Plant Stress最新文献

{"title":"Phenolic compounds-enriched extract recovered from two-phase olive pomace serves as plant immunostimulants and broad-spectrum antimicrobials against phytopathogens including Xylella fastidiosa","authors":"Marco Greco ,&nbsp;María Fuertes-Rabanal ,&nbsp;Carlos Frey ,&nbsp;Carmine Del Grosso ,&nbsp;Daniele Coculo ,&nbsp;Pasquale Moretti ,&nbsp;Pasquale Saldarelli ,&nbsp;Savino Agresti ,&nbsp;Rosanna Caliandro ,&nbsp;Hugo Mélida ,&nbsp;Vincenzo Lionetti","doi":"10.1016/j.stress.2024.100655","DOIUrl":"10.1016/j.stress.2024.100655","url":null,"abstract":"<div><div>The production of extra virgin olive oil generates significant amounts of olive mill waste, whose disposal leads to severe environmental impacts, especially due to the high content of phenolic compounds. In this study, a pomace phenolic extract composed of hydroxytyrosol, tyrosol, verbascoside, and oleuropein was obtained from the liquid fraction of two-phase olive pomace and explored for its antimicrobial properties and potential as plant immunostimulants. The olive pomace extract exhibited a broad range of antimicrobial activity against important phytopathogens, including the bacteria <em>Xylella fastidiosa, Pseudomonas syringae</em>, and <em>Pectobacterium carotovorum</em>, as well as the fungi <em>Colletotrichum graminicola, Fusarium graminearum</em>, and <em>Botrytis cinerea</em>. The extract induced key features of plant innate immunity in Arabidopsis seedlings, including hydrogen peroxide production, phosphorylation of mitogen-activated protein kinase MAPK6, and upregulation of defence genes, such as <em>CYP81F2, FRK1</em>, and <em>WRKY53</em>, suggesting the activation of early signalling cascades leading to the production of indole glucosinolates and salicylic acid. The immune activation pathways induced by the phenolic extract did not always match those triggered by well-known oligogalacturonide elicitors. Notably, pretreatment of adult Arabidopsis and tomato plants with the phenolic compounds-enriched extract primed responses and enhanced their resistance against <em>B. cinerea</em> and <em>P. syringae</em>. Our findings demonstrate the potential to upcycle two-phase olive pomace into plant protectants, offering a promising alternative to reduce reliance on chemically synthesized pesticides in integrated pest management programs.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"14 ","pages":"Article 100655"},"PeriodicalIF":6.8,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142554821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Water and nutrient availability modulate the salinity stress response in Olea europaea cv. Arbequina","authors":"Marzia Vergine ,&nbsp;Emily Rose Palm ,&nbsp;Anna Maria Salzano ,&nbsp;Carmine Negro ,&nbsp;Werther Guidi Nissim ,&nbsp;Leonardo Sabbatini ,&nbsp;Raffaella Balestrini ,&nbsp;Maria Concetta de Pinto ,&nbsp;Nunzio Dipierro ,&nbsp;Gholamreza Gohari ,&nbsp;Vasileios Fotopoulos ,&nbsp;Stefano Mancuso ,&nbsp;Andrea Luvisi ,&nbsp;Luigi De Bellis ,&nbsp;Andrea Scaloni ,&nbsp;Federico Vita","doi":"10.1016/j.stress.2024.100648","DOIUrl":"10.1016/j.stress.2024.100648","url":null,"abstract":"<div><div>Salinity stress represents a key factor for global agriculture. Plants can respond to salinity stress by adapting their physiology in different ways with the aim of limiting reductions in growth and development. Importantly, moisture retention capacity, permeability and nutrient availability of substrates represent critical variables for plants as they may further influence the effect of osmotic stress. Here, a multidisciplinary approach was applied to evaluate the role of two different substrates, peat and perlite, on 2-year-old potted cuttings of <em>Olea europaea</em> (cultivar Arbequina) under different salinity stress conditions (0, 100 and 200 mM NaCl). Biometric and physiological data indicate that plants potted in perlite (AP) generally present lower growth and photosynthetic rates when compared with peat (AS) in combination with salinity stress. Ion measurements indicate a rise in Na⁺ accumulation with increasing stress severity, which alters the ion ratio in both substrates. In addition, differences occurred in polyphenol contents, with a general increase in quinic acid and rutin contents in AS and AP samples, respectively. Metabolomic and biometric data were also coupled with metabarcoding analysis, which indicates that the moderate salinity treatment (100 mM NaCl, T100) reshaped the endophytic community of plants grown on both substrates. Taken together, the data suggest that the strategy used by a glycophytic species such as the olive tree to cope with salinity stress seems to be highly related to availability of water and nutrients. The lack of both may be simulated by perlite, enhancing the effect of salinity stress response in woody plants. Lastly, applying the beneficial endophytic bacterial taxa identified here could represent a step forward in increasing plant defence and nutrient uptake and reducing inputs for modern and more sustainable agriculture.