Plant Stress最新文献_第5页

Tritordeum, barley landraces and ear photosynthesis are key players in cereal resilience under future extreme drought conditions

IF 6.8 Q1 PLANT SCIENCES

Plant Stress

Pub Date : 2025-02-07 DOI: 10.1016/j.stress.2025.100765

Ander Yoldi-Achalandabaso , Aitor Agirresarobe , Artūrs Katamadze , Giulia Burini , Omar Vergara-Díaz , Mariana Mota , Cristina Oliveira , Usue Pérez-López , Rubén Vicente

Drought is the main factor limiting cereal production in the Mediterranean basin and Climate Change will exacerbate its effects. Among the strategies to mitigate Climate Change impact on cereal production, we highlight the development of drought-resilient crops better adapted to future extreme conditions, either by i) using heritage germplasm (e.g., landraces) or ii) developing novel species (e.g., crop hybrids). Our study aimed to identify key functional traits and stress-tolerant germplasm to contribute to designing drought-resilient crops under future Mediterranean climatic conditions. For that, we conducted an innovative approach combining a late-sowing field trial with two contrasting water regimes to simulate future extreme drought conditions, the use of high-throughput phenotyping devices and an infrared gas analyser to characterise leaf and ear photosynthesis, biochemistry, growth, and stress responses during the reproductive stage, and a novel linear mixed-effects model to integrate these results with final agronomical data. Modern durum wheat and barley, barley landraces and tritordeum varieties were grown and evaluated as individual plants. Our results identified barley landrace SBCC010 and tritordeum Coique as promising resilient germplasm. These genotypes showed a grain set maintenance and a higher allocation of resources to the ears compared to modern varieties, higher leaf and ear greenness, and ear photosynthesis and thermostability during the reproductive stage, particularly under stress conditions. We conclude the necessity of including ear photosynthesis in the breeding programs relying on adaptive germplasm as barley landraces and novel cereal hybrids as tritordeum to design drought-resilient cereals for future extreme Mediterranean environments.

{"title":"Tritordeum, barley landraces and ear photosynthesis are key players in cereal resilience under future extreme drought conditions","authors":"Ander Yoldi-Achalandabaso , Aitor Agirresarobe , Artūrs Katamadze , Giulia Burini , Omar Vergara-Díaz , Mariana Mota , Cristina Oliveira , Usue Pérez-López , Rubén Vicente","doi":"10.1016/j.stress.2025.100765","DOIUrl":"10.1016/j.stress.2025.100765","url":null,"abstract":"<div><div>Drought is the main factor limiting cereal production in the Mediterranean basin and Climate Change will exacerbate its effects. Among the strategies to mitigate Climate Change impact on cereal production, we highlight the development of drought-resilient crops better adapted to future extreme conditions, either by i) using heritage germplasm (e.g., landraces) or ii) developing novel species (e.g., crop hybrids). Our study aimed to identify key functional traits and stress-tolerant germplasm to contribute to designing drought-resilient crops under future Mediterranean climatic conditions. For that, we conducted an innovative approach combining a late-sowing field trial with two contrasting water regimes to simulate future extreme drought conditions, the use of high-throughput phenotyping devices and an infrared gas analyser to characterise leaf and ear photosynthesis, biochemistry, growth, and stress responses during the reproductive stage, and a novel linear mixed-effects model to integrate these results with final agronomical data. Modern durum wheat and barley, barley landraces and tritordeum varieties were grown and evaluated as individual plants. Our results identified barley landrace SBCC010 and tritordeum Coique as promising resilient germplasm. These genotypes showed a grain set maintenance and a higher allocation of resources to the ears compared to modern varieties, higher leaf and ear greenness, and ear photosynthesis and thermostability during the reproductive stage, particularly under stress conditions. We conclude the necessity of including ear photosynthesis in the breeding programs relying on adaptive germplasm as barley landraces and novel cereal hybrids as tritordeum to design drought-resilient cereals for future extreme Mediterranean environments.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"15 ","pages":"Article 100765"},"PeriodicalIF":6.8,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143377251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Uncovering Botrytis cinerea-induced physiological changes in melon plants using multi-sensor imaging approaches

IF 6.8 Q1 PLANT SCIENCES

Plant Stress

Pub Date : 2025-02-07 DOI: 10.1016/j.stress.2025.100769

Matilde Barón, María Trinidad Moreno-Martín, Mónica Pineda

Botrytis cinerea, a necrotrophic fungus, poses a challenge to melon cultivation, causing severe damage leading to reduced crop yields. Understanding the infection process of B. cinerea is crucial for developing effective control strategies against it in agricultural and horticultural environments. Traditional methods for studying metabolic changes in host plants are time-consuming and, if imaging techniques are used, usually involve a single sensor. This research takes advantage of multiple imaging tools - RGB, thermal, chlorophyll fluorescence, blue-green fluorescence and hyperspectral reflectance devices - to capture a complete picture of physiological changes in melon leaves infected by this fungus. By comparing infected areas with adjacent healthy tissues, key metabolic changes are identified, such as decreased photosynthetic activity and increased oxidative stress, which occur even before visible symptoms appear. The images provide a detailed spatio-temporal map of infection progression and host response, revealing critical aspects of this plant-pathogen interaction. These results highlight the value of integrating multiple imaging technologies for early detection and management of fungal infections in crops. The results also suggest potential applications for precision agriculture, offering a more efficient way to monitor plant health and implement targeted interventions.

