Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology最新文献

{"title":"The influence of triiodothyronine on the immune response and extracellular matrix remodeling during zebrafish heart regeneration","authors":"Reece R.B. Long ,&nbsp;Oliver M.N. Bullingham ,&nbsp;Benjamin Baylis ,&nbsp;Jared B. Shaftoe ,&nbsp;John R. Dutcher ,&nbsp;Todd E. Gillis","doi":"10.1016/j.cbpa.2024.111769","DOIUrl":"10.1016/j.cbpa.2024.111769","url":null,"abstract":"<div><div>Damage to the human heart is an irreparable process that results in a permanent impairment in cardiac function. There are, however, a number of vertebrate species including zebrafish (<em>Danio rerio</em>) that can regenerate their hearts following significant injury. In contrast to these regenerative species, mammals are known to have high levels of thyroid hormones, which has been proposed to play a role in this difference in regenerative capacity. However, the mechanisms through which thyroid hormones effect heart regeneration are not fully understood. Here, zebrafish were exposed to exogenous triiodothyronine (T₃) for two weeks and then their hearts were damaged through cryoinjury to investigate the effect of thyroid hormones on ECM remodeling and the components of the immune response during heart regeneration. Additionally, cardiac fibroblasts derived from trout, another species of fish known to display cardiac regenerative capacity, were exposed to T₃ <em>in vitro</em> to analyze any direct effects of T₃ on collagen deposition. It was found that cryoinjury induction results in an increase in myocardial stiffness, but this response was muted in T₃ exposed zebrafish. The measurement of relevant marker gene transcripts suggests that T₃ exposure reduces the recruitment of macrophages to the damaged zebrafish heart immediately following injury but had no effect on the regulation of collagen deposition by cultured trout fibroblasts. These results suggest that T₃ effects both the immune response and ECM remodeling in zebrafish following cardiac injury.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"299 ","pages":"Article 111769"},"PeriodicalIF":2.1,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142553801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physiological responses of euryhaline marine fish to naturally-occurring hypersalinity","authors":"Andrew J. Esbaugh","doi":"10.1016/j.cbpa.2024.111768","DOIUrl":"10.1016/j.cbpa.2024.111768","url":null,"abstract":"<div><div>Hypersaline habitats are generally defined as those with salinities in excess of 40 ppt. Well-known hypersaline regions (e.g. salt and soda lakes) have a well-earned reputation for being among the most inhospitable habitats in the world, and fish endemic to these areas have been the subject of much research related to extremophile physiology. Yet, marine coastal hypersalinity is both a common occurrence and a growing consideration in many marine coastal ecosystems, in part owing to human influence (e.g. evaporation, river diversion, desalination effluent). Importantly, any increase in salinity will elevate the osmoregulatory challenges experienced by a fish, which must be overcome by increasing the capacity to imbibe and absorb water and excrete ions. While great attention has been given to dynamic osmoregulatory processes with respect to freshwater to seawater transitions, and to the extreme hypersalinity tolerance that is associated with the adoption of an osmo-conforming strategy, relatively little focus has been placed on the physiological implications of moderate hypersalinity exposures (e.g. ≤ 60 ppt). Importantly, these exposures often represent the threshold of osmoregulatory performance owing to energetic constraints on ion excretion and efficiency limitations on water absorption. This review will explore the current state of knowledge with respect to hypersalinity exposure in euryhaline fishes, while placing a particular focus on the physiological constraints, plasticity and downstream implications of long-term exposure to moderate hypersalinity.