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Enhancing starch hydrolysis in sweet potato (Ipomoea batatas (L.) Lam.) through CaCl2 solution mashing: insights into endogenous amylase activity 通过 CaCl2 溶液捣碎提高甘薯(Ipomoea batatas (L.) Lam.)的淀粉水解能力:对内源性淀粉酶活性的认识
IF 2.701 Pub Date : 2024-05-27 DOI: 10.1007/s13197-024-06008-x
Marina Fernanda da Silva Junges, Daniele Bach, Luiz Gustavo Lacerda, Aline Alberti, Alessandro Nogueira, Ivo Mottin Demiate

Sweet potato (Ipomoea batatas (L.) Lam.) is a tuber root crop with high economical potential and China is responsible for harvesting roughly 70% of the world production. It is relatively easy to cultivate and has high nutritional value, including fiber, vitamins, starch and sugars. Cooking the roots results in deep changes of the carbohydrate profile due to the presence of active endogenous amylases. The objective of the present study was to evaluate the influence of CaCl2 mashing at different temperature-time conditions on the starch hydrolysis by endogenous amylases of sweet potato in order to suggest potential industrial applications. Three thermal treatments were performed with the aid of a Rapid Visco Analyzer (RVA), which allowed programming temperature-time profiles. After the treatments, the sugar analysis was carried out by high performance liquid chromatography (HPLC). Total starch content was measured in the insoluble residue from the hydrolysis and the hydrolysis efficiency was calculated. The results have demonstrated that the presence of CaCl2 enhanced enzyme activity, resulting in higher sugar yields when compared to hydrolysis conducted without CaCl2. The results from the present study are promising to the use of sweet potato to produce bioethanol, maltose syrup and vinegar.

甘薯(Ipomoea batatas (L.) Lam.)是一种经济潜力巨大的块根作物,中国的产量约占世界总产量的 70%。它比较容易种植,营养价值高,包括纤维、维生素、淀粉和糖。由于存在活性内源淀粉酶,烹饪根茎会导致碳水化合物谱发生深刻变化。本研究的目的是评估在不同温度-时间条件下 CaCl2 糖化对甘薯内源淀粉酶水解淀粉的影响,从而提出潜在的工业应用建议。借助快速粘度分析仪(RVA)进行了三次热处理,该分析仪可对温度-时间曲线进行编程。处理后,采用高效液相色谱法(HPLC)进行糖分分析。在水解后的不溶残留物中测量了总淀粉含量,并计算了水解效率。结果表明,与不加 CaCl2 的水解相比,CaCl2 的存在增强了酶的活性,从而提高了糖的产量。本研究的结果有望用于利用甘薯生产生物乙醇、麦芽糖浆和醋。
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引用次数: 0
Long-term storage of pink pepper essential oil microencapsulated by chickpea protein/pectin complexes: volatile release, antioxidant and antimicrobial activities 用鹰嘴豆蛋白/pectin 复合物微胶囊长期储存粉红胡椒精油:挥发性释放、抗氧化和抗菌活性
IF 2.701 Pub Date : 2024-05-27 DOI: 10.1007/s13197-024-06007-y
Poliana Moser, Nathalie Almeida Lopes, Adilson Roberto Locali-Pereira, Vânia Regina Nicoletti

Pink pepper essential oil was microencapsulated with chickpea protein (CP) and chickpea protein/pectin (CP-HMP) by spray drying. The reconstitution and storage properties of the powders were evaluated after drying. The impact of microencapsulation in the volatiles release, antioxidant and antimicrobial activity of oil was evaluated during 135 days of storage. CP resulted in more soluble powders (93.52%), CP/HMP resulted in denser powders (0.39 g/mL) while wall material did not influence the wettability. Free pink pepper essential oil (FEO) showed a slight loss of the predominant terpenes (α-pinene, β-pinene, β-mircene, δ-3-carene and D-limonene) after encapsulation. In general, all samples showed an increase in the volatiles release during storage. The evaluation of mass loss showed that FEO had a high release of volatiles, followed by CP and CP-HMP. The antioxidant activity of the FEO decreased (10.8 μg Trolox/mg of oil) after 135 days of storage, whereas the antioxidant activity of CP (14.9) and CP-HMP (14) increased. Both microcapsules presented antimicrobial activity against Bacillus subtilis and Staphylococcus aureus during storage. CP microcapsules had a strong inhibitory effect against the strains tested, and this advantage was even more evident in long-term storage.

