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Color intensity and antioxidant properties of honey produced from North Maharashtra, India 印度马哈拉施特拉邦北部蜂蜜的颜色强度和抗氧化特性
IF 2.701 Pub Date : 2024-12-21 DOI: 10.1007/s13197-024-06170-2
Arun Baburao Sawarkar

The present study aimed to evaluate the color intensity, total phenolic content (TPC), total flavonoid content (TFC) and antioxidant activity of fresh honey samples. For this study, honey samples were collected from five regions and divided into three sites based on different floral and geographical origins: forest, rural, and urban sites located in North Maharashtra, India. The findings showed that honey samples from the selected sites were significantly different (p < 0.05). The honey samples tested had extra light amber to dark amber color variation with color intensity values of 450 to 907 mAU. The mean value of the TPC of the honey samples ranged from 179.34 to 461.41 mg GAE/kg, while the TFC ranged from 19.29 to 56.52 mg catechin/kg of honey. The ascorbic acid equivalent antioxidant content (AEAC) were ranged from 112.57 to 136.78 mg AEAC/kg of honey.

The findings indicated that honey from forest sites contains the highest phenolic, flavonoid and antioxidant contents with maximum color intensity compared to honey from rural and urban sites. The honey samples showed a strong positive correlation between color intensity, TPC, TFC, and antioxidant activity. As per PCA, the first component explained 73.4% of the variance, which was mostly dominated by TPC, TFC, and AEAC, whereas the second component, with a variance of 23.33%, was dominated by color intensity and color Pfund. Overall, North Maharashtra honey has a high level of antioxidants, suggesting that it could be a useful natural antioxidant source.

本研究旨在评价新鲜蜂蜜样品的颜色强度、总酚含量(TPC)、总黄酮含量(TFC)和抗氧化活性。在这项研究中,蜂蜜样本从五个地区收集,并根据不同的花卉和地理来源分为三个地点:位于印度北部马哈拉施特拉邦的森林、农村和城市地点。结果表明,所选地点的蜂蜜样品有显著差异(p < 0.05)。所测蜂蜜样品有额外的浅琥珀色到深琥珀色的变化,颜色强度值为450至907 mAU。蜂蜜样品的TPC平均值为179.34 ~ 461.41 mg GAE/kg, TFC平均值为19.29 ~ 56.52 mg儿茶素/kg。蜂蜜抗坏血酸当量抗氧化剂含量(AEAC)为112.57 ~ 136.78 mg /kg。结果表明,与农村和城市蜂蜜相比,森林蜂蜜的酚类、类黄酮和抗氧化剂含量最高,颜色强度最大。蜂蜜样品的颜色强度、TPC、TFC与抗氧化活性呈正相关。主成分分析表明,第一个分量解释了73.4%的方差,其中以TPC、TFC和AEAC为主;第二个分量解释了23.33%的方差,以颜色强度和颜色Pfund为主。总的来说,北马哈拉施特拉蜂蜜含有高水平的抗氧化剂,这表明它可能是一种有用的天然抗氧化剂来源。
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引用次数: 0
Developing novel synbiotic low-fat yogurt using sour cherry pomace pectin-derived oligosaccharides: Lactobacillus acidophilus survival, quality and antioxidant properties 利用酸樱桃果渣果胶衍生低聚糖开发新型合成低脂酸奶:嗜酸乳杆菌的存活、品质和抗氧化性能。
IF 2.701 Pub Date : 2024-12-20 DOI: 10.1007/s13197-024-06162-2
Karim Parastouei, Faramarz Khodaiyan, Seyed Saeid Hosseini

A new high potential synbiotic yogurt with remarkable functionality was produced.

Sour cherry pectin hydrolysates with high antioxidants were applied as prebiotics.

Physicochemical, bioactivity and sensory properties of the yogurts were determined.

Hydrolysates improved significantly the antioxidant and sensory properties.

Hydrolysates increased the viability of probiotic bacteria.

