Euterpe edulis is a promising palm tree in the fruit industry, which produces a pulp similar to açaí. Here, we investigated a commercial population of E. edulis for fruit production and pulp commercialization, which has been the subject of breeding studies aimed at selecting matrices. In this study, we compared the genetic diversity of the commercial population in the municipality of Rio Novo do Sul - ES, compared with natural populations distributed in the Brazilian Atlantic Forest and Cerrado biomes, as well as to evaluate the phenotypic diversity for fruit traits over three years, in the commercial population. In the analysis of 637 individuals of E. edulis by genomic SNPs, 388 of the commercial population were compared with another 249 individuals from 26 natural populations of 26 locations. The analysis evaluated fruit traits in 2018, 2019, and 2021. The results indicate considerable allelic variability, relevant heterozygosity in the commercial population, and high diversity and low inbreeding. The natural populations are divided into three groups, and the populations south of the Atlantic Forest are distinguished from those analyzed. Among the 13 fruit traits evaluated, fruit and seed diameter showed the least variation with production traits. This study highlights that the managed area has a significant reserve of genetic variability, the potential to maintain the incentive for the sustainable use of the fruit for pulp production, and establishes a solid basis for practices that join the species conservation and sustainable management. Besides, the research highlighted the presence of exclusive genetic groups and alleles in the populations north and south of the Atlantic Forest, stressing the importance of conserving all populations to maintain this species’ genetic variability.
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