{"title":"Uncovering Botrytis cinerea-induced physiological changes in melon plants using multi-sensor imaging approaches","authors":"Matilde Barón, María Trinidad Moreno-Martín, Mónica Pineda","doi":"10.1016/j.stress.2025.100769","DOIUrl":"10.1016/j.stress.2025.100769","url":null,"abstract":"<div><div><em>Botrytis cinerea</em>, a necrotrophic fungus, poses a challenge to melon cultivation, causing severe damage leading to reduced crop yields. Understanding the infection process of <em>B. cinerea</em> is crucial for developing effective control strategies against it in agricultural and horticultural environments. Traditional methods for studying metabolic changes in host plants are time-consuming and, if imaging techniques are used, usually involve a single sensor. This research takes advantage of multiple imaging tools - RGB, thermal, chlorophyll fluorescence, blue-green fluorescence and hyperspectral reflectance devices - to capture a complete picture of physiological changes in melon leaves infected by this fungus. By comparing infected areas with adjacent healthy tissues, key metabolic changes are identified, such as decreased photosynthetic activity and increased oxidative stress, which occur even before visible symptoms appear. The images provide a detailed spatio-temporal map of infection progression and host response, revealing critical aspects of this plant-pathogen interaction. These results highlight the value of integrating multiple imaging technologies for early detection and management of fungal infections in crops. The results also suggest potential applications for precision agriculture, offering a more efficient way to monitor plant health and implement targeted interventions.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"15 ","pages":"Article 100769"},"PeriodicalIF":6.8,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143373072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unravelling mechanisms underlying phosphate-induced susceptibility to Bakanae disease in rice

IF 6.8 Q1 PLANT SCIENCES

Plant Stress

Pub Date : 2025-02-07 DOI: 10.1016/j.stress.2025.100766

Héctor Martín-Cardoso , Gerrit Bücker , Iratxe Busturia , Blanca San Segundo

Rice is one of the most important crops in the world and sustains >50 % of the world's population. Rice production is, however, severely threatened by bakanae disease, caused by the fungus Fusarium fujikuroi. Due to low soil phosphorus bioavailability, phosphorus fertilizers are routinely used to optimize rice production, which has led to excessive P accumulation in rice fields. We show that high phosphate fertilization enhances susceptibility to bakanae. Similarly, MIR399 overexpression increases phosphate content and enhances susceptibility to F. fujikuroi infection. In vivo imaging of the infection process using a green fluorescent protein-expressing F. fujikuroi isolate revealed higher fungal colonization in roots of plants grown under high-phosphate supply compared to plants under low-phosphate, which is in agreement with the observed phenotype of bakanae susceptibility in phosphate-accumulating plants. Moreover, a weaker activation of defense-related genes and reduced accumulation of ROS occurs during infection in rice plants grown under high phosphate supply. Histochemical detection of lignin and suberin showed reduced accumulation of lignin and suberin in roots of rice plants grown under high-phosphate fertilization, which was consistent with a weaker induction of lignin biosynthesis genes and suberin-related genes in those plants. Taken together, these results indicate that phosphate accumulation represses host immune responses and promotes susceptibility to bakanae. This information provides a basis to understand mechanisms underlying phosphate-induced susceptibility to pathogen infection in rice, which might be useful to reduce the use of agrochemicals, pesticides and fertilizers, in protecting rice from bakanae.

