Guoyu Hu, Danqiu Zhang, Dan Luo, Wenhui Sun, Rijin Zhou, Zonglie Hong, Shoaib Munir, Zhibiao Ye, Changxian Yang, Junhong Zhang, Taotao Wang
The complexity of compound leaves results primarily from the leaflet initiation and arrangement during leaf development. However, the molecular mechanism underlying compound leaf development remains a central research question.
SlTCP24 and SlTCP29, two plant-specific transcription factors with the conserved TCP motif, are shown here to synergistically regulate compound leaf development in tomato. When both of them were knocked out simultaneously, the number of leaflets significantly increased, and the shape of the leaves became more complex. SlTCP24 and SlTCP29 could form both homodimers and heterodimers, and such dimerization was impeded by the leaf polarity regulator SlAS2, which interacted with SlTCP24 and SlTCP29.
SlTCP24 and SlTCP29 could bind to the TCP-binding cis-element of the SlCKX2 promoter and activate its transcription. Transgenic plants with SlTCP24 and SlTCP29 double-gene knockout had a lowered transcript level of SlCKX2 and an elevated level of cytokinin.
This work led to the identification of two key regulators of tomato compound leaf development and their targeted genes involved in cytokinin metabolic pathway. A model of regulation of compound leaf development was proposed based on observations of this study.
{"title":"SlTCP24 and SlTCP29 synergistically regulate compound leaf development through interacting with SlAS2 and activating transcription of SlCKX2 in tomato","authors":"Guoyu Hu, Danqiu Zhang, Dan Luo, Wenhui Sun, Rijin Zhou, Zonglie Hong, Shoaib Munir, Zhibiao Ye, Changxian Yang, Junhong Zhang, Taotao Wang","doi":"10.1111/nph.19221","DOIUrl":"https://doi.org/10.1111/nph.19221","url":null,"abstract":"<div>\u0000 \u0000 <p>\u0000 \u0000 </p><ul>\u0000 \u0000 \u0000 <li>The complexity of compound leaves results primarily from the leaflet initiation and arrangement during leaf development. However, the molecular mechanism underlying compound leaf development remains a central research question.</li>\u0000 \u0000 \u0000 <li>SlTCP24 and SlTCP29, two plant-specific transcription factors with the conserved TCP motif, are shown here to synergistically regulate compound leaf development in tomato. When both of them were knocked out simultaneously, the number of leaflets significantly increased, and the shape of the leaves became more complex. SlTCP24 and SlTCP29 could form both homodimers and heterodimers, and such dimerization was impeded by the leaf polarity regulator SlAS2, which interacted with SlTCP24 and SlTCP29.</li>\u0000 \u0000 \u0000 <li>SlTCP24 and SlTCP29 could bind to the TCP-binding <i>cis</i>-element of the <i>SlCKX2</i> promoter and activate its transcription. Transgenic plants with <i>SlTCP24</i> and <i>SlTCP29</i> double-gene knockout had a lowered transcript level of <i>SlCKX2</i> and an elevated level of cytokinin.</li>\u0000 \u0000 \u0000 <li>This work led to the identification of two key regulators of tomato compound leaf development and their targeted genes involved in cytokinin metabolic pathway. A model of regulation of compound leaf development was proposed based on observations of this study.</li>\u0000 </ul>\u0000 \u0000 </div>","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":"240 3","pages":"1275-1291"},"PeriodicalIF":9.4,"publicationDate":"2023-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41087812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Zhichao Xia, Yue He, Helena Korpelainen, Ülo Niinemets, Chunyang Li
Little is known about how sex differences in root zone characteristics, such as contents of allelochemicals and soil microbial composition, mediate intra- and intersexual interactions in dioecious plants.
We examined the processes and mechanisms of sex-specific belowground interactions mediated by allelochemicals and soil microorganisms in Populus cathayana females and males in replicated 30-yr-old experimental stands in situ and in a series of controlled experiments.
Female roots released a greater amount and more diverse phenolic allelochemicals into the soil environment, resulting in growth inhibition of the same sex neighbors and deterioration of the community of soil microorganisms. When grown with males, the growth of females was consistently enhanced, especially the root growth. Compared with female monocultures, the presence of males reduced the total phenolic accumulation in the soil, resulting in a shift from allelopathic inhibition to chemical facilitation. This association was enhanced by a favorable soil bacterial community and increased bacterial diversity, and it induced changes in the orientation of female roots.