通过喷雾干燥法将粉红胡椒精油与鹰嘴豆蛋白(CP)和鹰嘴豆蛋白/pectin(CP-HMP)进行微囊化。对干燥后粉末的重组和储存特性进行了评估。在 135 天的储存过程中,对微胶囊技术对油的挥发性物质释放、抗氧化和抗菌活性的影响进行了评估。氯化石蜡产生了更多的可溶性粉末(93.52%),氯化石蜡/HMP 产生了更致密的粉末(0.39 克/毫升),而壁材对润湿性没有影响。游离粉红胡椒精油(FEO)在封装后显示出主要萜烯(α-蒎烯、β-蒎烯、β-槲皮烯、δ-3-蒈烯和 D-柠檬烯)的轻微损失。总的来说,所有样品在储存期间的挥发性物质释放量都有所增加。质量损失评估表明,FEO 的挥发性物质释放量较高,其次是 CP 和 CP-HMP。储存 135 天后,FEO 的抗氧化活性下降(10.8 μg Trolox/mg油),而 CP(14.9)和 CP-HMP (14)的抗氧化活性上升。两种微胶囊在储存期间都具有抗枯草杆菌和金黄色葡萄球菌的活性。氯化石蜡微胶囊对所测试的菌株有很强的抑制作用,这种优势在长期储存中更为明显。
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引用次数: 0
Development and validation of paper-based strip method for the detection of formalin in milk 开发和验证检测牛奶中福尔马林的纸带方法
IF 2.701 Pub Date : 2024-05-27 DOI: 10.1007/s13197-024-06003-2
Farin Sayyad, Kamal Gandhi, Rajan Sharma, T. M. Amrutha, Priyae Brath Gautam, C. G. Harshitha

Formalin has been reported to be added to milk to prolong the shelf life of milk. The present study was conducted to develop and validate liquid-phase and paper-based strip methods for the rapid detection of formalin in milk using four different procedures i.e., A, B, C and D. Among all the procedures, procedure A worked successfully both in the liquid-phase and paper-based assays. The developed strip-based method involved the dipping of the strips in milk followed by visualization of change in colour. Formalin adulterated milk gave yellow colour to the strip, while for normal milk, it remained colourless. Limit of detection of developed strips was 0.05% with a response time of 7–8 min. Shelf life of the strips was a minimum of 6 months at both room (30–37 °C) and refrigeration (4 ℃) temperature. The strips can be used both at field as well as household levels for rapid detection of formalin presence in milk with greater sensitivity and hence can contribute in supplying authentic milk to the consumers.

据报道,在牛奶中添加福尔马林可延长牛奶的保质期。本研究采用四种不同的方法(即 A、B、C 和 D),开发并验证了液相和纸带法,用于快速检测牛奶中的福尔马林。所开发的纸条检测法是将纸条浸入牛奶中,然后观察颜色的变化。掺有福尔马林的牛奶会使试纸呈黄色,而正常牛奶则保持无色。检测条的检测限为 0.05%,反应时间为 7-8 分钟。在室温(30-37 °C)和冷藏(4 °C)条件下,检测条的保质期至少为 6 个月。该试剂条既可用于现场,也可用于家庭,以更高的灵敏度快速检测牛奶中是否含有福尔马林,从而有助于向消费者提供真正的牛奶。
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引用次数: 0
A low n-6/n-3 PUFA ratio and high level of dietary ɑ-linolenic acid improves sleep behavior in mice with insomnia 低 n-6/n-3 PUFA 比率和高水平的膳食 ɑ-亚麻酸可改善失眠小鼠的睡眠行为
IF 2.701 Pub Date : 2024-05-27 DOI: 10.1007/s13197-024-06004-1
Jie Zhu, Hua-nan Zhang, Jia-kai Wu, Fei-fei Li, Yu-qing Liu, Er-juan Ning, Li-qin Yu, Shen Liang