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"14 ","pages":"Article 100648"},"PeriodicalIF":6.8,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142554822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative analysis of the NF-Y transcription factor family identifies VaNF-YA6 as a positive regulator of salt and drought tolerance in grapevine","authors":"Shixiong Lu,&nbsp;Xueting Zhou,&nbsp;Xu Huang,&nbsp;Baozhen Zeng,&nbsp;Huimin Gou,&nbsp;Weifeng Ma,&nbsp;Zonghuan Ma,&nbsp;Juan Mao,&nbsp;Baihong Chen","doi":"10.1016/j.stress.2024.100658","DOIUrl":"10.1016/j.stress.2024.100658","url":null,"abstract":"<div><div><em>Nuclear factor Y</em> transcription factors (<em>NF-Y</em> TFs) play crucial roles in plant responses to abiotic stress. However, there is a lack of research on the comparative analysis of evolutionary relationships, real-time quantitative fluorescence PCR (RT-qPCR), and functions of <em>NF-Y</em> TFs to screen key <em>NF-Y</em> TFs that are resistant to salt and drought stresses between <em>Vitis vinifera</em> (<em>V. vinifera</em>) and <em>Vitis amurensis</em> (<em>V. amurensis</em>). In this study, 27 and 26 <em>NF-Y</em> TFs were identified in <em>V. vinifera</em> and <em>V. amurensis</em>, respectively, and were divided into three subgroups. Subcellular localization prediction revealed that <em>NF-Ys</em> TFs were mainly located in the nucleus. Interestingly, the NF-YA protein sequence of ‘NTKKLDWEFWGCCDDCEKWFGGCC’ was lost in the <em>V. vinifera</em> compared to <em>V. amurensis</em>, whereas the sequence ‘SSVYSQPWWGHSIVCVA’ was gained, thus, these sequences might be closely related to the functions performed. RT-qPCR analysis of ‘Pinot Noir’ (cultivated variety) and ‘Zuoyouhong’ (wild variety) plantlets demonstrated that the expression levels of <em>VaNF-YA6, VaNF-YB5, VvNF-YA3, VvNF-YA5</em>, and <em>VvNF-YC2</em> were significantly upregulated under 400 mmol·L^-1 NaCl and 10% PEG treatments for 24 h Subcellular localization showed that the VaNF-YA6-GFP fusion protein was functioned primarily in the nucleus. Overexpression of <em>VaNF-YA6</em> in grapevine leaves and <em>Arabidopsis thaliana</em> (<em>Arabidopsis</em>) could significantly enhance tolerance to salt and drought stresses by improving <em>VvSOS2, VvSOS3, VvABF3, VvCPK6</em> expression levels, enzyme activities, and other protective substances. In summary, our study provides a theoretical basis for the further use of <em>VaNF-YA6</em> to improve salt and drought resistance in grapevines.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"14 ","pages":"Article 100658"},"PeriodicalIF":6.8,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142554823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cannabis sativa genotypes with larger leaf areas have higher potential to adjust stomatal size and density in response to water deficit: The effect on stomatal conductance and physiological stomatal behaviour","authors":"Matthew Haworth ,&nbsp;Andrea Carli ,&nbsp;Vincenzo Montesano ,&nbsp;Dilek Killi ,&nbsp;André Fabbri ,&nbsp;Raffaella Balestrini ,&nbsp;Giovanni Marino ,&nbsp;Mauro Centritto","doi":"10.1016/j.stress.2024.100649","DOIUrl":"10.1016/j.stress.2024.100649","url":null,"abstract":"<div><div>Effective stomatal control is fundamental to successful plant responses to water deficit stress. Control of stomatal conductance (<em>G</em>_s) can be exerted through modification of stomatal morphology (size and density) in newly developing leaves, or physiological adjustment of stomatal pore aperture. We investigated the potential coordination of stomatal morphological and physiological responses to water deficit in three varieties of hemp (<em>Cannabis sativa</em> L.) grown under field conditions. The three hemp varieties had contrasting leaf areas under well-watered irrigation (control): Earlina 8FC &lt; Fedora 17 &lt; Fibror 79. Stomatal size, density and the speed of physiological adjustment of <em>G</em>_s were not significantly different under full irrigation. Under water deficit conditions the leaf areas of the two varieties with the largest leaves decreased to match those of Earlina 8FC. This reduction in leaf area, alongside an increase in stomatal initiation, resulted in higher densities of smaller stomata. This effect was most evident in Fibror 79 that showed the largest reduction in leaf area, increase in stomatal density and decrease in stomatal size. This corresponded to the most rapid physiological adjustment of <em>G</em>_s to fluctuations in photosynthetic photon flux density of the hemp varieties occurring in Fibror 79 when subject