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2007 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems最新文献

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Growth Process of Novel Thin Films by Directed Self Organization of Nanoparticles 纳米颗粒定向自组织制备新型薄膜的研究
S. Promnimit, S. Pratontep, C. Thanachayanonr, J.K. Park, J. Dutta
This work reports on the growth process of multilayer thin films based on layer by layer (LBL) deposition of engineered nanoparticles. The LBL thin films were constructed with zinc sulphide nanoparticles capped with a polyelectrolyte such as chitosan, and gold nanoparticles stabilized by electrostatic charges. The LBL process of self assembly of nanoparticle monolayers & subsequent monoparticulate layered sandwiched units were built on silanized substrates by repeating the self-organization process. The authors discuss tie linear growth of the layers upon multiple dipping, the surface morphology of the obtained films, and the I-V characteristics with increasing film thicknesses.
本文报道了基于工程纳米颗粒逐层沉积的多层薄膜的生长过程。用壳聚糖等聚电解质包覆硫化锌纳米粒子和静电稳定的金纳米粒子制备了LBL薄膜。通过重复自组织过程,在硅化基底上构建了纳米颗粒单层自组装的LBL过程和随后的单层夹层单元。讨论了多次浸镀后膜层的线性生长、膜的表面形貌以及随膜厚增加的I-V特性。
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引用次数: 1
Study on the Cell Mechanics of MDCK Cells by Elastic Micro-pillars Arrays 弹性微柱阵列对MDCK细胞力学的研究
Chien-Wen Wang, Wei-Ren Chen, Ching-Chou Wu, Hsien-Chang Chang
The cellular force is one of the most important parameters of cellular physiological behavior and mechanical interactions between the cell and its environment which could regulate the cell migration, contractility and gene expression. In order to evidence the characteristics of the physiology and cellular mechanics of the epithelial cells, therefore, we use the micro-pillars arrays (MPAs) to measure the traction force of the Madin-Darby canine kidney (MDCK) cells. When MDCK cells adhere on the different geometry of MPAs, the cell will pull MPAs and cause the pillars displacement due to the traction force. Furthermore, the cellular image and the displacement of the pillars could be analyzed by optic microscope system, and could be evaluated the mechanical interactions between the cells and MPAs. Therefore, this method might help us understand the cellular mechanics and physiological phenomenon between the cells and underlying substrates.
细胞力是细胞生理行为和细胞与环境相互作用的重要参数之一,可以调节细胞的迁移、收缩和基因表达。因此,为了证明上皮细胞的生理和细胞力学特征,我们使用微柱阵列(MPAs)测量了Madin-Darby犬肾(MDCK)细胞的牵引力。当MDCK细胞粘附在不同几何形状的MPAs上时,细胞会对MPAs产生牵引力,使柱产生位移。此外,利用光学显微镜系统分析了细胞图像和柱的位移,并评价了细胞与MPAs之间的力学相互作用。因此,这种方法可能有助于我们了解细胞力学和细胞与底物之间的生理现象。
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引用次数: 4
Application of Conductometric Capillary Electrophoresis Microchip in Detect Organic and Inorganic Ions 毛细管电导电泳芯片在有机和无机离子检测中的应用
Y. Chu, Ming-Tai Lu, Hsien-Chang Chang
Conductivity detection can be considered electrochemical technique as well but has the ability to detect any analyte irrespective of whether it contains an or not. The authors used MEMS to fabricate the microelectrode. The microchannel was made by PDMS and modified by O2 plasma. In this study the authors detected organic components such as catechol, dopamine, histamine and vitamin C, and inorganic ions like K+, Li+ and Ca2+. In 30 sec electrokinetic injection time for individual sample detection was performed using a field strength (E) of 440 V/cm, and E = 230 V/cm for separation electrophoresis. Each sample can be detected less than 90 sec excluding catechol.