{"title":"Unravelling mechanisms underlying phosphate-induced susceptibility to Bakanae disease in rice","authors":"Héctor Martín-Cardoso , Gerrit Bücker , Iratxe Busturia , Blanca San Segundo","doi":"10.1016/j.stress.2025.100766","DOIUrl":"10.1016/j.stress.2025.100766","url":null,"abstract":"<div><div>Rice is one of the most important crops in the world and sustains >50 % of the world's population. Rice production is, however, severely threatened by bakanae disease, caused by the fungus <em>Fusarium fujikuroi</em>. Due to low soil phosphorus bioavailability, phosphorus fertilizers are routinely used to optimize rice production, which has led to excessive P accumulation in rice fields. We show that high phosphate fertilization enhances susceptibility to bakanae. Similarly, <em>MIR399</em> overexpression increases phosphate content and enhances susceptibility to <em>F. fujikuroi</em> infection. <em>In vivo</em> imaging of the infection process using a green fluorescent protein-expressing <em>F. fujikuroi</em> isolate revealed higher fungal colonization in roots of plants grown under high-phosphate supply compared to plants under low-phosphate, which is in agreement with the observed phenotype of bakanae susceptibility in phosphate-accumulating plants. Moreover, a weaker activation of defense-related genes and reduced accumulation of ROS occurs during infection in rice plants grown under high phosphate supply. Histochemical detection of lignin and suberin showed reduced accumulation of lignin and suberin in roots of rice plants grown under high-phosphate fertilization, which was consistent with a weaker induction of lignin biosynthesis genes and suberin-related genes in those plants. Taken together, these results indicate that phosphate accumulation represses host immune responses and promotes susceptibility to bakanae. This information provides a basis to understand mechanisms underlying phosphate-induced susceptibility to pathogen infection in rice, which might be useful to reduce the use of agrochemicals, pesticides and fertilizers, in protecting rice from bakanae.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"15 ","pages":"Article 100766"},"PeriodicalIF":6.8,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143387618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergistic effects of glutathione and zinc seed priming in alleviating salt stress on maize seed germination, metabolite levels, seedling vigor, and nutrient acquisition

IF 6.8 Q1 PLANT SCIENCES

Plant Stress

Pub Date : 2025-02-07 DOI: 10.1016/j.stress.2025.100767

Rehan Ahmad Kasana , Muhammad Iqbal , Qasim Ali , Farah Saeed , Muhammad Rizwan , Rashida Perveen , Jean Wan Hong Yong

The comparative effects of reduced glutathione (GSH) and 0.5 % Zn (applied separately or in combination) were assessed on maize in relation to seed metabolite levels, seedling growth, antioxidative defense mechanism, levels of biochemicals and nutrient acquisition under NaCl stress. The level of applied salinity was 120 mM in Hoagland's nutrient solution. Salinity negatively affected seed germination and the emergence of seedlings; associated with altered seed metabolic activities. The high salinity also elevated the levels of malondialdehyde (MDA), increased reactive oxygen species (ROS) levels, altered metabolite levels, reduced uptake of mineral nutrients and increased the uptake of Na⁺ in maize seedlings. Interestingly, the GSH seed priming protocol, when applied alone or with Zn, ameliorated the physiological negativities associated with high salinity upon maize germination, emergence and seedling development. The three millimolar GSH concentration in combination with Zn (0.5 %) improved the germination attributes and emergence of seedlings. The GSH level of 3 mM with Zn was also effective in mitigating the negative impacts of NaCl salinity on seedling growth, associated with better maintenance of physio-biochemical activities, reduced uptake or translocation of Na²⁺, and better maintenance of the increased K⁺/Na⁺ and Ca²⁺/Na⁺. The improvement in maize salt stress tolerance, attributed to 3 mM GSH with 0.5 % Zn as seed treatment, was associated with reduced Na⁺ uptake that decreased its toxicity. Based on this study, it is plausible to use a combination of GSH and Zn as seed priming agentsto enhance the physiological resilience of maize growing in areas with high salinity.

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引用次数: 0

Comparative transcriptomic analysis and identification of candidate genes related to Verticillium wilt resistance in Gossypium barbadense and Gossypium hirsutum

IF 6.8 Q1 PLANT SCIENCES

Plant Stress

Pub Date : 2025-02-05 DOI: 10.1016/j.stress.2025.100759

Jieyin Zhao , Xingui Hu , Yuxiang Wang, Jiaxin Lu, Wenju Gao, Xuening Su, Quanjia Chen, Yanying Qu

The resistance to Verticillium wilt in Gossypium barbadense is generally greater than that in Gossypium hirsutum, and analyzing the differences in the mechanism and regulatory genes involved in Verticillium wilt resistance between G. barbadense and G. hirsutum is particularly important. Here, we report a transcriptomic study for phenotypic evaluation of Verticillium wilt resistance in G. hirsutum (TM-1) and G. barbadense (Hai7124) and a comparison of the transcriptomes at 7 time points after Verticillium dahliae inoculation. Phenotypic evaluation revealed that, compared with TM-1, Hai7124 was more resistant to Verticillium wilt. A total of 18,138 differentially expressed genes (DEGs), including 1470 transcription factors (TFs). Further analysis of the expression of hormone biosynthesis- and signal transduction-related genes revealed that most of the genes in the salicylic acid (SA), jasmonic acid (JA) and ethylene (ET) biosynthesis pathways were highly expressed in Hai7124; the expression of SA and JA biosynthesis genes began to be significantly upregulated in the early stage of Verticillium wilt stress, while the expression of ET biosynthesis genes was upregulated mainly in the later stage. WGCNA-based binding sequence comparison revealed that MYB14 had nonsynonymous single nucleotide polymorphisms (SNPs) in 5 highly Verticillium wilt-resistant G. barbadense varieties compared to 5 highly Verticillium wilt-susceptible G. hirsutum varieties. Expression analysis revealed that GbMYB14 responded more rapidly to Verticillium wilt stress than the same gene in G. hirsutum. The resistance of G. barbadense and G. hirsutum to Verticillium wilt decreased after MYB14 silencing via virus induced gene silencing (VIGS), and leaf yellowing and necrosis in the GbMYB14-silenced plants were more obvious. Compared with those in G. hirsutum, the expression levels and of lignin biosynthesis pathway genes and the lignin content in GbMYB14-silenced plants were lower. In conclusion, our results provide a theoretical basis for an in-depth understanding of the molecular mechanism underlying the difference in Verticillium wilt resistance between G. barbadense and G. hirsutum and provide a new genetic resource for the study of cotton resistance to Verticillium wilt.

