The present study aimed to evaluate the color intensity, total phenolic content (TPC), total flavonoid content (TFC) and antioxidant activity of fresh honey samples. For this study, honey samples were collected from five regions and divided into three sites based on different floral and geographical origins: forest, rural, and urban sites located in North Maharashtra, India. The findings showed that honey samples from the selected sites were significantly different (p < 0.05). The honey samples tested had extra light amber to dark amber color variation with color intensity values of 450 to 907 mAU. The mean value of the TPC of the honey samples ranged from 179.34 to 461.41 mg GAE/kg, while the TFC ranged from 19.29 to 56.52 mg catechin/kg of honey. The ascorbic acid equivalent antioxidant content (AEAC) were ranged from 112.57 to 136.78 mg AEAC/kg of honey.
The findings indicated that honey from forest sites contains the highest phenolic, flavonoid and antioxidant contents with maximum color intensity compared to honey from rural and urban sites. The honey samples showed a strong positive correlation between color intensity, TPC, TFC, and antioxidant activity. As per PCA, the first component explained 73.4% of the variance, which was mostly dominated by TPC, TFC, and AEAC, whereas the second component, with a variance of 23.33%, was dominated by color intensity and color Pfund. Overall, North Maharashtra honey has a high level of antioxidants, suggesting that it could be a useful natural antioxidant source.