Plant Stress最新文献_第4页

OsGLP3-7 negatively regulates drought tolerance by suppressing amino acid, lipid, and jasmonic acid metabolic pathways in rice OsGLP3-7通过抑制水稻氨基酸、脂质和茉莉酸代谢途径负调控抗旱性

IF 6.8 Q1 PLANT SCIENCES

Plant Stress

Pub Date : 2025-12-16 DOI: 10.1016/j.stress.2025.101188

Yamei Ma , Liqun Jiang , Yongjie Jiang , Lingli Lu , Jiao Xue , Bingrui Sun , Hang Yu , Xingxue Mao , Shuwei Lv , Pingli Chen , Chen Li , Jing Zhang , Qing Liu

Although germin-like protein (GLP) family members have been demonstrated to play important roles in regulating plant biotic and abiotic stresses, their specific roles in regulating rice drought stress response are still largely unknown. In this study, we identified that the transcription of OsGLP3–7 was significantly down-regulated by PEG, cold and NaCl stress treatments. Knockout of OsGLP3–7 enhanced plant tolerance whereas overexpression of OsGLP3–7 decreased plant tolerance to PEG6000-mimicked drought stress and water-deficient drought stress, as manifested by the survival rates, water loss rates and ion leakages compared to the wild-type plants. Transcriptomic analysis showed that the expression levels of genes involved in jasmonic acid (JA) metabolism, amino acid metabolism and lipid metabolism were remarkably lower in the OsGLP3–7 knockout plants but higher in the OsGLP3–7 overexpressing plants relative to the wild-type plants under stress treatment. Correspondingly, metabolome analysis revealed that the OsGLP3–7 knockout plants had lower JA, amino acids and lipids contents, whereas the OsGLP3–7 overexpressing plants had higher JA, amino acids and lipids contents than wild-type plants. Exogenous methyl jasmonate (MeJA) treatment reduced the drought tolerance of OsGLP3–7 knockout plants. These findings suggest that OsGLP3–7 negatively regulates rice drought tolerance by inactivating the JA, amino acid and lipids metabolic pathways, holding a promise target for improving drought tolerance through gene editing approaches.

虽然发芽样蛋白（GLP）家族成员已被证明在调节植物生物和非生物胁迫中发挥重要作用，但它们在调节水稻干旱胁迫反应中的具体作用仍不清楚。在本研究中，我们发现OsGLP3-7的转录在PEG、低温和NaCl胁迫处理下显著下调。敲除OsGLP3-7增强了植物对peg6000模拟干旱胁迫和缺水干旱胁迫的耐受性，而过表达OsGLP3-7则降低了植物对peg6000模拟干旱胁迫和缺水干旱胁迫的耐受性，表现在与野生型植物相比的存活率、失水率和离子泄漏上。转录组学分析显示，胁迫处理下OsGLP3-7敲除植株茉莉酸代谢、氨基酸代谢和脂质代谢相关基因的表达水平显著低于野生型植株，而OsGLP3-7过表达植株茉莉酸代谢、氨基酸代谢和脂质代谢相关基因的表达水平显著高于野生型植株。代谢组学分析显示，OsGLP3-7基因敲除植株的JA、氨基酸和脂质含量较低，而OsGLP3-7过表达植株的JA、氨基酸和脂质含量高于野生型植株。外源茉莉酸甲酯（MeJA）处理降低了OsGLP3-7基因敲除植株的耐旱性。这些发现表明，OsGLP3-7通过失活JA、氨基酸和脂质代谢途径负调控水稻抗旱性，是通过基因编辑方法提高水稻抗旱性的一个有希望的靶点。

{"title":"OsGLP3-7 negatively regulates drought tolerance by suppressing amino acid, lipid, and jasmonic acid metabolic pathways in rice","authors":"Yamei Ma , Liqun Jiang , Yongjie Jiang , Lingli Lu , Jiao Xue , Bingrui Sun , Hang Yu , Xingxue Mao , Shuwei Lv , Pingli Chen , Chen Li , Jing Zhang , Qing Liu","doi":"10.1016/j.stress.2025.101188","DOIUrl":"10.1016/j.stress.2025.101188","url":null,"abstract":"<div><div>Although germin-like protein (GLP) family members have been demonstrated to play important roles in regulating plant biotic and abiotic stresses, their specific roles in regulating rice drought stress response are still largely unknown. In this study, we identified that the transcription of <em>OsGLP3–7</em> was significantly down-regulated by PEG, cold and NaCl stress treatments. Knockout of <em>OsGLP3–7</em> enhanced plant tolerance whereas overexpression of <em>OsGLP3–7</em> decreased plant tolerance to PEG6000-mimicked drought stress and water-deficient drought stress, as manifested by the survival rates, water loss rates and ion leakages compared to the wild-type plants. Transcriptomic analysis showed that the expression levels of genes involved in jasmonic acid (JA) metabolism, amino acid metabolism and lipid metabolism were remarkably lower in the <em>OsGLP3–7</em> knockout plants but higher in the <em>OsGLP3–7</em> overexpressing plants relative to the wild-type plants under stress treatment. Correspondingly, metabolome analysis revealed that the <em>OsGLP3–7</em> knockout plants had lower JA, amino acids and lipids contents, whereas the <em>OsGLP3–7</em> overexpressing plants had higher JA, amino acids and lipids contents than wild-type plants. Exogenous methyl jasmonate (MeJA) treatment reduced the drought tolerance of <em>OsGLP3–7</em> knockout plants. These findings suggest that <em>OsGLP3–7</em> negatively regulates rice drought tolerance by inactivating the JA, amino acid and lipids metabolic pathways, holding a promise target for improving drought tolerance through gene editing approaches.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"19 ","pages":"Article 101188"},"PeriodicalIF":6.8,"publicationDate":"2025-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145790342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional analysis identifies the novel kinase activity of rice OsTLP16.5 in the thylakoid lumen 功能分析鉴定了水稻类囊体管中OsTLP16.5的新激酶活性

IF 6.8 Q1 PLANT SCIENCES

Plant Stress

Pub Date : 2025-12-13 DOI: 10.1016/j.stress.2025.101185

Zhenhui Kang, Linglong Deng, Yali Wang, Qiong Ren, Jiao Zhong, Yu Duan, Tong Qin

Protein phosphorylation is considered to facilitate the PSII repair cycle. The kinases and phosphatases responsible for the modification of PSII core and LHCII subunits are well understood, however, knowledge is limited about the role of phosphorylation in the thylakoid lumen. The acid phosphatase AtTLP18.3 has been characterized for over a decade, but the kinase(s) still remains unidentified. Indeed, the processes of disassembly and reassembly of the OEC represent a largely unexplored area within the PSII repair cycle. Our findings establish OsTLP16.5, the rice ortholog of MPH2, as the first functionally validated lumenal kinase. OsTLP16.5 is able to phosphorylate OsPsbP1 in vitro and physically interact with it in vivo. Phosphorylation of OsPsbP1 appears to directly influence the dissociation of the OEC from the PSII core monomer, thereby providing an explanation for why loss of MPH2 interferes with the proper disassembly of PSII monomers throughout the PSII repair. Our results support a model in which phosphorylation of lumenal OsPsbP1 by OsTLP16.5 facilitates PSII repair, thereby maintaining photosynthetic efficiency and agronomic performance in rice.