{"title":"Comparative transcriptomic analysis and identification of candidate genes related to Verticillium wilt resistance in Gossypium barbadense and Gossypium hirsutum","authors":"Jieyin Zhao , Xingui Hu , Yuxiang Wang, Jiaxin Lu, Wenju Gao, Xuening Su, Quanjia Chen, Yanying Qu","doi":"10.1016/j.stress.2025.100759","DOIUrl":"10.1016/j.stress.2025.100759","url":null,"abstract":"<div><div>The resistance to Verticillium wilt in <em>Gossypium barbadense</em> is generally greater than that in <em>Gossypium hirsutum</em>, and analyzing the differences in the mechanism and regulatory genes involved in Verticillium wilt resistance between G<em>. barbadense</em> and G<em>. hirsutum</em> is particularly important. Here, we report a transcriptomic study for phenotypic evaluation of Verticillium wilt resistance in G<em>. hirsutum</em> (TM-1) and G<em>. barbadense</em> (Hai7124) and a comparison of the transcriptomes at 7 time points after <em>Verticillium dahliae</em> inoculation. Phenotypic evaluation revealed that, compared with TM-1, Hai7124 was more resistant to Verticillium wilt. A total of 18,138 differentially expressed genes (DEGs), including 1470 transcription factors (TFs). Further analysis of the expression of hormone biosynthesis- and signal transduction-related genes revealed that most of the genes in the salicylic acid (SA), jasmonic acid (JA) and ethylene (ET) biosynthesis pathways were highly expressed in Hai7124; the expression of SA and JA biosynthesis genes began to be significantly upregulated in the early stage of Verticillium wilt stress, while the expression of ET biosynthesis genes was upregulated mainly in the later stage. WGCNA-based binding sequence comparison revealed that MYB14 had nonsynonymous single nucleotide polymorphisms (SNPs) in 5 highly Verticillium wilt-resistant G<em>. barbadense</em> varieties compared to 5 highly Verticillium wilt-susceptible G<em>. hirsutum</em> varieties. Expression analysis revealed that <em>GbMYB14</em> responded more rapidly to Verticillium wilt stress than the same gene in G<em>. hirsutum</em>. The resistance of G<em>. barbadense</em> and G<em>. hirsutum</em> to Verticillium wilt decreased after MYB14 silencing via virus induced gene silencing (VIGS), and leaf yellowing and necrosis in the <em>GbMYB14</em>-silenced plants were more obvious. Compared with those in G<em>. hirsutum</em>, the expression levels and of lignin biosynthesis pathway genes and the lignin content in <em>GbMYB14</em>-silenced plants were lower. In conclusion, our results provide a theoretical basis for an in-depth understanding of the molecular mechanism underlying the difference in Verticillium wilt resistance between G<em>. barbadense</em> and G<em>. hirsutum</em> and provide a new genetic resource for the study of cotton resistance to Verticillium wilt.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"15 ","pages":"Article 100759"},"PeriodicalIF":6.8,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143330786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Small open reading frames (sORFs): Driving big improvements in plant development and quality

IF 6.8 Q1 PLANT SCIENCES

Plant Stress

Pub Date : 2025-02-05 DOI: 10.1016/j.stress.2025.100761

Kui Dong, Chaofan Shan, Dongyu Wen, Zifan Cui, Jun Cao

Small open reading frames (sORFs) are defined as short nucleotide sequences, typically no longer than 300 base pairs, which have the potential to encode small peptides. With the advent of sophisticated technologies such as bioinformatics, ribosome profiling, and mass spectrometry, the investigation of sORFs has experienced a transformative shift and significant expansion. This review provides a comprehensive analysis of the identification, categorization, functional mechanisms, and diverse roles of sORFs in plant development. In the plant, sORFs are pivotal components that enhance responses to both biotic and abiotic stresses by encoding small peptides that are either intricately involved in complex hormone regulation and signaling networks, or precisely regulate the expression of downstream resistance genes. The impact of sORFs extends well beyond stress resistance and they are also implicated in the developmental and physiological functions of plants. sORFs play a crucial role in determing the overall morphological architecture of plants, governing morphological changes throughout the plant life cycle, and are instrumental in regulating metabolite accumulation, which influences plant quality. The synergistic application of sORFs with gene editing technologies holds great promise, not only for devising novel strategies to improve plant quality, resulting in crops that are more resistant, nutrient-rich, and productive, but also for establishing innovative molecular breeding pathways. In summary, understanding and harnessing the potential of sORFs is a cutting-edge area of research that is poised to revolutionize the future of plant biology and agriculture, and ongoing exploration of their functions and mechanisms is anticipated to yield groundbreaking discoveries and practical applications in the future.