to water deficit. The coincidence of the fastest velocity of <em>G</em>_s adjustment with the highest densities of small stomata may support interpretations of a functional advantage of high densities of small stomata in the physiological regulation of <em>G</em>_s to fluctuating conditions. The larger leaf area of Fibror 79 appeared to be associated with higher capacity to respond to water deficit through modification of stomatal morphology and physiological behaviour. This result indicates that phenotyping of crop species and genotypes to identify traits conducive to water deficit tolerance through effective stomatal control should consider the foliar plasticity of genotypes to water deficit, and the potential implications for stomatal morphological and physiological control of transpirative water loss and photosynthetic CO₂-uptake.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"14 ","pages":"Article 100649"},"PeriodicalIF":6.8,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142573436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Zinc nano and zinc ethylenediaminetetraacetic acid (EDTA) mediated water deficit stress alleviation in pearl millet (Pennisetum glaucum (L.) R. Br.): Photosystem II electron transport and pigment dynamics","authors":"Arun K. Shanker ,&nbsp;V. Visha Kumari ,&nbsp;N. Jyothi Lakshmi ,&nbsp;M.S. Rao ,&nbsp;V. Girijaveni ,&nbsp;V.K. Singh ,&nbsp;M.R. Krupashankar ,&nbsp;Tarunendu Singh","doi":"10.1016/j.stress.2024.100651","DOIUrl":"10.1016/j.stress.2024.100651","url":null,"abstract":"<div><div>Water stress adversely affects the photosynthetic apparatus and pigment composition in plants, leading to reduced yields and compromised plant health. Zinc (Zn) foliar spray in nano form presents a potential solution to ameliorate water deficit stress. We attempt here a detailed dissection of electron transport in Photosystem II (PSII) through studies on chlorophyll a fast fluorescence kinetics and non-photochemical quenching (NPQ) and pigment dynamics in response to water stress. We also investigated the possible changes in these processes and the regulation of it by Zn Nano and Zn Ethylenediaminetetraacetic acid (EDTA) foliar sprays that may lead to amelioration of stress. Our results indicated that water stress created a \"traffic jam\" like situation in the electron transport system of Photosystem II, leading to decreased photosynthetic efficiency. Treatments with water deficit stress + Zn Nano (particle size &lt; 90 nm) spray with Zn concentration at 20 mg L⁻¹ and water deficit stress + Zn EDTA spray (solid material size ∼ 100 µm) with Zn concentration at 240 mg L⁻¹ effectively ameliorated water deficit stress by its action on flux ratio parameters viz., quantum yield for electron transport (φ_E0), probability of electron transport beyond Q_A (ψ₀) and quantum yield of electron transport from Q_A^⁻ to PS1 end electron acceptors (ϕ_R0) and also the specific fluxes and phenomenological fluxes. These treatments positively influenced chlorophyll content, and xanthophyll components, including violaxanthin, antheraxanthin, and zeaxanthin, and reduced NPQ and the de expoxidation state. Higher concentrations of Zn Nano foliar spray (water stress + Zn Nano spray Zn @ 30 mg L⁻¹) did not ameliorate water deficit stress as effectively as the lower concentrations, although this higher concentration was not in any way toxic. This lack of stress amelioration at higher concentrations of Zn Nano spray may be due to physiological limitations of elemental zinc action within the plant. Our findings suggest that Zn foliar sprays in Nano and EDTA form at optimum concentrations can significantly improve plant resilience to water stress.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"14 ","pages":"Article 100651"},"PeriodicalIF":6.8,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142593351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Further enhancement of cold tolerance in rice seedlings by Piriformospora indica collaborating with plant growth-promoting bacteria: Evidence from the antioxidant defense, osmoregulation, photosynthesis, and related genes","authors":"Feng Shi ,&nbsp;Siyu Zhu ,&nbsp;Honghe Li ,&nbsp;Bo Zhang ,&nbsp;Jie Liu ,&nbsp;Fuqiang Song","doi":"10.1016/j.stress.2024.100656","DOIUrl":"10.1016/j.stress.2024.100656","url":null,"abstract":"<div><div>As the global population continues to grow, so does the demand for food. However, cold stress due to global climate change greatly threatens the safe production of rice. <em>Piriformospora indica</em> plays a pivotal role as a functional microbiota in improving cold tolerance in rice. However, the addition of <em>P. indica</em> alone has a limited effect. In this study, an attempt was made to select plant growth-promoting bacteria (<em>Agrobacterium rhizogenes</em> and <em>Bacillus