Pub Date : 2007-04-23DOI: 10.1109/nems.2007.352160
Si-Hyung Lim, J. Jaworski, S. Satyanarayana, F. Wang, D. Raorane, Seung-Wuk Lee, A. Majumdar
For gas phase chemical sensing, we have developed a selective, sensitive and portable sensing platform, which integrates bio receptors, chemo-mechanical sensor array, and electrical readout circuits. The biggest challenge in chemical sensors is selectivity of a receptor to its respective target molecule against a background of various interfering agents. For target specific receptors, sequence-specific recognition motifs have been identified through directed evolution methods, called phage display. We have demonstrated and updated a parylene micromembrane surface stress sensor array which uses capacitive signal readout. For a fully integrated sensor platform, a portable chemical sensing board has been built.
{"title":"Nanomechanical Chemical Sensor Platform","authors":"Si-Hyung Lim, J. Jaworski, S. Satyanarayana, F. Wang, D. Raorane, Seung-Wuk Lee, A. Majumdar","doi":"10.1109/nems.2007.352160","DOIUrl":"https://doi.org/10.1109/nems.2007.352160","url":null,"abstract":"For gas phase chemical sensing, we have developed a selective, sensitive and portable sensing platform, which integrates bio receptors, chemo-mechanical sensor array, and electrical readout circuits. The biggest challenge in chemical sensors is selectivity of a receptor to its respective target molecule against a background of various interfering agents. For target specific receptors, sequence-specific recognition motifs have been identified through directed evolution methods, called phage display. We have demonstrated and updated a parylene micromembrane surface stress sensor array which uses capacitive signal readout. For a fully integrated sensor platform, a portable chemical sensing board has been built.","PeriodicalId":364039,"journal":{"name":"2007 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems","volume":"34 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128134323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2007-04-23DOI: 10.1109/NEMS.2007.352093
Weiping Chen, J. Ding, Xiaowei Liu, Chao Wang
A system-level model of a bulk micromachined dual axis accelerometer is presented. The accelerometer has only one inertial mass, symmetrically suspended by four pairs of folded elastic beams. The size of the fabricated accelerometer is 5.4times5.4 mm2. The displacement change of the inertial mass is converted to the change of differential capacitance between active combs and fixed combs. Generally, the input acceleration is mixed with the x-axis and the y-axis. In order to separate and pick off the mixed signal, the frequency-division method is adopted in the interface circuit. The paper details the Simulink model, which is used to evaluate the feasibility of the detective method. The simulation results validate the feasibility that the interface circuit can detect dual-axis acceleration separately and synchronously.
{"title":"Design and system-level simulation of a capacitive dual axis accelerometer","authors":"Weiping Chen, J. Ding, Xiaowei Liu, Chao Wang","doi":"10.1109/NEMS.2007.352093","DOIUrl":"https://doi.org/10.1109/NEMS.2007.352093","url":null,"abstract":"A system-level model of a bulk micromachined dual axis accelerometer is presented. The accelerometer has only one inertial mass, symmetrically suspended by four pairs of folded elastic beams. The size of the fabricated accelerometer is 5.4times5.4 mm2. The displacement change of the inertial mass is converted to the change of differential capacitance between active combs and fixed combs. Generally, the input acceleration is mixed with the x-axis and the y-axis. In order to separate and pick off the mixed signal, the frequency-division method is adopted in the interface circuit. The paper details the Simulink model, which is used to evaluate the feasibility of the detective method. The simulation results validate the feasibility that the interface circuit can detect dual-axis acceleration separately and synchronously.","PeriodicalId":364039,"journal":{"name":"2007 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems","volume":"74 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132600583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2007-04-23DOI: 10.1109/NEMS.2007.352112
W. Lou, X. Yu
MEMS package technology is playing an increasingly important part today. As one of important part of MEMS package technology, flip-chip technology is widely used in the assembly of high-performance that requires good functionality on substrate space. It has many advantages such as smaller size, increased functionality and lower cost. So it has been widely used in MEMS package. The thermal stress of flip-chip is analyzed by using the finite element analysis software in this paper. It simulates the thermal stress distributing of the flip-chip structure, which is caused by the high temperature during the underfill process. And by modifying all kinds of geometry parameters and material attributes, it analyses that how underfill CTE, solidifying temperature and bump size affect the thermal stress of the micro-structure. The results from this work would be very useful to optimize the technological parameter and improve the package properties.