Camelina sativa oil (CSO) and Semen Ziziphi Spinosae oil (SZSO) are functional oils that have beneficial effects on brain health. This study evaluated the sedative and hypnotic effects of vegetable oils with various n − 6/n − 3 polyunsaturated fatty acids (PUFA) ratios and ɑ-linolenic acid (ALA) contents to mice. The n − 6/n − 3 PUFA ratios of CSO (CSO:SZSO = 1:0, 1.8 g/kg), SZSO (CSO:SZSO = 0:1, 1.8 g/kg), CSO-SZSO-L (CSO:SZSO = 1:1, 1.8 g/kg), and CSO-SZSO-H (CSO:SZSO = 1:1, 3.6 g/kg) were 0.51, 140, 1.69, and 1.69, respectively. The doses of ALA administered to mice with p-chlorophenylalanine-induced insomnia were approximately 0.64, 50 × 10–4, 0.32, and 0.64 g/kg, respectively. The mice were administered CSO, SZSO, and a low-dose combination of CSO and SZSO for seven days with no obvious hypnotic effects. However, the administration of a high-dose combination of CSO and SZSO significantly prolonged sleep duration in mice with induced insomnia and inhibited the serum levels of corticotropin-releasing hormone, adrenocorticotropic hormone, and cortisol. Interestingly, there were no significant effects on the structure and function of the hippocampal tissue. The results indicated that the anti-insomnia effects of these vegetable oils were positively correlated with a low n − 6/n − 3 PUFA ratio and the absolute amount of ALA.

荠菜油(CSO)和刺五加油(SZSO)是对大脑健康有益的功能性油脂。本研究评估了不同 n - 6/n - 3 多不饱和脂肪酸(PUFA)比例和ɑ-亚麻酸(ALA)含量的植物油对小鼠的镇静和催眠作用。CSO (CSO:SZSO = 1:0, 1.8 g/kg)、SZSO (CSO:SZSO = 0:1, 1.8 g/kg)、CSO-SZSO-L (CSO:SZSO = 1:1, 1.8 g/kg)和 CSO-SZSO-H (CSO:SZSO = 1:1, 3.6 g/kg)的 n - 6/n - 3 多不饱和脂肪酸比率分别为 0.51、140、1.69 和 1.69。对氯苯丙氨酸诱导失眠的小鼠服用的 ALA 剂量分别约为 0.64、50 × 10-4、0.32 和 0.64 克/千克。小鼠连续七天服用 CSO、SZSO 以及 CSO 和 SZSO 的低剂量复合制剂,均无明显催眠效果。然而,给小鼠服用大剂量的 CSO 和 SZSO 复方制剂可明显延长失眠小鼠的睡眠时间,并抑制血清中促肾上腺皮质激素释放激素、促肾上腺皮质激素和皮质醇的水平。有趣的是,对海马组织的结构和功能没有明显影响。结果表明,这些植物油的抗失眠作用与低 n - 6/n - 3 PUFA 比率和 ALA 的绝对量呈正相关。
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引用次数: 0
Effects of chrysanthemum extract on physicochemical, sensory, and in vitro digestion of set-type yogurt 菊花提取物对固定型酸奶理化、感官和体外消化的影响
IF 2.701 Pub Date : 2024-05-23 DOI: 10.1007/s13197-024-06000-5
Jun’an Zheng, Xin Li, Hongyue Wang, Di Yang, Jiajun Yin, Xiangrong Zhang

