","url":null,"abstract":"<div><div>Aphids are major pests of field crops, and their control still largely relies on chemical insecticides, which have significant ecological and health drawbacks. Recent studies suggest that plants, such as pea (<em>Pisum sativum</em>) can recruit beneficial bacteria in the rhizosphere potentially influencing their resilience to insect pests. However, the implications of this microbial recruitment in plant-insect interactions remain underexplored. In this study, we investigated how key rhizosphere bacteria of pea, including <em>Rhizobium leguminosarum, S. sediminicola</em>, and <em>S. daechungensis</em>, modulate pea-aphid (<em>Acyrthosiphon pisum</em>) interactions and affect plant productivity. We assessed both the bottom-up effects of individual and combined bacterial inoculations on plant health and aphid performance, and the top-down effects of aphid infestation on soil functionality. Our results demonstrate that inoculation with <em>S. sediminicola</em> and/or <em>S. daechungensis</em> significantly reduced aphid fecundity, while mitigating aphid-induced stress on pea plants, thereby supporting overall plant growth and productivity. Conversely, aphid infestation negatively impacted soil functionality, potentially disrupting beneficial microbial communities. These findings highlight the potential of targeted microbial recruitment as a sustainable approach to enhance plant productivity and resilience against aphid pests.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"15 ","pages":"Article 100703"},"PeriodicalIF":6.8,"publicationDate":"2024-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143098267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plasticity of OsERF109 mitigates drought stress by modulating the antioxidant defense system and morphophysiological traits in rice

IF 6.8 Q1 PLANT SCIENCES

Plant Stress

Pub Date : 2024-12-06 DOI: 10.1016/j.stress.2024.100701

Hemangini Parmar , Anjana Goel , V. Mohan Murali Achary , Ramesh V. Sonti , Malireddy K. Reddy

Freshwater shortages, exacerbated by climate change and unpredictable rainfall, significantly hinder global food security. AP2/ERF transcription factors regulate genes that help plants survive and adapt to harsh environmental stresses. Although ERF109 is linked to drought tolerance, its exact role remains unknown. To investigate this, we generated mutant alleles of the OsERF109 gene in an indica rice cultivar and studied their response to simulated drought conditions. Our findings show that the Oserf109 mutation exhibits no visible effect on plant growth and development under optimum environmental conditions. However, the Oserf109 null mutant demonstrated improved drought tolerance and survival rates. This improvement is due to reduced transpiration, lower canopy temperature, and better plant water status. The Oserf109 mutant maintains cellular hydration and membrane stability by increasing proline content under drought stress. The Oserf109 mutant also exhibits enhanced water use efficiency and improved gaseous exchange, which boosts photosynthetic efficiency, strengthens antioxidant defenses, and preserves chloroplast integrity, protecting the plant from oxidative damage during drought. Additionally, Oserf109 knockout plants showed increased tolerance to osmotic stress and reduced sensitivity to ABA, resulting in better seedling growth and higher germination rates. Our research reveals that the Oserf109 mutation enhances rice crop resilience and yield under adverse conditions. These findings illustrated the inhibitory regulatory role of OsERF109 in the drought tolerance mechanism, with the mutant form of this allele demonstrating potential implications in developing resilient rice cultivars for drought stress.

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引用次数: 0

Omics-assisted crop improvement under abiotic stress conditions

IF 6.8 Q1 PLANT SCIENCES

Plant Stress

Pub Date : 2024-12-01 DOI: 10.1016/j.stress.2024.100626

Ali Raza , Sunil S. Gangurde , Karansher Singh Sandhu , Yan Lv

Climate change-driven diverse abiotic stresses continue to negatively affect plant growth and development, ultimately altering sustainable agricultural production and food security. Multi-omics approaches have revolutionized how plant biologists explore stress-responsive, adaptation, and tolerance mechanisms and pathways, driven by improvements in scientific practices. Therefore, this special issue was designed to feature the latest advancements in omics studies to understand and improve the stress acclimation and tolerance mechanisms in diverse plant species.

引用次数: 0

Antagonistic manipulation of ER-protein quality control between biotrophic pathogenic fungi and host induced defense 生物营养致病菌与宿主诱导防御之间er蛋白质量调控的拮抗操纵

IF 6.8 Q1 PLANT SCIENCES

Plant Stress

Pub Date : 2024-12-01 DOI: 10.1016/j.stress.2024.100693

Theoni Margaritopoulou , Konstantinos Kotsaridis , Martina Samiotaki , Spyridon Nastos , Marinos Maratos , Ieronymos Zoidakis , Despoina Tsiriva , Stergios Pispas , Emilia Markellou

The interaction of plants with pathogens during infection is a multifaceted process involving various molecules deriving from both partners. A current goal in combating pathogen virulence is to induce plant resistance using environmentally friendly compounds. Here, we show that chitosan-based nanoparticles loaded with the defense hormone salicylic acid, can efficiently activate defense responses and reactive oxygen species (ROS) production and PATHOGENESIS RELATED-1 (PR1) expression in Arabidopsis thaliana leaves, and reduce conidial germination of the biotrophic pathogenic fungus Podosphaera xanthii. Transcriptomic and proteomic analyses identified immune response-related upregulated transcripts and proteins after nanoparticle application, highlighting the Leucine Rich Repeat (LRR)-, Systemic Acquired Resistance (SAR)-, and glutathione-related protein groups. Examination of P. xanthii during infection at control conditions, identified ribosomal, hydrolase-related, putative secreted and effector proteins, while nanoparticle application significantly downregulated their expression. An in-depth investigation of the highly expressed proteins in P. xanthii and Arabidopsis revealed the involvement of components of endoplasmic reticulum protein quality control (ERQC) in the pathogen-host interaction. The RPS27A effector protein was identified in fungal virulence, while endoplasmic reticulum (ER) protein processing- and glycosyltransferase-related proteins were implicated in plant's induced defense response following nanoparticle application. Overall, these findings demonstrate that the ERQC is dynamically manipulated by both the pathogen for efficient virulence and by elicitors for plant induced defense.

植物在感染过程中与病原体的相互作用是一个多方面的过程，涉及来自双方的各种分子。目前对抗病原体毒力的目标是利用环境友好化合物诱导植物抗性。本研究表明，壳聚糖纳米颗粒负载防御激素水杨酸，可以有效激活拟南芥叶片的防御反应和活性氧（ROS）的产生和发病机制相关因子-1 （PR1）的表达，并降低生物营养致病性真菌Podosphaera xanthii的分生孢子萌发。转录组学和蛋白质组学分析确定了纳米颗粒应用后免疫反应相关的上调转录物和蛋白质，突出了亮氨酸富重复序列(LRR)-，系统性获得性耐药(SAR)-和谷胱甘肽相关蛋白组。在对照条件下，在感染期间对黄氏假单胞菌进行检测，鉴定出核糖体蛋白、水解酶相关蛋白、推定分泌蛋白和效应蛋白，而纳米颗粒的应用显著下调了它们的表达。深入研究了拟南芥和苍山拟南芥中高表达的蛋白，揭示了内质网蛋白质量控制（ERQC）成分参与了病原体-宿主相互作用。RPS27A效应蛋白与真菌毒力有关，而内质网（ER）蛋白加工和糖基转移酶相关蛋白与纳米颗粒应用后植物诱导的防御反应有关。总的来说，这些发现表明，ERQC是动态操纵的病原体有效的毒力和激发子诱导植物防御。

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引用次数: 0