Our study highlighted a novel mechanism that enhances female performance by males through alterations in the allelochemical content and soil microbial composition. The possibility to improve productivity by chemical mediation provides novel opportunities for managing plantations of dioecious plants.
{"title":"Allelochemicals and soil microorganisms jointly mediate sex-specific belowground interactions in dioecious Populus cathayana","authors":"Zhichao Xia, Yue He, Helena Korpelainen, Ülo Niinemets, Chunyang Li","doi":"10.1111/nph.19224","DOIUrl":"10.1111/nph.19224","url":null,"abstract":"<div>\u0000 \u0000 <p>\u0000 \u0000 </p><ul>\u0000 \u0000 \u0000 <li>Little is known about how sex differences in root zone characteristics, such as contents of allelochemicals and soil microbial composition, mediate intra- and intersexual interactions in dioecious plants.</li>\u0000 \u0000 \u0000 <li>We examined the processes and mechanisms of sex-specific belowground interactions mediated by allelochemicals and soil microorganisms in <i>Populus cathayana</i> females and males in replicated 30-yr-old experimental stands <i>in situ</i> and in a series of controlled experiments.</li>\u0000 \u0000 \u0000 <li>Female roots released a greater amount and more diverse phenolic allelochemicals into the soil environment, resulting in growth inhibition of the same sex neighbors and deterioration of the community of soil microorganisms. When grown with males, the growth of females was consistently enhanced, especially the root growth. Compared with female monocultures, the presence of males reduced the total phenolic accumulation in the soil, resulting in a shift from allelopathic inhibition to chemical facilitation. This association was enhanced by a favorable soil bacterial community and increased bacterial diversity, and it induced changes in the orientation of female roots.</li>\u0000 \u0000 \u0000 <li>Our study highlighted a novel mechanism that enhances female performance by males through alterations in the allelochemical content and soil microbial composition. The possibility to improve productivity by chemical mediation provides novel opportunities for managing plantations of dioecious plants.</li>\u0000 </ul>\u0000 \u0000 </div>","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":"240 4","pages":"1519-1533"},"PeriodicalIF":9.4,"publicationDate":"2023-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10416026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Haibin Jiang, Yeqiang Xia, Sicong Zhang, Zhichao Zhang, Hui Feng, Qi Zhang, Xi Chen, Junhua Xiao, Sen Yang, Mengzhu Zeng, Zhaodan Chen, Haibing Ouyang, Xinyi He, Guangzheng Sun, Jinbin Wu, Suomeng Dong, Wenwu Ye, Zhenchuan Ma, Yan Wang, Yuanchao Wang
The role of cysteine-rich secretory proteins, antigen 5, and pathogenesis-related 1 (CAP) superfamily proteins in the innate immune responses of mammals is well characterized. However, the biological function of CAP superfamily proteins in plant–microbe interactions is poorly understood.
We used proteomics and transcriptome analyses to dissect the apoplastic effectors secreted by the oomycete Phytophthora sojae during early infection of soybean leaves. By transiently expressing these effectors in Nicotiana benthamiana, we identified PsCAP1, a novel type of secreted CAP protein that triggers immune responses in multiple solanaceous plants including N. benthamiana. This secreted CAP protein is conserved among oomycetes, and multiple PsCAP1 homologs can be recognized by N. benthamiana.
PsCAP1-triggered immune responses depend on the N-terminal immunogenic fragment (aa 27–151). Pretreatment of N. benthamiana with PsCAP1 or the immunogenic fragment increases plant resistance against Phytophthora. The recognition of PsCAP1 and different homologs requires the leucine-rich repeat receptor-like protein RCAP1, which associates with two central receptor-like kinases BRI1-associated receptor kinase 1 (BAK1) and suppressor of BIR1-1 (SOBIR1) in planta.
These findings suggest that the CAP-type apoplastic effectors act as an important player in plant–microbe interactions that can be perceived by plant membrane-localized receptor to activate plant resistance.