蛋白质磷酸化被认为促进PSII修复周期。目前对PSII核心和LHCII亚基修饰的激酶和磷酸酶已经了解得很清楚，然而，关于磷酸化在类囊体管腔中的作用的知识有限。酸性磷酸酶AtTLP18.3已经被鉴定了十多年，但激酶(s)仍然是未知的。事实上，在PSII维修周期中，OEC的拆卸和重组过程在很大程度上是一个未开发的领域。我们的研究结果证实，水稻中与MPH2同源的OsTLP16.5是第一个功能性验证的管状激酶。OsTLP16.5在体外能够磷酸化OsPsbP1，并在体内与OsPsbP1相互作用。OsPsbP1的磷酸化似乎直接影响OEC与PSII核心单体的解离，从而解释了为什么在PSII修复过程中，MPH2的缺失会干扰PSII单体的适当分解。我们的研究结果支持了一个模型，即OsTLP16.5对管腔OsPsbP1的磷酸化促进了PSII的修复，从而维持了水稻的光合效率和农艺性能。

{"title":"Functional analysis identifies the novel kinase activity of rice OsTLP16.5 in the thylakoid lumen","authors":"Zhenhui Kang, Linglong Deng, Yali Wang, Qiong Ren, Jiao Zhong, Yu Duan, Tong Qin","doi":"10.1016/j.stress.2025.101185","DOIUrl":"10.1016/j.stress.2025.101185","url":null,"abstract":"<div><div>Protein phosphorylation is considered to facilitate the PSII repair cycle. The kinases and phosphatases responsible for the modification of PSII core and LHCII subunits are well understood, however, knowledge is limited about the role of phosphorylation in the thylakoid lumen. The acid phosphatase AtTLP18.3 has been characterized for over a decade, but the kinase(s) still remains unidentified. Indeed, the processes of disassembly and reassembly of the OEC represent a largely unexplored area within the PSII repair cycle. Our findings establish OsTLP16.5, the rice ortholog of MPH2, as the first functionally validated lumenal kinase. OsTLP16.5 is able to phosphorylate OsPsbP1 <em>in vitro</em> and physically interact with it <em>in vivo</em>. Phosphorylation of OsPsbP1 appears to directly influence the dissociation of the OEC from the PSII core monomer, thereby providing an explanation for why loss of MPH2 interferes with the proper disassembly of PSII monomers throughout the PSII repair. Our results support a model in which phosphorylation of lumenal OsPsbP1 by OsTLP16.5 facilitates PSII repair, thereby maintaining photosynthetic efficiency and agronomic performance in rice.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"19 ","pages":"Article 101185"},"PeriodicalIF":6.8,"publicationDate":"2025-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145790339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

AcGSTU1 enhances salt tolerance of polyploid Actinidia chinensis by dosage effect and is regulated by transcription factor AcERF3 AcGSTU1通过剂量效应增强多倍体猕猴桃耐盐性，并受转录因子AcERF3调控

IF 6.8 Q1 PLANT SCIENCES

Plant Stress

Pub Date : 2025-12-12 DOI: 10.1016/j.stress.2025.101181

Ying Wu , Yunzhi Lin , Liu Wang , Yingzhen Wang , Zhiwen Ma , Meng Zhao , Yuyu Huo , Tao Zhang , Cheng Zhang , Lihuan Wang , Yongsheng Liu , Pengpeng Zheng

Polyploidy is a dominant feature of plant diversity and plays an important role in the speciation and evolution of plant species. Compared to diploids, polyploids exhibit greater adaptability to a broader spectrum of environmental conditions. However, the physiological and molecular mechanisms of adaptation remain largely unexplored. In this study, transcriptome, genetic and biochemical techniques were used to investigate the molecular mechanisms of salt stress resistance between diploid and polyploidy (tetraploid, and hexaploid) Actinidia chinensis. The analysis of differently expressed genes (DEGs) reveals significant differences in gene expression among Actinidia chinensis leaves of different ploidy, displaying distinct dose-response characteristics. Further analysis obtained a gene with significant positive dosage effect, namely AcGSTU1. Overexpressed the AcGSTU1 in both Arabidopsis thaliana and Actinidia chinensis revealed that AcGSTU1 was responsible for polyploidy Actinidia chinensis tolerance to salt stress. In addition, AcGSTU1 may enhance the salt stress tolerance of Actinidia chinensis by increasing ROS scavenging. Besides, we found that transcription factor AcERF3 positively regulates salt stress tolerance by directly binding on the promoter of AcGSTU1 to activate its expression. In conclusion, our work provides insights into the adaptation mechanism of polyploid Actinidia chinensis to salt stress.

多倍体是植物多样性的显性特征，在植物物种形成和进化中起着重要作用。与二倍体相比，多倍体对更广泛的环境条件表现出更强的适应性。然而，适应的生理和分子机制在很大程度上仍未被探索。利用转录组、遗传和生化等技术，研究了猕猴桃二倍体和多倍体（四倍体和六倍体）抗盐胁迫的分子机制。不同倍性猕猴桃叶片基因表达差异分析显示，不同倍性猕猴桃叶片基因表达差异显著，表现出不同的剂量效应特征。进一步分析得到一个具有显著正剂量效应的基因，即AcGSTU1。AcGSTU1在拟南芥和猕猴桃中均过表达，表明AcGSTU1与猕猴桃多倍体耐盐胁迫有关。此外，AcGSTU1可能通过增强活性氧清除能力来增强猕猴桃的耐盐性。此外，我们发现转录因子AcERF3通过直接结合AcGSTU1启动子激活其表达，正向调节盐胁迫耐受性。本研究揭示了猕猴桃多倍体对盐胁迫的适应机制。