采用微波辅助提取酸樱桃果渣中的果胶,并用果胶酶水解得到的上清液。然后,以不同浓度(0.1、0.4、0.8和1.2% w/v)的果胶寡糖(POS)为益生元,嗜酸乳杆菌为益生菌,生产低脂合成酸奶。结果表明,当添加量达到0.4% w/v时,其酸度、黏度和益生菌活力显著增加,pH值和协同作用显著降低。此外,这些富含抗氧化剂的水解物的加入增加了生产的酸奶的抗自由基活性。此外,低浓度的POS改善了感官特性,而这些特性在较高浓度的水解物存在下会降低。总的来说,可以这样说,酸樱桃果胶水解物在低浓度下可以用来开发一种很好的具有高感官特性的合成酸奶。补充资料:在线版本提供补充资料,网址为10.1007/s13197-024-06162-2。
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引用次数: 0
Assessment of fermented milk tablets containing Lacticaseibacillus paracasei SD1 and Lacticaseibacillus rhamnosus SD11 in postbiotic and live probiotic forms: an in vitro study 含有副干酪乳杆菌SD1和鼠李糖乳杆菌SD11的发酵乳片在生后和活益生菌形式中的评估:一项体外研究
IF 2.701 Pub Date : 2024-12-19 DOI: 10.1007/s13197-024-06172-0
Rawee Teanpaisan, Suchera Thananimit, Nuntiya Pahumunto

Fermented milk is commonly used to preserve live probiotics, but its limited shelf life is a challenge. Tablet forms offer an innovative solution, and recent researches indicate that postbiotics can provide similar benefits to live probiotics. This study aimed to optimize fermented milk preparation using Lacticaseibacillus paracasei SD1 and Lacticaseibacillus rhamnosus SD11 and to assess their effectiveness when incorporated into fermented milk tablets. The study investigated the effects of temperatures (30 °C and 45 °C) for fermented milk preparation using single and combined probiotic strains and then examined anti-bacterial activity, suppression of pro-inflammatory cytokines, growth inhibition of Caco-2 cells, and butyrate concentration. Additionally, fermented milk tablets containing either postbiotics or live probiotics were prepared and evaluated for efficacy and stability. The results demonstrated that fermented milk at 45 °C using a 1:1 combination of L. paracasei SD1 and L. rhamnosus SD11 exhibited probiotic potential in all parameters. As a result, this formulation was selected to prepare fermented milk tablets. The tablets, whether containing postbiotics or live probiotics, effectively inhibited pathogen growth, reduced pro-inflammatory cytokine levels, suppressed Caco-2 cell proliferation, and promoted butyrate production. The beneficial efficacy remained stable for 6 months of storage, and fermented milk tablets maintained probiotic levels above 8 log cells g−1 after 6 months at 4 °C. However, at 25 °C, live probiotic tablets lost viability after 2 months, while postbiotic tablets retained heat-killed cells at 4 log cells g−1 for 6 months. Therefore, fermented milk tablets may be an alternative way to preserve probiotics and their biological benefits for longer.

发酵乳通常用于保存活的益生菌,但其有限的保质期是一个挑战。片剂形式提供了一种创新的解决方案,最近的研究表明,后益生菌可以提供与活益生菌相似的益处。本研究旨在优化副干酪乳杆菌SD1和鼠李糖乳杆菌SD11在发酵乳制剂中的应用,并评价其在发酵乳片剂中的应用效果。本研究考察了温度(30°C和45°C)对单一和组合益生菌菌株发酵乳制剂的影响,然后检测了抗菌活性、对促炎细胞因子的抑制、对Caco-2细胞的生长抑制和丁酸盐浓度。此外,还制备了含有益生菌或活益生菌的发酵乳片,并对其功效和稳定性进行了评估。结果表明,在45℃条件下,以1:1比例添加副干酪乳杆菌SD1和鼠李糖乳杆菌SD11的发酵乳在所有条件下均表现出益生菌潜力。因此,选择该配方制备发酵乳片。该片剂无论含有生后制剂还是活菌制剂,均能有效抑制病原菌生长,降低促炎细胞因子水平,抑制Caco-2细胞增殖,促进丁酸盐生成。发酵乳片在4℃条件下保存6个月后,益生菌水平保持在8 log cells g−1以上。然而,在25°C下,活的益生菌片在2个月后失去活力,而后生物片在4 log细胞g−1的温度下保留了6个月的热杀死细胞。因此,发酵乳片可能是长期保存益生菌及其生物效益的另一种方法。
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引用次数: 0
Optimization and characterization of nutrient-fortified milk beverages using conjoint analysis and response surface methodology 用联合分析和响应面法优化和表征营养强化乳饮料
IF 2.701 Pub Date : 2024-12-19 DOI: 10.1007/s13197-024-06167-x
Ramachandran Ramkumar, Ayyavoo Preamnath Manoharan, Shyam Arjunan, Pugazhenthi Thirumalai Rengasamy, Sivakumar Pitchaimuthu, Jerish Joyner Janahar, Senthilkumar Ganesamoorthy