{"title":"The CAP superfamily protein PsCAP1 secreted by Phytophthora triggers immune responses in Nicotiana benthamiana through a leucine-rich repeat receptor-like protein","authors":"Haibin Jiang, Yeqiang Xia, Sicong Zhang, Zhichao Zhang, Hui Feng, Qi Zhang, Xi Chen, Junhua Xiao, Sen Yang, Mengzhu Zeng, Zhaodan Chen, Haibing Ouyang, Xinyi He, Guangzheng Sun, Jinbin Wu, Suomeng Dong, Wenwu Ye, Zhenchuan Ma, Yan Wang, Yuanchao Wang","doi":"10.1111/nph.19194","DOIUrl":"https://doi.org/10.1111/nph.19194","url":null,"abstract":"<div>\u0000 \u0000 <p>\u0000 </p><ul>\u0000 \u0000 <li>The role of cysteine-rich secretory proteins, antigen 5, and pathogenesis-related 1 (CAP) superfamily proteins in the innate immune responses of mammals is well characterized. However, the biological function of CAP superfamily proteins in plant–microbe interactions is poorly understood.</li>\u0000 \u0000 <li>We used proteomics and transcriptome analyses to dissect the apoplastic effectors secreted by the oomycete <i>Phytophthora sojae</i> during early infection of soybean leaves. By transiently expressing these effectors in <i>Nicotiana benthamiana</i>, we identified PsCAP1, a novel type of secreted CAP protein that triggers immune responses in multiple solanaceous plants including <i>N. benthamiana</i>. This secreted CAP protein is conserved among oomycetes, and multiple PsCAP1 homologs can be recognized by <i>N. benthamiana</i>.</li>\u0000 \u0000 <li>PsCAP1-triggered immune responses depend on the N-terminal immunogenic fragment (aa 27–151). Pretreatment of <i>N. benthamiana</i> with PsCAP1 or the immunogenic fragment increases plant resistance against <i>Phytophthora</i>. The recognition of PsCAP1 and different homologs requires the leucine-rich repeat receptor-like protein RCAP1, which associates with two central receptor-like kinases BRI1-associated receptor kinase 1 (BAK1) and suppressor of BIR1-1 (SOBIR1) <i>in planta</i>.</li>\u0000 \u0000 <li>These findings suggest that the CAP-type apoplastic effectors act as an important player in plant–microbe interactions that can be perceived by plant membrane-localized receptor to activate plant resistance.</li>\u0000 </ul>\u0000 </div>","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":"240 2","pages":"784-801"},"PeriodicalIF":9.4,"publicationDate":"2023-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41081828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jacob S. Francis, Tobias G. Mueller, Rachel L. Vannette
Epiphytic microbes frequently affect plant phenotype and fitness, but their effects depend on microbe abundance and community composition. Filtering by plant traits and deterministic dispersal-mediated processes can affect microbiome assembly, yet their relative contribution to predictable variation in microbiome is poorly understood.
We compared the effects of host-plant filtering and dispersal on nectar microbiome presence, abundance, and composition. We inoculated representative bacteria and yeast into 30 plants across four phenotypically distinct cultivars of Epilobium canum. We compared the growth of inoculated communities to openly visited flowers from a subset of the same plants.
There was clear evidence of host selection when we inoculated flowers with synthetic communities. However, plants with the highest microbial densities when inoculated did not have the highest microbial densities when openly visited. Instead, plants predictably varied in the presence of bacteria, which was correlated with pollen receipt and floral traits, suggesting a role for deterministic dispersal.
These findings suggest that host filtering could drive plant microbiome assembly in tissues where species pools are large and dispersal is high. However, deterministic differences in microbial dispersal to hosts may be equally or more important when microbes rely on an animal vector, dispersal is low, or arrival order is important.
{"title":"Intraspecific variation in realized dispersal probability and host quality shape nectar microbiomes","authors":"Jacob S. Francis, Tobias G. Mueller, Rachel L. Vannette","doi":"10.1111/nph.19195","DOIUrl":"https://doi.org/10.1111/nph.19195","url":null,"abstract":"<div>\u0000 \u0000 <p>\u0000 \u0000 </p><ul>\u0000 \u0000 \u0000 <li>Epiphytic microbes frequently affect plant phenotype and fitness, but their effects depend on microbe abundance and community composition. Filtering by plant traits and deterministic dispersal-mediated processes can affect microbiome assembly, yet their relative contribution to predictable variation in microbiome is poorly understood.</li>\u0000 \u0000 \u0000 <li>We compared the effects of host-plant filtering and dispersal on nectar microbiome presence, abundance, and composition. We inoculated representative bacteria and yeast into 30 plants across four phenotypically distinct cultivars of <i>Epilobium canum</i>. We compared the growth of inoculated communities to openly visited flowers from a subset of the same plants.</li>\u0000 \u0000 \u0000 <li>There was clear evidence of host selection when we inoculated flowers with synthetic communities. However, plants with the highest microbial densities when inoculated did not have the highest microbial densities when openly visited. Instead, plants predictably varied in the presence of bacteria, which was correlated with pollen receipt and floral traits, suggesting a role for deterministic dispersal.</li>\u0000 \u0000 \u0000 <li>These findings suggest that host filtering could drive plant microbiome assembly in tissues where species pools are large and dispersal is high. However, deterministic differences in microbial dispersal to hosts may be equally or more important when microbes rely on an animal vector, dispersal is low, or arrival order is important.</li>\u0000 </ul>\u0000 \u0000 </div>","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":"240 3","pages":"1233-1245"},"PeriodicalIF":9.4,"publicationDate":"2023-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41087758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The regulatory framework of leaf senescence is gradually becoming clearer; however, the fine regulation of this process remains largely unknown.
