Plant Stress最新文献

{"title":"Genome-Wide identification, phylogenetic analysis, and expression of CONSTANS-Like genes in Rosa chinensis in response to Tetranychus urticae infestation","authors":"Yingjie Yang ,&nbsp;Min Tian ,&nbsp;Ziming Shi ,&nbsp;Peifei Zhao ,&nbsp;Yanfei Cai","doi":"10.1016/j.stress.2025.100755","DOIUrl":"10.1016/j.stress.2025.100755","url":null,"abstract":"<div><div>CONSTANS-Like genes play critical roles in plant responses to both biotic and abiotic stresses, but their functions in <em>Rosa chinensis</em> remain poorly understood. In this study, we conducted a genome-wide investigation of <em>COL</em> genes in <em>R. chinensis</em>, identifying 18 genes, designated from <em>RcCOL_1</em> to <em>RcCOL_18</em>. Phylogenetic analysis revealed that these genes were unevenly distributed across three subfamilies (<em>RcCOL_I</em> to <em>RcCOL_III</em>), with <em>RcCOL_III</em> containing the most members. Interspecies evolutionary analysis of 14 Rosaceae species showed that <em>COL</em> genes presented across all these species, though the number of genes varied. Cis-acting element analysis indicated that the <em>RcCOL</em> genes mainly contained elements related to biotic and abiotic stress, light response, as well as growth and development, with <em>RcCOL_4/_13/_14/_15, RcCOL_8/_17/_18</em>, and <em>RcCOL_5/_6/_16</em> showing highly consistent element distributions. Expression pattern analysis under red spider (<em>Tetranychus urticae</em>) attack revealed that subfamilies I and II of the <em>COL</em> gene family play a primary role in the regulation of plant response. Quantitative real-time polymerase chain reaction (qRT-PCR) verification showed that nearly all <em>RcCOL</em> genes were highly expressed following red spider infestation; however, these over-expressed genes were suppressed after introducing predatory mite (<em>Neoseiulus californicus</em>). This indicates that the <em>RcCOL</em> genes may play significant roles in the biotic stress response in <em>R. chinensis</em>. Correlation analysis of <em>RcCOL</em> genes with secondary metabolites demonstrated strong associations. For instance, <em>RcCOL_1</em> and <em>RcCOL_2</em> were highly correlated with geranylacetone, periplogenin, and syringetin, while <em>RcCOL_3</em> and <em>RcCOL_12</em> showed strong correlations with N-acetyltryptamine, uridine 5′-monophosphate, and isorhamnetin. Additionally, <em>RcCOL_7</em> was highly correlated with L‑serine and 3-methylxanthine, and <em>RcCOL_10</em> with byakangelicin, hippuric acid, and nicotineamide. <em>RcCOL_14</em> and <em>RcCOL_15</em> were strongly correlated with metabolites such as pidotimod, vinorelbine tartrate, and okadaic acid. These findings suggest that <em>RcCOL</em> genes modulate secondary metabolite production and play critical roles in the biotic stress response of plants.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"15 ","pages":"Article 100755"},"PeriodicalIF":6.8,"publicationDate":"2025-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143148866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of epigenetics in plant pathogens interactions under the changing environments: A systematic review","authors":"Wenhao Wu ,&nbsp;Guoqiang Fan","doi":"10.1016/j.stress.2025.100753","DOIUrl":"10.1016/j.stress.2025.100753","url":null,"abstract":"<div><div>Epigenetic modifications are emerging as pivotal regulatory mechanisms in plant-pathogen interactions, especially under changing environmental conditions. This review systematically examines the application of epigenetic processes (DNA methylation, histone modifications, non-coding RNAs, and RNA methylation) in shaping the intricate dynamics between plants and pathogens. DNA methylation influences plant immunity by modulating gene expression and chromatin accessibility, while histone modifications, such as acetylation and methylation, enable plants to establish robust defenses by fine-tuning stress-responsive genes. Small RNAs (miRNAs) and long non-coding RNAs (lncRNAs) act as post-transcriptional regulators, orchestrating immune signaling and pathogen counter-defense strategies. RNA methylation, particularly N6-methyladenosine (m6A), adds another layer of regulation, controlling the stability and translation of transcripts involved in host immunity and pathogen virulence. The review highlights the co-evolutionary arms race between plants and pathogens, where pathogens manipulate host epigenetic machinery to suppress defenses, while plants deploy countermeasures to maintain immune resilience. Advances in high-throughput sequencing and CRISPR-based tools are shedding light on these complex interactions, offering opportunities to engineer crops with enhanced disease resistance. Furthermore, the review emphasizes the need to explore the interplay between these epigenetic mechanisms under multifaceted environmental stresses. By integrating recent findings, this review underscores the potential of epigenetic research to revolutionize crop protection strategies, ensuring agricultural sustainability in a rapidly changing world.