In this study, we developed fortified milk beverages (FMB) with varying ingredient levels and optimized both ingredient levels and retort processing conditions using conjoint analysis and response surface methodology (RSM). The optimal retort processing conditions were determined through a Box–Behnken design in RSM, identifying 100 °C for 10 min as the preferred combination. This optimized condition yielded FMB with desirable properties, including pH values from 6.57 to 6.30, titratable acidity from 0.140 to 0.170%, total soluble solids from 15.55 to 17.07° Brix, peroxide values from 0.64 to 2.07 meq O2/Kg, and viscosity from 1.68 to 1.46 cp over 90 days. Significant differences (P < 0.05) were observed between control and fortified samples in terms of moisture, fat, crude fiber, crude protein, and caloric value. During storage, concentrations of docosahexaenoic acid and vitamin A in the FMB decreased to 6.22 and 3.38 mg/200 mL, respectively. Sensory evaluations were conducted on both control and fortified samples throughout their shelf life.

在这项研究中,我们开发了不同成分水平的强化乳饮料(FMB),并利用联合分析和响应面法(RSM)优化了成分水平和蒸煮工艺条件。在RSM中通过Box-Behnken设计确定了最佳的蒸馏塔工艺条件,确定了100°C 10 min为首选组合。优化后的FMB在90天内的pH值在6.57 ~ 6.30之间,可滴定酸度在0.140 ~ 0.170%之间,总可溶性固溶体在15.55 ~ 17.07°白度之间,过氧化值在0.64 ~ 2.07 meq O2/Kg之间,粘度在1.68 ~ 1.46 cp之间。对照组和强化样品在水分、脂肪、粗纤维、粗蛋白质和热值方面存在显著差异(P < 0.05)。贮藏期间,FMB中二十二碳六烯酸和维生素A的浓度分别降至6.22和3.38 mg/200 mL。在整个保质期内对对照和强化样品进行了感官评价。
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引用次数: 0
Preparation of petha from pointed gourd (Trichosanthes dioica) mediated by osmotic dehydration and its characterization 渗透脱水法制备尖葫芦petha及其表征
IF 2.701 Pub Date : 2024-12-18 DOI: 10.1007/s13197-024-06159-x
Kaustubh Singh, Muskan Kumari, Anil Kumar Chauhan, Satya Vir Singh, Milica Pojić, Shraddha Prakash

The subject of investigation in this research was the preparation of pointed gourd (Trichosanthes dioica) petha using osmotic dehydration. The primary objective of this study was to explore the use of osmotic dehydration as a method for the preparation of petha from pointed gourd. The process entails using sugar as an osmotic agent for the peeled and deseeded pointed gourd, subjecting it to different temperature conditions (60˚C and 70˚C) in tray dryer. Nevertheless, the effects of the treatments were compared based on nutritional value, total phenolics and as well as textural and sensory properties. The sample (B1) with the highest sensory quality was obtained at pointed gourd treated with sugar solution of 30ºBx at 60˚C for 9 h. Further, the product was characterized using Fourier transform infrared spectroscopy and scanning electron microscopy. The results showed that the osmotic dehydration can be an effective method for enhancing the storage life of product in terms of both sensory as well as nutritional quality.

