Protein--protein interaction (PPI) is a major way for proteins to perform their biological functions. Due to the lack of the PPI experimental data, theoretical prediction seems important to understand protein functions. Up to now, there have been various computational methods as for how to predict PPI. This report, however, will present a novel approach to the prediction of PPI by analyzing protein secondary structures. In the model, a support vector machine (SVM) was trained through a positive data set and a negative data set, each of which contains 7,714 protein pairs involving 1,730 proteins. To select PPI pairs in the training negative data sets, a new parameter that describes protein interaction relative bias (PIRB) was introduced as a measure of PPI propensity. The prediction accuracy was 88.01% when the model was employed to predict PPI in Saccharomyces cerevisiae.
{"title":"Prediction of Protein-Protein Interactions in Saccharomyces cerevisiae Based on Protein Secondary Structure","authors":"L. Cai, Zhiyong Pei, Sheng Qin, Xiu-juan Zhao","doi":"10.1109/ICBEB.2012.302","DOIUrl":"https://doi.org/10.1109/ICBEB.2012.302","url":null,"abstract":"Protein--protein interaction (PPI) is a major way for proteins to perform their biological functions. Due to the lack of the PPI experimental data, theoretical prediction seems important to understand protein functions. Up to now, there have been various computational methods as for how to predict PPI. This report, however, will present a novel approach to the prediction of PPI by analyzing protein secondary structures. In the model, a support vector machine (SVM) was trained through a positive data set and a negative data set, each of which contains 7,714 protein pairs involving 1,730 proteins. To select PPI pairs in the training negative data sets, a new parameter that describes protein interaction relative bias (PIRB) was introduced as a measure of PPI propensity. The prediction accuracy was 88.01% when the model was employed to predict PPI in Saccharomyces cerevisiae.","PeriodicalId":6374,"journal":{"name":"2012 International Conference on Biomedical Engineering and Biotechnology","volume":"124 1","pages":"413-416"},"PeriodicalIF":0.0,"publicationDate":"2012-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77100158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: To investigate the feasibility of a four-dimensional computerized tomography (4D-CT) reconstruction method based on the similarity principle of spatial adjacent images and mutual information measure. Methods: The CT data of patient underwent free breathing were acquired in Cine mode by a GE Light Speed 16 CT scanner, the Cine during time was equal to the breathing period of the patient plus one second (about 4-6s) and 8 to 15 slices CT image were acquired by a row CT detector in every Cine scan. A software program was developed by using VC++ and VTK software tools to re-sort the CT data and reconstruct the 4D-CT. Then all of the CT data with same phase were sorted by the program into a same series based on the similarity principle of spatial adjacent images and mutual information measure among them, and 3D reconstruction of different phase CT data were completed by using the software. Results: All of the CT data were sort accurately into different series based on the similarity principle of spatial adjacent images and mutual information measures among them. Compared with the unsorted CT data, the motion artifacts in the 3D reconstruction of sorted CT data were reduced significantly, and all of the sorted CT series result in a 4D-CT that reflected the characteristic of the free breathing. Conclusion: Time-resolved 4D-CT reconstruction can be implemented with any general multi-slice CT scanners based on the similarity principle of spatial adjacent images and mutual information measure, the process of the 4D--CT data acquisition and reconstruction were not restricted to the hardware or software of the CT scanner and has the feasibility and extensive applicability.