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"15 ","pages":"Article 100753"},"PeriodicalIF":6.8,"publicationDate":"2025-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143149375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anther transcriptomes in cold-tolerant rice cultivars tend to show insensitive responses","authors":"Koichi Yamamori ,&nbsp;Seiya Ishiguro ,&nbsp;Kei Ogasawara ,&nbsp;Kayyis Muayadah Lubba ,&nbsp;Kaien Fujino ,&nbsp;Kazumitsu Onishi ,&nbsp;Yutaka Sato ,&nbsp;Yuji Kishima","doi":"10.1016/j.stress.2024.100700","DOIUrl":"10.1016/j.stress.2024.100700","url":null,"abstract":"<div><div>Many studies of stress tolerance in plants have characterized genes that show differences among a small number of lines with clearly distinct tolerance or sensitivity to the given stress. From the few cloned genes, it is difficult to genetically interpret intermediate tolerance or susceptibility levels and explain the complexity of stress responses and tolerance. In this study, we explored the changes in the transcriptome of anthers from 13 rice lines with different cold tolerance grown under control conditions or exposed to 4 days of cold stress to look for correlations between cold tolerance at the booting stage and expression levels. When examining the overall expression patterns in anthers at low temperature, the cold-tolerant lines tended to have relatively few highly expressed genes, and the expression levels of ribosome-related genes tended to be lower in cold-tolerant lines than in cold-sensitive lines. Importantly, we observed these different expression patterns between the cold-tolerant and -sensitive lines regardless of whether cold stress had been applied. Minimal changes in genes and repetitive sequences under cold stress also tended to be characteristic of the cold-tolerant lines. We also identified unknown genes whose expression was cold responsive and common to all the lines studied. The rice lines whose transcriptome remains constant or insensitive in response to cold stress are more tolerant to low-temperature exposure during the booting stage than rice lines with more widespread expression changes.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"15 ","pages":"Article 100700"},"PeriodicalIF":6.8,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143098945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Translational research progress and challenges for developing drought resilient rice","authors":"Dinesh Kumar Saini ,&nbsp;Kirti Bardhan ,&nbsp;Impa Somayanda ,&nbsp;Rajeev N. Bahuguna ,&nbsp;S.V. Krishna Jagadish","doi":"10.1016/j.stress.2025.100751","DOIUrl":"10.1016/j.stress.2025.100751","url":null,"abstract":"<div><div>In this comprehensive review, we highlight the current state and analyze the global impact of drought stress on rice production, explore research progress achieved and offer insights through timely or comparative analyses where applicable. More than half of the world's rice area is affected by drought with Asia being the most severely impacted followed by Sub-Saharan Africa and Latin America. Under limiting freshwater resources and climate change, the traditional rice cultivation witnessed a shift towards water-efficient techniques. Further, integrating under-exploited but potentially valuable traits in modern cultivars, including root architecture, optimized canopy structure, tillering plasticity, and key physiological traits, could help minimize drought-induced damage. Furthermore, although high-throughput phenotyping (HTP) has shown promise for rice drought tolerance breeding, its large-scale deployment has remained limited, despite recent efforts to collect phenotypic data using various HTP platforms under controlled and field conditions. Breakthroughs in drought stress research, including genetic mapping and genomics studies have enabled the identification of important QTLs, genes, and superior alleles/haplotypes. A total of 55 mapping studies are available in rice, which have identified 1452 QTLs involving 263 major QTLs associated with diverse traits contributing to drought stress tolerance. In addition, around 305 cloned and/or characterized genes have been associated with drought-responsive traits in rice. Moreover, 81 important transgenes tested in rice have been shown to enhance drought resilience in transgenic rice plants. Additionally, direct selection for grain yield under drought and utilization of drought-yield QTLs in marker-assisted breeding programs, has resulted in the release of several drought-tolerant rice varieties across South and South–East Asia. Future research direction and the potential of emerging technologies to enhance drought tolerance in rice is emphasized. The holistic approach outlined guides future research and breeding endeavors, fostering sustainable and resilient rice production in the face of changing climatic conditions.