Here, genetic analysis revealed that U2 small nuclear ribonucleoprotein B (U2B″), a component of the spliceosome, is a negative regulator of leaf senescence. Mutation of U2B″ led to precocious leaf senescence, whereas overexpression of U2B″ extended leaf longevity. Transcriptome analysis revealed that the jasmonic acid (JA) signaling pathway was activated in the u2b″ mutant. U2B″ enhances the generation of splicing variant JASMONATE ZIM-DOMAIN 9β (JAZ9β) with an intron retention in the Jas motif, which compromises its interaction with CORONATINE INSENSITIVE1 and thus enhances the stability of JAZ9β protein. Moreover, JAZ9β could interact with MYC2 and obstruct its activity, thereby attenuating JA signaling. Correspondingly, overexpression of JAZ9β rescued the early senescence phenotype of the u2b″ mutant.
Furthermore, JA treatment promoted expression of U2B″ that was found to be a direct target of MYC2. Overexpression of MYC2 in the u2b″ mutant resulted in a more pronounced premature senescence than that in wild-type plants.
Collectively, our findings reveal that the spliceosomal protein U2B″ fine-tunes leaf senescence by enhancing the expression of JAZ9β and thereby attenuating JA signaling.
{"title":"Spliceosomal protein U2B″ delays leaf senescence by enhancing splicing variant JAZ9β expression to attenuate jasmonate signaling in Arabidopsis","authors":"Qi Yang, Shuya Tan, Hou-Ling Wang, Ting Wang, Jie Cao, Hairong Liu, Yueqi Sha, Yaning Zhao, Xinli Xia, Hongwei Guo, Zhonghai Li","doi":"10.1111/nph.19198","DOIUrl":"https://doi.org/10.1111/nph.19198","url":null,"abstract":"<div>\u0000 \u0000 <p>\u0000 \u0000 </p><ul>\u0000 \u0000 \u0000 <li>\u0000 \u0000 <p>The regulatory framework of leaf senescence is gradually becoming clearer; however, the fine regulation of this process remains largely unknown.</p>\u0000 </li>\u0000 \u0000 \u0000 <li>\u0000 \u0000 <p>Here, genetic analysis revealed that U2 small nuclear ribonucleoprotein B (U2B″), a component of the spliceosome, is a negative regulator of leaf senescence. Mutation of U2B″ led to precocious leaf senescence, whereas overexpression of <i>U2B″</i> extended leaf longevity. Transcriptome analysis revealed that the jasmonic acid (JA) signaling pathway was activated in the <i>u2b″</i> mutant. U2B″ enhances the generation of splicing variant JASMONATE ZIM-DOMAIN 9β (JAZ9β) with an intron retention in the Jas motif, which compromises its interaction with CORONATINE INSENSITIVE1 and thus enhances the stability of JAZ9β protein. Moreover, JAZ9β could interact with MYC2 and obstruct its activity, thereby attenuating JA signaling. Correspondingly, overexpression of <i>JAZ9β</i> rescued the early senescence phenotype of the <i>u2b″</i> mutant.</p>\u0000 </li>\u0000 \u0000 \u0000 <li>\u0000 \u0000 <p>Furthermore, JA treatment promoted expression of U2B″ that was found to be a direct target of MYC2. Overexpression of <i>MYC2</i> in the <i>u2b″</i> mutant resulted in a more pronounced premature senescence than that in wild-type plants.</p>\u0000 </li>\u0000 \u0000 \u0000 <li>\u0000 \u0000 <p>Collectively, our findings reveal that the spliceosomal protein U2B″ fine-tunes leaf senescence by enhancing the expression of JAZ9β and thereby attenuating JA signaling.</p>\u0000 </li>\u0000 </ul>\u0000 \u0000 </div>","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":"240 3","pages":"1116-1133"},"PeriodicalIF":9.4,"publicationDate":"2023-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41087606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sphingolipids are cell membrane components and signaling molecules that induce endoplasmic reticulum (ER) stress responses, but the underlying mechanism is unknown. Orosomucoid proteins (ORMs) negatively regulate serine palmitoyltransferase activity, thus helping maintain proper sphingolipid levels in humans, yeast, and plants.