Chrysanthemum (Chrysanthemum morifolium Ramat) contains multiple bioactive substances and presents health benefits. In this study, a chrysanthemum extract (CE) was prepared by heat reflux extraction method and a functional yogurt was fabricated containing CE. According to the results of UPLC-MS/MS, 6 phenolic acids and 13 flavonoids were identified from CE compounds. The physicochemical and functional properties of yogurt during storage were investigated. In addition, rheology, microstructure and simulated digestion of yogurt on Day 1 were analyzed. The results demonstrated that the value of pH, total phenolic and flavonoid content, antioxidant capacity, the polyphenols stability in vitro gastric and intestinal digestion condition in the yogurt were enhanced. The syneresis, titratable acidity and the rate of protein hydrolysis in vitro gastric and intestinal digestion condition in the yogurt were reduced with CE addition. Moreover, the changes of protein network and sensory characteristics of yogurt were also occurred with addition of CE. These findings suggest that the integration of CE with yogurt is a promising way to improve the quality and storage ability of yogurt.

菊花(Chrysanthemum morifolium Ramat)含有多种生物活性物质,对健康有益。本研究采用热回流提取法制备了菊花提取物,并制作了含有菊花提取物的功能性酸奶。根据 UPLC-MS/MS 的结果,从菊花提取物中鉴定出 6 种酚酸和 13 种黄酮类化合物。研究了酸奶在储存过程中的物理化学和功能特性。此外,还分析了第 1 天酸奶的流变学、微观结构和模拟消化。结果表明,酸奶的 pH 值、总酚和类黄酮含量、抗氧化能力、多酚稳定性在体外胃肠消化条件下均有所提高。添加 CE 后,酸奶中的协同作用、可滴定酸度以及蛋白质在体外胃肠消化条件下的水解速度均有所降低。此外,添加 CE 后,酸奶的蛋白质网络和感官特征也发生了变化。这些研究结果表明,CE 与酸奶的结合是提高酸奶质量和贮藏能力的一种可行方法。
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引用次数: 0
Tropical fruits as a potential source for the recovery of bioactive compounds: Tamarindus indica L., Annona muricata, Psidium guajava and Mangifera indica 热带水果是回收生物活性化合物的潜在来源:罗望子、菠萝、番石榴和芒果
IF 2.701 Pub Date : 2024-05-22 DOI: 10.1007/s13197-024-05983-5
Mashell A. Toscano Oviedo, Luis A. García Zapateiro, Somaris E. Quintana

The objective of this review is to identify the bioactive compounds present in tropical fruits such as Tamarindus indica L., Annona muricata, Mangifera indica, and Psidium guajava and their biological activities. The identification of these compounds shows their potential as a food ingredient in the development of products, providing added value to them, because not only the pulp of the fruit is used, but also the shell and its other parts, such as the leaves, are used, being viable sources to obtain some compounds that benefit human health. Implementing fruits that have certain bioactive compounds such as carotenoids (β-carotene, α-carotene, lutein, zeaxanthin and β-cryptoxanthin), antioxidants (vitamins A and C), and phenolic compounds (ellagic acid, gallic acid, citric acid) in the production process in the food industry, allows them to become functional foodstuffs. The results obtained show the need to implement the operational processes that allow obtaining different compounds, which ensure their stability and precision, applying different extraction methods such as maceration, Soxhlet, supercritical fluids, and ultrasound.

