Plant Stress最新文献

{"title":"ABA deficiency and its impact on ion and metabolite profiles in tomato roots under single and combined stress conditions","authors":"Miriam Pardo-Hernández ,&nbsp;Pascual García-Pérez ,&nbsp;Luigi Lucini ,&nbsp;Rosa M Rivero","doi":"10.1016/j.stress.2024.100644","DOIUrl":"10.1016/j.stress.2024.100644","url":null,"abstract":"<div><div>Abscisic acid (ABA) plays a crucial role in the stress response of plants. Although the impact of ABA on individual stresses has been extensively studied, there is less research on its role in plants grown under stress combination, such as salinity and high temperature. This study analyzes the response at the ionomic and metabolomic levels of tomato roots to ABA deficiency under single salinity or heat stress, as well as under the combination of both stresses, using ABA-deficient (<em>flacca, flc</em>) tomato plants. ABA was found to be crucial in ionic regulation, particularly for Na, K, Ca, and other ions such as Cu, Mn, Zn, Mo, and Fe. However, its influence depended on the type of stress applied, indicating the complexity of plant responses to these adverse environmental factors. In our study, phenylpropanoids, terpenoids, and nitrogenous compounds were the metabolites most affected by endogenous ABA concentration and environmental treatment. On the other hand, the application of exogenous ABA to <em>flc</em> mutants did not fully restore root dry weight, although it did cause significant changes at the ionomic and metabolomic levels. Salinity and heat applied in combination aggravated the vulnerability of <em>flc</em> mutants compared to single stresses, making the application of exogenous ABA more effective under single stresses than under the combined stresses. Finally, a correlation analysis between the ionome and metabolome revealed that the accumulation or deficiency of some ions (i.e., Na, Zn, and Fe) was correlated with the abundance of important metabolites related to amino acid biosynthesis and terpenoid metabolism, among others.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"14 ","pages":"Article 100644"},"PeriodicalIF":6.8,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142539176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Relationships between freezing resistance and biochemicals in grapevine buds and canes: Different soluble carbohydrates accumulate in several cultivars during cold acclimation","authors":"Yutaro Kita,&nbsp;Takashi Suzuki,&nbsp;Yutaka Jitsuyama","doi":"10.1016/j.stress.2024.100639","DOIUrl":"10.1016/j.stress.2024.100639","url":null,"abstract":"<div><div>Owing to recent warming trends during the growing season, wine grapevine (<em>Vitis vinifera</em>) production has increased in subarctic areas such as Hokkaido. However, high freezing resistance remains essential for grapevines due to the severe winter temperatures in these regions. Here, we investigated the seasonal variation in freezing resistance, as well as the water, soluble carbohydrate, proline, and total phenolic content (TPC) in the buds and canes of two <em>V. vinifera</em> cultivars and a <em>Vitis riparia</em> hybrid, Maeve. We found that freezing resistance in the buds and canes increased from fall to winter, with Maeve exhibiting higher freezing resistance than the <em>V. vinifera</em> cultivars during both seasons. Maeve experienced a faster rate of deacclimation, leading to comparable spring freezing resistance among all cultivars. The water content in the buds and canes of all three cultivars decreased during the fall, with Maeve showing an earlier reduction compared to the others. Most soluble carbohydrates increased in winter and decreased in spring. Notably, Maeve, which had higher winter freezing resistance, accumulated more sucrose in its buds and a higher concentration of soluble carbohydrates, particularly monosaccharides, in its canes compared to the other two cultivars. Proline and TPC levels were not linked to freezing resistance. Grapevine buds and canes appeared to accumulate soluble carbohydrates, especially monosaccharides, during winter, while reducing water content in fall to enhance freezing resistance. This study suggests that grapevine cultivars in Hokkaido may utilize different types of soluble carbohydrates to improve the freezing resistance of their buds and canes.