Malaria remains a major global health challenge, with millions of cases and significant mortality each year, particularly in tropical and subtropical regions. Conventional diagnostic techniques, such as microscopy and rapid diagnostic tests (RDTs), suffer from limitations including reagent instability and false negatives. This necessitates rapid and reliable diagnostic alternatives for effective management and control. Surface Plasmon Resonance (SPR) based biosensors are now widely recognised for detecting biomolecular interactions in real time, without the need for labelling. In this work, we present the design and numerical analysis of a high-performance Kretschmann-configured surface plasmon resonance-based sensor. The proposed structure incorporates silver (Ag) as a metal layer, silicon (Si) as a dielectric layer and ultrathin TiO2 as an additional dielectric layer to enhance optical response for medical diagnostics. The sensor is evaluated for the detection of malaria-infected red blood cells across different intraerythrocytic developmental stages. Performance metrics were analysed using the finite element method (FEM) in COMSOL Multiphysics and the transfer matrix method (TMM) in MATLAB environment. Optimization of thickness and layer configuration yielded sensitivity of 425 deg/RIU, 337.03 deg/RIU, and 302.70 deg/RIU for ring, trophozoite and schizont phases, respectively. Corresponding figure of merit (FoM) was calculated as 124.70 RIU−1, 109.03 RIU−1 and 101.95 RIU−1 with a maximum detection accuracy of 0.336 deg−1 for the schizont phase. Furthermore, a tolerance study was done to assess the robustness against minor fabrication errors. These results demonstrate the potential of the suggested SPR sensor, which utilises enhanced plasmonic responses to detect minimal refractive index variations, as a platform for early malaria diagnosis.
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