{"title":"AcGSTU1 enhances salt tolerance of polyploid Actinidia chinensis by dosage effect and is regulated by transcription factor AcERF3","authors":"Ying Wu , Yunzhi Lin , Liu Wang , Yingzhen Wang , Zhiwen Ma , Meng Zhao , Yuyu Huo , Tao Zhang , Cheng Zhang , Lihuan Wang , Yongsheng Liu , Pengpeng Zheng","doi":"10.1016/j.stress.2025.101181","DOIUrl":"10.1016/j.stress.2025.101181","url":null,"abstract":"<div><div>Polyploidy is a dominant feature of plant diversity and plays an important role in the speciation and evolution of plant species. Compared to diploids, polyploids exhibit greater adaptability to a broader spectrum of environmental conditions. However, the physiological and molecular mechanisms of adaptation remain largely unexplored. In this study, transcriptome, genetic and biochemical techniques were used to investigate the molecular mechanisms of salt stress resistance between diploid and polyploidy (tetraploid, and hexaploid) <em>Actinidia chinensis</em>. The analysis of differently expressed genes (DEGs) reveals significant differences in gene expression among <em>Actinidia chinensis</em> leaves of different ploidy, displaying distinct dose-response characteristics. Further analysis obtained a gene with significant positive dosage effect, namely <em>AcGSTU1</em>. Overexpressed the <em>AcGSTU1</em> in both <em>Arabidopsis thaliana</em> and <em>Actinidia chinensis</em> revealed that <em>AcGSTU1</em> was responsible for polyploidy <em>Actinidia chinensis</em> tolerance to salt stress. In addition, AcGSTU1 may enhance the salt stress tolerance of <em>Actinidia chinensis</em> by increasing ROS scavenging. Besides, we found that transcription factor AcERF3 positively regulates salt stress tolerance by directly binding on the promoter of <em>AcGSTU1</em> to activate its expression. In conclusion, our work provides insights into the adaptation mechanism of polyploid <em>Actinidia chinensis</em> to salt stress.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"19 ","pages":"Article 101181"},"PeriodicalIF":6.8,"publicationDate":"2025-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145790340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing San Marzano dwarf tomato performance: The role of biochar under saline irrigation conditions 盐水灌溉条件下生物炭对圣马扎诺矮番茄生产性能的提高作用

IF 6.8 Q1 PLANT SCIENCES

Plant Stress

Pub Date : 2025-12-11 DOI: 10.1016/j.stress.2025.101184

Matteo Lentini , Michele Ciriello , Petronia Carillo , Giovanna Marta Fusco , Rosalinda Nicastro , Antonio Pannico , Francesco Primo Vaccari , Youssef Rouphael , Stefania De Pascale

A greenhouse experiment was conducted in an unheated greenhouse to study the growth, physiology, metabolic mechanisms and yield of tomato in response to salinity stress with the addition of biochar. It was hypothesized that biochar supplementation could enhance plant growth and yield by mitigating the deleterious effect of salinity from NaCl due to its inherent sorption capacity. Tomato plants were exposed to three concentrations of NaCl (0, 40 and 80 mM) and three different percentages of biochar (0, 1 and 2 % v/v). Biochar supplementation improved the physiological response of salinized plants both at the vegetative stage (increased leaf fresh weight by 41.53 % at moderate salinity levels) and at the end of the experiment due to a significant reduction in the uptake of toxic Na⁺ (-35 %) and Cl^- (-41 %) ions. In addition, biochar application positively influenced the balance of sugars, starch and amino acids, supporting a coordinated metabolic response to salt stress. There were significant increases in metabolites such as proline and γ-Aminobutyric-acid (GABA), contributing to stress adaptation and improved fruit quality. The findings underscore the value of biochar's potential as a sustainable solution for amending soils and improving tomato production under adverse conditions such as NaCl stress.

在不加热的温室中，研究了添加生物炭对盐胁迫下番茄生长、生理、代谢机制和产量的影响。我们推测，生物炭的添加可以通过其固有的吸附能力来减轻NaCl对盐的有害影响，从而促进植物的生长和产量。番茄植株分别处理了3种浓度的NaCl（0、40和80 mM）和3种不同比例的生物炭（0、1和2% v/v）。添加生物炭改善了盐渍化植物在营养阶段（在中等盐度水平下叶片鲜重增加41.53%）和试验结束时的生理反应，这是由于生物炭显著减少了有毒Na+(- 35%)和Cl-(- 41%)离子的吸收。此外，施用生物炭对糖、淀粉和氨基酸的平衡产生积极影响，支持对盐胁迫的协调代谢反应。脯氨酸和γ-氨基丁酸（γ-氨基丁酸，GABA）等代谢物显著增加，有利于适应逆境，提高果实品质。这些发现强调了生物炭作为一种可持续解决方案的价值，它可以在NaCl胁迫等不利条件下改善土壤和提高番茄产量。

{"title":"Enhancing San Marzano dwarf tomato performance: The role of biochar under saline irrigation conditions","authors":"Matteo Lentini , Michele Ciriello , Petronia Carillo , Giovanna Marta Fusco , Rosalinda Nicastro , Antonio Pannico , Francesco Primo Vaccari , Youssef Rouphael , Stefania De Pascale","doi":"10.1016/j.stress.2025.101184","DOIUrl":"10.1016/j.stress.2025.101184","url":null,"abstract":"<div><div>A greenhouse experiment was conducted in an unheated greenhouse to study the growth, physiology, metabolic mechanisms and yield of tomato in response to salinity stress with the addition of biochar. It was hypothesized that biochar supplementation could enhance plant growth and yield by mitigating the deleterious effect of salinity from NaCl due to its inherent sorption capacity. Tomato plants were exposed to three concentrations of NaCl (0, 40 and 80 mM) and three different percentages of biochar (0, 1 and 2 % v/v). Biochar supplementation improved the physiological response of salinized plants both at the vegetative stage (increased leaf fresh weight by 41.53 % at moderate salinity levels) and at the end of the experiment due to a significant reduction in the uptake of toxic Na<sup>+</sup> (-35 %) and Cl<sup>-</sup> (-41 %) ions. In addition, biochar application positively influenced the balance of sugars, starch and amino acids, supporting a coordinated metabolic response to salt stress. There were significant increases in metabolites such as proline and γ-Aminobutyric-acid (GABA), contributing to stress adaptation and improved fruit quality. The findings underscore the value of biochar's potential as a sustainable solution for amending soils and improving tomato production under adverse conditions such as NaCl stress.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"19 ","pages":"Article 101184"},"PeriodicalIF":6.8,"publicationDate":"2025-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145790343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Boron alleviates fluoride stress in tea plant (Camellia sinensis) by targeting ion homeostasis and metabolic reprogramming 硼通过调控离子稳态和代谢重编程缓解茶树氟胁迫