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"14 ","pages":"Article 100639"},"PeriodicalIF":6.8,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142539754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Graphene oxide improves the tolerance of Tartary buckwheat to continuous cropping by coordinating the antioxidant defense system and endogenous hormone levels","authors":"Yu Zhang ,&nbsp;Xiaoyan Huang ,&nbsp;Kaifeng Huang","doi":"10.1016/j.stress.2024.100646","DOIUrl":"10.1016/j.stress.2024.100646","url":null,"abstract":"<div><div>Continuous cropping obstacles seriously restrict the yield of Tartary buckwheat. Graphene oxide (GO) is shown to play an important role in defense mechanism to plant abiotic stress. However, the relationship between GO and improving continuous cropping tolerance is unclear. The main objective of this study was to analyze the physiological mechanism of GO in alleviating continuous cropping injury. A 2-year field experiment was conducted on Tartary buckwheat (Jinqiao 2) to investigate the characteristics with five GO treatments, namely, 0 (control), 25 (G₁), 50 (G₂), 100 (G₃), and 200 mg L^–1 (G₄). With increasing the GO application concentration, the rhizosphere soil nutrient content, root morphology and activity, photosynthetic characteristics and chlorophyll content, antioxidant enzyme activities, osmotica and auxin (IAA) content, agronomic traits, and yield initially increased and then decreased. The contents of malonaldehyde, superoxide anion free radical, and abscisic acid (ABA) initially decreased and then increased. Compared with CK, the yields subjected to continuous cropping and treated with G₁, G₂, G₃, and G₄ were 1.28, 1.58, 1.43, and 1.16 times, respectively. GO treatment, especially G₂ treatment, can improve the tolerance and increase the yield of Tartary buckwheat to continuous cropping, and it is suitable for widespread use by farmers in Tartary buckwheat production.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"14 ","pages":"Article 100646"},"PeriodicalIF":6.8,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142539172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of Dynamin-related proteins (DRP) in bread wheat: TaDRP1D-B as regulator of biotic and abiotic stresses","authors":"Zhiwei Wang ,&nbsp;Aimen Shafique ,&nbsp;Bofeng Yu ,&nbsp;Badr Alharthi ,&nbsp;Naushad Ali ,&nbsp;Muhammad Salman Mubarik ,&nbsp;Hafiz Saeed ur Rehman ,&nbsp;Rashid Iqbal ,&nbsp;Farrukh Azeem ,&nbsp;Hongxing Xu","doi":"10.1016/j.stress.2024.100645","DOIUrl":"10.1016/j.stress.2024.100645","url":null,"abstract":"<div><div>Bread wheat (<em>Triticum aestivum</em>) is a vital global staple food, providing 30 % of the world's caloric intake and nutritional needs. It was domesticated over 10,000 years ago and adapted to various biotic and abiotic stresses, crucial for maintaining food security. Modern research highlights the interconnected signalling pathways for both biotic and abiotic stresses, that help wheat cope with these biotic and abiotic stresses. Identification of regulatory proteins is essential for advanced wheat breeding. In the current study, 32 <em>DRP</em> genes in wheat were identified that are evenly distributed on all the chromosomes with the presence of conserved dynamin-related domain. PPI analysis reveals that the <em>TaDRP2</em>-like genes interact with each other. Gene ontology analysis indicating the significant involvement of <em>DRP</em> genes in various processes including GTPase activity, binding, microtubule binding, and various cells including membrane (GO:0,016,020), cytoplasm (GO:0,005,737), microtubule (GO:0,005,874). Cis-element prediction reveals the enrichment of total 2006 elements including CAAT-box (390), TATA-box (327), MYB (131), and ABRE (93). Transcriptome and qRT-PCR analyses showed that TaDRP1-like, TaDRP2-like, and TaDRP3-like genes are highly expressed in roots, stems, leaves, and spikes, with lower expression in grains. Notably, TaDRP1D-B emerged as a potential candidate for enhancing resistance to powdery mildew, rust, drought, and heat stress. Furthermore, the interaction compatibility of <em>TaDRP1D-B</em> with PPA2 further confirms the potential role in regulating plant disease response. This research provides a foundation for developing strategies to enhance wheat resilience, directly contributing to global food security.