本研究以渗透脱水法制备尖葫芦为研究对象。本研究的主要目的是探索利用渗透脱水法制备尖葫芦佩塔的方法。该工艺需要使用糖作为渗透剂,将去皮和去籽的尖葫芦置于不同的温度条件下(60˚C和70˚C)在托盘干燥器中。然而,根据营养价值、总酚类物质以及质地和感官特性,比较了处理的效果。用60˚C、30ºBx的糖溶液处理尖葫芦9 h,得到感官品质最高的样品(B1)。并用傅里叶变换红外光谱和扫描电镜对产物进行了表征。结果表明,渗透脱水是提高产品感官品质和营养品质的有效方法。
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引用次数: 0
Studies on in vitro antioxidant potentials and characterization of κ-carrageenan based biodegradable film from red algae, Hypnea valentiae (Turner) Montagne, 1841 红藻(Hypnea valentiae (Turner) Montagne, 1841)中κ-卡拉胶基可生物降解膜的体外抗氧化性能及表征
IF 2.701 Pub Date : 2024-12-15 DOI: 10.1007/s13197-024-06147-1
Raja Priya Rajamani, Samanta Sekhar Khora

Seaweeds are a rich source of various bioactive compounds and metabolites. In this study, sulfated polysaccharide, κ-carrageenan has been isolated from red algae, Hypnea valentiae, and developed biodegradable film with the addition of plasticizers, sorbitol and polyethylene glycol (PEG 4000) at varying concentrations of carrageenan (1%, 5% and 7% Carr). The biodegradable film exhibited excellent mechanical properties with increasing carrageenan concentration. The 5% Carr film showed improved water barrier properties, high water contact angle (hydrophobicity), high tensile strength (11.38 MPa) and elongation at break (23.27%) which imparts rigidity and flexibility to the film. The results of FTIR and SEM images revealed that the plasticizers and polysaccharides have been blended properly. Since seaweeds are natural antioxidants the κ-carrageenan based biodegradable films displayed strong radical scavenging activity. The 7% Carr film exhibited 86% of radical scavenging activity in ABTS assay and 5% Carr showed 80% of scavenging activity. The biodegradable films displayed efficient antibacterial activity against gram-negative bacteria, E. coli and gram-positive bacteria, B. subtilis. The developed films exhibited excellent biodegradability, the films samples degraded completely within 84 days of soil burial. The κ-carrageenan based biodegradable film (5% Carr) from red algae, H. valentiae better serves as a versatile food packaging material.

Graphical abstract

海藻是各种生物活性化合物和代谢物的丰富来源。本研究从红藻Hypnea valentiae中分离出硫酸酸化多糖κ-卡拉胶,并在不同卡拉胶浓度(1%、5%和7% Carr)下,通过添加增塑剂、山梨醇和聚乙二醇(PEG 4000),制备出可生物降解的膜。随着卡拉胶浓度的增加,生物可降解膜表现出优异的力学性能。5% Carr膜具有较好的水阻隔性能、较高的水接触角(疏水性)、较高的抗拉强度(11.38 MPa)和断裂伸长率(23.27%),具有较好的刚性和柔韧性。红外光谱(FTIR)和扫描电镜(SEM)分析结果表明,增塑剂与多糖的混合效果良好。由于海藻是天然抗氧化剂,κ-卡拉胶基生物可降解膜具有很强的自由基清除活性。在ABTS实验中,7% Carr膜的自由基清除活性为86%,5% Carr膜的自由基清除活性为80%。该生物降解膜对革兰氏阴性菌大肠杆菌和革兰氏阳性菌枯草芽孢杆菌具有良好的抑菌活性。冲洗后的膜具有良好的生物降解性,样品在土壤掩埋84天内完全降解。以红藻(H. valentiae)为原料制备的κ-卡拉胶基可生物降解薄膜(5% Carr)是一种多功能的食品包装材料。图形抽象
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引用次数: 0
The impact of plant proteases and sous vide technology on quality characteristics of meat 植物蛋白酶和索氏蒸煮技术对肉类质量特性的影响
IF 2.701 Pub Date : 2024-12-14 DOI: 10.1007/s13197-024-06151-5
Nuran Erdem, Süleyman Gökmen