{"title":"Small open reading frames (sORFs): Driving big improvements in plant development and quality","authors":"Kui Dong, Chaofan Shan, Dongyu Wen, Zifan Cui, Jun Cao","doi":"10.1016/j.stress.2025.100761","DOIUrl":"10.1016/j.stress.2025.100761","url":null,"abstract":"<div><div>Small open reading frames (sORFs) are defined as short nucleotide sequences, typically no longer than 300 base pairs, which have the potential to encode small peptides. With the advent of sophisticated technologies such as bioinformatics, ribosome profiling, and mass spectrometry, the investigation of sORFs has experienced a transformative shift and significant expansion. This review provides a comprehensive analysis of the identification, categorization, functional mechanisms, and diverse roles of sORFs in plant development. In the plant, sORFs are pivotal components that enhance responses to both biotic and abiotic stresses by encoding small peptides that are either intricately involved in complex hormone regulation and signaling networks, or precisely regulate the expression of downstream resistance genes. The impact of sORFs extends well beyond stress resistance and they are also implicated in the developmental and physiological functions of plants. sORFs play a crucial role in determing the overall morphological architecture of plants, governing morphological changes throughout the plant life cycle, and are instrumental in regulating metabolite accumulation, which influences plant quality. The synergistic application of sORFs with gene editing technologies holds great promise, not only for devising novel strategies to improve plant quality, resulting in crops that are more resistant, nutrient-rich, and productive, but also for establishing innovative molecular breeding pathways. In summary, understanding and harnessing the potential of sORFs is a cutting-edge area of research that is poised to revolutionize the future of plant biology and agriculture, and ongoing exploration of their functions and mechanisms is anticipated to yield groundbreaking discoveries and practical applications in the future.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"15 ","pages":"Article 100761"},"PeriodicalIF":6.8,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143377250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

GmERF57 negatively regulates root development and phosphate absorption in soybean

IF 6.8 Q1 PLANT SCIENCES

Plant Stress

Pub Date : 2025-02-05 DOI: 10.1016/j.stress.2025.100763

Hongqing Zhu , Dandan Hu , Yifei Yang , Xuhao Zhai , Shanshan Zhang , Mengshi He , Huifang Zuo , Lina Zhang , Mengjun Xu , Shanshan Chu , Haiyan Lü , Hengyou Zhang , Yu Zhang , Dan Zhang

Low soil phosphate (Pi) availability is a primary limiting factor for crop growth and production due to its immobilization in soil, thereby impeding Pi uptake by plants. However, excessive supply of phosphorus fertilizer can result in eutrophication of water bodies. Therefore, improving Pi utilization and uptake efficiency in plants is crucial for sustainable agriculture. Previously, we identified soybean Ethylene Response Factor 57 (GmERF57) as a candidate gene responsible for q20, a major QTL associated with soybean low Pi (LP) tolerance-related traits identified through QTL mapping and genome-wide association analysis (GWAS). Population genomics analysis revealed that GmERF57 has undergone artificial selection, and allelic distribution analysis significantly correlated with traits associated with LP tolerance in soybean. Haplotype 2 (Hap2), carrying the T to C single nucleotide polymorphism (SNP), represents the optimal allele favoring LP tolerance. Silencing GmERF57 expression promoted root development and enhanced plant Pi uptake; conversely, overexpression of GmERF57 yielded contrasting phenotypes compared to silenced roots. We further uncovered that GmERF57 physically interacts with GmTUB1 (β-tubulin protein) and modulates soybean root architecture and Pi uptake capacity by downregulating GmTUB1 and other Pi starvation response genes. Overall, the results identified GmERF57 as a QTL gene associated with LP tolerance, elucidated its role in regulating LP tolerance, and further identified an optimal haplotype to facilitate breeding of LP-tolerant soybean cultivars.