In this report, we explored the roles of ORMs in regulating ER stress in Arabidopsis thaliana.
Loss of ORM1 and ORM2 function caused constitutive activation of the unfolded protein response (UPR), as did treatment with the ceramide synthase inhibitor Fumonisin B1 (FB1) or ceramides. FB1 treatment induced the transcription factor bZIP28 to relocate from the ER membrane to the nucleus. The transcription factor WRKY75 positively regulates the UPR and physically interacted with bZIP28. We also found that the orm mutants showed impaired ER-associated degradation (ERAD), blocking the degradation of misfolded MILDEW RESISTANCE LOCUS-O 12 (MLO-12). ORM1 and ORM2 bind to EMS-MUTAGENIZED BRI1 SUPPRESSOR 7 (EBS7), a plant-specific component of the Arabidopsis ERAD complex, and regulate its stability. These data strongly suggest that ORMs in the ER membrane play vital roles in the UPR and ERAD pathways to prevent ER stress in Arabidopsis.
Our results reveal that ORMs coordinate sphingolipid homeostasis with ER quality control and play a role in stress responses.
{"title":"Orosomucoid proteins limit endoplasmic reticulum stress in plants","authors":"Ling-Yan Wang, Jian Li, Benqiang Gong, Rui-Hua Wang, Yi-Li Chen, Jian Yin, Chang Yang, Jia-Ting Lin, Hao-Zhuo Liu, Yubing Yang, Jianfeng Li, Chunyu Li, Nan Yao","doi":"10.1111/nph.19200","DOIUrl":"https://doi.org/10.1111/nph.19200","url":null,"abstract":"<div>\u0000 \u0000 <p>\u0000 \u0000 </p><ul>\u0000 \u0000 \u0000 <li>Sphingolipids are cell membrane components and signaling molecules that induce endoplasmic reticulum (ER) stress responses, but the underlying mechanism is unknown. Orosomucoid proteins (ORMs) negatively regulate serine palmitoyltransferase activity, thus helping maintain proper sphingolipid levels in humans, yeast, and plants.</li>\u0000 \u0000 \u0000 <li>In this report, we explored the roles of ORMs in regulating ER stress in <i>Arabidopsis thaliana</i>.</li>\u0000 \u0000 \u0000 <li>Loss of ORM1 and ORM2 function caused constitutive activation of the unfolded protein response (UPR), as did treatment with the ceramide synthase inhibitor Fumonisin B1 (FB1) or ceramides. FB1 treatment induced the transcription factor bZIP28 to relocate from the ER membrane to the nucleus. The transcription factor WRKY75 positively regulates the UPR and physically interacted with bZIP28. We also found that the <i>orm</i> mutants showed impaired ER-associated degradation (ERAD), blocking the degradation of misfolded MILDEW RESISTANCE LOCUS-O 12 (MLO-12). ORM1 and ORM2 bind to EMS-MUTAGENIZED BRI1 SUPPRESSOR 7 (EBS7), a plant-specific component of the Arabidopsis ERAD complex, and regulate its stability. These data strongly suggest that ORMs in the ER membrane play vital roles in the UPR and ERAD pathways to prevent ER stress in Arabidopsis.</li>\u0000 \u0000 \u0000 <li>Our results reveal that ORMs coordinate sphingolipid homeostasis with ER quality control and play a role in stress responses.</li>\u0000 </ul>\u0000 \u0000 </div>","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":"240 3","pages":"1134-1148"},"PeriodicalIF":9.4,"publicationDate":"2023-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41087603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Youmei Huang, Liping Liu, Mengnan Chai, Han Su, Suzhuo Ma, Kaichuang Liu, Yaru Tian, Zhuangyuan Cao, Xinpeng Xi, Wenhui Zhu, Jingang Qi, Ravishankar Palanivelu, Yuan Qin, Hanyang Cai
Germline development is a key step in sexual reproduction. Sexual plant reproduction begins with the formation of haploid spores by meiosis of megaspore mother cells (MMCs). Although many evidences, directly or indirectly, show that epigenetics plays an important role in MMC specification, how it controls the commitment of the MMC to downstream stages of germline development is still unclear.