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"14 ","pages":"Article 100645"},"PeriodicalIF":6.8,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142539750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cultivating resilience: Use of water deficit to prime peanut production and improve water stress tolerance","authors":"Aline de Camargo Santos ,&nbsp;Bruce Schaffer ,&nbsp;Diane Rowland ,&nbsp;Matthew Bremgartner ,&nbsp;Pamela Moon ,&nbsp;Barry Tillman ,&nbsp;Elias Bassil","doi":"10.1016/j.stress.2024.100637","DOIUrl":"10.1016/j.stress.2024.100637","url":null,"abstract":"<div><div>Regulated deficit irrigation is a potential strategy for priming peanut plants to improve their acclimation to water stress. To assess possible effects of priming and develop an effective water stress priming strategy, greenhouse experiments were conducted to compare primed and non-primed peanut cultivars, C7616 and TUFRunner ‘511′™, to subsequent water stress. Plants were divided into 1) controls with daily irrigation to field capacity throughout the entire growth cycle, 2) non-primed plants receiving daily irrigation up to 55 to 65 days after planting (DAP), followed by exposure to mid-season water stress, and 3) primed plants that received 50 % of the control irrigation either from 5 to 45 DAP (long-term priming), or 20–45 DAP (short-term priming), followed by mid-season water stress at 55 to 65 DAP. An automated physiological phenotyping platform was used to control irrigation and continuously monitor soil water content, whole-plant transpiration and water use. Single-leaf measurements of net CO₂ assimilation, stomatal conductance, and transpiration were taken periodically. Plant biomass and biomass partitioning were also determined. Results indicated that primed plants grown under water deficit exhibited either reduced (acclimated) or intensified (sensitized) physiological stress responses upon subsequent water stress. Timing and duration of the priming period played a key role in modulating plant phenotypic plasticity, which varied by genotype, suggesting that priming could be in part genetically controlled.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"14 ","pages":"Article 100637"},"PeriodicalIF":6.8,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142554820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exogenous Bacillus subtilis can reduce the damage caused by waste drilling fluid to ryegrass (Lolium perenne)","authors":"Lu Yang,&nbsp;Yuandong Zhu,&nbsp;Heng Zhao,&nbsp;Xiaohu Chen,&nbsp;Zihan Cheng,&nbsp;Yongjun Fei","doi":"10.1016/j.stress.2024.100641","DOIUrl":"10.1016/j.stress.2024.100641","url":null,"abstract":"<div><div>Oil and gas drilling waste fluid are an alkaline mixture with complex composition that can be hazardous to plants if leakage occurs during transportation and disposal. <em>Bacillus subtilis</em> is well-known for its adaptable to adversity and its beneficial effect on plants and soil. In this study, the novel ultra-slippery water-based drilling fluids were evaluated as potentially hazardous liquids capable of inhibiting ryegrass (<em>Lolium perenne</em>) germination and growth. However, the combination of ryegrass and <em>B. subtilis</em> successfully decreased the negative effects of waste drilling fluid stress, while increasing the levels of antioxidant enzymes and osmotic regulatory substances, resulting in improved ryegrass germination and growth. Furthermore, <em>B. subtilis</em> enhanced the activation of nitrogen, phosphorus, and potassium in the soil, which improved soil conditions and promoted ryegrass development. This study proposes a novel approach for combined remediation of waste drilling fluid pollution in oil and gas drilling sites using microbial agents and plants, while also furnishing resources for enhancing ryegrass resilience and facilitating ecological restoration.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"14 ","pages":"Article 100641"},"PeriodicalIF":6.8,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142539735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"\"Moringa leaf extract alleviates salt stress in tomato (Solanum lycopersicum L.) by activating antioxidant defenses, reducing osmolyte accumulation, improving water status, and enhancing yield.\"","authors":"Adewale Suraj Bello,&nbsp;Talaat Ahmed","doi":"10.1016/j.stress.2024.100640","DOIUrl":"10.1016/j.stress.2024.100640","url":null,"abstract":"<div><div>Salt stress poses a significant threat to crop productivity, prompting investigations into mitigation strategies. This study assessed the efficacy of foliar spraying with moringa leaf extract (MLE) to alleviate salt stress at concentrations of 50, 100, 150, and 200 mM, alongside a control (0 mM), in tomato plants. Salt stress-induced marked significant reductions in vegetative parameters (shoot height, root length, stem diameter, and the number of leaves plant^-1), yet MLE-treated plants exhibited enhanced growth compared to the untreated and control plants. Salinity adversely affected chlorophyll content, relative water content, and membrane stability index, all of which were significantly ameliorated by MLE application. Additionally, MLE treatment bolstered the activity of catalase, peroxidase, superoxide dismutase, and proline accumulation, contributing to improved plant resilience. Notably, MLE-treated plants demonstrated significantly increased yields compared to untreated counterparts. These findings underscore the potential of MLE as a bioresource for mitigating salt stress in tomato plants, offering promising avenues for sustainable crop management in the highly saline soil in a region like Qatar.