IF 6.8 Q1 PLANT SCIENCES

Plant Stress

Pub Date : 2025-12-11 DOI: 10.1016/j.stress.2025.101183

Wenluan Xu, Jiasheng Huang, Yuxuan Jia, Yifei Fan, Mingle Wang, Hua Zhao, Dejiang Ni, Yuqiong Chen

The tea plant (Camellia sinensis) is a typical fluoride (F)-accumulating and F-tolerant species, but excessive F still induces stress, ultimately threatening safe tea production and human health. Given boron's (B) well-documented role in enhancing stress tolerance and maintaining ion homeostasis, this study aimed to investigate its unreported potential to reduce F accumulation in tea plant. We investigated two genotypes (the low-F cultivar FDDB and the high-F cultivar JGY) by integrating phenotypic, physiological, ionomic, gene expression, and metabolomic analyses. The results showed that B alleviated F-induced stress through multiple pathways: reducing F bioavailability via rhizosphere regulation, enhancing antioxidant defense systems, maintaining ion homeostasis through a B-Ca-Al-Mn-F centered regulatory network, and inducing genotype- and leaf age-specific metabolic reprogramming. Moreover, JGY showed stronger proactive regulation, and 10 μM B was optimal. These findings uncover B's novel role in alleviating F stress and highlight its potential as a practical agronomic strategy for reducing F accumulation in tea plant.

茶树（Camellia sinensis）是典型的氟(F)积累和耐氟物种，但过量的F仍然会产生应激，最终威胁到茶叶的安全生产和人体健康。鉴于硼(B)在增强胁迫耐受性和维持离子稳态方面的作用，本研究旨在探讨其在茶树中减少F积累的未报道的潜力。通过综合表型、生理、基因组学、基因表达和代谢组学分析，研究了两个基因型（低f品种FDDB和高f品种JGY）。结果表明，B通过多种途径缓解F诱导的胁迫：通过根际调控降低F的生物利用度，增强抗氧化防御系统，通过以B- ca - al - mn -F为中心的调控网络维持离子稳态，诱导基因型和叶龄特异性代谢重编程。JGY具有较强的主动调节作用，以10 μ B为最优。这些发现揭示了B在缓解F胁迫中的新作用，并突出了其作为减少茶树F积累的实用农艺策略的潜力。

{"title":"Boron alleviates fluoride stress in tea plant (Camellia sinensis) by targeting ion homeostasis and metabolic reprogramming","authors":"Wenluan Xu, Jiasheng Huang, Yuxuan Jia, Yifei Fan, Mingle Wang, Hua Zhao, Dejiang Ni, Yuqiong Chen","doi":"10.1016/j.stress.2025.101183","DOIUrl":"10.1016/j.stress.2025.101183","url":null,"abstract":"<div><div>The tea plant (<em>Camellia sinensis</em>) is a typical fluoride (F)-accumulating and F-tolerant species, but excessive F still induces stress, ultimately threatening safe tea production and human health. Given boron's (B) well-documented role in enhancing stress tolerance and maintaining ion homeostasis, this study aimed to investigate its unreported potential to reduce F accumulation in tea plant. We investigated two genotypes (the low-F cultivar FDDB and the high-F cultivar JGY) by integrating phenotypic, physiological, ionomic, gene expression, and metabolomic analyses. The results showed that B alleviated F-induced stress through multiple pathways: reducing F bioavailability via rhizosphere regulation, enhancing antioxidant defense systems, maintaining ion homeostasis through a B-Ca-Al-Mn-F centered regulatory network, and inducing genotype- and leaf age-specific metabolic reprogramming. Moreover, JGY showed stronger proactive regulation, and 10 μM B was optimal. These findings uncover B's novel role in alleviating F stress and highlight its potential as a practical agronomic strategy for reducing F accumulation in tea plant.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"19 ","pages":"Article 101183"},"PeriodicalIF":6.8,"publicationDate":"2025-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145790341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional roles of arabidopsis elongin A and C in UV stress tolerance and development 拟南芥长素A和C在紫外胁迫耐受和发育中的功能作用

IF 6.8 Q1 PLANT SCIENCES

Plant Stress

Pub Date : 2025-12-11 DOI: 10.1016/j.stress.2025.101180

Linda Alrayes , Ashly Bell , Jake Stout , Dana Schroeder

Elongins A (ELOA), B (ELOB), and C (ELOC) were originally identified in mammals as transcription elongation complex. Beyond this role, the three mammalian elongins function in E3 ligase complex that degrades the large subunit of the stalled RNA polymerase II after DNA damage. S. cerevisiae lacks ELOB but posseses ELOA and ELOC homologs which form a similar E3 ligase complex that performs a comparable function in targeting stalled RNA polymerase II for degradation. These yeast homologs are functionally conserved with their mammalian counterparts, as yeast ELOC enhances mammalian ELOA transcript elongation via interacting with it. However, yeast ELOA/C complex alone does not promote yeast transcriptional elongation. Their role in RNA polymerase II polyubiquitylation and degradation following DNA damage has been well characterized. In this study, we identified Arabidopsis elongin A homologue (ELOA; At2g42780) and examined the roles of ELOA and elongin C homologue (ELOC; At5g59140) in UV tolerance and development using molecular and genetic analyses. YFP-ELOA localized to nucleus and this pattern was not affected after UV treatment. However, YFP-ELOC localized to both nuclei and cytosol with reduced cytoplasmic signal post-UV exposure. Both Ateloa-2 and Ateloc-3 mutants exhibited enhanced UV-sensitive phenotypes in seedlings and adults. ELOA overexpression enhanced hypocotyl UV tolerance. Yeast two-hybrid assay confirmed the interaction between Arabidopsis ELOA and ELOC. Interestingly, ELOA and ELOC overexpression increased silique length and seed number, and ELOC overexpression also increased plant height. These findings demonstrate that Arabidopsis ELOA and ELOC homologs are required for UV tolerance and contribute to plant developmental regulation.