subtilis</em>) as helper bacteria to help <em>P. indica</em> function better and further enhance rice cold tolerance. Under cold stress, the co-addition of the three beneficial microorganisms significantly increased the biomass, photosynthetic performance indicators, osmoregulatory substance contents, and antioxidant enzyme activities of rice. <em>P. indica</em> and helper bacteria significantly reduced the cold stress-induced increases in malondialdehyde content and electrolyte leakage rate in rice leaves and triggered a substantial upregulation of cold-tolerant genes in rice. This suggests that <em>P. indica</em> and helper bacteria can synergistically improve the cold resistance of rice. The findings of the research offer a conceptual foundation and practical assistance for enhancing rice's resilience to cold temperatures.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"14 ","pages":"Article 100656"},"PeriodicalIF":6.8,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142585995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Early monitoring of drought stress in safflower (Carthamus tinctorius L.) using hyperspectral imaging: A comparison of machine learning tools and feature selection approaches","authors":"Fatemeh Salek ,&nbsp;Seyed Ahmad Mireei ,&nbsp;Abbas Hemmat ,&nbsp;Mehrnoosh Jafari ,&nbsp;Mohammad R. Sabzalian ,&nbsp;Majid Nazeri ,&nbsp;Wouter Saeys","doi":"10.1016/j.stress.2024.100653","DOIUrl":"10.1016/j.stress.2024.100653","url":null,"abstract":"<div><div>Early detection of drought stress is essential for preventing permanent plant damage and minimizing yield loss. This study utilized hyperspectral imaging at the leaf level to visualize drought stress in safflower plants (<em>Carthamus tinctorius</em> L.). Three safflower genotypes, Palenus, A82, and IL-111, were cultivated under three irrigation levels. Stress conditions were simulated by depleting 50%, 70%, and 90% of soil water content, representing unstressed (US), mild stress (MS), and severe stress (SS) conditions, respectively. Hyperspectral images of leaf samples were captured before any visible signs of water scarcity emerged. Classification analysis was performed using the full mean spectral data with partial least squares discriminant analysis, soft independent modeling of class analogy (SIMCA), support vector machines, and artificial neural network (ANN) classifiers. Feature selection methods were applied to extract the most informative wavebands, and ANN was used to build predictive models. Spatial analysis involved pixel-wise classification using both unsupervised (k-means clustering) and supervised (best classifiers) approaches. ANN outperformed other classifiers using the entire spectral data, effectively distinguishing US, MS, and SS classes in the Palenus, A82, and IL-111 genotypes, achieving F1-scores of 92.22%, 96.01%, and 96.47%, respectively. Among the feature selection methods, SIMCA-based features excelled in monitoring stress conditions in the Palenus and A82 genotypes. In supervised spatial analysis, ANN models clearly depicted the progression of stress in leaves across different genotypes. This study demonstrates the potential of hyperspectral imaging to differentiate various levels of drought stress in safflower, an important oilseed crop.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"14 ","pages":"Article 100653"},"PeriodicalIF":6.8,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142554826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrative mechanisms of plant salt tolerance: Biological pathways, phytohormonal regulation, and technological innovations","authors":"Abdul Waheed ,&nbsp;Lu Zhuo ,&nbsp;Minghui Wang ,&nbsp;Xu Hailiang ,&nbsp;Zewen Tong ,&nbsp;Cuhan Wang ,&nbsp;Aishajiang Aili","doi":"10.1016/j.stress.2024.100652","DOIUrl":"10.1016/j.stress.2024.100652","url":null,"abstract":"<div><div>Salt stress is a major environmental challenge that profoundly impacts plant growth and development. The ability of plants to cope with high salinity involves with a complex network of biological mechanisms including osmoregulation, redox and ionic homeostasis, and hormones or light signaling-mediated growth adjustments. These adaptive responses are governed by various functional components that interact to modulate plant stress tolerance. This review provides a comprehensive overview of the current understanding of these mechanisms, focusing on the intricate regulatory networks that underpin plant salt tolerance. We explore the processes involved in the perception of salt stress, where plants detect changes in osmotic and ionic conditions, and the subsequent signaling pathways that activate stress responses. Key phytohormones such as abscisic acid (ABA), ethylene (ET), and brassinosteroids (BRs) play pivotal roles in these processes by regulating gene expression and coordinating adaptive growth responses. Additionally, this review explores physiological mechanisms like ion homeostasis, compatible solute synthesis, and antioxidant defense, alongside the role of root microbiota in enhancing nutrient uptake and stress mitigation under salinity. Emerging nanobiotechnologies, including nano-fertilizers and stress-sensing technologies, are highlighted for their role in improving plant resilience. By integrating molecular biology, plant physiology, and advanced technologies, the review emphasizes the multidisciplinary strategies needed to develop salt-tolerant cultivars and optimize agricultural practices in saline environments.