{"title":"Four-Dimensional Computerized Tomography (4D-CT) Reconstruction without an External Breath Surrogate","authors":"Shu-xu Zhang, Yu Hui, Sheng-qu Lin, Hai-nan Zhang","doi":"10.1109/ICBEB.2012.229","DOIUrl":"https://doi.org/10.1109/ICBEB.2012.229","url":null,"abstract":"Objective: To investigate the feasibility of a four-dimensional computerized tomography (4D-CT) reconstruction method based on the similarity principle of spatial adjacent images and mutual information measure. Methods: The CT data of patient underwent free breathing were acquired in Cine mode by a GE Light Speed 16 CT scanner, the Cine during time was equal to the breathing period of the patient plus one second (about 4-6s) and 8 to 15 slices CT image were acquired by a row CT detector in every Cine scan. A software program was developed by using VC++ and VTK software tools to re-sort the CT data and reconstruct the 4D-CT. Then all of the CT data with same phase were sorted by the program into a same series based on the similarity principle of spatial adjacent images and mutual information measure among them, and 3D reconstruction of different phase CT data were completed by using the software. Results: All of the CT data were sort accurately into different series based on the similarity principle of spatial adjacent images and mutual information measures among them. Compared with the unsorted CT data, the motion artifacts in the 3D reconstruction of sorted CT data were reduced significantly, and all of the sorted CT series result in a 4D-CT that reflected the characteristic of the free breathing. Conclusion: Time-resolved 4D-CT reconstruction can be implemented with any general multi-slice CT scanners based on the similarity principle of spatial adjacent images and mutual information measure, the process of the 4D--CT data acquisition and reconstruction were not restricted to the hardware or software of the CT scanner and has the feasibility and extensive applicability.","PeriodicalId":6374,"journal":{"name":"2012 International Conference on Biomedical Engineering and Biotechnology","volume":"42 1","pages":"814-816"},"PeriodicalIF":0.0,"publicationDate":"2012-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77511402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Potential allelopathic or toxic effect of some plant species can be useful for controlling snail acting as intermediate host of schistosomes. We reported on the toxic activity of extract from Sapium sebiferum against snails transmitting Schistosoma japonicum. Aqueous extract, ethanol extract, acetic ether extract or benzene extract from leaves, branches, roots and seeds of Sapium sebiferum have toxic effect on snail host. Benzene fraction from fresh leaves and deciduous leaves can also kill snail at different mortality. Benzene fraction of fresh leaves decreased head protein content, liver protein content and glycogen of snails submerged at different concentrations and different days. We concluded that Sapium sebiferum is an effective molluscicidal plant species.
{"title":"Effect and Machanism of Sapium sebiferum Against Snail (Oncomelania hupensis) and Control of Schistosomiasis","authors":"Qixiang Sun, Y. Yang, Qian Zhang","doi":"10.1109/ICBEB.2012.165","DOIUrl":"https://doi.org/10.1109/ICBEB.2012.165","url":null,"abstract":"Potential allelopathic or toxic effect of some plant species can be useful for controlling snail acting as intermediate host of schistosomes. We reported on the toxic activity of extract from Sapium sebiferum against snails transmitting Schistosoma japonicum. Aqueous extract, ethanol extract, acetic ether extract or benzene extract from leaves, branches, roots and seeds of Sapium sebiferum have toxic effect on snail host. Benzene fraction from fresh leaves and deciduous leaves can also kill snail at different mortality. Benzene fraction of fresh leaves decreased head protein content, liver protein content and glycogen of snails submerged at different concentrations and different days. We concluded that Sapium sebiferum is an effective molluscicidal plant species.","PeriodicalId":6374,"journal":{"name":"2012 International Conference on Biomedical Engineering and Biotechnology","volume":"52 1","pages":"190-193"},"PeriodicalIF":0.0,"publicationDate":"2012-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77783377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xin Ma, Shubao Yang, W. Jin, Yunjiao Zhao, Hongxia Ma, W. Luan
Major royal jelly protein 1 (MRJP1, the most abundant protein of honeybee royal jelly) has been recognized as a valuable functional foods and therapeutic protein for human beings. There is great potential using transgenic technology to express recombinant MRJP1 in milk in order to improve the human health conditions. For expression of MRJP1 in milk, mammary gland-specific expression vector (pBC1-MRJP1-NEOr) carrying the expression cassette composed of β-casein promoter, cDNA encoding MRJP1 (with bovine β-casein signal peptide) and a Neor selection marker was constructed, and expressed in human MCF-7 cells. To examine the expression of MRJP1, a His-tag protein cDNA fragments were fused on the 5' end of MRJP1 gene using PCR amplification. This vector was shown to be expressed successfully, thus potentially enabling the generation of transgenic cows to be used as mammary gland bioreactors.