Electrophoretic mobility shift assay (EMSA), western blot, immunofluorescence, and chromatin immunoprecipitation coupled with quantitative PCR analyses were performed. Genetic interactions between BZR1 transcription factor family and the SWR1-SDG2-ER pathway in the control of female germline development were further studied.
The present findings showed in Arabidopsis that two epigenetic factors, the chromatin remodeling complex SWI2/SNF2-RELATED 1 (SWR1) and a writer for H3K4me3 histone modification SET DOMAIN GROUP 2 (SDG2), genetically interact with the ERECTA (ER) receptor kinase signaling pathway and regulate female germline development by restricting the MMC cell fate to a single cell in the ovule primordium and ensure that only that single cell undergoes meiosis and subsequent megaspore degeneration. We also showed that SWR1-SDG2-ER signaling module regulates female germline development by promoting the protein accumulation of BZR1 transcription factor family on the promoters of primary miRNA processing factors, HYPONASTIC LEAVES 1 (HYL1), DICER-LIKE 1 (DCL1), and SERRATE (SE) to activate their expression.
Our study elucidated a Gene Regulation Network that provides new insights for understanding how epigenetic factors and receptor kinase signaling pathways function in concert to control female germline development in Arabidopsis.
生殖系发育是有性生殖的关键步骤。植物有性繁殖始于大孢子母细胞减数分裂形成单倍体孢子。尽管许多证据直接或间接表明表观遗传学在MMC规范中发挥着重要作用，但它如何控制MMC对种系发育下游阶段的承诺仍不清楚。进行电泳迁移率转移分析（EMSA）、蛋白质印迹、免疫荧光和染色质免疫沉淀结合定量PCR分析。进一步研究了BZR1转录因子家族与SWR1-SDG2-ER通路在控制雌性生殖系发育中的遗传相互作用。目前的研究结果表明，在拟南芥中，两种表观遗传因子——染色质重塑复合物SWI2/SNF2-RATED 1（SWR1）和H3K4me3组蛋白修饰SET DOMAIN GROUP 2（SDG2）的作者，与ERECTA（ER）受体激酶信号通路遗传相互作用，并通过将MMC细胞命运限制在胚珠原基中的单个细胞来调节雌性生殖系发育，并确保只有该单个细胞经历减数分裂和随后的大孢子变性。我们还发现，SWR1-SDG2-ER信号模块通过促进BZR1转录因子家族在初级miRNA处理因子、缺氧叶1（HYL1）、二细胞样1（DCL1）和SERRATE（SE）启动子上的蛋白质积累来调节雌性生殖系发育，以激活其表达。我们的研究阐明了一个基因调控网络，该网络为理解表观遗传因子和受体激酶信号通路如何协同控制拟南芥雌性生殖系发育提供了新的见解。
{"title":"Epigenetic regulation of female germline development through ERECTA signaling pathway","authors":"Youmei Huang, Liping Liu, Mengnan Chai, Han Su, Suzhuo Ma, Kaichuang Liu, Yaru Tian, Zhuangyuan Cao, Xinpeng Xi, Wenhui Zhu, Jingang Qi, Ravishankar Palanivelu, Yuan Qin, Hanyang Cai","doi":"10.1111/nph.19217","DOIUrl":"https://doi.org/10.1111/nph.19217","url":null,"abstract":"<div>\u0000 \u0000 <p>\u0000 \u0000 </p><ul>\u0000 \u0000 \u0000 <li>Germline development is a key step in sexual reproduction. Sexual plant reproduction begins with the formation of haploid spores by meiosis of megaspore mother cells (MMCs). Although many evidences, directly or indirectly, show that epigenetics plays an important role in MMC specification, how it controls the commitment of the MMC to downstream stages of germline development is still unclear.</li>\u0000 \u0000 \u0000 <li>Electrophoretic mobility shift assay (EMSA), western blot, immunofluorescence, and chromatin immunoprecipitation coupled with quantitative PCR analyses were performed. Genetic interactions between BZR1 transcription factor family and the SWR1-SDG2-ER pathway in the control of female germline development were further studied.</li>\u0000 \u0000 \u0000 <li>The present findings showed in Arabidopsis that two epigenetic factors, the chromatin remodeling complex SWI2/SNF2-RELATED 1 (SWR1) and a writer for H3K4me3 histone modification SET DOMAIN GROUP 2 (SDG2), genetically interact with the ERECTA (ER) receptor kinase signaling pathway and regulate female germline development by restricting the MMC cell fate to a single cell in the ovule primordium and ensure that only that single cell undergoes meiosis and subsequent megaspore degeneration. We also showed that SWR1-SDG2-ER signaling module regulates female germline development by promoting the protein accumulation of BZR1 transcription factor family on the promoters of primary miRNA processing factors, <i>HYPONASTIC LEAVES 1</i> (<i>HYL1</i>), <i>DICER-LIKE 1</i> (<i>DCL1</i>), and <i>SERRATE</i> (<i>SE</i>) to activate their expression.