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"299 ","pages":"Article 111768"},"PeriodicalIF":2.1,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142513347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reproductive effort affects cellular response in the mantle of Nodipecten subnodosus scallops exposed to acute hyperthermia","authors":"A. Marzari ,&nbsp;I.S. Racotta ,&nbsp;C. Escobedo-Fregoso ,&nbsp;S. Artigaud ,&nbsp;E. Kraffe ,&nbsp;R.L. Salgado-García","doi":"10.1016/j.cbpa.2024.111766","DOIUrl":"10.1016/j.cbpa.2024.111766","url":null,"abstract":"<div><div>In marine ecosystems, temperature regulates the energy metabolism of animals. In the last decades, the temperature increase was related to mass mortality events of marine ectotherms, particularly during high-energy investment for reproduction. In scallops, the mantle has been poorly investigated while this tissue covers more than 40 % of the body mass, contributing to the perception of surrounding environmental stimuli. Our aim was to assess the cellular and molecular responses linked to energy metabolism in the mantle of adult <em>N. subnodosus</em> facing acute hyperthermia during reproductive effort. Scallops collected in spring (late gametogenesis) and summer (ripe gonads) were exposed to a control temperature (22 °C) or acute hyperthermia (30 °C) for 24 h. In spring, increased arginine kinase (AK) activity together with increased pyruvate kinase/citrate synthase ratio (PK/CS) suggested an enhanced carbohydrate, pyruvate, and arginine metabolism to maintain the adenylate energy charge (AEC) in the mantle of scallops coping with acute thermal increase. In summer, animals decreased their AEC (5 %) and arginine phosphate pool (40 %) and increased their anaerobic metabolism as shown by enhanced activities of lactate-dehydrogenase (LDH) and octopine dehydrogenase (ODH), respectively. The abundance of twenty proteins involved in energy metabolism (isocitrate dehydrogenase, ATP synthase subunit β), protein protection (cognate heat shock protein 70), and cytoskeleton (actins and tubulins) were affected only by season. These results underlie the role of the mantle of <em>N. subnodosus</em> in the seasonal responses of this tissue to thermal fluctuations during reproductive effort with possible implications for the physiological performance of scallops under heat waves in wild or harvest conditions.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"299 ","pages":"Article 111766"},"PeriodicalIF":2.1,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142481386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physiological adaptations of red blood cells during aestivation in the south American lungfish Lepidosiren paradoxa","authors":"Elisa M. Fonseca ,&nbsp;Sofia L.B. da S. Matos ,&nbsp;Alan E. Piedade ,&nbsp;Flávio A.G. Mourão ,&nbsp;Kenia C. Bícego ,&nbsp;Luciane H. Gargaglioni ,&nbsp;Glauber S.F. da Silva","doi":"10.1016/j.cbpa.2024.111765","DOIUrl":"10.1016/j.cbpa.2024.111765","url":null,"abstract":"<div><div>The South American lungfish <em>Lepidosiren paradoxa</em> inhabits areas with variable pluvial regimes. During aestivation (dormancy state observed in some species during dry seasons), the prolonged period of dryness imposes osmotic stress. We aimed to investigate the physiological and morphological adaptations of RBCs in <em>Lepidosiren paradoxa</em> during aestivation. Here, the lungfish were subjected to aestivation for 20 and 40 days and compared to a control group in an active period. The osmotic fragility, blood osmolality, and pH were measured. Blood smears were performed to assess morphological changes in the RBCs. <em>Lepidosiren paradoxa</em> presented lower hemolysis when compared with a teleost fish and tegu lizard. Hemolysis increased when the lungfish was subjected to 40 days of aestivation (from 6.04 % to 16.51 %; control vs 40-day aestivation). Hematocrit rose in both aestivation groups compared to the control (26.36 %, 41.36 % and 41 %, control, 20 and 40 days, respectively; <em>p</em> &lt; 0.05) indicating changes in RBC volume and hydration status. Moreover, the results revealed increased osmolality in the 40-day aestivation group (244.4 vs 372.1 mmol/Kg; control vs 40-day aestivation). 40 days of aestivation led to a decrease in blood pH when compared to the control and 20-day aestivation groups. Both aestivation durations resulted in a reduction in the perimeter and cell diameters in at least one direction of the RBCs (24 % mean reduction in size from control to 40 days aestivation). These findings suggest that South American lungfish possess remarkable physiological and morphological adaptations in their RBCs during aestivation.