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"15 ","pages":"Article 100751"},"PeriodicalIF":6.8,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143098871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanistic study on the mitigation of cadmium accumulation in Ligusticum sinense cv. Chuanxiong through plant growth-promoting rhizobacteria Arthrobacter sp. CX-2","authors":"Shu-qi Niu ,&nbsp;Ting Li ,&nbsp;Lin Liu ,&nbsp;Xiu-wen Bao ,&nbsp;Xing-mao Yang ,&nbsp;Hao-ran Song ,&nbsp;Yang Li ,&nbsp;Jing Bai ,&nbsp;Li-ying He ,&nbsp;Qing-he Wang ,&nbsp;Si-jing Liu ,&nbsp;Jin-lin Guo","doi":"10.1016/j.stress.2025.100748","DOIUrl":"10.1016/j.stress.2025.100748","url":null,"abstract":"<div><div><em>Ligusticum sinense</em> cv. Chuanxiong is commonly used in traditional Chinese medicine (TCM) to promote blood circulation, dispel wind, and relieve pain. However, the accumulation of cadmium (Cd) in <em>L. Chuanxiong</em> and Cd pollution in its genuine producing areas have resulted in significant concerns about excessive Cd levels in these medicinal materials. In this study, <em>Arthrobacter</em> sp. CX-2, a plant growth-promoting rhizobacteria (PGPR) strain from <em>L. Chuanxiong</em> rhizosphere soil, could promote growth and decrease Cd accumulation of <em>L. Chuanxiong</em> in the field. Inoculation CX-2 significantly increased the dry weight of rhizome (36.98 %), meanwhile decreased the Cd content in leaves (20.50 %) and rhizomes (33.23 %). Specifically, CX-2 could markedly reduce Cd transportation capacity of <em>L. Chuanxiong</em>, such as the translocation factor (TF) _Leaf/root (30.66 %) and TF _rhizome/root (41.66 %). Above all, as a TCM, the pharmacological effects of <em>L. Chuanxiong</em> were not affected by the addition of CX-2, which was confirmed through cell experiments. For rhizosphere soil, CX-2 significantly decreased the exchangeable Cd content by 36.84 % and enhanced the availability of nutrients. Metagenome analysis demonstrated that the structure, composition, and function of the rhizosphere microbial community significantly changed in the CX-2 group. There were 24 dominant differential microorganisms in the CK group and 29 in the CX-2 group. Among them, Nitrospirae, Acidobacteria, and Gemmatimonadaceae dominated in the CX-2 group. In addition, regulation of Carbohydrate metabolism, Energy metabolism, and Xenobiotics biodegradation and metabolism pathways by CX-2 in rhizosphere microorganisms might ultimately lead to a change in Cd accumulation of <em>L. Chuanxiong</em>. To our knowledge, this is the first study to explore the application of PGPR from the rhizosphere of <em>L. Chuanxiong</em> in the field environment, promoting its growth and reducing its Cd content. This work provides insights into using PGPR and offers a promising biotechnological approach to solve Cd accumulation in medical plants.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"15 ","pages":"Article 100748"},"PeriodicalIF":6.8,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143098947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic regulatory pathways of plant flowering time affected by abiotic stress","authors":"Jingru Wang ,&nbsp;Qi Wang ,&nbsp;Jing Gao ,&nbsp;Yi Lei ,&nbsp;Jie Zhang ,&nbsp;Jiaqi Zou ,&nbsp;Zhaohong Lu ,&nbsp;Siqi Li ,&nbsp;Na Lei ,&nbsp;Diwakar Dhungana ,&nbsp;Yuanlong Ma ,&nbsp;Xianming Tang ,&nbsp;Feng Yang ,&nbsp;Wenyu Yang","doi":"10.1016/j.stress.2025.100747","DOIUrl":"10.1016/j.stress.2025.100747","url":null,"abstract":"<div><div>The flowering of plants is a complex biological process that signifies the transition from vegetative growth to reproductive growth. Choosing the optimal timing for flowering is crucial for plant reproduction and yield. The timing of flowering in plants is influenced not only by their developmental status but also by external abiotic stress factors. This paper summarizes the effects of abiotic stress factors such as light, temperature, moisture, and nitrogen on flowering time, reviews the genes involved in the regulation of flowering time by environmental factors, and outlines the corresponding genetic regulatory pathways. By analyzing these abiotic stress factors and related genes, this study reveals the multilayered regulatory network through which plants alter their flowering time, aiming to provide insights into the molecular mechanisms underlying the adaptability of flowering time.