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"14 ","pages":"Article 100652"},"PeriodicalIF":6.8,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142554824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineering biotic stress tolerance via CRISPR-Cas mediated genome editing in crop plants","authors":"Amjad Hussain ,&nbsp;Mamoona Munir ,&nbsp;Awais Khalid ,&nbsp;Musrat Ali ,&nbsp;Mohammed Amanullah ,&nbsp;Qurban Ali ,&nbsp;Hakim Manghwar","doi":"10.1016/j.stress.2024.100650","DOIUrl":"10.1016/j.stress.2024.100650","url":null,"abstract":"<div><div>Plants are incessantly challenged by a plethora of plant pests and pathogens, putting global agricultural productivity and food security at stake. Over several decades, various strategies have been developed in agriculture to overcome plant diseases and insect pests. With chemical control that remains effective but involves severe ecological and environmental concerns, conventional and transgenic breeding strategies have been primarily deployed to generate new varieties with novel genetic mutations. Though these strategies present a pivotal role in plant development, in part, they normally include extensive and labor-intensive processes. CRISPR-Cas technology, a genome editing tool, has opened new avenues to accelerate plant breeding by creating disease and pest resistance in a wide range of plants. CRISPR-Cas revolutionized agriculture by limiting yield losses due to biotic stress and minimizing reliance on pesticide usage. Here, we summarize the advances of CRISPR-Cas technology and the applications of this technology in disease and pest resistance development in crop plants. In addition, the review also discusses the advantages and concerns of CRISPR-Cas genome editing in crop plants.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"14 ","pages":"Article 100650"},"PeriodicalIF":6.8,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142573438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rtlp2 enhances thermotolerance in rice","authors":"Wen Du ,&nbsp;Xiaohan Zhang ,&nbsp;Jian Zhang ,&nbsp;Yugui Wu ,&nbsp;Xiaojun Chen ,&nbsp;Qiuhong Chen ,&nbsp;Dongyang Lei","doi":"10.1016/j.stress.2024.100647","DOIUrl":"10.1016/j.stress.2024.100647","url":null,"abstract":"<div><div>The harmful effects of heat stress on agricultural production are becoming increasingly severe. Thaumatin-like proteins (TLPs) have been demonstrated to play crucial roles in regulating plant resistance to biotic and abiotic stresses. However, their involvement in plant thermotolerance is poorly understood. This study aims to investigate the major-effect gene in a key quantitative trait locus (QTL) associated with rice thermotolerance, namely <em>Rtlp2</em>, which encodes a rice TLP. The Rtlp2 protein was found to be localized in the endoplasmic reticulum (ER). Rice seedlings overexpressing the <em>Rtlp2</em> gene showed significantly enhanced thermotolerance compared to the wild type, while <em>rtlp2</em> mutant exhibited increased susceptibility to heat stress. After 48 h of heat stress treatment, rice plants overexpressing <em>Rtlp2</em> exhibited reduced accumulation of hydrogen peroxide (H₂O₂) and cell death compared to the wild-type. Conversely, the mutant plants showed the opposite behavior. The results of gene expression level analyses revealed that <em>Rtlp2</em> positively regulates rice thermotolerance through the modulation of rice heat shock transcription factors (HSFs) and heat shock proteins (HSPs) network. After undergoing natural heat stress in the field, the rice lines overexpressing <em>Rtlp2</em> showed higher seed setting rate and yield per plant compared to the wild-type. In addition, <em>Rtlp2</em> has also been found to positively regulate grain length and grain weight in rice. This study provides valuable resources for addressing the challenge of increasing environmental heat and promoting the breeding of heat-tolerant rice varieties.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"14 ","pages":"Article 100647"},"PeriodicalIF":6.8,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142539752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}