{"title":"Flip-Chip Micro-Thermal Stress Simulation in Underfill Process","authors":"W. Lou, X. Yu","doi":"10.1109/NEMS.2007.352112","DOIUrl":"https://doi.org/10.1109/NEMS.2007.352112","url":null,"abstract":"MEMS package technology is playing an increasingly important part today. As one of important part of MEMS package technology, flip-chip technology is widely used in the assembly of high-performance that requires good functionality on substrate space. It has many advantages such as smaller size, increased functionality and lower cost. So it has been widely used in MEMS package. The thermal stress of flip-chip is analyzed by using the finite element analysis software in this paper. It simulates the thermal stress distributing of the flip-chip structure, which is caused by the high temperature during the underfill process. And by modifying all kinds of geometry parameters and material attributes, it analyses that how underfill CTE, solidifying temperature and bump size affect the thermal stress of the micro-structure. The results from this work would be very useful to optimize the technological parameter and improve the package properties.","PeriodicalId":364039,"journal":{"name":"2007 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems","volume":"52 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133632048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2007-04-23DOI: 10.1109/NEMS.2007.352194
B. Chu, E. Pierstorff, D. Ho
We have utilized a block copolymeric thin film as a modality to template the deposition of single-walled carbon nanotubes towards applications in single cell interrogation. Transmembrane studies of cellular activity (e.g. neurons, cardiomyocytes, etc.) have often been limited by the invasiveness of probe-induced membrane rupture. This often precludes chronic activity analysis. We have developed a copolymer-carbon nanotube (P-CNT) hybrid material for potential applications in non-invasive cell probing with attenuated inflammation due to the biomimetic stiffness of the copolymer coupled with nanoscale dimensions of the P-CNT complex. We applied both a diblock copolymer comprised of poly(ethylene oxide-b-methyl methacrylate; PEO-PMMA) as well as an acrylate-terminated amphiphilic `ABA' triblock copolymer comprised of polymethyloxazoline-polydimethylsiloxane-polymethyloxazoline; PMOXA-PDMS-PMOXA) as the supporting matrix for carbon nanotube deposition via the Langmuir-Blodgett methodology. This enabled the suspension of the carbon nanotubes on the air-water interface for transfer to a gold substrate. Cyclic voltammetry measurements confirmed that the CNT's were interfaced directly with the gold substrates to enable electrical functionality. In addition, cellular adhesion to the polymeric substrate was demonstrated, confirming the biocompatibility of the P-CNT material. CNT-coated electrodes were also examined as biological electrodes for the monitoring of oxidation-reduction processes driven by the cytochrome c mediator, where CNT/polymer-coated surfaces were also capable of facilitating anti-protein adsorption, resulting in the observation of reversible electron transfer between the protein and electrode. This was demonstrated via acquisition of pronounced anodic and cathodic peaks with peak separations of 64mV, which confirmed a reversible transfer process.
{"title":"Polymer-Enabled Carbon Nanotube Deposition for Cellular Interrogation Applications","authors":"B. Chu, E. Pierstorff, D. Ho","doi":"10.1109/NEMS.2007.352194","DOIUrl":"https://doi.org/10.1109/NEMS.2007.352194","url":null,"abstract":"We have utilized a block copolymeric thin film as a modality to template the deposition of single-walled carbon nanotubes towards applications in single cell interrogation. Transmembrane studies of cellular activity (e.g. neurons, cardiomyocytes, etc.) have often been limited by the invasiveness of probe-induced membrane rupture. This often precludes chronic activity analysis. We have developed a copolymer-carbon nanotube (P-CNT) hybrid material for potential applications in non-invasive cell probing with attenuated inflammation due to the biomimetic stiffness of the copolymer coupled with nanoscale dimensions of the P-CNT complex. We applied both a diblock copolymer comprised of poly(ethylene oxide-b-methyl methacrylate; PEO-PMMA) as well as an acrylate-terminated amphiphilic `ABA' triblock copolymer comprised of polymethyloxazoline-polydimethylsiloxane-polymethyloxazoline; PMOXA-PDMS-PMOXA) as the supporting matrix for carbon nanotube deposition via the Langmuir-Blodgett methodology. This enabled the suspension of the carbon nanotubes on the air-water interface for transfer to a gold substrate. Cyclic voltammetry measurements confirmed that the CNT's were interfaced directly with the gold substrates to enable electrical functionality. In addition, cellular adhesion to the polymeric substrate was demonstrated, confirming the biocompatibility of the P-CNT material. CNT-coated electrodes were also examined as biological electrodes for the monitoring of oxidation-reduction processes driven by the cytochrome c mediator, where CNT/polymer-coated surfaces were also capable of facilitating anti-protein adsorption, resulting in the observation of reversible electron transfer between the protein and electrode. This was demonstrated via acquisition of pronounced anodic and cathodic peaks with peak separations of 64mV, which confirmed a reversible transfer process.","PeriodicalId":364039,"journal":{"name":"2007 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems","volume":"28 47","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114050204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2007-04-23DOI: 10.1109/NEMS.2007.352243