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"15 ","pages":"Article 100747"},"PeriodicalIF":6.8,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143149366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fennel and other aqueous Apiaceae byproduct extracts as novel bioprotectants: Unravelling their bioactivity, working mechanism and bioactive compounds","authors":"Karen De Kock ,&nbsp;Pierfrancesco Motti ,&nbsp;Eva Degroote ,&nbsp;Maaike Perneel ,&nbsp;Bart Van Droogenbroeck ,&nbsp;Bruno P.A. Cammue ,&nbsp;Monica Höfte ,&nbsp;Kristof Demeestere ,&nbsp;Sven Mangelinckx ,&nbsp;Danny Geelen ,&nbsp;Tina Kyndt","doi":"10.1016/j.stress.2025.100750","DOIUrl":"10.1016/j.stress.2025.100750","url":null,"abstract":"<div><div>With regard to circular economy, upcycling organic waste streams to products with a biopesticidal activity represents a promising approach in integrated pest management. The current study demonstrates the induced resistance (IR) activity of seven aqueous extracts from byproducts of the <em>Apiaceae</em> plant family in rice to the root-knot nematode <em>Meloidogyne graminicola</em>. Furthermore, we unveiled that the systemic IR activity of an aqueous extract from fennel (FWE) is conserved among monocot and dicot plants and is effective against a wide range of plant pathogens as it induces resistance in rice against <em>Pyricularia oryzae</em>, in sugar beet against <em>Heterodera schachtiii</em>, in tomato against <em>Meloidogyne incognita</em> and in Arabidopsis against <em>Hyaloperonospora arabidopsidis</em> and <em>Botrytis cinerea.</em> The extract also showed a strong direct nematicidal activity against <em>M. graminicola</em>, fungicidal activity against <em>P. oryzae</em> and bactericidal activity against <em>Pseudomonas syringae</em> pv. <em>tomato</em> DC3000. Transcriptome analysis in combination with complementary biochemical validation in FWE-treated rice plants underlined the importance of an altered reactive oxygen species (ROS) metabolism, lignin accumulation and elevated levels of abscisic acid (ABA),indole-3-acetic acid (IAA) and jasmonic acid (JA) in FWE-treated rice plants. Lastly, bioactivity-guided fractionation and identification revealed several small organic acids such as alanine, malic acid and <em>У</em>-aminobutyric acid (GABA) as potential IR eliciting constituents in the extracts. To summarize, our data demonstrates high potential for valorization of <em>Apiaceae</em> byproduct extracts as preventive or curative plant bioprotectants in near-future eco-friendly agricultural practices.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"15 ","pages":"Article 100750"},"PeriodicalIF":6.8,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143098869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Applications of rhamnolipid biosurfactants in agriculture","authors":"Elise Pierre ,&nbsp;Elodie Shaw ,&nbsp;Brian Corr ,&nbsp;Karine Pageau ,&nbsp;Sonia Rippa","doi":"10.1016/j.stress.2025.100749","DOIUrl":"10.1016/j.stress.2025.100749","url":null,"abstract":"<div><div>Rhamnolipids are glycolipid biosurfactants naturally produced by <em>Pseudomonas</em> and <em>Burkholderia</em> bacteria, well-known for their surface-active properties and eco-friendly advantages. Indeed, these compounds efficiently reduce the surface and interface tensions, possess potent biological activities against pathogens, and display low toxicity. Rhamnolipids thus hold significant promise for various industrial applications, including agriculture, which is in need of a transition to greener and more sustainable practices by reducing synthetic inputs. This review describes the knowledge about the antimicrobial properties of rhamnolipids against different phytopathogens, mainly fungal species, as well as their ability to trigger plant defense mechanisms and phytoprotection efficacy in different species. The recent literature investigating rhamnolipids as potential plant biostimulation agents, thanks to their abilities to improve soil health and plant tolerance to abiotic stresses, is also addressed. Finally, the prospect of rhamnolipids as a biopesticide and their overall contribution to sustainable agricultural practices is discussed.