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"299 ","pages":"Article 111765"},"PeriodicalIF":2.1,"publicationDate":"2024-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142441406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization, expression, and polymorphism of MHC II α and MHC II β in Sichuan taimen (Hucho bleekeri)","authors":"Yeyu Chen ,&nbsp;Xiaoyun Wu ,&nbsp;Huanchao Yang ,&nbsp;Zhao Liu ,&nbsp;Yanling Chen ,&nbsp;Qinyao Wei ,&nbsp;Jue Lin ,&nbsp;Yi Yu ,&nbsp;Quanyu Tu ,&nbsp;Hua Li","doi":"10.1016/j.cbpa.2024.111767","DOIUrl":"10.1016/j.cbpa.2024.111767","url":null,"abstract":"<div><div>The major histocompatibility complex (MHC) is involved in antigen presentation and plays an essential role in regulating immune function. In the present study, we identified two MHC class II genes and investigated their potential roles in <em>Hucho bleekeri</em>. The MHC II α and MHC II β of <em>H. bleekeri</em> had typical leading peptides, extracellular domains, connecting peptides, transmembrane region, and cytoplasmic region. Amino acid sequence comparison revealed that MHC II of <em>H. bleekeri</em> has high homology with other vertebrates, among which homology with salmonid fish was the highest. Phylogenetic analysis showed that <em>H. bleekeri</em> MHC II clustered with salmonid fish; moreover they clustered with orthologous genes of other fish, whereas mammalian MHC II clustered into a separate branch. Tissue distribution analysis revealed <em>MHC II</em> was widely expressed in all tested tissues, with both <em>MHC II α</em> and <em>MHC II β</em> highly expressed in the spleen, gill, kidney, and hindgut. After lipopolysaccharide (LPS) and polyinosinic:polycytidylic acid (poly(I:C)) stimulation, the expression of <em>MHC II</em> in the head kidney and spleen of <em>H. bleekeri</em> was significantly upregulated. Compared with <em>MHC II α</em>, <em>MHC II β</em> acted faster in response to the stimulation. Polymorphism analysis of <em>MHC II</em> revealed that all the different alleles belonged to the same major type, and very limited polymorphisms were found in <em>H. bleekeri MHC II α</em> and <em>II β</em>. Selection pressure analysis showed signs of weak and non-significant positive selection in the <em>MHC II α</em> and <em>MHC II β</em> extracellular region. Our study reveals the potential role of MHC II in the immune response of <em>H. bleekeri</em> and provides a reference for studying the evolutionary model of teleost MHC II.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"299 ","pages":"Article 111767"},"PeriodicalIF":2.1,"publicationDate":"2024-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142441383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cogrowth advantage: Intestinal microbiota analysis of Bufo gargarizans and Rana chensinensis","authors":"Kaiyue Li ,&nbsp;Jiayi Li ,&nbsp;Shuangyan Luo ,&nbsp;Lihong Chai","doi":"10.1016/j.cbpa.2024.111764","DOIUrl":"10.1016/j.cbpa.2024.111764","url":null,"abstract":"<div><div>Intestinal microbiota has profound effects on host health and adaptation to environmental changes. <em>Bufo gargarizans</em> and <em>Rana chensinensis</em> coexist in the same habitat and have been paid much attention to economically because of their medicinal value. To date, no comparison of differences between single and mixed populations has been made. In our study, differences in the structure and function of the intestinal microbial of <em>B. gargarizans</em> and <em>R. chensinensis</em> in environments of single-species and mixed-species growth were investigated by high-throughput sequencing. Our results suggest that the cogrowth of <em>B. gargarizans</em> and <em>R. chensinensis</em> could lead to the decrease of the abundance of pathogenic bacteria (<em>Bosea</em>) and the introduction or increase of beneficial bacteria (<em>Kaistia</em>, <em>Cetobacterium</em> and <em>Erysipelatoclostridium</em>). The Tax4Fun-based functional predictions revealed that the level of pathways involved in the metabolism of <em>R. chensinensis</em> in mixed-species aquaria is greatly up-regulated. This study provides useful information for ecologists, ecosystem policy makers and wildlife conservationists to promote more effective conservation measures.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"299 ","pages":"Article 111764"},"PeriodicalIF":2.1,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142441385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring immunoglobulin A as a stress biomarker in lions (Panthera leo): Validation of an immunoassay for its measurement in feces","authors":"Paula Serres-Corral ,&nbsp;Sergi Olvera-Maneu ,&nbsp;Vanessa Almagro ,&nbsp;Loles Carbonell ,&nbsp;Santiago Borragán ,&nbsp;Eva Martínez-Nevado ,&nbsp;Miguel Angel Quevedo ,&nbsp;Hugo Fernández-Bellon ,&nbsp;Annaïs Carbajal ,&nbsp;Manel López-Béjar","doi":"10.1016/j.cbpa.2024.111762","DOIUrl":"10.1016/j.cbpa.2024.111762","url":null,"abstract":"<div><div>Immunoglobulin A (IgA) has been investigated as a stress biomarker with the potential to complement glucocorticoid measurements in welfare assessments. This study aimed to develop the methodology and validate an enzyme immunoassay (EIA) for