This study aims to assess the impact of protease-rich fruit (kiwi), a rhizome (ginger), and sous vide technology (SVT) on the technological, textural, microstructural, microbiological, and sensory properties of different types of meat, including beef, goose, and rabbit. It was observed that all types of meat treated with kiwi and ginger showed a significant decrease (p < 0.05) in pH and a significant increase (p < 0.05) in water holding capacity and L* parameters compared to those treated with distilled water. The addition of ginger, which contains the enzyme zingibain, was found to enhance the a* parameter across all types of meat. The Texture Profile Analysis (hardness, springiness, gumminess, chewiness) and Extended Craft Knife parameters (firmness and work of shear) in the meat samples significantly decreased (p < 0.05) following the combined treatment of marination and SVT. The study identified zingibain, an enzyme found in ginger, as the most effective for tenderizing beef (p < 0.05). In contrast, actinidin, the enzyme present in kiwi, was found to be the most effective for tenderizing goose and rabbit meats. The microstructural analysis showed significantly larger spaces between muscle fibers in the treated meats compared to the control samples. The combination of plant protease and SVT significantly reduced (p < 0.05) the microorganism count in the meats and received higher texture scores from the panelists (p < 0.05). Furthermore, the purees did not cause any sensory defects.

Graphical abstract

本研究旨在评估富含蛋白酶的水果(猕猴桃)、根茎(生姜)和真空烹调技术(SVT)对不同类型肉类(包括牛肉、鹅肉和兔肉)的工艺、质地、微观结构、微生物和感官特性的影响。结果表明,与蒸馏水处理的肉相比,猕猴桃和生姜处理的肉的pH值显著降低(p < 0.05),持水量和L*参数显著增加(p < 0.05)。研究发现,添加含有青豆酶的生姜可以提高所有肉类的a*参数。腌制和SVT联合处理后,肉样的质地分析(硬度、弹性、胶性、咀嚼性)和扩展工艺刀参数(硬度和剪切功)显著降低(p < 0.05)。该研究发现,生姜中的一种酶——青豆素,对牛肉的嫩化最有效(p < 0.05)。相反,猕猴桃中含有的actitinidin酶被发现对鹅肉和兔肉的嫩化最有效。微观结构分析显示,与对照样品相比,处理过的肉类中肌肉纤维之间的空间明显更大。植物蛋白酶和SVT的组合显著降低了肉品中的微生物数量(p < 0.05),并获得了小组成员更高的质地评分(p < 0.05)。此外,果泥没有引起任何感官缺陷。图形抽象
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引用次数: 0
Exploring multi-ethnicity consumer acceptance of migratory locust (Locusta migratoria)-based food products: a survey of Malaysian consumers 探索多种族消费者对迁徙蝗(Locusta migratoria)为基础的食品的接受程度:对马来西亚消费者的调查
IF 2.701 Pub Date : 2024-12-13 DOI: 10.1007/s13197-024-06154-2
Vigneswary Gunasangar, Nur Afiqah Suadi, Mohammad Alrosan, Muhammad H. Alu’datt, Azhar Mat Easa, Thuan-Chew Tan