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"14 ","pages":"Article 100640"},"PeriodicalIF":6.8,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142539170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From grass to yeast; functional insights from heterologous expression of LfHKT2;1 in ion regulation","authors":"Khurram Shahzad ,&nbsp;Muhammad Rauf ,&nbsp;Sher Aslam Khan ,&nbsp;Attiq ur Rehman ,&nbsp;Modassir Ahmed ,&nbsp;Badr Alharthi ,&nbsp;Shah Fahad ,&nbsp;Nasir A. Saeed","doi":"10.1016/j.stress.2024.100634","DOIUrl":"10.1016/j.stress.2024.100634","url":null,"abstract":"<div><div><em>Saccharomyces cervisceae</em> mutants lacking the major potassium trasnporters trk1 and trk2 show hypersensitivity to aminoglycoside antibiotic hygromycin (hyg). This study demonstrates that expression of the inward K⁺ transporter <em>LfHKT2;1</em> can suppress this hygromycin sensitivity in the <em>trk1, trk2</em> double mutant strain. Growth complementation was performed on solid yeast peptone dextrose (YPD) media supplemented with hygromycin B. Both, wild type and <em>trk1 trk2</em> yeast strains exhibited growth inhibition in the presence of hygromycin. However the potassium uptake-deficient trk1 trk2 strain (control) showed complete growth arrest when exposed to hygromycin, while expression of <em>LfHKT2;1</em> resulted in growth recovery. Increased sodium concentrations caused cellular toxicity in the <em>trk1 trk2</em> strain, which was exacerbated by the addition of hygromycin to the media. The hypersensitivity of <em>trk1 trk2</em> mutant yeast cells expressing <em>LfHKT2;1</em> to sodium suggested the presence of an additional sodium uptake system on the membrane, which was further confirmed by transient GFP expression assays. These results provide conclusive evidence that heterologous expression of <em>LfHKT2;1</em> confers both sodium and K⁺ uptake capabilities in hygromycin supplemented YPD media, thereby rescuing the growth of K⁺ transport-deficient <em>S. cerevisiae</em> mutants. This highlights the potential of plant gene expression in yeast as a valuable tool for studying ion transport mechanisms and gene function under stress conditions.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"14 ","pages":"Article 100634"},"PeriodicalIF":6.8,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142539751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing tomato growth and soil fertility under salinity stress using halotolerant plant growth-promoting rhizobacteria","authors":"Ning Yan,&nbsp;Weichi Wang,&nbsp;Tong Mi,&nbsp;Xuefeng Zhang,&nbsp;Xinyue Li,&nbsp;Guodong Du","doi":"10.1016/j.stress.2024.100638","DOIUrl":"10.1016/j.stress.2024.100638","url":null,"abstract":"<div><div>Soil salinization is a critical issue that not only hampers the efficiency and sustainability of global agricultural production but also poses significant challenges to the achievement of sustainable development goals across environmental, economic, and social dimensions. Halotolerant plant growth-promoting rhizobacteria (HPGPR) have the potential to mitigate abiotic stress, foster plant growth, and bolster the stress resistance capabilities of crops. This study conducted the isolation, identification, and characterization of HPGPR originating from a saline-alkali orchard area in northwest China. The efficacy of the isolated bacterial strains was evaluated through potted plant experiments, assessing the growth of tomato plants under in vitro conditions and under varying salinity stress. Ultimately, the study investigated the influence of these HPGPR on soil physicochemical properties, enzymatic activities, and the structure and composition of the microbial community. Upon isolating 12 bacterial strains, we conducted an in vitro assessment of their salt tolerance, ultimately singling out three robust isolates, which exhibited exceptional salt tolerance. Detailed 16S rRNA gene sequencing and meticulous taxonomic evaluation systematically assigned these isolates to <em>Priestia endophyticus</em> GSCK1 (accession number: OR569048), <em>Bacillus atrophaeus</em> GSCK2 (accession number: OR569061), and <em>Serratia