quantifying IgA in feces (FIgA) of lions (<em>Panthera leo</em>), investigate excretion patterns of FIgA under baseline conditions in captive lions, and explore its relationship with fecal glucocorticoid metabolites (FGM). Feces were collected from 11 lions housed in stable social groups at four Spanish zoos over a period of two to six weeks. FIgA was reliably quantified using a commercial EIA, with concentrations ranging from 0.28 to 794.17 μg IgA/g feces, showing substantial intra- and inter-individual variability. Females had significantly higher FIgA concentrations than males (113.10 vs 54.96 μg IgA/g feces; <em>p</em> &lt; 0.01). Additionally, FIgA concentrations varied across zoos (<em>p</em> &lt; 0.001). Positive correlations were found between FIgA and FGM for all samples combined (rho = 0.43, <em>p</em> &lt; 0.001) and across individual means (rho = 0.70, <em>p</em> &lt; 0.05), but not consistently when examining each lion separately. This study demonstrates for the first time that IgA can be reliably quantified in lion feces, paving the way for its application in welfare studies.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"299 ","pages":"Article 111762"},"PeriodicalIF":2.1,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142441384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Binding site redundancy is critical for the regulation of fas by miR-30c in blunt snout bream (Megalobrama amblycephala)","authors":"Xiaoyan Jia,&nbsp;Jie Liu,&nbsp;Weibo Jiang,&nbsp;Le Chang,&nbsp;Xiaoxue Shen,&nbsp;Guangzhen Jiang,&nbsp;Xiangfei Li,&nbsp;Cheng Chi,&nbsp;Wenbin Liu,&nbsp;Dingdong Zhang","doi":"10.1016/j.cbpa.2024.111763","DOIUrl":"10.1016/j.cbpa.2024.111763","url":null,"abstract":"<div><div>MiR-30c and fatty acid synthase (<em>fas</em>) both play important roles in physiological processes such as lipid synthesis and fat metabolism. Predictive analysis revealed that <em>fas</em> is a target gene of miR-30c with multiple seed sites. Seed sites are useful to predict miRNA targeting relationships; however, detailed analyses of seed sites in fish genomes remain poorly studied. In this study, the regulatory relationship between miR-30c and <em>fas,</em> number and effect of seed regions, and mechanism by which miR-30c regulates lipid metabolism were evaluated in blunt snout bream (<em>Megalobrama amblycephala</em>). Four miR-30c target sites for <em>fas</em> were identified using various prediction tools. miR-30c mimics were transfected into 293 T cells, and dual-luciferase reporter assays were used to evaluate the roles of different <em>fas</em> target sites. When a single target site was mutated, relative luciferase activity was higher than that in the control group, with different activity levels depending on the mutation site. When multiple target sites were mutated, relative luciferase activity increased significantly as the number of mutation sites increased and was the highest when the four sites were mutated simultaneously. The miR-30c agomir was injected into the abdominal cavity of <em>M. amblycephala</em> at various concentrations for analyses of physiological and biochemical parameters in the liver and blood and the expression of genes related to lipid metabolism in the liver. Total cholesterol, free fatty acid, triglyceride, and low density lipoprotein levels were significantly lower after miR-30c agomir injection comparing to the control (<em>P</em> &lt; 0.05). Additionally, the expression levels of genes related to lipid metabolism were significantly lower after miR-30c agomir injection than in the control (<em>P</em> &lt; 0.05). In summary, this study identified four specific miR-30c target sites in the 3′ UTR of <em>fas</em> mRNA; the effects of these sites are cumulative, and the redundancy ensures the accurate regulation of <em>fas</em> during evolution. In addition, miR-30c has a negative regulatory effect on <em>fas</em> and regulates lipid metabolism via various genes related to this process. Therefore, the regulation of miR-30c can effectively ameliorate the side effects of a high-fat diet on liver function in <em>M. amblycephala</em>.