{"title":"GmERF57 negatively regulates root development and phosphate absorption in soybean","authors":"Hongqing Zhu , Dandan Hu , Yifei Yang , Xuhao Zhai , Shanshan Zhang , Mengshi He , Huifang Zuo , Lina Zhang , Mengjun Xu , Shanshan Chu , Haiyan Lü , Hengyou Zhang , Yu Zhang , Dan Zhang","doi":"10.1016/j.stress.2025.100763","DOIUrl":"10.1016/j.stress.2025.100763","url":null,"abstract":"<div><div>Low soil phosphate (Pi) availability is a primary limiting factor for crop growth and production due to its immobilization in soil, thereby impeding Pi uptake by plants. However, excessive supply of phosphorus fertilizer can result in eutrophication of water bodies. Therefore, improving Pi utilization and uptake efficiency in plants is crucial for sustainable agriculture. Previously, we identified soybean Ethylene Response Factor 57 (<em>GmERF57</em>) as a candidate gene responsible for <em>q20</em>, a major QTL associated with soybean low Pi (LP) tolerance-related traits identified through QTL mapping and genome-wide association analysis (GWAS). Population genomics analysis revealed that <em>GmERF57</em> has undergone artificial selection, and allelic distribution analysis significantly correlated with traits associated with LP tolerance in soybean. Haplotype 2 (Hap2), carrying the T to C single nucleotide polymorphism (SNP), represents the optimal allele favoring LP tolerance. Silencing <em>GmERF57</em> expression promoted root development and enhanced plant Pi uptake; conversely, overexpression of <em>GmERF57</em> yielded contrasting phenotypes compared to silenced roots. We further uncovered that GmERF57 physically interacts with GmTUB1 (β-tubulin protein) and modulates soybean root architecture and Pi uptake capacity by downregulating <em>GmTUB1</em> and other Pi starvation response genes. Overall, the results identified <em>GmERF57</em> as a QTL gene associated with LP tolerance, elucidated its role in regulating LP tolerance, and further identified an optimal haplotype to facilitate breeding of LP-tolerant soybean cultivars.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"15 ","pages":"Article 100763"},"PeriodicalIF":6.8,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143348782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dynamic changes and transcriptome analyses reveal the microfilament skeleton response to water stress in thalli of Neopyropia yezoensis inhabiting the intertidal zone

IF 6.8 Q1 PLANT SCIENCES

Plant Stress

Pub Date : 2025-02-05 DOI: 10.1016/j.stress.2025.100762

Jiqiang Yin , Ying Sun , Xinping Miao , Jiaxin Qu , Kunjie Zhang , Xue qing Han , Yichi Li , Jiahui Sun , Fanna Kong

The microfilament (MF) cytoskeleton, present in all eukaryotic cells, is not only essential for fundamental cellular processes but also is important in sensing and transducing external signals in response to various developmental cues and abiotic stresses. Neopyropia yezoensis, a species of seaweed belonging to the Rhodophyta, is an important macroalga that thrives in the intertidal zone. However, it remains uncertain whether the MF cytoskeleton of seaweed contributes to adaption to desiccation and rehydration. In this study, we present for the first time the evidence regarding the role of MFs in the desiccation tolerance of N. yezoensis. The organization and arrangement of MFs were significantly influenced by variations in the water content within thallus cells. Desiccation of the thallus induced changes of many actin and actin binding proteins (ABPs) at transcriptional, translational and post-translational phosphorylation levels. Notably, nine phosphosites from four proteins (actin, formin, septin, and fascin) showed changes in phosphorylation conditions. This indicate that phosphorylation modification was involved in MFs response to desiccation and rehydration stress. Transcriptome analysis revealed that Latrunculin A, an MF polymerization inhibitor, significantly suppressed the expression of actin and ABPs genes. Further analysis indicated that MF participates in the responses to desiccation in N. yezoensis by regulating plastid function, ROS levels, phosphorylation modification of proteins, Ca²⁺ signals and vesicle transport processes. Additionally, two MYB transcriptional factors were identified as being induced by regulating the MF cytoskeleton assembly. Finally, we developed a hypothesis concerning the regulation of the microfilament skeleton as a fundamental response to water loss in thalli of N. yezoensis. Our findings will enhance our understanding the adaption mechanisms of N. yezoensis to water stress and broaden our knowledge regarding the response of MF cytoskeleton to water stress. Furthermore, this research will provide valuable insights into the species distribution of intertidal zones.