Yuhong Long, Liangcai Xiong, T. Shi, Zirong Tang
To further understand the behavior of laser-induced electrochemical etching process, the experiments of micromachining silicon by laser-induced electrochemical etching were carried out. 248nm excimer laser as light source is adopted in this work with the power of 109W/cm2 for the first time and KOH solution is used as electrolyte. Based on the experiment results, the surface images and etching rate are analyzed in detail. It is verified that the compound technique is a combination of laser etching, electrochemical etching and coupling etching, and laser etching is dominating in the compound process. Besides, both liquid-enhanced pressure and jet shock pressure can preferably improve the etching rate. At the same time, the anisotropic etching stop of silicon in alkaline solution is solved in this study. As a result, this process can be applied to transfer pattern without mask, and it possesses the ability of machining large aspect ratio micro structures.
{"title":"Study of Excimer Laser Electrochemical Etching Silicon","authors":"Yuhong Long, Liangcai Xiong, T. Shi, Zirong Tang","doi":"10.1109/NEMS.2007.352243","DOIUrl":"https://doi.org/10.1109/NEMS.2007.352243","url":null,"abstract":"To further understand the behavior of laser-induced electrochemical etching process, the experiments of micromachining silicon by laser-induced electrochemical etching were carried out. 248nm excimer laser as light source is adopted in this work with the power of 109W/cm2 for the first time and KOH solution is used as electrolyte. Based on the experiment results, the surface images and etching rate are analyzed in detail. It is verified that the compound technique is a combination of laser etching, electrochemical etching and coupling etching, and laser etching is dominating in the compound process. Besides, both liquid-enhanced pressure and jet shock pressure can preferably improve the etching rate. At the same time, the anisotropic etching stop of silicon in alkaline solution is solved in this study. As a result, this process can be applied to transfer pattern without mask, and it possesses the ability of machining large aspect ratio micro structures.","PeriodicalId":364039,"journal":{"name":"2007 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems","volume":"33 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115081582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2007-04-23DOI: 10.1109/NEMS.2007.352008
O. Tabata
The role of University in MNT has been becoming more and more essential since the Micro Nano Technology (MNT) requires not only innovative seeds to open up a new application fields but also both well-qualified persons in MNT who lead this field. To meet these requirements, interdisciplinary and international collaboration and cooperation in education and research is crucially important. Firstly, part of related activities related to MNT in Kyoto University are introduced, such as the education program in MNT, the organization of newly established Micro Engineering Department, and research organization of Research Institute of Nano Science & Technology which is the lateral organization of several departments and colleges on nanotechnology. Secondly, two research topics in MNT, nano-scale material mechanical property characterization and nano-components assembly on MEMS which are pursued at Micro Nano System Laboratory are introduced as the tentative themes for further international collaboration and cooperation.
{"title":"Workshop Speech: Role of University Research for Open Innovations in MNT","authors":"O. Tabata","doi":"10.1109/NEMS.2007.352008","DOIUrl":"https://doi.org/10.1109/NEMS.2007.352008","url":null,"abstract":"The role of University in MNT has been becoming more and more essential since the Micro Nano Technology (MNT) requires not only innovative seeds to open up a new application fields but also both well-qualified persons in MNT who lead this field. To meet these requirements, interdisciplinary and international collaboration and cooperation in education and research is crucially important. Firstly, part of related activities related to MNT in Kyoto University are introduced, such as the education program in MNT, the organization of newly established Micro Engineering Department, and research organization of Research Institute of Nano Science & Technology which is the lateral organization of several departments and colleges on nanotechnology. Secondly, two research topics in MNT, nano-scale material mechanical property characterization and nano-components assembly on MEMS which are pursued at Micro Nano System Laboratory are introduced as the tentative themes for further international collaboration and cooperation.","PeriodicalId":364039,"journal":{"name":"2007 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems","volume":"77 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114746411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2007-04-23DOI: 10.1109/NEMS.2007.352247
Tiantian Zhang, L. Jia
A three-dimensional compressible model of microchannel was established. Flow and heat transfer characteristics of nitrogen flows in microchannels (hydraulic diameter is 100 mum) with designed roughness in form of obstructions placed along channels walls have been investigated numerically through a finite element CFD code consideration of the effect of compressibility and viscosity heating. The model was verified through comparing with experiment data. Then the effects of the Reynolds number, obstruction height, obstruction pitch, obstruction geometry and width to height Ratio of obstruction on the flow and heat transfer characteristics were investigated.