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"299 ","pages":"Article 111763"},"PeriodicalIF":2.1,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142441405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High temperature and hyperkalemia increase vulnerability of navaga cod (Eleginus nawaga) cardiomyocytes to the ecotoxicant 3-methyl-phenanthrene","authors":"Denis V. Abramochkin ,&nbsp;Artem Shamshura ,&nbsp;Irina Dzhumaniiazova ,&nbsp;Oksana B. Pustovit ,&nbsp;Aleksandr A. Mishchenko","doi":"10.1016/j.cbpa.2024.111761","DOIUrl":"10.1016/j.cbpa.2024.111761","url":null,"abstract":"<div><div>Oil and gas mining and transportation in the Arctic can lead to release of polycyclic aromatic hydrocarbons (PAHs) in the ocean and freshwater basins. PAHs are known for their toxic effects in fish hearts, including the inhibition of main ionic currents (I_Kr, I_Na and I_CaL) in fish cardiac myocytes. The present study is the first one to assess the effect of a particular PAH abundant in crude oil and diesel, namely 3-methyl-phenanthrene (3-MP), on the electrical excitability (EE) of cardiomyocytes from navaga cod (<em>Eleginus nawaga</em>), commercial fish species from the Arctic. Action potentials (APs) were elicited in current-clamp experiments at 9, 15 and 21 °C, and AP characteristics and the current needed to elicit APs were examined. Also, the effects of 3 μM 3-MP were tested at 3 temperatures and in normal (3.5 mM) and high (8 mM) extracellular K⁺ concentrations.</div><div>Elevation of temperature leads to hyperpolarization of resting membrane potential and AP shortening, but does not decrease EE. 3-MP was found to suppress EE in cardiomyocytes at 9 and 15 °C, but not at 21 °C. High extracellular K⁺ itself drastically decreases EE, although it does not worsen the effect of 3-MP. However, combination of hyperthermia and high K⁺ leads to augmentation of depressive effect of 3-MP on EE. We hypothesize that hyperthermia rescues Na⁺ channels from inactivation due to membrane hyperpolarization, thereby compensating for the partial inhibition of I_Na by 3-MP. However, elevation of extracellular K⁺ nullifies this protective mechanism by depolarizing the resting potential and aggravates the effect of 3-MP.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"299 ","pages":"Article 111761"},"PeriodicalIF":2.1,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142382533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lower adaptive immunity in invasive Egyptian geese compared to sympatric native waterfowls","authors":"Simone Messina ,&nbsp;Hanna Prüter ,&nbsp;Gábor Árpád Czirják ,&nbsp;David Costantini","doi":"10.1016/j.cbpa.2024.111752","DOIUrl":"10.1016/j.cbpa.2024.111752","url":null,"abstract":"<div><div>Successful invasive species increase their spreading success by trading-off nutritional and metabolic resources allocated to reproduction and range expansion with other costly body functions. One proposed mechanism for the reallocation of resources is a trade-off with the immune function and the regulation of oxidative status. Relying on a panel of blood-based markers of immune function and oxidative status quantified in an invasive species (Egyptian goose) and two native competing species (mallard and mute swan) in Germany, we tested the hypothesis that the invasive species would have (i) lower investment in immune function, (ii) lower levels of oxidative damage, and (iii) no higher antioxidant defences compared to the native species. We found lower levels of adaptive immune markers (lymphocytes and immunoglobulin Y), in the invasive species compared to the two native species. Innate immune profile was generally similar between Egyptian geese and mallards. By contrast, mute swans showed higher levels of heterophils and lysozymes, and lower levels of bacteria killing ability compared to both Egyptian geese and mallards. Mute swans also showed higher levels of haemolysis and haemagglutination, but lower levels of monocytes and haematocrit compared to Egyptian geese. Reactive oxygen metabolites, a marker of oxidative damage, were higher in mallards and lower in Egyptian geese compared to the other waterfowl species, while levels of antioxidants were generally similar among the three species. Our results point to a reduced investment in adaptive immune function in the invasive species as a possible resources-saving immunological strategy due to the loss of co-evolved parasites in the new colonised habitats, as observed in a previous study. A lower investment in immune function may benefit other energy-demanding activities, such as reproduction, dispersal, and territoriality, while maintaining relatively higher innate immunity is beneficial since invasive species mainly encounter novel pathogens. Results pointed out also other important species-specific differences in baseline immune status, supporting previous findings on the relationship between species' body mass and immune profile.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"299 ","pages":"Article 111752"},"PeriodicalIF":2.1,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142376345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}