fonticola</em> GSCK6 (accession number: OR569062), respectively. These strains exhibited notable biochemical and plant growth-promoting traits, including enzymatic activities and the production of indole-3-acetic acid. They significantly enhanced plant growth metrics and soil fertilities, particularly strain GSCK6, which also reshaped the soil microbial community, augmenting beneficial microbe abundance. The HPGPR treatment notably improved soil pH, nutrient availability, enzymatic activities, and reduced soil electrical conductivity, underscoring their potential in agricultural resilience against salinity. The eco-friendly salt stress mitigation strategy of HPGPR not only enhances soil quality and promotes plant growth by regulating the composition and function of microbial communities, but also provides a novel solution for global agricultural production. This approach is conducive to increasing crop yield and quality, reducing the limitations of saline-alkali land on agricultural production, and promoting food security and sustainable agricultural development.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"14 ","pages":"Article 100638"},"PeriodicalIF":6.8,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142539175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mitigation of microplastic toxicity in soybean by synthetic bacterial community and arbuscular mycorrhizal fungi interaction: Altering carbohydrate metabolism, hormonal transduction, and genes associated with lipid and protein metabolism","authors":"Muhammad Asad ,&nbsp;Zeeshan Khan ,&nbsp;Tariq Shah ,&nbsp;Muhammad Abdullah Shah ,&nbsp;Ayesha Imran ,&nbsp;Salman Rasool ,&nbsp;Jabar Zaman Khan Khattak ,&nbsp;Shah Rukh Khan ,&nbsp;Ajaz Ahmad ,&nbsp;Parvaiz Ahmad","doi":"10.1016/j.stress.2024.100631","DOIUrl":"10.1016/j.stress.2024.100631","url":null,"abstract":"<div><div>Microplastics (MPs) have become ubiquitous environmental pollutants, eliciting concerns about their negative impacts on terrestrial and agroecosystems. However, employing sustainable remediation approaches holds the potential to mitigate these negative impacts and bolster MPs tolerance in both the seeds and vegetative structures of plants. The current investigation explores the impact of arbuscular mycorrhizal fungi (AMF) and synthetic bacterial community (SynCom) as potential alleviators of MP-induced stress. Results exhibited that co-applied SynCom and AMF, resulted in the highest plant biomass, leaf area, pod per plant, and 100-seed weight under control (no MPs) and MPs stress. The combined application of SynCom and AMF enhanced colonization, association, and arbuscule abundance in the non-stressed plants (no MPs) as compared to stressed plants. The sole or co-application of SynCom and AMF enhanced the primary metabolites content and mitigated the MPs-induced reduction in soluble sugars, lipids, protein, and oil contents. Soybean inoculation stimulated the genes expression involved in lipid and protein biosynthesis, while a contradictory drift was noted for genes associated with lipid and protein degradation, underpinning the observed increment in protein and lipid content. Soybean inoculated with AMF exhibited the utmost α-amylase and β-amylase activities, demonstrating enhanced osmolyte (soluble sugar) production, mainly under MPs stress. Remarkably, sole or co-application further reinforces the positive effect of MPs stress on the osmoprotectants and antioxidant levels, for instance, phenol, flavonoid, glycine betaine contents, and glutathione-S-transferase (GST) activities. Subsequent to the stress release, a stress hormones known as abscisic acid (ABA) reduced in the seeds of inoculated soybean while gibberellin (GA), trans-zeatin riboside (ZR), and indole acetic acid (IAA) were increased. Therefore, it is concluded that the combined application of SynCom and AMF protected the soybean plants from MPs-induced oxidative damage by upregulating osmoprotectants and antioxidant levels. This study provides insights into the potential of sustainable remediation approaches in alleviating the complex impact of MPs on soybean, contributing to future strategies for environmental sustainability. Future studies could explore the optimization of SynCom and AMF formulations for different crops and environmental conditions, potentially leading to practical applications in sustainable agriculture and pollution management.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"14 ","pages":"Article 100631"},"PeriodicalIF":6.8,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142539177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}