{"title":"Numerical Simulation of Roughness Effect on Gaseous Flow and Heat Transfer in Microchannels","authors":"Tiantian Zhang, L. Jia","doi":"10.1109/NEMS.2007.352247","DOIUrl":"https://doi.org/10.1109/NEMS.2007.352247","url":null,"abstract":"A three-dimensional compressible model of microchannel was established. Flow and heat transfer characteristics of nitrogen flows in microchannels (hydraulic diameter is 100 mum) with designed roughness in form of obstructions placed along channels walls have been investigated numerically through a finite element CFD code consideration of the effect of compressibility and viscosity heating. The model was verified through comparing with experiment data. Then the effects of the Reynolds number, obstruction height, obstruction pitch, obstruction geometry and width to height Ratio of obstruction on the flow and heat transfer characteristics were investigated.","PeriodicalId":364039,"journal":{"name":"2007 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117061767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2007-04-23DOI: 10.1109/NEMS.2007.352001
C. Chung, T.S. Chen, C. Peng, B.H. Wu
In this paper, the morphology and properties of nanostructured Ta-Si-N thin films fabricated by reactively cosputtering have been studied. The Ta-Si-N film is a mixed composite consisting of the Ta-Si, Ta-N and Si-N compounds. The TaN phase is polycrystalline while SiNx is amorphous. As Si is added to the Ta-N compound to form Ta-Si-N, the micro structure becomes nanocrystalline grains embedded in an amorphous matrix i.e. amorphous-like micro structure, which is also affected by the nitrogen flow ratio i.e. FN2%= FN2/( FN2+FAr) times 100% during sputtering. Amorphous-like Ta-Si-N films obtained at small FN2% of 2-10% had smaller roughness, lower resistivity and larger nanohardness compared to polycrystalline films at high FN2% of 20- 30%. The variation of Ta-Si-N micro structure leads to the different electrical and mechanical properties of films. The electric resistivity of Ta-Si-N increases with increasing FN2% while the nanohardness first increases to a maximum of 15.19 GPa from FN2% of 2% to 3%, then decreases with increasing FN2%. The higher hardness in amorphous-like Ta-Si-N exhibits a larger stiffness and resilience than polycrystalline one.
{"title":"Fabrication and Characterization of Nanostructured Ta-Si-N Films","authors":"C. Chung, T.S. Chen, C. Peng, B.H. Wu","doi":"10.1109/NEMS.2007.352001","DOIUrl":"https://doi.org/10.1109/NEMS.2007.352001","url":null,"abstract":"In this paper, the morphology and properties of nanostructured Ta-Si-N thin films fabricated by reactively cosputtering have been studied. The Ta-Si-N film is a mixed composite consisting of the Ta-Si, Ta-N and Si-N compounds. The TaN phase is polycrystalline while SiNx is amorphous. As Si is added to the Ta-N compound to form Ta-Si-N, the micro structure becomes nanocrystalline grains embedded in an amorphous matrix i.e. amorphous-like micro structure, which is also affected by the nitrogen flow ratio i.e. FN2%= FN2/( FN2+FAr) times 100% during sputtering. Amorphous-like Ta-Si-N films obtained at small FN2% of 2-10% had smaller roughness, lower resistivity and larger nanohardness compared to polycrystalline films at high FN2% of 20- 30%. The variation of Ta-Si-N micro structure leads to the different electrical and mechanical properties of films. The electric resistivity of Ta-Si-N increases with increasing FN2% while the nanohardness first increases to a maximum of 15.19 GPa from FN2% of 2% to 3%, then decreases with increasing FN2%. The higher hardness in amorphous-like Ta-Si-N exhibits a larger stiffness and resilience than polycrystalline one.","PeriodicalId":364039,"journal":{"name":"2007 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117091243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2007-04-23DOI: 10.1109/NEMS.2007.352045
D. Grieshaber, E. Reimhult, J. Voros
Enzymatic biosensors are expected to play a key-role in bio techno logical and biochemical analysis as shown by the success of glucose sensors in diabetes treatment. The aim of this work is to develop a multiplexed electronic detection system for early cancer diagnostics. Therefore, various enzymes were adsorbed to differently modified surfaces. Electrochemical optical waveguide lightmode spectroscopy (EC-OWLS) and electrochemical quartz crystal microbalance with dissipation (EC-QCM-D) were used to measure the mass and the activity of the adsorbed enzymes. The enzymes were specifically immobilized on a protein resistant PLL-g-PEG surface in order to reduce the loss of activity due to denaturation. In addition, enzymes were also incorporated into DNA-tagged vesicles to increase the signal and therefore the sensor sensitivity. The enzymatic activity of the different systems was compared. To further increase the sensitivity, ferrocyanide was used as an electron mediator.