电导率检测也可以被认为是电化学技术,但它具有检测任何分析物的能力,而不管它是否含有苯胺。采用微机电系统(MEMS)制备微电极。采用PDMS制备微通道,O2等离子体修饰微通道。在这项研究中,作者检测了有机成分,如儿茶酚、多巴胺、组胺和维生素C,以及无机离子,如K+、Li+和Ca2+。在30秒的电动注射时间内对单个样品进行检测,使用电场强度(E)为440 V/cm, E = 230 V/cm进行分离电泳。除儿茶酚外,每个样品可在90秒内检测到。
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引用次数: 0
Characteristics of Cholesteryl Cetyl Carbonate Liquid Crystals as Drug Delivery Systems 胆固醇-十六烷基碳酸酯液晶作为药物传递系统的特性
R. Chuealee, P. Aramwit, T. Srichana
A thermotropic liquid crystals of cholesteryl cetyl carbonate was investigated as a novel nanosystem for delivery of lipophilic drugs. The aims were to synthesize, characterize and develop a formulation for amphotericin B (AmB) in cholesteryl cetyl carbonate liquid crystals. Cholesteryl cetyl carbonate was synthesized and identified by thin layer chromatography (TLC), flash column chromatography, Fourier transformed infrared (FTIR) spectroscopy, mass spectrometry (MS) and Nuclear magnetic resonance spectroscopy (NMR). X-ray diffraction (XRD), transmission electron microscope (TEM), polarized light microscope and differential scanning calorimetry (DSC) were employed to characterize liquid crystals properties. Cholesteryl cetyl carbonate structure is composed of three parts; steroid planar nucleus, hydrocarbon chains and carbonate linkage between the two parts of steroid ring and hydrocarbon chain from fatty alcohol. The phase transition temperatures appeared at around 8 and 36degC and cubic-shaped formation were observed in a micrograph at room temperature. The formulations of AmB in liquid crystals after physical mixing with a sugar revealed high content uniformity and had good characteristics of dry powder forms. It caused low toxicity to red blood cells.
研究了一种热致性的碳酸胆固醇十六烷基酯液晶作为一种新型的亲脂性药物的纳米递送系统。目的是合成、表征和研制一种含胆固醇十六烷基碳酸酯液晶两性霉素B (AmB)的配方。采用薄层色谱(TLC)、闪光柱色谱(flash column chromatography)、傅里叶变换红外光谱(FTIR)、质谱(MS)和核磁共振(NMR)等方法合成了碳酸胆甾醇十六烷基酯,并对其进行了鉴定。采用x射线衍射(XRD)、透射电镜(TEM)、偏振光显微镜(偏振光显微镜)和差示扫描量热法(DSC)表征了液晶的性质。碳酸胆甾醇十六烷基结构由三部分组成;甾烷平面核、烃链和碳酸盐键之间的甾烷环和烃链的两部分来自脂肪醇。相变温度在8°c和36°c左右,室温下显微镜下观察到立方形状的相变。与糖物理混合后的AmB液晶配方具有较高的含量均匀性和良好的干粉形态特征。它对红细胞的毒性很低。
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引用次数: 5
Special Invited Lecture: Integrated MEMS Sensors 特邀讲座:集成MEMS传感器
S. Xia
Monolithic ISFET (ion-sensitive field-effect transistor) pH sensor and microelectrode amperometric immunosensor based on the micro fabrication technology have been studied. The Monolithic ISFET sensor, including the differential ISFET/REFET sensing device and the signal-processing circuit integrated on a single chip, was fabricated by standard CMOS and MEMS technology. Polypyrrole were electropolymerized at different voltage on ISFET gate and REFET gate to form ion-sensitive membrane and ion-insensitive membrane. Micro and compatible amperometric immunosensor, composed of micro electrodes and micro reaction pools, was developed by MEMS technology. Bireceptor molecules were immobilized on the sensitive area of the electrode surface by the technology of self-assembled monolayers. The immunosensor was characterized by detection of immunoglobulin G and ¿-fetoprotein. Both kinds of the sensors show attractive advantages, such as miniaturization, compatibility with CMOS techniques, easy to be designed into micro array and enables relatively rapid, reliable and inexpensive field-analysis.