</li>\u0000 \u0000 \u0000 <li>Our study elucidated a Gene Regulation Network that provides new insights for understanding how epigenetic factors and receptor kinase signaling pathways function in concert to control female germline development in Arabidopsis.</li>\u0000 </ul>\u0000 \u0000 </div>","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":"240 3","pages":"1015-1033"},"PeriodicalIF":9.4,"publicationDate":"2023-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41087608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Zhigang Liao, Yunchao Zhang, Qing Yu, Weicong Fang, Meiyao Chen, Tianfei Li, Yi Liu, Zaochang Liu, Liang Chen, Shunwu Yu, Hui Xia, Hong-Wei Xue, Hong Yu, Lijun Luo
The drought caused by global warming seriously affects the crop growth and agricultural production. Plants have evolved distinct strategies to cope with the drought environment. Under drought stress, energy and resources should be diverted from growth toward stress management.
However, the molecular mechanism underlying coordination of growth and drought response remains largely elusive.
Here, we discovered that most of the gibberellin (GA) metabolic genes were regulated by water scarcity in rice, leading to the lower GA contents and hence inhibited plant growth. Low GA contents resulted in the accumulation of more GA signaling negative regulator SLENDER RICE 1, which inhibited the degradation of abscisic acid (ABA) receptor PYL10 by competitively binding to the co-activator of anaphase-promoting complex TAD1, resulting in the enhanced ABA response and drought tolerance.
These results elucidate the synergistic regulation of crop growth inhibition and promotion of drought tolerance and survival, and provide useful genetic resource in breeding improvement of crop drought resistance.
{"title":"Coordination of growth and drought responses by GA-ABA signaling in rice","authors":"Zhigang Liao, Yunchao Zhang, Qing Yu, Weicong Fang, Meiyao Chen, Tianfei Li, Yi Liu, Zaochang Liu, Liang Chen, Shunwu Yu, Hui Xia, Hong-Wei Xue, Hong Yu, Lijun Luo","doi":"10.1111/nph.19209","DOIUrl":"https://doi.org/10.1111/nph.19209","url":null,"abstract":"<div>\u0000 \u0000 <p>\u0000 \u0000 </p><ul>\u0000 \u0000 \u0000 <li>The drought caused by global warming seriously affects the crop growth and agricultural production. Plants have evolved distinct strategies to cope with the drought environment. Under drought stress, energy and resources should be diverted from growth toward stress management.</li>\u0000 \u0000 \u0000 <li>However, the molecular mechanism underlying coordination of growth and drought response remains largely elusive.</li>\u0000 \u0000 \u0000 <li>Here, we discovered that most of the gibberellin (GA) metabolic genes were regulated by water scarcity in rice, leading to the lower GA contents and hence inhibited plant growth. Low GA contents resulted in the accumulation of more GA signaling negative regulator SLENDER RICE 1, which inhibited the degradation of abscisic acid (ABA) receptor PYL10 by competitively binding to the co-activator of anaphase-promoting complex TAD1, resulting in the enhanced ABA response and drought tolerance.</li>\u0000 \u0000 \u0000 <li>These results elucidate the synergistic regulation of crop growth inhibition and promotion of drought tolerance and survival, and provide useful genetic resource in breeding improvement of crop drought resistance.</li>\u0000 </ul>\u0000 \u0000 </div>","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":"240 3","pages":"1149-1161"},"PeriodicalIF":9.4,"publicationDate":"2023-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41087546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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