A cross-sectional survey research design was chosen to explore the degree of acceptance of Malaysian consumers from various demographic backgrounds towards migratory locust-based food products by using the Food Neophobia Scale (FNS), Insect Phobia Scale (IPS), and Willingness to Consume (WTC) migratory locust-based food products scale. This study chose migrant locusts (Locusta migratoria) due to their high protein content and potential to obtain Halal certification and sustainable production. In this study, 326 questionnaires were distributed online using non-probability sampling methods among Malaysian consumers. The results showed that average mean values for total FNS, IPS, and WTC scores were 35.73 out of 70, 31.05 out of 42, and 4.27 out of 7, respectively. In addition, the correlations between FNS and IPS scores towards WTC scores showed a negative correlation, with the IPS-WTC (− 0.399) correlation being stronger than FNS-WTC (− 0.216). Regarding demographic background, statistically significant variables for total FNS and IPS scores were limited to age group and gender. Overall, it can be concluded that most respondents hold a neutral stance towards introducing migratory locust-based food products in Malaysia, with entomophobia being the most probable reason for consumers’ rejection of migratory locust-based food products.

采用横断面调查研究设计,通过使用食物恐惧症量表(FNS)、昆虫恐惧症量表(IPS)和消费意愿量表(WTC),探讨马来西亚不同人口背景的消费者对迁徙蝗虫食品的接受程度。本研究选择迁徙蝗虫(Locusta migratoria)是因为它们的蛋白质含量高,有可能获得清真认证和可持续生产。在本研究中,326份问卷采用非概率抽样方法在马来西亚消费者中进行在线分发。结果显示,FNS、IPS和WTC总分的平均平均值分别为35.73分(满分70分)、31.05分(满分42分)和4.27分(满分7分)。此外,FNS评分与IPS评分对WTC评分的相关性呈负相关,其中IPS-WTC(- 0.399)的相关性强于FNS-WTC(- 0.216)。关于人口统计学背景,FNS和IPS总分的统计显著变量仅限于年龄组和性别。总体而言,可以得出结论,大多数受访者对在马来西亚引进迁徙蝗虫食品持中立态度,昆虫恐惧症是消费者拒绝迁徙蝗虫食品的最可能原因。
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引用次数: 0
Application of neutral electrolyzed water enhanced with organic acids as an antibacterial activity for chicken breast preservation 有机酸增强中性电解水在鸡胸肉防腐中的应用
IF 2.701 Pub Date : 2024-12-13 DOI: 10.1007/s13197-024-06157-z
Seyyed Mohammad Ali Noori, Mohammad Hashemi, Maliheh Dousti Noori, Maryam Seyedtabib, Shiva Adibi, Sara Bahrainian, Reza Abedi-Firoozjah, Milad Tavassoli

The research aimed to assess the effectiveness of neutral electrolyzed water, alone or combined with acetic acid and lactic acid, in reducing Listeria monocytogenes and Salmonella enteritidis in chicken breast. The research involved evaluating quality parameters, physical and chemical properties, bacterial count, and sensory characteristics of chicken breast samples over 0, 1, 3, 6, and 9 days. The findings showed that neutral electrolyzed water had better antibacterial activity than distilled water. The inclusion of acetic and lactic acids amplified the inhibitory effect of neutral electrolyzed water, with the most substantial impact on the growth of the target bacteria observed when both acids were utilized together. The treatments had a more pronounced effect on the gram-negative bacteria S. enteritidis (2.85 ± 2.8 log CFU/g) compared to the gram-positive bacteria L. monocytogenes (2.9 ± 1.6 log CFU/g). The physicochemical quality and microbial analysis (e.g., TVB-N, pH, PV, PTC, TVC, and TBA) of chicken samples showed significant changes in samples containing L. monocytogenes compared to samples containing S. enteritidis bacteria. The study concluded that combining neutral electrolyzed water with organic acids (NEW + AA-2 + LA-2) is a promising approach to extend the shelf life of chicken samples due to competitive production costs, safe transportation, low corrosion capacity, and low toxicity.