本综述旨在确定热带水果中的生物活性化合物,如罗望子、莲雾、芒果和番石榴,以及它们的生物活性。对这些化合物的鉴定表明,它们具有作为食品配料开发产品的潜力,为产品提供了附加值,因为不仅使用了水果的果肉,还使用了果壳及其他部分,如叶子,这些都是获得有益于人类健康的某些化合物的可行来源。在食品工业的生产过程中,使用具有某些生物活性化合物的水果,如类胡萝卜素(β-胡萝卜素、α-胡萝卜素、叶黄素、玉米黄质和β-隐黄质)、抗氧化剂(维生素 A 和 C)和酚类化合物(鞣花酸、没食子酸、柠檬酸),可使它们成为功能性食品。研究结果表明,有必要采用不同的萃取方法,如浸渍法、索氏提取法、超临界流体提取法和超声波提取法,以获得不同的化合物,确保其稳定性和精确性。
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引用次数: 0
Automated liquid–liquid deep eutectic solvents based microextraction procedure for determination of acrylamide in foodstuffs by high-performance liquid chromatography with ultraviolet detection 利用高效液相色谱-紫外检测法测定食品中丙烯酰胺含量的基于液-液深共晶溶剂的自动微萃取程序
IF 2.701 Pub Date : 2024-05-22 DOI: 10.1007/s13197-024-05999-x
Andrey Shishov, Ulyana Markova, Davydova Ekaterina, Andrey Bulatov

For the rapid and efficient determination of acrylamide in food products by HPLC-UV, an environmentally friendly analytical approach has been developed, including liquid-phase extraction and subsequent liquid–liquid microextraction using a deep eutectic solvent. To automate the procedure, the flow-through “lab in a syringe” method was used. Acrylamide is considered a potential endocrine disrupting chemical and its main source is fried foods, which are widely consumed by both children and adults. To extract and concentrate acrylamide, hydrophobic deep-eutectic solvents based on various carboxylic acids and natural terpenoids were studied for the first time. It was found that benzoic acid, as a precursor of the extraction solvent, promotes the transfer of hydrophilic acrylamide from the aqueous phase of the sample due to the interaction of carboxyl and amide groups. The procedure has been validated and used effectively to estimate acrylamide content in beetroot and corn chips. Under optimal conditions, the detection limit was 0.01 mg/kg. Unlike existing methods, the proposed method is fully automated, does not require hazardous organic solvents and additional derivatization stages, and at the same time allows the determination of acrylamide at a level below established standards.

为了利用高效液相色谱-紫外法快速有效地测定食品中的丙烯酰胺含量,我们开发了一种环境友好型分析方法,包括液相萃取和随后使用深共晶溶剂的液液微萃取。为了使这一过程自动化,采用了 "注射器中的实验室 "流动法。丙烯酰胺被认为是一种潜在的干扰内分泌的化学物质,其主要来源是儿童和成人广泛食用的油炸食品。为了提取和浓缩丙烯酰胺,首次研究了基于各种羧酸和天然萜类化合物的疏水性深共晶溶剂。研究发现,苯甲酸作为萃取溶剂的前体,由于羧基和酰胺基的相互作用,可促进亲水性丙烯酰胺从样品水相中转移。该方法已通过验证,并可有效用于估算甜菜根和玉米片中的丙烯酰胺含量。在最佳条件下,检测限为 0.01 毫克/千克。与现有方法不同的是,所提出的方法是全自动的,不需要使用有害的有机溶剂和额外的衍生阶段,同时还能测定低于既定标准的丙烯酰胺含量。
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引用次数: 0
Inhibition of hepatic stellate cell activation by nutraceuticals: an emphasis on mechanisms of action 营养保健品抑制肝星状细胞活化:强调作用机制
IF 2.701 Pub Date : 2024-05-18 DOI: 10.1007/s13197-024-06002-3
Vasudevan Sekar, Venkateish VP, Vani Vijay, Annapoorna BR, Nivya Vijayan, Madan Kumar Perumal