{"title":"Construction of a Recombinant Major Royal Jelly Protein 1 (MRJP1) Expression Vector for Mammary Gland-Specific Expression","authors":"Xin Ma, Shubao Yang, W. Jin, Yunjiao Zhao, Hongxia Ma, W. Luan","doi":"10.1109/ICBEB.2012.114","DOIUrl":"https://doi.org/10.1109/ICBEB.2012.114","url":null,"abstract":"Major royal jelly protein 1 (MRJP1, the most abundant protein of honeybee royal jelly) has been recognized as a valuable functional foods and therapeutic protein for human beings. There is great potential using transgenic technology to express recombinant MRJP1 in milk in order to improve the human health conditions. For expression of MRJP1 in milk, mammary gland-specific expression vector (pBC1-MRJP1-NEOr) carrying the expression cassette composed of β-casein promoter, cDNA encoding MRJP1 (with bovine β-casein signal peptide) and a Neor selection marker was constructed, and expressed in human MCF-7 cells. To examine the expression of MRJP1, a His-tag protein cDNA fragments were fused on the 5' end of MRJP1 gene using PCR amplification. This vector was shown to be expressed successfully, thus potentially enabling the generation of transgenic cows to be used as mammary gland bioreactors.","PeriodicalId":6374,"journal":{"name":"2012 International Conference on Biomedical Engineering and Biotechnology","volume":"35 1","pages":"988-991"},"PeriodicalIF":0.0,"publicationDate":"2012-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73175714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study deals with the soil contamination as a reason of the emissions during 50 years work of lead, zinc and sulfuric acid plants of KCM SA. Based on results from 15 monitoring points can be concluded that highest values of the main pollutants are established in the soil from the forest-shelter belt which is very close to the Pb-Zn smelter. There is a tendency for decreasing of soil contamination in the area of KCM SA. It is very good expressed in the zone of maximum distribution of the powder emissions. The realized decreasing of powder emissions on the bases of investments made during past 20 years (more than USD 70 million) in ecological projects gives a very positive effect expressed to a limited soil contamination with heavy nonferrous metals and a tendency to its decreasing.
{"title":"Monitoring of Soil Contamination in Vicinity of Lead-Zinc Smelter KCM SA, Bulgaria","authors":"B. Boyanov, Y. I. Kehayov, K. Ivanov","doi":"10.1109/ICBEB.2012.473","DOIUrl":"https://doi.org/10.1109/ICBEB.2012.473","url":null,"abstract":"This study deals with the soil contamination as a reason of the emissions during 50 years work of lead, zinc and sulfuric acid plants of KCM SA. Based on results from 15 monitoring points can be concluded that highest values of the main pollutants are established in the soil from the forest-shelter belt which is very close to the Pb-Zn smelter. There is a tendency for decreasing of soil contamination in the area of KCM SA. It is very good expressed in the zone of maximum distribution of the powder emissions. The realized decreasing of powder emissions on the bases of investments made during past 20 years (more than USD 70 million) in ecological projects gives a very positive effect expressed to a limited soil contamination with heavy nonferrous metals and a tendency to its decreasing.","PeriodicalId":6374,"journal":{"name":"2012 International Conference on Biomedical Engineering and Biotechnology","volume":"10 1","pages":"385-388"},"PeriodicalIF":0.0,"publicationDate":"2012-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82317850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study explored appropriate method for imaging and detecting early degenerate articular cartilage based on contrast-enhanced Micro-CT. 12 normal articular cartilage samples were prepared from bovine knee. They were immersed in PBS dilutions with specified concentration (20%, 30% and 40% the ionic Meglumine diatrizoate) at 37 °C, and were scanned by the Micro-CT every two hours respectively. Another 6 cartilage samples were digested by trypsin and scanned by contrast-enhanced Micro-CT. For all the AC samples, the X-ray attenuation coefficient of each group reached balance after incubated for 4h in the contrast agent solution. The Micro-CT scanning results showed that the composition and structure of cartilage samples treated with 20% were easier to observe. As the degradation time increased, the degradation degree increased. Under the water bath at 37 °C, the samples can be well imaged via Micro-CT after incubated for more than 4h in 20% contrast agent concentration. It was effective to detect the early degradation by imaging the digested cartilage samples with contrast-enhanced Micro-CT.
