A limited number of studies using static spatial approaches have found that built environment variables are associated with bike share use and fewer have used spatially dynamic activity spaces to examine these relationships. The aim of this pilot study was to examine associations between built environment characteristics of daily activity spaces and bike share using three different geographic information system methods. Thirty-two adult members of Boston’s Blue Bikes bike share wore a GPS unit for up to 7 days. GPS points were used to create buffered track, minimum convex hull (MCH), and standard deviational ellipse (SDE) activity spaces. Multilevel logistic regression was used to estimate associations between docking station density, overall bicycle network density, shared-use trail density, intersection density, land use mix, and greenness, with bike share use. Bike share station density within SDE activity spaces showed a significant positive association with bike share (odds ratio (OR) = 1.19; 95 % confidence interval (CI): 1.02, 1.39). Total bike network and shared-use trail densities within MCH activity spaces were positively associated with bike share (OR = 1.13; 95 % CI: 1.02, 1.26 and OR = 1.75; 95 % CI: 1.06, 2.89, respectively). Intersection density within SDE activity spaces was inversely associated with bike share (OR = 0.91; 95 % CI: 0.83, 0.99). GPS tracking of individuals allowed for spatially and temporally dynamic identification of environmental exposures potentially relevant to bike share use. Overall, the findings are consistent with prior research on the environmental correlates of bike share and reinforce the importance of bicycle infrastructure to support greater bike share use. At the same time larger studies are needed to explore optimal geographic methods to define activity spaces in relation to bike share.