{"title":"Dynamic changes and transcriptome analyses reveal the microfilament skeleton response to water stress in thalli of Neopyropia yezoensis inhabiting the intertidal zone","authors":"Jiqiang Yin , Ying Sun , Xinping Miao , Jiaxin Qu , Kunjie Zhang , Xue qing Han , Yichi Li , Jiahui Sun , Fanna Kong","doi":"10.1016/j.stress.2025.100762","DOIUrl":"10.1016/j.stress.2025.100762","url":null,"abstract":"<div><div>The microfilament (MF) cytoskeleton, present in all eukaryotic cells, is not only essential for fundamental cellular processes but also is important in sensing and transducing external signals in response to various developmental cues and abiotic stresses. <em>Neopyropia yezoensis</em>, a species of seaweed belonging to the Rhodophyta, is an important macroalga that thrives in the intertidal zone. However, it remains uncertain whether the MF cytoskeleton of seaweed contributes to adaption to desiccation and rehydration. In this study, we present for the first time the evidence regarding the role of MFs in the desiccation tolerance of <em>N. yezoensis</em>. The organization and arrangement of MFs were significantly influenced by variations in the water content within thallus cells. Desiccation of the thallus induced changes of many actin and actin binding proteins (ABPs) at transcriptional, translational and post-translational phosphorylation levels. Notably, nine phosphosites from four proteins (actin, formin, septin, and fascin) showed changes in phosphorylation conditions. This indicate that phosphorylation modification was involved in MFs response to desiccation and rehydration stress. Transcriptome analysis revealed that Latrunculin A, an MF polymerization inhibitor, significantly suppressed the expression of actin and ABPs genes. Further analysis indicated that MF participates in the responses to desiccation in <em>N. yezoensis</em> by regulating plastid function, ROS levels, phosphorylation modification of proteins, Ca<sup>2+</sup> signals and vesicle transport processes. Additionally, two MYB transcriptional factors were identified as being induced by regulating the MF cytoskeleton assembly. Finally, we developed a hypothesis concerning the regulation of the microfilament skeleton as a fundamental response to water loss in thalli of <em>N. yezoensis</em>. Our findings will enhance our understanding the adaption mechanisms of <em>N. yezoensis</em> to water stress and broaden our knowledge regarding the response of MF cytoskeleton to water stress. Furthermore, this research will provide valuable insights into the species distribution of intertidal zones.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"15 ","pages":"Article 100762"},"PeriodicalIF":6.8,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143387617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Maximizing nitrogen stress tolerance through high-throughput phenotyping in rice

IF 6.8 Q1 PLANT SCIENCES

Plant Stress

Pub Date : 2025-02-05 DOI: 10.1016/j.stress.2025.100764

Nguyen Trung Duc , Amooru Harika , Dhandapani Raju , Sudhir Kumar , Renu Pandey , Ranjith Kumar Ellur , Gopala Krishnan S , Elangovan Allimuthu , Biswabiplab Singh , Ayyagari Ramlal , Ambika Rajendran , Ranjeet Ranjan Kumar , Madan Pal Singh , Rabi Narayan Sahoo , Viswanathan Chinnusamy

Nitrogen (N) is a significant nutrient element limiting rice yield and quality, a major staple crop consumed worldwide. N deficiency negatively affects the growth and development of rice by impacting vital physiological processes. Plants have developed multiple resilience strategies, including enhanced nitrogen use efficiency (NUE) to cope with N-deprived situations. NUE in rice is less than 40 %, and increased N application leads to high production costs and ecosystem damage. Improving NUE has been one of the major challenges of agriculture research in the recent past. NUE is an obfuscated trait governed by diverse physiological traits and controlled by complex genetic mechanisms. In recent years, a combination of multi-omics techniques (phenomics and genomics) has enhanced the N resilience maximization efforts of the agricultural research community. Phenomics technology has displayed the ability to perform systematic, organism-wide phenotyping of N stress response in diverse crops over the entire life cycle using non-invasive sensors on high throughput platforms (HTPs) in a more precise manner. These HTPs augment precision phenotyping (at the spatiotemporal scale) of component traits of NUE, which are difficult to phenotype mainly due to its dynamic interactive nature with the environment. Phenomics has drastically reduced the phenotype-genotype gap by optimally utilising other omics data for breeding climate smart cultivars with enhanced N stress tolerance. This review focuses on the recent advances in HTP-based phenotyping of NUE-related traits to identify novel QTLs/genes/signaling pathways associated with improved NUE both in controlled environments and field conditions.

{"title":"Maximizing nitrogen stress tolerance through high-throughput phenotyping in rice","authors":"Nguyen Trung Duc , Amooru Harika , Dhandapani Raju , Sudhir Kumar , Renu Pandey , Ranjith Kumar Ellur , Gopala Krishnan S , Elangovan Allimuthu , Biswabiplab Singh , Ayyagari Ramlal , Ambika Rajendran , Ranjeet Ranjan Kumar , Madan Pal Singh , Rabi Narayan Sahoo , Viswanathan Chinnusamy","doi":"10.1016/j.stress.2025.100764","DOIUrl":"10.1016/j.stress.2025.100764","url":null,"abstract":"<div><div>Nitrogen (N) is a significant nutrient element limiting rice yield and quality, a major staple crop consumed worldwide. N deficiency negatively affects the growth and development of rice by impacting vital physiological processes. Plants have developed multiple resilience strategies, including enhanced nitrogen use efficiency (NUE) to cope with N-deprived situations. NUE in rice is less than 40 %, and increased N application leads to high production costs and ecosystem damage. Improving NUE has been one of the major challenges of agriculture research in the recent past. NUE is an obfuscated trait governed by diverse physiological traits and controlled by complex genetic mechanisms. In recent years, a combination of multi-omics techniques (phenomics and genomics) has enhanced the N resilience maximization efforts of the agricultural research community. Phenomics technology has displayed the ability to perform systematic, organism-wide phenotyping of N stress response in diverse crops over the entire life cycle using non-invasive sensors on high throughput platforms (HTPs) in a more precise manner. These HTPs augment precision phenotyping (at the spatiotemporal scale) of component traits of NUE, which are difficult to phenotype mainly due to its dynamic interactive nature with the environment. Phenomics has drastically reduced the phenotype-genotype gap by optimally utilising other omics data for breeding climate smart cultivars with enhanced N stress tolerance. This review focuses on the recent advances in HTP-based phenotyping of NUE-related traits to identify novel QTLs/genes/signaling pathways associated with improved NUE both in controlled environments and field conditions.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"15 ","pages":"Article 100764"},"PeriodicalIF":6.8,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143419836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome-wide association analysis for pollen viability under heat stress in peanut