长链蛋白A （ELOA）、B （ELOB）和C （ELOC）最初在哺乳动物中被鉴定为转录延伸复合体。除了这个作用之外，这三种哺乳动物的长链蛋白还在E3连接酶复合体中发挥作用，该复合体在DNA损伤后降解停滞的RNA聚合酶II的大亚基。葡萄球菌缺乏ELOB，但具有ELOA和ELOC同源物，它们形成类似的E3连接酶复合物，在靶向停滞的RNA聚合酶II进行降解方面具有类似的功能。这些酵母同源物与哺乳动物同源物在功能上是保守的，因为酵母ELOC通过相互作用增强哺乳动物ELOA转录物的延伸。然而，酵母菌ELOA/C复合体单独不促进酵母菌的转录延伸。它们在RNA聚合酶II多泛素化和DNA损伤后降解中的作用已经得到了很好的表征。在这项研究中，我们鉴定了拟南芥长链蛋白A同源物（ELOA; At2g42780），并通过分子和遗传分析研究了ELOA和长链蛋白C同源物（ELOC; At5g59140）在紫外线耐受性和发育中的作用。YFP-ELOA定位于细胞核，紫外线处理后这种模式不受影响。然而，紫外线照射后，YFP-ELOC定位于细胞核和细胞质，胞质信号减少。Ateloa-2和Ateloc-3突变体在幼苗和成虫中均表现出增强的紫外线敏感表型。ELOA过表达增强了下胚轴对紫外线的耐受性。酵母双杂交实验证实了拟南芥ELOA与ELOC的互作。有趣的是，ELOA和ELOC过表达增加了单株长度和种子数量，ELOC过表达也增加了株高。这些发现表明拟南芥的ELOA和ELOC同源物是植物耐紫外线的必需物质，并参与植物的发育调控。

{"title":"Functional roles of arabidopsis elongin A and C in UV stress tolerance and development","authors":"Linda Alrayes , Ashly Bell , Jake Stout , Dana Schroeder","doi":"10.1016/j.stress.2025.101180","DOIUrl":"10.1016/j.stress.2025.101180","url":null,"abstract":"<div><div>Elongins A (ELOA), B (ELOB), and C (ELOC) were originally identified in mammals as transcription elongation complex. Beyond this role, the three mammalian elongins function in E3 ligase complex that degrades the large subunit of the stalled RNA polymerase II after DNA damage. <em>S. cerevisiae</em> lacks ELOB but posseses ELOA and ELOC homologs which form a similar E3 ligase complex that performs a comparable function in targeting stalled RNA polymerase II for degradation. These yeast homologs are functionally conserved with their mammalian counterparts, as yeast ELOC enhances mammalian ELOA transcript elongation via interacting with it. However, yeast ELOA/C complex alone does not promote yeast transcriptional elongation. Their role in RNA polymerase II polyubiquitylation and degradation following DNA damage has been well characterized. In this study, we identified <em>Arabidopsis</em> elongin A homologue (ELOA; At2g42780) and examined the roles of ELOA and elongin C homologue (ELOC; At5g59140) in UV tolerance and development using molecular and genetic analyses. YFP-ELOA localized to nucleus and this pattern was not affected after UV treatment. However, YFP-ELOC localized to both nuclei and cytosol with reduced cytoplasmic signal post-UV exposure. Both <em>Ateloa-2</em> and <em>Ateloc-3</em> mutants exhibited enhanced UV-sensitive phenotypes in seedlings and adults. ELOA overexpression enhanced hypocotyl UV tolerance. Yeast two-hybrid assay confirmed the interaction between <em>Arabidopsis</em> ELOA and ELOC. Interestingly, ELOA and ELOC overexpression increased silique length and seed number, and ELOC overexpression also increased plant height. These findings demonstrate that <em>Arabidopsis</em> ELOA and ELOC homologs are required for UV tolerance and contribute to plant developmental regulation.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"19 ","pages":"Article 101180"},"PeriodicalIF":6.8,"publicationDate":"2025-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145790427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proteomic and physiological insights into drought mitigation by exogenously applied calcium nanoparticles in Brassica napus 外源应用钙纳米颗粒对甘蓝型油菜抗旱的蛋白质组学和生理学研究

IF 6.8 Q1 PLANT SCIENCES

Plant Stress

Pub Date : 2025-12-10 DOI: 10.1016/j.stress.2025.101179

Ahsan Ayyaz , Iram Batool , Mingchao Xu , Ying Li , Tongjun Qin , Fakhir Hannan , Muhammad Ahsan Farooq , Weijun Zhou , Yanhui Wang , Ling Xu

Drought stress poses a significant threat to rapeseed (Brassica napus) productivity. This study investigates the efficacy of calcium nanoparticles (Ca-NPs) in mitigating drought stress, with a specific focus on the proteomic reprogramming that underlies this resilience. Physiological assessments confirmed that Ca-NP application restored plant growth and biomass under drought conditions, correlating with enhanced photosynthetic efficiency and a potentiated antioxidant response. A pivotal and novel finding, revealed through non-invasive micro-test technology (NMT), was that Ca-NPs prevented drought-induced pathological leakage of cellular Ca²⁺, thereby stabilizing membrane integrity and suggesting a primed state for stress signaling. Quantitative proteomic analysis (TMT-based) identified significant alterations in the abundance of key proteins involved in central metabolic processes. Specifically, Ca-NPs upregulated proteins associated with carbon fixation, porphyrin and chlorophyll metabolism, and the glutathione-mediated antioxidant pathway, providing the first comprehensive proteomic evidence of a reconfiguration of primary and defensive metabolism in rapeseed under Ca-NP treatment. Furthermore, weighted protein co-expression network analysis (WGCNA) identified highly connected hub proteins within modules strongly correlated with improved physiological traits, pinpointing critical functional components of the drought response. These findings demonstrate that Ca-NPs do not merely alleviate symptoms but actively prime rapeseed plants by enforcing ionic homeostasis and orchestrating a protective proteomic landscape, positioning them as a powerful nano-priming strategy for sustainable agriculture. Moreover, exogenously applied Ca-NPs can be further used for rapeseed seed coating. The coated seeds are then applied in the precision drill-seeding of rapeseed, jointly promoting the increase in large-scale per-unit yield of rapeseed.