{"title":"Enzymatic Biosensors towards a Multiplexed Electronic Detection System for Early Cancer Diagnostics","authors":"D. Grieshaber, E. Reimhult, J. Voros","doi":"10.1109/NEMS.2007.352045","DOIUrl":"https://doi.org/10.1109/NEMS.2007.352045","url":null,"abstract":"Enzymatic biosensors are expected to play a key-role in bio techno logical and biochemical analysis as shown by the success of glucose sensors in diabetes treatment. The aim of this work is to develop a multiplexed electronic detection system for early cancer diagnostics. Therefore, various enzymes were adsorbed to differently modified surfaces. Electrochemical optical waveguide lightmode spectroscopy (EC-OWLS) and electrochemical quartz crystal microbalance with dissipation (EC-QCM-D) were used to measure the mass and the activity of the adsorbed enzymes. The enzymes were specifically immobilized on a protein resistant PLL-g-PEG surface in order to reduce the loss of activity due to denaturation. In addition, enzymes were also incorporated into DNA-tagged vesicles to increase the signal and therefore the sensor sensitivity. The enzymatic activity of the different systems was compared. To further increase the sensitivity, ferrocyanide was used as an electron mediator.","PeriodicalId":364039,"journal":{"name":"2007 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115462691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2007-04-23DOI: 10.1109/NEMS.2007.352209
Chi-Chang Lin, Jr-Tzai Chen, Yan-Fu Chen, T. Chang, Hsien-Chang Chang
An unique, sensitive, and highly specific immunoassay system using gold nanoparticles and surface-enhanced Raman spectroscopy (SERS) is described. To demonstrate the analytical capabilities of the new technique, Raman reporter (mercaptobenzoic, MBA) that is coimmobilized with biospeciflc anti-protein A on gold nanoparticles (AuNP). Anti-protein A-AuNP-MBA, which combing both electromagnetic field and chemical enhancement, was used to exploits the SERS-derived signal. A dynamic range of 2-3 orders of magnitude and 1-10 pg/mL of detection limitation of protein A were achieved. The results indicated this new technique could be used in clinical diagnostic applications with fast, high sensitivity and high-throughput screening of antibodies.
{"title":"Immunogold Nanoparticle Combing Surface-Enhanced Raman Scattering Method for Protein A Detection","authors":"Chi-Chang Lin, Jr-Tzai Chen, Yan-Fu Chen, T. Chang, Hsien-Chang Chang","doi":"10.1109/NEMS.2007.352209","DOIUrl":"https://doi.org/10.1109/NEMS.2007.352209","url":null,"abstract":"An unique, sensitive, and highly specific immunoassay system using gold nanoparticles and surface-enhanced Raman spectroscopy (SERS) is described. To demonstrate the analytical capabilities of the new technique, Raman reporter (mercaptobenzoic, MBA) that is coimmobilized with biospeciflc anti-protein A on gold nanoparticles (AuNP). Anti-protein A-AuNP-MBA, which combing both electromagnetic field and chemical enhancement, was used to exploits the SERS-derived signal. A dynamic range of 2-3 orders of magnitude and 1-10 pg/mL of detection limitation of protein A were achieved. The results indicated this new technique could be used in clinical diagnostic applications with fast, high sensitivity and high-throughput screening of antibodies.","PeriodicalId":364039,"journal":{"name":"2007 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124908635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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