IF 6.8 Q1 PLANT SCIENCES

Plant Stress

Pub Date : 2025-02-03 DOI: 10.1016/j.stress.2025.100760

Hrishikesh P. Ingole , Gautam Saripalli , Zachary T. Jones , Manikannan Parthiban , Johnson Toyinbo , Sruthi Narayanan , Reyazul Rouf Mir , Sachin Rustgi

Peanut is one of the most important crops, providing nutrition and food security to millions worldwide. Pollen viability (PV) is a major determinant of yield in crops and is adversely impacted by heat stress. This study aimed to identify molecular markers associated with sustained PV under heat stress. We evaluated 72 genetically non-redundant genotypes from the U.S. peanut mini-core collection for PV under heat stress and compared them to the PV of "Georgia Green," a runner-type peanut variety used as a control. Seventy-two genotypes were grown under optimal conditions (28/22 °C day/night temperatures with a 16 h photoperiod). Once the plants reached the five-leaf stage, heat stress was applied for two weeks by raising the daytime temperature to 38 °C and the nighttime temperature to 28 °C. Un-opened flowers were collected and assayed for PV through in vitro pollen germination. PI 200441 from Japan exhibited the highest PV, while PI 504614 from Colombia showed the lowest. A genome-wide association study for PPV (percent pollen viability) under heat stress identified a marker on chromosome 20. Haplotype analysis revealed a 6 kb region, designated qPPVA20, containing three candidate genes, two of which (ribosomal protein and copper-transporting ATPase) showed high expression in reproductive organs. The co-localization of qPPVA20 with a previously reported QTL hotspot for heat stress-related traits makes these genes important targets for future validation. Markers associated with seed lipid compositional traits, such as behenate, arachidate, oleate, and eicosenoate content under optimal growth conditions, were also identified, with plans to investigate the impact of heat stress on these QTLs in a future study.

{"title":"Genome-wide association analysis for pollen viability under heat stress in peanut","authors":"Hrishikesh P. Ingole , Gautam Saripalli , Zachary T. Jones , Manikannan Parthiban , Johnson Toyinbo , Sruthi Narayanan , Reyazul Rouf Mir , Sachin Rustgi","doi":"10.1016/j.stress.2025.100760","DOIUrl":"10.1016/j.stress.2025.100760","url":null,"abstract":"<div><div>Peanut is one of the most important crops, providing nutrition and food security to millions worldwide. Pollen viability (PV) is a major determinant of yield in crops and is adversely impacted by heat stress. This study aimed to identify molecular markers associated with sustained PV under heat stress. We evaluated 72 genetically non-redundant genotypes from the U.S. peanut mini-core collection for PV under heat stress and compared them to the PV of \"Georgia Green,\" a runner-type peanut variety used as a control. Seventy-two genotypes were grown under optimal conditions (28/22 °C day/night temperatures with a 16 h photoperiod). Once the plants reached the five-leaf stage, heat stress was applied for two weeks by raising the daytime temperature to 38 °C and the nighttime temperature to 28 °C. Un-opened flowers were collected and assayed for PV through <em>in vitro</em> pollen germination. PI 200441 from Japan exhibited the highest PV, while PI 504614 from Colombia showed the lowest. A genome-wide association study for PPV (percent pollen viability) under heat stress identified a marker on chromosome 20. Haplotype analysis revealed a 6 kb region, designated <em>qPPVA20</em>, containing three candidate genes, two of which (ribosomal protein and copper-transporting ATPase) showed high expression in reproductive organs. The co-localization of <em>qPPVA20</em> with a previously reported QTL hotspot for heat stress-related traits makes these genes important targets for future validation. Markers associated with seed lipid compositional traits, such as behenate, arachidate, oleate, and eicosenoate content under optimal growth conditions, were also identified, with plans to investigate the impact of heat stress on these QTLs in a future study.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"15 ","pages":"Article 100760"},"PeriodicalIF":6.8,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143419835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0