干旱胁迫对油菜籽（Brassica napus）的产量构成了重大威胁。本研究探讨了钙纳米颗粒（Ca-NPs）在缓解干旱胁迫方面的功效，并特别关注了这种抗旱性背后的蛋白质组重编程。生理评估证实，Ca-NP的施用恢复了干旱条件下植物的生长和生物量，这与提高光合效率和增强抗氧化反应有关。通过非侵入性微测试技术（NMT）揭示的一项关键的新发现是，Ca- nps阻止了干旱诱导的细胞Ca 2 +病理渗漏，从而稳定了膜的完整性，并提示了胁迫信号的启动状态。定量蛋白质组学分析（基于tmt）发现了参与中枢代谢过程的关键蛋白丰度的显著变化。具体来说，Ca-NP上调了与碳固定、卟啉和叶绿素代谢以及谷胱甘肽介导的抗氧化途径相关的蛋白质，为Ca-NP处理下油菜籽初级和防御性代谢的重构提供了第一个全面的蛋白质组学证据。此外，加权蛋白共表达网络分析（WGCNA）发现了与改善生理性状密切相关的模块中高度连接的枢纽蛋白，从而精确定位了干旱响应的关键功能成分。这些发现表明，Ca-NPs不仅可以缓解症状，还可以通过加强离子稳态和协调保护性蛋白质组学景观来积极启动油菜籽植物，将其定位为可持续农业的强大纳米启动策略。此外，外源Ca-NPs可以进一步用于油菜籽包衣。将包衣种子应用于油菜籽精密钻播，共同促进油菜籽大规模单产的提高。

{"title":"Proteomic and physiological insights into drought mitigation by exogenously applied calcium nanoparticles in Brassica napus","authors":"Ahsan Ayyaz , Iram Batool , Mingchao Xu , Ying Li , Tongjun Qin , Fakhir Hannan , Muhammad Ahsan Farooq , Weijun Zhou , Yanhui Wang , Ling Xu","doi":"10.1016/j.stress.2025.101179","DOIUrl":"10.1016/j.stress.2025.101179","url":null,"abstract":"<div><div>Drought stress poses a significant threat to rapeseed (<em>Brassica napus</em>) productivity. This study investigates the efficacy of calcium nanoparticles (Ca-NPs) in mitigating drought stress, with a specific focus on the proteomic reprogramming that underlies this resilience. Physiological assessments confirmed that Ca-NP application restored plant growth and biomass under drought conditions, correlating with enhanced photosynthetic efficiency and a potentiated antioxidant response. A pivotal and novel finding, revealed through non-invasive micro-test technology (NMT), was that Ca-NPs prevented drought-induced pathological leakage of cellular Ca²⁺, thereby stabilizing membrane integrity and suggesting a primed state for stress signaling. Quantitative proteomic analysis (TMT-based) identified significant alterations in the abundance of key proteins involved in central metabolic processes. Specifically, Ca-NPs upregulated proteins associated with carbon fixation, porphyrin and chlorophyll metabolism, and the glutathione-mediated antioxidant pathway, providing the first comprehensive proteomic evidence of a reconfiguration of primary and defensive metabolism in rapeseed under Ca-NP treatment. Furthermore, weighted protein co-expression network analysis (WGCNA) identified highly connected hub proteins within modules strongly correlated with improved physiological traits, pinpointing critical functional components of the drought response. These findings demonstrate that Ca-NPs do not merely alleviate symptoms but actively prime rapeseed plants by enforcing ionic homeostasis and orchestrating a protective proteomic landscape, positioning them as a powerful nano-priming strategy for sustainable agriculture. Moreover, exogenously applied Ca-NPs can be further used for rapeseed seed coating. The coated seeds are then applied in the precision drill-seeding of rapeseed, jointly promoting the increase in large-scale per-unit yield of rapeseed.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"19 ","pages":"Article 101179"},"PeriodicalIF":6.8,"publicationDate":"2025-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145790345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bibliometric and systematic review of biostimulant research under drought stress 干旱胁迫下生物刺激素研究的文献计量学和系统综述

IF 6.8 Q1 PLANT SCIENCES

Plant Stress

Pub Date : 2025-12-09 DOI: 10.1016/j.stress.2025.101177

Waqas Liaqat , Muhammad Tanveer Altaf , Muhammad Faheem Jan , Asia Maqbool , Awais Ali , Celaleddin Barutçular , Faheem Shehzad Baloch , Mehdi Rahimi

Drought stress, a critical environmental factor impacting global crop productivity, is exacerbated by the challenges of climate change. Biostimulants (BS), substances that enhance plant resilience to abiotic stress without directly providing nutrients, have emerged as a promising solution for mitigating drought induced damage. However, the increasing volume and diversity of research in this area necessitate a systematic approach to evaluate its development. This advanced bibliometric and systematic review analyzes 499 publications from the Web of Science Core Collection published between 2015 and 2024. The review focuses on the role of BS in drought stress management and identifies key research trends, influential authors, major journals. Emerging topics include phytohormonal regulation, antioxidant defense, and microbial interactions. The results reveal a substantial increase in research output, reflecting a growing global interest in BS as an eco-friendly alternative to conventional fertilizers. The review highlights several primary mechanisms by which BS enhance drought tolerance., These mechanisms include the modulation of plant stress response pathways and antioxidant systems. Additionally, microbial-based BS play a role in improving soil health and nutrient uptake. Despite notable progress, several gaps remain. These include the need for standardized evaluation methods and deeper molecular insights into BS action. This review offers a comprehensive overview of the field and provides insights for future research directions. It emphasizes the potential of BS to contribute to a more holistic approach to sustainable agriculture under climate change.

干旱胁迫是影响全球作物生产力的一个重要环境因素，气候变化的挑战加剧了干旱胁迫。生物刺激剂（BS）是一种增强植物对非生物胁迫的适应能力而不直接提供营养的物质，已成为减轻干旱引起的损害的一种有希望的解决方案。然而，这一领域研究的数量和多样性不断增加，需要一种系统的方法来评估其发展。这项先进的文献计量学和系统综述分析了2015年至2024年间出版的Web of Science核心馆藏的499份出版物。本文综述了BS在干旱胁迫管理中的作用，并确定了主要的研究趋势、有影响力的作者和主要期刊。新兴主题包括植物激素调节、抗氧化防御和微生物相互作用。结果显示，研究产出大幅增加，反映出全球对BS作为传统肥料的环保替代品的兴趣日益浓厚。这篇综述强调了几个主要的机制，其中BS提高抗旱性。这些机制包括植物胁迫反应途径和抗氧化系统的调节。此外，基于微生物的BS在改善土壤健康和养分吸收方面发挥作用。尽管取得了显著进展，但仍存在一些差距。其中包括需要标准化的评估方法和对BS作用的更深入的分子见解。本文对该领域进行了全面的综述，并对未来的研究方向进行了展望。它强调了BS在气候变化下为更全面的可持续农业方法做出贡献的潜力。