{"title":"Exploration of Imaging Method of Degenerate Articular Cartilage Based on EPIC Micro-CT","authors":"Yan Yan, Liang Xiaofei, Cai Xiran, Niu Haijun","doi":"10.1109/ICBEB.2012.206","DOIUrl":"https://doi.org/10.1109/ICBEB.2012.206","url":null,"abstract":"This study explored appropriate method for imaging and detecting early degenerate articular cartilage based on contrast-enhanced Micro-CT. 12 normal articular cartilage samples were prepared from bovine knee. They were immersed in PBS dilutions with specified concentration (20%, 30% and 40% the ionic Meglumine diatrizoate) at 37 °C, and were scanned by the Micro-CT every two hours respectively. Another 6 cartilage samples were digested by trypsin and scanned by contrast-enhanced Micro-CT. For all the AC samples, the X-ray attenuation coefficient of each group reached balance after incubated for 4h in the contrast agent solution. The Micro-CT scanning results showed that the composition and structure of cartilage samples treated with 20% were easier to observe. As the degradation time increased, the degradation degree increased. Under the water bath at 37 °C, the samples can be well imaged via Micro-CT after incubated for more than 4h in 20% contrast agent concentration. It was effective to detect the early degradation by imaging the digested cartilage samples with contrast-enhanced Micro-CT.","PeriodicalId":6374,"journal":{"name":"2012 International Conference on Biomedical Engineering and Biotechnology","volume":"5 1","pages":"797-800"},"PeriodicalIF":0.0,"publicationDate":"2012-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78672467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jin-rui Dong, Ming-hao He, M. Han, Ya-nan Gao, Yu Sun
Rheumatoid arthritis (RA) is a chronic systemic autoimmune disease that principally attacks synovial joints. Patients with RA suffer pain and physical disability. Therefore, scientists are developing many therapies to conquer this disease. Tumor necrosis factor alpha (TNF-a) is one of the key pro-inflammatory cytokine in attending and mediating the inflammatory process of RA. Thus, scientists are trying to develop the drugs targeting at TNF-a for the treatment of RA and have achieved satisfied results in theory and clinical practices. Biological inhibitors are in the lead to be approved to the market. However, the following researches reveal the side effects of this kind of drugs. On the other hand, the small molecular inhibitors are discovered and developed by the scientists, which show great prospects in the treatment of RA. In this review, we will have a brief introduction and outlooks of biological and small molecule TNF-a inhibitors.
{"title":"Development of TNF-alpha Inhibitor Drugs in Treatment of Rheumatoid Arthritis","authors":"Jin-rui Dong, Ming-hao He, M. Han, Ya-nan Gao, Yu Sun","doi":"10.1109/ICBEB.2012.4","DOIUrl":"https://doi.org/10.1109/ICBEB.2012.4","url":null,"abstract":"Rheumatoid arthritis (RA) is a chronic systemic autoimmune disease that principally attacks synovial joints. Patients with RA suffer pain and physical disability. Therefore, scientists are developing many therapies to conquer this disease. Tumor necrosis factor alpha (TNF-a) is one of the key pro-inflammatory cytokine in attending and mediating the inflammatory process of RA. Thus, scientists are trying to develop the drugs targeting at TNF-a for the treatment of RA and have achieved satisfied results in theory and clinical practices. Biological inhibitors are in the lead to be approved to the market. However, the following researches reveal the side effects of this kind of drugs. On the other hand, the small molecular inhibitors are discovered and developed by the scientists, which show great prospects in the treatment of RA. In this review, we will have a brief introduction and outlooks of biological and small molecule TNF-a inhibitors.","PeriodicalId":6374,"journal":{"name":"2012 International Conference on Biomedical Engineering and Biotechnology","volume":"302 1","pages":"149-152"},"PeriodicalIF":0.0,"publicationDate":"2012-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76362622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A strain named n-3 was isolated for its flocculating efficiency could achieve 84.86% from the domestic wastewater of Urtmqi City. The strain was identified as Pseudomonas fragi based on its morphological and 16SrDNA sequence analysis. This study showed that the optimum pH and the optimum temperature for producing bio-flocculants were 7.0 and 30°C respectively.
{"title":"The Screening of Bio-flocculant -producing Bacteria and the Study of its Optimized Culture Conditions and Characteristics of the Bio-flocculant","authors":"Suqin Song, Mei-ying Gu, Jing Zhu, Q. Tang, Zhi-Dong Zhang, Yuqing Xie, Wei Wang","doi":"10.1109/ICBEB.2012.443","DOIUrl":"https://doi.org/10.1109/ICBEB.2012.443","url":null,"abstract":"A strain named n-3 was isolated for its flocculating efficiency could achieve 84.86% from the domestic wastewater of Urtmqi City. The strain was identified as Pseudomonas fragi based on its morphological and 16SrDNA sequence analysis. This study showed that the optimum pH and the optimum temperature for producing bio-flocculants were 7.0 and 30°C respectively.","PeriodicalId":6374,"journal":{"name":"2012 International Conference on Biomedical Engineering and Biotechnology","volume":"61 1","pages":"626-628"},"PeriodicalIF":0.0,"publicationDate":"2012-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86868296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The ultrasonic pressure waveforms produced at the output of medical ultrasound devices must be determined and strictly regulated to ensure patient safety and to ascertain whether the ultrasound devices are performing satisfactorily. However, characterization and measurements of the ultrasound fields are difficult because of the size of the hydrophones' effective aperture. Since the spatial averaging effect mainly depends on the effective diameter of the hydrophone probes, this work explains an experimental technique used to measure the size of the ultrasonic hydrophones' effective aperture. Three ultrasonic transducer sources with three different resonance frequencies, 5 MHz, 7.5 MHz, and 10 MHz were utilized with a pulse-echo technique. The measurements in this work were performed with a bilaminar PVDF membrane hydrophone probe having nominal diameter of 400 μm. The results show that the current measurement system offers the ability to effectively determine the directivity pattern and thus effective diameter for circular aperture membrane hydrophones.