研究了基于微加工技术的单片离子敏感场效应晶体管(ISFET) pH传感器和微电极安培免疫传感器。采用标准的CMOS和MEMS技术制作了单片ISFET传感器,包括差分ISFET/REFET传感器件和集成在单片上的信号处理电路。将聚吡咯在ISFET栅极和REFET栅极上以不同电压进行电聚合,形成离子敏感膜和离子不敏感膜。采用MEMS技术,研制了由微电极和微反应池组成的微型兼容安培免疫传感器。采用自组装单层技术将双受体分子固定在电极表面的敏感区。该免疫传感器通过检测免疫球蛋白G和-胎儿蛋白进行表征。这两种传感器都具有小型化、与CMOS技术兼容、易于设计成微阵列以及相对快速、可靠和廉价的现场分析等优点。
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引用次数: 0
Rapid Fabrication of CNT Sensors Using Electro-chemical Deposition of Functionalized CNTs 电化学沉积功能化碳纳米管快速制备碳纳米管传感器
W. Chow, M. Wong, W.J. Li, K. Wong
We present a novel fabrication technique to fabricate functionalized CNT sensors rapidly using electrochemical deposition method. The basic fabrication process of this sensor includes fabrication of a gold (Au) microelectrode array by photolithography process, functionalization of multi-walled carbon nanotubes (MWNTs) with carboxylic acid groups (-COOH), and electro-chemical deposition of functionalized MWNTs (f-CNTs) on the Au microelectrode array. The adhesion between the f-CNTs and the Au microelectrodes could be enhanced if CNT sensors are built using the process described in this paper. The I-V characteristics of the sensors were investigated. Our experimental results show that CNT sensors fabricated by electro-chemical process have dramatically different I-V characteristics compared with sensors fabricated by DEP or AFM manipulation techniques. The power limit of the sensors ranges from 0.28mW to 6.21mW, which is much higher than most reported CNT sensors. Self-heating effect can be induced at input power of ~2.2mW. Based on these experimental results, we think that the novel f-CNT sensors should be investigated further for applications in thermal, mechanical, and biomedical systems.
提出了一种利用电化学沉积法快速制备功能化碳纳米管传感器的新方法。该传感器的基本制造工艺包括通过光刻工艺制备金(Au)微电极阵列,用羧酸基(-COOH)将多壁碳纳米管(MWNTs)功能化,以及在金微电极阵列上电化学沉积功能化的MWNTs (f-CNTs)。如果采用本文描述的工艺构建碳纳米管传感器,则可以增强f-CNTs与Au微电极之间的粘附性。研究了传感器的I-V特性。实验结果表明,电化学工艺制备的碳纳米管传感器与DEP或AFM工艺制备的传感器具有显著不同的I-V特性。传感器的功率限制范围为0.28mW至6.21mW,远远高于大多数CNT传感器。当输入功率为~2.2mW时,可产生自热效应。基于这些实验结果,我们认为新型的f-CNT传感器应该进一步研究在热、机械和生物医学系统中的应用。
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引用次数: 3
Synthesis of High Quality ZnO Nanorods by Low Temperature Wet Chemical Process 低温湿化学法制备高品质ZnO纳米棒
K. Lee, K. Park, K. Koh, Soonil Lee
The authors synthesized high quality ZnO nanorods on Au-and ZnO-coated ITO substrates using a low temperature wet chemical process. Scanning electron microscopy and X-ray diffraction showed that ZnO nanorods were vertically aligned on both type of substrates. Room temperature photoluminescence (PL) measurements showed a strong band-edge emission at ~380 nm for ZnO nanorods on both type of substrates, but substrate-dependent difference was observed in weak and broad visible emissions. Through the careful and systematic analysis of PL spectra, the authors confirmed that the apparent peak at ~760 nm were nothing but the second-order feature of the band-edge emission. Finally, the authors took advantage the selectivity of ZnO-nanorod growth on particular surfaces to demonstrate the possibility for pattered growth.
采用低温湿法在镀有au和ZnO的ITO衬底上合成了高质量的ZnO纳米棒。扫描电镜和x射线衍射结果表明,ZnO纳米棒在两种衬底上呈垂直排列。室温光致发光(PL)测量表明,ZnO纳米棒在两种类型的衬底上都有~380 nm的强带边发射,但在弱可见光和宽可见光发射上存在衬底相关的差异。通过对PL光谱的细致系统分析,证实了~760 nm处的视峰为带边发射的二阶特征。最后,作者利用zno纳米棒在特定表面上生长的选择性来证明图案生长的可能性。
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引用次数: 1
Nanopatterning with Extreme Ultraviolet Interference Lithography for Nanoelectronics and Biotechnology 纳米电子学和生物技术的极紫外干涉光刻纳米图案
B. Stadler, H. Solak, S. Frerker, M. Grandin, J. Voros
We present a platform for the controlled patterning of nano-objects such as gold colloids in the micron and nanorange. We are separating the lithography step from the subsequent functionalization. This has many advantages among them the independence of the linkage chemistry and the lithography process and the possibility to position individual nano-objects. The lithography step is extreme UV interference lithography in the nanorange and standard photolithography in the micron range. As a proof of concept, micro and nanopatterns of single-stranded DNAs are created in an inert background of PLL-g-PEG. The DNA patterns are converted to arrays of DNA-tagged gold colloids by directed self-assembly using the specificity of DNA hybridization. Especially nanoline arrays of gold colloids have the potential to serve as e.g. nanowires for electrical label-free biosensing. In addition, we also present the characterization of the adsorption kinetics of 20 nm DNA-tagged gold colloids via SPR.