{"title":"Bibliometric and systematic review of biostimulant research under drought stress","authors":"Waqas Liaqat , Muhammad Tanveer Altaf , Muhammad Faheem Jan , Asia Maqbool , Awais Ali , Celaleddin Barutçular , Faheem Shehzad Baloch , Mehdi Rahimi","doi":"10.1016/j.stress.2025.101177","DOIUrl":"10.1016/j.stress.2025.101177","url":null,"abstract":"<div><div>Drought stress, a critical environmental factor impacting global crop productivity, is exacerbated by the challenges of climate change. Biostimulants (BS), substances that enhance plant resilience to abiotic stress without directly providing nutrients, have emerged as a promising solution for mitigating drought induced damage. However, the increasing volume and diversity of research in this area necessitate a systematic approach to evaluate its development. This advanced bibliometric and systematic review analyzes 499 publications from the Web of Science Core Collection published between 2015 and 2024. The review focuses on the role of BS in drought stress management and identifies key research trends, influential authors, major journals. Emerging topics include phytohormonal regulation, antioxidant defense, and microbial interactions. The results reveal a substantial increase in research output, reflecting a growing global interest in BS as an eco-friendly alternative to conventional fertilizers. The review highlights several primary mechanisms by which BS enhance drought tolerance., These mechanisms include the modulation of plant stress response pathways and antioxidant systems. Additionally, microbial-based BS play a role in improving soil health and nutrient uptake. Despite notable progress, several gaps remain. These include the need for standardized evaluation methods and deeper molecular insights into BS action. This review offers a comprehensive overview of the field and provides insights for future research directions. It emphasizes the potential of BS to contribute to a more holistic approach to sustainable agriculture under climate change.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"19 ","pages":"Article 101177"},"PeriodicalIF":6.8,"publicationDate":"2025-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145790336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phytomelatonin and secondary metabolites: Operative strategies to induce plant abiotic stress tolerance 褪黑素和次生代谢物：诱导植物非生物胁迫耐受性的手术策略

IF 6.8 Q1 PLANT SCIENCES

Plant Stress

Pub Date : 2025-12-09 DOI: 10.1016/j.stress.2025.101178

Sayeda Khatoon , Chirag Maheshwari , Sarika Kumari , Moksh Mahajan , Harmanjit Kaur , Anis Ahmad Chaudhary , Hamdi Bendif , M․ Iqbal R․ Khan

Abiotic stresses, such as salt, drought, heavy metals and extreme temperatures, severely affect plant growth and metabolism, leading to reduced crop productivity. In response to these challenges, plants accelerate the biosynthesis of secondary metabolites (SMs), including polyphenols, flavonoids, terpenoids and glucosinolates, which act as defense molecules to mitigate abiotic stress. Additionally, SMs not only contribute to stress tolerance but also have significant applications in pharmaceuticals, food industries and agriculture. Phytomelatonin (MT), a ubiquitously present indoleamine, has emerged as a key regulator of SMs production to stimulate defense mechanisms. For instance, MT could facilitate polyamines (PAs) accumulation, including putrescine (Put), spermidine (Spd) and spermine (Spm) by 3.1, 3.5, and 2.1 folds, respectively, under waterlogging stress in alfalfa (Medicago sativa), suggesting the potential of MT to modulate SMs for improving stress tolerance. However, there is lack of a comprehensive review highlighting the significance of how MT could modulate SMs under abiotic stress. Thus, in the present review, we aimed to discuss the potential of MT to activate SMs production and promote abiotic stress tolerance, along with shedding significance on the future implications of omic(s)-integrated gene editing strategies to generate tolerant crop lines.

非生物胁迫，如盐、干旱、重金属和极端温度，严重影响植物生长和代谢，导致作物产量下降。为了应对这些挑战，植物加速了次生代谢物（SMs）的生物合成，包括多酚、黄酮类、萜类和硫代葡萄糖苷，它们作为防御分子来缓解非生物胁迫。此外，SMs不仅有助于抗逆性，而且在制药，食品工业和农业中也有重要的应用。褪黑激素（MT）是一种普遍存在的吲哚胺，已成为SMs产生的关键调节因子，以刺激防御机制。例如，MT对紫花苜蓿（Medicago sativa）涝渍胁迫下腐胺（Put）、亚精胺（Spd）和精胺（Spm）等多胺（PAs）积累的促进作用分别为3.1倍、3.5倍和2.1倍，表明MT可能通过调节sm来提高抗逆性。然而，缺乏一个全面的综述，强调在非生物胁迫下MT如何调节SMs的意义。因此，在本综述中，我们旨在讨论MT激活SMs生产和促进非生物胁迫耐受性的潜力，以及对未来基因组(s)整合基因编辑策略产生耐受性作物品系的意义。

{"title":"Phytomelatonin and secondary metabolites: Operative strategies to induce plant abiotic stress tolerance","authors":"Sayeda Khatoon , Chirag Maheshwari , Sarika Kumari , Moksh Mahajan , Harmanjit Kaur , Anis Ahmad Chaudhary , Hamdi Bendif , M․ Iqbal R․ Khan","doi":"10.1016/j.stress.2025.101178","DOIUrl":"10.1016/j.stress.2025.101178","url":null,"abstract":"<div><div>Abiotic stresses, such as salt, drought, heavy metals and extreme temperatures, severely affect plant growth and metabolism, leading to reduced crop productivity. In response to these challenges, plants accelerate the biosynthesis of secondary metabolites (SMs), including polyphenols, flavonoids, terpenoids and glucosinolates, which act as defense molecules to mitigate abiotic stress. Additionally, SMs not only contribute to stress tolerance but also have significant applications in pharmaceuticals, food industries and agriculture. Phytomelatonin (MT), a ubiquitously present indoleamine, has emerged as a key regulator of SMs production to stimulate defense mechanisms. For instance, MT could facilitate polyamines (PAs) accumulation, including putrescine (Put), spermidine (Spd) and spermine (Spm) by 3.1, 3.5, and 2.1 folds, respectively, under waterlogging stress in alfalfa (<em>Medicago sativa</em>), suggesting the potential of MT to modulate SMs for improving stress tolerance. However, there is lack of a comprehensive review highlighting the significance of how MT could modulate SMs under abiotic stress. Thus, in the present review, we aimed to discuss the potential of MT to activate SMs production and promote abiotic stress tolerance, along with shedding significance on the future implications of omic(s)-integrated gene editing strategies to generate tolerant crop lines.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"19 ","pages":"Article 101178"},"PeriodicalIF":6.8,"publicationDate":"2025-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145790337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sex-specific responses in photosystem regulation, antioxidant defence, and energy metabolic adaptations in mulberry (Morus alba L.) to waterlogging stress 桑树（Morus alba L.）对涝渍胁迫光系统调节、抗氧化防御和能量代谢适应的性别特异性响应