Liver diseases emerge as a serious threat to humans worldwide due to increasing morbidity and mortality. Liver disease related deaths accounts for one third of all disease related death globally. A simple fatty liver if unattended advances further to liver fibrosis, cirrhosis and hepatocellular carcinoma. During liver fibrogenesis, hepatic stellate cells gets activated into myofibroblast like cells and exhibit proliferative and fibrogenic features. Targeting these activated hepatic stellate cells offer promising therapeutic approach towards liver fibrosis management. To date there is no Food and Drug Administration approved treatments for liver fibrosis. However, a large number of clinical trials are being conducted employing monoclonal antibodies, drugs, dietary supplements and herbal medicines. A vast number of research findings demonstrated nutraceuticals to be effective against experimental liver fibrosis both in vitro and in vivo. Nutraceuticals typically regulate key signaling pathways in activated hepatic stellate cells and exhibit anti-fibrotic effect. In this review, the mechanistic action of nutraceuticals targeting activated hepatic stellate cells were summarized to establish them as a possible therapeutic candidate for liver fibrosis.

由于发病率和死亡率不断上升,肝脏疾病已成为全球人类面临的严重威胁。全球与肝病相关的死亡人数占所有疾病相关死亡人数的三分之一。单纯性脂肪肝如果不加以重视,会进一步发展为肝纤维化、肝硬化和肝细胞癌。在肝纤维化过程中,肝星状细胞会被活化成类似肌成纤维细胞的细胞,并表现出增殖和纤维化特征。针对这些活化的肝星状细胞提供了治疗肝纤维化的有效方法。迄今为止,尚无食品和药物管理局批准的肝纤维化治疗方法。不过,大量临床试验正在采用单克隆抗体、药物、膳食补充剂和草药。大量研究结果表明,营养保健品在体外和体内都能有效防治实验性肝纤维化。营养保健品通常能调节活化的肝星状细胞中的关键信号通路,并表现出抗纤维化作用。本综述总结了以活化的肝星状细胞为靶点的营养保健品的作用机理,将其确立为治疗肝纤维化的可能候选疗法。
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引用次数: 0
Recovery of carotenoids as bioactive compounds from peach pomace by an eco-friendly ultrasound-assisted enzymatic extraction 利用环保型超声辅助酶提取法从桃渣中回收类胡萝卜素生物活性化合物
IF 2.701 Pub Date : 2024-05-18 DOI: 10.1007/s13197-024-06001-4
Kübra Nur Han, Hilal Meral, Aslıhan Demirdöven

The industrial processing of fruits generates by-products. These by-products serve as a source of valuable bioactive compounds. In this study, carotenoid was extracted from peach pomace (PP) by using the ultrasound-assisted enzymatic extraction (UAEE), an eco-friendly method. The process conditions ensuring the highest carotenoid content and b* color value for UAEE were detected by response surface methodology (RSM). To demonstrate the effectiveness of the ultrasonic process, enzymatic extraction was carried out at the optimum point. Physicochemical (pH, titratable acidity, total soluble solids), color (L*, a*, b*, chroma value (ΔC) and color difference (ΔE), total phenolic compound (TPC) and antioxidant activity analyses (ABTS and FRAP) were carried. When the analysis results evaluated, the highest b* color parameter, TPC (761.10 mg gallic acid/L), ABTS (1933.33 mg Trolox/L) and FRAP (52.66 µmol Trolox/L) results of the extracts was observed with UAEE method. The study shows that ultrasound based upon the cavitation event was increased efficiency of enzymatic reaction with higher extraction yield and this provided in higher amounts of carotenoid and bioactive compounds. In other respects, when obtained carotenoid extracts are used in food formulations compatible with their acidic structure, they will contribute to protection of the product and minimizing color losses.