我们提出了一个平台,以控制图案的纳米物体,如金胶体在微米和纳米橙。我们正在将光刻步骤与随后的功能化步骤分开。这有许多优点,其中包括连接化学和光刻工艺的独立性,以及单个纳米物体定位的可能性。光刻步骤为纳米级的极紫外干涉光刻和微米级的标准光刻。作为概念的证明,单链dna的微观和纳米模式是在PLL-g-PEG的惰性背景下创建的。利用DNA杂交的特异性,通过定向自组装将DNA模式转换为DNA标记的金胶体阵列。特别是金胶体的纳米线阵列有潜力作为无标签生物传感的纳米线。此外,我们还通过SPR表征了20 nm dna标记金胶体的吸附动力学。
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引用次数: 1
An Inductance-based Sensor for DNA Hybridization Detection 一种基于电感的DNA杂交检测传感器
S. Azimi, M. R. Bahmanyar, M. Zolgharni, W. Balachandran
This paper introduces an inductance-based sensor for detection of DNA hybridization and investigates its performance by means of computer simulation. In order to detect the occurrence of hybridization, single strand target DNA's are tagged with magnetic beads. Target DNA's are then exposed to known single strand probe DNA's which are immobilized on a surface in the proximity of a spiral coil with a specific design. After hybridization, the expected variations in the coil inductance due to presence of magnetic beads are studied for different coils as well as magnetic beads of different sizes and permeabilities. The simulation results are presented and discussed in order to obtain optimal coil parameters with the aim of producing maximum variations in the coil inductance.
本文介绍了一种用于DNA杂交检测的电感式传感器,并通过计算机仿真对其性能进行了研究。为了检测杂交的发生,用磁珠标记单链靶DNA。然后将目标DNA暴露于已知的单链探针DNA中,这些探针DNA固定在具有特定设计的螺旋线圈附近的表面上。杂交后,研究了不同线圈、不同尺寸和磁珠导通率的磁珠的存在对线圈电感的预期变化。为了获得最优的线圈参数,使线圈电感变化最大,给出了仿真结果并进行了讨论。
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引用次数: 9
Enhancement in Electrical Conductivity of Transparent Single-Walled Carbon Nanotube Films 透明单壁碳纳米管薄膜电导率的增强
Byung-Seon Kong, Hee‐Tae Jung, Sounghee Park, Moon-ki Park
We have developed a simple and practical method to enhance the electrical conductivity of single-walled carbon nanotube (SWNT) films without loss of their transparency. In order to achieve an enhanced conductivity of transparent SWNT films, we carried out the formation of gold nanoparticles through the reduction of gold ions (Au3+) onto the SWNT films which manufactured by the vacuum filtration method. The electroless reduction of gold ions results from redox reactions between Au3+ and SWNT sidewalls just by immersion bare SWNT films into an ethanolic aqueous solution of gold salt (HAuCl4) for several minutes. After the formation of gold nanoparticles, the electrical conductivity increased over two-fold of the initial conductivity and maintained the visible light transmittance ~80 %.
我们开发了一种简单实用的方法来提高单壁碳纳米管(SWNT)薄膜的导电性而不损失其透明度。为了提高透明SWNT薄膜的导电性,我们将金离子(Au3+)还原到真空过滤法制备的SWNT薄膜上,形成了金纳米颗粒。金离子的化学还原是由Au3+与SWNT侧壁之间的氧化还原反应引起的,只需将裸露的SWNT膜浸入金盐(HAuCl4)的乙醇水溶液中几分钟。金纳米颗粒形成后,电导率比初始电导率提高2倍以上,可见光透过率维持在80%左右。
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引用次数: 0
期刊
2007 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems
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