本研究旨在评估中性电解水单独或与醋酸和乳酸联合使用对减少鸡胸肉中单核增生李斯特菌和肠炎沙门氏菌的效果。该研究包括在0、1、3、6和9天内评估鸡胸肉样品的质量参数、物理和化学特性、细菌计数和感官特性。结果表明,中性电解水的抑菌活性优于蒸馏水。醋酸和乳酸的加入放大了中性电解水的抑制作用,当两种酸一起使用时,对目标菌的生长影响最大。对革兰氏阴性肠炎链球菌(2.85±2.8 log CFU/g)的影响明显高于革兰氏阳性单核增生乳杆菌(2.9±1.6 log CFU/g)。鸡肉样品的理化质量和微生物分析(如TVB-N、pH、PV、PTC、TVC和TBA)显示,含单增乳杆菌的样品与含肠炎沙门氏菌的样品存在显著差异。该研究得出结论,中性电解水与有机酸(NEW + AA-2 + LA-2)结合是一种很有前途的延长鸡肉样品保质期的方法,因为它具有生产成本竞争力、运输安全、低腐蚀能力和低毒性。
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引用次数: 0
Leveraging insect-derived mulberry sericin for high-protein white bread: a case study on dough optimization, techno-functional properties and sensory acceptability 利用昆虫衍生的桑蚕丝胶制作高蛋白白面包:面团优化、技术功能特性和感官可接受性的案例研究
IF 2.701 Pub Date : 2024-12-13 DOI: 10.1007/s13197-024-06155-1
Salony Azam Sheikh, T. Tamilselvan, Janani Ramesh, Abhilasha Rangi, Y. C. Radhalakshmi, Pichan Prabhasankar, Sridevi Annapurna Singh

In response to the emerging demand for alternative protein sources in the food industry, this study explores the incorporation of insect-derived sericin from Bombyx mori in high-protein white bread. The sericin exhibited 91% purity and a unique amino acid profile, rich in serine (~ 29%), aspartic acid (~ 18%), glutamic acid (~ 9%), and threonine (~ 5%). The study investigates the impact of sericin on dough properties, bread structure, nutritional content, and sensory attributes. Optimal dough stability and sensory acceptance were achieved with 5% sericin, 0.010% α-amylase, and 2.5% glycerol monostearate (GMS). While sericin incorporation initially reduced loaf volume (from 378 cc for control to 220 cc with 15% sericin), the addition of α-amylase (0.005 − 0.015%) and GMS paste (2.5%) optimized the bread’s volume and structure, resulting in a 30% increase in protein content compared to the control. The optimized bread exhibited fewer but larger air pockets (162 cells, ~ 55 mm³), indicating improved dough aeration. The integration of mulberry sericin into white bread was successfully achieved through the combined effects of sericin, α-amylase, and GMS paste, ensuring enhanced protein content, improved texture, and sensory acceptability. Therefore, this study provides insights into the potential for utilizing sericin as a sustainable protein source in bread formulations.

Graphical Abstract

为了响应食品工业对替代蛋白质来源的新兴需求,本研究探索了在高蛋白白面包中加入家蚕昆虫来源的丝胶。丝氨酸纯度为91%,具有独特的氨基酸结构,富含丝氨酸(~ 29%)、天冬氨酸(~ 18%)、谷氨酸(~ 9%)和苏氨酸(~ 5%)。研究了丝胶对面团性质、面包结构、营养成分和感官特性的影响。在5%丝胶、0.010% α-淀粉酶和2.5%单硬脂酸甘油(GMS)的条件下,面团稳定性和感官接受度最佳。丝胶蛋白的掺入使面包的体积从对照的378 cc降低到添加15%丝胶蛋白时的220 cc,而α-淀粉酶(0.005 ~ 0.015%)和GMS浆料(2.5%)的掺入使面包的体积和结构得到优化,蛋白质含量比对照提高了30%。优化后的面包具有较少但较大的气穴(162个,~ 55 mm³),表明面团的通气性得到改善。通过丝胶蛋白、α-淀粉酶和GMS膏体的联合作用,成功地将桑蚕丝胶蛋白整合到白面包中,提高了白面包的蛋白质含量,改善了白面包的质地,提高了白面包的感官接受度。因此,这项研究为利用丝胶蛋白作为面包配方中可持续蛋白质来源的潜力提供了见解。图形抽象
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Journal of Food Science and Technology
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