水果的工业加工会产生副产品。这些副产品是宝贵的生物活性化合物的来源。本研究采用超声辅助酶解萃取法(UAEE)从桃渣(PP)中提取类胡萝卜素。采用响应面法(RSM)检测了确保 UAEE 中类胡萝卜素含量和 b* 色值最高的工艺条件。为了证明超声波工艺的有效性,在最佳点进行了酶提取。进行了理化(pH 值、可滴定酸度、总可溶性固形物)、颜色(L*、a*、b*、色度值 (ΔC) 和色差 (ΔE))、总酚化合物 (TPC) 和抗氧化活性分析(ABTS 和 FRAP)。分析结果表明,UAEE 法提取物的 b* 颜色参数、TPC(761.10 毫克没食子酸/升)、ABTS(1933.33 毫克三环氧化酶/升)和 FRAP(52.66 微摩尔三环氧化酶/升)结果最高。研究表明,基于空化事件的超声波提高了酶反应的效率,提高了提取率,从而获得了更多的类胡萝卜素和生物活性化合物。从其他方面来看,如果将获得的类胡萝卜素提取物用于与其酸性结构相适应的食品配方中,它们将有助于保护产品并最大限度地减少颜色损失。
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引用次数: 0
Discriminating three lab scale dark chocolate bars from fine Cameroon cocoa hybrids using sensorial evaluation and organic acid content 利用感官评估和有机酸含量鉴别喀麦隆优质可可杂交品种的三种实验室规模黑巧克力棒
IF 2.701 Pub Date : 2024-05-15 DOI: 10.1007/s13197-024-05998-y
Simon Perrez Akoa, Renaud Boulanger, Jude Manga Ndjaga, Pierre Effa Onomo, Marc Lebrun, Eliane Flore Eyenga, Gilles Morel, Raymond Ndip Nkongho, Pierre François Djocgoue

Previous studies have shown a correlation between chocolate sensory profile and certain (bio)chemical components. The aim of this study was to examine the sensorial profile and organic acid content of three lab scale chocolate brands produced from different cocoa genotypes. The sensorial evaluation was examined by a team of 12 panelists and evaluation of aroma volatiles was done by means of HS-SPME-GC-MS. On the other hand, organic acids were assessed using a high-performance ion chromatography coupled with an electrochemical detector (HPIC-ED). Results showed a variability in sensorial profile: SCA12×ICS40 chocolate (vanilla/sweet, spicy, and floral), ICS40 × SCA12 chocolate (fruity, bitter, and dry) and SNK16 × T60/887 chocolate (chocolate, honey-like, woody, sweet). Moreover, some aroma volatiles like (2-methyl, 3-methyl, iso) butanal (ICS), terpenes (SCA), and ketones (acetophenone and 2-nonanone) (SNK) allowed to discriminate dark chocolate sample according to their raw cocoa genetic group. Besides, the organic acid content differed from one chocolate brand to another, and it was obtained a high content of oxalic acid and a low lactic acid content which are good indicators of chocolate quality. Results of the current study highly recommend knowing the variety of cocoa beans with high content of some volatiles and high oxalic and low acetic acid and lactic contents to produce high aromatic (special flavor) chocolates.

以往的研究表明,巧克力的感官特征与某些(生物)化学成分之间存在关联。本研究的目的是检测由不同可可基因型生产的三种实验室规模巧克力品牌的感官特征和有机酸含量。感官评价由一个由 12 名专家组成的小组进行,香气挥发物的评价采用 HS-SPME-GC-MS 方法进行。另一方面,使用高效离子色谱法和电化学检测器(HPIC-ED)对有机酸进行了评估。结果显示,感官特征存在差异:SCA12×ICS40 巧克力(香草/甜味、辛辣味和花香)、ICS40 × SCA12 巧克力(果味、苦味和干味)和 SNK16 × T60/887 巧克力(巧克力、蜂蜜味、木香、甜味)。此外,一些香气挥发物,如(2-甲基、3-甲基、异)丁醛(ICS)、萜烯类(SCA)和酮类(苯乙酮和 2-壬酮)(SNK),可根据其原料可可基因组来区分黑巧克力样品。此外,不同品牌巧克力的有机酸含量也不同,草酸含量高,乳酸含量低,这些都是衡量巧克力质量的良好指标。目前的研究结果强烈建议了解一些挥发性物质含量高、草酸含量高、醋酸和乳酸含量低的可可豆品种,以生产高芳香(特殊风味)巧克力。
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引用次数: 0
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Journal of Food Science and Technology
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