{"title":"Ultrasonic Hydrophone's Effective Aperture Measurements","authors":"S. Umchid, T. Leeudomwong","doi":"10.1109/ICBEB.2012.458","DOIUrl":"https://doi.org/10.1109/ICBEB.2012.458","url":null,"abstract":"The ultrasonic pressure waveforms produced at the output of medical ultrasound devices must be determined and strictly regulated to ensure patient safety and to ascertain whether the ultrasound devices are performing satisfactorily. However, characterization and measurements of the ultrasound fields are difficult because of the size of the hydrophones' effective aperture. Since the spatial averaging effect mainly depends on the effective diameter of the hydrophone probes, this work explains an experimental technique used to measure the size of the ultrasonic hydrophones' effective aperture. Three ultrasonic transducer sources with three different resonance frequencies, 5 MHz, 7.5 MHz, and 10 MHz were utilized with a pulse-echo technique. The measurements in this work were performed with a bilaminar PVDF membrane hydrophone probe having nominal diameter of 400 μm. The results show that the current measurement system offers the ability to effectively determine the directivity pattern and thus effective diameter for circular aperture membrane hydrophones.","PeriodicalId":6374,"journal":{"name":"2012 International Conference on Biomedical Engineering and Biotechnology","volume":"27 1","pages":"1136-1139"},"PeriodicalIF":0.0,"publicationDate":"2012-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88234877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In order to investigate systemic lupus erythematosus (SLE) possible disease biomarkers and disclose its pathogenesis, a rapid resolution liquid chromatography combined with quadrupole-time of flight mass spectrometry method (RRLC-Q-TOF/MS) was used to study the sera from 17 SLE patients and 15 healthy volunteers. Supervised pattern recognition methods combined with orthogonal signal correction partial least square-discriminate analysis (OSC-PLS-DA) were chosen to process the metabolic data. A nice distinction between SLE patients and healthy control group was observed. Further, ten ions, which had a significant contribution to classification, had been selected from metabolic profiles of SLE patients. The ten ions were identified and belong to seven metabolic biomarkers. It was shown that there were abnormal metabolisms of amino acids, phospholipids and porphyrin in SLE patients. It provided a scientific basis for the monitoring and diagnosis of SLE.
{"title":"Serum Metabonomics Study of Systemic Lupus Erythematosus by Rapid Resolution Liquid Chromatography Coupled with Q-TOF Mass Spectrometry","authors":"Xing-hong Ding, Y. Yu","doi":"10.1109/ICBEB.2012.351","DOIUrl":"https://doi.org/10.1109/ICBEB.2012.351","url":null,"abstract":"In order to investigate systemic lupus erythematosus (SLE) possible disease biomarkers and disclose its pathogenesis, a rapid resolution liquid chromatography combined with quadrupole-time of flight mass spectrometry method (RRLC-Q-TOF/MS) was used to study the sera from 17 SLE patients and 15 healthy volunteers. Supervised pattern recognition methods combined with orthogonal signal correction partial least square-discriminate analysis (OSC-PLS-DA) were chosen to process the metabolic data. A nice distinction between SLE patients and healthy control group was observed. Further, ten ions, which had a significant contribution to classification, had been selected from metabolic profiles of SLE patients. The ten ions were identified and belong to seven metabolic biomarkers. It was shown that there were abnormal metabolisms of amino acids, phospholipids and porphyrin in SLE patients. It provided a scientific basis for the monitoring and diagnosis of SLE.","PeriodicalId":6374,"journal":{"name":"2012 International Conference on Biomedical Engineering and Biotechnology","volume":"11 1","pages":"507-510"},"PeriodicalIF":0.0,"publicationDate":"2012-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86487086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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