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"15 ","pages":"Article 100749"},"PeriodicalIF":6.8,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143098266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"LcTRB1, a telomere repeat binding protein, enhances litchi seed development under low temperature by triggering the transcription of LcASHR1","authors":"Zhi-Chan Liao ,&nbsp;Dan-Rong Xie ,&nbsp;Alam Intikhab ,&nbsp;Fang Qiao ,&nbsp;Farhat Abbas ,&nbsp;Ren-Fang Zeng ,&nbsp;Xu-Ming Huang ,&nbsp;Hui-Cong Wang","doi":"10.1016/j.stress.2025.100745","DOIUrl":"10.1016/j.stress.2025.100745","url":null,"abstract":"<div><div>The seed development is a crucial step in ensuring a healthy fruit set, and the ultimate seed size is a significant quality feature in fruit crops. Litchi seed development is a fascinating process because of its remarkable plasticity. Previous study has indicated that the partial seed abortion phenotype of litchi cv. ‘Guiwei’ is induced by thermo-sensitive sterility and self-sterility. The comprehension of how genetic background and temperature influence the litchi seed development is limited. Here, we target <em>LcASHR1</em>, a putative histone-lysine N-methyltransferase gene, and <em>LcEMF2</em>, an EMBRYONIC FLOWER 2 gene, that were shown to be more highly expressed in the large seed cultivar ‘Huaizhi’ than in the small seed cultivar ‘Guiwei’. In addition, they were found to be enhanced in response to low temperatures, a condition flavoring the seed development of ‘Guiwei’ seeds. Among them, only the knockdown of <em>LcASHR1</em> in litchi resulted in tiny seeds and a higher level of seed abortion rate. Conversely, overexpression of <em>LcASHR1</em> in <em>Arabidopsis</em> lines led to the production of larger seeds. Furthermore, LcTRB1, a putative telomere repeats binding protein, was identified as a upstream transact factor of <em>LcASHR1</em> by binding to the telo-boxes in the promoter. <em>LcTRB1</em> expression pattern largely corresponded to litchi seed size. Consistent with previous findings on <em>LcASHR1</em>, it has been observed that <em>LcTRB</em>1 positively affects seed development in both litchi and arabidopsis. Overall, our results indicated that LcTRB1 is linked with litchi seed development probably by modulating the expression of <em>LcASHR1</em>.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"15 ","pages":"Article 100745"},"PeriodicalIF":6.8,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143098392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trehalose mitigates sodium chloride toxicity by improving ion homeostasis, membrane stability, and antioxidant defense system in Indian mustard","authors":"Shaistul Islam ,&nbsp;Firoz Mohammad ,&nbsp;M. Nasir Khan ,&nbsp;Francisco J Corpas","doi":"10.1016/j.stress.2025.100743","DOIUrl":"10.1016/j.stress.2025.100743","url":null,"abstract":"<div><div>Salt stress, particularly sodium chloride (NaCl), poses a significant threat to agricultural sustainability by limiting crop growth and development. The present study aimed to examine the effect of leaf-applied trehalose (Tre) on ion homeostasis, photosynthesis, antioxidant defense mechanisms, osmolyte accumulation, cell viability, oxidative stress biomarkers, and histo-microscopical analyses in Indian mustard under NaCl stress. Sodium chloride stress drastically decreased growth, water status, protein content and net photosynthetic rate, while increasing lipid peroxidation, ion toxicity, cell death, and electrolyte leakage due to the excessive accumulation of reactive oxygen species (ROS) in Indian mustard. Foliar fertigation of Tre significantly increased growth traits, nutrient acquisition, chlorophyll content, osmolyte accumulation, protein content, antioxidant enzyme activities, stomatal aperture, and cell viability, while considerably reducing leaf Na⁺ content and NaCl-generated ROS toxicity in Indian mustard. The Tre application enhanced shoot dry weight by 16 %, net photosynthetic rate by 19.37 %, superoxide dismutase activity by 10 %, nitrogen content by 13.32 %, seed yield by 23 % and oil yield by 27 %, while decreased superoxide anion content by 26 %, sodium ion content by 14.47 % and malondialdehyde content by 11.41 % over 0 mM NaCl treatment. Moreover, the trehalose-mediated reduction in ROS production was further validated with histochemical and microscopical localization analyses. Our data support the notion that Tre supplementation improves NaCl stress tolerance in Indian mustard by mitigating the detrimental impacts of ionic and oxidative stress. This finding suggests that Tre could serve as an alternative biotechnological tool in other types of crops.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"15 ","pages":"Article 100743"},"PeriodicalIF":6.8,"publicationDate":"2025-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143148865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}