IF 6.8 Q1 PLANT SCIENCES

Plant Stress

Pub Date : 2025-12-08 DOI: 10.1016/j.stress.2025.101176

Xue Deng , Yaseen Khan , Muhammad-Faheem Jan , Ling-Feng Miao , Fan Yang

Waterlogging stress is an increasingly common abiotic stress caused by climate change, significantly impairing physiological and metabolic processes, reducing growth and overall productivity. Previous studies have examined the physiological response of mulberry (Morus alba L.) to waterlogging stress; however, sex-specific differences and underlying adaptive mechanisms remain underexplored. This study examines the sex-specific adaptive mechanisms of mulberry to waterlogging, focusing on photosystem regulation, antioxidant defence, and energy metabolism. The results showed that female plants exhibited greater waterlogging tolerance through a multifaceted strategy, whereas males showed systemic dysregulation in photosynthesis, antioxidant, and metabolic processes. The gas exchange analyses revealed that females optimized CO₂ assimilation through moderate stomatal regulation, maintaining higher intercellular CO₂ concentration. Chlorophyll fluorescence indicated higher non-photochemical quenching (NPQ) and stable Fv/Fm, preserving photosystem integrity. Furthermore, the proteomic profiling revealed upregulation of key photosynthetic proteins (e.g., PsbB, PsbQ, PsaN, PetH) in females, while males exhibited downregulation of photosystem components (PsbD, PsbH, PsaA/B). In antioxidant defence, females activated leucine aminopeptidase 3 and glutathione S-transferase, efficiently scavenging reactive oxygen species (ROS), while males showed impaired ROS management. In energy metabolism, females sustained ATP production under hypoxia through enhanced glycolysis and TCA cycle activity, while males showed metabolic disruptions. These findings indicate that female plants achieve superior stress tolerance through integrated regulation of photosystems, ROS homeostasis, and metabolic flexibility, offering insights for breeding waterlogging-resilient cultivars and contributing to ecological restoration in flood-prone ecosystems.

涝渍胁迫是一种日益普遍的由气候变化引起的非生物胁迫，显著损害生理和代谢过程，降低生长和整体生产力。以往的研究考察了桑树（Morus alba L.）对涝渍胁迫的生理反应；然而，性别特异性差异和潜在的适应机制仍未得到充分探讨。本研究探讨了桑树对涝渍的性别特异性适应机制，重点关注光系统调节、抗氧化防御和能量代谢。结果表明，雌性植物通过多方面的策略表现出更强的耐涝能力，而雄性植物在光合作用、抗氧化和代谢过程中表现出系统性的失调。气体交换分析表明，雌性通过适度的气孔调节来优化CO2同化，保持较高的细胞间CO2浓度。叶绿素荧光显示较高的非光化学猝灭（NPQ）和稳定的Fv/Fm，保持了光系统的完整性。此外，蛋白质组学分析显示，雌性的关键光合蛋白（PsbB、PsbQ、PsaN、PetH）上调，而雄性的光合系统成分（PsbD、PsbH、PsaA/B）下调。在抗氧化防御中，雌性激活亮氨酸氨基肽酶3和谷胱甘肽s -转移酶，有效清除活性氧（ROS），而雄性则表现出活性氧管理受损。在能量代谢方面，雌性在缺氧条件下通过增强糖酵解和TCA循环活性来维持ATP的产生，而雄性则表现出代谢中断。这些发现表明，雌性植物通过光系统、活性氧稳态和代谢灵活性的综合调控实现了优越的抗逆性，为培育抗涝品种和促进洪水易发生态系统的生态恢复提供了新的思路。

{"title":"Sex-specific responses in photosystem regulation, antioxidant defence, and energy metabolic adaptations in mulberry (Morus alba L.) to waterlogging stress","authors":"Xue Deng , Yaseen Khan , Muhammad-Faheem Jan , Ling-Feng Miao , Fan Yang","doi":"10.1016/j.stress.2025.101176","DOIUrl":"10.1016/j.stress.2025.101176","url":null,"abstract":"<div><div>Waterlogging stress is an increasingly common abiotic stress caused by climate change, significantly impairing physiological and metabolic processes, reducing growth and overall productivity. Previous studies have examined the physiological response of mulberry (<em>Morus alba</em> L.) to waterlogging stress; however, sex-specific differences and underlying adaptive mechanisms remain underexplored. This study examines the sex-specific adaptive mechanisms of mulberry to waterlogging, focusing on photosystem regulation, antioxidant defence, and energy metabolism. The results showed that female plants exhibited greater waterlogging tolerance through a multifaceted strategy, whereas males showed systemic dysregulation in photosynthesis, antioxidant, and metabolic processes. The gas exchange analyses revealed that females optimized CO<sub>2</sub> assimilation through moderate stomatal regulation, maintaining higher intercellular CO<sub>2</sub> concentration. Chlorophyll fluorescence indicated higher non-photochemical quenching (NPQ) and stable Fv/Fm, preserving photosystem integrity. Furthermore, the proteomic profiling revealed upregulation of key photosynthetic proteins (e.g., PsbB, PsbQ, PsaN, PetH) in females, while males exhibited downregulation of photosystem components (PsbD, PsbH, PsaA/B). In antioxidant defence, females activated leucine aminopeptidase 3 and glutathione S-transferase, efficiently scavenging reactive oxygen species (ROS), while males showed impaired ROS management. In energy metabolism, females sustained ATP production under hypoxia through enhanced glycolysis and TCA cycle activity, while males showed metabolic disruptions. These findings indicate that female plants achieve superior stress tolerance through integrated regulation of photosystems, ROS homeostasis, and metabolic flexibility, offering insights for breeding waterlogging-resilient cultivars and contributing to ecological restoration in flood-prone ecosystems.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"19 ","pages":"Article 101176"},"PeriodicalIF":6.8,"publicationDate":"2025-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145737357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0